CN108642142B - Cartridge assembly and hybridization instrument - Google Patents
Cartridge assembly and hybridization instrument Download PDFInfo
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- CN108642142B CN108642142B CN201810592043.1A CN201810592043A CN108642142B CN 108642142 B CN108642142 B CN 108642142B CN 201810592043 A CN201810592043 A CN 201810592043A CN 108642142 B CN108642142 B CN 108642142B
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- 238000009396 hybridization Methods 0.000 title claims abstract description 27
- 238000006243 chemical reaction Methods 0.000 claims abstract description 115
- 239000007788 liquid Substances 0.000 claims abstract description 15
- 239000002699 waste material Substances 0.000 claims abstract description 11
- 238000003825 pressing Methods 0.000 claims description 9
- 239000003153 chemical reaction reagent Substances 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 8
- 230000009471 action Effects 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 4
- 230000000712 assembly Effects 0.000 claims description 3
- 238000000429 assembly Methods 0.000 claims description 3
- 238000001514 detection method Methods 0.000 description 10
- 238000004140 cleaning Methods 0.000 description 8
- 238000009434 installation Methods 0.000 description 4
- 238000002493 microarray Methods 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- 239000004793 Polystyrene Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000012295 chemical reaction liquid Substances 0.000 description 2
- 230000006835 compression Effects 0.000 description 2
- 238000007906 compression Methods 0.000 description 2
- 238000004043 dyeing Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 244000000010 microbial pathogen Species 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 238000000018 DNA microarray Methods 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 108700019146 Transgenes Proteins 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 239000013566 allergen Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
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Abstract
The invention discloses a cartridge assembly and a hybridization instrument, and relates to the technical field of hybridization instruments. The invention provides a cartridge assembly which comprises a first cartridge, a second cartridge and a reaction chip. The first card box is provided with a reaction bin, a throwing channel and a mounting part, the reaction bin is communicated with the throwing channel, and the reaction chip is arranged in the reaction bin. The second card box is provided with a sample adding port, a window part and an assembling part, wherein the sample adding port is communicated with the reaction bin, the window part is arranged opposite to the reaction bin, and the assembling part is arranged on the side edge of the window part and is propped against the side wall of the reaction bin so as to cover the second card box on the first card box. The invention also provides a hybridization instrument comprising the cartridge assembly. The cartridge component and the hybridization instrument provided by the invention have the characteristics of simple structure, convenience in use and convenience in observation, and can facilitate the treatment of waste liquid.
Description
Technical Field
The invention relates to the technical field of hybridization instruments, in particular to a cartridge assembly and a hybridization instrument.
Background
The traditional microarray biochip analysis system generally comprises several links such as chip hybridization, cleaning, dyeing and detection, which are all manually completed by operators, so that the time and the labor are consumed, experimental errors can be caused by manual operation, and the possibility of pollution exists. Aiming at the problem, scientific researchers develop microarray chip structures of various self-sealing hybridization cleaning systems and complete a full-automatic hybridization cleaning and dyeing integrated system for the microarray chip structures, thereby realizing full automation of operation.
However, such systems today often connect the modules via tubing that allows different reactions or cleaning fluids to pass through the tubing into the cartridge. The liquid can take longer to enter and exit the chip card box through the pipeline, so that the total detection time is prolonged; meanwhile, the pollution is possibly carried out through pipeline transmission; in addition, if the pipeline is improperly selected, the pipeline is easy to age and leak.
Disclosure of Invention
The invention aims to provide a cartridge assembly which has the characteristics of simple structure, convenient use and convenient observation and can facilitate the treatment of waste liquid.
The invention provides a technical scheme about a card box assembly, which comprises the following steps:
A cartridge assembly comprises a first cartridge, a second cartridge and a reaction chip. The first card box is provided with a reaction bin, a throwing-out channel and an installation part, wherein the reaction bin is communicated with the throwing-out channel, and the reaction chip is arranged in the reaction bin. The second card box is provided with a sample adding port, a window part and an assembly part, wherein the sample adding port is communicated with the reaction bin, the window part is opposite to the reaction bin, and the assembly part is arranged on the side edge of the window part and is propped against the side wall of the reaction bin so as to cover the second card box on the first card box.
Further, the window part is protruded towards the direction of the reaction bin and a concave part is formed at one side of the second card box away from the reaction bin.
Further, the assembly portion is kept away from the one end tip of second card box is provided with the compression face, the reaction chip set up in the diapire of reaction storehouse, just the compression face with the reaction chip supports and holds.
Further, the end part of the assembling part far away from one end of the second card box is provided with a bulge, the pressing surface extends to the bulge, and the side wall of the reaction bin is provided with a groove matched with the bulge.
