CN108699597A - 单分子对照 - Google Patents

单分子对照 Download PDF

Info

Publication number
CN108699597A
CN108699597A CN201680080249.5A CN201680080249A CN108699597A CN 108699597 A CN108699597 A CN 108699597A CN 201680080249 A CN201680080249 A CN 201680080249A CN 108699597 A CN108699597 A CN 108699597A
Authority
CN
China
Prior art keywords
nucleic acid
area
solid support
amplification
binding site
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201680080249.5A
Other languages
English (en)
Chinese (zh)
Inventor
布莱恩·麦考恩
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DNAE Group Holdings Ltd
Original Assignee
DNAE Group Holdings Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB1520883.8A external-priority patent/GB201520883D0/en
Priority claimed from GBGB1605055.1A external-priority patent/GB201605055D0/en
Application filed by DNAE Group Holdings Ltd filed Critical DNAE Group Holdings Ltd
Publication of CN108699597A publication Critical patent/CN108699597A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201680080249.5A 2015-11-26 2016-11-23 单分子对照 Pending CN108699597A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GB1520883.8 2015-11-26
GBGB1520883.8A GB201520883D0 (en) 2015-11-26 2015-11-26 Single molecule controls
GB1605055.1 2016-03-24
GBGB1605055.1A GB201605055D0 (en) 2016-03-24 2016-03-24 Single molecule controls
PCT/GB2016/053649 WO2017089767A1 (en) 2015-11-26 2016-11-23 Single molecule controls

Publications (1)

Publication Number Publication Date
CN108699597A true CN108699597A (zh) 2018-10-23

Family

ID=57442739

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201680080249.5A Pending CN108699597A (zh) 2015-11-26 2016-11-23 单分子对照

Country Status (5)

Country Link
US (1) US20210285035A1 (enExample)
EP (1) EP3380634A1 (enExample)
JP (1) JP2018534942A (enExample)
CN (1) CN108699597A (enExample)
WO (1) WO2017089767A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2015217208B2 (en) 2014-02-11 2018-08-30 The Regents Of The University Of Colorado, A Body Corporate CRISPR enabled multiplexed genome engineering
DK3474669T3 (da) 2016-06-24 2022-06-27 Univ Colorado Regents Fremgangsmåde til generering af stregkodede kombinatoriske biblioteker
US10011849B1 (en) 2017-06-23 2018-07-03 Inscripta, Inc. Nucleic acid-guided nucleases
US9982279B1 (en) 2017-06-23 2018-05-29 Inscripta, Inc. Nucleic acid-guided nucleases

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999014376A2 (en) * 1997-09-19 1999-03-25 Genaco Biomedical Products, Inc. Detection of aneuploidy and gene deletion by pcr-based gene-dose co-amplification of chromosome specific sequences with synthetic internal controls
US20020172965A1 (en) * 1996-12-13 2002-11-21 Arcaris, Inc. Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom
US20040072150A1 (en) * 2002-06-12 2004-04-15 Venkatakrishna Shyamala Identification of oligonucleotides for the capture, detection and quantitation of hepatitis A viral nucleic acid
CN101067156A (zh) * 2007-05-18 2007-11-07 中国人民解放军第三军医大学第一附属医院 一种基于选择性探针的多重pcr方法及其应用
US20080108515A1 (en) * 2004-02-10 2008-05-08 Gormley Niall A Arrayed polynucleotides
CN101821619A (zh) * 2007-09-07 2010-09-01 弗卢丁公司 拷贝数变化确定、方法和系统

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060040300A1 (en) * 2004-08-09 2006-02-23 Generation Biotech, Llc Method for nucleic acid isolation and amplification
US9347092B2 (en) * 2009-02-25 2016-05-24 Roche Molecular System, Inc. Solid support for high-throughput nucleic acid analysis
US9416409B2 (en) * 2009-07-31 2016-08-16 Ibis Biosciences, Inc. Capture primers and capture sequence linked solid supports for molecular diagnostic tests
JP5635130B2 (ja) * 2011-01-26 2014-12-03 株式会社日立ハイテクノロジーズ 単分子プローブ核酸付き微粒子及びその製造方法、並びに核酸分析方法
DE102011085473A1 (de) * 2011-10-28 2013-05-02 Albert-Ludwigs-Universität Freiburg Verfahren zur Identifikation von Aptameren

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020172965A1 (en) * 1996-12-13 2002-11-21 Arcaris, Inc. Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom
WO1999014376A2 (en) * 1997-09-19 1999-03-25 Genaco Biomedical Products, Inc. Detection of aneuploidy and gene deletion by pcr-based gene-dose co-amplification of chromosome specific sequences with synthetic internal controls
US20040072150A1 (en) * 2002-06-12 2004-04-15 Venkatakrishna Shyamala Identification of oligonucleotides for the capture, detection and quantitation of hepatitis A viral nucleic acid
US20080108515A1 (en) * 2004-02-10 2008-05-08 Gormley Niall A Arrayed polynucleotides
CN101067156A (zh) * 2007-05-18 2007-11-07 中国人民解放军第三军医大学第一附属医院 一种基于选择性探针的多重pcr方法及其应用
CN101821619A (zh) * 2007-09-07 2010-09-01 弗卢丁公司 拷贝数变化确定、方法和系统

Also Published As

Publication number Publication date
US20210285035A1 (en) 2021-09-16
JP2018534942A (ja) 2018-11-29
EP3380634A1 (en) 2018-10-03
WO2017089767A1 (en) 2017-06-01

Similar Documents

Publication Publication Date Title
CN102203287B (zh) 增加样本和/或靶通量的测定方法
JP2023063359A (ja) ポリヌクレオチドバーコード生成
EP2569453B1 (en) Nucleic acid isolation methods
EP3174997B1 (en) Method for selecting a target nucleic acid sequence
US20100285537A1 (en) Selective tagging of short nucleic acid fragments and selective protection of target sequences from degradation
RU2609630C2 (ru) Геномный отбор и секвенирование с помощью кодированных микроносителей
US9422602B2 (en) Methods and compositions for determining nucleic acid degradation
CN107012202B (zh) 核酸扩增的精度管理方法、精度管理用试剂及其试剂盒
US20210246499A1 (en) Detection of short homopolymeric repeats
CN108699597A (zh) 单分子对照
JP2022110152A (ja) 診断方法及び組成物
EP3325152A1 (en) Automated sample to ngs library preparation
CN107250377A (zh) 生物样品中的核酸的扩增和检测
JP6202455B2 (ja) 標的核酸の検出方法
HK40082697A (en) Sample preparation for nucleic acid amplification
CN114728256A (zh) 通过非终点扩增进行的单细胞的靶标拷贝数的分区型确定
JP2007075023A (ja) 蛍光共鳴エネルギー移動法を用いた遺伝子多型検出法及びキット
HK1198661B (zh) 核酸编码反应

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
WD01 Invention patent application deemed withdrawn after publication
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20181023