CN108635521A - A kind of topical composition and preparation method thereof for treating burn and scald - Google Patents

A kind of topical composition and preparation method thereof for treating burn and scald Download PDF

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CN108635521A
CN108635521A CN201810748150.9A CN201810748150A CN108635521A CN 108635521 A CN108635521 A CN 108635521A CN 201810748150 A CN201810748150 A CN 201810748150A CN 108635521 A CN108635521 A CN 108635521A
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filtrate
supernatant
composition
decocted
hours
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李士洋
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    • A—HUMAN NECESSITIES
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
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    • A61K36/185—Magnoliopsida (dicotyledons)
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    • A61K36/24—Apocynaceae (Dogbane family), e.g. plumeria or periwinkle
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    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/32—Burseraceae (Frankincense family)
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/60—Moraceae (Mulberry family), e.g. breadfruit or fig
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    • A61K36/888—Araceae (Arum family), e.g. caladium, calla lily or skunk cabbage
    • A61K36/8884—Arisaema, e.g. Jack in the pulpit
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    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
    • A61K47/10—Alcohols; Phenols; Salts thereof, e.g. glycerol; Polyethylene glycols [PEG]; Poloxamers; PEG/POE alkyl ethers
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    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0014—Skin, i.e. galenical aspects of topical compositions
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    • A61K9/00—Medicinal preparations characterised by special physical form
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    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
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Abstract

The present invention discloses a kind of topical composition and preparation method thereof for treating burn and scald.The composition is made of the 13 taste bulk pharmaceutical chemicals such as rough melic herb, daghestan sweetclover herb, the root bark of white mulberry, cicada fungus, hawksbill turtle, Folium Apocyni Veneti, Semen Cuscutae, rhizoma arisaematis, corydalis tuber, kaladana, frankincense, myrrh, centella.The advantage of the invention is that selected bulk pharmaceutical chemicals compatibility is suitable, meet traditional Chinese medicine and pharmacy and modern medical theory, and with heat-clearing toxin expelling, anti-inflammatory analgetic, removing necrosis, promoting granulation, anti-infective, promote wound healing fast, preventing from scar after healing, therapeutic effect is notable.

Description

A kind of topical composition and preparation method thereof for treating burn and scald
Technical field
The present invention relates to field of biological pharmacy, more particularly to a kind of topical composition for treating burn and scald and its preparation side Method.
Background technology
Burn and scald is common unexpected injury in life, boiling water, rolling congee, deep fat, heat steam burning scald and can often occur Thing.For deep lesions, since skin damage is than more serious, the scars such as incrustation are always left after treatment, not only influence the wounded day Appearance afterwards, also because of position pore-free of forming a scab, the toxin such as sweat can not be discharged at the position and many new diseases occur.It is existing The drug and method for treating burn and scald, can usually form many incrustations, influence the image of the wounded, spiritual wound can be left to the wounded Evil.Undesirable consequence would not be caused if processing is not in time to these burn and scalds.Although burn-scald medicine currently on the market Type is various, but generally existing cannot relieve pain rapidly, cure low, the course for the treatment of is long, it is inconvenient to use, more after there are apparent scars etc. Problem.
Invention content
The technical problem to be solved by the present invention is to provide one kind capable of relieving pain rapidly treatment burn and scald, cure rate is high, the course for the treatment of It is short, easy to use, to once or two degree of burn and scalds more after there are the topical compositions of scar.
The object of the present invention is to provide a kind of topical compositions for treating burn and scald.
Another object of the present invention is to provide a kind of preparation methods of topical composition that treating burn and scald.
The present invention is achieved through the following technical solutions：
The bulk pharmaceutical chemicals group of the present composition becomes：
The bulk pharmaceutical chemicals of the present composition form：
The bulk pharmaceutical chemicals of the present composition form：
The bulk pharmaceutical chemicals of the present composition form：
The bulk pharmaceutical chemicals of the present composition form：
The preparation method of the present composition is：By the root bark of white mulberry, hawksbill turtle and Semen Cuscutae, ultramicro grinding obtains micropowders respectively, 85% or more grain size of micropowder is no more than 6 microns after ultramicro grinding；Rough melic herb and daghestan sweetclover herb are cleaned, the 3-9 times of water measured is added It impregnates 20-60 to divide hour, the complex enzyme of 20-60 parts by weight is added, the amylase and pectase that the complex enzyme is 3: 1 are advanced 30-50 DEG C of 20-40 minutes enzymolysis of row, is then decocted, and is decocted 0.5-1.5 hours, is filtered to get filtrate for the first time；The dregs of a decoction are again It being decocted 0.3-0.7 hours with the 1-3 times of water measured, filtering obtains filtrate, merges filtrate twice, filtering, and filtrate centrifuges, 3000rpm is centrifuged, and is centrifuged 3-9 minutes, is detached supernatant, is obtained supernatant A；The water logging that cicada fungus and Folium Apocyni Veneti are measured with 2-6 times Bubble 20-40 minutes, is added the complex enzyme of 15-45 parts by weight, and the cellulase and amylase that the complex enzyme is 4: 1 first carry out 30-50 DEG C of 20-40 minutes enzymolysis, is then decocted, and is decocted 0.5-1.5 hours for the first time, and filtering obtains filtrate, the dregs of a decoction are again It is decocted 0.3-0.7 hours, is filtered to get filtrate with the 1-3 times of water measured, merge filtrate twice, filtration, filtrate is concentrated into 60-100 DEG C Survey relative density is 1.15-1.18, adds ethyl alcohol to make alcohol content up to 65%, stands, takes supernatant to recycle ethyl alcohol, obtain supernatant B；It will Ultramicro-powder is placed on CO by remaining rhizoma arisaematis, corydalis tuber, kaladana, frankincense, myrrh and centella ultramicro grinding to 30-50 mesh2Extraction It takes in kettle and impregnates 1-3 hours, it is 20-30MPa to control pressure in extraction kettle；Ultramicro-powder after immersion is divided in separating still From it is 4-8MPa to control separating pressure value in separating still, is heated up to 30-50 DEG C, is kept for 3-7 hours, CO2Flow maintain 20-40Kg/h；Extract C is obtained after separation；Supernatant A, supernatant B and extract C are mixed evenly, Sang Bai is added Skin, hawksbill turtle and Semen Cuscutae micropowders are stirred for uniformly being eventually adding the benzene of the glycerine and 10-30 parts by weight of 50-150 parts by weight Sodium formate is mixed evenly, and externally-applied soft ointment is made.
