CN2725302Y - Multipurpose biological macromolecule microarray chip hybridization instrument - Google Patents
Multipurpose biological macromolecule microarray chip hybridization instrument Download PDFInfo
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- CN2725302Y CN2725302Y CNU2004200611670U CN200420061167U CN2725302Y CN 2725302 Y CN2725302 Y CN 2725302Y CN U2004200611670 U CNU2004200611670 U CN U2004200611670U CN 200420061167 U CN200420061167 U CN 200420061167U CN 2725302 Y CN2725302 Y CN 2725302Y
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Abstract
The utility model relates to a multi-purpose hybridization instrument for a biological-macromolecule micro-array chip, which comprises a hybridization chamber 2 and a sealing cover 10, wherein the sealing cover which can completely seal the hybridization chamber is made of heat-resisting transparent material; raised sheet seats 5 are arrayed at the bottom of the hybridization chamber which is made of metal with good heat-conducting property; fluid slots 7 which are mutually communicated are arranged among the sheet seats; a fluid feeding hole which is communicated with the hybridization chamber is arranged on the outer wall of the hybridization chamber; the upper end of the fluid feeding hole is higher than the maximum liquid level position of the hybridization chamber; the lower end of the fluid feeding hole is positioned at the bottom of the hybridization chamber is connected with the fluid bath; the bottom surface of the hybridization instrument is provided with a positioning slot 8. The hybridization instrument is convenient to control the temperature and humidity of the hybridization of the biological-macromolecule micro-array chip and is convenient to control the temperature in the process of cleaning after the hybridization of the biological-macromolecule micro-array chip, avoiding to directly flush the hybridization instrument for the biological-macromolecule micro-array chip at the surface of a chip. The utility model can also be used for experiments such as in situ hybridization, in situ polymerase china reaction, immunohistochemistry staining, etc.
Description
Affiliated technical field
The utility model relates to a kind of device that is used for the biomacromolecule microarray hybridization, the temperature when this device can effectively be controlled the biomacromolecule microarray hybridization, and chip is in the suitable humidity when guaranteeing hybridization.Can also control the temperature of hybridization last handling process by this device, and can avoid because the inhomogenous problem of hybridization signal that inhomogeneous cleaning causes.This utility model also can be used for experiments such as in situ hybridization, original position PCR and immunohistochemical methods.
Background technology
The biomacromolecule micro-array chip is a kind of high-throughput biomacromolecule detection technology that development in recent years is got up.Its ultimate principle is to form array the fixing a considerable amount of biomacromolecules (as nucleic acid molecule, protein molecule, polysaccharide molecule etc.) in solid-phase matrix (present modal solid-phase matrix is a slide glass) surface, each point in the array is exactly a probe, the diameter of each point is remembered with micron, therefore can prepare the micro-array chip that contains up to ten thousand different probes; The sample that will contain multiple molecules detected then is added on the chip hybridizes, and by detecting hybridization signal, just can determine the situation of molecules detected in the sample.Utilize the biomacromolecule micro-array chip can detect the nearly target molecule more than ten thousand kinds simultaneously, greatly improved detection efficiency, and the information that can provide ordinary method to provide.At present the most frequently used biomacromolecule micro-array chip is by with the method for biomacromolecule with pin mark or specking, puts the surface through preparing on the slide glass of special processing.This class chip technology of preparing is relatively easy, product price cheap, subsequent operations is comparatively simple, thereby has obtained using widely.
Above-mentioned biomacromolecule micro-array chip also exposes some problems in application, outstanding is that repeatability is bad.Influence the biomacromolecule micro-array chip factor that mainly contains three aspects of repeatability as a result: the preparation of chip, the preparation of sample and hybridization detect.This its chips preparation is the easiest to be standardized; The biological specimen preparation is because accumulation for many years also has some mature methods to carry out quality control; And also very not insufficient to the crossover process people's of chip understanding, also be that room for improvement is arranged most.Our work shows: the treatment process after the hybridization has great influence to the result.Most importantly will not have and probe bonded target molecule with suitable washing lotion flush away after the hybridization, our result shows that the composition of washing lotion, temperature, cleaning way all can influence the result; The temperature height was once with low once the result was significantly different; During cleaning in same cleaning cylinder the chip results of different positions also different.
