CN108660053A - A kind of body eutrophication indicating means based on micro-fluidic chemotactic chip - Google Patents
A kind of body eutrophication indicating means based on micro-fluidic chemotactic chip Download PDFInfo
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Abstract
The present invention provides a kind of micro-fluidic chemotactic chip, and there are three units to constitute for the chip, and nutrition water body gradient forms unit, cell sample injection unit and chemotactic effect sight chamber；Wherein, nutrition water body gradient forms unit, can form one kind of nutrition water body line source or point source gradient；Cell sample injection unit is located at corresponding position according to gradient generation type.The present invention also provides a kind of body eutrophication indicating means based on micro-fluidic chemotactic chip, have the advantages that：Micro-fluidic chemotactic chip can accurately simulate nutrition water body line source and point source gradient, and friendly test platform is provided to the chemotactic of nutriment for microalgae and bacterium；It is more convenient, intuitive to carry out the evaluation of water body eutrophication degree from finding out for biological phenomenon itself rather than physicochemical environment, to provide supplement based on the Evaluation Methods For Eutrophication that physicochemical environment is found out to be existing.
Description
Technical field
The present invention relates to microfluidic chip technologies and aquatic ecological environmental evaluation field, more particularly to one kind is based on micro-fluidic
The body eutrophication indicating means of chemotactic chip.
Background technology
Different signs indicate, and the biology of the bioactive elemements such as carbon on the earth, nitrogen, sulphur, phosphorus is being interfered or changing in mankind's activity
Geochemical cycle leads to a series of serious ecological effects and environmental degradation.Wherein body eutrophication has become puzzlement generation
One of the main Environmental Problems of various countries of boundary.Many researchers study Evaluation of Eutrophication, and propose a variety of rich battalion
Fosterization evaluation model.Wherein, the nutrient concentrations such as nitrogen, phosphorus are still the most important monitoring of current seawater eutrophication and comment in seawater
Valence index is still the most successful method of the early warning of harmful algal bloom finding out for the physicochemical environment to algal tufa may occur, and
The non-characterization to algal tufa itself.But since the measurement of the parameters such as nutritive salt, COD needs relatively large instrument and equipment and there is analysis week
The features such as phase length, heavy workload.And then bring Evaluation of Eutrophication model into, carry out risk estimation.From the time, workload and
On analysis cost, it is unfavorable for the quick monitoring and evaluation of body eutrophication, it is even more impossible to timely early warning.
Chemotactic is the main dynamic behavior of marine microalgae and bacterium, refers to the concentration of bacterium and microalgae to a variety of chemical substances
Tend to caused by gradient or is reacted from keeping away.Physico chemical factor is complicated and uneven in natural marine site, when nutritious substances accumulation occurs
Relative concentration is still very low, and diffusion length is limited (only several centimeters), and the duration is very short (10-15min), is needed to survive
It wants, planktonic microalgae and bacterium must have extremely strong recognition capability and rapid locomotivity, to be exposed to the maximum extent
In limited nutritional resource.Still there is extremely strong knowledge to the nutriment in complicated marine environment in view of marine microalgae and bacterium
Other ability, chemotactic induction are sensitive, rapid.Therefore, pass through detection bacterium and microalgae chemotactic behavior whether generation, chemotactic speed
Speed can provide scientific basis with the nourishment of Efficient Characterization seawater for Environment Management of Eutrophication and estimating for red tide.
Chemotactic analysis for nutriment, is characterized by water body in-situ environment and accurately simulates, stable, favorable reproducibility
Nutriment line source and point source gradient are formed and what the real-time online of microalgae chemotactic behavior was observed integrates.Simultaneously, it is contemplated that be based on
Quickly detection and emergent, early warning and monitoring testing requirement, constructed test system must have both that high-throughput, integrated, price is low
The features such as honest and clean and adaptation scene.These are difficult to realize for current conventional method.The appearance of microfluidic chip technology is solution
Certainly the above problem provides new opportunity.Microfluid accurately control be micro-fluidic chip notable feature, the essence of chemical gradient
True structure is that flexibly control is micro-fluidic chip significant advantage place based on microfluid.In recent years, researcher has developed greatly
Free-flowing, shape can be generated arbitrarily and the gradient of gradient stabilization generates chip and is used for chemical gradient in simulating natural environment
Space-time generates and evanishment.
