CN105861323A - Beta-carotene-containing bacterial powder, microbial oil and oil suspension - Google Patents
Beta-carotene-containing bacterial powder, microbial oil and oil suspension Download PDFInfo
- Publication number
- CN105861323A CN105861323A CN201610276071.3A CN201610276071A CN105861323A CN 105861323 A CN105861323 A CN 105861323A CN 201610276071 A CN201610276071 A CN 201610276071A CN 105861323 A CN105861323 A CN 105861323A
- Authority
- CN
- China
- Prior art keywords
- blakeslea trispora
- beta carotene
- cctcc
- carotene
- oil
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000013734 beta-carotene Nutrition 0.000 title claims abstract description 81
- 239000011648 beta-carotene Substances 0.000 title claims abstract description 81
- OENHQHLEOONYIE-JLTXGRSLSA-N β-Carotene Chemical compound CC=1CCCC(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C OENHQHLEOONYIE-JLTXGRSLSA-N 0.000 title claims abstract description 81
- OENHQHLEOONYIE-UKMVMLAPSA-N all-trans beta-carotene Natural products CC=1CCCC(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C OENHQHLEOONYIE-UKMVMLAPSA-N 0.000 title claims abstract description 80
- TUPZEYHYWIEDIH-WAIFQNFQSA-N beta-carotene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C)C=CC=C(/C)C=CC2=CCCCC2(C)C TUPZEYHYWIEDIH-WAIFQNFQSA-N 0.000 title claims abstract description 80
- 229960002747 betacarotene Drugs 0.000 title claims abstract description 80
- 239000000843 powder Substances 0.000 title claims abstract description 16
- 230000000813 microbial effect Effects 0.000 title claims abstract description 14
- 239000012053 oil suspension Substances 0.000 title claims abstract description 10
- 230000001580 bacterial effect Effects 0.000 title abstract description 42
- 241000235553 Blakeslea trispora Species 0.000 claims abstract description 50
- OAIJSZIZWZSQBC-GYZMGTAESA-N lycopene Chemical compound CC(C)=CCC\C(C)=C\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C=C(/C)CCC=C(C)C OAIJSZIZWZSQBC-GYZMGTAESA-N 0.000 claims abstract description 50
- 238000000855 fermentation Methods 0.000 claims abstract description 39
- 230000004151 fermentation Effects 0.000 claims abstract description 34
- 238000004321 preservation Methods 0.000 claims abstract description 8
- 241000894006 Bacteria Species 0.000 claims description 26
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 238000005516 engineering process Methods 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 238000012258 culturing Methods 0.000 abstract 1
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 72
- 239000001963 growth medium Substances 0.000 description 22
- 238000011218 seed culture Methods 0.000 description 21
- 238000000034 method Methods 0.000 description 17
- 230000031700 light absorption Effects 0.000 description 15
- 238000010899 nucleation Methods 0.000 description 15
- 239000007788 liquid Substances 0.000 description 14
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 12
- 239000003921 oil Substances 0.000 description 12
- 235000019198 oils Nutrition 0.000 description 12
- 239000000243 solution Substances 0.000 description 11
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 10
- 239000002609 medium Substances 0.000 description 10
- 238000001514 detection method Methods 0.000 description 9
- 239000012530 fluid Substances 0.000 description 9
- 239000001965 potato dextrose agar Substances 0.000 description 9
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 8
- 238000011088 calibration curve Methods 0.000 description 8
- 239000004744 fabric Substances 0.000 description 8
- 238000002360 preparation method Methods 0.000 description 8
- 239000000725 suspension Substances 0.000 description 8
- 235000015112 vegetable and seed oil Nutrition 0.000 description 8
- 239000008158 vegetable oil Substances 0.000 description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 7
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 6
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 5
- 240000008042 Zea mays Species 0.000 description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 5
- 229910052799 carbon Inorganic materials 0.000 description 5
- 235000005822 corn Nutrition 0.000 description 5
- 239000000284 extract Substances 0.000 description 5
- 239000008103 glucose Substances 0.000 description 5
- 239000002054 inoculum Substances 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 229910052757 nitrogen Inorganic materials 0.000 description 5
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 4
- 238000002835 absorbance Methods 0.000 description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 4
- 239000013078 crystal Substances 0.000 description 4
- 235000013312 flour Nutrition 0.000 description 4
- 238000011068 loading method Methods 0.000 description 4
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 4
- 235000019341 magnesium sulphate Nutrition 0.000 description 4
- 238000003801 milling Methods 0.000 description 4
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 4
- 235000019796 monopotassium phosphate Nutrition 0.000 description 4
- 239000012452 mother liquor Substances 0.000 description 4
- 229910052760 oxygen Inorganic materials 0.000 description 4
- 239000001301 oxygen Substances 0.000 description 4
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 4
- 239000002504 physiological saline solution Substances 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- 238000010008 shearing Methods 0.000 description 4
- 238000003786 synthesis reaction Methods 0.000 description 4
- 238000005303 weighing Methods 0.000 description 4
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000004807 desolvation Methods 0.000 description 2
- 235000019441 ethanol Nutrition 0.000 description 2
- 238000010884 ion-beam technique Methods 0.000 description 2
- 238000002386 leaching Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000003094 microcapsule Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000000049 pigment Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000235548 Blakeslea Species 0.000 description 1
- 229920002261 Corn starch Polymers 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 244000000626 Daucus carota Species 0.000 description 1
- 235000002767 Daucus carota Nutrition 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- QAQJMLQRFWZOBN-LAUBAEHRSA-N L-ascorbyl-6-palmitate Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](O)[C@H]1OC(=O)C(O)=C1O QAQJMLQRFWZOBN-LAUBAEHRSA-N 0.000 description 1
- 239000011786 L-ascorbyl-6-palmitate Substances 0.000 description 1
- 229920002774 Maltodextrin Polymers 0.000 description 1
- 239000005913 Maltodextrin Substances 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 240000007711 Peperomia pellucida Species 0.000 description 1
- 235000012364 Peperomia pellucida Nutrition 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 235000019486 Sunflower oil Nutrition 0.000 description 1
- 241000282458 Ursus sp. Species 0.000 description 1
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 235000010385 ascorbyl palmitate Nutrition 0.000 description 1
- 238000000889 atomisation Methods 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000002285 corn oil Substances 0.000 description 1
- 235000005687 corn oil Nutrition 0.000 description 1
- 239000008120 corn starch Substances 0.000 description 1
- 229940099112 cornstarch Drugs 0.000 description 1
- 235000012343 cottonseed oil Nutrition 0.000 description 1
- 239000002385 cottonseed oil Substances 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 229940035034 maltodextrin Drugs 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 239000000155 melt Substances 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000002600 sunflower oil Substances 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 238000001291 vacuum drying Methods 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
- 239000011719 vitamin A Substances 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Botany (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention provides beta-carotene-containing bacterial powder or microbial oil or oil suspension. Beta-carotene is obtained by fermenting a blakeslea trispora mutant strain. The beta-carotene-containing bacterial powder or microbial oil or oil suspension is characterized in that the blakeslea trispora mutant strain is preserved in the China Center For Type Culture Collection (CCTCC) on August 8, 2014, the preservation address is Wuhan University, Wuhan city, China, and the preservation numbers are blakeslea trispora BT7251(+) CCTCC M 2014378 and blakeslea trispora BT7603(-) CCTCC M 2014379. The beta-carotene with the high yield can be obtained by conventionally fermenting and culturing the blakeslea trispora mutant strain. Meanwhile, the whole fermentation technology is simple, convenient to control and beneficial for industrialized production.