In situ hybridization, original position PCR and immunohistochemical methods etc. are the experimental techniques of widespread use in biology, the medical research, all need controlled temperature and humidity in operating process.
Summary of the invention
Technical problem to be solved in the utility model provides and a kind ofly can make chip hybridization and the standardized biomacromolecule microarray hybridization of purging method instrument, temperature when this hybridization instrument can effectively be controlled the biomacromolecule microarray hybridization, chip is in the saturated humidity when guaranteeing hybridization, avoids the dry hybridization failure that causes of hybridization solution.Can also control the temperature of hybridization back cleaning process by this device, and can avoid because the inhomogenous problem of hybridization signal that inhomogeneous cleaning causes.Utilize this device can also finish experiments such as in situ hybridization, original position PCR and immunohistochemical methods.
The technical scheme that its technical problem that solves the utility model adopts is such: promptly a kind of many with biomacromolecule microarray hybridization instrument, it comprise the hybridization chamber of making by the metal of good heat conductivity and hybridization chamber can be sealed fully, with sealing cover heat-resisting, the transparent material making; Wherein, the hybridization chamber bottom is arranged with the bar of projection, has the liquid bath that is interconnected between the bar, has the liquid filling hole that is communicated with hybridization chamber on the hybridization chamber outer wall, the upper end of liquid filling hole is higher than hybridization chamber maximum fluid position, and the bottom that the lower end of liquid filling hole is positioned at hybridization chamber links to each other with liquid bath; Bottom surface at hybridization instrument has locating slot.
In situ hybridization is with the target molecule in the nucleic acid probe detection tissue slice, utilizes said apparatus can reach the purpose of control hybridization humidity and temperature, finishes hybridization in situ experiment.Its implementation process is identical with the said chip crossover process.
Original position PCR is in the enterprising performing PCR reaction of tissue slice, utilizes said apparatus, by its locating slot at bottom, is fixed on the heating module of heating instrument, can carry out original position PCR experiment.Its process is similar to the said chip crossover process, but temperature can constantly change according to requirement of experiment in the experiment.
Immunohistochemical methods is with the target molecule in antibody or the Detection of antigen tissue slice, and the humidity when utilizing said apparatus can reach the control antigen antibody reaction and the purpose of temperature are finished immunohistochemical experiment.Its implementation process is identical with the said chip crossover process.
The beneficial effects of the utility model are, the temperature in the time of can effectively controlling the biomacromolecule microarray hybridization, and chip is in the saturated humidity when guaranteeing hybridization, avoids the dry hybridization failure that causes of hybridization solution.Can also control the temperature of hybridization back cleaning process by this device, and can avoid because the inhomogenous problem of hybridization signal that inhomogeneous cleaning causes.The utility model effect is obvious, and is easy to use, and multiple use can be arranged; Simple in structure, reliable, no easily worn part; Material is cheap, is easy to processing.
Description of drawings
Below in conjunction with drawings and Examples the utility model is further specified.
Fig. 1-the 6th, structure iron of the present utility model; Wherein
Fig. 1 removes vertical view behind the sealing cover for the utility model;
Fig. 2 is the A-A sectional view of Fig. 1;
Fig. 3 is a upward view of the present utility model;
Fig. 4 is the utility model sealing cover vertical view;
Fig. 5 is the B-B sectional view of Fig. 4;
Fig. 6 is the sectional view of sealing plug 12;
Fig. 7 is the diagrammatic cross-section of the utility model user mode 1;
Fig. 8 is the diagrammatic cross-section of the utility model user mode 2.
1: housing; 2: hybridization chamber; 3: liquid filling hole; 4: screw; 5: bar; 6: sealing groove; 7: liquid bath; 8: locating slot; 9: seal washer; 10: sealing cover; 11: retaining screw; 12: sealing plug; 13: the biomacromolecule micro-array chip; 14: cover glass; 15: tissue slice.
