CN108684717B - Attractant containing yeast extract and preparation method thereof - Google Patents
Attractant containing yeast extract and preparation method thereof Download PDFInfo
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- CN108684717B CN108684717B CN201810819314.2A CN201810819314A CN108684717B CN 108684717 B CN108684717 B CN 108684717B CN 201810819314 A CN201810819314 A CN 201810819314A CN 108684717 B CN108684717 B CN 108684717B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/18—Vapour or smoke emitting compositions with delayed or sustained release
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/54—1,3-Diazines; Hydrogenated 1,3-diazines
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having no bond to a nitrogen atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N51/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds having the sequences of atoms O—N—S, X—O—S, N—N—S, O—N—N or O-halogen, regardless of the number of bonds each atom has and with no atom of these sequences forming part of a heterocyclic ring
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
- A01N57/10—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds
- A01N57/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing heterocyclic radicals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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Abstract
The invention relates to the technical field of daily insecticidal products, in particular to an attractant containing a yeast extract and a preparation method thereof. According to the invention, the yeast extract is added into the attractant, the moisture and the form in the attractant can be kept by utilizing the yeast extract, the addition of a humectant and saccharides is not required, in addition, the attraction performance of the attractant on insects, especially cockroaches, can be improved by utilizing the specific odor characteristic of the yeast extract, and in addition, the problems of unstable performances of the attractant product, such as form liquefaction, package bulging, odor change and the like caused by the addition of active yeast in the prior art can be overcome by adding the yeast extract. According to the invention, by adding the insect powder component into the attractant and utilizing the sex pheromone and the aggregation pheromone contained in the insect body, the attractant can generate an attracting aggregation effect on cockroaches, flies and ants, and the attracting performance of the attractant can be further improved. The attractant disclosed by the invention can balance the smell among the components, so that the attractant has better attraction performance and a remarkable trapping and killing effect.
Description
Technical Field
The invention relates to the technical field of daily insecticidal products, in particular to an attractant containing a yeast extract and a preparation method thereof.
Background
The environmental sanitation of home furnishing and other moving places is maintained, and the prevention and the killing of harmful insects such as cockroaches, flies, ants and the like are the problems which need to be considered. Especially cockroaches, which are widely present in various regions in China, and common varieties include periplaneta americana, german cockroach, australian cockroach, black chest cockroach and the like; cockroaches inhabit in dark and hidden humid spaces due to complicated feeding habits, emergence in the daytime and at night, and favors to inhabit and have strong reproductive capacity, and are superposed with the current conditions of accelerated commodity circulation and dense houses, so that the cockroaches become common sanitary pests in families, business supermans, leisure places, public service institutions and the like; the construction of an peaceful healthy living environment by killing cockroaches has become a common demand for people.
At present, pest control products, such as cockroach killing aerosol, cockroach killing aerosol and cockroach killing incense in cockroach control products, kill cockroaches in a mode of medicament spraying retention or fumigating and killing, and pollute the air quality of the use environment to different degrees. The attractant containing the insecticidal component can attract cockroaches to eat and poison the cockroaches to achieve a precise and targeted cockroach killing effect, and is more environment-friendly compared with cockroach killing aerosol and cockroach killing smoke agent products. Compared with the insect aerosol and the fly incense, the attractant containing the insecticidal component has obvious advantages of safety and environmental protection, and can attract flies to lick and kill the flies, and meanwhile, the attractant containing the insecticidal component can enable ants and other insects to carry the attractant to return to the nest to realize integral killing.
Chinese patent document CN 105340976 a discloses an attractant for cockroaches containing active yeast, which is prepared by mixing sugar and grains, adding the mixture into active yeast, fermenting for 1-8 months, wherein the originally added components and material forms will change, and the disadvantage is not favorable for application and popularization of the attractant. Chinese patent document CN 106818840A also discloses a cockroach bait containing an active yeast component and a preparation method thereof, and the prepared cockroach bait product consumes components therein due to yeast fermentation to generate ethanol, water and carbon dioxide, thereby causing liquefaction of product form, swelling of package, odor change and the like. The techniques disclosed in both of the above patent documents consume the remaining components in the product by adding active yeast, so that the design intent thereof cannot be embodied in practice. Chinese patent document CN 105660725 a discloses a powder for attracting cockroaches, which has simple components and weak attraction.
The cockroach trapping and killing products on the market do not contain yeast extract components, and the attractant has relatively single component, weak component attraction and poor cockroach killing effect.
Disclosure of Invention
Aiming at the problems of single components, weak component attraction and further improvement of killing effect of the existing pest control product, the invention provides the attractant for trapping and killing pests, which can improve the attraction capacity by adding the yeast extract and does not need to additionally add saccharides and humectants, and the preparation method of the attractant.
In order to achieve the purpose, the invention adopts the following technical scheme.