Description
Technical field
The present invention relates to bacterium powder, microbial oil and the oil suspension containing beta carotene.
Background technology
Beta carotene is the one in more than 500 kind of carrotene, is the precursor of vitamin A, also referred to as provitamin A, it
Weak solution is orange-yellow to yellow.Beta carotene is widely present in plant, algae and fungi, but can not in animal and human body
Synthesis carrotene, it is necessary to absorb from the external world.As food additives, beta carotene can play the work of pigment and nutrition fortifier
With, beta carotene is powerful antioxidants simultaneously, has inhibition cancer cell propagation and improves the effects such as immunity of organisms.Exclusive
Nutritive value and medicinal curative effect make carrotene be paid attention to by countries in the world medical circle and grocer.
The preparation method of beta carotene mainly includes following several at present: one, chemical synthesis, i.e. uses organic chemical industry former
Material, by chemosynthesis reaction, Prof. Du Yucang beta carotene.Chemical synthesis production carrotene technical sophistication, difficulty are big, alive
Property low, strong toxicity, it is impossible to be absorbed by the body completely, and to human body produce a certain degree of toxic and side effect, many countries limit
Use.Two, plant extraction method, i.e. from plant material, such as uses organic solvent in the plant such as carrot, as petroleum ether, chloroform,
The extraction beta carotene such as acetone, ether, ethanol.But, use the method to need to consume substantial amounts of raw material, and extract yield
The highest, it is difficult to be adapted to large-scale industrial production.Three, biological fermentation process, i.e. utilizes microbial culture technique, by micro-life
The cultivation of thing, utilizes microorganism at its internal synthesis beta carotene, then isolated beta carotene in microbial body.Should
The production cost of method is low, and beta carotene product purity is high, and the rate of recovery is high, up to more than 90%.Therefore, microorganism is used to send out
Ferment method produces beta carotene and has broad application prospects.Particularly, with Blakeslea trispora bacterial strain fermenting and producing β mono-carrotene,
The security of its technique is high, with low cost, and colorability is strong, therefore suffers from the especially favor of production firm.
Number of patent application be 201210208891.0 Chinese patent application disclose one and utilize trispore Bruce mould
The method of beta carotene is prepared in fermentation, including by trispore Bruce mould ATCC14271 (+) and ATCC14272 (-) activation after,
Through seed culture, send out than accessing in fermentation medium by certain inoculum concentration and inoculation under preferred technological condition for fermentation
Ferment, finally gives the mycelium that content beta-carotene is higher.But, the beta carotene yield using this bacterial classification to prepare is relatively low,
Maximum output is less than 1.3g/L.
Summary of the invention
The main object of the present invention is to provide a kind of bacterium powder, microbial oil and oil suspension containing beta carotene.
For reaching above-mentioned purpose, the present invention provides the bacterium powder containing beta carotene, and described beta carotene is by three spore Bradleys
The fermentation of mould mutant strain is obtained, it is characterised in that: described Blakeslea trispora mutant strain is preserved in China's allusion quotation on August 8th, 2014
Type culture collection center (CCTCC), preservation address is, China, Wuhan, Wuhan University, deposit number is: three spore cloth Laplaces are mould
Blakeslea trispora BT7251(+)CCTCCM 2014378；Three spore cloth Laplace mould Blakeslea trispora
BT7603(-)CCTCC M 2014379；Or
Containing the microbial oil of beta carotene, described beta carotene is obtained by the fermentation of Blakeslea trispora mutant strain, its
It is characterised by: described Blakeslea trispora mutant strain is preserved in China typical culture collection center on August 8th, 2014
(CCTCC), preservation address is, China, Wuhan, Wuhan University, deposit number is: the three spore mould Blakeslea of cloth Laplace
trispora BT7251(+)CCTCC M 2014378；Three spore cloth Laplace mould Blakeslea trispora BT7603 (-)
CCTCC M 2014379.Or；
Containing the oil suspension of beta carotene, described beta carotene is obtained by the fermentation of Blakeslea trispora mutant strain, and it is special
Levy and be: described Blakeslea trispora mutant strain is preserved in China typical culture collection center (CCTCC) on August 8th, 2014,
Preservation address is, China, Wuhan, Wuhan University, deposit number is: three spore cloth Laplace mould Blakeslea trispora
BT7251(+)CCTCC M 2014378；Three spore cloth Laplace mould Blakeslea trispora BT7603 (-) CCTCC M
2014379。
The beneficial effects of the present invention is: after Blakeslea trispora mutant strain carries out the fermented and cultured of routine, can obtain higher
The beta carotene of yield.Meanwhile, whole zymotechnique is simple, it is simple to control, beneficially industrialized production.