Invention content
The purpose of the present invention is to provide a kind of body eutrophication indicating means based on micro-fluidic chemotactic chip, specifically
It is to utilize microfluidic chip technology, friendly test platform is provided to the chemotactic of nutriment for microalgae and bacterium, accurately to refer to
Show water nutrition degree, from finding out for biological phenomenon itself rather than physicochemical environment, to carry out commenting for water body eutrophication degree
Valence.To provide supplement based on the Evaluation Methods For Eutrophication that physicochemical environment is found out to be existing, while it being water nutrition situation
Quick detection, red tide, bloom prealarming etc. provide a kind of quick, convenient, suitable for scene method.
The present invention using following technical scheme in order to solve the above technical problems, realized：
A kind of micro-fluidic chemotactic chip, it is characterised in that：There are three units to constitute for the chip, and nutrition water body gradient forms list
Member, cell sample injection unit and chemotactic effect sight chamber；Wherein, nutrition water body gradient forms unit, can form nutrition water body line
One kind of source or point source gradient；Cell sample injection unit is located at corresponding position according to gradient generation type.
Preferably, the nutrition water body line source gradient forms unit, and it is to be made of upstream trident " Ψ " type channel, both sides
Channel connects and composes cell sample injection unit with cell injection port, and intermediate channel is that nutrition water body enters channel；Triple channel fluid is simultaneously
Stream forms nutrition water body line source in central axes, flows into downstream chemotactic effect sight chamber, is eventually connected to waste liquid pool.
Preferably, the nutrition water body point source gradient forms unit, it is by nutrition water body liquid storage tank, sample intake passage and expansion
Scatterplot is constituted, and liquid storage tank connects sample intake passage, and sample intake passage end is by spreading point and chemotactic effect sight chamber one end central axes
Connection；Cell sample injection unit is made of cell liquid storage tank and narrow thin channel, by narrow thin channel and the gradient culturing room other end
Axis connects.
Preferably, entire flow path channel height is 50 μm, both sides channel width is equal in " Ψ " the type channel of upstream is
300~500 μm, length 1cm；150~250 μm of intermediate channel width, length 1cm；Two wing passages and intermediate channel angle
It is 45 °；Chemotactic effect sight chamber width is 2cm, length 4cm.
Preferably, being made of upper layer and lower layer, upper layer is fluid path layer, and lower layer is blanket layer.
Preferably, entire flow path channel height is 50 μm.Nutrition water body and cell liquid storage tank radius are 1~2mm, deep
Degree is 2~4mm；Sample intake passage width is 200~300 μm, length 1cm；The narrow thin channel width of cell sample introduction is 50~100
μm, length 1cm；Gradient culture observation ward width is circle, radius 2cm.Diffusion point is that semi-permeable membrane (includes but are not limited to：
Polycarbonate membrane, nitrocellulose filter, cellulose acetate film) or hydrogel (include but are not limited to, sodium alginate, polypropylene
Amide, chitosan, collagen, polyvinyl alcohol).
Preferably, when diffusion point is semi-permeable membrane, which is made of upper layer and lower layer, and nutrition water body sample intake passage is located at
Chip lower layer is connect by semi-permeable membrane with chip upper layer chemotactic effect sight chamber, and cell sample intake passage also is located at chip upper layer.
Preferably, when diffusion point is hydrogel, which is made of upper layer and lower layer, and upper layer is fluid path layer, and lower layer is
Blanket layer.Hydrogel blocks in nutrition water body sample intake passage end, and channel is connect with chemotactic effect sight chamber.
Preferably, its material can be one kind of glass, PDMS and PMMA.It is irreversible using glass chemistry etching and high temperature
A kind of structure in burn-back, PDMS Soft lithographs and the irreversible sealing-in of plasma and PMMA hot pressing and hot-press sealing technology.