An attractant containing yeast extract comprises the following components in percentage by mass, and the sum of the percentage by mass of the following components is 100%: 0.1-10% of yeast extract, 0.1-5% of insect powder, 30-70% of food attractant, 0.05-3% of insecticidal component, 0.01-1% of preservative, 1-10% of vegetable oil and fat and the balance of water.
Preferably, the insect powder is at least one of cockroach powder, fly maggot powder, silkworm chrysalis powder and ground beetle powder.
Preferably, the food material attractant is at least one of wheat flour, corn flour, vinasse powder, rice bran powder, sesame powder, beet powder and banana powder.
Preferably, the pesticide is fipronil, imidacloprid, hydramethylnon, chlorpyrifos or dinotefuran.
Preferably, the preservative is at least one of isothiazolinone, phenoxyethanol, sodium dehydroacetate, benzyl alcohol and triclocarban.
Preferably, the vegetable oil is at least one of sunflower seed oil, cottonseed oil, wheat germ oil and corn germ oil.
Preferably, the yeast extract is prepared from saccharomyces cerevisiae serving as a raw material.
The preparation method of the attractant containing the yeast extract comprises the following steps:
s1, mixing the insecticidal component and the vegetable oil and uniformly stirring to obtain a first mixture; the preservative is mixed with water and stirred uniformly to obtain a second mixture.
S2, uniformly mixing the yeast extract, the insect powder and the food material attractant, then adding the first mixture into the mixture, uniformly stirring, and then uniformly mixing the mixture and the second mixture to obtain the attractant.
After step S2, the attractant may be made into pellets, powder or granules according to the product design requirements, and then packaged into finished products.
The preparation method of the insect powder comprises the following steps: pulverizing the frozen insects with a pulverizer to obtain powder; and then heating the powder to dry and sterilize the powder to obtain the insect powder.
Specifically, the preparation method of the insect powder comprises the following steps: freezing insect body in a freezing chamber at-20 deg.C for 30min, pulverizing the insect body in a pulverizer, sieving with 80 mesh sieve, heating the obtained powder at 82-85 deg.C for 8min, sterilizing, and drying.
Wherein the preparation method of the yeast extract comprises the following steps: adding yeast into 2 wt.% of sodium chloride aqueous solution, and homogenizing to obtain a mixture; drying the mixture to obtain the yeast extract.
Specifically, the preparation method of the yeast extract comprises the following steps: adding yeast into 2 wt.% of sodium chloride aqueous solution, and homogenizing under 30MPa for 1h to obtain a mixture; drying the mixture to obtain the yeast extract.
Compared with the prior art, the invention has the beneficial effects that: according to the invention, the yeast extract component is added into the attractant, the glucan, the mannan and the polypeptide substance in the yeast extract are utilized to maintain the moisture and the form in the attractant, no additional humectant and saccharide are required to be added, in addition, the attraction performance of the attractant to insects, especially cockroaches can be improved by utilizing the specific odor characteristic of the yeast extract, and in addition, the problems of unstable performances of the attractant product, such as form liquefaction, package bulging, odor change and the like caused by adding active yeast in the prior art can be overcome by adding the yeast extract. According to the invention, by adding the insect powder component into the attractant and utilizing the sex pheromone and the aggregation pheromone contained in the insect body, the attractant can generate an attracting aggregation effect on cockroaches, flies and ants, and the attracting performance of the attractant can be further improved. According to the invention, the yeast extract and the insect powder are added into the attractant, and the dosage proportion of each component is optimized, so that the odor among the components can be balanced, the attractant has better attraction performance, especially excellent attraction performance for cockroaches, and has a remarkable trapping and killing effect. The yeast extract is prepared from saccharomyces cerevisiae serving as a raw material, so that yeast produced by a brewing enterprise is comprehensively applied, and the main components of the invention adopt raw materials with natural sources, so that the attractant for trapping and killing pests, which has the advantages of natural sources of the inducing raw materials, comprehensive application and superior trapping and killing performance, is constructed.
Drawings
FIG. 1 is a graph showing the ratio of yeast extract added to an attractant versus the amount of cockroach sticking to it;
FIG. 2 is a graph showing the ratio of yeast extract added to the attractant and the amount consumed by the amount of cockroach ingested.
Detailed Description
In order to more fully understand the technical contents of the present invention, the technical solutions of the present invention will be further described and illustrated with reference to the following specific embodiments.
Examples 1 to 28
Examples 1-28 provide an attractant comprising a yeast extract and a method of making an attractant comprising a yeast extract, respectively.
In each example, the mass percentages of the components are shown in the following table 1, the attractant in each example comprises the components in the following table and water, and the sum of the mass percentages of the components and the water in the following table is 100%.