Detailed description of the invention
In order to be more clearly understood that the technology contents of the present invention, describe in detail especially exemplified by following example.
The selection of Blakeslea trispora mutant strain
1) take the positive bacterium of commercially available Blakeslea trispora (deposit number is: CCTCCAF97006 (+)) and Blakeslea trispora bear bacterium
(deposit number is: CCTCCAF96002 (-)) it is starting strain.
2) two strain starting strains are cultivated 5 days to spore maturation.
3) obtain pure spore liquid by gauze or Filter paper filtering, spore liquid is injected into sterile petri dish, through sterile wind
Air-dry, become bacterial plaque.By in aseptic for the culture dish containing bacterial plaque immigration high energy particle beam implanter, inject through high energy ion beam and lure
Become.
4) bacterial plaque of positive and negative bacterial strain is eluted with sterilized water respectively, it is thus achieved that positive bacterium and negative bacterium respectively according to man-to-man
Relation is inoculated in distance center 1/3 distance on potato dextrose agar (PDA) culture medium flat plate, in 27 DEG C, humidity
The constant incubator of 50% is cultivated 5 days, and now visible positive and negative bacterial strain stolon combines in the center of flat board, produces
Raw red material.
5) with the card punch of 30mm, potato dextrose agar (PDA) culture medium of plate center is taken out, be placed in glass
Smashing to pieces in test tube, the ethyl acetate adding 5ml soaks, until the redness of agar takes off to the greatest extent, takes upper strata ethyl acetate detection pigment and exists
Light absorption value at 455nm.
6) from the higher sample of light absorption value, select 30 flat boards, carry out second and take turns high energy ion beam injection mutagenesis, then carry out
The operation of the 4th and the 5th step, obtains 3 flat boards that light absorption value is higher.
7) obtaining the three positive and negative bacterial strains of strain from 3 flat boards, the 3 positive and negative bacterial strains of strain carry out liquid submerged culture, according to shaking flask
The result of fermented and cultured, the positive and negative bacterial strain that screening content beta-carotene is the highest, it is thus achieved that the Blakeslea trispora mutant strain of the present invention.
This Blakeslea trispora bacterial strain is preserved in China typical culture collection center (CCTCC) on August 8th, 2014, and preservation address is,
China, Wuhan, Wuhan University, deposit number: three spore cloth Laplace mould Blakeslea trispora BT7251 (+) CCTCC M
2014378；Three spore cloth Laplace mould Blakeslea trispora BT7603 (-) CCTCC M 2014379.
8) it has following physio-biochemical characteristics:
1, positive and negative bacteria growing is in PDA slant medium, after growing 2 days, can form white in media surface at 27 DEG C
Bacterium colony, and enter exponential phase, positive strain mycelia is light yellow, and negative strain mycelia foresythia, within the 4th day, mycelia is covered with whole inclined-plane.
2, sporogenesis was in the 3rd day, within the 4th day, was covered with whole inclined-plane；Positive strain spore is black, the most loose, and negative bacterium spore is less,
The most intensive.
3, in the culture medium cultivated, available carbon source is: glucose, sucrose, cornstarch etc..Available nitrogen
Source is mainly dusty yeast, yeast extract, yeast leaching powder, soybean cake powder or corn flour.
The application of Blakeslea trispora bacterial strain of the present invention
Hereinafter all utilize the Blakeslea trispora bacterial strain of the present invention as initial bacterial classification, use different preparation methods to prepare
Beta carotene.
Method 1
(1) actication of culture: under gnotobasis, positive and negative for Blakeslea trispora bacterial strain is inoculated in inclined-plane PDA culture medium respectively,
It is placed in incubator, cultivates 5d, after the positive and negative bacterial strain of Blakeslea trispora grows spore respectively, aseptically, by nothing for 27 DEG C
Slant medium miospore is scraped by bacterium physiological saline, and is configured to uniform spore suspension, makes positive and negative bacterial strain spore hang
The concentration of liquid respectively reaches: 103～106Individual spore/mL, 103～106Individual spore/mL.
(2) seed culture: positive and negative bacterial strain is inoculated in seed culture medium respectively in the way of spore suspension, seed culture
Base is contained in the triangular flask of 1000mL, liquid amount 100mL, and cultivation temperature is 27 DEG C, rotating speed 220r/min, when positive and negative bacterium is cultivated
Between be 18 hours, obtain Blakeslea trispora positive and negative bacterial strain seed liquor.
(3) seed expands cultivation: the volume of final fermentation tank is 2m3, selection volume is the seeding tank of 10L, 100L successively
Expand cultivate seed liquor, in seeding tank, culture medium loading amount is 60% (volume ratio), by the shake-flask seed zymotic fluid of step (2) according to
Positive and negative bacterial strain ratio is that the ratio of 1:10 is inoculated in seeding tank and carries out seed and expand and cultivate, and wherein seed culture medium is: carbon source
For glucose；Nitrogen source is dusty yeast and corn flour；Inorganic salts are potassium dihydrogen phosphate, magnesium sulfate；Vegetable oil is: corn oil.
(4) fermented and cultured: in seeding tank, bacterium is dense reach 20% after, be linked into equipped with 1.2m by culture transferring pipeline3Fermentation training
Support the 2m of base3Fermentation tank is cultivated, fermentation medium with seed culture medium prescription, inoculum concentration 5% (volume ratio), temperature:
27℃±1℃；Rotating speed: 200r/min；Air quantity: 2vvm；Dissolved oxygen controls: more than 20%；Cycle: 120h；Tank pressure: 0.12Mpa.pH
Being adjusted to 6.8 by NaOH, before 72, stream adds the vegetable oil of 40g/L.
(5) detection.