A kind of body eutrophication indicating means based on micro-fluidic chemotactic chip, it is characterised in that：Pass through the channel " Ψ "
Liang Ge collateral branch pipeline conveys cell to chemotactic effect detection room；Central nutrition water body sample intake passage is conveyed to chemotactic effect detection room
Solution containing nutriment.In central nutrients under micro-meter scale is logical fluid present laminar flow characteristic (reynolds number Re~
0.01) laminar flow zone, is formed in chemotactic effect detection area.After fluid shutoff, by diffusion, in chemotactic channel certainly
It is dynamic to generate the nutrients line source concentration gradient successively decreased by mediad channel both sides, cell after incude nutrients, by two laterally in
Between move about, indicate water nutrition feature.With chemotactic effect occur with whether indicate the presence or absence of nutriment.By hungry or special
After nutritional condition induces (including but are not limited to glycine, Ala, Asp, Glu, Met, nitrate, urea is only nitrogen source)
Cell can generate chemotactic to specific nutrition substance, indicate nutriment type.
A kind of body eutrophication indicating means based on micro-fluidic chemotactic chip, it is characterised in that：Nutrition water body liquid storage
Water body in pond and sample intake passage is obstructed by diffusion point (one kind of semi-permeable membrane or hydrogel) and chemotactic effect sight chamber, only nutrition
Substance can be diffused into chemotactic effect sight chamber by spreading point, form nutriment point source diffusion gradient.Cell culture chamber it is thin
Born of the same parents are moved after incuding nutrients from culturing room to nutriment point source, indicate water nutrition feature.When being continued with chemotactic effect
Between length instruction nutrient concentrations height.By the induction of hungry or special dietary condition (include but are not limited to glycine,
Ala, Asp, Glu, Met nitrate, urea are only nitrogen source) cell can be to specific nutrition substance generation chemotactic effect, instruction afterwards
Nutriment type.
Preferably, the mode that the chemotactic effect characterization may be used CCD video recordings or take pictures, analyzes algae from image
The ratio of gray scale or fluorescence intensity before occurring relative to chemotactic under chemotactic high density state.
Preferably, the cell is one kind with amphitrichous, the aquatic bacteria that can be moved about or microalgae.
The beneficial effects of the invention are as follows：
(1) micro-fluidic chemotactic chip can accurately simulate nutrition water body line source and point source gradient, be microalgae and bacterium to battalion
The chemotactic for supporting substance provides friendly test platform；
(2) finding out from biological phenomenon itself rather than physicochemical environment, to carry out the evaluation of water body eutrophication degree, more
For convenience, intuitive, to provide supplement based on the Evaluation Methods For Eutrophication that physicochemical environment is found out to be existing；
(3) this method have the characteristics that it is quick, easy to operate, without large-scale instrument, suitable for scene, can be water nutrition feelings
The quick detection of condition, red tide, bloom prealarming etc. providing method.
Description of the drawings
Fig. 1 is can to generate nutriment line source gradient chemotactic chip and cell generation chemotactic effect schematic diagram；
Fig. 2 is can to generate nutriment point source gradient chemotactic chip and cell generation chemotactic effect schematic diagram；
Specific implementation mode
In order to make the technical means, the creative features, the aims and the efficiencies achieved by the present invention be easy to understand, tie below
Specific embodiments and the drawings are closed, the present invention is further explained, but following embodiments are only the preferred embodiment of the present invention, not
All.Based on the implementation example in the implementation mode, those skilled in the art are obtained without making creative work
Other embodiments belong to protection scope of the present invention.
Specific embodiments of the present invention are described below in conjunction with the accompanying drawings.
As shown in Figure 1 and Figure 2,
Micro-fluidic chemotactic chip provided in this embodiment can form nutrition water body line source gradient.
Such as Fig. 1, shown in Fig. 1：1. nutrition water body gradient forms unit；2. chemotactic effect sight chamber；3. cell injection port；
4. cell sample intake passage；5. nutrition water body sample intake passage；6. waste liquid port.
The micro-fluidic chemotactic chip for forming line source nutrition water body is imitated by the chemotactic in upstream trident " Ψ " type channel and downstream
Sight chamber is answered to constitute.Two wing passage of upstream connects and composes cell sample injection unit with cell injection port, and intermediate channel is nutrition water body
Into channel.Triple channel fluid cocurrent forms nutrition water body line source in central axes, flows into downstream chemotactic effect sight chamber, finally connects
It is connected to waste liquid pool.
The micro-fluidic chemotactic chip of line source nutrition water body is formed, the entire flow path channel height of the chip is 50 μm.Upstream
It is 300~500 μm that both sides channel width is equal in " Ψ " type channel, length 1cm；150~250 μm of intermediate channel width,
Length is 1cm；Two wing passages and intermediate channel angle are 45 °；Chemotactic effect sight chamber width is 2cm, length 4cm.