Further, the first cartridge is further provided with an inlet channel, the inlet channel is communicated with the reaction bin, and the sample adding port is opposite to the inlet channel and is communicated with the inlet channel.
Further, the bottom surface of the inlet channel is obliquely arranged towards the reaction bin, and the position of the mounting part is opposite to the position of the inlet channel.
Further, the second cartridge is further provided with a marking portion, and the position of the marking portion is opposite to the throwing-out channel.
Further, the second cartridge is further provided with a flow limiting strip, the position of the flow limiting strip is opposite to that of the identification part, and the flow limiting strip is matched with the throwing channel.
Further, the number of the first cartridges and the number of the second cartridges are corresponding to a plurality of the first cartridges, and the plurality of the first cartridges are sequentially connected.
Another object of the present invention is to provide a hybridization apparatus which has the characteristics of simple structure, convenient use and convenient observation, and can facilitate the treatment of waste liquid.
The invention also provides a technical scheme about the hybridization instrument:
A hybridization apparatus includes a plurality of cartridge assemblies. The cartridge assembly comprises a first cartridge, a second cartridge and a reaction chip. The first card box is provided with a reaction bin, a throwing-out channel and an installation part, wherein the reaction bin is communicated with the throwing-out channel, and the reaction chip is arranged in the reaction bin. The second card box is provided with a sample adding port, a window part and an assembly part, wherein the sample adding port is communicated with the reaction bin, the window part is opposite to the reaction bin, and the assembly part is arranged on the side edge of the window part and is propped against the side wall of the reaction bin so as to cover the second card box on the first card box. The plurality of first cassettes are connected in sequence.
Compared with the prior art, the cartridge assembly and the hybridization instrument provided by the invention have the beneficial effects that:
the reaction bin arranged on the first card box is used for containing reaction liquid, and the reaction chip is arranged in the reaction bin, that is, the reaction bin provides a place for reaction. The second clamping box is used for covering the reaction bin, and the sample adding port is used for adding reaction liquid into the reaction bin. The throwing channel is used for throwing out the reacted waste liquid from the throwing channel under the action of centrifugal force, and the throwing channel is a micro-flow channel, which can also prevent the reagent from being thrown out (centrifugal force is different) in the process of evenly mixing the reagent. The window part is used for facilitating observation, and the assembly part is used for being detachably connected with the first box body. The mounting part is used for mounting the first box body on the hybridization instrument. The cartridge component and the hybridization instrument provided by the invention have the characteristics of simple structure, convenience in use and convenience in observation, and can facilitate the treatment of waste liquid.
Drawings
In order to more clearly illustrate the technical solution of the embodiments of the present invention, the drawings that are required to be used in the embodiments will be briefly described. It is appreciated that the following drawings depict only certain embodiments of the invention and are therefore not to be considered limiting of its scope. Other relevant drawings may be made by those of ordinary skill in the art without undue burden from these drawings.
FIG. 1 is a schematic view of a cartridge assembly according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of a cartridge assembly according to an embodiment of the present invention in a second view;
FIG. 3 is an exploded view of a cartridge assembly according to an embodiment of the present invention;
Fig. 4 is an enlarged schematic view of the structure at IV in fig. 2.
Icon: a 100-cartridge assembly; 110-a first cartridge; 112-a reaction bin; 114-fling out the channel; 116-mounting part; 118-access channel; 120-a second cartridge; 121-a sample addition port; 123-window section; 125-fitting part; 127-identification; 128-a flow-restricting bar; 130-reaction chip.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. It will be apparent that the described embodiments are some, but not all, embodiments of the invention. The components of the embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the invention, as presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.
In the description of the present invention, it should be understood that the directions or positional relationships indicated by the terms "upper", "lower", "inner", "outer", "left", "right", etc. are based on the directions or positional relationships shown in the drawings, or the directions or positional relationships conventionally put in place when the inventive product is used, or the directions or positional relationships conventionally understood by those skilled in the art are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the apparatus or elements referred to must have a specific direction, be configured and operated in a specific direction, and therefore should not be construed as limiting the present invention.
Furthermore, the terms "first," "second," and the like, are used merely to distinguish between descriptions and should not be construed as indicating or implying relative importance.
In the description of the present invention, it should also be noted that, unless explicitly specified and limited otherwise, terms such as "disposed," "connected," and the like are to be construed broadly, and for example, "connected" may be either fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art according to the specific circumstances.
The following describes specific embodiments of the present invention in detail with reference to the drawings.
