CN108651162A - A kind of preparation method and products thereof of Antialcoholic liver-protecting Agricus blazei zymotic fluid - Google Patents
A kind of preparation method and products thereof of Antialcoholic liver-protecting Agricus blazei zymotic fluid Download PDFInfo
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- CN108651162A CN108651162A CN201810377138.1A CN201810377138A CN108651162A CN 108651162 A CN108651162 A CN 108651162A CN 201810377138 A CN201810377138 A CN 201810377138A CN 108651162 A CN108651162 A CN 108651162A
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Environmental Sciences (AREA)
- Microbiology (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention provides the preparation methods of a kind of Antialcoholic liver-protecting Agricus blazei and its tunning：Agaricus blazei is cultivated to activation on slant medium first, obtains work strain；Then it is seed liquor to be inoculated in shaker fermentation in seed culture medium；It is finally inoculated in air agitation fermentation in the fermentation medium containing wolfberry fruit extract and aloe extract and obtains the zymotic fluid containing Agricus blazei and its tunning.
Description
Technical field
The invention belongs to field of food industry, and in particular to a kind of preparation method of Antialcoholic liver-protecting Agricus blazei zymotic fluid.
Background technology
China is the native place of white wine, and it is bad that suitable drinking white spirit can promote blood to follow, and improves sleep, eases off the pressure.But
It is that excessive consumption of alcohol inherently causes to bear to liver, because the decomposition of alcohol needs liver to participate in, alcohol makees the damage of liver
With mainly caused by ethyl alcohol and its metabolite acetaldehyde.A large amount of aggregations of ethyl alcohol and acetaldehyde can reduce the biology of each enzyme in liver cell
Activity influences the eubolism of sugar, fat and protein, changes membrane structure, causes liver cell destruction, fat deposition, very
To liver fibrosis, hepatic sclerosis.ALT（Glutamic-pyruvic transaminase）、AST（Glutamic-oxalacetic transaminease）It is primarily present in nucleus and mitochondria,
Blood is released into when liver cell is obviously damaged, the raising of serum alt, AST is to reflect the specific index of hepatic injury.For
Hepatic injury caused by improvement alcohol is badly in need of finding a kind of safely and effectively resource of exploitation.
Agricus blazei（Agaricus blazei Munrill）Also known as Brazilian mushroom, Bai Shi mushrooms, little Song mushrooms, belong to load
Bacterium subphylum, Hymenomycetes, Agaricales, Agaricus edibilis, Agaricus are a kind of rare fungies of food and medicament dual-purpose.Its source area is distributed in north
The ground such as U.S. south and South America the north, 1992, China introduced Agaricus blazei, and is studied it.Agricus blazei quality
It is tender and crisp, it is in good taste, there is multiple nutritional components such as carbohydrate, protein, fiber, fat and abundant vitamin and mineral.
Recent study is found, multiple biological activities ingredient such as polysaccharide, sterol, agglutinin, nucleic acid etc. is contained in Agricus blazei fructification, it
Can antitumor, reducing blood lipid, blood pressure lowering, strengthen immunity safeguards liver function, treats diabetes.In terms of hepatoprotective effect, have
Research confirms that Agricus blazei has the function of significantly inhibiting liver cancer cells, can also improve the clinical symptoms of hepatitis B patient,
There is protective effect to hepar damnification.The culture of Agricus blazei mainly has solid state cultivation and liquid deep layer fermenting culture two ways,
But since the solid state cultivation production cycle is long, easily affected by environment, yield is relatively low, it is difficult to meet ever-increasing market demand,
And the liquid deep layer fermenting time is short, influence condition is controllable, is a kind of training method with bright prospects.Agricus blazei passes through liquid
After fermentation, zymotic fluid, mycelium and fructification nutrition having the same and active constituent and health-care effect.In addition, there is research
Show that aloe extract and wolfberry fruit extract can improve superoxide dismutase（SOD）Activity, to alcoholic liver injury
There is certain protective effect.
Invention content
The problems such as the food and medicine for lacking hepatic injury caused by improving alcohol, the present invention provides one kind and is carried with containing aloe
The preparation method of the zymotic fluid of object and the Agricus blazei of wolfberry fruit extract fermentation is taken, the product effects of relieving alcoholism and protecting liver of preparation is good, is better than
The effect of each separate constituent.
To achieve the above object, the present invention adopts the following technical scheme that.
The preparation method of a kind of Antialcoholic liver-protecting Agricus blazei and its tunning, includes the following steps：
（1）Agaricus blazei cultivates activation on slant medium, obtains work strain；
（2）It is seed liquor that work strain, which is inoculated in shaker fermentation in seed culture medium,；
（3）Seed liquor is inoculated in air agitation fermentation in the fermentation medium containing wolfberry fruit extract and aloe extract mixture and obtains
The zymotic fluid of Agricus blazei and its tunning must be contained.
The seed culture medium is prepared using following methods：
A) 20% peeled potatoes are weighed, stripping and slicing adds boiling rotten, and filtered through gauze obtains potato juice；
B) it weighs after boiling 20min in 1% wheat bran addition potato juice, gauze filters to get filtrate again；
C) filtered juice is added in the glucose for weighing 2% while hot, and 0.1% KH is added after dissolving2PO4With 0.1% MgSO4；
The % is the weight percentage of seed culture medium.
The fermentation medium is prepared using following methods：
A) 20% peeled potatoes are weighed, stripping and slicing adds boiling rotten, and filtered through gauze obtains potato juice；
B) it weighs after boiling 20min in 1% wheat bran addition potato juice, gauze filters to get filtrate again；
C) filtered juice is added in the glucose for weighing 2% while hot, and 0.1% KH is added after dissolving2PO4With 0.1% MgSO4；
D) it weighs and is stirred evenly in mixture addition c) of 1-10% aloe extracts and wolfberry fruit extract；
The % is the weight percentage of fermentation medium.
The content of the aloe extract and wolfberry fruit extract is preferably 3-7%；The aloe extract and wolfberry fruit extract
Mixture in the mass ratio of aloe extract and wolfberry fruit extract be 1:1-1:10.
Preferably, the step（2）Middle seed liquor can be obtained through multistage fermentation.
Preferably, the step（3）Further include the steps that by separation of fermentative broth mycelium obtain clear liquid, preferably centrifugation or
Person filters；More preferably filter.It is furthermore preferred that the step（3）It further include the step that separation of fermentative broth mycelium is obtained to clear liquid
Rapid and drying steps.
Step（1）-（3）In, culture or fermentation temperature are 23-28 DEG C, preferably 25-28 DEG C.Incubation time is 4-7 days.
Step（2）-（3）In, rotating speed 150-200rpm.Step（3）In, ventilatory capacity 2-5vvm.
The content of wolfberry fruit extract is preferably 20-50% wt in the wolfberry fruit extract.
Preferably, the wolfberry fruit extract is prepared using following methods：The matrimony vine cleaned is taken, 8-10 times is added and measures water, is boiled
It boils 1-2 hours, filters to obtain a decoction liquor；Filter residue adds 5-8 times to measure water, decocts 40-60min, filters to obtain secondary decoction liquor, merges
Decoction liquor twice, be concentrated into relative density be 1.10-1.20 when, be spray-dried, obtain dry powder, i.e. wolfberry fruit extract.
The content of barbaloin is preferably 15-20% wt in the aloe extract.
Preferably, the aloe extract is prepared using following methods：Aloe barbadensis Miller powder is placed in 10-20 volumes times
40-60%（v/v）Ethyl alcohol（pH=5）In, it is extracted 2 times, each 45-60min with 300-400W power ultrasonics, Extracting temperature is
60-100 DEG C, merges concentration, drying after extracting solution twice, obtain dry powder, i.e. aloe extract.The pH is using dilute hydrochloric acid, dilute sulphur
Acid or acetic acid are adjusted.The method of the concentration is preferably evaporated under reduced pressure.The drying is preferably spray-dried or freeze-drying.
A kind of Agricus blazei and its tunning that the above method obtains.The invention also includes contain above-mentioned Agricus blazei, Ji Song
The functional food with effects of relieving alcoholism and protecting liver of fine and soft tunning or Agricus blazei and its tunning.The functional food
Dosage form is preferably tablet, pulvis, granule, capsule and oral solution.