Micro-fluidic chemotactic chip provided in this embodiment can form nutrition water body point source gradient.
As shown in Fig. 2, in figure：1. nutrition water body liquid storage pool；2. nutrition water body sample intake passage；3. chemotactic effect sight chamber；
4. diffusion point；5. cell sample intake passage；6. cell liquid storage pool.
The micro-fluidic chemotactic chip for forming point source nutrition water body is by nutrition water body liquid storage tank, sample intake passage, diffusion point, becomes
Change effect sight chamber and cell sample injection unit is constituted.
The micro-fluidic chemotactic chip of point source nutrition water body is formed, the entire flow path channel height of the chip is 50 μm.Nutritive water
Body and cell liquid storage tank radius are 1~2mm, and depth is 2~4mm；Sample intake passage width is 200~300 μm, length 1cm；
The narrow thin channel width of cell sample introduction is 50~100 μm, length 1cm；Gradient culture observation ward width is circle, radius 2cm.
Diffusion point is that semi-permeable membrane (includes but are not limited to：Polycarbonate membrane, nitrocellulose filter, cellulose acetate film) or hydrogel
(including but are not limited to, sodium alginate, polyacrylamide, chitosan, collagen, polyvinyl alcohol).
Embodiment 1
Dunaliella salina is after hungry preculture, with external Micropump by the channel " Ψ " Liang Ge collateral branch pipeline to chemotactic effect
Sensing chamber conveys, and ammonium salt-containing (NH4Cl) seawater is with external Micropump by central nutrition water body sample intake passage to chemotactic effect detection
Room conveys.Laminar flow characteristic (reynolds number Re is~0.01) is presented in the logical interior fluid of central nutrients under micro-meter scale, is imitated in chemotactic
It answers and forms laminar flow zone in detection zone.After fluid shutoff, by diffusion, automatically generated by mediad in chemotactic channel
The nutrients line source concentration gradient that channel both sides are successively decreased, Dunaliella salina is after incuding nutrients, by two lateral intermediate travellings.Ammonium salt
When a concentration of 1 μM of -1mA, chemotactic effect is generated in 1-2min, chemotactic effect degree is 4~4.5.
Embodiment 2
Ancient card shield algae is after hungry preculture, with external Micropump by the channel " Ψ " Liang Ge collateral branch pipeline to chemotactic effect
Sensing chamber conveys, and ammonium salt-containing (NH4Cl) seawater is with external Micropump by central nutrition water body sample intake passage to chemotactic effect detection
Room conveys.Laminar flow characteristic (reynolds number Re is~0.01) is presented in the logical interior fluid of central nutrients under micro-meter scale, is imitated in chemotactic
It answers and forms laminar flow zone in detection zone.After fluid shutoff, by diffusion, automatically generated by mediad in chemotactic channel
The nutrients line source concentration gradient that channel both sides are successively decreased, Dunaliella salina is after incuding nutrients, by two lateral intermediate travellings.Ammonium salt
When a concentration of 0.2 μM of -1mA, chemotactic effect is generated in 1-2min, chemotactic effect degree is 5~6.
Embodiment 3
Water body in nutrition water body liquid storage tank and sample intake passage is by diffusion point polyvinyl alcohol gel and chemotactic effect sight chamber
Barrier, 100 μM of nutriment can be diffused into chemotactic effect sight chamber by spreading point, form nutriment point source diffusion ladder
Degree.It will be put into cell culture chamber by the hidden algae of orchid of amino acid Fiber differentiation, when nutriment is Ala, Asp, Glu, the ammonia such as Met
It is 3~4 that chemotactic effect degree is generated when base acid, in 2~3min.
Embodiment 4
Water body in nutrition water body liquid storage tank and sample intake passage is hindered by diffusion point polycarbonate membrane with chemotactic effect sight chamber
Every 100 μM of nutriment can be diffused into chemotactic effect sight chamber by spreading point, form nutriment point source diffusion gradient.
It will be put into cell culture chamber by the hidden algae of orchid of amino acid Fiber differentiation, when nutriment is NH4Cl, does not generate chemotactic effect
It answers.