First embodiment
Referring to fig. 1 to 4, the present embodiment provides a cartridge assembly 100, which has the characteristics of simple structure, convenient use and convenient observation, and can facilitate the treatment of waste liquid.
It should be noted that the cartridge assembly 100 provided in this embodiment is used in a hybridization apparatus, such as molecular hybridization, protein hybridization, and the like. The cartridge assembly 100 functions to implement a reaction performed by the reaction chip 130 disposed therein.
Meanwhile, it is also required to explain that the gene film chip detection method is used for detecting animal-derived components, pathogenic microorganisms, plant-derived components, transgenes, allergen components and the like. The method is characterized in that: simple, practical, quick and accurate, and has the characteristics of low cost, full detection index, direct interpretation by naked eyes and the like. The detection method can realize single-reaction multi-index detection, and compared with the common PCR method and the fluorescent quantitative PCR method, the detection method greatly reduces the labor intensity of users and reduces the detection time. The introduction of the method can improve the level of relevant scientific researches on food-borne plants, animals, pathogenic microorganisms and the like by functional departments, and has certain significance for improving the detection capability of laboratories. The reaction chip 130 used in this embodiment is a membrane chip, that is, the hybridization apparatus provided in this embodiment can implement the gene membrane chip detection method.
The cartridge assembly 100 provided in this embodiment includes a first cartridge 110, a second cartridge 120, and a reaction chip 130. The first cartridge 110 is provided with a reaction chamber 112, a throwing channel 114 and an installation part 116, the reaction chamber 112 is communicated with the throwing channel 114, and the reaction chip 130 is arranged in the reaction chamber 112. The second cartridge 120 is provided with a sample loading port 121, a window portion 123 and an assembling portion 125, the sample loading port 121 is communicated with the reaction chamber 112, the window portion 123 is opposite to the reaction chamber 112, and the assembling portion 125 is disposed at a side edge of the window portion 123 and abuts against a side wall of the reaction chamber 112, so as to cover the second cartridge 120 on the first cartridge 110.
It should be noted that, the reaction chamber 112 provided in the first cartridge 110 is used for accommodating the reaction solution, and the reaction chip 130 is disposed in the reaction chamber 112, that is, the reaction chamber 112 provides a place for reaction. The second cartridge 120 is used for covering the reaction chamber 112, and the sample inlet 121 is used for adding the reaction solution into the reaction chamber 112. The throwing channel 114 is used for throwing out the reacted waste liquid from the throwing channel 114 under the action of centrifugal force, and the throwing channel 114 is a micro-flow channel, which can also prevent the reagent from being thrown out (centrifugal force is different) in the process of mixing the reagents. The window portion 123 serves to facilitate viewing, and the fitting portion 125 is used to detachably connect with the first casing. The mounting portion 116 is used to mount the first cartridge to a hybridization apparatus.
In this embodiment, the window 123 protrudes toward the reaction chamber 112 and forms a recess on a side of the second cartridge 120 away from the reaction chamber 112.
That is, the window portion 123 is disposed to be submerged, so that the window portion 123 is further closer to the bottom surface of the reaction chamber 112, and thus the cleaning solution does not need to fill the reaction chamber 112, and only the cleaning solution level needs to be in contact with the submerged inner surface of the window. The arrangement of the concave portion can prevent the overflow of the cleaning solution in the reaction chamber 112 from being easily caused by too much cleaning solution.
In this embodiment, a pressing surface is disposed at an end of the assembly portion 125 away from the second cartridge 120, the reaction chip 130 is disposed on the bottom wall of the reaction chamber 112, and the pressing surface abuts against the reaction chip 130.
It can be appreciated that the reaction chip 130 is disposed on the bottom surface of the reaction chamber 112, and is fixed by pressing the reaction chip 130 to prevent the reaction chip 130 from moving and being thrown out. Meanwhile, the pressing surface is arranged on the assembling part 125, so that the structure is simpler, and the assembly is more convenient and faster.
In this embodiment, the end of the fitting portion 125 away from the second cartridge 120 is provided with a protrusion, the pressing surface extends to the protrusion, and the sidewall of the reaction chamber 112 is provided with a groove that mates with the protrusion.
That is, the fitting portion 125 is better snap-fitted by the projection and the groove. Of course, not limited thereto, in other embodiments of the present invention, the assembling portion 125 may also cooperate with the dimension relationship between the side walls of the reaction chamber 112, such as the dimension of the assembling portion 125 is slightly larger than the distance between the side walls of the reaction chamber 112, and further the assembling portion 125 is clamped between the two side walls of the reaction chamber 112 by the displacement of the assembling portion 125 itself.