Table 1 each component and amount (wt.%) of the attractant in examples 1-28
The preparation method of the attractant containing the yeast extract described in examples 1-28 specifically comprises the following steps:
(1) preparing insect powder: freezing the insect body in a freezing chamber at the temperature of 20 ℃ below zero for 30min to freeze and harden the insect body, then placing the frozen insect body into a crusher to crush and sieving the crushed insect body with a sieve of 80 meshes, then flatly paving the obtained powder and placing the powder into a microwave dryer with the power of 1KW and the temperature of 82 ℃ to 85 ℃ to heat for 8min so as to sterilize and dry the powder, thus obtaining the insect powder. And (5) standby.
(2) Preparation of yeast extract: adding yeast into 2 wt.% of sodium chloride aqueous solution, then placing the liquid into a high-pressure homogenizer, and homogenizing for 1h under the pressure of 30MPa to obtain a mixture; drying the mixture to obtain the yeast extract. And (5) standby.
(3) Mixing the insecticidal component and the vegetable oil and uniformly stirring to obtain a first mixture; the preservative is mixed with water and stirred uniformly to obtain a second mixture.
(4) Uniformly mixing the yeast extract, the insect powder and the food attractant, then adding the first mixture into the mixture, uniformly stirring, and then uniformly mixing the mixture and the second mixture to obtain the attractant.
The attractant prepared by the above embodiments can be made into pills, powder or granules according to the design requirements of the product, and then packaged into finished products.
The attractants prepared in examples 1 to 28 were evaluated for luring properties by the following methods:
respectively placing the attractants prepared in the examples 1-27 into one corner of different insect (cockroach) feeding jars, simultaneously placing the attractants prepared in the example 28 with the same amount at the diagonal positions in the insect feeding jars, recording the weight loss data of the two attractants in the same time period (24h), and showing that the great sample weight loss indicates that the attractant has obvious feeding advantages on insects; the test results are shown in table 2 below.
A cockroach hiding box is placed in the center of a closed simulation site of 15 square meters and 28 cubic meters, 50 cockroaches are placed, a cockroach sticking plate is placed in one corner of the simulation site after the cockroaches move normally, the attractant of the embodiment is placed in the center of the cockroach sticking plate, the attractant prepared in the comparative example 28 is placed on the sticky plate in the diagonal position of the simulation site, a video is recorded by a camera in the testing process, and the attractiveness is evaluated according to the number of insects which are stuck or gathered on each sample. The test results are shown in table 3 below.
The results of the tests of the attractants prepared in examples 1 to 16 are shown in FIG. 1, which is a graph of the ratio of the yeast extract added to the cockroach-sticking cockroach, and in FIG. 2, which is a graph of the ratio of the yeast extract added to the cockroach-eating amount consumed.
Table 2 feeding performance test results of the attractants prepared in examples 1 to 28 for cockroaches
Table 3 attraction performance test results of the attractants prepared in examples 1 to 28 for cockroaches
Evaluation example 11 and example 28, after preparing the attractant into granular attractant, the granular attractant was placed in a culture dish and placed in an ant feeding jar, and the attractant carried out of the culture dish was taken into account in the consumption amount because ants like to eat and carry, and the preference of ants was directly characterized by the consumption amount.
Table 4 test results of the preference of the attractants prepared in examples 11 and 28 for ants
| Examples | Example 11 | Example 28 |
| Consumption (gram) | 7 | 2 |
Evaluation of attractants of example 21 and example 27 were prepared as pellet attractants, the pellet attractants were placed in the center of a fly sticking board, 100 flies were placed in a simulation site of 15 square meters and 28 cubic meters, the pellet attractant sticking board of example 21 was placed in one corner of the simulation site, the pellet attractant sticking board of example 27 was placed in the diagonal of the pellet attractant sticking board, and the number of fly sticking on each sticking board was recorded after 24 hours; the test results are shown in Table 5.
Table 5 results of attraction test of attractants prepared in example 21 and example 27 to flies
| Examples | Example 21 | Example 27 |
| Quantity of sticking catches (only) | 52 | 29 |
Evaluation of attractants of example 21 and example 27 were prepared as pellet attractants, the pellet attractants were placed in petri dishes separately, 100 flies were placed in two 15 square meter, 28 cubic meter simulation sites, the pellet attractant of example 21 was placed in one corner of one simulation site, the pellet attractant of example 27 was placed in one corner of the other simulation site, and a cotton ball of fructose in water solution was placed in a diagonal position of the pellet attractants in the two simulation sites; fly mortality was recorded every 24 hours; the test results are shown in Table 6. Insect powder of the different examples was characterized by its feeding preference for fly attraction by mortality.
Table 6 mortality test results of the attractants prepared in example 21 and example 27 on flies
| Examples | Example 21 | Example 27 |
| Mortality rate% | 85 | 56 |
The technical contents of the present invention are further illustrated by the examples, so as to facilitate the understanding of the reader, but the embodiments of the present invention are not limited thereto, and any technical extension or re-creation based on the present invention is protected by the present invention.