A, standard curve making
Take 10mg beta carotene standard items, be dissolved in 1L ethyl acetate, make mother liquor.Take respectively 2.5ml, 5ml,
7.5ml, 10ml, 12.5ml are settled to 25ml.Measure beta carotene each concentration light absorption value 455nm at, according to light absorption value with
The concentration of beta carotene is calibration curve y=ax+b (x unit is mg/L), R2=0.999.
B, fermentation broth sample process and detection
A. v will be taken1ML zymotic fluid filtered through gauze also press dry with tablet press machine, obtains wet thallus, weighs wet thallus weight in wet base, note
Record weight ω 1.Calculate thalline weight in wet base concentration c1(g/L)=1000*w1/v1。
B. weigh wet thallus 0.02-0.03 gram, weigh and record weight w2(g)。
C. the wet thallus after weighing is placed in homogenizer, in homogenizer add 3ml ethyl acetate milling and extracting until
Till dreg is colourless.
D. extract is moved in 25 milliliters of volumetric flasks, be settled to scale with ethyl acetate and shake up.
E. draw 1 milliliter of above-mentioned solution with 1 milliliter of fat tripe suction pipe, be transferred in 10 milliliters of volumetric flasks, fixed with ethyl acetate
Appearance to scale shakes up.
F. at 454nm, sample absorbance y is detected with visible spectrophotometer1。
G. the content of beta carotene in ethyl acetate solution is calculated according to light absorption value and calibration curve.
x1(mg/L)=(y1-b)/a
H. beta carotene fermentation yield computing formula is: P (g/L)=x1*10*0.025*c1/w2/1000。
If i. without step e, then yield formula is P (g/L)=x1*0.025*c1/w2/1000。
Finally recording beta carotene fermentation yield is 3.2g/L.
Method 2
(1) actication of culture: under gnotobasis, positive and negative for Blakeslea trispora bacterial strain is inoculated in inclined-plane PDA culture medium respectively,
It is placed in incubator, cultivates 5d, after the positive and negative bacterial strain of Blakeslea trispora grows spore respectively, aseptically, by nothing for 27 DEG C
Slant medium miospore is scraped by bacterium physiological saline, and is configured to uniform spore suspension, makes positive and negative bacterial strain spore hang
The concentration of liquid respectively reaches: 103～106Individual spore/mL, 103～106Individual spore/mL.
(2) seed culture: positive and negative bacterial strain is inoculated in seed culture medium respectively in the way of spore suspension, seed culture
Base is contained in the triangular flask of 1000mL, liquid amount 100mL, and cultivation temperature is 27 DEG C, rotating speed 220r/min, when positive and negative bacterium is cultivated
Between be 18 hours, obtain Blakeslea trispora positive and negative bacterial strain seed liquor.
(3) seed expands cultivation: the volume of final fermentation tank is 12m3, selecting volume successively is 10L, 100L, 2m3Kind
Sub-tank expands cultivates seed liquor, and in seeding tank, culture medium loading amount is 60% (volume ratio), by the shake-flask seed zymotic fluid of step (2)
Being inoculated in seeding tank according to the ratio that positive and negative bacterial strain ratio is 1:15 and carry out seed expansion cultivation, wherein seed culture medium is:
Carbon source is glucose；Nitrogen source is yeast leaching powder and corn flour；Inorganic salts are potassium dihydrogen phosphate, magnesium sulfate；Vegetable oil is: cottonseed
Oil.
(4) fermented and cultured: in seeding tank, bacterium is dense reach 20% after, be linked into equipped with 7m by culture transferring pipeline3Fermented and cultured
The 12m of base3Fermentation tank is cultivated, fermentation medium with seed culture medium prescription, inoculum concentration 15% (volume ratio), temperature:
27℃±1℃；Rotating speed: 200r/min；Air quantity: 2vvm；Dissolved oxygen controls: more than 25%；Cycle: 120h；Tank pressure: 0.12Mpa.Logical
Crossing NaOH and be adjusted to 6.8, before 72, stream adds the vegetable oil of 40g/L.
(5) detection.
A, standard curve making
Take 10mg beta carotene standard items, be dissolved in 1L ethyl acetate, make mother liquor.Take respectively 2.5ml, 5ml,
7.5ml, 10ml, 12.5ml are settled to 25ml.Measure beta carotene each concentration light absorption value 455nm at, according to light absorption value with
The concentration of beta carotene is calibration curve y=ax+b (x unit is mg/L), R2=0.999.
B, fermentation broth sample process and detection
A. v will be taken1ML zymotic fluid filtered through gauze also press dry with tablet press machine, obtains wet thallus, weighs wet thallus weight in wet base, note
Record weight ω 1.Calculate thalline weight in wet base concentration c1(g/L)=1000*w1/v1。
B. weigh wet thallus 0.02-0.03 gram, weigh and record weight w2(g)。
C. the wet thallus after weighing is placed in homogenizer, in homogenizer add 3ml ethyl acetate milling and extracting until
Till dreg is colourless.
D. extract is moved in 25 milliliters of volumetric flasks, be settled to scale with ethyl acetate and shake up.
E. draw 1 milliliter of above-mentioned solution with 1 milliliter of fat tripe suction pipe, be transferred in 10 milliliters of volumetric flasks, fixed with ethyl acetate
Appearance to scale shakes up.
F. at 454nm, sample absorbance y is detected with visible spectrophotometer1。
G. the content of beta carotene in ethyl acetate solution is calculated according to light absorption value and calibration curve.
x1(mg/L)=(y1-b)/a
H. beta carotene fermentation yield computing formula is: P (g/L)=x1*10*0.025*c1/w2/1000。
If i. without step e, then yield formula is P (g/L)=x1*0.025*c1/w2/1000.Finally record β-Hu Luo
Bu Su fermentation yield is 3.5g/L.
Method 3
(1) actication of culture；Under gnotobasis, positive and negative for Blakeslea trispora bacterial strain is inoculated in inclined-plane PDA culture medium respectively,
It is placed in incubator, cultivates 5d, after the positive and negative bacterial strain of Blakeslea trispora grows spore respectively, aseptically, by nothing for 27 DEG C
Slant medium miospore is scraped by bacterium physiological saline, and is configured to uniform spore suspension, makes positive and negative bacterial strain spore hang
The concentration of liquid respectively reaches: 103～106Individual spore/mL, 103～106Individual spore/mL.