Embodiment
The embodiment that provides below by accompanying drawing further specifies the utility model.
Referring to accompanying drawing 1-6, many among the figure with biomacromolecule microarray hybridization instrument, comprise the hybridization chamber 2 of making by the metal of good heat conductivity and hybridization chamber can be sealed fully, with sealing cover 10 heat-resisting, the transparent material making; Wherein, the hybridization chamber bottom is arranged with the bar 5 of projection, has the liquid bath 7 that is interconnected between the bar, has the liquid filling hole 3 that is communicated with hybridization chamber on the hybridization chamber outer wall, the upper end of liquid filling hole is higher than hybridization chamber maximum fluid position, and the bottom that the lower end of liquid filling hole is positioned at hybridization chamber links to each other with liquid bath; Has locating slot 8 in the bottom surface of hybridization instrument.
In an embodiment, the top of hybridization chamber 2 has screw 4 and annular seal groove 6, and sealing groove is embedded with seal washer 9, and sealing cover 10 cooperates with screw 4 and seal washer 9 by screw 11 and hybridization chamber is sealed.
Referring to accompanying drawing 2, the hybridization chamber among the embodiment has two liquid filling holes 3, and the position that the bottom that the lower end of liquid filling hole 3 is positioned at hybridization chamber is lower than liquid bath 7 links to each other with liquid bath 7.Described liquid filling hole 3 has sealing plug 12.The opening section of liquid filling hole upper end is up big and down small trapezoidal.
The novel concrete use-case of this use is referring to Fig. 7: earlier hybridization solution is added to chip 13 surfaces, covered 14; Chip is placed on the bar 5; In sealing groove 6, put into seal washer 9; Sealing cover 10 is covered on hybridization chamber 2; With four retaining screws 11 sealing cover is fixed on the housing by screw 4, seals hybridization chamber simultaneously; Add less water by liquid filling hole 3 in hybridization chamber, its liquid level is not higher than bar 5 end faces; With sealing plug 12 sealing liquid filling holes; The hybridization instrument that assembles is placed on the heating instrument heating module, or horizontal positioned (water-bath, water bath, temperature control shaking table, incubator etc.) in other suitable heating appliances; After hybridization under the suitable temperature, open sealing cover, remove the cover glass on the chip, remove water, again chip is put back to hybridization chamber, reseal; Open sealing plug, steadily add washing lotion, be full of hybridization chamber, steadily extract washing lotion (composition of washing lotion and the number of times of cleaning are determined according to trial test) again out, open hybridization chamber, take out chip and can carry out next step operation with injector for medical purpose.
Novel another the concrete use-case of this use is referring to Fig. 8: after hybridization under the suitable temperature, open sealing cover 10, remove the cover glass on the chip, remove water, chip 13 is put back in the hybridization chamber 2 again, reseal; Open sealing plug 12, steadily add washing lotion by liquid filling hole, be full of hybridization chamber 2, steadily extract washing lotion (composition of washing lotion and the number of times of cleaning are determined according to trial test) again out, open hybridization chamber 2, take out chip 13 and can carry out next step operation with injector for medical purpose.