The preparation method of the present composition is：By the root bark of white mulberry, hawksbill turtle and Semen Cuscutae, ultramicro grinding obtains micropowders respectively, 85% or more grain size of micropowder is no more than 6 microns after ultramicro grinding；Rough melic herb and daghestan sweetclover herb are cleaned, the water logging of 6 times of amounts is added 40 points of hours are steeped, the complex enzyme of 40 parts by weight is added, the amylase and pectase that the complex enzyme is 3: 1 first carry out 40 DEG C 30 The enzymolysis of minute, is then decocted, and is decocted 1 hour, is filtered to get filtrate for the first time；It is small that the water that the dregs of a decoction are measured with 2 times again decocts 0.5 When, filtering obtains filtrate, merges filtrate twice, filtering, and filtrate centrifuges, and 3000rpm centrifugations centrifuge 6 minutes, detach supernatant Liquid obtains supernatant A；The water that cicada fungus and Folium Apocyni Veneti are measured with 4 times is impregnated 30 minutes, the complex enzyme of 30 parts by weight is added, it is described The cellulase and amylase that complex enzyme is 4: 1 first carry out 40 DEG C of 30 minutes enzymolysis, are then decocted, and decoct 1 for the first time Hour, filtering obtains filtrate, and the water that the dregs of a decoction are measured with 2 times again is decocted 0.5 hour, filtered to get filtrate, and merges filtrate twice, is filtered, filter It is 1.15-1.18 that liquid, which is concentrated into 80 DEG C and surveys relative density, adds ethyl alcohol to make alcohol content up to 65%, stands, supernatant is taken to recycle ethyl alcohol, Obtain supernatant B；By remaining rhizoma arisaematis, corydalis tuber, kaladana, frankincense, myrrh and centella ultramicro grinding to 40 mesh, by Ultramicro-powder It is placed on CO2It is impregnated 2 hours in extraction kettle, it is 25MPa to control pressure in extraction kettle；By the Ultramicro-powder after immersion in separating still into Row separation, it is 6MPa to control separating pressure value in separating still, is heated up to 40 DEG C, is kept for 5 hours, CO2Flow maintain 30Kg/ h；Extract C is obtained after separation；Supernatant A, supernatant B and extract C are mixed evenly, add the root bark of white mulberry, hawksbill turtle and Semen Cuscutae micropowders are stirred for uniformly being eventually adding the glycerine of 100 parts by weight and the sodium benzoate of 20 parts by weight is mixed Uniformly, externally-applied soft ointment is made.
The present composition according to common process be added customary adjuvant be made the externally-applied soft ointment of clinical receiving, spray, Liniment, powder, drops or tincture.
Bulk pharmaceutical chemicals in the present composition carry out compatibility, wherein rough melic herb, daghestan sweetclover herb, the root bark of white mulberry, cicada by monarch Flower, hawksbill turtle, Folium Apocyni Veneti, 7 taste bulk pharmaceutical chemicals of Semen Cuscutae are monarch drug in a prescription, play main treatment in the present invention treats burn and scald and make With another rhizoma arisaematis, corydalis tuber and 3 taste bulk pharmaceutical chemicals of kaladana are ministerial drug, and monarch drug in a prescription is assisted to reinforce treatment burn and scald in the present invention Adjuvant, other frankincenses, myrrh and 3 taste bulk pharmaceutical chemicals of centella are adjutant and make medicine, play the work of accompanying symptoms in the compositions of the present invention With.The core of the present composition is the proportion relation between bulk pharmaceutical chemicals, and identical bulk pharmaceutical chemicals carry out different in the composition Distribution and proportioning, generated effect are also completely different.Chinese medicine composition it is most exquisite be each bulk pharmaceutical chemicals proportioning association Same-action, the interpromoting relation in five elements of some bulk pharmaceutical chemicals groups credit union, the restriction or checking relation in five elements of some bulk pharmaceutical chemicals groups credit union, the combination of different bulk pharmaceutical chemicals, different proportionings, Different therapeutic effects can be brought, and some can also bring toxic side effect.
Inventor has found in screening process, and the root bark of white mulberry, hawksbill turtle and Semen Cuscutae ultramicro grinding are only obtained micropowders, will Rough melic herb and daghestan sweetclover herb centrifuge to obtain supernatant A after being decocted with 3: 1 amylase and pectase；By cicada fungus and Folium Apocyni Veneti with 4: 1 Cellulase and amylase decoct recycling ethyl alcohol obtain supernatant B；Remaining 6 taste bulk pharmaceutical chemicals is detached after ultramicro grinding CO2It is impregnated 2 hours in extraction kettle, it is 25MPa to control pressure in extraction kettle；Ultramicro-powder after immersion is divided in separating still From, extract C is obtained, the form decocted by these methods is used as medicine, and obtained yield highest, the effect that ointment is made is best, Effective ingredient will not mutually be destroyed so that effective ingredient is more stable.Due to and not all bulk pharmaceutical chemicals be suitable for being carried with complex enzyme Take, be not yet suitable multiplex-enzyme extraction the medicinal arbitrary multiplex-enzyme extraction effect of raw material it is all good, different bulk pharmaceutical chemicals according to The different selected complex enzymes of its composition are also not upper identical.If all using multiplex-enzyme extraction, yield to improve not all bulk pharmaceutical chemicals Obviously, and composition is also unstable, can also greatly increase production this at,
Using Chinese medical extract as the externally-applied soft ointment of chief component, due to more than extract component and complicated oneself viscosity Higher, general externally-applied soft ointment absorption is slower, influences its treatment results.Therefore, the present invention is added to glycerine and sodium benzoate After made externally-applied soft ointment, it just can be good at discharging drug after 10 minutes, and ointment compares lubrication, will not dry, Best therapeutic effect can be reached.
The root bark of white mulberry, hawksbill turtle and Semen Cuscutae are used into ultramicro grinding in the preparation method of the present composition, grain size of micropowder is small, Since ultramicro grinding process materials temperature is less than -18 DEG C；Chinese medicine Ultramicro-powder after ultramicro grinding, it is pollution-free, Chrome-free, nickel, The peeling of the heavy metals break flour particle such as cadmium, titanium, iron, copper, zinc, lead, arsenic disengages.By rough melic herb and daghestan sweetclover herb with 3: 1 amylase and Pectase centrifuges after decocting, and so that recovery rate is greatly improved, active ingredient is not also destroyed, and the effective ingredient for extracting drug is completeer Entirely, the features such as dissolution rate of drug is high, drug effect retention time is long and bioavilability is high.Separately by cicada fungus and Folium Apocyni Veneti with 4: 1 Cellulase and amylase decoct recycling ethyl alcohol extract so that its active ingredient extraction more completely, content higher, stabilization Property it is more preferable, this method purifies extract, and curative effect, there has also been significantly improving, is also greatly reduced with traditional technics comparing Production cost；The improved technique of the present invention also added appropriate amount of auxiliary materials, add made ointment after auxiliary material absorb it is very fast, It just can be good at discharging drug after 10 minutes, reach best therapeutic effect.
