CN108651444A - A kind of iris pollen preserves and breeding method - Google Patents
A kind of iris pollen preserves and breeding method Download PDFInfo
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- CN108651444A CN108651444A CN201810496046.5A CN201810496046A CN108651444A CN 108651444 A CN108651444 A CN 108651444A CN 201810496046 A CN201810496046 A CN 201810496046A CN 108651444 A CN108651444 A CN 108651444A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01D—HARVESTING; MOWING
- A01D93/00—Harvesting apparatus not provided for in other groups of this subclass
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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Abstract
The invention discloses a kind of preservation of iris pollen and breeding methods.The present invention all removes the anther cap of iris pollen, pollen load handle and glutinous disk, it is sticked in pollen collecting box using the viscosity of glutinous disk, classification preservation is carried out according to different cultivars after marking, the same day uses or refrigerates and can save two months in refrigerator, 20 DEG C of freezen protective several months.Seedling field cultivation stage after hybridization induced flowering in 2.5 cun of cups selects the seedling that inflorescence is good, pattern meets breeding objective and carries out fast numerous, shortens seedling breeding time 5 months.Fast numerous rear test seedling that obtains is colonized to after greenhouse, start flower forcing after seed in 1.7 cun of plantations of cup seedling, 2.8 cun of cups is planted, Post flowering carries out specificity, consistency, stability test, can also reduce by 5 months or so time, whole flow process shortens the breeding time limit of iris new varieties.
Description
Technical field
The present invention relates to a kind of preservation of iris pollen and breeding methods, belong to technical field of flower breeding.
Background technology
The iris florescence is long, pattern is abundant and gorgeous colourful, is danced in the air like butterfly, there is the good reputation of tropical orchid " queen ", deep
By countries in the world, people are liked, have become one of most important pot flowers in the whole world at present.China is the source area of iris
One of, Taiwan just sets up the status at world iris breeding center early in the 1980s.It is fast by nearly 30 years
Speed development, China mainland area is one of with also becoming the important iris production and consumption in the whole world.But since breeding work is started to walk
Evening, the cultivation for accelerating autonomous kind are the very urgent work of continent iris breeding people.
Pollen bonding in the colored anther of iris every is at two pollen loads, when hybridization, to acquire the flower as male parent
Pollen grain is placed on and removes maternal pollen grain again later as maternal gynostemium intracavitary by powder.At present in crossing work
The problem is that；
1, iris crossing work is inefficient
When carrying out iris hybridization every time, need between running in the guide different Parents, butterfly orchid variety is more, parent material
Also more, generally require to occupy larger position when being hybridized, Parent distance farther out when walk back and forth and slattern many times.
Although the iris florescence is longer, the Pistil And Stamen cross compatibility best time is different, and Pistil recipient phase is preferably opened in flower
In 10-15 days put, pollen activity preferably in flowering 1-7 days (analogy orchid etc., 2017), therefore how when effective
Interior completion crossing work rapidly and efficiently improves the problem of iris hybridization efficiency is urgent need to resolve.
2, without effective iris pollen preserving type
Iris is after hybridization, and the pollen load for needing removal maternal, many pollen can be wasted by directly throwing away, and pollen cannot
When effectively preserving, needs also to need to go again when the pollen of the female parent to acquire, also affect hybridization efficiency；And get rid of pollen load
Iris flower will wilt for second day, it is also difficult to be re-used as female parent and be hybridized, therefore effective iris pollen preservation side
Precious iris germ plasm resource service efficiency can be improved in method.
Since iris pollen grain is smaller, each pollen is required for carrying out seal operation when classification preserves, and is otherwise easy
Pollen is caused to mix, pollen classification preserves work too complex, therefore pollen substantially now enchashment is used when iris hybridization at present.
