CN108699135B - 用于改善病毒载体效率的组合物和方法 - Google Patents
用于改善病毒载体效率的组合物和方法 Download PDFInfo
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- CN108699135B CN108699135B CN201680070996.0A CN201680070996A CN108699135B CN 108699135 B CN108699135 B CN 108699135B CN 201680070996 A CN201680070996 A CN 201680070996A CN 108699135 B CN108699135 B CN 108699135B
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/82—Translation products from oncogenes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
- A61K48/0008—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'non-active' part of the composition delivered, e.g. wherein such 'non-active' part is not delivered simultaneously with the 'active' part of the composition
- A61K48/0016—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'non-active' part of the composition delivered, e.g. wherein such 'non-active' part is not delivered simultaneously with the 'active' part of the composition wherein the nucleic acid is delivered as a 'naked' nucleic acid, i.e. not combined with an entity such as a cationic lipid
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/06—Antianaemics
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- C—CHEMISTRY; METALLURGY
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- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/033—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the internal surface of the plasma membrane, e.g. containing a myristoylation motif
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/10—Fusion polypeptide containing a localisation/targetting motif containing a tag for extracellular membrane crossing, e.g. TAT or VP22
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
- C12N2740/15041—Use of virus, viral particle or viral elements as a vector
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
- C12N2740/15041—Use of virus, viral particle or viral elements as a vector
- C12N2740/15043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14141—Use of virus, viral particle or viral elements as a vector
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14141—Use of virus, viral particle or viral elements as a vector
- C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14311—Parvovirus, e.g. minute virus of mice
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
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- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Immunology (AREA)
- Virology (AREA)
- Oncology (AREA)
- Diabetes (AREA)
- Hematology (AREA)
- Toxicology (AREA)
- Epidemiology (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP15306924.0 | 2015-12-03 | ||
| EP15306924 | 2015-12-03 | ||
| EP16194180 | 2016-10-17 | ||
| EP16194180.2 | 2016-10-17 | ||
| PCT/EP2016/079304 WO2017093330A1 (en) | 2015-12-03 | 2016-11-30 | Compositions and methods for improving viral vector efficiency |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108699135A CN108699135A (zh) | 2018-10-23 |
| CN108699135B true CN108699135B (zh) | 2022-10-04 |
Family
ID=57485463
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201680070996.0A Expired - Fee Related CN108699135B (zh) | 2015-12-03 | 2016-11-30 | 用于改善病毒载体效率的组合物和方法 |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US11371061B2 (enExample) |
| EP (1) | EP3383896A1 (enExample) |
| JP (2) | JP7350485B2 (enExample) |
| CN (1) | CN108699135B (enExample) |
| CA (1) | CA3004971A1 (enExample) |
| WO (1) | WO2017093330A1 (enExample) |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10596264B2 (en) * | 2011-06-30 | 2020-03-24 | Genethon | Peptides with viral infection enhancing properties and their use |
| WO2015191508A1 (en) | 2014-06-09 | 2015-12-17 | Voyager Therapeutics, Inc. | Chimeric capsids |
| CN107106689A (zh) | 2014-11-05 | 2017-08-29 | 沃雅戈治疗公司 | 用于治疗帕金森病的aadc多核苷酸 |
