CN108675454B - Method for controlling whole process of blue algae bloom development in large shallow lake - Google Patents
Method for controlling whole process of blue algae bloom development in large shallow lake Download PDFInfo
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- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/50—Treatment of water, waste water, or sewage by addition or application of a germicide or by oligodynamic treatment
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
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- E—FIXED CONSTRUCTIONS
- E02—HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
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- E02B15/00—Cleaning or keeping clear the surface of open water; Apparatus therefor
- E02B15/04—Devices for cleaning or keeping clear the surface of open water from oil or like floating materials by separating or removing these materials
- E02B15/08—Devices for reducing the polluted area with or without additional devices for removing the material
- E02B15/0814—Devices for reducing the polluted area with or without additional devices for removing the material with underwater curtains
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
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Abstract
The invention discloses a method for controlling the whole process of blue algae water bloom development in a large shallow lake, which divides the blue algae water bloom development into five stages of a blue algae wintering stage, a recovery stage, a rapid growth stage, a outbreak stage and a blue algae death stage, determines the spatial distribution characteristics of blue algae in the lake in different stages of blue algae water bloom development, and adopts different treatment modes aiming at different development stages of the blue algae water bloom. The method of the invention firstly provides a method for carrying out system prevention and control on the whole process of the occurrence of the cyanobacteria bloom, expands the prevention and control of the cyanobacteria to the whole year scale, overcomes the current situation that the existing cyanobacteria prevention and control only aims at the little effect of the cyanobacteria bloom in summer, and can effectively reduce the occurrence intensity and probability of the cyanobacteria bloom in the whole year process.
Description
Technical Field
The invention belongs to the technical fields of water environment treatment, cyanobacterial bloom control and the like in the fields of environmental science, environmental engineering and ecological engineering, and particularly relates to eutrophication treatment, cyanobacterial bloom control and ecological restoration technologies for large shallow lakes, reservoirs, water source areas of the lakes and gulfs.
Background
The existing large-scale shallow lake blue algae bloom control method is mainly characterized in that the blue algae biomass is large and the distribution area is wide during the occurrence of the blue algae bloom in summer, and a common emergency measure is salvage, including manual salvage and collection and disposal of the blue algae bloom by using a blue algae salvage ship, an algae salvage platform, an algae water separation station and the like, so that the blue algae amount which can be treated is very limited, and the current situation of high-biomass blue algae aggregation is difficult to effectively change. Chinese patent CN101456603B discloses a method for treating eutrophic reservoir water bloom, which combines water flow partition board, ecological floating island, physical shading and in-situ aeration technologies, and utilizes the water flow partition board to divide a light control separation area and an intensified purification area in a water intake, source water flows through the water flow partition board and then enters the light control separation area, harmful algae rapidly float and gather on the water surface layer in the area due to lack of illumination, and are intercepted and trapped by the plant root system of the floating island, thus having a certain effect on reducing the water bloom blue algae, but the method does not relate to the harvesting and disposal of the water bloom blue algae, and the area of the water area of the effect is relatively limited. Chinese patent CN100519442C discloses a controllable method for rapidly eliminating cyanobacterial bloom in a reservoir, which comprises the steps of putting filter-feeding fishes, snails, river crabs, corbicula fluminea, mussels and other water body benthonic animals, and regulating and controlling water level; on the basis of adjustable water area, the invention takes long-term prevention and short-term treatment of filter-feeding fish as principle concepts, adopts a means that benthonic organisms eliminate blue-green algae (microcystis and anabaena) dormant bodies as main parts and filter-feeding fish control the early-stage generation density of the blue-green algae in the water body, and designs a way for benthonic animals to degrade the excrement of the filter-feeding fish. However, the