The basic principles, main features and advantages of the present invention have been shown and described above.The technology of the industry
For personnel it should be appreciated that the present invention is not limited to the above embodiments, described in the above embodiment and specification is only the present invention
Preference, be not intended to limit the present invention, without departing from the spirit and scope of the present invention, the present invention also has various
Changes and improvements, these changes and improvements all fall within the protetion scope of the claimed invention.The claimed scope of the invention is by institute
Attached claims and its equivalent thereof.
Claims (14)
1. a kind of micro-fluidic chemotactic chip, it is characterised in that：There are three units to constitute for the chip, and nutrition water body gradient forms list
Member, cell sample injection unit and chemotactic effect sight chamber；Wherein, nutrition water body gradient forms unit, can form nutrition water body line
One kind of source or point source gradient；Cell sample injection unit is located at corresponding position according to gradient generation type.
2. micro-fluidic chemotactic chip according to claim 1, it is characterised in that：The nutrition water body line source gradient forms list
Member is made of upstream trident " Ψ " type channel, and two wing passages connect and compose cell sample injection unit with cell injection port, and centre is logical
Road is that nutrition water body enters channel；Triple channel fluid cocurrent forms nutrition water body line source in central axes, flows into downstream chemotactic effect
Sight chamber is eventually connected to waste liquid pool.
3. micro-fluidic chemotactic chip according to claim 1, it is characterised in that：The nutrition water body point source gradient forms list
Member is made of nutrition water body liquid storage tank, sample intake passage and diffusion point, and liquid storage tank connects sample intake passage, and sample intake passage end is logical
Diffusion point is crossed to connect with chemotactic effect sight chamber one end central axes；Cell sample injection unit is by cell liquid storage tank and narrow thin channel structure
At being connect with gradient culturing room other end central axes by narrow thin channel.
4. micro-fluidic chemotactic chip according to claim 1 or 2, it is characterised in that：Entire flow path channel height is 50 μm.
It is 300-500 μm that both sides channel width is equal in " Ψ " the type channel of upstream, length 1cm；Intermediate channel width 150-250 μ
M, length 1cm；Two wing passages and intermediate channel angle are 45 °；Chemotactic effect sight chamber width is 2cm, length 4cm.
5. micro-fluidic chemotactic chip according to claim 1 or 2, it is characterised in that：It is made of upper layer and lower layer, upper layer is liquid
Road floor, lower layer are blanket layer.
6. micro-fluidic chemotactic chip according to claim 1 or 3, it is characterised in that：Entire flow path channel height is 50 μm；
Nutrition water body and cell liquid storage tank radius are 1-2mm, depth 2-4mm；Sample intake passage width is 200-300 μm, and length is
1cm；The narrow thin channel width of cell sample introduction is 50-100 μm, length 1cm；Gradient culture observation ward width is circle, radius
2cm；Diffusion point is semi-permeable membrane or hydrogel.
7. micro-fluidic chemotactic chip according to claim 6, it is characterised in that：When diffusion point be semi-permeable membrane when, the chip by
Upper layer and lower layer is constituted, and nutrition water body sample intake passage is located at chip lower layer, passes through semi-permeable membrane and chip upper layer chemotactic effect sight chamber
Connection, cell sample intake passage also are located at chip upper layer.
8. micro-fluidic chemotactic chip according to claim 6, it is characterised in that：When diffusion point be hydrogel when, the chip by
Upper layer and lower layer is constituted, and upper layer is fluid path layer, and lower layer is blanket layer；Hydrogel blocks in nutrition water body sample intake passage end, will lead to
Road is connect with chemotactic effect sight chamber.
9. according to any one of right 1-3 and the 7-8 micro-fluidic chemotactic chip, it is characterised in that：Its material is glass, PDMS
With one kind in PMMA；Using glass chemistry etching and the irreversible burn-back of high temperature, PDMS Soft lithographs and the irreversible sealing-in of plasma
With a kind of structure in PMMA hot pressing and hot-press sealing technology.