| KR102584655B1 (ko) | 2014-11-14 | 2023-10-06 | 보이저 테라퓨틱스, 인크. | 조절성 폴리뉴클레오티드 |
| MX2017006216A (es) | 2014-11-14 | 2018-08-29 | Voyager Therapeutics Inc | Composiciones y métodos para tratar la esclerosis lateral amiotrófica (ela). |
| US11697825B2 (en) | 2014-12-12 | 2023-07-11 | Voyager Therapeutics, Inc. | Compositions and methods for the production of scAAV |
| WO2017189964A2 (en) | 2016-04-29 | 2017-11-02 | Voyager Therapeutics, Inc. | Compositions for the treatment of disease |
| US11326182B2 (en) | 2016-04-29 | 2022-05-10 | Voyager Therapeutics, Inc. | Compositions for the treatment of disease |
| AU2017268382B2 (en) | 2016-05-18 | 2023-09-28 | Voyager Therapeutics, Inc. | Compositions and methods of treating Huntington's disease |
| RU2758488C2 (ru) | 2016-05-18 | 2021-10-28 | Вояджер Терапьютикс, Инк. | Модулирующие полинуклеотиды |
| JP2019531787A (ja) | 2016-08-30 | 2019-11-07 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | 生物医学的ターゲティング及びデリバリーの方法並びにそれを実行するための装置及びシステム |
| AU2018261790B2 (en) | 2017-05-05 | 2024-10-03 | Voyager Therapeutics, Inc. | Compositions and methods of treating amyotrophic lateral sclerosis (ALS) |
| WO2018204803A1 (en) | 2017-05-05 | 2018-11-08 | Voyager Therapeutics, Inc. | Compositions and methods of treating huntington's disease |
| JOP20190269A1 (ar) | 2017-06-15 | 2019-11-20 | Voyager Therapeutics Inc | بولي نوكليوتيدات aadc لعلاج مرض باركنسون |
| CN111132626B (zh) | 2017-07-17 | 2024-01-30 | 沃雅戈治疗公司 | 轨迹阵列引导系统 |
| EP3662060A2 (en) | 2017-08-03 | 2020-06-10 | Voyager Therapeutics, Inc. | Compositions and methods for delivery of aav |
| JP2020530463A (ja) * | 2017-08-10 | 2020-10-22 | アーディジェン, エルエルシー | ウイルスの細胞内送達のためのペプチドおよびナノ粒子 |
| CN107686523B (zh) * | 2017-09-15 | 2021-05-14 | 山西大学 | 一种肿瘤酸度响应自噬诱导多肽及其制备方法和应用 |
| EP3687582A4 (en) | 2017-09-29 | 2021-07-14 | Voyager Therapeutics, Inc. | RESCUE OF CENTRAL AND PERIPHERAL NEUROLOGICAL PHENOTYPE OF FRIEDREICH ATAXIA BY INTRAVENOUS ADMINISTRATION |
| EP4454654A3 (en) | 2017-10-16 | 2025-02-19 | Voyager Therapeutics, Inc. | Treatment of amyotrophic lateral sclerosis (als) |
| US11434502B2 (en) | 2017-10-16 | 2022-09-06 | Voyager Therapeutics, Inc. | Treatment of amyotrophic lateral sclerosis (ALS) |
| MX2020012077A (es) | 2018-05-15 | 2021-03-09 | Voyager Therapeutics Inc | Composiciones y metodos para el tratamiento de la enfermedad de parkinson. |
| SG11202103151RA (en) | 2018-09-28 | 2021-04-29 | Voyager Therapeutics Inc | Frataxin expression constructs having engineered promoters and methods of use thereof |
| JP7394493B2 (ja) * | 2019-07-31 | 2023-12-13 | レモネックス インコーポレイテッド | 抗癌剤および多孔性シリカ粒子の製造方法 |
| WO2022046800A2 (en) * | 2020-08-24 | 2022-03-03 | University Of Southern California | Beclin 2 and uses thereof for treating cancer and neurodegenerative diseases |
| EP4291666A4 (en) * | 2021-02-15 | 2025-07-09 | Biomarin Pharm Inc | USE OF HISTIDINE-RICH PEPTIDES AS TRANSFECTION REAGENTS FOR THE PRODUCTION OF RAAV AND RBV |
| CN113713082B (zh) * | 2021-08-27 | 2023-07-18 | 福建医科大学 | 一种用于阿尔茨海默症的纳米自噬诱导剂及其制备方法与应用 |
| CN114316065B (zh) * | 2021-10-29 | 2023-09-22 | 新乡医学院 | 小分子肽及其应用、细胞培养基、核酸分子 |
| WO2024243639A1 (en) * | 2023-05-31 | 2024-12-05 | The Florey Institute Of Neuroscience And Mental Health | Autophagy-inducing peptides |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011106684A2 (en) * | 2010-02-25 | 2011-09-01 | San Diego State University Foundation | Compositions and methods for modulating autophagy |
| WO2013119377A1 (en) * | 2012-02-11 | 2013-08-15 | Board Of Regents, The University Of Texas System | Autophagy-inducing peptide |
| CN104080799A (zh) * | 2011-06-30 | 2014-10-01 | 吉尼松公司 | 具有病毒感染增强性质的肽及其用途 |
