CN108651161A - A kind of Chinese caterpillar fungus culture medium and preparation method thereof - Google Patents
A kind of Chinese caterpillar fungus culture medium and preparation method thereof Download PDFInfo
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- CN108651161A CN108651161A CN201810325965.6A CN201810325965A CN108651161A CN 108651161 A CN108651161 A CN 108651161A CN 201810325965 A CN201810325965 A CN 201810325965A CN 108651161 A CN108651161 A CN 108651161A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
Abstract
A kind of Chinese caterpillar fungus culture medium, using water as solvent, by weight, the raw material that the Chinese caterpillar fungus culture medium is made includes following components：30 70 parts of major ingredient, 5 20 parts of lepidopterous insects pupa powder, 3 12 parts of glucose, 28 parts of peptone, 15 parts of cornstarch, 0.5 5 parts of ammonium citrate, 0.5 5 parts of magnesium sulfate, 0.5 5 parts of potassium dihydrogen phosphate, 7 12 parts of hydroxyethyl cellulose, 15 30 parts of Chinese medicine additive, the Chinese medicine additive is made of tribulus terrestris, Chinese aloe leaf and Radix Astragali, total weight based on Chinese medicine additive, the content of tribulus terrestris is 40% 55wt%, the content of Chinese aloe leaf is 20 40wt%, the content of Radix Astragali is 5 25 wt%.The preparation method of above-mentioned Chinese caterpillar fungus culture medium is also disclosed in the present invention, it can effectively inhibit varied bacteria growing according to the finally obtained Chinese caterpillar fungus culture medium of the preparation method, accelerate mycelia molding, enhancing fruiting bodies of cordyceps militaris oxidation resistance, there is extremely wide application prospect.
Description
Technical field
The present invention relates to the cultivation fields of Cordyceps militaris, and in particular to a kind of Chinese caterpillar fungus culture medium and preparation method thereof.
Background technology
Cordyceps militaris is also known as Cordceps militaris, northern Chinese caterpillar Fungus, is generally acknowledged both at home and abroad medicinal fungi, the Cordyceps militaris of cultivation
In most the efficacy component cordycepin of essence content than in wild cordyceps it is taller go out several times, and cordycepic acid, adenosine,
The nutritions content such as Cordyceps sinensis polysaccharide, mannitol is also relatively high.Cordyceps militaris has anticancer, invigorating the lung and the kidney, improves immunity
Effect, medical value are high.Not only effective nutrient composition content is relatively low for the existing culture medium for indoor culture Cordyceps militaris, and
Will appear the interference of miscellaneous bacteria during mycelia grows up to, both sides influence causes the mycelial growth rate of Cordyceps militaris slower,
In addition, being oxidized easily by the fruiting bodies of cordyceps militaris that mycelia is cultivated and being failed, unnecessary waste is caused.
By taking the Chinese patent of 105503375 A of Publication No. as an example, which disclose a kind of efficient Cordyceps militaris spawns to train
Base is supported, the proportioning used is as follows：10~20g/L of peptone, 3~5g/L of yeast powder, 3~5g/L of analysis for soybean powder, corn steep liquor 5~
10g/L, 15~30g/L of glucose, 1.2~1.5g/L of potassium dihydrogen phosphate, 0.8~1.2g/L of sodium chloride, 1~1.2g/ of magnesium sulfate
L, 3~5g/L of biotin, 2% 1~3g/L of yolk.Although the culture medium has been obtained for extensive use, but it can not be solved
State the defect of " varied bacteria growing, mycelia molding is slow, fruiting bodies of cordyceps militaris oxidation resistance is poor " mentioned.
Invention content
The primary purpose of the present invention is that varied bacteria growing can effectively be inhibited, accelerate mycelia molding, enhancing pupa worm by providing one kind
The Chinese caterpillar fungus culture medium of grass seed entity oxidation resistance.
Another object of the present invention is to provide the preparation methods of above-mentioned Chinese caterpillar fungus culture medium.