invention explains the principle of various technical measures aiming at small lake and reservoir water bodies (the area is below 5000 mu), does not explain time nodes for controlling and eliminating blue algae in each technical link in detail, only proposes to put fish fries in 1-2 months, and does not discriminate key parameters and threshold values of different stages of the whole process of the water bloom of the blue algae, thereby adopting a targeted algae control technology according to the whole-process growth rule of the blue algae and the space-time distribution pattern of the water bloom blue algae. Chinese patent CN1562809A discloses a method for controlling cyanobacterial bloom, which comprises the steps of breeding filter-feeding silver carp, bighead carp and crucian carp in a water bloom outbreak area to play a role in controlling algae; secondly, zooplankton feeds on the small algae, and the quantity of the algae can be reduced; thirdly, the snail and the mussel can carry the roe to move, so that the distribution area of the fish is enlarged; fourthly, after a large amount of phycophage, algicidal bacteria and other microorganisms swallowing algae are cultured in a laboratory, the phycophage, the algicidal bacteria and other microorganisms are thrown into a cyanobacterial bloom outbreak area to play a role in controlling algae; fifthly, the recovery of aquatic plants in the water bloom outbreak area can also play a role in controlling algae; sixthly, the mechanical algae removal device. The technology disclosed by the invention is a conceptual method mostly, and besides the total stocking amount of fishes and hyriopsis, the stocking proportion, the stocking and controlling density of zooplankton, the density of algae-eating microorganisms and the planting amount of aquatic plants are not described, so that the practical realization is difficult. And the cyanobacteria bloom is controlled by utilizing microorganisms such as cyanophage and algicidal bacteria, such as CN102134126B, CN102206605B, CN102352326B, CN103509744B and CN103509745B, so that the cyanobacteria bloom plays a good role in controlling algae in small water bodies and landscape water bodies, but the cyanobacteria bloom has risks and is not ideal in large lakes.
Disclosure of Invention
The invention aims to provide a method for controlling the whole process of the development of blue algae water bloom in a large shallow lake.
In order to achieve the purpose, the development overall process of the cyanobacterial bloom is divided into five stages based on the annual growth rule of the cyanobacterial in the large shallow lake, and specific algae prevention and control measures are taken in different stages, wherein the measures comprise reduction of the settlement amount of the cyanobacterial decay period, the total bottom sediment cyanobacterial storage amount, the cyanobacterial recovery amount, the growth rate after the cyanobacterial recovery, the cyanobacterial bloom biomass and the like, so that the generation frequency and the area of the cyanobacterial bloom in summer are reduced, and the effective prevention and control of the cyanobacterial bloom in the overall process all the year around is realized.
The method of the invention is concretely as follows:
dividing the development of the cyanobacterial bloom into five stages of a cyanobacterial overwintering period, a recovery period, a rapid growth period, an outbreak period and a cyanobacterial decay period, determining the spatial distribution characteristics of the cyanobacterial in lakes at different stages of the cyanobacterial bloom development, and adopting different treatment modes aiming at different development stages of the cyanobacterial bloom.
The overwintering period and the recovery period of the blue algae are the early growth period of the blue algae, and the stock of blue algae sources is reduced and algae is inhibited by adopting bottom mud elution in-situ replacement, bottom mud nitrogen and phosphorus in-situ passivation, bottom mud covering and ecological dredging, so that the blue algae sources are removed.
The method is characterized in that the number of algae cells is reduced by controlling the algae cells in the recovery period and the rapid growth period of the blue algae, and comprises the steps of killing algae by adopting straw leachate or hydrogen peroxide, removing algae and controlling algae in modified soil, and treating the large-area algal blooms by utilizing mechanized spraying equipment; meanwhile, an algal bloom generation area is early warned through lake monitoring data in the rapid growth period of the blue algae, and a floating and sinking type enclosure device is constructed in the early warning area to block and block algae; monitoring early warning and surrounding boundary in a rapid growth period form an observation-early warning-boundary intelligent integrated blue algae defense system, excessive accumulation of blue algae in a local sensitive bay area is avoided, the number of algae cells is reduced by adopting the means, and the massive proliferation of the blue algae cells is controlled.
In the blue algae outbreak period, according to the space distribution characteristics of blue algae water blooms, a floating and sinking type enclosure device is adopted to block algae and keep algae in large water bodies of lakes and coastal zones, the enclosure diversion is adopted to enrich the blue algae water blooms, and mechanical algae removal equipment and a blue algae enrichment and concentration device are utilized to carry out algae fishing and algae removal in an algae enrichment water area and remove the algae on the water surface; and biological algae control is carried out by combining with the filter feeding fish culture net cage. The physical biological algae removal technology is comprehensively adopted for preventing and removing algae, and on one hand, the floating type barricade is adopted for blocking which can adapt to the variable-amplitude annual fluctuation water level (2-8 m) of a large shallow lake, can resist large wind wave impact, has high-efficiency algae blocking effect and is convenient to implement and manage, and the cyanobacterial bloom is prevented from drifting to a protection area and a sensitive area; on the other hand, the water bloom of the blue algae is enriched by adopting the enclosure diversion, and the algae fishing and algae removing are carried out in the algae enrichment water area by adopting mechanical algae removing equipment and a mobile blue algae enrichment and concentration platform to remove the blue algae on the water surface; moreover, biological algae control is carried out by utilizing the silver carp culture net cage, so that the algae control effect is improved.
In the decline period of the blue algae, physical organisms are adopted to remove the algae, filter feeding fish culture net cages are utilized to control the algae, large-scale benthonic animals are utilized to control the algae, the blue algae enrichment and concentration device and modified soil are utilized to remove the algae, the biomass of the blue algae in the water body is reduced, the number of active cells from the lower layer of the blue algae to the surface of bottom mud is reduced, namely, the physical organisms algae removal technology is adopted to reduce the existing amount of the blue algae in the water body.
Wherein the stage division mode of the cyanobacterial bloom is as follows:
defining the overwintering period and the recovery period of the blue algae by adopting a ratio of the concentration of the phycocyanin in the water column to the concentration of the chlorophyll, measuring the concentration of the phycocyanin in the water column of the lake water body and the concentration of the chlorophyll in the period from october to april of the next year, and judging the first day of the overwintering period of the blue algae in the earliest day when the ratio of the concentration of the phycocyanin in the water column to the concentration of the chlorophyll is less than; after the blue-green algae overwinter, determining the earliest day of the ratio of the phycocyanin concentration in the water column to the chlorophyll concentration greater than 1, and determining the day as the first day of the recovery period of the blue-green algae;
core protein based on blue algae photosynthetic system I in blue algae rapid growth periodpsaDefining the relative expression quantity of the C gene, and determining lake water columns from April to SeptemberpsaRelative expression of the C gene during April to SeptemberpsaWhen the relative expression of the C gene reaches 0.6 for the first time, judging that the blue algae enters a rapid growth period;psathe relative expression level of the C gene was obtained as follows:
collecting water sample, collecting algae, and determining core protein of photosynthetic system I by using the following primerspsaCThe gene expression level of (3):
F-5’-CGCACGGAAGACTGTATTGG-3’，
R-5’-CATACTGCGGGTGGTTTCTGCTC-3’；
simultaneously, the same determination is carried out on the indoor culture microcystis to obtain the field in-situ algaepsaCGene expression level and indoor culture of microcystispsaCThe relative ratio of the gene expression amounts is the relative gene expression amount.
The outbreak period of the blue algae is defined based on the wind speed, the blue algae floats upwards and gathers to form a water bloom when the wind speed is less than 3.1m/s for the first time in summer, and the development of the water bloom of the blue algae is judged to start to enter the outbreak period;
the blue algae death period is defined by autumn cell death rate, the outbreak period is developed to autumn, and the cell death rate reaches 0.7 d for the first time-1Then, the blue algae is judged to enter the decline period.