Application of the continuous someone's studying enzyme in traditional Chinese medicine extraction in recent years, and achieve good effect.The present invention adopts The advanced technology is optimized and improves to traditional decoction technique according to the property feature of raw material pharmaceutically active ingredient.For enzyme Lot of experiments has been done in the selection of type and dosage.Finally obtain ointment made from above-mentioned preparation method and the method.The party Method purifies extract, and in the case where reducing a large amount of impurity, obtained extract improves significantly than conventional method, And active ingredient also stablize by more former traditional handicraft, curative effect is more also significantly increased with traditional handicraft, is also greatly reduced life Produce cost.Different bulk pharmaceutical chemicals, the enzyme and dosage being applicable in are not quite similar.The effect digested when the dosage of enzyme is relatively low is poor, It increases with the concentration of enzyme, increases with the contact area of substrate, enzyme digestion reaction rate increases, but not the higher the better, when enzyme reaches It then will produce inhibiting effect when to a certain concentration, enzyme is not fully utilized, and causes to waste.Therefore, type of the inventor to enzyme And dosage is investigated, it is found that difference on effect is apparent.The multiplex-enzyme extraction gold wherein combined with 3: 1 amylase and pectase The best results of silk grass and daghestan sweetclover herb, the multiplex-enzyme extraction cicada fungus combined with 4: 1 cellulase and amylase and Folium Apocyni Veneti Best results, make the extraction of these raw material the effective elements of the medicines more completely, content higher, stability it is more preferable, this method is to extraction Object is purified, and there has also been significantly improve with traditional technics comparing for curative effect.
It is an advantage of the current invention that bulk pharmaceutical chemicals compatibility selected by this external used medicine is suitable, meet traditional Chinese medicine and pharmacy and modern medicine Theory, and with heat-clearing toxin expelling, anti-inflammatory analgetic, removing necrosis, promoting granulation, anti-infective, promote wound healing fast, preventing from scar after healing, Therapeutic effect is notable.The present invention is by carrying out optimal distribution and proportioning to each bulk pharmaceutical chemicals so that each bulk pharmaceutical chemicals are in the present invention In reach best therapeutic effect, and mutually auxiliary phase assistant can be played the role of simultaneously.
Rough melic herb alias falls Soviet Union, Herba pogonatheri criniti, Mao Maocao, crinite pogonatherum herb, kitten grass, the top of a wall bamboo, Latin in the present composition Literary fame Pogonatherum crinitum (Thunb.) Kunth., rough melic herb category, grass family, Monocotyledonae, angiosperm Door.Rough melic herb has heat-clearing, relieving summer-heat, diuresis.For high fever of catching a cold, heatstroke, urinary tract infections, nephritic dropsy, icteric hepatitis, sugar Urine disease, children long do not move back by heat, clearing heat and detoxicating, cooling blood and hemostasis, dampness removing.Main pyreticosis polydipsia；It spits blood；Bleeding from five sense organs or subcutaneous tissue；Hemoptysis；Hematuria；Metrorrhagia； Jaundice；Oedema；Under stranguria with turbid discharge band, rush down dysentery；Children's infantile malnutrition due to digestive disturbances or intestinalparasites heat；Furunculosis carbuncle swells.Daghestan sweetclover herb is also known as wild alfalfa, Pin Chuan bush clover, Fu bush clover etc..2 years Raw or annual herb, it is 60~120 centimetres high.Stem is upright, multi-branched.Daghestan sweetclover herb has heat-clearing, removing toxic substances, dampness elimination, desinsection.Control heat Hot uncomfortable in chest, malaria, dysentery, gonorrhoea, skin ulcer.The root bark of white mulberry is moraceae plants mulberry (Classification system：Morus alba L.) it is dry Dry root skin.Alias Cortex Mori, mulberry root skin, mulberry skin, Bai Sangpi.The root bark of white mulberry has decompression, diuresis, calmness, analgesia, anti-inflammatory and anti- The effects that bacterium.Cicada fungus (Isaria cicadae Miquel) is commonly called as big cordyceps sinensis, belongs to entomogenous fungi；In the whole world it has been found that 504 kinds of cordyceps sinensis in, golden cicada flower, cordyceps sinensis be only two kinds therein.Cicada fungus work sweet in flavor, cold in nature, with nourishing liver and improving eyesight With suitable for muscae volitantes, cataract, yctalopia, eye-blurred etc..Hawksbill turtle (scientific name：Eretmochelys imbricata) belong to Reptilia, the marine animal of Cheloniidae.Hawksbill turtle has the function of clearing heat and detoxicating, relieving palpitation and soothing the liver.Folium Apocyni Veneti, alias tealeaves Flower, wartwort fiber crops, wild tealeaves, sage, wild flax；Latin literary fame：Dogbane leaf. Angiospermaes, Dicotyledoneae, sieve The leaf of cloth fiber crops, soothing the liver are calmed the nerves, clearing away heat and promoting diuresis.For dizziness due to hyperactivity of liver-YANG, palpitation and insomnia, edema oliguria；Hypertension, neurasthenia, Ephritis edema.Semen Cuscutae (scientific name：Cuscuta chinensis) also known as SEMEN CUSCUTAE, without ma rattan, dodder laurel, trailing plants silk, be japanese bearbind One kind under section's semen cuscatue spp, be a kind of special parasitic plant of physiological make-up, does not have chloroplaset in the cell of composition, profit It is sought connections in other plant with rattan columnar structure is climbed, and spine is stretched out from the position of contact host, protruded into the through bast of host, inhale Nutrient is taken to tie up life, further can also store into amylum body in tissue.Semen Cuscutae is the good medicine of flat benefit kidney,liver,spleen simply, Clinic is mainly used in the kidney yin yang deficiency syndrome of the dark loose stool of lumbago due to the kidney deficiency, impotence and seminal emission, frequent micturition, uterus cold infertile, mesh.Rhizoma arisaematis (English Literary fame：RHIZOMA ARISAEMATIS) herbal medicine included of Chinese Pharmacopoeia is plant Araeceae, rhizoma arisaematis (Arisaema Erubescens (Wall.) Schott.), Arisaema heterophyllum Blume (Arisaema heterophyllum Blume) or northeast Tian Nan The dry tuber of star (Arisaema amurense Maxim.).Rhizoma arisaematis has the function of expelling wind and relieving convulsion, reducing phlegm and resolving masses.Main apoplexy Phlegm heap soil or fertilizer over and around the roots, facial paralysis, hemiplegia, paralysis of the limbs, anemophlegmatic vertigo, epilepsy, infantile convulsion, lockjaw, cough ant phlegm, carbuncle swells, scrofula, Bruise paralysis, venomous snake bite.Corydalis tuber is the dry tuber of papaveraceae plant corydalis.Early summer cauline leaf is excavated when withered, is removed Fibrous root is cleaned, and is set when boiled in boiling water to proper without the white heart, is taken out, is dried.Has effects that analgesic.For chest side of body, epigastric pain, Chest impediment and cardialgia, closed dysmenorrhea, postpartum stasis, tumbling and swelling.Kaladana's (Classification system：Pharbitis nil (L.) Choisy), Belong to Convolvulaceae ipomoea, annual voluble herb.The flower of this kind of plant exactly like it is horn-like, therefore some place make it make loudspeaker Flower.There is kaladana water pouring defaecation, dissolving phlegm to wash drink, and desinsection attacks product.For oedema turgor, constipation and anuresis, phlegm and retained fluid accumulation, circulation of vital energy in the wrong direction asthma Cough, abdominal pain due to enterositosis, roundworm disease, taeniasis.Frankincense is for olive subject plant Boswellia carterii Boswellia carterii Birdw and together The resin of platymiscium Boswellia bhaurdajiana Birdw barks exudation.There is frankincense regulating qi and activating blood, analgesic therapy to chase after poison. Control qi and blood stagnation, trusted subordinate's pain, ulcerative carbuncle pyogenic infections, traumatic injury, dysmenorrhoea, postpartum blood stasis stabbing pain.Myrrh is olive subject plant myrrh Set CommiphoramyrrhaEngl. or the gum resin of Alan fort myrrh.Myrrh cures mainly chest and abdomen stasis caused pain, dysmenorrhoea, Amenorrhea, wei Lump in the abdomen, traumatic injury, carbuncle swells sore, acute appendicitis, red eye, swell pain.There are promoting blood circulation and stopping pain, detumescence and promoting granulation and other effects.Drug for invigorating blood circulation and eliminating stasis.Accumulated snow Careless (scientific name：Centella asiatica (L.) Urban) perennial herb, stem is crawled, elongated, is taken root on section.Centella is clear Hot dampness removing, subdhing swelling and detoxicating, control acute diseases such as cholera and sunstroke xenon abdominal pain, heat rushes down, dysentery, jaundice with damp-heat pathogen, sand leaching, blood strangury, haematemesiss, hemoptysis, hot eyes, larynx swell, Rubella, mange, malignant boil carbuncle swells poison, traumatic injury etc..The all medicines of the present invention merge with heat-clearing toxin expelling, anti-inflammatory analgetic, removing necrosis, promoting granulation, resist Infection dynamics is strong, promotes wound healing fast, preventing from scar after healing, and therapeutic effect is notable.