| WO2015042272A1 (en) * | 2013-09-18 | 2015-03-26 | Ndsu Research Foundation | COMPOSITIONS AND METHODS FOR TREATING CONDITIONS ASSOCIATED WITH γ-HERPESVIRUSES |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7083979B1 (en) | 1994-03-25 | 2006-08-01 | Indiana University Foundation | Methods for enhanced retroviral-mediated gene transfer |
| WO2014109728A1 (en) * | 2013-01-11 | 2014-07-17 | The Scripps Research Institute | Methods and compositions for enchancing transduction efficiency of retroviral vectors |
| US20140271584A1 (en) | 2013-03-15 | 2014-09-18 | The Research Foundation For The State University Of New York | Methods and Compositions for Direct Reprogramming of Somatic Cells to Stem Cells, and Uses of these Cells |
| WO2016119856A1 (en) * | 2015-01-29 | 2016-08-04 | Universite De Strasbourg | Autophagy-inducing molecules for increasing insulin release |
| EP3141680B1 (en) * | 2015-09-09 | 2018-01-31 | U-Shin Italia S.p.A. | Electronic handle for a vehicle door |
| US20200230207A1 (en) * | 2015-09-28 | 2020-07-23 | Fondazione Telethon | Treatment of bone growth disorders |
| WO2017161274A1 (en) * | 2016-03-18 | 2017-09-21 | Trustees Of Tufts College | Cyclic peptide epitopes and small-molecule mimics for inducing autophagy |
| WO2018013568A1 (en) * | 2016-07-11 | 2018-01-18 | University Of North Texas Health Science Center At Fort Worth | Treatment for glaucoma and other eye diseases |
| WO2018222723A1 (en) * | 2017-05-30 | 2018-12-06 | Vertex Pharmaceuticals Incorporated | C3 fusion protein and methods of making and using thereof |
-
2016
- 2016-11-30 EP EP16806032.5A patent/EP3383896A1/en not_active Withdrawn
- 2016-11-30 WO PCT/EP2016/079304 patent/WO2017093330A1/en not_active Ceased
- 2016-11-30 US US15/772,902 patent/US11371061B2/en active Active
- 2016-11-30 JP JP2018528340A patent/JP7350485B2/ja active Active
- 2016-11-30 CA CA3004971A patent/CA3004971A1/en active Pending
- 2016-11-30 CN CN201680070996.0A patent/CN108699135B/zh not_active Expired - Fee Related
-
2021
- 2021-10-01 JP JP2021162777A patent/JP2022002530A/ja active Pending
-
2022
- 2022-05-23 US US17/750,705 patent/US20230117384A1/en not_active Abandoned
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011106684A2 (en) * | 2010-02-25 | 2011-09-01 | San Diego State University Foundation | Compositions and methods for modulating autophagy |
| CN104080799A (zh) * | 2011-06-30 | 2014-10-01 | 吉尼松公司 | 具有病毒感染增强性质的肽及其用途 |
| WO2013119377A1 (en) * | 2012-02-11 | 2013-08-15 | Board Of Regents, The University Of Texas System | Autophagy-inducing peptide |
| WO2015042272A1 (en) * | 2013-09-18 | 2015-03-26 | Ndsu Research Foundation | COMPOSITIONS AND METHODS FOR TREATING CONDITIONS ASSOCIATED WITH γ-HERPESVIRUSES |
Non-Patent Citations (4)
| Title |
|---|
| Beclin 2 functions in autophagy, degradation of G protein-coupled receptors, and metabolism;He C,等;《Cell》;20130829;第154卷(第5期);第1085-1099页 * |
| Common and divergent functions of Beclin 1 and Beclin 2;Galluzzi L,等;《Cell Res》;20131231;第23卷(第12期);第一段 * |
| Identification of a candidate therapeutic autophagy-inducing peptide;Shoji-Kawata S 等;《Nature》;20120214;第94卷(第7436期);摘要 * |
| Peptides derived from evolutionarily conserved domains in Beclin-1 and Beclin-2 enhance the entry of lentiviral vectors into human cells;Majdoul Saliha等;《JOURNAL OF BIOLOGICAL CHEMISTRY》;20171110;第292卷(第45期);第18672-18681页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| US20230117384A1 (en) | 2023-04-20 |
| CA3004971A1 (en) | 2017-06-08 |
| JP2019501882A (ja) | 2019-01-24 |
| JP7350485B2 (ja) | 2023-09-26 |
| CN108699135A (zh) | 2018-10-23 |
| EP3383896A1 (en) | 2018-10-10 |
| US20180318447A1 (en) | 2018-11-08 |
| JP2022002530A (ja) | 2022-01-11 |
| WO2017093330A1 (en) | 2017-06-08 |
| US11371061B2 (en) | 2022-06-28 |
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