The method divides the development of the cyanobacteria bloom into five stages based on the annual growth rule of the cyanobacteria and through key indexes and threshold values, and takes targeted algae control measures according to the growth characteristics and distribution rules of the cyanobacteria in different stages, thereby reducing the biomass of the cyanobacteria and reducing the occurrence frequency and scale of the cyanobacteria bloom. The method of the invention expands the prevention and control of the blue algae to the whole year scale, overcomes the current situation that the existing blue algae prevention and control only aims at the blue algae water bloom in summer and has little effect, and can effectively reduce the occurrence intensity and probability of the blue algae water bloom in the whole year process.
Drawings
FIG. 1 shows the annual change in the rate of blue algae cell death in the nido lake;
FIG. 2 is a graph of the annual variation of the phycocyanin/chlorophyll ratio in the water column of the nested lake;
FIG. 3 blue algae in the lake of nidopsaThe annual change in the relative expression level of the C gene.
Detailed Description
The embodiment takes the nested lake as an example, and the technical scheme of the invention is further explained by combining the description of the attached drawings.
The method for controlling the whole process of the development of the cyanobacterial bloom in the large-scale shallow lake determines key physiological and biochemical indexes and threshold values of different stages of the whole process of the cyanobacterial bloom through field investigation and indoor experiments; determining a space-time distribution rule of the blue algae in the sediment and the water body by combining field investigation and MODIS satellite remote sensing images; corresponding algae control measures are taken aiming at the blue algae in different stages to reduce the biomass, thereby reducing the frequency and the intensity of the occurrence of the blue algae bloom.
The method comprises the following specific steps:
through field annual investigation and monitoring, determining threshold values of different stages of the whole process of the cyanobacterial bloom by adopting key physiological and biochemical indexes, dividing the development of the cyanobacterial bloom into five stages, namely a cyanobacterial wintering stage, a revival stage, a rapid growth stage, a outbreak stage and a cyanobacterial decay stage, determining the spatial distribution characteristics of the cyanobacterial in lakes at different stages of the development of the cyanobacterial bloom, and adopting different treatment modes aiming at different development stages of the cyanobacterial bloom;
the overwintering period and the recovery period of the blue algae are as follows:
and in 9 months, when the blue algae enters the death period, the dead blue algae can be settled into the lake sediment, the number of the blue algae in the water body can be greatly reduced, the ratio of the water column phycocyanin concentration to the chlorophyll concentration is selected as a division threshold value of the wintering period and the recovery period, from 10 months to 4 months of the next year, the measured ratio of the water column phycocyanin concentration to the chlorophyll concentration is less than 1, the wintering period of the blue algae is determined, and when the ratio is more than 1, the recovery period of the blue algae is determined. In the spatial distribution, in the overwintering period of the blue-green algae, determining the total amount distribution pattern of the blue-green algae sources in the brooch lake, the distribution depth of the bottom mud active blue-green algae and the overwintering survival rate by adopting the sedimentation amount of blue-green algae debris and the residual accumulation amount of the bottom mud blue-green algae on the surface layer; and in the spring recovery period, determining the recovery amount and determining the spatial distribution characteristics of the recovery of the blue-green algae in the lake nest.
The overwintering period and the recovery period of the blue algae are the early growth period of the blue algae, and the stock reduction and algae inhibition of the blue algae are carried out by adopting bottom mud elution in-situ replacement, bottom mud nitrogen and phosphorus in-situ passivation, bottom mud covering and ecological dredging; in this embodiment, the bottom mud treatment is adopted to reduce the stock of the blue algae source and inhibit the algae, which specifically comprises: adding 40% FeCl3And CaCO3Injecting into surface layer bottom mud to make Fe3+Reach 300g/m2About 50% Ca (NO) will be added3)2Injecting into surface layer substrate to make NO3N is up to 10-12 g/m2And controlling the release of the phosphorus in the bottom mud.
