CN108703260A - A kind of beta carotene bacterium powder and preparation method thereof - Google Patents
A kind of beta carotene bacterium powder and preparation method thereof Download PDFInfo
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Abstract
The present invention provides a kind of preparation methods of beta carotene bacterium powder, this method includes that the thalline containing beta carotene is added in the solution containing emulsifier, filler and antioxidant, shearing obtains beta carotene bacterium solution, is dried to obtain beta carotene bacterium powder.Method provided by the present invention can continue industrialized production, and last handling process and high temperature spray-drying is avoided to cause degradation and isomerization reaction to beta carotene.
Description
Technical field
The present invention relates to feed additive fields, more particularly, to a kind of beta carotene bacterium powder and preparation method thereof.
Background technology
Beta carotene (C40H56) be one of carotenoid and crocus fat-soluble compound, it be in nature most
Generally existing is also most stable of natural pigment.In many natural foods for example：Green vegetables, sweet potato, carrot, spinach, wood
Melon, mango etc. all have abundant beta carotene.Beta carotene is a kind of antioxidant, has detoxication, is to safeguard people
The indispensable nutrient of body health, anticancer, prevention of cardiovascular disease, cataract and it is anti-oxidant on have significant function, and
And then prevent a variety of degenerative disorders caused by aging and aging.As food additives, beta carotene can play pigment and battalion
The effect of hardening agent is supported, while beta carotene is powerful antioxidants, has and inhibit cancer cell multiplication and improve immunity of organisms etc.
Effect.Exclusive nutritive value and medicinal curative effect makes carrotene be paid attention to by countries in the world medical field and grocer.
In the prior art, Chinese invention patent CN105861323A disclose be by beta carotene zymotic fluid through centrifugation or
Sheet frame is separated by solid-liquid separation, and wet thallus, then centrifugal spray drying are obtained, and obtains beta carotene bacterium powder, the invention thalline not into
In the case of row embedding treatment under high temperature spray-drying, beta carotene is caused to be easy to degrade and occur cis-trans isomerism reaction,
Cause economic loss, active reduction.
Invention content
The first object of the present invention is to provide a kind of preparation method of beta carotene bacterium powder, and this method includes that will contain
The thalline of beta carotene is added in the solution containing emulsifier, filler and antioxidant, and shearing obtains beta carotene bacterium
Liquid.
Obtained beta carotene bacterium solution is dried to get the beta carotene bacterium powder.
In a preferred embodiment, the emulsifier be casein sodium, PURE WHEY, converted starch, I
It is one or more in primary glue, gelatin, single diglycerine fatty acid ester, citric acid fatty glyceride, preferably casein sodium,
PURE WHEY, converted starch, Arabic gum or single diglycerine fatty acid ester, further preferably gelatin.
In a preferred embodiment, the filler is corn syrup solid, maltodextrin, glucose syrup, a water
It is one or more in glucose, desalted whey powder, lactose, preferably corn syrup solid, maltodextrin or glucose syrup, into
One step is preferably glucose syrup.Wherein, it is preferable to use the glucose syrups that sugared brix is 70%~80% for glucose syrup.
In a preferred embodiment, the antioxidant be ascorbyl palmitate, it is vitamin E, phosphatide, anti-bad
It is one or more in hematic acid, sodium ascorbate, potassium ascorbate, preferably vitamin E, sodium ascorbate, potassium ascorbate
In one or more, further preferably vitamin E and potassium ascorbate.More preferably, vitamin E and potassium ascorbate
Mass ratio be 1:(1~3).
Wherein, the solution containing emulsifier, filler and antioxidant is to contain emulsifier, filler and antioxidant
Aqueous solution.
In a preferred embodiment, the thalline containing beta carotene uses plate by Blakeslea trispora zymotic fluid
Frame filter pressing obtains.
In one preferred embodiment, the drying means is spray drying, is freeze-dried, vacuum dehydrating at lower temperature,
It is further preferred that the drying means is vacuum dehydrating at lower temperature, dried material temperature is preferably 20~70 DEG C, or spraying is dry
Dry, drying temperature is preferably that inlet air temperature is 180~250 DEG C, and leaving air temp is 80~90 DEG C, or freeze-drying, condenser temperature
It is -40~-20 DEG C.
