CN108671169A - A kind of preparation method of the ginseng peptide with effects of dispelling effects of alcohol and protecting liver - Google Patents

A kind of preparation method of the ginseng peptide with effects of dispelling effects of alcohol and protecting liver Download PDF

Info

Publication number
CN108671169A
CN108671169A CN201810646699.7A CN201810646699A CN108671169A CN 108671169 A CN108671169 A CN 108671169A CN 201810646699 A CN201810646699 A CN 201810646699A CN 108671169 A CN108671169 A CN 108671169A
Authority
CN
China
Prior art keywords
ginseng
effects
added
preparation
alcohol
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
CN201810646699.7A
Other languages
Chinese (zh)
Inventor
石丰
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CN201810646699.7A priority Critical patent/CN108671169A/en
Publication of CN108671169A publication Critical patent/CN108671169A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A61K31/365—Lactones
    • A61K31/375—Ascorbic acid, i.e. vitamin C; Salts thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/702—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56—Materials from animals other than mammals
    • A61K35/63—Arthropods
    • A61K35/64—Insects, e.g. bees, wasps or fleas
    • A61K35/644—Beeswax; Propolis; Royal jelly; Honey
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06—Fungi, e.g. yeasts
    • A61K36/07—Basidiomycota, e.g. Cryptococcus
    • A61K36/074—Ganoderma
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
    • A61K36/258—Panax (ginseng)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A61K36/288—Taraxacum (dandelion)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/488—Pueraria (kudzu)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/60—Moraceae (Mulberry family), e.g. breadfruit or fig
    • A61K36/605—Morus (mulberry)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A61K36/736—Prunus, e.g. plum, cherry, peach, apricot or almond
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/75—Rutaceae (Rue family)
    • A61K36/752—Citrus, e.g. lime, orange or lemon
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
    • A61K36/8998—Hordeum (barley)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • A61P39/02—Antidotes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/13—Preparation or pretreatment of starting material involving cleaning, e.g. washing or peeling
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying

Landscapes

  • Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Medical Informatics (AREA)
  • Biotechnology (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Insects & Arthropods (AREA)
  • Organic Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Animal Husbandry (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

The invention discloses a kind of preparation methods of the ginseng peptide with effects of dispelling effects of alcohol and protecting liver.The ginseng peptide preparation method includes：The preparation of ginseng pulverate；The preparation of the concentrated liquid of tradition Chinese medicine；The preparation of dried orange peel extracts；The preparation of enzymolysis and ginseng peptide.Ginseng peptide provided by the invention has reached good dispelling effects of alcohol by the collocation of ginseng peptide and Chinese medicine, has effects that protect well to alcohol-induced hepatic injury, in addition, adjustable body function, nutrition is high, and tonic effect is good；The selection of the combination of enzyme and dosage in enzymolysis so that enzymolysis efficiency and Degree of Enzymatic Hydrolysis are high, and in zymolyte amino chemicals type and quantity it is more, it is in good taste.The preparation method technique of ginseng peptide provided by the invention is advanced, process is reasonable, operability is strong, is easy to industrialized production.

Description

A kind of preparation method of the ginseng peptide with effects of dispelling effects of alcohol and protecting liver
Technical field
The present invention relates to ginseng products, more particularly, to a kind of preparation side of the ginseng peptide with effects of dispelling effects of alcohol and protecting liver Method.
Background technology
With the quickening of people's Working Life rhythm, operating pressure is big, and amount of exercise is reduced, and the constitution of people is begun to decline, Sub-health population increases sharply, and therefore, it is necessary to the high tonics of edible nutritional value to improve immunity of human body itself, build up health, carry High quality of life, and ginseng mends former QI invigorating, promoting production of body fluid and inducing sedation of the mind, vasodilator, excitor nerve, improvement blood circulation, increasing due to having The multiple efficacies such as strong heart contraction, usually act on the first choice of people.
Ginseng instructions of taking is generally oral, and it is low that the protein in food or drug, which will generally be digested liquid enzymolysis, Poly- peptide or amino acid and be absorbed by organisms and utilize, and above-mentioned indication crowd is common weakness of the spleen and the stomach or has gastrointestinal reaction, because Active low, the digestible energy force difference of this digestive enzyme, therefore oral ginseng not only results in the burden of people digest's system, and Also due drug effect is not achieved.
And ginseng is obtained small molecule ginseng peptide by processing can effectively overcome the above problem, come for mankind's health care belt Gospel.But the enzymolysis efficiency and Degree of Enzymatic Hydrolysis of existing ginseng peptide production method are low, the type of amino chemicals in zymolyte It is few with quantity, and poor taste improves nutrition and tonic effect simultaneously in addition, how to organically combine Chinese medicine and ginseng Protecting and nourishing liver simultaneously is the important technological problems of numerous technical staff's research.
Invention content
It is an object of the present invention to provide a kind of new technologies of the preparation method of the ginseng peptide with effects of dispelling effects of alcohol and protecting liver Scheme.
According to the first aspect of the invention, a kind of preparation method of the ginseng peptide with effects of dispelling effects of alcohol and protecting liver is provided, is wrapped Include following steps：
(1) it is cut into the sheet of 5cm thickness after drying ginseng wash clean, carries out ultramicro grinding, crosses 400 mesh sieve, obtains people Join powder；
(2) raw material of following weight proportion is weighed：3 parts of pueraria lobata, 4 parts of mulberry leaf, 4 parts of coloured malt, 3 parts of fingered citron, polyporus lucidus 2 3 parts of part, 3 parts of dark plum, 1.2 parts of dandelion, 0.3 part of vitamin C, 5 parts of honey and oligoisomaltose；By pueraria lobata, mulberry leaf, stir-fry Malt, fingered citron, polyporus lucidus, dark plum, dandelion are uniformly mixed, and extracting in water is positioned in extractor after cleaning twice, for the first time Add the water of 8 times of weight of composition total weight, second of the water for adding 6 times of weight of composition total weight, extraction 2 hours, make every time Liquid is obtained, liquid is filtered, merges the liquid decocted twice, relative density is 1.0 when being concentrated under reduced pressure into 60 DEG C, is concentrated under reduced pressure Condition is 60-70 DEG C, and 0.05-0.07MPa obtains Chinese medical extract；
(3) weigh the dried orange peel of 2 parts of parts by weight, coarse crushing adds the water of 5 times of amounts, extracting in boiling water, when extraction external condensing unit, Collect the condensate liquid containing volatile oil component, stop extraction after 1h, filter to take condensate liquid, concentrate filtrate, will dried orange peel extraction Object；
(4) it weighs ginseng pulverate made from step (1) according to formula ratio to be added in preparing tank, the pure water of formula ratio is added, adds Enter proteolytic enzyme enzymolysis 2h, then heats to 95 DEG C, enzyme deactivation 30min is let cool；Traditional Chinese medicine extraction will be obtained made from step (2) The honey of object and formula ratio, oligoisomaltose are added in preparing tank, stir to whole dissolvings, are warming up to 95 DEG C, 10-15min After sterilize, sodium bicarbonate is added after sterilizing and adjusts pH value to 6.0, the mixture of alpha-amylase and compound protease is added 2h is digested, the compound protease uses Protamex, then heats to 95 DEG C, enzyme deactivation 30min is let cool；Citric acid tune is added PH value is saved to 4.0, the mixture that pectase and restriction endonuclease is added digests 2h, then heats to 95 DEG C, enzyme deactivation 30min is let cool； The proteolytic enzyme, alpha-amylase, compound protease, pectase, restriction endonuclease and the mass ratio of ginseng pulverate are 11:3：5： 3：6：960；
(5) step (4) is let cool into liquid filtering, filtrate freeze-drying stirs evenly with dried orange peel extracts, vitamin C, tool is made Have, the ginseng peptide of effects of dispelling effects of alcohol and protecting liver.
Preferably, further include following processing step before ginseng carries out ultramicro grinding：
Sheet ginseng is sent into tumbler and carries out tumbling processing, when tumbling vacuumized primary every 10 minutes, exhausted every time Tumbling 15 minutes after vacuum, then vacuum pumping, so recycles 2-4 times.
Preferably, further include following processing step after ginseng carries out ultramicro grinding：
Ginseng pulverate is sent into microwave processing equipment, microwave treatment is spaced under microwave frequency 2500MHz, power 500W, Interval time is 8 minutes, and each microwave treatment 8 minutes is carried out continuously 3 times.
Ginseng peptide provided by the invention has reached good dispelling effects of alcohol by the collocation of ginseng peptide and Chinese medicine, to alcohol The hepatic injury of induction has effects that protect well, in addition, adjustable body function, nutrition is high, and tonic effect is good；In enzymolysis The combination of enzyme and the selection of dosage so that enzymolysis efficiency and Degree of Enzymatic Hydrolysis are high, and in zymolyte amino chemicals type sum number Amount is more, in good taste.The preparation method technique of ginseng peptide provided by the invention is advanced, process is reasonable, operability is strong, is easy to work Industry metaplasia is produced.
Specific implementation mode
Embodiment 1
A kind of ginseng peptide with effects of dispelling effects of alcohol and protecting liver, the composition is by following weight grams at being grouped as：
Ginseng Gly-His-Lys 24g, pueraria lobata 3g, mulberry leaf 4g, coloured malt 4g, fingered citron 3g, polyporus lucidus 2g, dark plum 3g, dandelion 1.2g, vitamin C 0.3g, dried orange peel 2g, honey 5g, oligoisomaltose 3g, pure water 55g.
The preparation method of the composition includes the following steps：
(1) sheet of 5cm thickness is cut into after drying ginseng wash clean, sheet ginseng, which is sent into tumbler, carries out tumbling Processing, when tumbling, vacuumized primary every 10 minutes, exhausted tumbling 15 minutes after vacuum every time, then vacuum pumping, so recycles 2-4 times；Ultramicro grinding is carried out, 400 mesh sieve is crossed, obtains ginseng pulverate；Ginseng pulverate is sent into microwave processing equipment, in Microwave Frequency Microwave treatment is spaced under rate 2500MHz, power 500W, interval time is 8 minutes, and each microwave treatment 8 minutes is carried out continuously 3 It is secondary, obtain the ginseng pulverate by microwave treatment；
(2) pueraria lobata, coloured malt, fingered citron, polyporus lucidus, dark plum and dandelion are weighed by pueraria lobata, mulberry leaf, stir-fry according to formula ratio Malt, fingered citron, polyporus lucidus, dark plum, dandelion are uniformly mixed, and extracting in water is positioned in extractor after cleaning twice, for the first time Add the water of 8 times of weight of composition total weight, second of the water for adding 6 times of weight of composition total weight, extraction 2 hours, are made every time Liquid filters liquid, merges the liquid decocted twice, and relative density is 1.0 when being concentrated under reduced pressure into 60 DEG C, and item is concentrated under reduced pressure Part is 65 DEG C, and 0.06MPa obtains Chinese medical extract；
(3) weigh dried orange peel according to formula ratio, coarse crushing adds the water of 5 times of amounts, extracting in boiling water, when extraction external condensing unit, Collect the condensate liquid containing volatile oil component, stop extraction after 1h, filter to take condensate liquid, concentrate filtrate, will dried orange peel extraction Object；
(4) ginseng pulverate that microwave treatment made from step (1) is weighed according to formula ratio is added in preparing tank, and formula ratio is added Pure water, be added proteolytic enzyme digest 2h, then heat to 95 DEG C, enzyme deactivation 30min is let cool；Step (2) are made Honey, the oligoisomaltose of Chinese medical extract and formula ratio is added in preparing tank, stir to whole dissolvings, be warming up to It 95 DEG C, sterilizes after 14min, sodium bicarbonate is added after sterilizing and adjusts pH value to 6.0, alpha-amylase and compound protein is added The mixture of enzyme digests 2h, and the compound protease uses Protamex, then heats to 95 DEG C, enzyme deactivation 30min is let cool； Lemon acid for adjusting pH value is added to 4.0, the mixture that pectase and restriction endonuclease is added digests 2h, then heats to 95 DEG C, enzyme deactivation 30min is let cool；The proteolytic enzyme, alpha-amylase, compound protease, pectase, restriction endonuclease and the quality of ginseng pulverate Than being 11:3：5：3：6：960；
(5) step (4) is let cool into liquid filtering, filtrate freeze-drying stirs evenly with dried orange peel extracts, vitamin C, tool is made Have, the ginseng peptide of effects of dispelling effects of alcohol and protecting liver.
It should be noted that the proportioning of ginseng Gly-His-Lys is it is known that and the formula ratio of ginseng pulverate is according to ginseng pulverate to ginseng peptide The calculation of yield of powder obtains, and specific yield by obtaining as follows：It weighs ginseng pulverate made from a certain amount of step (1) and preparation is added In tank, the pure water of formula ratio is added, proteolytic enzyme is added and digests 2h, then heats to 95 DEG C, enzyme deactivation 30min is put It is cold；Sodium bicarbonate is added and adjusts pH value to 6.0, the mixture that alpha-amylase and compound protease is added digests 2h, described compound Protease uses Protamex, then heats to 95 DEG C, enzyme deactivation 30min is let cool；Lemon acid for adjusting pH value is added to 4.0, adds The mixture enzymolysis 2h for entering pectase and restriction endonuclease, then heats to 95 DEG C, enzyme deactivation 30min lets cool, obtains enzymolysis liquid；It is described Proteolytic enzyme, alpha-amylase, compound protease, pectase, restriction endonuclease and the mass ratio of ginseng pulverate are 11:3：5：3：6： 960；Then enzymolysis liquid concentration drying obtains ginseng Gly-His-Lys, and the rate of output can be obtained according to the amount of ginseng Gly-His-Lys and ginseng pulverate.
Embodiment 2
A kind of ginseng peptide with effects of dispelling effects of alcohol and protecting liver, the composition is by following weight grams at being grouped as：
Ginseng Gly-His-Lys 20g, pueraria lobata 2g, mulberry leaf 2g, coloured malt 3g, fingered citron 2g, polyporus lucidus 1g, dark plum 2g, dandelion 1g, Vitamin C 0.1g, dried orange peel 2g, honey 4g, oligoisomaltose 2.5g, pure water 53g.
The preparation method of the composition includes the following steps：
(1) sheet of 5cm thickness is cut into after drying ginseng wash clean, sheet ginseng, which is sent into tumbler, carries out tumbling Processing, when tumbling, vacuumized primary every 10 minutes, exhausted tumbling 15 minutes after vacuum every time, then vacuum pumping, so recycles 2-4 times；Ultramicro grinding is carried out, 400 mesh sieve is crossed, obtains ginseng pulverate；Ginseng pulverate is sent into microwave processing equipment, in Microwave Frequency Microwave treatment is spaced under rate 2500MHz, power 500W, interval time is 8 minutes, and each microwave treatment 8 minutes is carried out continuously 3 It is secondary, obtain the ginseng pulverate by microwave treatment；
(2) pueraria lobata, coloured malt, fingered citron, polyporus lucidus, dark plum and dandelion are weighed by pueraria lobata, mulberry leaf, stir-fry according to formula ratio Malt, fingered citron, polyporus lucidus, dark plum, dandelion are uniformly mixed, and extracting in water is positioned in extractor after cleaning twice, for the first time Add the water of 8 times of weight of composition total weight, second of the water for adding 6 times of weight of composition total weight, extraction 2 hours, are made every time Liquid filters liquid, merges the liquid decocted twice, and relative density is 1.0 when being concentrated under reduced pressure into 60 DEG C, and item is concentrated under reduced pressure Part is 60 DEG C, and 0.05MPa obtains Chinese medical extract；
(3) weigh dried orange peel according to formula ratio, coarse crushing adds the water of 5 times of amounts, extracting in boiling water, when extraction external condensing unit, Collect the condensate liquid containing volatile oil component, stop extraction after 1h, filter to take condensate liquid, concentrate filtrate, will dried orange peel extraction Object；
(4) it weighs ginseng pulverate made from step (1) according to formula ratio to be added in preparing tank, the pure water of formula ratio is added, adds Enter proteolytic enzyme enzymolysis 2h, then heats to 95 DEG C, enzyme deactivation 30min is let cool；Traditional Chinese medicine extraction will be obtained made from step (2) The honey of object and formula ratio, oligoisomaltose are added in preparing tank, stir to whole dissolvings, are warming up to 95 DEG C, after 10min It sterilizes, sodium bicarbonate is added after sterilizing and adjusts pH value to 6.0, the mixture enzyme of alpha-amylase and compound protease is added 2h is solved, the compound protease uses Protamex, then heats to 95 DEG C, enzyme deactivation 30min is let cool；Citric acid is added to adjust For pH value to 4.0, the mixture that pectase and restriction endonuclease is added digests 2h, then heats to 95 DEG C, enzyme deactivation 30min is let cool；Institute It is 11 to state proteolytic enzyme, alpha-amylase, compound protease, pectase, restriction endonuclease and the mass ratio of ginseng pulverate:3：5：3： 6：960；
(5) step (4) is let cool into liquid filtering, filtrate freeze-drying stirs evenly with dried orange peel extracts, vitamin C, tool is made Have, the ginseng peptide of effects of dispelling effects of alcohol and protecting liver.
Embodiment 3
A kind of ginseng peptide with effects of dispelling effects of alcohol and protecting liver, the composition is by following weight grams at being grouped as：
Ginseng Gly-His-Lys 25g, pueraria lobata 5g, mulberry leaf 5g, coloured malt 4g, fingered citron 4g, polyporus lucidus 3g, dark plum 3g, dandelion 1.5g, vitamin C 0.5g, dried orange peel 4g, honey 6g, oligoisomaltose 3.5g, pure water 57g.
The preparation method of the composition includes the following steps：
(1) sheet of 5cm thickness is cut into after drying ginseng wash clean, sheet ginseng, which is sent into tumbler, carries out tumbling Processing, when tumbling, vacuumized primary every 10 minutes, exhausted tumbling 15 minutes after vacuum every time, then vacuum pumping, so recycles 2-4 times；Ultramicro grinding is carried out, 400 mesh sieve is crossed, obtains ginseng pulverate；Ginseng pulverate is sent into microwave processing equipment, in Microwave Frequency Microwave treatment is spaced under rate 2500MHz, power 500W, interval time is 8 minutes, and each microwave treatment 8 minutes is carried out continuously 3 It is secondary, obtain the ginseng pulverate by microwave treatment；
(2) pueraria lobata, coloured malt, fingered citron, polyporus lucidus, dark plum and dandelion are weighed by pueraria lobata, mulberry leaf, stir-fry according to formula ratio Malt, fingered citron, polyporus lucidus, dark plum, dandelion are uniformly mixed, and extracting in water is positioned in extractor after cleaning twice, for the first time Add the water of 8 times of weight of composition total weight, second of the water for adding 6 times of weight of composition total weight, extraction 2 hours, are made every time Liquid filters liquid, merges the liquid decocted twice, and relative density is 1.0 when being concentrated under reduced pressure into 60 DEG C, and item is concentrated under reduced pressure Part is 60-70 DEG C, and 0.05-0.07MPa obtains Chinese medical extract；
(3) weigh dried orange peel according to formula ratio, coarse crushing adds the water of 5 times of amounts, extracting in boiling water, when extraction external condensing unit, Collect the condensate liquid containing volatile oil component, stop extraction after 1h, filter to take condensate liquid, concentrate filtrate, will dried orange peel extraction Object；
(4) it weighs ginseng pulverate made from step (1) according to formula ratio to be added in preparing tank, the pure water of formula ratio is added, adds Enter proteolytic enzyme enzymolysis 2h, then heats to 95 DEG C, enzyme deactivation 30min is let cool；Traditional Chinese medicine extraction will be obtained made from step (2) The honey of object and formula ratio, oligoisomaltose are added in preparing tank, stir to whole dissolvings, are warming up to 95 DEG C, 10-15min After sterilize, sodium bicarbonate is added after sterilizing and adjusts pH value to 6.0, the mixture of alpha-amylase and compound protease is added 2h is digested, the compound protease uses Protamex, then heats to 95 DEG C, enzyme deactivation 30min is let cool；Citric acid tune is added PH value is saved to 4.0, the mixture that pectase and restriction endonuclease is added digests 2h, then heats to 95 DEG C, enzyme deactivation 30min is let cool； The proteolytic enzyme, alpha-amylase, compound protease, pectase, restriction endonuclease and the mass ratio of ginseng pulverate are 11:3：5： 3：6：960；
(5) step (4) is let cool into liquid filtering, filtrate freeze-drying stirs evenly with dried orange peel extracts, vitamin C, tool is made Have, the ginseng peptide of effects of dispelling effects of alcohol and protecting liver.
Reference examples 1
A kind of ginseng peptide with effects of dispelling effects of alcohol and protecting liver, the composition is by following weight grams at being grouped as：
Pueraria lobata 3g, mulberry leaf 4g, coloured malt 4g, fingered citron 3g, polyporus lucidus 2g, dark plum 3g, dandelion 1.2g, vitamin C0.3g, dried orange peel 2g, honey 5g, oligoisomaltose 3g, pure water 55g.
Reference examples 2
A kind of ginseng peptide with effects of dispelling effects of alcohol and protecting liver, the composition is by following weight grams at being grouped as：
Ginseng Gly-His-Lys 24g, pueraria lobata 3g, coloured malt 4g, fingered citron 3g, polyporus lucidus 2g, dark plum 3g, dandelion 1.2g, dried orange peel 2g, honey 5g, oligoisomaltose 3g, pure water 55g.
Reference examples 3
A kind of ginseng peptide with effects of dispelling effects of alcohol and protecting liver, the composition is by following weight grams at being grouped as：
Pueraria lobata 3g, mulberry leaf 4g, coloured malt 4g, polyporus lucidus 2g, dark plum 3g, dried orange peel 2g, honey 5g, oligoisomaltose 3g, Pure water 55g.
Reference examples 4
A kind of ginseng peptide with effects of dispelling effects of alcohol and protecting liver, the composition is by following weight grams at being grouped as：
Ginseng Gly-His-Lys 3g, pueraria lobata 1g, mulberry leaf 1g, coloured malt 1g, fingered citron 2g, polyporus lucidus 1g, dark plum 1g, dandelion 1g, dimension Raw element C0.1g, dried orange peel 2g, honey 1g, oligoisomaltose 2.5g, pure water 53g.
Reference examples 5
A kind of ginseng peptide with effects of dispelling effects of alcohol and protecting liver, the composition is by following weight grams at being grouped as：
Ginseng Gly-His-Lys 35g, pueraria lobata 5g, mulberry leaf 25g, coloured malt 8g, fingered citron 14g, polyporus lucidus 3g, dark plum 8g, dandelion 15g, vitamin C 5g, dried orange peel 4g, honey 6g, oligoisomaltose 3.5g, pure water 60g.
Reference examples 6
A kind of ginseng peptide with effects of dispelling effects of alcohol and protecting liver, the composition is by following weight grams at being grouped as：
Ginseng Gly-His-Lys 24g, pueraria lobata 3g, Cortex Eucommiae 4g, coloured malt 4g, fingered citron 3g, polyporus lucidus 2g, dark plum 3g, dandelion 1.2g, vitamin C 0.3g, dried orange peel 2g, honey 5g, oligoisomaltose 3g, pure water 55g, cassia twig 4g.
Reference examples 7
A kind of ginseng peptide with effects of dispelling effects of alcohol and protecting liver, the composition is by following weight grams at being grouped as：
Ginseng Gly-His-Lys 24g, pueraria lobata 3g, mulberry leaf 4g, coloured malt 4g, fingered citron 3g, polyporus lucidus 2g, dark plum 3g, dandelion 1.2g, vitamin C 0.3g, dried orange peel 2g, honey 5g, oligoisomaltose 3g, pure water 55g, Hedyotidis Diffusae 4g.
The preparation method of ginseng peptides of the reference examples 1-7 with effects of dispelling effects of alcohol and protecting liver is on the basis of embodiment 1 according to component Variation accordingly changed.
Reference examples 8
A kind of ginseng peptide with effects of dispelling effects of alcohol and protecting liver, the composition is by following weight grams at being grouped as：
Ginseng Gly-His-Lys 24g, pueraria lobata 3g, mulberry leaf 4g, coloured malt 4g, fingered citron 3g, polyporus lucidus 2g, dark plum 3g, dandelion 1.2g, vitamin C 0.3g, honey 5g, oligoisomaltose 3g, pure water 55g.
The preparation method of the composition includes the following steps：
(1) sheet of 5cm thickness is cut into after drying ginseng wash clean, sheet ginseng, which is sent into tumbler, carries out tumbling Processing, when tumbling, vacuumized primary every 10 minutes, exhausted tumbling 15 minutes after vacuum every time, then vacuum pumping, so recycles 2-4 times；Ultramicro grinding is carried out, 400 mesh sieve is crossed, obtains ginseng pulverate；Ginseng pulverate is sent into microwave processing equipment, in Microwave Frequency Microwave treatment is spaced under rate 2500MHz, power 500W, interval time is 8 minutes, and each microwave treatment 8 minutes is carried out continuously 3 It is secondary, obtain the ginseng pulverate by microwave treatment；
2) pueraria lobata, coloured malt, fingered citron, polyporus lucidus, dark plum and dandelion are weighed by pueraria lobata, mulberry leaf, stir-fry wheat according to formula ratio Bud, fingered citron, polyporus lucidus, dark plum, dandelion are uniformly mixed, and extracting in water in extractor is positioned over after cleaning and twice, is added for the first time The water of 8 times of weight of composition total weight, second of the water for adding 6 times of weight of composition total weight, extraction 2 hours, are made medicine every time Liquid filters liquid, merges the liquid decocted twice, and relative density is 1.0 when being concentrated under reduced pressure into 60 DEG C, and condition is concentrated under reduced pressure It it is 65 DEG C, 0.06MPa obtains Chinese medical extract；
(3) it weighs ginseng pulverate made from step (1) according to formula ratio to be added in preparing tank, the pure water of formula ratio is added, adds Enter proteolytic enzyme enzymolysis 2h, then heats to 95 DEG C, enzyme deactivation 30min is let cool；Traditional Chinese medicine extraction will be obtained made from step (2) The honey of object and formula ratio, oligoisomaltose are added in preparing tank, stir to whole dissolvings, are warming up to 95 DEG C, after 14min It sterilizes, sodium bicarbonate is added after sterilizing and adjusts pH value to 6.0, the mixture enzyme of alpha-amylase and compound protease is added 2h is solved, the compound protease uses Protamex, then heats to 95 DEG C, enzyme deactivation 30min is let cool；Citric acid is added to adjust For pH value to 4.0, the mixture that pectase and restriction endonuclease is added digests 2h, then heats to 95 DEG C, enzyme deactivation 30min is let cool；Institute It is 11 to state proteolytic enzyme, alpha-amylase, compound protease, pectase, restriction endonuclease and the mass ratio of ginseng pulverate:3：5：3： 6：960；
(4) step (3) is let cool into liquid filtering, the ginseng peptide with effects of dispelling effects of alcohol and protecting liver is made in filtrate freeze-drying.
Reference examples 9
A kind of ginseng peptide with effects of dispelling effects of alcohol and protecting liver, the composition is by following weight grams at being grouped as：
Ginseng Gly-His-Lys 20g, honey 4g, pure water 53g.
The preparation method of the composition includes the following steps：
(1) sheet of 5cm thickness is cut into after drying ginseng wash clean, sheet ginseng, which is sent into tumbler, carries out tumbling Processing, when tumbling, vacuumized primary every 10 minutes, exhausted tumbling 15 minutes after vacuum every time, then vacuum pumping, so recycles 2-4 times；Ultramicro grinding is carried out, 400 mesh sieve is crossed, obtains ginseng pulverate；Ginseng pulverate is sent into microwave processing equipment, in Microwave Frequency Microwave treatment is spaced under rate 2500MHz, power 500W, interval time is 8 minutes, and each microwave treatment 8 minutes is carried out continuously 3 It is secondary, obtain the ginseng pulverate by microwave treatment；
(2) it weighs ginseng pulverate made from step (1) according to formula ratio to be added in preparing tank, the pure water of formula ratio is added, adds Enter proteolytic enzyme enzymolysis 2h, then heats to 95 DEG C, enzyme deactivation 30min is let cool；Be added sodium bicarbonate adjust pH value to 6.0, the mixture that alpha-amylase and compound protease is added digests 2h, and the compound protease uses Protamex, then rises To 95 DEG C, enzyme deactivation 30min is let cool temperature；Lemon acid for adjusting pH value is added to 4.0, the mixture enzyme of pectase and restriction endonuclease is added 2h is solved, then heats to 95 DEG C, enzyme deactivation 30min is let cool；The proteolytic enzyme, alpha-amylase, compound protease, fruit Glue enzyme, restriction endonuclease and the mass ratio of ginseng pulverate are 11:3：5：3：6：960；
(3) step (2) is let cool into liquid filtering, the ginseng peptide with effects of dispelling effects of alcohol and protecting liver is made in filtrate freeze-drying.
Reference examples 10
A kind of ginseng peptide with effects of dispelling effects of alcohol and protecting liver, the composition is by following weight grams at being grouped as：
Ginseng Gly-His-Lys 25g, pueraria lobata 5g, mulberry leaf 5g, coloured malt 4g, fingered citron 4g, polyporus lucidus 3g, dark plum 3g, dandelion 1.5g, vitamin C 0.5g, dried orange peel 4g, honey 6g, oligoisomaltose 3.5g, pure water 57g.
The preparation method of the composition includes the following steps：
(1) sheet of 5cm thickness is cut into after drying ginseng wash clean, sheet ginseng, which is sent into tumbler, carries out tumbling Processing, when tumbling, vacuumized primary every 10 minutes, exhausted tumbling 15 minutes after vacuum every time, then vacuum pumping, so recycles 2-4 times；Ultramicro grinding is carried out, 400 mesh sieve is crossed, obtains ginseng pulverate；Ginseng pulverate is sent into microwave processing equipment, in Microwave Frequency Microwave treatment is spaced under rate 2500MHz, power 500W, interval time is 8 minutes, and each microwave treatment 8 minutes is carried out continuously 3 It is secondary, obtain the ginseng pulverate by microwave treatment；
(2) pueraria lobata, coloured malt, fingered citron, polyporus lucidus, dark plum and dandelion are weighed by pueraria lobata, mulberry leaf, stir-fry according to formula ratio Malt, fingered citron, polyporus lucidus, dark plum, dandelion are uniformly mixed, and extracting in water is positioned in extractor after cleaning twice, for the first time Add the water of 8 times of weight of composition total weight, second of the water for adding 6 times of weight of composition total weight, extraction 2 hours, are made every time Liquid filters liquid, merges the liquid decocted twice, and relative density is 1.0 when being concentrated under reduced pressure into 60 DEG C, and item is concentrated under reduced pressure Part is 60-70 DEG C, and 0.05-0.07MPa obtains Chinese medical extract；
(3) weigh dried orange peel according to formula ratio, coarse crushing adds the water of 5 times of amounts, extracting in boiling water, when extraction external condensing unit, Collect the condensate liquid containing volatile oil component, stop extraction after 1h, filter to take condensate liquid, concentrate filtrate, will dried orange peel extraction Object；
(4) it weighs ginseng pulverate made from step (1) according to formula ratio to be added in preparing tank, the pure water of formula ratio is added, adds Enter proteolytic enzyme enzymolysis 2h, then heats to 95 DEG C, enzyme deactivation 30min is let cool；Traditional Chinese medicine extraction will be obtained made from step (2) The honey of object and formula ratio, oligoisomaltose are added in preparing tank, stir to whole dissolvings, are warming up to 95 DEG C, 10-15min After sterilize, lemon acid for adjusting pH value is added after sterilizing to 4.0, the mixture that pectase and restriction endonuclease is added digests 2h, 95 DEG C are then heated to, enzyme deactivation 30min is let cool；The proteolytic enzyme, pectase, restriction endonuclease and the mass ratio of ginseng pulverate It is 11:3：5：3：6：960；
(5) step (4) is let cool into liquid filtering, filtrate freeze-drying stirs evenly with dried orange peel extracts, vitamin C, tool is made Have, the ginseng peptide of effects of dispelling effects of alcohol and protecting liver.
Reference examples 11
A kind of ginseng peptide with effects of dispelling effects of alcohol and protecting liver, the composition is by following weight grams at being grouped as：
Ginseng Gly-His-Lys 25g, pueraria lobata 5g, mulberry leaf 5g, coloured malt 4g, fingered citron 4g, polyporus lucidus 3g, dark plum 3g, dandelion 1.5g, vitamin C 0.5g, dried orange peel 4g, honey 6g, oligoisomaltose 3.5g, pure water 57g.
The preparation method of the composition includes the following steps：
(1) sheet of 5cm thickness is cut into after drying ginseng wash clean, sheet ginseng, which is sent into tumbler, carries out tumbling Processing, when tumbling, vacuumized primary every 10 minutes, exhausted tumbling 15 minutes after vacuum every time, then vacuum pumping, so recycles 2-4 times；Ultramicro grinding is carried out, 400 mesh sieve is crossed, obtains ginseng pulverate；Ginseng pulverate is sent into microwave processing equipment, in Microwave Frequency Microwave treatment is spaced under rate 2500MHz, power 500W, interval time is 8 minutes, and each microwave treatment 8 minutes is carried out continuously 3 It is secondary, obtain the ginseng pulverate by microwave treatment；
(2) pueraria lobata, coloured malt, fingered citron, polyporus lucidus, dark plum and dandelion are weighed by pueraria lobata, mulberry leaf, stir-fry according to formula ratio Malt, fingered citron, polyporus lucidus, dark plum, dandelion are uniformly mixed, and extracting in water is positioned in extractor after cleaning twice, for the first time Add the water of 8 times of weight of composition total weight, second of the water for adding 6 times of weight of composition total weight, extraction 2 hours, are made every time Liquid filters liquid, merges the liquid decocted twice, and relative density is 1.0 when being concentrated under reduced pressure into 60 DEG C, and item is concentrated under reduced pressure Part is 60-70 DEG C, and 0.05-0.07MPa obtains Chinese medical extract；
(3) weigh dried orange peel according to formula ratio, coarse crushing adds the water of 5 times of amounts, extracting in boiling water, when extraction external condensing unit, Collect the condensate liquid containing volatile oil component, stop extraction after 1h, filter to take condensate liquid, concentrate filtrate, will dried orange peel extraction Object；
(4) it weighs ginseng pulverate made from step (1) according to formula ratio to be added in preparing tank, the pure water of formula ratio is added, adds Enter proteolytic enzyme enzymolysis 2h, then heats to 95 DEG C, enzyme deactivation 30min is let cool；Traditional Chinese medicine extraction will be obtained made from step (2) The honey of object and formula ratio, oligoisomaltose are added in preparing tank, stir to whole dissolvings, are warming up to 95 DEG C, 10-15min After sterilize, lemon acid for adjusting pH value is added after sterilizing to 4.0, the mixture that pectase and restriction endonuclease is added digests 2h, 95 DEG C are then heated to, enzyme deactivation 30min is let cool；Sodium bicarbonate is added and adjusts pH value to 6.0, alpha-amylase and compound egg is added The mixture of white enzyme digests 2h, and the compound protease uses Protamex, then heats to 95 DEG C, enzyme deactivation 30min is let cool； The proteolytic enzyme, α-amylase, compound protease, pectase, restriction endonuclease and the mass ratio of ginseng pulverate are 3：11:3： 5： 6：960；
(5) step (4) is let cool into liquid filtering, filtrate freeze-drying stirs evenly with dried orange peel extracts, vitamin C, tool is made Have, the ginseng peptide of effects of dispelling effects of alcohol and protecting liver.
Test example 1：Alcohol concentration reduces experiment
Test method：The ginseng peptide of 1-3 of the embodiment of the present invention and reference examples 1-7 is taken to be respectively added to that white wine (50 °) is housed In 50ml vessel, measure the concentration that ethyl alcohol in front and back white wine is added, time point of measurement is respectively 5 minutes, 15 minutes, it is one small When, one day.The height of final ethanol content represents the height of the ability of relieving the effect of alcohol of experiment health products, referring to the following table 1.
1 alcohol concentration of table reduces experiment
As shown in Table 1, the effective of reduction alcohol of the ginseng peptide of 1-3 of the embodiment of the present invention is higher than reference examples 1-7 Composition, it was demonstrated that ginseng peptide provided by the invention have good Dealcoholic sobering-up effect, when in ginseng peptide provided by the invention Component increase, reduce or be replaced by other group of timesharing, the effective reduction of the composition Dealcoholic sobering-up；Embodiment 1 Effect is better than experimental example 2 and experimental example 3, it was demonstrated that the ginseng peptide Dealcoholic sobering-up effect of embodiment 1 is best.
Test example 2：Zoopery to the protective effect of acute liver damage
1. 50% alcohol of materials and methods is spare；The ginseng peptide of 1-3 of the embodiment of the present invention and comparative example 1-11.Used time will It prepares 10 multiple doses of adult's recommended dose, is 360mg/kg.Choose cleaning grade ICR mouse 15, male, weight 18- 22g is randomly divided into 3 groups：A groups (blank control group) 1, B groups (test group) 3, C groups (control group) 11.Each group mouse with Fed standard chow, free water, adaptable fed enter experiment after 1 week.Each group mouse fasting 8h, A group is given before experiment Distilled water, B groups mouse are given the ginseng peptide of 1-6 of the embodiment of the present invention by setting dosage gavage respectively, and C groups mouse is respectively by setting Determine the ginseng peptide that dosage gavage gives comparative example 1-11 of the present invention.After gavage 45min, in addition to A group mouse give distilled water, Its each group mouse gives 50% ethanol solution by the dosage of 10ml/kg.
Mouse liver is won after measuring ethyl alcohol gavage 12h, 10% liver homogenate is made, measures malonaldehyde in hepatic tissue (MDA), reduced glutathione (GSH) and serum triglyceride (TG) content, the wherein measurement of serum triglyceride are using sweet Oleophosphoric acid oxidizing ferment peroxidase method, MDA, which is measured, uses TBA colorimetric methods, and GSH, which is measured, uses DTNB colorimetric methods.
Influence to animal lipid and antioxidant system, animal's liver malonaldehyde, reduced glutathione and serum glycerine Difference is statistically significant (P ＜ 0.05) compared with the control group for three ester contents, the results are shown in Table 2.
The influence of 2 murine liver tissue GSH, MDA of table and serum TG
Compared with blank control group, the ginseng peptide of test group can significantly reduce malonaldehyde (MDA) and serum triglyceride (TG) content improves reduced glutathione (GSH) content, the ginseng peptide of test group is prompted to have alcohol-induced hepatic injury Apparent protecting effect；Compared with the control group, the ginseng peptide of test group there is preferably protection to imitate alcohol-induced hepatic injury Fruit, when in ginseng peptide provided by the invention component increase, reduce or be replaced by other group of timesharing, the composition is to alcohol The effective reduction of liver injury protection of induction, in addition, the preparation method of the present invention can improve the enzymolysis efficiency of ginseng peptide And Degree of Enzymatic Hydrolysis, it can be improved liver injury protection effect, can not arbitrarily change with component the step of preparation method.
Test example 3：Human feeding trial
Test method：Enter 50 test-meal observations of group.Age 18-55 Sui, wherein man 40, Ms 10.
Enter a group condition：It drinks history with 3-30, frequent alcohol user, except having hepatopathy, hyperpietic, nothing during test-meal Fatigue or other uncomfortable situations, the test-meal present invention is preceding to be stopped drinking any wine 3 days.
The test-meal time：It drinks test-meal after selection supper or supper.
Test-meal experiment after drunk：Respectively drunk mild degree, in, weight when the test-meal present invention the people with relieving alcoholism and protecting liver Join peptide.It is dizzy, excited, belong to slight degree；Flushed, tongue hair is straight, and more than category of speaking is slight；Babble, the dimness of vision, Obnubilation belongs to moderate；It vomits, is dead drunk, senseless for severe.
Instructions of taking：It is sent with 300-400 milliliters of warm water clothes.
The improvement situation of drunk symptom after 3 test-meal of table ginseng peptide of the present invention：
Table 3
Conclusion：The ginseng peptide of the present invention can preferably improve drunk symptom, efficient average out to 96%.

Claims (3)

1. a kind of preparation method of the ginseng peptide with effects of dispelling effects of alcohol and protecting liver, which is characterized in that include the following steps：
(1) it is cut into the sheet of 5cm thickness after drying ginseng wash clean, carries out ultramicro grinding, crosses 400 mesh sieve, obtains ginseng pulverate；
(2) raw material of following weight proportion is weighed：3 parts of pueraria lobata, 4 parts of mulberry leaf, 4 parts of coloured malt, 3 parts of fingered citron, 2 parts of polyporus lucidus, crow 3 parts of 3 parts of plum, 1.2 parts of dandelion, 0.3 part of vitamin C, 5 parts of honey and oligoisomaltose；By pueraria lobata, mulberry leaf, coloured malt, Buddhist Hand, polyporus lucidus, dark plum, dandelion are uniformly mixed, and extracting in water in extractor is positioned over after cleaning and twice, adds composition for the first time The water of 8 times of weight of total weight, second of the water for adding 6 times of weight of composition total weight, extraction 2 hours, are made liquid, by medicine every time Liquid filters, and merges the liquid decocted twice, and relative density is 1.0 when being concentrated under reduced pressure into 60 DEG C, and reduced pressure condition is 60-70 DEG C, 0.05-0.07MPa obtains Chinese medical extract；
(3) weigh the dried orange peel of 2 parts of parts by weight, coarse crushing adds the water of 5 times of amounts, extracting in boiling water, when extraction external condensing unit, collect Condensate liquid containing volatile oil component stops extraction after 1h, filters to take condensate liquid, concentrates filtrate, will obtain dried orange peel extracts；
(4) it weighs ginseng pulverate made from step (1) according to formula ratio to be added in preparing tank, the pure water of formula ratio is added, egg is added White matter enzymatic hydrolysis 2h, then heats to 95 DEG C, and enzyme deactivation 30min is let cool；Will step (2) it is obtained Chinese medical extract with Honey, the oligoisomaltose of formula ratio are added in preparing tank, stir to whole dissolvings, are warming up to 95 DEG C, 10-15min is laggard Row sterilizing is added sodium bicarbonate and adjusts pH value to 6.0, the mixture enzymolysis of alpha-amylase and compound protease is added after sterilizing 2h, the compound protease use Protamex, then heat to 95 DEG C, enzyme deactivation 30min is let cool；Lemon acid for adjusting pH is added For value to 4.0, the mixture that pectase and restriction endonuclease is added digests 2h, then heats to 95 DEG C, enzyme deactivation 30min is let cool；The egg White matter hydrolase, alpha-amylase, compound protease, pectase, restriction endonuclease and the mass ratio of ginseng pulverate are 11:3：5：3：6：960；
(5) step (4) is let cool into liquid filtering, filtrate freeze-drying is stirred evenly with dried orange peel extracts, vitamin C, and being made has, solves The ginseng peptide of wine liver protection effect.
2. the preparation method of the ginseng peptide according to claim 1 with effects of dispelling effects of alcohol and protecting liver, which is characterized in that in ginseng Further include following processing step before carrying out ultramicro grinding：
Sheet ginseng is sent into tumbler and carries out tumbling processing, when tumbling vacuumized primary every 10 minutes, exhausted vacuum every time Tumbling 15 minutes afterwards, then vacuum pumping, so recycles 2-4 times.
3. the preparation method of the ginseng peptide according to claim 1 with effects of dispelling effects of alcohol and protecting liver, which is characterized in that in ginseng Further include following processing step after carrying out ultramicro grinding：
Ginseng pulverate is sent into microwave processing equipment, microwave treatment is spaced under microwave frequency 2500MHz, power 500W, is spaced Time is 8 minutes, and each microwave treatment 8 minutes is carried out continuously 3 times.
CN201810646699.7A 2018-06-21 2018-06-21 A kind of preparation method of the ginseng peptide with effects of dispelling effects of alcohol and protecting liver Withdrawn CN108671169A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810646699.7A CN108671169A (en) 2018-06-21 2018-06-21 A kind of preparation method of the ginseng peptide with effects of dispelling effects of alcohol and protecting liver

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201810646699.7A CN108671169A (en) 2018-06-21 2018-06-21 A kind of preparation method of the ginseng peptide with effects of dispelling effects of alcohol and protecting liver

Publications (1)

Publication Number Publication Date
CN108671169A true CN108671169A (en) 2018-10-19

Family

ID=63811977

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201810646699.7A Withdrawn CN108671169A (en) 2018-06-21 2018-06-21 A kind of preparation method of the ginseng peptide with effects of dispelling effects of alcohol and protecting liver

Country Status (1)

Country Link
CN (1) CN108671169A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110839896A (en) * 2019-12-26 2020-02-28 吉林大学 Preparation method of ginseng instant food for improving type II diabetes symptoms
JP2021073987A (en) * 2019-11-12 2021-05-20 禅インターナショナル株式会社 Nutritional composition
CN113142589A (en) * 2021-02-25 2021-07-23 广东维尔嘉康生命科技有限公司 Liver-protecting hexapeptide composition and preparation method thereof
CN115553461A (en) * 2021-02-04 2023-01-03 张晓宇 Preparation method of American ginseng peptide

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2021073987A (en) * 2019-11-12 2021-05-20 禅インターナショナル株式会社 Nutritional composition
CN110839896A (en) * 2019-12-26 2020-02-28 吉林大学 Preparation method of ginseng instant food for improving type II diabetes symptoms
CN115553461A (en) * 2021-02-04 2023-01-03 张晓宇 Preparation method of American ginseng peptide
CN113142589A (en) * 2021-02-25 2021-07-23 广东维尔嘉康生命科技有限公司 Liver-protecting hexapeptide composition and preparation method thereof

Similar Documents

Publication Publication Date Title
CN108671169A (en) A kind of preparation method of the ginseng peptide with effects of dispelling effects of alcohol and protecting liver
CN103540493B (en) A kind of compound method of ganoderma lucidum tea wine
CN102100805B (en) Composition for relieving physical fatigue and enhancing immunologic function, preparation method thereof and application thereof
CN103653147A (en) Wheat peptide anti-fatigue health drink and preparation method thereof
CN101416736A (en) Nutrient tonic candied date can and preparation method thereof
CN102228180A (en) Betelnut buccal tablet and preparation method thereof
CN104872758A (en) Preparation method of cordyceps sobolifera and burdock oral liquid
CN104000143A (en) Nourishing and cooling dry lotus root vermicelli and preparation method thereof
CN109169939A (en) A kind of preparation method of soybean milk powder
CN106343300A (en) Plant beverage with flos rosae rugosae and method for preparing plant beverage
CN1498544A (en) Biological engineering prepared health care milk as well as matched method and product
CN106260453A (en) Targeting repairs the Rhizoma Polygonati Fructus Rubi pressed candy of kidney
CN101503727B (en) Hyriopsis cumingii enzymolysis polypeptide, as well as preparation and application thereof
CN106473995A (en) The extracting method of Herba Bidentis Bipinnatae effective ingredient and its purposes preparing facial film
CN101513445A (en) Sea horse health maintaining liquid and preparation method thereof
CN103798520B (en) A kind of feed additive of substitute antibiotics and preparation method thereof
CN101756095A (en) Cactus noodle and production method thereof
CN107373259A (en) One kind relaxes bowel Chinese herbal health-caring beverage and preparation method thereof
CN113383956B (en) Kidney-tonifying formula of black truffle and preparation method thereof
CN108159301A (en) A kind of oral administration liquor for reducing blood fat and preparation method thereof
CN106983830A (en) Antidepression health products containing Rice Bran Flavonoid
CN107960623A (en) A kind of processing method of water chestnut cake
KR20130060811A (en) The method manufacture and an annex drink use pycnogenol have immunity and thrombus melting
CN102047990A (en) Traditional Chinese medicine health care tea for preventing and treating hyperlipidaemia
CN106615411A (en) Origin preserving and liver nourishing tea and method for preparing same

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
WW01 Invention patent application withdrawn after publication
WW01 Invention patent application withdrawn after publication

Application publication date: 20181019