In this embodiment, the first cartridge 110 is further provided with an inlet channel 118, the inlet channel 118 communicates with the reaction chamber 112, and the sample inlet 121 is opposite to the inlet channel 118 and communicates with the inlet channel 118.
Further, in the present embodiment, the bottom surface of the inlet channel 118 is inclined toward the reaction chamber 112, and the position of the mounting portion 116 is opposite to the position of the inlet channel 118.
In this embodiment, the second cartridge 120 is further provided with a marking portion 127, and the marking portion 127 is located opposite to the throwing-out passage 114.
It should be noted that, the function of the identification portion 127 is to set an identification, so that the identification portion 127 that can be used to identify the type of chip is provided at the end of the cartridge assembly 100 in order to automatically identify the type of chip in the cartridge assembly 100 during the image capturing stage.
Alternatively, the identification part 127 may be provided in the form of an array of spots, each of which may be divided into two colors of black and white, corresponding to 0 and 1 in the computer, and the type of chip can be determined by the specific form of the spot at the image capturing stage.
In addition, a mark consisting of a letter without brackets and a number can be arranged below the sample application mark, and the mark is mainly used for identifying the reaction chip 130 of the cartridge assembly 100 by an operator.
The indicia may be provided using a paper with a backing adhesive and then printed on its surface with the desired application, letters and numbers using a printer. The label can be pasted quantitatively by a producer according to the types before being delivered to a customer; the required identification can also be delivered to the customer together with the card box, so that the customer can paste the identification according to the requirement.
In this embodiment, the second cartridge 120 is further provided with a flow-limiting strip 128, the flow-limiting strip 128 being located opposite the identification portion 127, the flow-limiting strip 128 being engaged with the slinging channel 114.
It should be noted that, the flow-limiting strip 128 cooperates with the throwing-out channel 114 to prevent the thrown-out liquid from entering between the first cartridge 110 and the second cartridge 120 and splashing, and the flow-limiting strip 128 can prevent the liquid from splashing everywhere, so that the liquid only flows out of the throwing-out channel 114.
In the present embodiment, the number of the first cartridges 110 and the second cartridges 120 is a corresponding plurality, and the plurality of first cartridges 110 are sequentially connected.
It should be noted that, the connection between the plurality of first cartridges 110 may be detachable connection or may be integrally formed, and when only one of the first cartridges 110 is needed, it may be removed by a detachable connection manner; when the plurality of first cartridges 110 are connected in an integrally formed manner, the plurality of first cartridges 110 may be separated by shearing or breaking according to the manufacturing material.
Alternatively, the cartridge assembly 100 is made of PS (polystyrene plastic) material, which has the characteristic of high transparency, and can facilitate image acquisition of experimental results.
The beneficial effects of the cartridge assembly 100 provided in this embodiment are: the reaction chamber 112 provided in the first cartridge 110 is used for accommodating a reaction solution, and the reaction chip 130 is disposed in the reaction chamber 112, that is, the reaction chamber 112 provides a reaction place. The second cartridge 120 is used for covering the reaction chamber 112, and the sample inlet 121 is used for adding the reaction solution into the reaction chamber 112. The throwing channel 114 is used for throwing out the reacted waste liquid from the throwing channel 114 under the action of centrifugal force, and the throwing channel 114 is a micro-flow channel, which can also prevent the reagent from being thrown out (centrifugal force is different) in the process of mixing the reagents. The window portion 123 serves to facilitate viewing, and the fitting portion 125 is used to detachably connect with the first casing. The mounting portion 116 is used to mount the first cartridge to a hybridization apparatus. The cartridge assembly 100 provided in this embodiment has the characteristics of simple structure, convenient use and convenient observation, and can facilitate the treatment of waste liquid.
Second embodiment
Referring to fig. 1 to 4, the present embodiment provides a hybridization apparatus, which has the characteristics of simple structure, convenient use and convenient observation, and can facilitate the treatment of waste liquid.
The hybridization apparatus provided in this embodiment includes a base, a rotating disk, and a plurality of cartridge assemblies 100 disposed on the rotating disk. The rotating disk is rotatably disposed on the base, and the cartridge assembly 100 includes a first cartridge 110, a second cartridge 120, and a reaction chip 130. The first cartridge 110 is provided with a reaction chamber 112, a throwing channel 114 and an installation part 116, the reaction chamber 112 is communicated with the throwing channel 114, and the reaction chip 130 is arranged in the reaction chamber 112. The second cartridge 120 is provided with a sample loading port 121, a window portion 123 and an assembling portion 125, the sample loading port 121 is communicated with the reaction chamber 112, the window portion 123 is opposite to the reaction chamber 112, and the assembling portion 125 is disposed at a side edge of the window portion 123 and abuts against a side wall of the reaction chamber 112, so as to cover the second cartridge 120 on the first cartridge 110. The plurality of first cartridges 110 are sequentially connected.