In a preferred embodiment, in preparation method, in parts by weight, 1~15 part of the emulsifier, filler
2~30 parts, 1~10 part of antioxidant, 20~100 parts of the thalline containing beta carotene.Preferably, the emulsifier 1~10
Part, 2~18 parts of filler, 1~5 part of antioxidant, 20~50 parts of the thalline containing beta carotene.It is further preferred that described
1~8 part of emulsifier, 2~8 parts of filler, 1~3 part of antioxidant, 25~45 parts of the thalline containing beta carotene.
Wherein, the parts by weight can be the unit of weight well known in the art such as μ g, mg, g, kg, can also be its multiple,
Such as 1/10,1/100,10 times, 100 times.In specific embodiment in the present invention, 1 parts by weight are described in detail by taking 1/10kg as an example
The present invention.
Further include to the β-Hu in preparation method to further increase to obtain the shelf life of beta carotene bacterium powder
Sodium alginate is added in radish element bacterium solution.In a preferred embodiment, in parts by weight, sodium alginate can be 1~15
Part.
In a preferred embodiment of the invention, the speed of the shearing is 8000~15000r/min, shearing when
Between be 5~30min.Preferably, the speed of the shearing is 10000~12000r/min, and the time of shearing is 10~20min.
In a preferred embodiment of the invention, the preparation method further include by the beta carotene bacterium solution by from
Heart atomization enters in calcium chloride solution, to form gel microcapsules.Above-mentioned gel microcapsules are dried to get beta carotene again
Bacterium powder.
In a preferred embodiment of the invention, the preparation method of beta carotene bacterium powder of the present invention includes：
The thalline of beta carotene is added in the solution containing gelatin, filler and antioxidant, 8000~
5~30min of 15000r/min down cuts obtains beta carotene bacterium solution, vacuum drying；
The filler is corn syrup solid, maltodextrin or glucose syrup；
The antioxidant is one or more in vitamin E, sodium ascorbate, potassium ascorbate；
In parts by weight, 1~10 part of the emulsifier, 2~18 parts of filler, 1~5 part of antioxidant, contain β-carrot
20~50 parts of the thalline of element.
Wherein, preferably 1~15 part of sodium alginate is added in the solution of gelatin, filler and antioxidant.
Wherein, the thalline containing beta carotene is made by Blakeslea trispora zymotic fluid using plate compression.
Wherein, filler is preferably glucose syrup, and antioxidant is preferably vitamin E and potassium ascorbate, further excellent
It is 1 to be selected as mass ratio:The vitamin E and potassium ascorbate of (1~3).
Wherein, it is highly preferred that 1~10 part of emulsifier, 2~18 parts of filler, 1~5 part of antioxidant, contain β-carrot
20~50 parts of the thalline of element.
Obtained beta carotene bacterium solution can also be entered by centrifugal atomizing in calcium chloride solution, it is micro- to form gel
Capsule.Again by above-mentioned gel microcapsules vacuum dehydrating at lower temperature to get beta carotene bacterium powder.
Wherein, the concentration of calcium chloride solution is preferably 1~10wt%, further preferably 5~10wt%.
It is another object of the present invention to provide the beta carotene bacterium powders that above-mentioned preparation method is prepared.
The content of beta carotene is 1%~10% in the bacterium powder of the beta carotene obtained using the present invention.
Beta carotene thalline is added in the wall material solution containing emulsifier, filler and antioxidant by the present invention, will
Microorganism self-dissolving leads to broken wall and exposed beta carotene grease emulsifying embedding, while continuously being done using preferably vacuum and low temperature
Dry, method provided by the present invention can continue industrialized production, and avoid last handling process and high temperature spray-drying to β-carrot
Element causes degradation and isomerization reaction, the bioactivity and storage stability of the beta carotene bacterium powder that can be improved.
Specific implementation mode
With reference to embodiment, the embodiment of the present invention is furthur described in detail.Following embodiment is used for
Illustrate the present invention, but is not limited to the scope of the present invention.
Embodiment 1
Present embodiments provide a kind of preparation method of beta carotene bacterium powder, including following three steps：
1) beta carotene thalline is prepared
By Blakeslea trispora zymotic fluid (biomass is 40g/L, and content beta-carotene is 1.82g/L), using plate compression
Beta carotene thalline is obtained, it is 1.39% to detect its content beta-carotene, and moisture is 69.07%；
2) beta carotene bacterium solution is prepared
In mixer, 1.89kg pure water is heated to 90 DEG C, puts into 0.5kg converted starches, 1.65kg corn in solids sugar
Then 2.75kg is added with laboratory homomixer in the rate down cut 15min of 10000r/min in slurry and 0.1kg ascorbic acid
Beta carotene thalline obtains beta carotene bacterium solution in the rate down cut 15min of 10000r/min；
3) beta carotene bacterium powder is prepared
Beta carotene bacterium solution is dried by vacuum and low temperature continuous drying equipment, beta carotene bacterium is obtained after crushing
Powder.
After testing, the content beta-carotene of above-mentioned beta carotene bacterium powder is 1.14%, and moisture is 7.7%.
Embodiment 2
Present embodiments provide a kind of preparation method of beta carotene bacterium powder, including following three steps：
1) beta carotene thalline is prepared
By Blakeslea trispora zymotic fluid (biomass is 21.7g/L, and content beta-carotene is 2.85g/L), using sheet frame pressure
Filter obtains beta carotene thalline, and it is 5.65% to detect its content beta-carotene, and moisture is 57%；
2) beta carotene bacterium solution is prepared
In mixer, 2.04kg pure water is heated to 80 DEG C, puts into the mono- diglycerine fatty acid esters of 0.05kg, 0.25kg wheats
Bud dextrin, 0.05kg casein sodiums and 0.2kg sodium ascorbates are cut with laboratory homomixer under the rate of 10000r/min
15min is cut, 4.45kg beta carotene thalline are then added, β-carrot is obtained in the rate down cut 15min of 10000r/min
Plain bacterium solution；
3) beta carotene bacterium powder is prepared
Beta carotene bacterium solution is dried by vacuum and low temperature continuous drying equipment, beta carotene bacterium is obtained after crushing
Powder.
After testing, the content beta-carotene of above-mentioned beta carotene bacterium powder is 10.06%, and moisture is 1.4%.
Embodiment 3
Present embodiments provide a kind of preparation method of beta carotene bacterium powder, including following three steps：
1) beta carotene thalline is prepared
By Blakeslea trispora zymotic fluid (biomass is 23.8g/L, and content beta-carotene is 2.97g/L), using sheet frame pressure
Filter obtains beta carotene thalline, and it is 6.24% to detect its content beta-carotene, and moisture is 50.41%；
2) beta carotene bacterium solution is prepared
In mixer, 1.98kg pure water is heated to 80 DEG C, puts into 0.71kg glucose syrups (sugared brix 70%),
0.1kg PURE WHEYs, 0.1kg sodium ascorbates, 0.1kg vitamin Es and 0.1kg citric acid fatty glycerides, with experiment
Then in the rate down cut 15min of 10000r/min 4.1kg beta carotene thalline are added, in 10000r/min in room mulser
Rate down cut 15min obtain beta carotene bacterium solution；
3) beta carotene bacterium powder is prepared
Beta carotene bacterium solution is dried by vacuum and low temperature continuous drying equipment, beta carotene bacterium is obtained after crushing
Powder.
After testing, the content beta-carotene of above-mentioned beta carotene bacterium powder is 8.25%, and moisture is 5.4%.