Experimental example is for further illustrating the present invention below, but is not limited to the present invention.
The screening test of test example one, bulk pharmaceutical chemicals proportioning
1. materials and methods
1.1 experiment materials are shown in Table 1
1 unit of table：g
1.2 preparation method
Prescription 1- prescriptions 6 are prepared by the following method in above-mentioned table 1.
By the root bark of white mulberry, hawksbill turtle and Semen Cuscutae, ultramicro grinding obtains micropowders respectively, after ultramicro grinding 85% or more it is micro- Powder diameter is no more than 6 microns；Rough melic herb and daghestan sweetclover herb are cleaned, adds the water of 6 times of amounts to impregnate 40 points of hours, 40 parts by weight is added Complex enzyme, the amylase and pectase that the complex enzyme is 3: 1 first carry out 40 DEG C of 30 minutes enzymolysis, are then decocted, the It is primary to decoct 1 hour, it filters to get filtrate；The water that the dregs of a decoction are measured with 2 times again decocts 0.5 hour, filtering, obtains filtrate, and merging is filtered twice Liquid, filtering, filtrate centrifuge, and 3000rpm centrifugations centrifuge 6 minutes, detach supernatant, obtain supernatant A；By cicada fungus and spread out The water of 4 times of amounts of hemp leaf beetle impregnates 30 minutes, and the complex enzyme of 30 parts by weight is added, the cellulase and shallow lake that the complex enzyme is 4: 1 Powder enzyme first carries out 40 DEG C of 30 minutes enzymolysis, is then decocted, and decocts 1 hour for the first time, and filtering obtains filtrate, the dregs of a decoction are used again The water of 2 times of amounts decocts 0.5 hour, filters to get filtrate, and merges filtrate twice, filtration, and filtrate is concentrated into 80 DEG C of survey relative densities and is 1.15-1.18 adds ethyl alcohol to make alcohol content up to 65%, stands, takes supernatant to recycle ethyl alcohol, obtain supernatant B；By remaining rhizoma arisaematis, Ultramicro-powder is placed on CO by corydalis tuber, kaladana, frankincense, myrrh and centella ultramicro grinding to 40 mesh2It is small that 2 are impregnated in extraction kettle When, it is 25MPa to control pressure in extraction kettle；Ultramicro-powder after immersion is detached in separating still, controls in separating still and divides Tripping force value is 6MPa, is heated up to 40 DEG C, is kept for 5 hours, CO2Flow maintain 30Kg/h；Extract C is obtained after separation；It will Supernatant A, supernatant B and extract C are mixed evenly, and add the root bark of white mulberry, hawksbill turtle and Semen Cuscutae micropowders and are stirred for Uniformly, the sodium benzoate of the glycerine and 20 parts by weight that are eventually adding 100 parts by weight is mixed evenly, and externally-applied soft ointment is made.
1.3 experiment medications
The ointment of prescription 1- prescriptions 6, each 200g are taken respectively.
2, the influence swollen enough to mouse carrageenan
70 male mices, half male and half female, weight (22 ± 2) g are selected in this experiment.It is burnt respectively in the right back foot part of mouse Modeling is scalded, and inflammation is caused 1% lrish moss mucilage 0.05ml is subcutaneously injected.Then it is randomly divided into 7 groups again, blank group and prescription 1- 6 groups of prescription, every group 10.Blank group gives normal saline flushing, and 6 groups of prescription 1- prescriptions are smeared at mouse modeling corresponding respectively Ointment, it is each primary daily morning and evening, it is continuous to smear medicine 7 days.In 30 minutes after the last administration, respectively after causing scorching preceding and cause scorching 1,2,3,4 and 6 hours, diameter at every rat right hind leg ankle-joint lower section 0.5cm is measured with projecting apparatus (8 times of amplification), with cause Swelling difference before scorching with cause different time points after scorching is as swelling.
The influence that table 2 swells enough to mouse carrageenan
By 2 result of above-mentioned table as it can be seen that prescription 1 is to swollen inhibiting effect and the effect for having highly significant enough of mouse carrageenan Time is longer, and anti-inflammatory intensity is most strong.It is substantially better than prescription 2- prescriptions 6.There were significant differences compared with prescription 2- prescriptions 6 for prescription 1； There were significant differences compared with blank group for prescription 1- prescriptions 6.
3. the influence pair the reaction of mouse acetic acid twisting
Select 70 male mices, half male and half female, weight (22 ± 2) g.Burn and scald is carried out in the right back foot part of mouse respectively to make Mould, and the glacial acetic acid solution 0.2ml/ of intraperitoneal injection 0.67% only, 7 groups are then randomly divided into again, at blank group and prescription 1- 6 groups, every group 10 of side.Blank group gives normal saline flushing, and 6 groups of prescription 1- prescriptions are smeared at mouse modeling corresponding respectively Ointment, it is each primary daily morning and evening, it is continuous to smear medicine 7 days.Then each group used counter records the in 40 minutes after the last administration The writhing number of two every mouse in 10 minutes.Compared with the average writhing number of administration each group is averaged writhing number with blank group.See Table 3
The influence that table 3 reacts mouse acetic acid twisting
Grouping Number of animals (only) Average writhing number (X ± SD times)
Blank group 10 35±3
Prescription 1 10 7±1
Prescription 2 10 16±2
Prescription 3 10 18±3
Prescription 4 10 26±4
Prescription 5 10 22±4
Prescription 6 10 21±4
By 3 result of above-mentioned table as it can be seen that prescription 1 can significantly decrease acetic acid induced mice writhing number, and the town of prescription 1 Pain effect is most strong.It is substantially better than prescription 2- prescriptions 6.There were significant differences compared with prescription 2- prescriptions 6 for prescription 1；Prescription 1- prescriptions 6 with Blank group compares that there were significant differences.
4. the swollen influence experiment of pair mouse knoting oil ear
70 mouse, half male and half female, weight (22 ± 2) g are selected in experiment.It is randomly divided into 7 groups, blank group and prescription 1- prescriptions 6 groups, every group 10.Blank group gives normal saline flushing, and 6 groups of prescription 1- prescriptions are smeared at mouse right ear corresponding soft respectively Cream smears primary, continuous coating 7 days sooner or later daily.In after the last administration, by 2% croton oil proinflammatory agent 0.05ml drops in every Mouse right ear shell causes scorching.It causes inflammation to take off neck after 2 hours and puts to death mouse, cut left and right ear, same portion is laid with diameter 9mm card punch Position auricle, weighs, using the difference of two ear weight as mouse dropsy of ear degree.
The influence that table 4 swells to mouse knoting oil ear
Grouping Number of animals (only) Ear swells degree (X ± SDmg)
Blank group 10 14.1±2.1
Prescription 1 10 5.5±1.2
Prescription 2 10 8.7±1.4
Prescription 3 10 8.9±1.5
Prescription 4 10 12.9±1.6
Prescription 5 10 9.7±1.5
Prescription 6 10 9.4±1.5
By above-mentioned table 4 the results show that prescription 1 is to mice ear the inhibiting effect of highly significant, hence it is evident that better than place Square 2- prescriptions 6.There were significant differences compared with prescription 2- prescriptions 6 for prescription 1；There were significant differences compared with blank group for prescription 1- prescriptions 6.
5. the influence of incrustation and scar more afterwards of pair mouse burn and scald
Select 70 male mices, half male and half female, weight (22 ± 2) g.Burn and scald is carried out in the right back foot part of mouse respectively to make Mould, is then randomly divided into 7 groups again, 6 groups of blank group and prescription 1- prescriptions, every group 10.Blank group gives normal saline flushing, place 6 groups of square 1- prescriptions smear corresponding ointment at mouse modeling respectively, each primary daily morning and evening, and continuous medicine of smearing is seen after 20 days Examine the incrustation healing state and scar situation at mouse modeling.
Table 5 is to the mouse burn and scald influence of incrustation and scar more afterwards
Grouping Number of animals (only) It forms a scab (%) Scar (%)
Blank group 10 20 85
Prescription 1 10 98 5
Prescription 2 10 65 45
Prescription 3 10 71 50
Prescription 4 10 45 60
Prescription 5 10 58 55
Prescription 6 10 55 53
By watch 5 the results show that prescription 1 is most fast to the healing of mouse burn and scald, and basic preventing from scar after treatment.Prescription It 98% has all formed a scab at modeling after 1 treatment 20 days, only 5% slightly scar, remaining 95% has restored as before, without there are scars Trace.There were significant differences compared with prescription 2- prescriptions 6 for prescription 1.
Experimental example two, clinical trial
1, general information
(1) selection I degree, II degree burned patient totally 400, age 2-65 Sui, wherein male 200, women 200. 8 groups are equally divided into, every group of 50 people are treated according to following therapy respectively, are treated 10 days altogether.
(2) III degree and the above burned patient 160, age 2-65 Sui, wherein male 80, women 80 are selected.It is flat 8 groups are divided into, every group of 20 people are treated according to following therapy respectively, are treated 15 days altogether.
2, drug forms
Of the present invention group 1：Ointment is prepared according to 1 method of embodiment.
Of the present invention group 2：Ointment is prepared according to 2 method of embodiment.
Of the present invention group 3：Ointment is prepared according to 3 method of embodiment.
Control group 1：Number of patent application 201110110125.6 is prepared into powder according to instance method.
Control group 2：Number of patent application 200510130953.0 prepares paste making agent according to claim 2 and 3 methods.
Control group 3：Number of patent application 201410246433.5 is prepared into liniment according to 1 method of embodiment.
Control group 4：Tincture is made according to 1 method of embodiment in number of patent application 201010295931.0.
Control group 5：Powder is made according to 1 method of embodiment in number of patent application 99112563.0.
3, therapy
Of the present invention group 1：Ointment, every 15g, is applied to affected part daily morning and evening, continuous treatment 10 days.
Of the present invention group 2：Ointment, every 15g, is applied to affected part daily morning and evening, continuous treatment 10 days.
Of the present invention group 3：Ointment, every 15g, is applied to affected part daily morning and evening, continuous treatment 10 days.
Control group 1：Powder changes a medicine, spreads on affected part every three days, continuous treatment 10 days.
Control group 2：Paste changes a medicine, paste is applied on gauze, affected part is spread on every three days, continuous treatment 10 days.
Control group 3：Liniment is wiped daily morning and evening in affected part, continuous treatment 10 days.
Control group 4：Tincture is applied to affected part 4 times a day, continuous treatment 10 days.
Control group 5：Powder changes a medicine, spreads on affected part every three days, continuous treatment 10 days.
4, diagnostic criteria
I degree (slight)；The gross area 10% (children 5%) below；
II degree is burnt, and epidermis is impaired, local erythema and mild swelling, allergy of keenly feeling；The gross area is in 11%~30% (children It is 6%~15%) between, part exudation has blister, redness, pain.
III degree：The gross area is 31%~50%, and corium deep layer is impaired, light red, and there are red point, hypalgesia, healing in surface After have scar.
IV degree：The gross area is between 51%~80% (children are 26%~40%), and skin holostrome is impaired, no blister and nothing The pain sensation can form eschar.
5, efficacy assessment standard
It cures：Whole body burn and scald symptom disappears, wound healing, no scar.
It improves：Constitutional symptom subsides substantially, and the open sore part does not heal completely.
It is not cured：Whole body and local symptom it is unchanged or aggravate.
6, treatment results are shown in Table 6 and table 7：
6 I degree of table, II degree burned patient treatment results
Table 7III degree and the above burned patient treatment results
The treatment results of above-mentioned table 6 and table 7 can show that of the present invention group 1 is burnt in treatment I degree, II degree, III degree or more The total effective rate for scalding patient is optimal, can reach 100%, cure rate is 90% or more.Of the present invention group 2 and present invention group 3 are controlled The total effective rate 70-80% of I degree, II degree, III degree and the above burned patient is treated, other 5 groups of total effective rates are all in 65-80%. It can be obtained by above-mentioned experiment, of the present invention group 1 of therapeutic effect is substantially better than control group 1- control groups 5, and treatment results have aobvious Write difference.
The screening experiment of experimental example three, bulk pharmaceutical chemicals rough melic herb and daghestan sweetclover herb in enzyme class and dosage
1. bulk pharmaceutical chemicals rough melic herb and daghestan sweetclover herb are in the screening experiment for selecting enzyme class
Enzyme or complex enzyme (enzyme class is shown in Table 8) and rough melic herb and daghestan sweetclover herb (wherein rough melic herb 120g wards off Chinese grass 120g) Match
Than for：Enzyme or complex enzyme 40g, rough melic herb and daghestan sweetclover herb 240g；
Rough melic herb and daghestan sweetclover herb 240g are cleaned, add 6 times amount water impregnate 40 points of hours, be added the enzyme in the table 8 of 40g or Complex enzyme first carries out 40 DEG C of 30 minutes enzymolysis, is then decocted, and decocts 1 hour, filters to get filtrate for the first time；The dregs of a decoction are again The water measured with 2 times decocts 0.5 hour, and filtering obtains filtrate, merges filtrate twice, filtering, and filtrate centrifuges, 3000rpm from The heart centrifuges 6 minutes, detaches supernatant, obtains supernatant；
With in table 89 kinds of enzymes or complex enzyme according to said ratio and extracting method extraction rough melic herb and daghestan sweetclover herb, different enzymes The yield difference of inter-species extraction is apparent, wherein it is the most notable with the compound enzyme effect that amylase and pectase (3: 1) coordinate, with it He is compared the enzyme of type, and there were significant differences for yield raising.Refer to table 8.
The screening test result (rough melic herb and daghestan sweetclover herb) of 8 enzyme class of table
According to the screening test of 8 enzyme class of table the results show that 7 amylase of group number and pectase (3: 1) are in extraction rough melic herb With the yield highest of daghestan sweetclover herb, there were significant differences compared with other enzymes, therefore complex enzyme preferred starch enzyme+pectase (3: 1).
2, the ratio of amylase and pectase is screened
The ratio screening test result of 9 amylase of table and pectase
According to the ratio screening test of 9 amylase of table and pectase the results show that 1 amylase of group number and pectase (3: 1) Ratio in the yield for extracting rough melic herb and when daghestan sweetclover herb obtains be highest, there were significant differences compared with other ratios.Therefore it forms sediment The ratio of powder enzyme and pectase preferably 3: 1.
3, complex enzyme (amylase and pectase) is used to extract the dosage screening of rough melic herb and daghestan sweetclover herb
10 complex enzyme of table (amylase and pectase) is used to extract the dosage screening test result of rough melic herb and daghestan sweetclover herb
It is obtained according to 10 test result of table, when complex enzyme selects high dose, the yield of bulk pharmaceutical chemicals rough melic herb and daghestan sweetclover herb is not Only decline without improving to also have, complex enzyme yield of bulk pharmaceutical chemicals rough melic herb and daghestan sweetclover herb in low dosage is decreased obviously, only selects The yield of bulk pharmaceutical chemicals rough melic herb and daghestan sweetclover herb is highest when selecting moderate amount (i.e. in 40g).Therefore it is not the agent height of complex enzyme Higher, the extraction yield of bulk pharmaceutical chemicals is just high, and only compound enzyme amount extracts yield highest in 40g.Therefore the amount of complex enzyme is preferred For 40g.
The screening experiment of experimental example four, bulk pharmaceutical chemicals cicada fungus and Folium Apocyni Veneti in enzyme class and dosage
1. bulk pharmaceutical chemicals cicada fungus and Folium Apocyni Veneti are in the screening experiment for selecting enzyme class
Enzyme or complex enzyme (enzyme class is shown in Table 11) and cicada fungus and Folium Apocyni Veneti (wherein cicada fungus 100g, Folium Apocyni Veneti 100g) Proportioning is：Enzyme or complex enzyme 30g, cicada fungus and Folium Apocyni Veneti 200g；
The water that cicada fungus and Folium Apocyni Veneti are measured with 4 times impregnates 30 minutes, and the enzyme in table 11 or complex enzyme 30g is added, described The cellulase and amylase that complex enzyme is 4: 1 first carry out 40 DEG C of 30 minutes enzymolysis, are then decocted, and decoct 1 for the first time Hour, filtering obtains filtrate, and the water that the dregs of a decoction are measured with 2 times again is decocted 0.5 hour, filtered to get filtrate, and merges filtrate twice, is filtered, filter It is 1.15-1.18 that liquid, which is concentrated into 80 DEG C and surveys relative density, adds ethyl alcohol to make alcohol content up to 65%, stands, supernatant is taken to recycle ethyl alcohol, Obtain supernatant；
With in table 11 9 kinds of enzymes or complex enzyme according to said ratio and extracting method extraction cicada fungus and Folium Apocyni Veneti, it is different The yield difference extracted between enzyme is apparent, wherein it is the most notable with the compound enzyme effect that cellulase and amylase (4: 1) coordinate, It is compared with other kinds of enzyme, there were significant differences for yield raising.Refer to table 11.
The screening test result (cicada fungus and Folium Apocyni Veneti) of 11 enzyme class of table
According to the screening test of 11 enzyme class of table the results show that 7 cellulase of group number and amylase (4: 1) are in extraction cicada fungus With the yield highest of Folium Apocyni Veneti, there were significant differences compared with other enzymes, therefore complex enzyme preferred cellulose enzyme and amylase (3: 1)。
2, the ratio of cellulase and amylase is screened
The ratio screening test result of 12 cellulase of table and amylase
According to the ratio screening test of 12 cellulase of table and amylase the results show that 1 cellulase of group number and amylase The yield that the ratio of (4: 1) is obtained when extracting cicada fungus and Folium Apocyni Veneti is highest, and there were significant differences compared with other ratios.Cause The ratio of this cellulase and amylase preferably 4: 1.
3, complex enzyme (cellulase and amylase) is used to extract the dosage screening of cicada fungus and Folium Apocyni Veneti
13 complex enzyme of table (cellulase and amylase) is used to extract the dosage screening test result of cicada fungus and Folium Apocyni Veneti
It is obtained according to 13 test result of table, when complex enzyme selects high dose, the yield of bulk pharmaceutical chemicals cicada fungus and Folium Apocyni Veneti is not Only decline without improving to also have, complex enzyme yield of bulk pharmaceutical chemicals cicada fungus and Folium Apocyni Veneti in low dosage is decreased obviously, only selects The yield of bulk pharmaceutical chemicals cicada fungus and Folium Apocyni Veneti is highest when selecting moderate amount (i.e. in 30g).Therefore it is not the agent height of complex enzyme Higher, the extraction yield of bulk pharmaceutical chemicals is just high, and only compound enzyme amount extracts yield highest in 30g.Therefore the amount of complex enzyme is preferred For 30g.
Following embodiments can realize the effect of above-mentioned experimental example.
Specific implementation mode
1 ointment of embodiment
By the root bark of white mulberry, hawksbill turtle and Semen Cuscutae, ultramicro grinding obtains micropowders respectively, after ultramicro grinding 85% or more it is micro- Powder diameter is no more than 6 microns；Rough melic herb and daghestan sweetclover herb are cleaned, adds the water of 6 times of amounts to impregnate 40 points of hours, 40 parts by weight is added Complex enzyme, the amylase and pectase that the complex enzyme is 3: 1 first carry out 40 DEG C of 30 minutes enzymolysis, are then decocted, the It is primary to decoct 1 hour, it filters to get filtrate；The water that the dregs of a decoction are measured with 2 times again decocts 0.5 hour, filtering, obtains filtrate, and merging is filtered twice Liquid, filtering, filtrate centrifuge, and 3000rpm centrifugations centrifuge 6 minutes, detach supernatant, obtain supernatant A；By cicada fungus and spread out The water of 4 times of amounts of hemp leaf beetle impregnates 30 minutes, and the complex enzyme of 30 parts by weight is added, the cellulase and shallow lake that the complex enzyme is 4: 1 Powder enzyme first carries out 40 DEG C of 30 minutes enzymolysis, is then decocted, and decocts 1 hour for the first time, and filtering obtains filtrate, the dregs of a decoction are used again The water of 2 times of amounts decocts 0.5 hour, filters to get filtrate, and merges filtrate twice, filtration, and filtrate is concentrated into 80 DEG C of survey relative densities and is 1.15-1.18 adds ethyl alcohol to make alcohol content up to 65%, stands, takes supernatant to recycle ethyl alcohol, obtain supernatant B；By remaining rhizoma arisaematis, Ultramicro-powder is placed on CO by corydalis tuber, kaladana, frankincense, myrrh and centella ultramicro grinding to 40 mesh2It is small that 2 are impregnated in extraction kettle When, it is 25MPa to control pressure in extraction kettle；Ultramicro-powder after immersion is detached in separating still, controls in separating still and divides Tripping force value is 6MPa, is heated up to 40 DEG C, is kept for 5 hours, CO2Flow maintain 30Kg/h；Extract C is obtained after separation；It will Supernatant A, supernatant B and extract C are mixed evenly, and add the root bark of white mulberry, hawksbill turtle and Semen Cuscutae micropowders and are stirred for Uniformly, the sodium benzoate of the glycerine and 20g that are eventually adding 100g is mixed evenly, and externally-applied soft ointment is made.
2 ointment of embodiment
By the root bark of white mulberry, hawksbill turtle and Semen Cuscutae, ultramicro grinding obtains micropowders respectively, after ultramicro grinding 85% or more it is micro- Powder diameter is no more than 6 microns；Rough melic herb and daghestan sweetclover herb are cleaned, adds the water of 6 times of amounts to impregnate 40 points of hours, 40 parts by weight is added Complex enzyme, the amylase and pectase that the complex enzyme is 3: 1 first carry out 40 DEG C of 30 minutes enzymolysis, are then decocted, the It is primary to decoct 1 hour, it filters to get filtrate；The water that the dregs of a decoction are measured with 2 times again decocts 0.5 hour, filtering, obtains filtrate, and merging is filtered twice Liquid, filtering, filtrate centrifuge, and 3000rpm centrifugations centrifuge 6 minutes, detach supernatant, obtain supernatant A；By cicada fungus and spread out The water of 4 times of amounts of hemp leaf beetle impregnates 30 minutes, and the complex enzyme of 30 parts by weight is added, the cellulase and shallow lake that the complex enzyme is 4: 1 Powder enzyme first carries out 40 DEG C of 30 minutes enzymolysis, is then decocted, and decocts 1 hour for the first time, and filtering obtains filtrate, the dregs of a decoction are used again The water of 2 times of amounts decocts 0.5 hour, filters to get filtrate, and merges filtrate twice, filtration, and filtrate is concentrated into 80 DEG C of survey relative densities and is 1.15-1.18 adds ethyl alcohol to make alcohol content up to 65%, stands, takes supernatant to recycle ethyl alcohol, obtain supernatant B；By remaining rhizoma arisaematis, Ultramicro-powder is placed on CO by corydalis tuber, kaladana, frankincense, myrrh and centella ultramicro grinding to 40 mesh2It is small that 2 are impregnated in extraction kettle When, it is 25MPa to control pressure in extraction kettle；Ultramicro-powder after immersion is detached in separating still, controls in separating still and divides Tripping force value is 6MPa, is heated up to 40 DEG C, is kept for 5 hours, CO2Flow maintain 30Kg/h；Extract C is obtained after separation；It will Supernatant A, supernatant B and extract C are mixed evenly, and add the root bark of white mulberry, hawksbill turtle and Semen Cuscutae micropowders and are stirred for Uniformly, the sodium benzoate of the glycerine and 28g that are eventually adding 80g is mixed evenly, and externally-applied soft ointment is made.
3 ointment of embodiment
By the root bark of white mulberry, hawksbill turtle and Semen Cuscutae, ultramicro grinding obtains micropowders respectively, after ultramicro grinding 85% or more it is micro- Powder diameter is no more than 6 microns；Rough melic herb and daghestan sweetclover herb are cleaned, adds the water of 6 times of amounts to impregnate 40 points of hours, 40 parts by weight is added Complex enzyme, the amylase and pectase that the complex enzyme is 3: 1 first carry out 40 DEG C of 30 minutes enzymolysis, are then decocted, the It is primary to decoct 1 hour, it filters to get filtrate；The water that the dregs of a decoction are measured with 2 times again decocts 0.5 hour, filtering, obtains filtrate, and merging is filtered twice Liquid, filtering, filtrate centrifuge, and 3000rpm centrifugations centrifuge 6 minutes, detach supernatant, obtain supernatant A；By cicada fungus and spread out The water of 4 times of amounts of hemp leaf beetle impregnates 30 minutes, and the complex enzyme of 30 parts by weight is added, the cellulase and shallow lake that the complex enzyme is 4: 1 Powder enzyme first carries out 40 DEG C of 30 minutes enzymolysis, is then decocted, and decocts 1 hour for the first time, and filtering obtains filtrate, the dregs of a decoction are used again The water of 2 times of amounts decocts 0.5 hour, filters to get filtrate, and merges filtrate twice, filtration, and filtrate is concentrated into 80 DEG C of survey relative densities and is 1.15-1.18 adds ethyl alcohol to make alcohol content up to 65%, stands, takes supernatant to recycle ethyl alcohol, obtain supernatant B；By remaining rhizoma arisaematis, Ultramicro-powder is placed on CO by corydalis tuber, kaladana, frankincense, myrrh and centella ultramicro grinding to 40 mesh2It is small that 2 are impregnated in extraction kettle When, it is 25MPa to control pressure in extraction kettle；Ultramicro-powder after immersion is detached in separating still, controls in separating still and divides Tripping force value is 6MPa, is heated up to 40 DEG C, is kept for 5 hours, CO2Flow maintain 30Kg/h；Extract C is obtained after separation；It will Supernatant A, supernatant B and extract C are mixed evenly, and add the root bark of white mulberry, hawksbill turtle and Semen Cuscutae micropowders and are stirred for Uniformly, the sodium benzoate of the glycerine and 15g that are eventually adding 120g is mixed evenly, and externally-applied soft ointment is made.
4 spray of embodiment
Customary adjuvant is added according to common process in said medicine, spray is made.It is sprayed on affected part daily morning and evening.
5 tincture of embodiment
Customary adjuvant is added according to common process in said medicine, tincture is made.It is dripped daily morning and evening in affected part.
6 liniment of embodiment
Customary adjuvant is added according to common process in said medicine, liniment is made, wiped daily morning and evening in affected part.