The present invention has the following advantages：
The present invention cultivates Agricus blazei using liquid deep layer fermenting mode, is suitable for industrialized production；The culture solution of the present invention
Main component, if potato, wheat bran are all the agricultural product being easy to get at a low price, zymotic fluid production cost is low.The present invention is in Agricus blazei liquid
Aloe extract and wolfberry fruit extract are added in body fermentation medium, the water of ALT and AST in mice serum can be significantly reduced
It is flat, the liver-protecting efficacy of Agricus blazei zymotic fluid is improved, has the function that synergy.
Specific implementation mode
With reference to embodiment, the present invention will be further described, but the present invention is not limited by following embodiments.
The preparation of 1 Agricus blazei zymotic fluid of embodiment
（1）The preparation of wolfberry fruit extract：
The matrimony vine cleaned is taken, 10 times of amount water are added, boiling is boiled 1.5 hours, and a decoction liquor is filtered to obtain；Filter residue adds 8 times of amount water, decocts
45min, filters to obtain secondary decoction liquor, merges decoction liquor twice, when to be concentrated into relative density be 1.17, be spray-dried, obtained
Dry powder, i.e. wolfberry fruit extract；Its polysaccharides content is 23.8%.
（2）The preparation of aloe extract：
By ethyl alcohol with vinegar acid for adjusting pH=5, and volume fraction is 50%；Then aloe barbadensis Miller powder is placed in 10 volumes above-mentioned second again
In alcohol, extracted 2 times, each 60min with 300W power ultrasonics, Extracting temperature is 90 DEG C, merge vacuum distillation after extracting solution twice,
Spray drying, obtains dry powder, i.e. aloe extract；Its barbaloin content is 17.36%.
（3）Prepare the culture medium used in fermentation process：
The inclined-planes PDA：The potato that 200g has been removed the peel is weighed, is cut into small pieces, adds 1000mL boilings rotten（Fritter can be rolled into mud）,
Potato juice, moisturizing to 1000mL are obtained with eight layers of filtered through gauze；20g glucose is added into potato juice in a heated state,
After stirring evenly, the agar powder of 20g is added, until completely dissolved, is sub-packed in test tube, adds tampon, it is tight that outer layer wraps up in paper bag, and 121
Under DEG C 100kPa after high-temperature sterilization 20min, test tube is taken out, is put into inclined-plane, it is spare after cooling；
Seed liquid culture medium：The potato that 200g has been removed the peel is weighed, is cut into small pieces, adds 1000mL boilings rotten（Fritter can be rolled
At mud）, potato juice is obtained with eight layers of filtered through gauze, 10g wheat brans are added into potato juice in a heated state boils 20min
Afterwards, with 16 layers of filtered through gauze, moisturizing to 1000mL；20g glucose is added toward filtered juice in a heated state, after stirring evenly,
1g KH are added2PO4With 1g MgSO4, pH adds cotton naturally, be sub-packed in the 250mL triangular flasks containing 150mL seed liquid culture mediums
Plug, it is tight that outer layer wraps up in paper bag, is taken out after high-temperature sterilization 20min under 121 DEG C of 100kPa, spare after cooling；
Liquid fermentation medium：The potato that 200g has been removed the peel is weighed, is cut into small pieces, adds 1000mL boilings rotten（Fritter can be ground
It is pressed into mud）, potato juice is obtained with eight layers of filtered through gauze, 20g wheat brans are added into potato juice in a heated state boils 20min
Afterwards, with 16 layers of filtered through gauze, the glucose of 20g is added toward filtered juice in a heated state, after stirring evenly, 1g KH are added2PO4
With 1g MgSO4, the mixture of 60g aloe extracts and wolfberry fruit extract（Aloe extract：Wolfberry fruit extract=1:5）, pH is certainly
So, it is sub-packed in the 500mL triangular flasks containing 300mL liquid fermentation mediums, adds tampon, it is tight that outer layer wraps up in paper bag, 121 DEG C
It is taken out after high-temperature sterilization 20min under 100kPa, it is spare after cooling.