(2) seed culture；Positive and negative bacterial strain is inoculated in seed culture medium respectively in the way of spore suspension, seed culture
Base is contained in the triangular flask of 1000mL, liquid amount 100mL, and cultivation temperature is 27 DEG C, rotating speed 220r/min, when positive and negative bacterium is cultivated
Between be 18 hours, obtain Blakeslea trispora positive and negative bacterial strain seed liquor.
(3) seed expands cultivation；The volume of final fermentation tank is 45m3, selecting volume successively is 10L, 100L, 2m3, 12m3
Seeding tank expand cultivate seed liquor, in seeding tank, culture medium loading amount is 60% (volume ratio), is sent out by the shake-flask seed of step (2)
Ferment liquid is inoculated in seeding tank according to the ratio that positive and negative bacterial strain ratio is 1:20 and carries out seed expansion cultivation, wherein seed culture
Base is: carbon source is glucose；Nitrogen source is dusty yeast and corn flour；Inorganic salts are potassium dihydrogen phosphate, magnesium sulfate；Vegetable oil is: hair
Soya-bean oil.
(4) fermented and cultured；In seeding tank, bacterium is dense reach 20% after, be linked into equipped with 25m by culture transferring pipeline3Fermented and cultured
The 50m of base3Fermentation tank is cultivated, fermentation medium with seed culture medium prescription, inoculum concentration 20% (volume ratio), temperature:
27℃±1℃；Rotating speed: 200r/min；Air quantity: 2vvm；Dissolved oxygen controls: more than 30%；Cycle: 120h；Tank pressure: 0.12Mpa.Logical
Crossing NaOH and be adjusted to 6.8, before 72, stream adds the vegetable oil of 40g/L.
(5) detection:
A, standard curve making
Take 10mg beta carotene standard items, be dissolved in 1L ethyl acetate, make mother liquor.Take respectively 2.5ml, 5ml,
7.5ml, 10ml, 12.5ml are settled to 25ml.Measure beta carotene each concentration light absorption value 455nm at, according to light absorption value with
The concentration of beta carotene is calibration curve y=ax+b (x unit is mg/L), R2=0.999.
B, fermentation broth sample process and detection
A. v will be taken1ML zymotic fluid filtered through gauze also press dry with tablet press machine, obtains wet thallus, weighs wet thallus weight in wet base, note
Record weight ω 1.Calculate thalline weight in wet base concentration c1(g/L)=1000*w1/v1。
B. weigh wet thallus 0.02-0.03 gram, weigh and record weight w2(g)。
C. the wet thallus after weighing is placed in homogenizer, in homogenizer add 3ml ethyl acetate milling and extracting until
Till dreg is colourless.
D. extract is moved in 25 milliliters of volumetric flasks, be settled to scale with ethyl acetate and shake up.
E. draw 1 milliliter of above-mentioned solution with 1 milliliter of fat tripe suction pipe, be transferred in 10 milliliters of volumetric flasks, fixed with ethyl acetate
Appearance to scale shakes up.
F. at 454nm, sample absorbance y is detected with visible spectrophotometer1。
G. the content of beta carotene in ethyl acetate solution is calculated according to light absorption value and calibration curve.
x1(mg/L)=(y1-b)/a。
H. beta carotene fermentation yield computing formula is: P (g/L)=x1*10*0.025*c1/w2/1000。
If i. without step e, then yield formula is P (g/L)=x1*0.025*c1/w2/1000.Finally record β-Hu Luo
Bu Su fermentation yield: 4.6g/L.
Method 4
(1) actication of culture: under gnotobasis, positive and negative for Blakeslea trispora bacterial strain is inoculated in inclined-plane PDA culture medium respectively,
It is placed in incubator, cultivates 5d, after the positive and negative bacterial strain of Blakeslea trispora grows spore respectively, aseptically, by nothing for 27 DEG C
Slant medium miospore is scraped by bacterium physiological saline, and is configured to uniform spore suspension, makes positive and negative bacterial strain spore hang
The concentration of liquid respectively reaches: 103～106Individual spore/mL, 103～106Individual spore/mL.
(2) seed culture: positive and negative bacterial strain is inoculated in seed culture medium respectively in the way of spore suspension, seed culture
Base is contained in the triangular flask of 1000mL, liquid amount 100mL, and cultivation temperature is 27 DEG C, rotating speed 220r/min, when positive and negative bacterium is cultivated
Between be 18 hours, obtain Blakeslea trispora positive and negative bacterial strain seed liquor.
(3) seed expands cultivation: the volume of final fermentation tank is 200m3, selecting volume successively is 10L, 100L, 5m3,
50m3Seeding tank expand cultivate seed liquor, in seeding tank, culture medium loading amount is 60% (volume ratio), by the shaking flask kind of step (2)
Sub-zymotic fluid is inoculated in seeding tank according to the ratio that positive and negative bacterial strain ratio is 1:25 and carries out seed expansion cultivation, wherein seed
Culture medium is: carbon source is glucose；Nitrogen source is dusty yeast and beancake powder；Inorganic salts are potassium dihydrogen phosphate, magnesium sulfate；Vegetable oil
For: hair cotton oil.
(4) fermented and cultured: in seeding tank, bacterium is dense reach 20% after, be linked into equipped with 120m by culture transferring pipeline3Fermentation training
Support the 200m of base3Cultivating in fermentation tank, fermentation medium is with seed culture medium prescription, inoculum concentration 25% (volume ratio), temperature
Degree: 27 DEG C ± 1 DEG C；Rotating speed: 200r/min；Air quantity: 2vvm；Dissolved oxygen controls: more than 35%；Cycle: 120h；Tank pressure:
0.12Mpa.Being adjusted to 6.8 by NaOH, before 72, stream adds the vegetable oil of 40g/L.
(5) detection:
A, standard curve making
Take 10mg beta carotene standard items, be dissolved in 1L ethyl acetate, make mother liquor.Take respectively 2.5ml, 5ml,
7.5ml, 10ml, 12.5ml are settled to 25ml.Measure beta carotene each concentration light absorption value 455nm at, according to light absorption value with
The concentration of beta carotene is calibration curve y=ax+b (x unit is mg/L), R2=0.999.
B, fermentation broth sample process and detection
A. v will be taken1ML zymotic fluid filtered through gauze also press dry with tablet press machine, obtains wet thallus, weighs wet thallus weight in wet base, note
Record weight ω 1.Calculate thalline weight in wet base concentration c1(g/L)=1000*w1/v1。
B. weigh wet thallus 0.02-0.03 gram, weigh and record weight w2(g)。
C. the wet thallus after weighing is placed in homogenizer, in homogenizer add 3ml ethyl acetate milling and extracting until
Till dreg is colourless.
D. extract is moved in 25 milliliters of volumetric flasks, be settled to scale with ethyl acetate and shake up.
E. draw 1 milliliter of above-mentioned solution with 1 milliliter of fat tripe suction pipe, be transferred in 10 milliliters of volumetric flasks, fixed with ethyl acetate
Appearance to scale shakes up.
F. at 454nm, sample absorbance y is detected with visible spectrophotometer1。
G. the content of beta carotene in ethyl acetate solution is calculated according to light absorption value and calibration curve.
x1(mg/L)=(y1-b)/a
H. beta carotene fermentation yield computing formula is: P (g/L)=x1*10*0.025*c1/w2/1000。
If i. without step e, then yield formula is P (g/L)=x1*0.025*c1/w2/1000.Finally record β-Hu Luo
Bu Su fermentation yield: 5.2g/L.
As can be seen from the above embodiments, Blakeslea trispora of the present invention positive and negative bacterial strain mutant strain is used to prepare β-carrot
Element, its technique is simple, beta carotene yield is high, and is convenient for industrialized production.
The preparation bacterium powder containing beta carotene.
By the beta carotene fermentation liquor obtained by any of the above method is centrifugal or sheet frame carries out separation of solid and liquid, obtain wet
Thalline.Use the mode of centrifugal spray to be dried according to following parameter and i.e. obtain beta carotene bacterium powder.
| Rotary speed rpm | Charging rate kg/h | EAT DEG C | Leaving air temp DEG C | Air quantity m3/h |
| 4000 | 70 | 190 | 80 | 3300 |
The preparation microbial oil containing beta carotene.
By the beta carotene fermentation liquor obtained by any of the above method is centrifugal or sheet frame carries out separation of solid and liquid, obtain wet
Thalline, adds the absolute ethyl alcohol of 1:10 (m/v), with broken 1 hour of colloid mill circulation after mixing, is filtrated to get ethanol filtrate and breaks
Broken wet thallus.Broken wet thallus adds the ethyl acetate of 1:10 (m/v), extraction three times under 55 degrees Celsius, each 1 hour.Every time
It is filtrated to get solvent phase and filter residue.Collect solvent phase, after vacuum desolvation, obtain the microbial oil containing beta carotene.
The preparation crystal containing beta carotene.
By the aforementioned prepared microbial oil containing beta carotene adds the ethyl acetate of 1:1 (m/v), at 5 degrees Celsius
Under be slowly stirred crystallization 4 hours, filter, obtain containing beta carotene coarse crystal.Further by vacuum drying desolvation,
Obtain containing beta carotene crystal finished product.
The preparation oil suspension containing beta carotene.
Aforementioned prepared beta carotene crystal finished product according to target weight ratio (such as 30%) is mixed with sunflower oil, takes out
Vacuum, is heated to 150 degrees Celsius, stirs 10 minutes, and after beta carotene melts and mixes, fast cooling is to room temperature.I.e.
Prepare beta carotene oil suspension.
The preparation microcapsules containing beta carotene.
First, wall material solution is obtained according to following formula through the cutter shearing of 3500rpm.
| Raw material | Weight kg |
| Converted starch | 30 |
| Corn syrup solid | 25 |
| Maltodextrin | 25 |
| Ascorbyl palmitate | 2 |
| Vitamin E | 2 |
| Pure water | 90 |
Then, limit sheared edge add the aforementioned prepared microbial oil 16kg containing beta carotene, after having added
Continue under the rotating speed of 8000rpm to shear 10min.After shearing terminates, carrying out homogeneous under 40MPa, the homogenizing fluid obtained is following
Under the conditions of carry out press spray and i.e. can get the microcapsules of beta carotene.
| Atomisation pressure MPa | Charging rate kg/h | EAT DEG C | Leaving air temp DEG C | Air quantity m3/h |
| 10 | 100 | 220 | 90 | 5000 |
The preparation fatty powder containing beta carotene.
First, the aforementioned prepared microbial oil 16kg containing beta carotene is used at normal temperatures with DHA algal oil 10kg
Stirring 100rpm mixing 10min, obtains compound lard.
Then, wall material solution is obtained according to following formula through the cutter shearing of 4500rpm.
Then, limit sheared edge add the aforementioned prepared miscella 26kg containing beta carotene and DHA, after having added
Continue under the rotating speed of 10000rpm to shear 15min.After shearing terminates, carrying out homogeneous under 40MPa, the homogenizing fluid obtained is following
Under the conditions of be centrifuged spraying and i.e. can get the mixing-in fat powder of beta carotene and DHA.
| Rotary speed rpm | Charging rate kg/h | EAT DEG C | Leaving air temp DEG C | Air quantity m3/h |
| 5000 | 90 | 200 | 85 | 4500 |
Claims (3)
1. containing the bacterium powder of beta carotene, described beta carotene is obtained by the fermentation of Blakeslea trispora mutant strain, and its feature exists
In: described Blakeslea trispora mutant strain is preserved in China typical culture collection center (CCTCC), preservation on August 8th, 2014
Address is, China, Wuhan, Wuhan University, and deposit number is: Blakeslea trispora BT7251 (+) CCTCC M 2014378;Three
The mould BT7603 of spore Bradley (-) CCTCC M 2014379.
