CN205861516U - A kind of catcher of flow cytometer - Google Patents

A kind of catcher of flow cytometer Download PDF

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Publication number
CN205861516U
CN205861516U CN201620932256.0U CN201620932256U CN205861516U CN 205861516 U CN205861516 U CN 205861516U CN 201620932256 U CN201620932256 U CN 201620932256U CN 205861516 U CN205861516 U CN 205861516U
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China
Prior art keywords
collecting tank
collecting
flow cytometer
catcher
fluid container
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CN201620932256.0U
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Chinese (zh)
Inventor
邵从军
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HEFEI ANWEIKANG MED LAB Co Ltd
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HEFEI ANWEIKANG MED LAB Co Ltd
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Abstract

This utility model provides the catcher of a kind of flow cytometer, relate to flow cytometer field, including circular collecting platform, collecting platform lower end is fixed on base by column, the upper center of described collecting platform is fixed with the waste fluid container of opening upwards, the both sides of waste fluid container are respectively provided with the first collecting tank, the second collecting tank, described first collecting tank, the second collecting tank are arranged in being all bumped into collecting platform, the two sides of described waste fluid container are arc surface, and the lower end of both sides arc surface extends respectively to the first collecting tank, the upper end open of the second collecting tank.This utility model simple in construction, for supporting the use with flow cytometer, can comprehensively collect the drop after separation, there is not waste.

Description

A kind of catcher of flow cytometer
Technical field
This utility model relates to flow cytometer field, particularly relates to the catcher of a kind of flow cytometer.
Background technology
Flow cytometer (Flow cytometer) is the device automatically analyzing cell and sorting.It can be quick Measure, store, show the feature in terms of a series of important biophysics of the cell dispersion floated on a liquid, biochemistry Parameter, it is possible to the cell subsets specified therefrom is sorted out according to the parameter ranges of preliminary election.Flow cytometer is mainly by four Part composition.They are: flow chamber and liquid fluid system;Lasing light emitter and optical system;Photocell and detecting system;Computer and point Analysis system.
Operation principle: flow cytometer can carry out measuring multiple parameters simultaneously, information essentially from specific fluorescence signal and Non-fluorescence scattered signal.Measurement is carried out at measurement zone, and so-called measurement zone is exactly irradiating laser bundle and the liquid stream of ejection spray orifice Bundle intersects vertically a little.When the individual cells of liquid stream central authorities is by measurement zone, can be the whole of 2 π to solid angle by laser irradiation Space scattering light, the wavelength of scattered light is identical with the wavelength of incident illumination.The intensity of scattered light and spatial distribution thereof and cell Size, form, plasma membrane are closely related with cell interior structure because these biological parameters again with cell to the reflection of light, The optical characteristics such as refraction are relevant.The cell being subject to any damage all has distinctive scattering to light, therefore may utilize not Same scattered light signal is to being analyzed without stain living cells and sorting.
The sorting function of flow cytometer is completed by cell sorter.Total process is: the liquid penetrated by nozzle Post is divided into a series of little water droplet, is distinguished whether will be sorted according to certain selected parameter by logic circuit, then by Charging circuit is to selected cell drop charge, and charged drop is carried cell and deflected by electrostatic field, falls in catcher; Other liquid is taken as waste liquid suction to fall.
The existing catcher for flow cytometer, is to be combined by test tube and flask, catches respectively to not Tongfang To the drop separated, but the drop separated, under the effect of electrostatic field, there is fixing detaching direction, but separate the track of whereabouts Affected by many, there is uncertain track, so collecting the drop separated to there is showing of waste with existing catcher As.
Utility model content
The purpose of this utility model is to provide the catcher of a kind of flow cytometer, to solve above-mentioned technical problem.
Technical problem to be solved in the utility model realizes by the following technical solutions:
A kind of catcher of flow cytometer, it is characterised in that: including the collecting platform of circle, collecting platform lower end is passed through Column is fixed on base, and the upper center of described collecting platform is fixed with the waste fluid container of opening upwards, the two of waste fluid container Side is respectively provided with the first collecting tank, the second collecting tank, and described first collecting tank, the second collecting tank set in being all bumped into collecting platform Putting, the two sides of described waste fluid container are arc surface, and the lower end of both sides arc surface extends respectively to the first collecting tank, the second liquid collecting The upper end open of groove.
The centre position, bottom of described waste fluid container is provided with Projecting Lamp, and Projecting Lamp is bumped into the internal setting of collecting platform, throws The light beam that light modulation upwards penetrates, as benchmark, it is simple to the exact placement of catcher.
Described first collecting tank, the second collecting tank lower end are respectively connected with flow-guiding channel, and flow-guiding channel lower end connects test tube.
Being respectively arranged with layering groove in described first collecting tank, the second collecting tank, layering trench bottom is circular arc.Layering groove Use the amount of liquid separated is less when, be directly placed in the first collecting tank and the second collecting tank.
Under the effect of electrostatic field, charged drop separates to waste fluid container both sides respectively, falls at the circle of waste fluid container both sides In cambered surface, drop is snapped down in the first collecting tank or the second collecting tank along arc surface, it is achieved separate.
The drop collected in first collecting tank and the second collecting tank, enters test tube along flow-guiding channel, can use.
The beneficial effects of the utility model are:
This utility model simple in construction, for supporting the use with flow cytometer, can comprehensively collect the liquid after separation Drip, there is not waste.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model.
Detailed description of the invention
For the technological means making this utility model realize, creation characteristic, reach purpose and be easy to understand with effect, under Face with specific embodiments and the drawings, is expanded on further this utility model, but following embodiment is only of the present utility model excellent Select embodiment, and not all.Based on the embodiment in embodiment, those skilled in the art are not making creative work Lower the obtained other embodiments of premise, broadly falls into protection scope of the present invention.
Specific embodiment of the utility model is described below in conjunction with the accompanying drawings.
As it is shown in figure 1, the catcher of a kind of flow cytometer, including circular collecting platform 1, collecting platform 1 lower end leads to Crossing column 8 to be fixed on base 7, the upper center of collecting platform 1 is fixed with the waste fluid container 3 of opening upwards, waste fluid container 3 Both sides are respectively provided with first collecting tank the 9, second collecting tank 4, and first collecting tank the 9, second collecting tank 4 is all bumped in collecting platform 1 Arranging, the two sides of waste fluid container 3 are arc surface, and the lower end of both sides arc surface extends respectively to first collecting tank the 9, second liquid collecting The upper end open of groove 4.
The centre position, bottom of waste fluid container 3 is provided with Projecting Lamp 2, and Projecting Lamp 2 is bumped into the internal setting of collecting platform 1, throws The light beam that light modulation 2 upwards penetrates, as benchmark;
First collecting tank the 9, second collecting tank 4 lower end is respectively connected with flow-guiding channel 5, and flow-guiding channel 5 lower end connects test tube 6.
Of the present utility model ultimate principle, principal character and of the present utility model advantage have more than been shown and described.One's own profession Skilled person will appreciate that of industry, this utility model is not restricted to the described embodiments, described in above-described embodiment and description Be only preference of the present utility model, be not intended to limit this utility model, without departing from this utility model spirit and scope On the premise of, this utility model also has various changes and modifications, and these changes and improvements both fall within this claimed practicality In novel scope.This utility model claims scope and is defined by appending claims and equivalent thereof.