Embodiment 4
Present embodiments provide a kind of preparation method of beta carotene bacterium powder, including following three steps：
1) beta carotene thalline is prepared
By Blakeslea trispora zymotic fluid (biomass is 32.5g/L, and content beta-carotene is 2.51g/L), using sheet frame pressure
Filter obtains beta carotene thalline, and it is 2.76% to detect its content beta-carotene, and moisture is 64.78%；
2) beta carotene bacterium solution is prepared
In mixer, 1.73kg pure water is heated to 85 DEG C, puts into 0.25kg converted starches, 0.25kg Arabic gums,
0.5kg corn syrup solids, 0.25kg Dextrose Monohydrates, 0.1kg potassium ascorbates, the mono- diglycerine fatty acid esters of 0.05kg,
0.005kg ascorbyl palmitates, 0.015kg vitamin Es and 0.015kg phosphatide, with laboratory homomixer in 10000r/
Then 3.565kg beta carotene thalline are added, in the rate down cut of 10000r/min in the rate down cut 15min of min
15min obtains beta carotene bacterium solution；
3) beta carotene bacterium powder is prepared
Beta carotene bacterium solution is dried by vacuum and low temperature continuous drying equipment, beta carotene bacterium is obtained after crushing
Powder.
After testing, the content beta-carotene of above-mentioned beta carotene bacterium powder is 3.53%, and moisture is 3.4%.
Embodiment 5
Present embodiments provide a kind of preparation method of beta carotene bacterium powder, including following three steps：
1) beta carotene thalline is prepared
By Blakeslea trispora zymotic fluid (biomass is 25.4g/L, and content beta-carotene is 2.88g/L), using sheet frame pressure
Filter obtains beta carotene thalline, and it is 4.48% to detect its content beta-carotene, and moisture is 60.21%；
2) beta carotene bacterium solution is prepared
In mixer, 1.70kg pure water is heated to 80 DEG C, puts into 0.5kg maltodextrins, 0.25kg lactose, 0.2kg
Sodium ascorbate, 0.1kg casein sodiums and 0.1kg PURE WHEYs, with laboratory homomixer 10000r/min rate
Then down cut 15min is added 3.85kg beta carotene thalline, β-Hu is obtained in the rate down cut 15min of 10000r/min
Radish element bacterium solution；
3) beta carotene bacterium powder is prepared
Beta carotene bacterium solution is dried by vacuum and low temperature continuous drying equipment, beta carotene bacterium is obtained after crushing
Powder.
After testing, the content beta-carotene of above-mentioned beta carotene bacterium powder is 6.20%, and moisture is 3.6%.
Embodiment 6
Present embodiments provide a kind of preparation method of beta carotene bacterium powder, including following three steps：
1) beta carotene thalline is prepared
By Blakeslea trispora zymotic fluid (biomass is 30.3g/L, and content beta-carotene is 2.83g/L), using sheet frame pressure
Filter obtains beta carotene thalline, and it is 3.92% to detect its content beta-carotene, and moisture is 58.26%；
2) beta carotene bacterium solution is prepared
In mixer, 1.97kg pure water is heated to 70 DEG C, 0.5kg gelatin is first put into and is swollen 0.5h, then put into 0.5kg
Desalted whey powder and 0.2kg potassium ascorbates, with laboratory homomixer 10000r/min rate down cut 15min, then
3.8kg beta carotene thalline are added, beta carotene bacterium solution is obtained in the rate down cut 15min of 10000r/min；
3) beta carotene bacterium powder is prepared
Beta carotene bacterium solution is dried by spray drying device, beta carotene bacterium powder is obtained after crushing.
After testing, the content beta-carotene of above-mentioned beta carotene bacterium powder is 5.15%, and moisture is 3.6%.
Embodiment 7
Present embodiments provide a kind of preparation method of beta carotene bacterium powder, including following three steps：
1) beta carotene thalline is prepared
By Blakeslea trispora zymotic fluid (biomass is 34.9g/L, and content beta-carotene is 2.34g/L), using sheet frame pressure
Filter obtains beta carotene thalline, and it is 3.09% to detect its content beta-carotene, and moisture is 53.69%；
2) beta carotene bacterium solution is prepared
In mixer, 4.78kg pure water is heated to 70 DEG C, 0.75kg gelatin is first put into and is swollen 0.5h, then put into
0.75kg sodium alginates, 0.71kg glucose syrups (sugared brix 70%), 0.1kg vitamin Es and 0.1kg potassium ascorbates, with reality
Room mulser is tested in the rate down cut 15min of 10000r/min, 2.8kg beta carotene thalline are then added, in 10000r/
The rate down cut 15min of min obtains beta carotene bacterium solution；
3) beta carotene bacterium powder is prepared
Beta carotene bacterium solution is entered into 5%CaCl by centrifugal atomizing2In solution, gel microcapsules are formed, gel is micro-
Capsule is washed with deionized, filters, and is dried by vacuum and low temperature continuous drying equipment, obtains beta carotene bacterium powder.
After testing, the content beta-carotene of above-mentioned beta carotene bacterium powder is 2.31%, and moisture is 6.8%.
Embodiment 8
Present embodiments provide a kind of preparation method of beta carotene bacterium powder, including following three steps：
1) beta carotene thalline is prepared
By Blakeslea trispora zymotic fluid (biomass is 34.9g/L, and content beta-carotene is 2.34g/L), using sheet frame pressure
Filter obtains beta carotene thalline, and it is 3.09% to detect its content beta-carotene, and moisture is 53.69%；
2) beta carotene bacterium solution is prepared
In mixer, 5.76kg pure water is heated to 70 DEG C, 0.25kg gelatin is first put into and is swollen 0.5h, then put into
0.25kg sodium alginates, 1.43kg glucose syrups (sugared brix 70%), 0.1kg vitamin Es and 0.1kg potassium ascorbates, with reality
Room mulser is tested in the rate down cut 15min of 8000r/min, 3.3kg beta carotene thalline are then added, in 8000r/min
Rate down cut 15min obtain beta carotene bacterium solution；
3) beta carotene bacterium powder is prepared
Beta carotene bacterium solution is entered into 5%CaCl by centrifugal atomizing2In solution, gel microcapsules are formed, gel is micro-
Capsule is washed with deionized, filters, and is dried by vacuum and low temperature continuous drying equipment, obtains beta carotene bacterium powder.
After testing, the content beta-carotene of above-mentioned beta carotene bacterium powder is 3.0%, and moisture is 5.0%.
Comparative example 1
This comparative example provides a kind of preparation method of beta carotene bacterium powder, including following three steps：
1) beta carotene thalline is prepared
By Blakeslea trispora zymotic fluid (biomass is 40g/L, and content beta-carotene is 1.82g/L), using plate compression
Beta carotene thalline is obtained, it is 1.39% to detect its content beta-carotene, and moisture is 69.07%；
2) beta carotene bacterium solution is prepared
In mixer, 6.01kg pure water is heated to 70 DEG C, is added 0.75kg sodium alginates, 0.1kg vitamin Es and
Then 4.05kg β-are added with laboratory homomixer in the rate down cut 15min of 10000r/min in 0.1kg potassium ascorbates
Carrotene thalline obtains beta carotene bacterium solution in the rate down cut 15min of 10000r/min；
3) beta carotene bacterium powder is prepared
Beta carotene bacterium solution is entered into 5%CaCl by centrifugal atomizing2In solution, gel microcapsules are formed, gel is micro-
Capsule is washed with deionized, filters, and is dried by vacuum and low temperature continuous drying equipment, obtains beta carotene bacterium powder.
After testing, the content beta-carotene of above-mentioned beta carotene bacterium powder is 2.4%, and moisture is 6.2%.
Comparative example 2
This comparative example provides a kind of preparation method of beta carotene bacterium powder, including following three steps：
1) beta carotene thalline is prepared
By Blakeslea trispora zymotic fluid (biomass is 34.7g/L, and content beta-carotene is 1.91g/L), using sheet frame pressure
Filter obtains beta carotene thalline, and it is 1.99% to detect its content beta-carotene, and moisture is 63.33%；
2) beta carotene bacterium solution is prepared
In mixer, 6.01kg pure water is heated to 70 DEG C, puts into 0.75kg sodium alginates, 0.71kg glucose syrups
(sugared brix 70%), 0.1kg vitamin Es and 0.1kg potassium ascorbates, with laboratory homomixer under the rate of 10000r/min
15min is sheared, 3.55kg beta carotene thalline are then added, β-Hu Luo are obtained in the rate down cut 15min of 10000r/min
Bu Su bacterium solutions；
3) beta carotene bacterium powder is prepared
Beta carotene bacterium solution is entered into 5%CaCl by centrifugal atomizing2In solution, gel microcapsules are formed, gel is micro-
Capsule is washed with deionized, filters, and is dried by vacuum and low temperature continuous drying equipment, obtains beta carotene bacterium powder.