（4）Agricus blazei zymotic fluid is prepared according to the methods below：
Actication of culture：The mycelia for hooking the Agricus blazei mother's strain for taking 1 piece of soya bean size with transfer needle in superclean bench, is inoculated in
In PDA culture medium, it is spare after 4 days to be put into 28 DEG C of activation cultures in constant incubator for sealing；
Inoculation：By 4-5 block diameters 1cm2Above-mentioned pure culture biscuits involvng inoculation in seed liquid culture medium, sealing, be put into constant incubator
In 28 DEG C of shaking table 180r/min cultivate 5 days；
Liquid fermentation and culture：Seed liquor is inoculated in by inoculum concentration 10% in liquid fermentation medium, with 180r/min at 28 DEG C
Mixing speed and 2vvm air agitations culture 6 days obtain zymotic fluid.
The preparation of 2 Agricus blazei zymotic fluid of embodiment
（1）The preparation process of the culture medium used in fermentation process such as embodiment 1, difference are aloe in liquid fermentation medium
The mixture additive amount of extract and wolfberry fruit extract is 70g, wherein aloe extract：Wolfberry fruit extract=1:6；
（2）Agricus blazei zymotic fluid is prepared according to the methods below：
Actication of culture：The mycelia for hooking the Agricus blazei mother's strain for taking 1 piece of soya bean size with transfer needle in superclean bench, is inoculated in
In PDA culture medium, it is spare after 7 days to be put into 25 DEG C of activation cultures in constant incubator for sealing；
Inoculation：By 4-5 block diameters 1cm2Above-mentioned pure culture biscuits involvng inoculation in seed liquid culture medium, sealing, be put into constant incubator
In 25 DEG C of shaking table 150r/min cultivate 7 days；
Liquid fermentation and culture：Seed liquor is inoculated in by inoculum concentration 10% in liquid fermentation medium, with 150r/min at 25 DEG C
Mixing speed and 3vvm air agitations culture 7 days obtain zymotic fluid；
Filtering：Above-mentioned zymotic fluid is obtained into Agricus blazei zymotic fluid with 0.22 μm of membrane filtration.
The preparation of 1 Agricus blazei zymotic fluid of comparative example
（1）The preparation process of the culture medium used in fermentation process such as embodiment 1, difference are not add in liquid fermentation medium
Add the mixture of aloe extract and wolfberry fruit extract；
（2）The preparation method of Agricus blazei zymotic fluid is as described in Example 1, obtains zymotic fluid.
The preparation of 2 Agricus blazei zymotic fluid of comparative example
（1）The preparation process of the culture medium used in fermentation process such as embodiment 2, difference are not add in liquid fermentation medium
Add the mixture of aloe extract and wolfberry fruit extract；
（2）The preparation method of Agricus blazei zymotic fluid is as described in Example 2, obtains zymotic fluid.
Effect of the 3 Agricus blazei zymotic fluid of embodiment to alcoholic hepatic injury
It is prepared by 3.1 Agricus blazei fermentation broth samples
The Agricus blazei zymotic fluid for completion of fermenting in embodiment 1 and comparative example 1 is poured into tissue refiner after being pounded homogenate and is poured out
It is placed in container, high fire heating 5min, seals, after cooling, be put into 4 DEG C of refrigerator and save backup, fermented in micro-wave oven
Liquid 1（Embodiment 1）With zymotic fluid CK1（Comparative example 1）；The mixed of 6% aloe extract and wolfberry fruit extract is added in zymotic fluid CK1
Close object（Aloe extract：Wolfberry fruit extract=1:5）Zymotic fluid CK2 is obtained after homogeneous.
3.2 alcohol induced liver injury mouse modelings and experiment process
Mouse adaptable fed 7 days is randomly divided into blank group, model group, control group 1, control group 2, fermentation group, totally three groups, often
Group 10, it is 3 weeks to establish the model period, during which supplies normal diet, and dietary restriction is not drunk water.It first week, pinpoints give daily
56 degree of model group, control group 1, control group 2, sample sets white wine gavages, given low 0.08mL/10gbw, blank group are given
The distilled water of same dose, after 4 hours, 1 sample of zymotic fluid in fermentation group gavage 3.1, dosage is 0.4mL/, and control group 1 is given
Zymotic fluid CK1 samples are given, dosage 0.4mL/, control group 2 give zymotic fluid CK2 samples, dosage 0.4mL/, blank group and mould
Type group gives the distilled water of same dose.2nd week, the given low of white wine increased to 0.10mL/10gbw, remaining operation is the same as the
One week.3rd week, the given low of white wine increased to 0.12mL/10gbw, remaining operation was the same as first week.Test last 1 day gavage
After, three groups of mouse fasting are for 24 hours.Outside the dead mouse of removal, every group takes 7 eyeball of mouse to take blood, is placed in and heparin sodium has been added
EP pipes in, and quickly dislocation put to death.Blood is subjected to ALT, AST detection.
The influence of 1 Agricus blazei of table and its zymotic fluid to ALT, AST level in alcoholic hepatic injury mice serum
| Group | ALT(U/L) | AST(U/L) |
| Blank group | 48.65±3.31a | 147.31±9.89a |
| Model group | 80.17±5.34b | 197.36±12.92b |
| Fermentation group | 48.87±3.65a | 146.87±11.67a |
| Contrast groups 1 | 65.64±4.24c | 179.34±16.22c |
| Contrast groups 2 | 63.96±4.03c | 172.45±12.55c |
Note：Different letters indicate p when LSD is examined<0.05.
3.3 prepared by Agricus blazei fermentation broth sample
By the Agricus blazei zymotic fluid for completion of fermenting in embodiment 2 and comparative example 2, high fire heating 5min, sealing wait in micro-wave oven
After cooling, it is put into 4 DEG C of refrigerator and saves backup, obtain zymotic fluid 1 '（Embodiment 2）With zymotic fluid CK1 '（Comparative example 2）；It will fermentation
The mixture of 7% aloe extract and wolfberry fruit extract is added in liquid CK1 '（Aloe extract：Wolfberry fruit extract=1:6）After homogeneous 1h
It crosses 0.22 μm of filter membrane and obtains zymotic fluid CK2 '.
3.5 alcohol induced liver injury mouse modelings and experiment process
Mouse adaptable fed 7 days is randomly divided into blank group, model group, control group 1, control group 2, fermentation group, totally three groups, often
Group 10, it is 3 weeks to establish the model period, during which supplies normal diet, and dietary restriction is not drunk water.It first week, pinpoints give daily
56 degree of model group, control group 1, control group 2, sample sets white wine gavages, given low 0.08mL/10gbw, blank group are given
The distilled water of same dose, after 4 hours, 1 ' sample of zymotic fluid in fermentation group gavage 3.4, dosage is 0.4mL/, and control group 1 is given
Give zymotic fluid CK1 ' samples, dosage 0.4mL/ only, control group 2 give zymotic fluid CK2 ' samples, dosage 0.4mL/ only, blank group and
Model group gives the distilled water of same dose.2nd week, the given low of white wine increased to 0.10mL/10gbw, remaining operation is same
First week.3rd week, the given low of white wine increased to 0.12mL/10gbw, remaining operation was the same as first week.It tests last 1 day and fills
After stomach, three groups of mouse fasting are for 24 hours.Outside the dead mouse of removal, every group takes 7 eyeball of mouse to take blood, is placed in and heparin has been added
In the EP pipes of sodium, and quickly dislocation is put to death.Blood is subjected to ALT, AST detection.
Influence of the 2 Agricus blazei zymotic fluid of table to ALT, AST level in alcoholic hepatic injury mice serum
| Group | ALT(U/L) | AST(U/L) |
| Blank group | 45.54±3.49a | 143.18±10.20a |
| Model group | 78.85±5.36b | 194.32±13.62b |
| Fermentation group | 52.91±4.53a | 152.60±12.73a |
| Contrast groups 1 | 68.42±4.03d | 183.19±14.15c |
| Contrast groups 2 | 67.14±3.71d | 180.23±10.65c |
Note：Different letters indicate p when LSD is examined<0.05.
From the data of table 1, table 2：It is compared with blank group, ALT, AST level obviously increase in model group mice serum.