Claims (9)

1. a kind of topical composition for treating burn and scald, which is characterized in that the bulk pharmaceutical chemicals group of the composition becomes：
2. composition as described in claim 1, which is characterized in that the bulk pharmaceutical chemicals group of the composition becomes：
3. composition as claimed in claim 1 or 2, which is characterized in that the bulk pharmaceutical chemicals group of the composition becomes：
4. composition as described in claim 1, which is characterized in that the bulk pharmaceutical chemicals group of the composition becomes：
5. pharmaceutical composition as described in claim 1, which is characterized in that the bulk pharmaceutical chemicals group of the composition becomes：
6. the composition as described in one of claim 1-5, which is characterized in that the preparation method of the composition is：By the root bark of white mulberry, Ultramicro grinding obtains micropowders respectively for hawksbill turtle and Semen Cuscutae, and 85% or more grain size of micropowder is no more than 6 microns after ultramicro grinding； Rough melic herb and daghestan sweetclover herb are cleaned, adds the 3-9 times of water measured to impregnate 20-60 and divides hour, the complex enzyme of 20-60 parts by weight, institute is added Amylase and pectase that complex enzyme is 3: 1 are stated, 30-50 DEG C of 20-40 minutes enzymolysis is first carried out, is then decocted, first Secondary decoction 0.5-1.5 hours, filters to get filtrate；The dregs of a decoction are decocted 0.3-0.7 hours with the 1-3 times of water measured again, and filtering obtains filtrate, Merge filtrate twice, filtering, filtrate centrifuges, and 3000rpm centrifugations centrifuge 3-9 minutes, detach supernatant, obtain supernatant A； Cicada fungus and Folium Apocyni Veneti are impregnated 20-40 minutes with the 2-6 times of water measured, the complex enzyme of 15-45 parts by weight, the complex enzyme is added For 4: 1 cellulase and amylase, 30-50 DEG C of 20-40 minutes enzymolysis is first carried out, is then decocted, decocted for the first time 0.5-1.5 hours, filtering obtained filtrate, and the dregs of a decoction are decocted 0.3-0.7 hours with the 1-3 times of water measured, filtered to get filtrate again, merge two Secondary filtrate, filtration, it is 1.15-1.18 that filtrate, which is concentrated into 60-100 DEG C and surveys relative density, and ethyl alcohol is added to make alcohol content up to 65%, quiet It sets, takes supernatant to recycle ethyl alcohol, obtain supernatant B；Remaining rhizoma arisaematis, corydalis tuber, kaladana, frankincense, myrrh and centella are surpassed Micro mist is broken to 30-50 mesh, and Ultramicro-powder is placed on CO2It is impregnated 1-3 hours in extraction kettle, it is 20- to control pressure in extraction kettle 30MPa；Ultramicro-powder after immersion is detached in separating still, it is 4-8MPa, heating to control separating pressure value in separating still To 30-50 DEG C, kept for 3-7 hours, CO2Flow maintain 20-40Kg/h；Extract C is obtained after separation；By supernatant A, supernatant Liquid B and extract C are mixed evenly, and add the root bark of white mulberry, hawksbill turtle and Semen Cuscutae micropowders and are stirred for uniformly, being eventually adding The glycerine of 50-150 parts by weight and the sodium benzoate of 10-30 parts by weight are mixed evenly, and externally-applied soft ointment is made.
7. composition as claimed in claim 6, which is characterized in that by the root bark of white mulberry, hawksbill turtle and Semen Cuscutae, ultramicro grinding obtains respectively Micropowders, 85% or more grain size of micropowder is no more than 6 microns after ultramicro grinding；Rough melic herb and daghestan sweetclover herb are cleaned, add 6 times The water of amount impregnates 40 points of hours, and the complex enzyme of 40 parts by weight is added, and the amylase and pectase that the complex enzyme is 3: 1 are advanced 40 DEG C of 30 minutes enzymolysis of row, are then decocted, and are decocted 1 hour, are filtered to get filtrate for the first time；The water that the dregs of a decoction are measured with 2 times again It decocting 0.5 hour, filtering obtains filtrate, merges filtrate twice, filtering, and filtrate centrifuges, and 3000rpm centrifugations centrifuge 6 minutes, Supernatant is detached, supernatant A is obtained；The water that cicada fungus and Folium Apocyni Veneti are measured with 4 times is impregnated 30 minutes, the compound of 30 parts by weight is added Enzyme, the cellulase and amylase that the complex enzyme is 4: 1 first carry out 40 DEG C of 30 minutes enzymolysis, are then decocted, first Secondary to decoct 1 hour, filtering obtains filtrate, and the water that the dregs of a decoction are measured with 2 times again is decocted 0.5 hour, filtered to get filtrate, and merges filtrate twice, Filtration, it is 1.15-1.18 that filtrate, which is concentrated into 80 DEG C and surveys relative density, adds ethyl alcohol to make alcohol content up to 65%, stands, supernatant is taken to return Ethyl alcohol is received, supernatant B is obtained；By remaining rhizoma arisaematis, corydalis tuber, kaladana, frankincense, myrrh and centella ultramicro grinding to 40 mesh, Ultramicro-powder is placed on CO2It is impregnated 2 hours in extraction kettle, it is 25MPa to control pressure in extraction kettle；Ultramicro-powder after immersion is being divided From being detached in kettle, it is 6MPa to control separating pressure value in separating still, is heated up to 40 DEG C, is kept for 5 hours, CO2Flow dimension It holds in 30Kg/h；Extract C is obtained after separation；Supernatant A, supernatant B and extract C are mixed evenly, Sang Bai is added Skin, hawksbill turtle and Semen Cuscutae micropowders are stirred for uniformly being eventually adding the sodium benzoate of the glycerine and 20 parts by weight of 100 parts by weight It is mixed evenly, externally-applied soft ointment is made.
8. the pharmaceutical composition as described in one of claim 1-5, which is characterized in that the composition is added normal according to common process Externally-applied soft ointment, spray, liniment, powder, drops or the tincture of clinical receiving is made in rule auxiliary material.
9. application of the composition in the drug for preparing treatment burn and scald as described in one of claim 1-5.
CN201810748150.9A 2018-06-29 2018-06-29 A kind of topical composition and preparation method thereof for treating burn and scald Pending CN108635521A (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1295848A (en) * 1999-11-16 2001-05-23 唐永奎 Powder medicine for treating born and scald
CN102423383A (en) * 2011-04-29 2012-04-25 林大勇 Powder for removing slough and promoting tissue regeneration
CN103977364A (en) * 2014-06-06 2014-08-13 魏放 Traditional Chinese medicine (TCM) preparation for treating burns and scalds
CN108079219A (en) * 2018-02-05 2018-05-29 尹心刚 A kind of topical composition for treating burn and scald and preparation method thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1295848A (en) * 1999-11-16 2001-05-23 唐永奎 Powder medicine for treating born and scald
CN102423383A (en) * 2011-04-29 2012-04-25 林大勇 Powder for removing slough and promoting tissue regeneration
CN103977364A (en) * 2014-06-06 2014-08-13 魏放 Traditional Chinese medicine (TCM) preparation for treating burns and scalds
CN108079219A (en) * 2018-02-05 2018-05-29 尹心刚 A kind of topical composition for treating burn and scald and preparation method thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
刘家磊等: "溃疡速愈散治疗体表急慢性溃疡123例", 《菏泽医学专科学校学报》 *
刘耀亮等: "脱痂一号对兔Ⅲ度烧伤的脱痂作用", 《上海第二医科大学学报》 *
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