The blue algae growth period is as follows:
collecting water sample, collecting algae, and determining core protein of photosynthetic system IpsaCThe amount of gene expression, and the use of the amount of gene expressionpsaCThe primers are as follows:
F-5’-CGCACGGAAGACTGTATTGG-3’；
R-5’-CATACTGCGGG TGGTTTC TGCTC-3’。
simultaneously, the microcystis with the best indoor culture condition is subjected to the same determination to obtain the field in-situ algaepsaCMicrocystis with gene expression and indoor optimum culture conditionpsaCRelative ratio of gene expression levels. Due to low temperature, the blue algae is cultivated in the field in winterpsaCThe relative expression amount of the gene is at a low level, and in spring during the 4 th monthpsaCThe relative expression quantity of the gene is rapidly increased, when the relative expression quantity reaches 0.6, the blue algae is determined to enter a rapid growth period, and thenpsaCThe relative expression of the gene reaches peak values in 5 months and 6 months, the gene begins to decline after 7 months, the blue algae enters the decay period after 10 months,psaCthe relative expression level of the gene was reduced to winter level (FIG. 3). In spatial distribution, in spring and summer, during rapid growth and outbreak period, the floating, drifting and accumulation of blue-green algae in the lakes and the intensity (frequency and area) of water blooms are analyzed by utilizing the in-situ growth rates and weather leading factors of the blue-green algae in different lake regionsAnd duration) to determine the spatial distribution of blue-green algae in the lake.
The method is characterized in that the number of algae cells is reduced by controlling the algae cells in the recovery period and the rapid growth period of the blue algae, and comprises the steps of killing algae by adopting straw leachate or hydrogen peroxide, removing algae and controlling algae in modified soil, and treating the large-area algal blooms by utilizing mechanized spraying equipment; meanwhile, an algal bloom generation area is early warned through lake monitoring data in the rapid growth period of the blue algae, and a floating and sinking type enclosure device is constructed in the early warning area to block and block algae.
This example uses H at a concentration of 10-15mg/L2O2And (3) killing algae, and then adding 15-30mg/L of chitosan modified soil into a water body to adsorb and settle algae cells.
The floating barricade device comprises a floating body, a skirt body and a balancing weight; the upper end of the skirt body is connected with the floating body, and the lower end of the skirt body is connected with the balancing weight to form the flexible enclosure, so that a water area needing to be protected and controlled is sealed; the balancing weight is a gabion or a salon, and the balancing weight is buried in bottom mud.
Blue algae outbreak period:
the blue algae water bloom outbreak period, the floating and gathering of the blue algae are mainly influenced by the wind speed, 3.1m/s is taken as an outbreak period division threshold, when the wind speed is more than 3.1m/s, the blue algae are mainly distributed in a water column below 0.5m of the water surface, and when the wind speed is less than 3.1m/s, most of the blue algae float on the water surface of the surface layer to form the blue algae water bloom.
In the blue algae outbreak period, according to the space distribution characteristics of blue algae water blooms, a floating and sinking type enclosure device is adopted to block algae and keep algae in large water bodies of lakes and coastal zones, the enclosure diversion is adopted to enrich the blue algae water blooms, and mechanical algae removal equipment and a blue algae enrichment and concentration device are utilized to carry out algae fishing and algae removal in an algae enrichment water area and remove the algae on the water surface; biological algae control is carried out by combining the filter feeding fish culture net cage; the filter feeding fish culture net cage of the embodiment selects silver carps for culture, and the culture density is controlled to be 100-plus-150 g/m3。
Blue algae death phase:
collecting water samples monthly in different lake regions, and determining the activity of the granular esterase and the soluble esterase and the decay of the soluble esteraseThe decay rate of the algae was counted. At a cell decay rate of 0.7 d-1In order to define the threshold value of the blue algae decay period, the blue algae cell decay rate is lowest in 1 month due to low temperature, then the blue algae cell decay rate is increased and is at a fluctuation level in 2-6 months, the water bloom is relatively serious in summer in 7-8 months, the life activity of the blue algae is strong, and the cell decay rate is relatively reduced. The blue algae begins to die along with the reduction of the temperature in 9 months, the cell die rate reaches the peak value in 10 months, and all three lake regions reach 0.7 d-1(figure 1), judging that the blue algae enters the decline period; in the aspect of spatial distribution, the blue algae biomass in the water body and the sediment is measured, the sinking time, the location and the flux of the blue algae in the lake are analyzed, and the space-time distribution characteristics of the blue algae in the lake in each main stage of the water bloom development are analyzed.