It is compared with model group, ALT, AST level decrease in the mice serum of contrast groups 1 and contrast groups 2.It is compared with model group, hair
ALT, AST level are substantially reduced in ferment group mice serum, and little with the horizontal difference of blank group.Compared with contrast groups 1 or 2,
ALT, AST level are substantially reduced in fermentation group mice serum.It is extracted with matrimony vine it follows that adding aloe extract in the present invention
The Agricus blazei and its tunning of object or the tunning of Agricus blazei, which can be reduced, to be caused by alcohol in hepatic injury mice serum
ALT, AST are horizontal, and aloe extract leads to alcohol with Agricus blazei co-fermentation with wolfberry fruit extract the protection of hepatic injury
Effect is more preferable.
4 Agricus blazei of embodiment and its tunning tablet
Spray drying after the zymotic fluid obtained in embodiment 1 vacuum distillation concentration is obtained into dry powder；100g dry powder and 200g are formed sediment
Tabletting after powder, 50g sucrose, the mixing of 150g hydroxymethyl celluloses, is made the tablet of 1g/ pieces.
5 Agricus blazei of embodiment and its tunning granule
Spray drying after the zymotic fluid obtained in embodiment 1 vacuum distillation concentration is obtained into dry powder；It is beautiful that 50g is added per 100g dry powder
Rice starch, 50g dextrin, 0.25g Sucraloses and the mixing of 500g water, extruder grain, Sieving and casing is 3-5mm after drying, is obtained
Granule；Unqualified particulate material adds the granulation that feeds intake again after water.
6 Agricus blazei tunning oral solution of embodiment
10g citric acids, 0.5g steviol glycosides, 5g xylitols, homogenizer is added according to every liter in the zymotic fluid obtained in embodiment 1
The filling oral solution for 10mL/ branch after the homogeneous 20min of mesohigh.
Claims (10)
1. the preparation method of a kind of Antialcoholic liver-protecting Agricus blazei and its tunning, which is characterized in that include the following steps：
（1）Agaricus blazei cultivates activation on slant medium, obtains work strain；
（2）It is seed liquor that work strain, which is inoculated in shaker fermentation in seed culture medium,；
（3）Seed liquor is inoculated in air agitation fermentation in the fermentation medium containing wolfberry fruit extract and aloe extract mixture and obtains
The zymotic fluid of Agricus blazei and its tunning must be contained.
2. preparation method according to claim 1, which is characterized in that the mixing of the aloe extract and wolfberry fruit extract
Object addition is the 1-10% wt of culture solution；Aloe extract and Chinese holly in the mixture of the aloe extract and wolfberry fruit extract
The mass ratio of Qi extract is 1:1-1:10.
3. preparation method according to claim 1 or 2, which is characterized in that the aloe extract and wolfberry fruit extract
Addition is 3-7% wt.
4. preparation method according to claim 1, which is characterized in that the step（2）Middle seed liquor can ferment through multistage
It obtains.
5. preparation method according to claim 1, which is characterized in that the step（3）Further include by separation of fermentative broth bacterium
Filament obtains the step of clear liquid；Preferably, the step（3）Further include the steps that by separation of fermentative broth mycelium obtain clear liquid and
Drying steps.
6. preparation method according to claim 1, which is characterized in that step（1）-（3）In, culture or fermentation temperature are
23-28 DEG C, preferably 25-28 DEG C；Incubation time is 4-7 days；Step（2）-（3）In, rotating speed 150-200rpm；Step（3）
In, ventilatory capacity 2-5vvm.
7. preparation method according to claim 1, which is characterized in that the content of wolfberry fruit extract in the wolfberry fruit extract
Preferably 20-50% wt；The content of barbaloin is preferably 15-20% wt in the aloe extract.
8. a kind of Agricus blazei and its tunning obtained according to any preparation methods of claim 1-7.
9. a kind of tool containing Agricus blazei as claimed in claim 8, Agricus blazei tunning or Agricus blazei and its tunning
There is the functional food of effects of relieving alcoholism and protecting liver.
10. functional food according to claim 9, which is characterized in that dosage form be tablet, pulvis, granule, capsule and
Oral solution.
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