10. a kind of body eutrophication indicating means based on micro-fluidic chemotactic chip described in claim 1, feature exist
In：By " Ψ " type channel Liang Ge collateral branch pipeline cell is conveyed to chemotactic effect detection room；Central nutrition water body sample intake passage to
Chemotactic effect detection room conveys the solution containing nutriment；It is special that laminar flow is presented in the logical interior fluid of central nutrients under micro-meter scale
Sign, reynolds number Re form laminar flow zone~0.01 in chemotactic effect detection area；After fluid shutoff, by diffusion,
The nutrients line source concentration gradient successively decreased by mediad channel both sides is automatically generated in chemotactic channel, cell is in induction nutrients
Afterwards, by two lateral intermediate travellings, water nutrition feature is indicated；With chemotactic effect occur with whether indicate the presence or absence of nutriment；
Cell can generate chemotactic to specific nutrition substance after the induction of hungry or special dietary condition, indicate nutriment type.
11. a kind of body eutrophication indicating means based on micro-fluidic chemotactic chip described in claim 1, feature exist
In：Water body in nutrition water body liquid storage tank and sample intake passage is obstructed by diffusion point with chemotactic effect sight chamber, and only nutriment can
It is diffused into chemotactic effect sight chamber by spreading point, forms nutriment point source diffusion gradient；The cell of cell culture chamber is being felt
It after answering nutrients, is moved from culturing room to nutriment point source, indicates water nutrition feature；With chemotactic duration of effect length
Indicate nutrient concentrations height；Cell can generate chemotactic to specific nutrition substance after the induction of hungry or special dietary condition
Effect indicates nutriment type.
12. the body eutrophication indicating means based on micro-fluidic chemotactic chip according to claim 10 or 11, special
Sign is：The special dietary condition includes but are not limited to
Only nitrogen source.
13. the body eutrophication indicating means based on micro-fluidic chemotactic chip according to claim 10 or 11, special
Sign is：The chemotactic effect characterization is analyzed from image under algae chemotactic high density state in such a way that CCD records a video or takes pictures
The ratio of gray scale or fluorescence intensity before occurring relative to chemotactic.
14. the body eutrophication indicating means based on micro-fluidic chemotactic chip according to claim 10 or 11, special
Sign is：The cell is one kind with amphitrichous, the aquatic bacteria that can be moved about or microalgae.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114164078A (en) * | 2021-11-30 | 2022-03-11 | 齐齐哈尔大学 | Bacterial chemotactic substance screening glass slide and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1992015703A1 (en) * | 1991-03-04 | 1992-09-17 | United States Department Of Energy | Chemotactic selection of pollutant degrading soil bacteria |
| JP2008542702A (en) * | 2005-05-26 | 2008-11-27 | シムトックス リミテッド | Water monitoring system |
| CN103468569A (en) * | 2012-06-08 | 2013-12-25 | 中国科学院大连化学物理研究所 | Micro fluidic chip system for microalgae toxicity test |
| CN104531504A (en) * | 2014-12-16 | 2015-04-22 | 大连理工大学 | Micro-fluidic chip device for separating bacteria by using laminar flow and chemotaxis effects |
| CN104818200A (en) * | 2015-03-16 | 2015-08-05 | 中国科学院微生物研究所 | Sorting device and taxis object sorting method |
-
2018
- 2018-03-13 CN CN201810249656.5A patent/CN108660053A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1992015703A1 (en) * | 1991-03-04 | 1992-09-17 | United States Department Of Energy | Chemotactic selection of pollutant degrading soil bacteria |
| JP2008542702A (en) * | 2005-05-26 | 2008-11-27 | シムトックス リミテッド | Water monitoring system |
| CN103468569A (en) * | 2012-06-08 | 2013-12-25 | 中国科学院大连化学物理研究所 | Micro fluidic chip system for microalgae toxicity test |
| CN104531504A (en) * | 2014-12-16 | 2015-04-22 | 大连理工大学 | Micro-fluidic chip device for separating bacteria by using laminar flow and chemotaxis effects |
| CN104818200A (en) * | 2015-03-16 | 2015-08-05 | 中国科学院微生物研究所 | Sorting device and taxis object sorting method |
Non-Patent Citations (2)
| Title |
|---|
| HONG II CHOI等: "《Quantitative analysis of the chemotaxis of a green alga, Chlamydomonas reinhardtii, to bicarbonate using diffusion-based microfluidic device》", 《BIOMICROFLUIDICS》 * |
| 王玉敏: "《环境水力学》", 28 February 2017, 东南大学出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114164078A (en) * | 2021-11-30 | 2022-03-11 | 齐齐哈尔大学 | Bacterial chemotactic substance screening glass slide and application thereof |
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