To solve the above-mentioned problems, using following technical scheme：A kind of Chinese caterpillar fungus culture medium, using water as solvent, by weight
Part meter, the raw material that the Chinese caterpillar fungus culture medium is made includes following components：30-70 parts of major ingredient, 5-20 parts of lepidopterous insects pupa powder, Portugal
3-12 parts of grape sugar, 2-8 parts of peptone, 1-5 parts of cornstarch, 0.5-5 parts of ammonium citrate, 0.5-5 parts of magnesium sulfate, potassium dihydrogen phosphate
0.5-5 parts, 7-12 parts of hydroxyethyl cellulose, 15-30 parts of Chinese medicine additive, the Chinese medicine additive by tribulus terrestris, Chinese aloe leaf and
Radix Astragali forms, and the total weight based on Chinese medicine additive, the content of tribulus terrestris is 40%-55wt%, the content of Chinese aloe leaf is 20-
40wt%, Radix Astragali content be 5-25 wt%.The sum of above-mentioned tribulus terrestris, Chinese aloe leaf, Radix Astragali content are 100wt%.
Preferably, the content of tribulus terrestris is 50wt%, the content of Chinese aloe leaf is 30wt%, the content of Radix Astragali is 20wt%.
It is highly preferred that the mass ratio of hydroxyethyl cellulose and Chinese medicine additive is 1：2.
Wherein, one or several kinds of mixtures of the above-mentioned major ingredient in rice, wheat, corn or soya bean.
Wherein, above-mentioned lepidopterous insects pupa powder is dried silkworm chrysalis meal.
Wherein, the parts by weight of above-mentioned water are 50-100.
A kind of Chinese caterpillar fungus culture medium further includes 0.5-5 parts of vitamin, and said vitamin is by vitamin B1, vitamin B2, dimension
Raw element B6And vitamin B12Composition.
The method for preparing Chinese caterpillar fungus culture medium, includes the following steps：
（1）Glucose, peptone, cornstarch, ammonium citrate, magnesium sulfate, potassium dihydrogen phosphate are added to the water, battalion is stirred to get
Nutrient solution is spare；
（2）It is added to the water after tribulus terrestris, Chinese aloe leaf and Radix Astragali are mixed, then hydroxyl second is added in boiling 4-6h while stirring
Base cellulose continues to filter after heating 1-1.5h, obtains filtrate and the dregs of a decoction are spare controlled at 50-60 DEG C；
（3）By major ingredient grinds, it then is sequentially added into nutrient solution with lepidopterous insects pupa powder, filtrate, the dregs of a decoction, is stirred evenly,
Obtain mixed liquor；
（4）Mixed liquor is dispensed into culture vessel, and 30min-60min is sterilized under 115 DEG C of -140 DEG C of high temperature, is cooled to 25
DEG C up to Chinese caterpillar fungus culture medium.
Tribulus terrestris is containing abundant tribulus terrestris saponin, Flavonoid substances, fat oil, nitrate.Tribulus terrestris saponin has anti-grease
Matter oxidation can remove hydroxyl radical free radical and prevent Cordyceps militaris from being aoxidized；Fat oil is readily flowed, convenient for being inhaled by Cordyceps militaris
It receives and utilizes；And nitrate can be provided as culture medium and provide nitrogen source to promote the growth of Cordyceps militaris；Flavonoid substances are to grape ball
Bacterium, streptococcus, Diplococcus pneumopniae, bacillus anthracis, Escherichia coli, shigella dysenteriae, typhoid bacillus, proteus etc. have inhibition to make
With.
Carbohydrate, protein content in Chinese aloe leaf is higher, and the content of protein is close to 10%, these protein are after hydrolysis
19 kinds of amino acid, including arginine, isoleucine, leucine, phenylalanine, lysine, threonine, tryptophan and figured silk fabrics can be generated
Propylhomoserin, in addition, Chinese aloe leaf is also micro containing abundant vitamin and potassium, sodium, calcium, magnesium, aluminium, iron, silicon, zinc, copper, barium etc.
Element.Chinese aloe leaf also contains cuspidatin, has the function of inhibiting miscellaneous bacteria, have especially for staphylococcus, streptococcus
Extremely strong inhibiting effect.
Astragalus polyose in Radix Astragali can effectively improve the immunity of Cordyceps militaris, reduce the probability of its illness, in Radix Astragali
Astragaloside, astragalus polyose, Flavonoid substances have remove free radical the effect of, can anti-lipid peroxidation damage, remove
Oxygen radical prevents Cordyceps militaris from shortening the service life because of oxidation.
Compared to the prior art, the invention has the advantages that：
The present invention is removed to be matched by regarding glucose, peptone, cornstarch, ammonium citrate, magnesium sulfate, potassium dihydrogen phosphate as raw material
It sets except the more balanced nutrient solution of all kinds of nutrition, also additional tribulus terrestris, Chinese aloe leaf and the Radix Astragali that certain content is added, and with hydroxyl
Ethyl cellulose coordinates Chinese medicine additive to use, and the Chinese caterpillar fungus culture medium being finally prepared can effectively inhibit varied bacteria growing, add
Fast mycelia molding, enhancing fruiting bodies of cordyceps militaris oxidation resistance, have extremely wide application prospect.
Specific implementation mode
Embodiment is given below so that the present invention to be specifically described, it is necessary to which indicated herein is following embodiment
It is used to further illustrate the present invention, should not be understood as limiting the scope of the invention, the ordinary skill in the field
Personnel still fall within protection scope of the present invention to the nonessential improvement of some made of the invention or adjustment according to this embodiment.
Embodiment 1-6 and comparative example 1-4
The raw material that the Chinese caterpillar fungus culture medium is made includes following components：100 parts of distilled water, 30-70 parts of major ingredient, lepidopterous insects pupa
5-20 parts of powder, 3-12 parts of glucose, 2-8 parts of peptone, 1-5 parts of cornstarch, 0.5-5 parts of ammonium citrate, magnesium sulfate 0.5-5
Part, 0.5-5 parts of potassium dihydrogen phosphate, 7-12 parts of hydroxyethyl cellulose, 15-30 parts of Chinese medicine additive.
Wherein, Chinese medicine additive is made of tribulus terrestris, Chinese aloe leaf and Radix Astragali, the total weight based on Chinese medicine additive, white puncture
The content of lamb's-quarters is 40%-55wt%, the content of Chinese aloe leaf is 20-40wt%, the content of Radix Astragali is 5-25 wt%.
Wherein, lepidopterous insects pupa powder is dried silkworm chrysalis meal.
Wherein, major ingredient is rice.
The method that Chinese caterpillar fungus culture medium is prepared by above-mentioned raw materials is as follows：
（1）Glucose, peptone, cornstarch, ammonium citrate, magnesium sulfate, potassium dihydrogen phosphate are added to the water, battalion is stirred to get
Nutrient solution is spare；
（2）It is added to the water after tribulus terrestris, Chinese aloe leaf and Radix Astragali are mixed, then hydroxyl second is added in boiling 4-6h while stirring
Base cellulose continues to filter after heating 1-1.5h, obtains filtrate and the dregs of a decoction are spare controlled at 50-60 DEG C；
（3）By major ingredient grinds, it then is sequentially added into nutrient solution with lepidopterous insects pupa powder, filtrate, the dregs of a decoction, is stirred evenly,
Obtain mixed liquor；
（4）Mixed liquor is dispensed into culture vessel, and 45min is sterilized under 130 DEG C of high temperature, is cooled to 25 DEG C up to Cordyceps militaris
Culture medium.
The addition of each raw material in embodiment 1-6 and comparative example 1-4（Unit：Parts by weight）It see the table below shown in 1.
Performance data test method
The Chinese caterpillar fungus culture medium being inoculated in super-clean bench in the Cordyceps militaris thalline to embodiment 1-6 and comparative example 1-4 of activation, often
50ml culture medium inoculateds 1mL activates bacterium solution, and other conditions are identical is cultivated to obtain mycelia for control, and mycelia is waited to be used again after growing up to
The medium culture obtains fructification.
Cordyceps militaris mycelia molding time is observed and recorded, and starts culture and occurs visible miscellaneous bacteria bacterium colony in wild Oryza species in 15 days
Number.
DPPH methods measure Cordyceps militaris inoxidizability：When free radical scavenger is added into DPPH free-atom aqueous solutions, orphan is to electricity
Son is paired, and the DPPH free radicals of darkviolet are reduced into yellow DPPH-H non-free radical forms, fading extent with received
Electron amount at quantitative relationship, thus can pass through the variation of absorbance carry out quantitative analysis.Clearance rate is bigger, represents antioxygen
The property changed is better, and assay method is as follows：
（1）It is prepared by Cordyceps militaris runic object：By the fruiting bodies of cordyceps militaris finished product point of 1-6 of the embodiment of the present invention and comparative example 1-4 cultures
It does not crush, by solid-liquid ratio 1：35 extracting in waters, extraction three times, merge extracting solution, are concentrated into certain volume, add 3 times 95% of second
Alcohol precipitates, and takes supernatant concentration, drying, it is spare to obtain Cordyceps militaris crude extract；
（2）In DPPH free-atom aqueous solutions, a concentration of 30mg/ml pupas that are separately added into enough embodiment 1-6 and comparative example 1-4
Cordyceps sinensis crude extract measures clearance rate.
The above test result see the table below shown in 2：
By 2 test result of table it is found that the mycelia molding time of the Cordyceps militaris of the Chinese caterpillar fungus culture medium culture in embodiment 1-6 is 20
Within it, seldom there is miscellaneous bacteria, the clearance rate that DPPH methods measure can reach 75% or more, i.e. Chinese caterpillar fungus culture medium in the present invention
Varied bacteria growing can effectively be inhibited, accelerate mycelia molding, enhancing fruiting bodies of cordyceps militaris oxidation resistance.And the white puncture of comparative example 1-2
Lamb's-quarters, Chinese aloe leaf and Radix Astragali compatibility not in the present invention as defined in particular range, comparative example 3 is not coordinated with hydroxyethyl cellulose
Tribulus terrestris, Chinese aloe leaf and Radix Astragali use, and comparative example 4 finds a large amount of miscellaneous bacterias without addition tribulus terrestris, Chinese aloe leaf and Radix Astragali,
And mycelial growth rate is slower, fruiting bodies of cordyceps militaris inoxidizability is poor, and the test result of various aspects is far inferior to embodiment.Such as
Shown in embodiment 4-6, when the weight ratio between tribulus terrestris, Chinese aloe leaf and Radix Astragali is 5:3:When 2, test result is especially excellent.
Claims (8)
1. a kind of Chinese caterpillar fungus culture medium, using water as solvent, which is characterized in that by weight, the Chinese caterpillar fungus culture medium is made
Raw material includes following components：30-70 parts of major ingredient, 5-20 parts of lepidopterous insects pupa powder, 3-12 parts of glucose, 2-8 parts of peptone, jade
1-5 parts of rice starch, 0.5-5 parts of ammonium citrate, 0.5-5 parts of magnesium sulfate, 0.5-5 parts of potassium dihydrogen phosphate, hydroxyethyl cellulose 7-12
Part, 15-30 parts of Chinese medicine additive, the Chinese medicine additive is made of tribulus terrestris, Chinese aloe leaf and Radix Astragali, based on Chinese medicine additive
Total weight, the content of tribulus terrestris is 40%-55wt%, the content of Chinese aloe leaf is 20-40wt%, the content of Radix Astragali is 5-25 wt%.
2. Chinese caterpillar fungus culture medium according to claim 1, which is characterized in that the content of tribulus terrestris is 50wt%, Chinese aloe leaf
Content is 30wt%, the content of Radix Astragali is 20wt%.
3. Chinese caterpillar fungus culture medium according to claim 2, which is characterized in that the matter of hydroxyethyl cellulose and Chinese medicine additive
Amount is than being 1：2.
4. Chinese caterpillar fungus culture medium according to claim 1, which is characterized in that the major ingredient be selected from rice, wheat, corn or
One or several kinds of mixtures in soya bean.
5. Chinese caterpillar fungus culture medium according to claim 1, which is characterized in that the lepidopterous insects pupa powder is dried silkworm chrysalis meal.
6. Chinese caterpillar fungus culture medium according to claim 1, which is characterized in that the parts by weight of the water are 50-100.