The utility model is owing to utilize the metallic substance of good heat conductivity, process one and can put several chip hybridization chambers, by sealing cover and a sealing groove and a seal washer that usefulness is heat-stable, transparent material is made, hybridization chamber can be sealed fully blocking-up hybridization chamber and extraneous generation liquid, gas exchange; The sealing cover of making of transparent material can make situation in operator's clear view hybridization chamber.Metal lug seat in the bottom of hybridization chamber is convenient to place chip.The effect one that forms some liquid baths between the bar is can add some liquid when hybridization, make the hybridization chamber of sealing in high-temp hybridized process, keep saturated humidity, prevent that hybridization solution is dry, the existence of liquid bath can avoid the liquid of the maintenance humidity that adds to mix with hybridization solution; The 2nd, in the cleaning process after hybridization, play a part to make washing lotion evenly to flow into, flow out hybridization chamber.The liquid feeding end of liquid filling hole is higher than the maximum fluid of hybridization chamber, makes that the liquid that can not in the hybridization chamber flows out, and can also it be sealed fully with the sealing plug that matches with it; The bottom that the outlet end of liquid filling hole is positioned at hybridization chamber links to each other with liquid bath, makes the washing lotion that flows into, flows out hybridization chamber can directly not wash chip surface, has farthest avoided the inhomogeneous of chip cleaning.The liquid filling hole opening that is positioned at housing is in the opening part slightly larger in diameter, and the subsequent portion diameter is less, and smaller portions diameter and injector for medical purpose nipple geometrical dimension match; This design has three purposes: the firstth, increase stopping property, the second, can make sealing plug can projection on the hybridization instrument surface, conveniently on some have the heating instrument of lid, use the chip hybridization instrument, the 3rd syringe easy to use adds, extracts out liquid.Be provided with when two liquid filling holes make by a liquid filling hole adding or extraction liquid and can discharge or suck air by another liquid filling hole.The locating slot 8 that has in the bottom surface of hybridization instrument, its size can determine that locating slot makes hybridization instrument more tight with contacting of heating module, also prevents the hybridization instrument displacement, also helps thermal conduction simultaneously to bar according to the size of the heating instrument heating module that will use.
Claims (5)
1, a kind of many with biomacromolecule microarray hybridization instrument, it is characterized in that: comprise the hybridization chamber (2) of making by the metal of good heat conductivity and hybridization chamber can be sealed fully, with sealing cover (10) heat-resisting, the transparent material making; Wherein, the hybridization chamber bottom is arranged with the bar (5) of projection, has the liquid bath (7) that is interconnected between the bar, on the hybridization chamber outer wall, has the liquid filling hole (3) that is communicated with hybridization chamber, the upper end of liquid filling hole is higher than hybridization chamber maximum fluid position, and the bottom that the lower end of liquid filling hole is positioned at hybridization chamber links to each other with liquid bath; Has locating slot (8) in the bottom surface of hybridization instrument.
2, according to claim 1 many with biomacromolecule microarray hybridization instrument, it is characterized in that: the top of hybridization chamber (2) has screw (4) and annular seal groove (6), sealing groove is embedded with seal washer (9), and sealing cover (10) cooperates with screw (4) and seal washer (9) by screw (11) and hybridization chamber is sealed.
3, according to claim 1 many it is characterized in that: hybridization chamber has two liquid filling holes (3) with biomacromolecule microarray hybridization instrument, and the position that the bottom that the lower end of liquid filling hole (3) is positioned at hybridization chamber is lower than liquid bath (7) links to each other with liquid bath (7).
4, according to claim 1 many with biomacromolecule microarray hybridization instrument, it is characterized in that: described liquid filling hole (3) has sealing plug (12).
5, according to claim 4 many with biomacromolecule microarray hybridization instrument, it is characterized in that: the opening section of liquid filling hole (3) upper end is up big and down small trapezoidal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2004200611670U CN2725302Y (en) | 2004-09-07 | 2004-09-07 | Multipurpose biological macromolecule microarray chip hybridization instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2004200611670U CN2725302Y (en) | 2004-09-07 | 2004-09-07 | Multipurpose biological macromolecule microarray chip hybridization instrument |
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| Publication Number | Publication Date |
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| CN2725302Y true CN2725302Y (en) | 2005-09-14 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNU2004200611670U Expired - Fee Related CN2725302Y (en) | 2004-09-07 | 2004-09-07 | Multipurpose biological macromolecule microarray chip hybridization instrument |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108642142A (en) * | 2018-06-08 | 2018-10-12 | 四川华汉三创生物科技有限公司 | Cartridge component and hybridization instrument |
-
2004
- 2004-09-07 CN CNU2004200611670U patent/CN2725302Y/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108642142A (en) * | 2018-06-08 | 2018-10-12 | 四川华汉三创生物科技有限公司 | Cartridge component and hybridization instrument |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |