CN108663305A - A kind of flow cytometer collector - Google Patents
A kind of flow cytometer collector Download PDFInfo
- Publication number
- CN108663305A CN108663305A CN201810824986.2A CN201810824986A CN108663305A CN 108663305 A CN108663305 A CN 108663305A CN 201810824986 A CN201810824986 A CN 201810824986A CN 108663305 A CN108663305 A CN 108663305A
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- China
- Prior art keywords
- compartment
- sorting
- flow cytometer
- collection cups
- collector according
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- 239000007788 liquid Substances 0.000 claims abstract description 35
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 230000010354 integration Effects 0.000 claims abstract description 7
- 230000007935 neutral effect Effects 0.000 claims description 11
- 238000012360 testing method Methods 0.000 description 18
- 239000002699 waste material Substances 0.000 description 8
- 239000012530 fluid Substances 0.000 description 3
- 238000005253 cladding Methods 0.000 description 2
- 239000000428 dust Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000003032 molecular docking Methods 0.000 description 2
- 230000010355 oscillation Effects 0.000 description 2
- 240000002853 Nelumbo nucifera Species 0.000 description 1
- 235000006508 Nelumbo nucifera Nutrition 0.000 description 1
- 235000006510 Nelumbo pentapetala Nutrition 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000010339 medical test Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1404—Handling flow, e.g. hydrodynamic focusing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1404—Handling flow, e.g. hydrodynamic focusing
- G01N2015/1415—Control of particle position
Landscapes
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of flow cytometer collectors, include the sorting bin of at least three compartments, its each compartment bottom of the sorting bin has been made into integration drain bucket;Two outer avris of the sorting bin is fixed with grip block;The grip block bottom is fixed to liquid collecting frame;The liquid collecting frame lower inside is fixed with flanged plate;It is screwed with screw rod on the flanged plate;The screw tip is fixed with stress cup;It is placed with collection cups on the inside of the stress cup;Face drain bucket at the top of the collection cups.The flow cytometer collector of the present invention can all be received deflecting plates dropping liquid, can't be polluted to ambient enviroment using the rectangular compartment of sorting bin, and the dropping liquid after collection cups are collected can carry out analysis experiment by the export of water conservancy diversion bucket.
Description
Technical field
The present invention relates to a kind of flow cytometer components, and in particular to it is thin to belong to streaming for a kind of flow cytometer collector
Born of the same parents' instrument component technology field.
Background technology
Flow cytometer forms charged drop containing cell to realize to the sorting of cell by a fluid stream;In tool point
It selects and is equipped on the nozzle of the flow cytometer flow chamber of function there are one sonic oscillation pressure crystal wafer, this oscillation device can incite somebody to action oneself
The liquid beam that nozzle projects is broken into ten million droplet, and the cell of flowing is also just dispersed in these small water droplets simultaneously.At this moment it gives
As soon as a fluid stream electric impulse signal makes small water droplet all take charge.When the cell liquid with different charges flow through a pair of of band just,
When bearing the deflecting plates of several kilovolts of constant static electric fields, the small water droplet of electrification just generates deflection according to the charge property of itself institute's band,
It falls into respective collection vessel, uncharged water droplet is put into intermediate waste fluid container, to realize point of cell
Choosing;The existing collector for flow cytometer is composed of test tube and flask, is caught respectively to different directions and is detached
Drop, since the electrically charged amount of drop institute is different, there is also difference for offset, and existing collector is collecting different charges
When the drop of amount, due to there is no the suitable collector of profession, the phenomenon that there are drop wastes, and be easy to be displaced to collection
External container pollutes workbench;For this purpose, Chinese Patent Application No.:201720073588.2 disclosing a kind of medicine inspection
Test the collector with flow cytometer, including waste beaker, level-one test tube, two level test tube, test tube slant and beaker inclined-plane, institute
State fixed plate and be equipped with the through-hole that matches with waste beaker, level-one test tube, two level test tube, and waste beaker, level-one test tube and
The top of two level test tube is fixed in through-hole, the waste beaker, level-one test tube and two level test tube top, be located at fixed plate on
And, the outside of the waste beaker is equipped with beaker location ring equipped with round table-like inclined-plane, the level-one test tube and two level test tube
Outside is equipped with test tube locating ring, passes through between test tube locating ring, between beaker location ring and test tube locating ring at described adjacent two
Connecting rod is connected.The collector of the medical test flow cytometer can be realized using level-one test tube and two level test tube to weak
The drop of charge and categorised collection with forceful electric power lotus drop, facilitate the later stage to carry out different experiments, further, it is possible to effectively reduce liquid
The waste of drop;But it is merely capable of completing drop categorised collection, but it still has and pollutes situation to collector outside.
Invention content
To solve the above problems, the present invention proposes a kind of flow cytometer collector, using the rectangular compartment of sorting bin,
It can all receive deflecting plates dropping liquid, can't be polluted to ambient enviroment, and the dropping liquid after collection cups are collected can be by leading
Stream bucket export carries out analysis experiment.
The flow cytometer collector of the present invention, includes the sorting bin of at least three compartments, its each compartment of the sorting bin
Bottom has been made into integration drain bucket;Its both sides compartment of sorting bin can be matched with deflecting plates, ensure accurate reception dropping liquid;Described point
Two outer avris of storehouse is selected to be fixed with grip block;The grip block bottom is fixed to liquid collecting frame;The liquid collecting frame lower inside is fixed with
Flanged plate;It is screwed with screw rod on the flanged plate;The screw tip is fixed with stress cup;It is placed with receipts on the inside of the stress cup
Collect cup;Face drain bucket at the top of the collection cups, is screwed on by screw rod on flanged plate, can stress cup be carried out rising-falling tone
It is whole, to ensure that collection cups are accurately docked with drain bucket.
As preferred embodiment, described its outer diameter of drain bucket is less than collection cups internal diameter, can fast implement docking.
As preferred embodiment, the sorting bin thirdly compartment be anode sorting compartment, cathode sort compartment and in
Property sorting compartment.
As preferred embodiment, the anode sorting compartment and cathode sorting compartment are far from neutral sorting compartment side
It has been made into integration sorting and has received and expanded outer;The sorting receives expansion outer and is set to immediately below deflecting plates;Sorting, which receives, expands
The entire bottom surface of deflecting plates can be coated by filling outer, ensure that collection liquid is more comprehensive.
As preferred embodiment, the anode sorting compartment and cathode sorting compartment top surface are less than neutral sorting compartment
Apical side height, anode sorting compartment and cathode sort compartment using cladding deflecting plates comprehensively, and by improving neutrality sorting compartment
Highly, neutral solution can be avoided to enter avris compartment.
As preferred embodiment, the collection cups are undergauge cup;Collection cups lower section at undergauge offers out
Liquid mouth;The collection cups are provided with water conservancy diversion bucket in liquid outlet lower outside, can avoid enter into dust, and leading by avris
Stream bucket can export collection liquid.
As preferred embodiment, the liquid collecting frame inboard width is more than the width between grip block.
As preferred embodiment, mounting slot is offered on the grip block, can be coordinated with flow cytometer and be pacified
Dress.
As preferred embodiment, the screw rod periphery is provided with elastic component;It is adjacent to flanged plate at the top of the elastic component
Bottom surface, the elastic component bottom are adjacent to screw rod its screw head.
As preferred embodiment, the stress cup, collection cups and drain bucket central shaft are in same straight line.
The present invention compared with prior art, flow cytometer collector of the invention, using the rectangular compartment of sorting bin,
It can all receive deflecting plates dropping liquid, and carry out receiving drop and guiding by sorting reception expansion outer, can complete just
The categorised collection of pole dropping liquid, cathode dropping liquid and neutral dropping liquid, the dropping liquid after collection cups are collected can be divided by the export of water conservancy diversion bucket
Analysis experiment.
Description of the drawings
Fig. 1 is the overall structure diagram of the present invention.
Specific implementation mode
Flow cytometer collector as shown in Figure 1, includes the sorting bin 1 of at least three compartments, its is each for the sorting bin 1
Compartment bottom has been made into integration drain bucket 2;Its both sides compartment of sorting bin 1 can be matched with deflecting plates, ensure accurate reception dropping liquid;
1 liang of outer avris of the sorting bin is fixed with grip block 3;3 bottom of the grip block is fixed to liquid collecting frame 4;4 inside of the liquid collecting frame
Lower part is fixed with flanged plate 5;Screw rod 6 is screwed on the flanged plate 5;6 top of the screw rod is fixed with stress cup 7;It is described by
7 inside of power cup is placed with collection cups 8;8 top face drain bucket 9 of the collection cups, is screwed on by screw rod 6 on flanged plate, energy
It is enough that stress cup 7 is subjected to lift adjustment, to ensure that collection cups 8 are accurately docked with drain bucket 2.
In another embodiment, 2 its outer diameter of the drain bucket is less than 8 internal diameter of collection cups, can fast implement docking.
In another embodiment, the sorting bin 1 is thirdly compartment is anode sorting compartment 11, cathode sorting compartment 12 and neutrality
Sort compartment 13.
In another embodiment, the anode sorting compartment 11 and cathode sorting compartment 12 are far from neutral sorting side between 13
It has been made into integration sorting and has received and expanded outer 14;The sorting receives expansion outer 14 and is set to immediately below deflecting plates;Sorting connects
The entire bottom surface of deflecting plates can be coated by receiving expansion outer 14, ensure that collection liquid is more comprehensive.
In another embodiment, the anode sorting compartment 11 and cathode 12 top surface of sorting compartment are less than neutral sorting compartment 13
Apical side height, anode sorting compartment 11 and cathode sorting compartment 12 use cladding deflecting plates comprehensively, and by improving neutral sort
13 height of compartment, can avoid neutral solution from entering avris compartment.
In another embodiment, the collection cups 8 are undergauge cup;The lower section at undergauge of the collection cups 8 offers liquid outlet;
The collection cups 8 are provided with water conservancy diversion bucket 81 in liquid outlet lower outside, can avoid enter into dust, and the water conservancy diversion for passing through avris
Bucket can export collection liquid.
In another embodiment, 4 inboard width of liquid collecting frame is more than the width between grip block 3.
In another embodiment, mounting slot 31 is offered on the grip block 3, can be coordinated with flow cytometer and be installed.
In another embodiment, 6 periphery of the screw rod is provided with elastic component 61;61 top of the elastic component is adjacent to flanged plate 5
Bottom surface, 61 bottom of the elastic component are adjacent to screw rod 6 its screw head.
In another embodiment, the stress cup 7, collection cups 8 and 2 central shaft of drain bucket are in same straight line.
Above-described embodiment is only the better embodiment of the present invention, therefore all structures according to described in present patent application range
It makes, the equivalent change or modification that feature and principle are done, is included within the scope of present patent application.
Claims (10)
1. a kind of flow cytometer collector, it is characterised in that:Include the sorting bin of at least three compartments, its is each for the sorting bin
Compartment bottom has been made into integration drain bucket;Two outer avris of the sorting bin is fixed with grip block;The grip block bottom is fixed to
Liquid collecting frame;The liquid collecting frame lower inside is fixed with flanged plate;It is screwed with screw rod on the flanged plate;The screw tip is fixed
There is stress cup;It is placed with collection cups on the inside of the stress cup;Face drain bucket at the top of the collection cups.
2. flow cytometer collector according to claim 1, it is characterised in that:Described its outer diameter of drain bucket, which is less than, to be collected
Cup internal diameter.
3. flow cytometer collector according to claim 1, it is characterised in that:The sorting bin is thirdly compartment is anode
Sort compartment, cathode sorting compartment and neutral sorting compartment.
4. flow cytometer collector according to claim 3, it is characterised in that:The anode sorting compartment and cathode point
It selects compartment to be made into integration sorting far from neutral sorting compartment side and received and expands outer;The sorting, which receives, expands outer setting
Immediately below deflecting plates.
5. flow cytometer collector according to claim 3, it is characterised in that:The anode sorting compartment and cathode point
Select compartment top surface less than neutral sorting compartment apical side height.
6. flow cytometer collector according to claim 1, it is characterised in that:The collection cups are undergauge cup;It is described
Collection cups lower section at undergauge offers liquid outlet;The collection cups are provided with water conservancy diversion bucket in liquid outlet lower outside.
7. flow cytometer collector according to claim 1, it is characterised in that:The liquid collecting frame inboard width is more than folder
Hold the width between plate.
8. flow cytometer collector according to claim 1, it is characterised in that:Mounting is offered on the grip block
Slot.
9. flow cytometer collector according to claim 1, it is characterised in that:The screw rod periphery setting is flexible
Part;It is adjacent to flanged plate bottom surface at the top of the elastic component, the elastic component bottom is adjacent to screw rod its screw head.
10. flow cytometer collector according to claim 1, it is characterised in that:The stress cup, collection cups and drain
The central shaft that struggles against is in same straight line.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201810824986.2A CN108663305A (en) | 2018-07-25 | 2018-07-25 | A kind of flow cytometer collector |
Applications Claiming Priority (1)
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CN201810824986.2A CN108663305A (en) | 2018-07-25 | 2018-07-25 | A kind of flow cytometer collector |
Publications (1)
Publication Number | Publication Date |
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CN108663305A true CN108663305A (en) | 2018-10-16 |
Family
ID=63789505
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CN201810824986.2A Pending CN108663305A (en) | 2018-07-25 | 2018-07-25 | A kind of flow cytometer collector |
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Citations (12)
Publication number | Priority date | Publication date | Assignee | Title |
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GB1264201A (en) * | 1969-03-15 | 1972-02-16 | ||
CN202447107U (en) * | 2012-01-06 | 2012-09-26 | 波音特生物科技(南京)有限公司 | Reagent bottle for detecting serum glucose |
US20120301869A1 (en) * | 2011-05-25 | 2012-11-29 | Inguran, Llc | Particle separation devices, methods and systems |
CN104764676A (en) * | 2015-04-30 | 2015-07-08 | 淮南市产品质量监督检验所 | Small-volume bulk density automatic measurement device for coal |
CN204501434U (en) * | 2015-03-14 | 2015-07-29 | 杨静 | A kind of bone surgery rinses integrated vehicle |
CN105457361A (en) * | 2015-11-16 | 2016-04-06 | 常州大学 | Dual-translation gear transmission device for automatic filtering and cleaning of bituminous coal firing substances |
CN205404208U (en) * | 2016-02-16 | 2016-07-27 | 天津市气象科学研究所 | Fog experimental analysis collector |
CN105833585A (en) * | 2016-05-04 | 2016-08-10 | 武汉工程大学 | Solid-liquid separation vacuum suction filtration device and suction filtration method using same |
CN205861516U (en) * | 2016-08-15 | 2017-01-04 | 合肥安为康医学检验有限公司 | A kind of catcher of flow cytometer |
CN206450547U (en) * | 2016-12-21 | 2017-08-29 | 重庆会通新材料有限公司 | One kind surveys density devices |
CN207197903U (en) * | 2017-07-31 | 2018-04-06 | 中国水产科学研究院南海水产研究所 | A kind of Multifunctional marine planktonic organism sample concentrator and harvester |
CN208568543U (en) * | 2018-07-25 | 2019-03-01 | 暨南大学 | A kind of flow cytometer collector |
-
2018
- 2018-07-25 CN CN201810824986.2A patent/CN108663305A/en active Pending
Patent Citations (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB1264201A (en) * | 1969-03-15 | 1972-02-16 | ||
US20120301869A1 (en) * | 2011-05-25 | 2012-11-29 | Inguran, Llc | Particle separation devices, methods and systems |
CN202447107U (en) * | 2012-01-06 | 2012-09-26 | 波音特生物科技(南京)有限公司 | Reagent bottle for detecting serum glucose |
CN204501434U (en) * | 2015-03-14 | 2015-07-29 | 杨静 | A kind of bone surgery rinses integrated vehicle |
CN104764676A (en) * | 2015-04-30 | 2015-07-08 | 淮南市产品质量监督检验所 | Small-volume bulk density automatic measurement device for coal |
CN105457361A (en) * | 2015-11-16 | 2016-04-06 | 常州大学 | Dual-translation gear transmission device for automatic filtering and cleaning of bituminous coal firing substances |
CN205404208U (en) * | 2016-02-16 | 2016-07-27 | 天津市气象科学研究所 | Fog experimental analysis collector |
CN105833585A (en) * | 2016-05-04 | 2016-08-10 | 武汉工程大学 | Solid-liquid separation vacuum suction filtration device and suction filtration method using same |
CN205861516U (en) * | 2016-08-15 | 2017-01-04 | 合肥安为康医学检验有限公司 | A kind of catcher of flow cytometer |
CN206450547U (en) * | 2016-12-21 | 2017-08-29 | 重庆会通新材料有限公司 | One kind surveys density devices |
CN207197903U (en) * | 2017-07-31 | 2018-04-06 | 中国水产科学研究院南海水产研究所 | A kind of Multifunctional marine planktonic organism sample concentrator and harvester |
CN208568543U (en) * | 2018-07-25 | 2019-03-01 | 暨南大学 | A kind of flow cytometer collector |
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Application publication date: 20181016 |