7. according to claim 1-6 any one of them Chinese caterpillar fungus culture mediums, which is characterized in that further include 0.5-5 parts of vitamin,
The vitamin is by vitamin B1, vitamin B2, vitamin B6And vitamin B12Composition.
8. the method for preparing claim 1-7 any one of them Chinese caterpillar fungus culture mediums, which is characterized in that include the following steps：
（1）Glucose, peptone, cornstarch, ammonium citrate, magnesium sulfate, potassium dihydrogen phosphate are added to the water, battalion is stirred to get
Nutrient solution is spare；
（2）It is added to the water after tribulus terrestris, Chinese aloe leaf and Radix Astragali are mixed, then hydroxyl second is added in boiling 4-6h while stirring
Base cellulose continues to filter after heating 1-1.5h, obtains filtrate and the dregs of a decoction are spare controlled at 50-60 DEG C；
（3）By major ingredient grinds, it then is sequentially added into nutrient solution with lepidopterous insects pupa powder, filtrate, the dregs of a decoction, is stirred evenly,
Obtain mixed liquor；
（4）Mixed liquor is dispensed into culture vessel, and 30min-60min is sterilized under 115 DEG C of -140 DEG C of high temperature, is cooled to 25
DEG C up to Chinese caterpillar fungus culture medium.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111657490A (en) * | 2020-06-17 | 2020-09-15 | 北华大学 | Dandelion root saccharified liquid, dandelion root fermentation product, preparation methods of dandelion root saccharified liquid and dandelion root fermentation product, and product containing dandelion root saccharification liquid and dandelion root fermentation product |
| CN111837810A (en) * | 2020-07-27 | 2020-10-30 | 芜湖野树林生物科技有限公司 | Cordyceps militaris culture medium and preparation method and application thereof |
| CN115399192A (en) * | 2022-10-11 | 2022-11-29 | 翔宇药业股份有限公司 | Method for producing cordyceps militaris mycoplasm by using red skin blood replenishing oral liquid dregs and application of cordyceps militaris mycoplasm |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104163685A (en) * | 2014-06-30 | 2014-11-26 | 桂林丰茂源农业技术开发有限公司 | A cordyceps militaris sporophore culture medium comprising Chinese globeflower broken bits |
| CN104311302A (en) * | 2014-10-15 | 2015-01-28 | 合肥路飞农业科技有限公司 | Culture medium of low-cost pleurotus eryngii and preparation method of culture medium |
| CN105503375A (en) * | 2015-12-05 | 2016-04-20 | 正源堂（天津）生物科技有限公司 | Efficient cordyceps militaris strain medium |
-
2018
- 2018-04-12 CN CN201810325965.6A patent/CN108651161A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104163685A (en) * | 2014-06-30 | 2014-11-26 | 桂林丰茂源农业技术开发有限公司 | A cordyceps militaris sporophore culture medium comprising Chinese globeflower broken bits |
| CN104311302A (en) * | 2014-10-15 | 2015-01-28 | 合肥路飞农业科技有限公司 | Culture medium of low-cost pleurotus eryngii and preparation method of culture medium |
| CN105503375A (en) * | 2015-12-05 | 2016-04-20 | 正源堂（天津）生物科技有限公司 | Efficient cordyceps militaris strain medium |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111657490A (en) * | 2020-06-17 | 2020-09-15 | 北华大学 | Dandelion root saccharified liquid, dandelion root fermentation product, preparation methods of dandelion root saccharified liquid and dandelion root fermentation product, and product containing dandelion root saccharification liquid and dandelion root fermentation product |
| CN111837810A (en) * | 2020-07-27 | 2020-10-30 | 芜湖野树林生物科技有限公司 | Cordyceps militaris culture medium and preparation method and application thereof |
| CN115399192A (en) * | 2022-10-11 | 2022-11-29 | 翔宇药业股份有限公司 | Method for producing cordyceps militaris mycoplasm by using red skin blood replenishing oral liquid dregs and application of cordyceps militaris mycoplasm |
| CN115399192B (en) * | 2022-10-11 | 2023-05-23 | 翔宇药业股份有限公司 | Method for producing cordyceps militaris mycoplasm by utilizing red-coated blood-replenishing oral liquid residues and application thereof |
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Application publication date: 20181016 |