Claims (8)
1. The attractant containing the yeast extract is characterized by comprising the following components in percentage by mass, and the sum of the percentage by mass of the following components is 100%: 10% of yeast extract, 2.5% of insect powder, 30-70% of food attractant, 0.05-3% of insecticidal component, 0.01-1% of preservative, 1-10% of vegetable oil and fat and the balance of water;
the insect powder is at least one of cockroach powder, fly maggot powder, silkworm chrysalis powder and ground beetle powder;
the food material attractant is at least one of wheat flour, corn flour, vinasse powder, rice bran powder, sesame powder, beet powder and banana powder;
wherein the preparation method of the yeast extract comprises the following steps: adding yeast into a 2% sodium chloride aqueous solution by mass and homogenizing to obtain a mixture; drying the mixture to obtain the yeast extract.
2. An attractant containing yeast extract according to claim 1, wherein said insecticide is fipronil, imidacloprid, hydramethylnon, chlorpyrifos or dinotefuran.
3. An attractant containing yeast extract according to claim 1, wherein the preservative is at least one of isothiazolinone, phenoxyethanol, sodium dehydroacetate, benzyl alcohol, triclocarban.
4. An attractant containing yeast extract according to claim 1, wherein the vegetable oil is at least one of sunflower seed oil, cottonseed oil, wheat germ oil and corn germ oil.
5. The attractant containing the yeast extract as set forth in claim 1, wherein the yeast extract is prepared from saccharomyces cerevisiae.
6. A method for preparing an attractant containing yeast extract as claimed in claim 1, comprising the steps of:
s1, mixing the insecticidal component and the vegetable oil and uniformly stirring to obtain a first mixture; mixing the preservative with water and stirring uniformly to obtain a second mixture;
s2, uniformly mixing the yeast extract, the insect powder and the food material attractant, then adding the first mixture into the mixture, uniformly stirring, and then uniformly mixing the mixture and the second mixture to obtain the attractant.
7. The method for preparing the attractant containing the yeast extract as set forth in claim 6, wherein the insect powder is prepared by the following steps: pulverizing the frozen insects with a pulverizer to obtain powder; and then heating the powder to dry and sterilize the powder to obtain the insect powder.
8. The method for preparing the attractant containing the yeast extract as set forth in claim 6, wherein the method for preparing the yeast extract comprises the following steps: adding yeast into a 2% sodium chloride aqueous solution by mass and homogenizing to obtain a mixture; drying the mixture to obtain the yeast extract.
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| CN111513083A (en) * | 2019-04-22 | 2020-08-11 | 泰山医学院 | Novel housefly specific long-acting bacterial attractant and preparation method thereof |
| CN110558342A (en) * | 2019-10-18 | 2019-12-13 | 广州超威生物科技有限公司 | Household fly bait composition and preparation method thereof |
| CN115281197B (en) * | 2022-08-17 | 2024-05-24 | 中百欧(北京)环境科技有限公司 | Fly attractant |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1283958A (en) * | 1997-11-26 | 2001-02-14 | 特拉普姆斯有限公司 | Insect trap |
| CN1376028A (en) * | 1999-09-22 | 2002-10-23 | 埃科莱布有限公司 | Water-based pest baits containing water sensitive insecticides |
| CN1484960A (en) * | 2003-08-08 | 2004-03-31 | 高俊华 | Cockroach killing bait agent |
| CN1985604A (en) * | 2005-12-20 | 2007-06-27 | 陈政忠 | Roach killing bait |
| CN101731202A (en) * | 2008-11-25 | 2010-06-16 | 李伟 | Method for processing and preparing granular fresh bait for killing cockroaches |
| CN104472527A (en) * | 2014-11-12 | 2015-04-01 | 南通功成精细化工有限公司 | Dinotefuran insecticide having insect inducing effect |
-
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Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1283958A (en) * | 1997-11-26 | 2001-02-14 | 特拉普姆斯有限公司 | Insect trap |
| CN1376028A (en) * | 1999-09-22 | 2002-10-23 | 埃科莱布有限公司 | Water-based pest baits containing water sensitive insecticides |
| CN1484960A (en) * | 2003-08-08 | 2004-03-31 | 高俊华 | Cockroach killing bait agent |
| CN1985604A (en) * | 2005-12-20 | 2007-06-27 | 陈政忠 | Roach killing bait |
| CN101731202A (en) * | 2008-11-25 | 2010-06-16 | 李伟 | Method for processing and preparing granular fresh bait for killing cockroaches |
| CN104472527A (en) * | 2014-11-12 | 2015-04-01 | 南通功成精细化工有限公司 | Dinotefuran insecticide having insect inducing effect |
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