3, the breeding process period is long
Following process is generally taken in current iris crossbreeding：Hybridization--2.5 cun of -1.5 cun of aseptic seeding cup seedling
- 3.5 cun of cup seedlings of seedling--3.5 cun of cup seedlings of seedling in sterile expansion -2.5 cun of cups of numerous -1.5 cun of cup seedlings of test seedling of flower forcing-- are urged in cup
Flower carries out kind test, and whole process needs 50-55 months.After picking out outstanding seedling, since iris must pass through
Vegetative propagation is proliferated, it is therefore desirable to carrying out proliferation expansion again after inducing aseptic seedling after the progress explant disinfection of outstanding single plant
Numerous, until the microcommunity for breeding out 200-300 plants or so carries out field planting test, character is excellent, market is approved to carry out
The application of kind power and large-scale production.Since iris is supplied mainly as annual flower, breeder would generally adjust seedling
Flowering time, unchecked iris seedling can be sold directly as variegated cost.Therefore iris breeding cycle is often 6
Year or so can just select one can marketing kind.Breeding efficiency how is improved, shortens breeding process, is that iris is educated
The problem of kind urgent need to resolve.
Invention content
The present invention overcomes above-mentioned the deficiencies in the prior art, provides a kind of iris pollen and preserve and breeding method.This
Invention all removes the anther cap of iris pollen, pollen load handle and glutinous disk, and pollen collecting is sticked to using the viscosity of glutinous disk
In box, classification preservation is carried out according to different cultivars after marking, the same day uses or refrigerates can save two in refrigerator
Month, -20 DEG C of freezen protective several months.Seedling field cultivation stage after hybridization induced flowering in 2.5 cun of cups, selects inflorescence
The seedling that good, pattern meets breeding objective carries out fast numerous, shortening seedling breeding time 5 months.It is fast it is numerous after obtain test it is small
Seedling field planting to behind greenhouse, starting flower forcing after seed in 1.7 cun of plantations of cup seedlings, 2.8 cun of cups is planted, Post flowering carry out specificity,
Consistency, stability test, can also reduce by 5 months or so time, and whole flow process shortens the breeding of iris new varieties
The time limit.
The technical scheme is that：A kind of iris pollen store method, characterized in that
1) pollen collection
When iris pollen collection, anther cap, pollen load handle and glutinous disk are all removed, are sticked to using the viscosity of glutinous disk
In pollen collecting box, and preserved after marking；
2) preservation of pollen
If pollen uses in 1-7 days, kept at 18-25 DEG C；
If pollen uses in -2 months 8 days, pollen collecting box is placed in 4 DEG C of refrigerator and is preserved；
If pollen uses after 2 months, pollen collecting box is stored in -20 DEG C of freezing chamber；When freezen protective
Box is encased with valve bag, is vacuumized, polybag is made to be tightly attached on pollen collecting box；When use：By pollen collecting box at 20 DEG C
Naturally to thaw under environment, when pollen thaw and it is consistent with ambient temperature when use.
The present invention also provides a kind of with the matching used iris pollen collecting box of above-mentioned store method, including box body and
Box cover, box cover cover on box body, characterized in that at least 1 layer of the box body, and each layer box body is stacked together；Every layer of box body
Interior to be equipped with multiple grooves for placing iris pollen (anther cap, pollen load handle and glutinous disk), the side of groove, which is provided with, places mark
The label bag of label.
Further, it is provided with buckle on 2 sides or 4 sides of the box cover and bottom box body, it will by buckling
Box cover and bottom box body link together, and intermediate box body is clipped between bottom box body and box cover.Or all box covers and box
Body side is fixed together (such as by the hinge with rotary shaft), and the opposite other side is connected by buckle, magnet etc.
It connects.
Further, it is provided with handle on the box cover, be convenient for carrying.
Further, the length of the buckle can be adjusted, in order to adjust the number of plies of box body.For example the length buckled is most
It is short when being 2 layers of box body；When box body is adjusted to 3 layers, buckle length adjustment (can once be stretched) and be continuing with.
Preferably, 10-20 groove is equipped in the box body.
Preferably, the high 2cm of every layer of box body；Groove size is 3cm × 3cm, deep 0.5cm.
The invention also discloses a kind of iris breeding methods, characterized in that
1) iris hybridizes
Takes powder to be placed in collection box in advance the male parent before iris hybridization, then go directly to plot where maternal plant into
Row hybridization；
The flower of maternal plant is collected using collection box after the pollination for completing paternal pollen, makes a record preservation,
Pollen is provided when doing other hybridization as male parent to use；
For the kind of part flowering asynchronism, pollen is preserved, hybridization is remake when the blossom needed；
2) breeding
It harvests hybrid seed and carries out aseptic seeding, flower forcing processing is carried out when growing to 2.5 cun of cups of middle seedling, it is ornamental according to flower
Character selects suitable kind；
It is excellent for the trait expression of blooming of seedling in 2.5 cun of cups, it blooms without 3.5 cun of seedlings, detects virus-free plant
Direct cut-flower stalk carries out sterile mitogenetic fast numerous, the test group of 300 ± 20 plants of formation；
After seedling field planting to greenhouse to be tested, carries out seedling through 1.7 cun of cups and plant 4 months, seed plants 4 in 2.8 cun of cups
Start flower forcing after month, Post flowering carries out consistency, stability, the specific test of new varieties.
The flower forcing mode is：25-28 DEG C of day temperature, 18-20 DEG C of night temperatures, intensity control is in 20000-
25000lux。
Further, 1-2 months before flower forcing processing, using 1500-2000 times of 10-30-20 nitrogen-phosphorus-potassium compound fertilizer into
Row fertilising, alternately, i.e. hereafter a rich water, a clear water pour clear water i.e. every time until the 1st is spent in full bloom for rich water, clear water
It can.
The beneficial effects of the invention are as follows：
1, hybridization efficiency is improved
It is found in hybridization, iris pollen grain is rolled into a ball by pollen handle and glutinous disk constitutes an entirety, and sticking disk has very by force
Viscosity, can be sticked on object moment, if keeping glutinous disk when taking pollen grain, so that it may pollen is fixed, carry out mark
It can be preserved for a long time after note.
The present invention is collected iris pollen, can facilitate access when can not be completed hybridization within one day, need not answer
Miscellaneous pollen classification save set, and ensure that pollen is vibrant；Powder can also be taken to be placed in collection box in advance male parent simultaneously,
Then the plot where going directly to maternal plant is hybridized, and need not be run in the guide between male parent plot and maternal plot, to carry
High efficiency.
The present invention can also be preserved as the maternal pollen of hybridization, for the male parent as other cross combinations, be reduced not
Necessary pollen waste, improves hybridization efficiency.
The present invention simply and easily preserves iris pollen, flowering asynchronism can may be implemented with freezen protective when needing
Iris hybridizes.
2, shorten the time of seedling seed selection, and then shorten breeding process
Applicant has found in the practices of breeding, when 2.5 cun of cups of iris, as long as giving specific environmental condition, and same energy
Flower is outputed, the present invention observes filial generation phenotype in 2.5 cun of cups (middle seedling stage) flower forcing, can shorten the seed selection time, meanwhile,
The outstanding single plant selected can enter sterile expansion numerous stage in advance, shorten entire breeding cycle.
In addition to size, appearance difference is little for middle seedling and seedlings plant type, primarily directed to flower character carry out breeding, 3.5 cun
Cup flower forcing is to ensure that flower is big, and the present invention is bloomed in 2.5 cun of cup flower forcing, selects pattern, inflorescence with objective trait, can
To reduce the time of or so at least five moon.
Seedling is tested at present also using seedling, 3.5 cun of seedlings flower forcing in 1.5 cun of seedlings, 2.5 cun by the way of, the present invention is using 1.7
Very little seedling is planted, and if seed plants simultaneously flower forcing in 2.8 cun, both can preferably be shown flower character, can also be reduced 5 months
The time of left and right, substantially reduce breeding process.
Description of the drawings
Fig. 1 is the front view of the iris pollen collecting box of the present invention；
Fig. 2 is the vertical view of the iris pollen collecting box box body of the present invention；
In Fig. 1-2：1, box cover, 2, box body, 3, handle, 4 buckles；5, groove, 6, label bag；
Fig. 3 is that iris takes pollen photo figure；As can be seen from the figure：Include anther cap, pollen load handle and glutinous when taking pollen
Disk；
Fig. 4 is to take pollen to stick the figure on paper iris, as can be seen from the figure：Glutinous disk has very strong viscosity, can
It is sticked on paper.