After testing, the content beta-carotene of above-mentioned beta carotene bacterium powder is 2.43%, and moisture is 5.24%.
Comparative example 3
This comparative example provides a kind of preparation method of beta carotene bacterium powder, including following three steps：
1) beta carotene thalline is prepared
By Blakeslea trispora zymotic fluid (biomass is 56.9g/L, and content beta-carotene is 1.25g/L), using sheet frame pressure
Filter obtains beta carotene thalline, and it is 1.06% to detect its content beta-carotene, and moisture is 50.27%；
2) beta carotene bacterium solution is prepared
In mixer, 1.97kg pure water is heated to 90 DEG C, puts into 0.5kg converted starches and 0.1kg ascorbic acid, is used
Then 4.4kg beta carotene thalline are added in the rate down cut 15min of 10000r/min in laboratory homomixer,
The rate down cut 15min of 10000r/min obtains beta carotene bacterium solution；
3) beta carotene bacterium powder is prepared
Beta carotene bacterium solution is dried by vacuum and low temperature continuous drying equipment, beta carotene bacterium is obtained after crushing
Powder.
After testing, the content beta-carotene of above-mentioned beta carotene bacterium powder is 1.53%, and moisture is 8.9%.
The beta carotene bacterium powder of Examples 1 to 8 and comparative example 1~3 uses aluminium foil bag vacuum pack with nitrogen, at 37 DEG C
Under the conditions of carry out shelf life experiment, experimental data is as follows：
1 shelf life experimental data of embodiment
2 shelf life experimental data of embodiment
| Initially | 1 month | 2 months | 3 months | 4 months | 5 months | 6 months | |
| Content | 10.06% | 9.89% | 9.84% | 9.66% | 9.48% | 9.43% | 9.25% |
| The dough softening | 1.69% | 2.19% | 3.98% | 5.77% | 6.26% | 8.05% |
3 shelf life experimental data of embodiment
| Initially | 1 month | 2 months | 3 months | 4 months | 5 months | 6 months | |
| Content | 8.25% | 8.10% | 7.99% | 7.87% | 7.78% | 7.62% | 7.53% |
| The dough softening | 1.82% | 3.15% | 4.61% | 5.70% | 7.64% | 8.73% |
4 shelf life experimental data of embodiment
| Initially | 1 month | 2 months | 3 months | 4 months | 5 months | 6 months | |
| Content | 3.53% | 3.44% | 3.37% | 3.35% | 3.30% | 3.25% | 3.21% |
| The dough softening | 2.55% | 4.53% | 5.10% | 6.52% | 7.93% | 9.07% |
5 shelf life experimental data of embodiment
| Initially | 1 month | 2 months | 3 months | 4 months | 5 months | 6 months | |
| Content | 6.20% | 6.10% | 6.03% | 5.98% | 5.88% | 5.84% | 5.74% |
| The dough softening | 1.61% | 2.74% | 3.55% | 5.16% | 5.81% | 7.42% |
6 shelf life experimental data of embodiment
| Initially | 1 month | 2 months | 3 months | 4 months | 5 months | 6 months | |
| Content | 5.15% | 5.00% | 4.93% | 4.87% | 4.75% | 4.70% | 4.64% |
| The dough softening | 2.91% | 4.27% | 5.44% | 7.77% | 8.74% | 9.90% |
7 shelf life experimental data of embodiment
| Initially | 1 month | 2 months | 3 months | 4 months | 5 months | 6 months | |
| Content | 2.31% | 2.30% | 2.27% | 2.26% | 2.29% | 2.28% | 2.26% |
| The dough softening | 0.43% | 1.73% | 2.16% | 1.08% | 1.30% | 2.16% |
8 shelf life experimental data of embodiment
| Initially | 1 month | 2 months | 3 months | 4 months | 5 months | 6 months | |
| Content | 3.00% | 2.97% | 2.93% | 2.95% | 2.94% | 2.91% | 2.90% |
| The dough softening | 1.00% | 2.33% | 1.65% | 2.14% | 3.00% | 3.33% |
1 shelf life experimental data of comparative example
| Initially | 1 month | 2 months | 3 months | 4 months | 5 months | 6 months | |
| Content | 2.40% | 2.31% | 2.28% | 2.23% | 2.20% | 2.15% | 2.06% |
| The dough softening | 3.75% | 5.00% | 7.08% | 8.33% | 10.42% | 14.17% |
2 shelf life experimental data of comparative example
| Initially | 1 month | 2 months | 3 months | 4 months | 5 months | 6 months | |
| Content | 2.43% | 2.35% | 2.31% | 2.29% | 2.25% | 2.20% | 2.11% |
| The dough softening | 3.29% | 4.94% | 5.76% | 7.41% | 9.47% | 13.17% |
3 shelf life experimental data of comparative example
| Initially | 1 month | 2 months | 3 months | 4 months | 5 months | 6 months | |
| Content | 1.52% | 1.47% | 1.45% | 1.42% | 1.39% | 1.37% | 1.31% |
| The dough softening | 3.29% | 4.61% | 6.58% | 8.55% | 9.87% | 13.82% |
Finally, method of the invention is only preferable embodiment, is not intended to limit the scope of the present invention.It is all
Within the spirit and principles in the present invention, any modification, equivalent replacement, improvement and so on should be included in the protection of the present invention
Within the scope of.
Claims (10)
1. a kind of preparation method of beta carotene bacterium powder, which is characterized in that including the thalline containing beta carotene to be added to
In solution containing emulsifier, filler and antioxidant, shearing obtains beta carotene bacterium solution, is dried to obtain the β-carrot
Plain bacterium powder.
2. preparation method according to claim 1, which is characterized in that the emulsifier is casein sodium, lactalbumin
It is one or more in powder, converted starch, Arabic gum, gelatin, single diglycerine fatty acid ester, citric acid fatty glyceride,
Preferably casein sodium, PURE WHEY, converted starch, Arabic gum or single diglycerine fatty acid ester, further preferably
Gelatin；
And/or the filler is corn syrup solid, maltodextrin, glucose syrup, Dextrose Monohydrate, desalted whey powder, breast
It is one or more in sugar, preferably corn syrup solid, maltodextrin or glucose syrup, further preferably glucose syrup；
And/or the antioxidant is ascorbyl palmitate, vitamin E, phosphatide, ascorbic acid, sodium ascorbate, resists
It is one or more in bad hematic acid potassium, it is preferably one or more in vitamin E, sodium ascorbate, potassium ascorbate, into one
Step is preferably vitamin E and potassium ascorbate.
3. preparation method according to claim 1 or 2, which is characterized in that the thalline containing beta carotene is by three spores
The mould zymotic fluid of Bradley is made using plate compression；And/or the drying means is preferably spray-dried, is freeze-dried, vacuum is low
Temperature is dry.
4. preparation method according to any one of claim 1 to 3, which is characterized in that in parts by weight, the emulsifier
1~15 part, 2~30 parts of filler, 1~10 part of antioxidant, 20~100 parts of the thalline containing beta carotene；Preferably, institute
State 1~10 part of emulsifier, 2~18 parts of filler, 1~5 part of antioxidant, 20~50 parts of the thalline containing beta carotene.
5. preparation method according to any one of claim 1 to 4, which is characterized in that further include to the beta carotene
Sodium alginate is added in bacterium solution.
6. preparation method according to claim 5, which is characterized in that in parts by weight, the sodium alginate is 1~15
Part.
7. preparation method according to any one of claim 1 to 6, which is characterized in that the speed of the shearing is 8000
The time of~15000r/min, shearing are 5~30min；Preferably, the speed of the shearing is 10000~12000r/min, is cut
The time cut is 10~20min.
8. preparation method according to any one of claim 1 to 7, which is characterized in that the preparation method includes：
The thalline of beta carotene is added in the solution containing gelatin, filler and antioxidant, in 8000~15000r/
5~30min of min down cuts obtains beta carotene bacterium solution, vacuum drying；
The filler is corn syrup solid, maltodextrin or glucose syrup；
The antioxidant is one or more in vitamin E, sodium ascorbate, potassium ascorbate；
In parts by weight, 1~10 part of the emulsifier, 2~18 parts of filler, 1~5 part of antioxidant, contain beta carotene
20~50 parts of thalline.
9. preparation method according to any one of claim 1 to 8, which is characterized in that further include by the beta carotene
Bacterium solution is entered by centrifugal atomizing in calcium chloride solution, to form gel microcapsules.
10. the beta carotene bacterium powder that the preparation method described in any one of claim 1 to 9 is prepared.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110810655A (en) * | 2019-12-03 | 2020-02-21 | 嘉必优生物技术（武汉）股份有限公司 | Blakeslea trispora feed additive and application thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103005156A (en) * | 2012-12-05 | 2013-04-03 | 浙江大学 | Preparation method of beta-carotene for microencapsulation feed |
| CN103406079A (en) * | 2013-06-28 | 2013-11-27 | 大连医诺生物有限公司 | Method for preparing cold water dispersing type carotenoid micro-capsule powder without using organic solvent |
| CN105747216A (en) * | 2016-03-03 | 2016-07-13 | 广州市食品工业研究所有限公司 | Microcapsules-beta and carotene powder and preparation method thereof |
| CN105861323A (en) * | 2014-11-17 | 2016-08-17 | 嘉必优生物技术（武汉）股份有限公司 | Beta-carotene-containing bacterial powder, microbial oil and oil suspension |
| CN106666714A (en) * | 2016-12-29 | 2017-05-17 | 厦门金达威生物科技有限公司 | Sugar-free beta-carotene microcapsules and preparation method thereof |
| CN107048367A (en) * | 2017-04-24 | 2017-08-18 | 广州市食品工业研究所有限公司 | A kind of natural Beta-carotene microscapsule powder and preparation method thereof |
| CN108148444A (en) * | 2017-12-29 | 2018-06-12 | 嘉必优生物技术（武汉）股份有限公司 | A kind of high-content beta carotene microcapsules for being used for coloring and preparation method thereof |
-
2018
- 2018-07-16 CN CN201810778946.9A patent/CN108703260A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103005156A (en) * | 2012-12-05 | 2013-04-03 | 浙江大学 | Preparation method of beta-carotene for microencapsulation feed |
| CN103406079A (en) * | 2013-06-28 | 2013-11-27 | 大连医诺生物有限公司 | Method for preparing cold water dispersing type carotenoid micro-capsule powder without using organic solvent |
| CN105861323A (en) * | 2014-11-17 | 2016-08-17 | 嘉必优生物技术（武汉）股份有限公司 | Beta-carotene-containing bacterial powder, microbial oil and oil suspension |
| CN105747216A (en) * | 2016-03-03 | 2016-07-13 | 广州市食品工业研究所有限公司 | Microcapsules-beta and carotene powder and preparation method thereof |
| CN106666714A (en) * | 2016-12-29 | 2017-05-17 | 厦门金达威生物科技有限公司 | Sugar-free beta-carotene microcapsules and preparation method thereof |
| CN107048367A (en) * | 2017-04-24 | 2017-08-18 | 广州市食品工业研究所有限公司 | A kind of natural Beta-carotene microscapsule powder and preparation method thereof |
| CN108148444A (en) * | 2017-12-29 | 2018-06-12 | 嘉必优生物技术（武汉）股份有限公司 | A kind of high-content beta carotene microcapsules for being used for coloring and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110810655A (en) * | 2019-12-03 | 2020-02-21 | 嘉必优生物技术（武汉）股份有限公司 | Blakeslea trispora feed additive and application thereof |
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