Physical organisms are adopted to remove algae in the blue algae death period, and filter feeding fish culture net cages are utilized to control algae, large-scale benthonic animals are utilized to control algae, a blue algae enrichment and concentration device and modified soil are utilized to remove algae, so that the blue algae biomass in a water body is reduced, and the number of active cells from the lower layer of the blue algae to the surface of bottom mud is reduced. In the embodiment, the physical organisms are adopted for removing algae, the algae removal comprises algae control of filter-feeding fish culture net cages and algae control of large benthonic animals, the filter-feeding fish culture net cages are silver carps, the culture density is controlled to be 100-plus 150g/m3(ii) a The large benthonic animals are snails or mussels, the snails are round river snails, and the throwing density is 200-2(ii) a The mussels are selected from anodonta woodiana, Cristaria plicata or hyriopsis cumingii, and the culture density is controlled at 400g/m2。
By adopting the method of the invention to comprehensively treat the cyanobacterial bloom, the outbreak frequency and the area reduction rate of the cyanobacterial bloom in the water area of the comprehensive demonstration engineering area of the lakeside zone of the western nestle lake combined fertilizer new city zone can reach more than 50 percent, the biomass of algae can be reduced by more than 40 percent, and the comprehensive index of water eutrophication is less than 60.
Claims (7)
1. A method for controlling the whole process of the development of blue algae bloom in a large shallow lake is characterized by comprising the following steps:
dividing the development of the cyanobacterial bloom into five stages of a cyanobacterial overwintering period, a recovery period, a rapid growth period, an outbreak period and a cyanobacterial decay period, determining the spatial distribution characteristics of the cyanobacterial in lakes at different stages of the development of the cyanobacterial bloom, and adopting different treatment modes aiming at different development stages of the cyanobacterial bloom;
defining the overwintering period and the recovery period of the blue algae by adopting a ratio of the phycocyanin concentration in the water column to the chlorophyll concentration, measuring the phycocyanin concentration and the chlorophyll concentration in the water column of the lake water body in the period from october to april of the next year, and determining that the blue algae enters the overwintering period when the ratio of the phycocyanin concentration in the water column to the chlorophyll concentration is less than 1; when the ratio of the phycocyanin concentration of the water column to the chlorophyll concentration is more than 1, determining that the blue algae enters a recovery period; blue algae source stock reduction and algae inhibition are carried out through bottom mud treatment in the overwintering period and the recovery period of blue algae, and the method comprises the following steps: adding 40% FeCl3And CaCO3Injecting into surface layer bottom mud to make Fe3+Reach 300g/m2And then 50% Ca (NO)3)2Injecting into surface layer substrate to make NO3N is up to 10-12 g/m2Controlling the release of the phosphorus in the bottom mud;
core protein based on blue algae photosynthetic system I in blue algae rapid growth periodpsaDefining the relative expression quantity of the C gene, and determining lake water columns from April to SeptemberpsaRelative expression of C gene in core protein of blue algae photosynthetic system IpsaWhen the relative expression quantity of the C gene reaches 0.6, determining that the blue algae enters a rapid growth period; the method is characterized in that the number of algae cells is reduced by controlling the algae cells in the recovery period and the rapid growth period of the blue algae, and comprises the steps of killing algae by adopting straw leachate or hydrogen peroxide, removing algae and controlling algae in modified soil, and treating the large-area algal blooms by utilizing mechanized spraying equipment; meanwhile, an algal bloom generation area is early warned through lake monitoring data in the rapid growth period of the blue algae, and a floating and sinking type enclosure device is constructed in the early warning area to block and block algae;
the blue algae outbreak period is defined based on the wind speed, and when the wind speed is less than 3.1m/s in summer, the blue algae floats upwards and gathers to form water bloom and enters the blue algae outbreak period; in the blue algae outbreak period, according to the space distribution characteristics of blue algae water blooms, a floating and sinking type enclosure device is adopted to block algae and keep algae in large water bodies of lakes and coastal zones, the enclosure diversion is adopted to enrich the blue algae water blooms, and mechanical algae removal equipment and a blue algae enrichment and concentration device are utilized to carry out algae fishing and algae removal in an algae enrichment water area and remove the algae on the water surface; biological algae control is carried out by combining the filter feeding fish culture net cage;
the blue algae decay period is defined by the decay rate of cells in autumn, and the decay rate of cells in autumn reaches 0.7 d-1Determining that the blue algae enters the decline period; physical organisms are adopted to remove algae in the blue algae death period, and filter feeding fish culture net cages are utilized to control algae, large-scale benthonic animals are utilized to control algae, a blue algae enrichment and concentration device and modified soil are utilized to remove algae, so that the blue algae biomass in a water body is reduced, and the number of active cells from the lower layer of the blue algae to the surface of bottom mud is reduced.