2. containing the microbial oil of beta carotene, described beta carotene is obtained by the fermentation of Blakeslea trispora mutant strain, and it is special
Levy and be: described Blakeslea trispora mutant strain is preserved in China typical culture collection center (CCTCC) on August 8th, 2014,
Preservation address is, China, Wuhan, Wuhan University, and deposit number is: Blakeslea trispora BT7251 (+) CCTCC M
2014378;Blakeslea trispora BT7603 (-) CCTCC M 2014379.
3. containing the oil suspension of beta carotene, described beta carotene is obtained by the fermentation of Blakeslea trispora mutant strain, its feature
It is: described Blakeslea trispora mutant strain is preserved in China typical culture collection center (CCTCC) on August 8th, 2014, protects
Address, Tibetan is, China, Wuhan, Wuhan University, and deposit number is: Blakeslea trispora BT7251 (+) CCTCC M 2014378;
Blakeslea trispora BT7603 (-) CCTCC M 2014379.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610276071.3A CN105861323A (en) | 2014-11-17 | 2014-11-17 | Beta-carotene-containing bacterial powder, microbial oil and oil suspension |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410649170.2A CN104531538A (en) | 2014-11-17 | 2014-11-17 | Blakeslea trispora mutant strain and application thereof |
| CN201610276071.3A CN105861323A (en) | 2014-11-17 | 2014-11-17 | Beta-carotene-containing bacterial powder, microbial oil and oil suspension |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410649170.2A Division CN104531538A (en) | 2014-11-17 | 2014-11-17 | Blakeslea trispora mutant strain and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105861323A true CN105861323A (en) | 2016-08-17 |
Family
ID=52847173
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610276071.3A Pending CN105861323A (en) | 2014-11-17 | 2014-11-17 | Beta-carotene-containing bacterial powder, microbial oil and oil suspension |
| CN201410649170.2A Pending CN104531538A (en) | 2014-11-17 | 2014-11-17 | Blakeslea trispora mutant strain and application thereof |
| CN201610276069.6A Active CN105925653B (en) | 2014-11-17 | 2014-11-17 | Microcapsules and fatty powder containing beta carotene |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410649170.2A Pending CN104531538A (en) | 2014-11-17 | 2014-11-17 | Blakeslea trispora mutant strain and application thereof |
| CN201610276069.6A Active CN105925653B (en) | 2014-11-17 | 2014-11-17 | Microcapsules and fatty powder containing beta carotene |
Country Status (1)
| Country | Link |
|---|---|
| CN (3) | CN105861323A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107827798A (en) * | 2017-11-21 | 2018-03-23 | 嘉必优生物技术（武汉）股份有限公司 | A kind of bata-carotene and preparation method thereof, application |
| CN107827799A (en) * | 2017-11-21 | 2018-03-23 | 嘉必优生物技术（武汉）股份有限公司 | A kind of bata-carotene and preparation method thereof, application |
| CN108703260A (en) * | 2018-07-16 | 2018-10-26 | 嘉必优生物技术（武汉）股份有限公司 | A kind of beta carotene bacterium powder and preparation method thereof |
| CN109456903A (en) * | 2018-12-29 | 2019-03-12 | 嘉必优生物技术（武汉）股份有限公司 | Selenium-rich richness carotenoid trispore Bruce mould, fermentative carotenoid product and fermentation process |
| CN112553281A (en) * | 2020-12-28 | 2021-03-26 | 嘉必优生物技术（武汉）股份有限公司 | Fermentation medium and application thereof |
| CN116144510A (en) * | 2023-04-06 | 2023-05-23 | 中国海洋大学 | Aureobasidium pullulans and application thereof in improving beta-carotene content by fermentation |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105506048B (en) * | 2015-12-31 | 2019-06-21 | 内蒙古金达威药业有限公司 | A kind of fermentation process preparing beta carotene using Blakeslea trispora |
| CN106278977A (en) * | 2016-08-09 | 2017-01-04 | 嘉必优生物技术（武汉）股份有限公司 | The method preparing bata-carotene crystal |
| CN106278976A (en) * | 2016-08-09 | 2017-01-04 | 嘉必优生物技术（武汉）股份有限公司 | The method preparing bata-carotene crystal |
| CN106278975A (en) * | 2016-08-09 | 2017-01-04 | 嘉必优生物技术（武汉）股份有限公司 | The method preparing bata-carotene crystal |
| CN106278978A (en) * | 2016-08-09 | 2017-01-04 | 嘉必优生物技术（武汉）股份有限公司 | The method preparing bata-carotene crystal |
| CN107604036B (en) * | 2017-09-22 | 2020-10-02 | 嘉必优生物技术（武汉）股份有限公司 | Method for preparing beta-carotene and beta-carotene product |
| CN109536558B (en) * | 2017-09-22 | 2021-11-19 | 嘉必优生物技术（武汉）股份有限公司 | Process for preparing beta-carotene |
| CN108559768B (en) * | 2018-01-11 | 2021-07-09 | 嘉必优生物技术（武汉）股份有限公司 | Screening method and application of microorganisms containing MVA and/or MEP metabolic pathways |
| CN111690541B (en) * | 2019-03-13 | 2023-04-25 | 浙江创新生物有限公司 | Paired natural beta-carotene high-yield strain, and preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1193048A (en) * | 1998-03-23 | 1998-09-16 | 江苏省微生物研究所 | Method for producing beta-carotene by fermentation |
| CN102787158A (en) * | 2011-05-20 | 2012-11-21 | 浙江医药股份有限公司新昌制药厂 | Method for producing natural beta-carotene by fermentation and application |
| CN103911288A (en) * | 2013-01-08 | 2014-07-09 | 丰益(上海)生物技术研发中心有限公司 | Method for preconcentration and wall breaking and extraction of microorganism oil |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR0129228B1 (en) * | 1994-04-01 | 1998-04-09 | 구자홍 | Automatic cooking control method & device of microwave |
| DE10300649A1 (en) * | 2003-01-09 | 2004-07-22 | Basf Ag | Process for the production of ketocarotenoids by cultivating genetically modified organisms |
| CN100423652C (en) * | 2003-12-03 | 2008-10-08 | 广州优宝工业有限公司 | Natural beta-carotene microcapsule and method for making same |
-
2014
- 2014-11-17 CN CN201610276071.3A patent/CN105861323A/en active Pending
- 2014-11-17 CN CN201410649170.2A patent/CN104531538A/en active Pending
- 2014-11-17 CN CN201610276069.6A patent/CN105925653B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1193048A (en) * | 1998-03-23 | 1998-09-16 | 江苏省微生物研究所 | Method for producing beta-carotene by fermentation |
| CN102787158A (en) * | 2011-05-20 | 2012-11-21 | 浙江医药股份有限公司新昌制药厂 | Method for producing natural beta-carotene by fermentation and application |
| CN103911288A (en) * | 2013-01-08 | 2014-07-09 | 丰益(上海)生物技术研发中心有限公司 | Method for preconcentration and wall breaking and extraction of microorganism oil |
Non-Patent Citations (2)
| Title |
|---|
| 李建东: "β-胡萝卜素微胶囊化工艺研究", 《万方》 * |
| 谭新国 等: "三孢布拉霉发酵生产β-胡萝卜素", 《中南民族学院学报（自然科学版）》 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107827798A (en) * | 2017-11-21 | 2018-03-23 | 嘉必优生物技术（武汉）股份有限公司 | A kind of bata-carotene and preparation method thereof, application |
| CN107827799A (en) * | 2017-11-21 | 2018-03-23 | 嘉必优生物技术（武汉）股份有限公司 | A kind of bata-carotene and preparation method thereof, application |
| CN107827798B (en) * | 2017-11-21 | 2020-05-08 | 嘉必优生物技术（武汉）股份有限公司 | β -carotene and preparation method and application thereof |
| CN107827799B (en) * | 2017-11-21 | 2020-09-01 | 嘉必优生物技术（武汉）股份有限公司 | Beta-carotene and preparation method and application thereof |
| CN108703260A (en) * | 2018-07-16 | 2018-10-26 | 嘉必优生物技术（武汉）股份有限公司 | A kind of beta carotene bacterium powder and preparation method thereof |
| CN109456903A (en) * | 2018-12-29 | 2019-03-12 | 嘉必优生物技术（武汉）股份有限公司 | Selenium-rich richness carotenoid trispore Bruce mould, fermentative carotenoid product and fermentation process |
| CN109456903B (en) * | 2018-12-29 | 2021-04-13 | 嘉必优生物技术（武汉）股份有限公司 | Selenium-rich carotenoid-rich Blakeslea trispora, carotenoid fermentation product and fermentation method |
| CN112553281A (en) * | 2020-12-28 | 2021-03-26 | 嘉必优生物技术（武汉）股份有限公司 | Fermentation medium and application thereof |
| CN116144510A (en) * | 2023-04-06 | 2023-05-23 | 中国海洋大学 | Aureobasidium pullulans and application thereof in improving beta-carotene content by fermentation |
| CN116144510B (en) * | 2023-04-06 | 2024-06-07 | 中国海洋大学 | Aureobasidium pullulans and application thereof in improving beta-carotene content by fermentation |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104531538A (en) | 2015-04-22 |
| CN105925653B (en) | 2019-10-15 |
| CN105925653A (en) | 2016-09-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105925653B (en) | Microcapsules and fatty powder containing beta carotene | |
| CN102787158B (en) | Method for producing natural beta-carotene by fermentation and application | |
| CN104012298B (en) | The complete production of hybrid seeds technique of edible fungus liquid kind submerged fermentation and culture medium prescription thereof | |
| CN101870739B (en) | Paenibacillus polymyxa extracellular polysaccharide and application thereof | |
| CN102220270B (en) | Screening method for producing chondroitin sulfate bacterial strain and application of bacterial strain fermentation method in production of chondroitin sulfate | |
| CN102154407B (en) | Corayceps militaris polysaccharide two-stage fermentation synthesis process | |
| CN104893983B (en) | Liquid state fermentation low citrinin, the preparation method of High color values monascorubin and product | |
| CN107699500A (en) | The method of aureobasidium pullulans and its fermenting and producing pulullan polysaccharide | |
| CN107156638B (en) | Preparation method of lipid-lowering red yeast powder | |
| CN103468463A (en) | Production method of monascus liquid state fermentation yeast | |
| CN103898016A (en) | High-yield lactic acid bacterium and method for preparing calcium lactate by fermenting eggshells with same | |
| CN103695315B (en) | A kind of fermentable produces the method for chitin oligosaccharide | |
| Yang et al. | The ultrasonic effect on biological characteristics of Monascus sp. | |
| CN106434466B (en) | A kind of Rhodococcus ruber and the preparation method and application thereof generating natural red colouring matter | |
| CN107604036A (en) | A kind of method for preparing bata-carotene and bata-carotene product | |
| CN107384879B (en) | A kind of fermentation manufacturing technique of recombinant yeast pichia pastoris bacterium production liver detoxification enzyme | |
| CN106190898A (en) | A kind of industrialization liquid fermentation method of the bacillus cereus JZBC1 dissolving pond dinoflagellate | |
| CN109644778A (en) | A kind of edible fungus liquid fermentation medium and preparation method thereof | |
| CN109321617A (en) | A method of Natamycin is synthesized using industrial or agricultural by-product solid state fermentation | |
| CN104726515A (en) | Method for extracting bacterial exopolysaccharide rich in fucose | |
| CN108796027A (en) | A method of producing carotenoid | |
| CN104046674A (en) | Modified corn steep liquor for fermentation production of beta-carotene, and preparation method and application thereof | |
| CN104543402A (en) | Preparation method of fermentation-state iron feed additive | |
| CN101838667B (en) | Method for producing lycopene by fermentation of blakeslea trispora | |
| CN109957590A (en) | A kind of natamycin synthesis technology based on mycelium pellet structure regulating |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160817 |
|
| RJ01 | Rejection of invention patent application after publication |