Claims (4)

1. the catcher of a flow cytometer, it is characterised in that: including the collecting platform of circle, collecting platform lower end is by vertical Post is fixed on base, and the upper center of described collecting platform is fixed with the waste fluid container of opening upwards, the both sides of waste fluid container Being respectively provided with the first collecting tank, the second collecting tank, described first collecting tank, the second collecting tank are arranged in being all bumped into collecting platform, The two sides of described waste fluid container are arc surface, and the lower end of both sides arc surface extends respectively to the first collecting tank, the second collecting tank Upper end open.
The catcher of flow cytometer the most according to claim 1, it is characterised in that: in the middle of the bottom of described waste fluid container Position is provided with Projecting Lamp, and Projecting Lamp is bumped into the internal setting of collecting platform, and the light beam that Projecting Lamp upwards penetrates, as benchmark.
The catcher of flow cytometer the most according to claim 1, it is characterised in that: described first collecting tank, the second collection Liquid bath lower end is respectively connected with flow-guiding channel, and flow-guiding channel lower end connects test tube.
The catcher of flow cytometer the most according to claim 1, it is characterised in that: described first collecting tank, the second collection Being respectively arranged with layering groove in liquid bath, layering trench bottom is circular arc.
CN201620932256.0U 2016-08-15 2016-08-15 A kind of catcher of flow cytometer Active CN205861516U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201620932256.0U CN205861516U (en) 2016-08-15 2016-08-15 A kind of catcher of flow cytometer

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201620932256.0U CN205861516U (en) 2016-08-15 2016-08-15 A kind of catcher of flow cytometer

Publications (1)

Publication Number Publication Date
CN205861516U true CN205861516U (en) 2017-01-04

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Country Status (1)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106872342A (en) * 2017-04-13 2017-06-20 江苏省血吸虫病防治研究所 A kind of quick filter for preparing single cell suspension
CN108663305A (en) * 2018-07-25 2018-10-16 暨南大学 A kind of flow cytometer collector
CN112136034A (en) * 2018-04-27 2020-12-25 贝克顿·迪金森公司 Collection system for flow cytometry sorting samples and methods of use thereof

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106872342A (en) * 2017-04-13 2017-06-20 江苏省血吸虫病防治研究所 A kind of quick filter for preparing single cell suspension
CN112136034A (en) * 2018-04-27 2020-12-25 贝克顿·迪金森公司 Collection system for flow cytometry sorting samples and methods of use thereof
CN108663305A (en) * 2018-07-25 2018-10-16 暨南大学 A kind of flow cytometer collector

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