Specific implementation mode
As shown in Figs. 1-2.The iris pollen collecting box of the present invention, including box cover 1, box body 2 and handle 3, the box body
There are two layers, two layers of box body 2 is stacked together, and box cover 1 covers on top layer box body, is similar to the stacked system of food steamer, and box
Lid can seal top layer box body, and top layer box body can seal bottom box body.It is provided on two sides of box cover 1 and bottom box body
Buckle is linked together box cover and bottom box body by buckling, and top layer box body is clipped between bottom box body and box cover.Each box
It is equipped with ten grooves 5 in vivo and is provided with the label bag 6 for placing label for placing iris pollen, the side of groove.
Preferably, the length of the buckle can be adjusted, in order to adjust the number of plies of box body.
Preferably, the box body is per floor height 2cm；Groove size is 3cm × 3cm, deep 0.5cm.
In use, by iris anther cap, pollen load handle and stick to the pan all remove, be sticked to bottom box body using the viscosity sticked to the pan
Groove in, and label is filled in the label bag above groove, after bottom box body use, after being covered box cover, etc.
Preservation.Top layer box body is taken to be further continued for that pollen is taken to be inserted between box cover and bottom box body after top layer box body use,
It is fixed with buckle, classification preservation is carried out to the pollen in different collection boxes according to without usage time.
Following embodiments uses this iris pollen collecting box.
Embodiment 1：
1, pollen is acquired
Iris pollen grain is rolled into a ball by pollen handle and glutinous disk constitutes an entirety, and sticking disk has very strong viscosity, can moment
It is sticked on object.Acquire pollen when, by anther cap, pollen load handle and stick to the pan all remove, be sticked to collection box using the viscosity sticked to the pan
Groove in, and label is filled in the label bag above groove, classification preserves.
2, pollen store method and vitality test
If pollen is using in 1-7 days, 25 DEG C can be no more than in the room temperature highest temperature, when lowest temperature is not less than 18 DEG C
It is kept in greenhouse.
If pollen uses in -2 months 8 days, pollen collecting box must be placed in 4 DEG C of refrigerator and be preserved.
If pollen needs the long period to preserve, it must be used after 2 months, pollen collecting box must be stored in -20 DEG C
Freezing chamber in.Box is encased with valve bag when freezen protective, and is vacuumized as possible, polybag is made to be tightly attached to pollen collecting box
On.Need in use, need to take out in advance, the naturally to thaw under 20 DEG C of environment, when pollen thaw and it is consistent with ambient temperature when, i.e.,
It can be used.Being measured in the pollen activity for preserving 7,14,21,30,45,60 and 180 days for different store methods is as shown in table 1.
The pollen activity of the different store methods of table 1 measures
As can be seen from Table 1：Preserve 2 months at being preserved 21 days, 4 DEG C under room temperature, -20 DEG C preserve 2 months or more, iris
Pollen has preferable activity.
Embodiment 2：Crossbreeding
1, hybridize
Takes powder to be placed in collection box in advance the male parent before iris hybridization, then go directly to plot where maternal plant into
Row hybridization need not be run in the guide between male parent plot and maternal plot, to improve efficiency.
After the pollination for completing paternal pollen, the pollen of maternal plant is collected using collection box for flower as hybridization maternal plant
Get up, make a record preservation, providing pollen as male parent when doing other hybridization uses, and has prevented directly to throw away maternal pollen load
Caused waste；
For the kind of part flowering asynchronism, pollen can be preserved, hybridization is remake when the blossom needed；Glutinous disk
Viscosity can guarantee that it is still firmly sticked on fixture in room temperature in 60 days.
2, seed culture
1) time label is carried out after the completion of hybridization, environment temperature is at 25-30 DEG C, and humidity is in 60-80% conditions normal development 4
A month, you can harvesting fruit pod carries out aseptic seeding.
2) when harvesting fruit pod in greenhouse, fruit pod first is wiped with 75% alcohol swab, is put into closed box after cutting fruit pod
It is temporary.The fruit pod of harvesting with washing powder solution impregnate 20 minutes, after with flowing water rinse 20 minutes, it is spare；
3) iris seed is sterilized 30s on superclean bench with 75% alcohol, afterwards with aseptic water washing 1 time；With 5%
Liquor natrii hypochloritis sterilizes 10min, and constantly rocks, with aseptic water washing 3 times；
Sowing culture medium is：MS+ activated carbon 2.0g/l+ sucrose 25g/l+ agar 6g/l, pH value 5.3-5.5；
Protocorm base is：MS+ peptone 2.0g/l+ banana 100g/l+ activated carbon 1.5g/l+ sucrose 25g/l+ agar
6g/l, pH value 5.3-5.5；
Seedling culture medium is：MS+ activated carbon 2.0g/l+ banana 100g/l+ sucrose 25g/l+ agar 6g/l, pH value 5.3-
5.5；
Environment temperature is at 25-28 DEG C, after planting dark processing, and it is small to be changed to illumination+8 in 16 hours when protocorm starts growth
When it is dark.
2, seedling seedling is planted
Aseptic seedling is tamed after being moved out from laboratory in greenhouse 1 month, is later planted seedling transparent in 1.5 cun
In nutritive cube, illumination controls after 5000-7000lux, one month, clear water, rich water alternate irrigation, and rich water is using 3000 times of dilutions
20-20-20 nitrogen-phosphorus-potassium compound fertilizer, while being stepped up intensity of illumination, control within 10000lux.Greenhouse temperature
Control keeps ventilation at 25-30 DEG C.
3, seed is planted in seedling
After seedling is planted 4 months, basin is changed to 2.5 cun of transparent nutritive cubes, clear water of rich water alternating, rich water use twice
The nitrogen-phosphorus-potassium compound fertilizer of 2000 times of diluted 20-20-20.
4, seedling flower forcing in seedling
After middle seed is planted 4 months, flower forcing processing is proceeded by, 1-2 months before flower forcing processing, improves phosphate fertilizer concentration, fertilising
When with the nitrogen-phosphorus-potassium compound fertilizer of 1500-2000 times of 10-30-20 (rich water, clear water alternately, an i.e. rich water, primary clear
Hereafter water pours clear water every time until the 1st is spent in full bloom)；Flower forcing mode is：25-28 DEG C of day temperature, night temperatures 18-
20 DEG C, intensity control is bloomed, is selected according to flower fancy points suitable in 20000-25000lux, flower forcing for 120 days or so successively
Kind.
For big gaily-colored butterfly orchid species, flower shape that middle seedling is bloomed, pattern are consistent with 3.5 cun of seedlings, the flower of middle seedling
Piece diameter, flower number are slightly below 3.5 cun of cups.Under normal conditions, than 3.5 cun seedlings of flower of seedling are small in middle flower pattern iris
1.0cm, flower number are 2 or so few；The flower diameter of seedling and seedlings are suitable in little Hua multi-pattern type irises, and flower number is less slightly, root
Depending on offspring's feature of Different Cross Combinations.
5, the mitogenetic seedling test of outstanding single plant
It is excellent for the trait expression of blooming of seedling in 2.5 cun, it blooms without 3.5 cun of seedlings, detection plant virus confirms nothing
Viral plant directly can carry out sterile mitogenetic fast numerous, the test group of 300 plants or so of formation by cut-flower stalk.Seedling field planting to be tested
To after greenhouse, carry out starting flower forcing after seed is planted 4 months in seedling plantation 4 months, 2.8 cun of cups through 1.7 cun of cups, Post flowering carries out
Consistency, stability, the specific test of new varieties.The kind of test passes can apply for that kind is protected, and carry out marketing.
The present invention is had found by breeding practice for many years：When 2.5 cun of cups of iris, as long as giving above-mentioned specific environment item
Part can equally output flower, observe filial generation phenotype in 2.5 cun of cups (middle seedling stage) flower forcing, can shorten the seed selection time, together
When, the outstanding single plant selected can enter sterile expansion numerous stage in advance, shorten entire breeding cycle.
The present invention is also had found by breeding practice：Pair 2.5 cun of cup irises carry out flower forcing, table on the basis of embodiment 2
Existing excellent middle seedling cuts off bennet, changes 3.5 cun of cups and continues plantation and wait for reflorescence, rejects the seedling of poor performance；To 3.5
Very little cup iris detection plant virus, confirm virus-free plant can directly cut-flower stalk carry out it is sterile mitogenetic fast numerous.This mode with
The present invention is bloomed without 3.5 cun of seedlings, and it is sterile mitogenetic fast numerous that 2.5 cun of cups detect the direct cut-flower stalk progress of virus-free plant.On
It is little to state two ways its final result difference, continues plantation and waits for reflorescence changing 3.5 cun of cups, reject the reality of poor performance
Raw seedling, the rejecting rate of seedling have substantially no effect on final result less than 1%.
Claims (10)
1. a kind of iris pollen store method, characterized in that when iris pollen collection, by anther cap, pollen load handle and glutinous disk
It all removes, is sticked in pollen collecting box using the viscosity of glutinous disk, and preserved after marking.
2. a kind of iris pollen store method as described in claim 1, characterized in that
If pollen uses in 1-7 days, kept at 18-25 DEG C；
If pollen uses in -2 months 8 days, pollen collecting box is placed in 4 DEG C of refrigerator and is preserved；
If pollen uses after 2 months, pollen collecting box is stored in -20 DEG C of freezing chamber.
3. a kind of iris pollen store method as claimed in claim 2, characterized in that preserve butterfly in -20 DEG C of freezing chambers
Orchid powder encases box with valve bag when freezen protective, vacuumizes, polybag is made to be tightly attached on pollen collecting box；It will when use
Pollen collecting box naturally to thaw under 20 DEG C of environment, when pollen thaw and it is consistent with ambient temperature when use.
4. the matching used iris pollen collecting box of store method described in a kind of any one of and claim 1-3, packet
Box body and box cover are included, box cover covers on box body, characterized in that at least 1 layer of the box body, and each layer box body is stacked together；
It is equipped with multiple grooves in every layer of box body and is provided with the label bag for placing label for placing iris pollen, the side of groove.
5. a kind of iris pollen store method as claimed in claim 4, characterized in that be equipped with 10- in every layer of box body
20 grooves.
6. iris pollen collecting box as claimed in claim 4, characterized in that the high 2cm of every layer of box body；Groove size is 3cm
× 3cm, deep 0.5cm.
7. iris pollen collecting box as claimed in claim 4, characterized in that 2 sides of the box cover and bottom box body
Or it is provided with buckle on 4 sides, box cover and bottom box body are linked together by buckling, intermediate box body is clipped in bottom box
Between body and box cover.
8. a kind of iris breeding method, characterized in that
1) iris hybridizes
Iris pollen is preserved using the iris pollen store method described in any one of claim 1-3；
Takes powder to be placed in pollen collecting box in advance the male parent before iris hybridization, then go directly to plot where maternal plant into
Row hybridization；
The flower of maternal plant is collected using pollen collecting box after the pollination for completing paternal pollen, makes a record preservation,
Pollen is provided when doing other hybridization as male parent to use；
For the kind of part flowering asynchronism, pollen is preserved using pollen collecting box, is remake when the blossom needed miscellaneous
It hands over；
2) breeding
It harvests hybrid seed and carries out aseptic seeding, flower forcing processing is carried out when growing to 2.5 cun of cups of middle seedling, according to flower fancy points
Select suitable kind；
It is excellent for the trait expression of blooming of seedling in 2.5 cun of cups, it blooms without 3.5 cun of seedlings, it is direct to detect virus-free plant
Cut-flower stalk carries out sterile mitogenetic fast numerous, the test group of 300 ± 20 plants of formation；
After seedling field planting to greenhouse to be tested, carries out seedling through 1.7 cun of cups and plant 4 months, after seed is planted 4 months in 2.8 cun of cups
Start flower forcing, Post flowering carries out consistency, stability, the specific test of new varieties.
9. a kind of iris breeding method as claimed in claim 8, characterized in that the flower forcing mode is：Day temperature
25-28 DEG C, 18-20 DEG C of night temperatures, intensity control is in 20000-25000lux.
10. a kind of iris breeding method as claimed in claim 8, characterized in that 1-2 months before the flower forcing processing, fertilizer
Water, clear water alternately, are spent in full bloom up to the 1st；The rich water is：N：P2O5：K2O=10：30：20 N P and K is compound
1500-2000 times of dilution of fertilizer.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113465298A (en) * | 2021-07-16 | 2021-10-01 | 四川农业大学 | South Asia loquat pollen drying and storing method |
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