The above description is only of the preferred embodiments of the present invention and is not intended to limit the present invention, but various modifications and variations can be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (5)
1. The cartridge assembly is characterized by comprising a first cartridge, a second cartridge and a reaction chip;
The first cartridge is provided with a reaction bin, a throwing channel and a mounting part, the reaction bin is communicated with the throwing channel, the reaction chip is arranged in the reaction bin, and the mounting part is used for mounting the first cartridge on a hybridization instrument;
The second clamping box is provided with a sample adding port, a window part and an assembling part, the sample adding port is communicated with the reaction bin, the window part is arranged opposite to the reaction bin, and the assembling part is arranged on the side edge of the window part and is propped against the side wall of the reaction bin so as to enable the second clamping box to be covered on the first clamping box;
the second card box is also provided with a marking part, and the position of the marking part is opposite to the throwing channel;
The second card box is also provided with a current limiting strip, the current limiting strip is opposite to the position of the identification part, and the current limiting strip is matched with the throwing channel;
The window part is convexly arranged towards the direction of the reaction bin, and a concave part is formed at one side of the second card box away from the reaction bin;
The first cartridge is further provided with an inlet channel which is communicated with the reaction bin, and the sample adding port is opposite to the inlet channel and is communicated with the inlet channel;
the bottom surface of the entering channel is obliquely arranged towards the reaction bin, and the position of the mounting part is opposite to the position of the entering channel;
The throwing channel is used for throwing out the reacted waste liquid under the action of centrifugal force, and is a micro-flow channel, and according to the difference of centrifugal force, the reagent can be prevented from being thrown out in the process of evenly mixing the reagent.
2. The cartridge assembly according to claim 1, wherein an end portion of the fitting portion remote from the second cartridge is provided with a pressing surface, the reaction chip is disposed on a bottom wall of the reaction chamber, and the pressing surface abuts against the reaction chip.
3. The cartridge assembly according to claim 2, wherein an end of the fitting portion remote from the second cartridge is provided with a projection, the pressing surface extends to the projection, and a side wall of the reaction cartridge is provided with a groove fitted with the projection.
4.A cartridge assembly according to any one of claims 1-3, wherein the number of the first cartridges and the second cartridges is a corresponding plurality, and a plurality of the first cartridges are connected in sequence.
5. A hybridization apparatus comprising a plurality of cartridge assemblies according to any one of claims 1-4, wherein a plurality of said first cartridges are connected in sequence.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810592043.1A CN108642142B (en) | 2018-06-08 | 2018-06-08 | Cartridge assembly and hybridization instrument |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810592043.1A CN108642142B (en) | 2018-06-08 | 2018-06-08 | Cartridge assembly and hybridization instrument |
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| CN108642142A CN108642142A (en) | 2018-10-12 |
| CN108642142B true CN108642142B (en) | 2024-06-21 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109576150A (en) * | 2018-12-29 | 2019-04-05 | 西南民族大学 | Membrane DNA chip hybridization instrument |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203830030U (en) * | 2014-04-04 | 2014-09-17 | 北京贝泰科技有限公司 | Multi-target micro array chip full-automatic hybridization washing dyeing system |
| CN107102131A (en) * | 2017-07-03 | 2017-08-29 | 沈阳微流控生物科技有限公司 | A kind of full-automatic micro-fluidic chip fluorescence immunoassay detecting system and its detection method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6258593B1 (en) * | 1999-06-30 | 2001-07-10 | Agilent Technologies Inc. | Apparatus for conducting chemical or biochemical reactions on a solid surface within an enclosed chamber |
| CN2725302Y (en) * | 2004-09-07 | 2005-09-14 | 中国人民解放军第三军医大学 | Multipurpose biological macromolecule microarray chip hybridization instrument |
| CN204544220U (en) * | 2015-03-30 | 2015-08-12 | 博奥生物集团有限公司 | A kind of rotating centrifugal separation of whole blood chip |
| CN106179549B (en) * | 2016-08-15 | 2020-11-17 | 南京大学 | Micro-fluidic chip and application thereof |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203830030U (en) * | 2014-04-04 | 2014-09-17 | 北京贝泰科技有限公司 | Multi-target micro array chip full-automatic hybridization washing dyeing system |
| CN107102131A (en) * | 2017-07-03 | 2017-08-29 | 沈阳微流控生物科技有限公司 | A kind of full-automatic micro-fluidic chip fluorescence immunoassay detecting system and its detection method |
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