2. The method of claim 1, wherein the step of applying the coating comprises applying a coating to the substratepsaThe relative expression level of the C gene was obtained as follows:
collecting water sample, collecting algae, and determining core protein of photosynthetic system I by using the following primerspsaCThe gene expression level of (3):
F-5’-CGCACGGAAGACTGTATTGG-3’，
R-5’-CATACTGCGGGTGGTTTCTGCTC-3’；
simultaneously, the same determination is carried out on the indoor culture microcystis to obtain the field in-situ algaepsaCGene expression level and indoor culture of microcystispsaCThe relative ratio of the gene expression amounts is the relative gene expression amount.
3. The method as claimed in claim 1, wherein the fast growth phase of cyanobacteria uses H with a concentration of 10-15mg/L2O2And (5) killing algae.
4. The method as claimed in claim 1, wherein the algae control is carried out by using modified soil in the fast growth period of the blue algae, and the chitosan modified soil of 15-30mg/L is added into the water body after the algae killing is carried out by using the straw leachate or hydrogen peroxide to adsorb and settle the algae cells.
5. The method as claimed in claim 1, wherein the biological algae control is carried out by combining the outbreak period of blue algae with filter-feeding fish culture net cages, the filter-feeding fish culture net cage is silver carp, and the culture density is controlled at 100-3。
6. The method as claimed in claim 1, wherein the physical organisms are used for removing algae in the decline period of the blue algae, including the control of algae in filter-feeding fish culture net cages and the control of algae in large benthonic animals, wherein the filter-feeding fish culture net cages are silver carps, the culture density is controlled at 100-3(ii) a The large benthonic animals are snails or mussels, the snails are round river snails, and the throwing density is 200-2(ii) a The mussels are selected from anodonta woodiana, Cristaria plicata or hyriopsis cumingii, and the culture density is controlled at 400g/m2。
7. The method of claim 1, wherein the floatable and floatable barricade comprises a float, a skirt, and a weight; the upper end of the skirt body is connected with the floating body, and the lower end of the skirt body is connected with the balancing weight to form a flexible enclosure, so that a water area needing to be protected and controlled is closed; the balancing weight is a gabion or a salon, and the balancing weight is buried in bottom mud.
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| CN108409047B (en) | 2018-04-16 | 2020-05-05 | 中国科学院南京地理与湖泊研究所 | Method for systematically controlling rapid proliferation of blue algae cells in lake spring |
| CN112028192B (en) * | 2020-08-24 | 2022-03-15 | 江南大学 | Method for treating cyanobacterial bloom by combining green film, disturbance and zinc elements |
| CN112062236A (en) * | 2020-09-08 | 2020-12-11 | 中国科学院南京地理与湖泊研究所 | In-situ cleaning method for overwintering blue algae seed source |
| CN113516373B (en) * | 2021-06-18 | 2023-07-25 | 中国科学院南京地理与湖泊研究所 | Construction method of cyanobacteria bloom prevention and control integrated technology system |
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