CN108633881A - A kind of composition, preparation and its application improving Trifolium repense drought resistance - Google Patents
A kind of composition, preparation and its application improving Trifolium repense drought resistance Download PDFInfo
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- CN108633881A CN108633881A CN201810371295.1A CN201810371295A CN108633881A CN 108633881 A CN108633881 A CN 108633881A CN 201810371295 A CN201810371295 A CN 201810371295A CN 108633881 A CN108633881 A CN 108633881A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/04—Nitrogen directly attached to aliphatic or cycloaliphatic carbon atoms
Abstract
The invention discloses a kind of composition, preparation and its applications improving Trifolium repense drought resistance.Composition includes following parts by weight component：103 parts 619 parts of gamma aminobutyric acid, 8 parts 102 parts of spermine, composite preparation is prepared by composition and acceptable solvent, auxiliary agent, carrier in Pesticide Science.Composition and composite preparation provided by the invention can have the function that synergy by the interaction that intersects of the component mechanism of action, and the increment and yield of Trifolium repense are improved on the basis of improving Trifolium repense drought resistance.
Description
Technical field
The present invention relates to forage cultivating field, specially a kind of composition improving Trifolium repense drought resistance, preparation and its answer
With.
Background technology
As global extreme climate takes place frequently, arid, high temperature etc., which have become, to be influenced crop normal growth, reduces crop yield
The most important abiotic stress factor.Since the 21th century, China crops arid disaster area increases year by year, with
This is simultaneously as China the North and South Available water resources are unevenly distributed, and most of northern China area crops are by arid shadow
It is more acute to ring situation, and southern seasonal extreme drought is also more shown especially.With national Available water resources reduction and
With being continuously increased for water cost, the problem of Crop Drought Resistance is as agriculturist's urgent need to resolve how is improved.
In currently available technology, improve drought resistance single substance be mostly for fruit tree, vegetables, wheat plant growth
Conditioning agent, such as abscisic acid (ABA), methyl jasmonate (MJ), brassinosteroid (BR), TrinexAN_SNacethyl (TE), paclobutrazol
(PP333), cycocel (CCC) etc., but these growth regulators are mostly plant growth suppressive conditioning agent, it is anti-improving crop
Crop yield is caused to decline while inverse property.Secondly small part is deposited for improving the composition of plant drought resistance in the prior art
Mutually act synergistically unobvious between component, cannot connect each other, be mutually converted so as to cause effect in the mechanism of action of component
It is inefficient, crop yield can not be improved while improving drought resistance.
Invention content
The present invention provides a kind of composition, preparation and its application improving Trifolium repense drought resistance.Composition can pass through component
The interaction that intersects of the mechanism of action, has the function that synergy, is improved on the basis of improving Trifolium repense drought resistance
The increment and yield of Trifolium repense.
To solve the above problems, the present invention provides a kind of composition improving Trifolium repense drought resistance, the composition includes
Following parts by weight component：103 parts -619 parts of γ-aminobutyric acid, 8 parts -102 parts of spermine.
Preferably, the composition includes following parts by weight component：103 parts -413 parts of γ-aminobutyric acid, spermine 12
- 31 parts of part.
Preferably, the composition includes following parts by weight component：206 parts of γ-aminobutyric acid, 20 parts of spermine.
Preferably, the application concentration of the composition is γ-aminobutyric acid (1-6) mmol/L, spermine (0.04-05)
mmol/L.Method of administration is root outer spraying.
The present invention also provides preparation, institutes made of acceptable solvent in a kind of composition and Pesticide Science, auxiliary agent, carrier
It is 50-80% to state composition weight and account for the percentage of the total formulation weight.
The dosage form of the preferred preparation is any one in pulvis, aqua, aqueous solution, aqueous emulsion.
Preferably, the solvent in water, ethyl alcohol, ethylene glycol, glycerine, ethanol amine, tetrahydrofurfuryl alcohol any one.
Preferably, the auxiliary agent is selected from alkyl phenol polyoxyethylene ether phosphate, sodium lignin sulfonate, detergent alkylate sulphur
Any one in sour calcium, succinic acid Diisooctyl sodium sulfonate.
Preferably, the carrier in diatomite, kaolin, clay, cornstarch any one.
More preferably the present invention also provides the compositions and preparation to act synergistically, and improves Trifolium repense drought resistance
On application.
Spermine (Spm) is the polyamines of amino and two imino groups containing there are two, as normal presence in organism
Natural active matter and the plant growth regulators that are widely studied, belong to plant parhormone, with very strong life
The drought resistance of plant then can be improved in reason activity, exogenous spermine, improves water use efficiency (WUE) to can also increase biomass.
γ-aminobutyric acid (GABA) is a kind of non-protein composition amino acid, is widely present in animal and plant body.Its
It is not only a kind of important amino acid, and participates in a variety of metabolic activities of plant, is played when plant copes with environment stress crucial
Physiological protection sexual function.External source GABA can remove chloroplaset hydroxy radical under environment stress, stablize and protect chloroplaset
Thylakoid membrane；Metabolism, storage and transhipment that nitrogen source participates in nitrogen are can be used as, maintains plant normal growth, and external source GABA can
It is directly absorbed by root system of plant, by participating in active oxygen metabolism in plant, enhances photosynthesis characteristics, improve plant products.
When spermine and the combination of GABA external sources increase, other than respectively playing above-mentioned effect, also pass through the intersection of mechanism and phase
Interaction reaches synergistic effect.GABA itself participates in spermine degradation pathway in plant, is one of its catabolite, more
Amine degradation generates the paths GABA：Glutamic acid (L-Glutamic acid, L-Glu) generates ornithine by transaminase
(Ornithine, Orn) and arginine (Arginine, Arg), ornithine and arginine pass through ornithine oxidizing ferment
The oxidation of (Ornithinedecarboxylase, ODC) and arginine (Argininedecarboxylase, ADC) generates
Putrescine (Putrescine, put), subsequent putrescine are catalytically converted into spermidine (Spermidine, Spd) through spermidine synzyme,
Spermidine is further converted to spermine (spermine, Spm).Putrescine and spermidine are respectively through diamine oxidase (Diamine
Oxidase, DAO, EC 1.4.3.6) and polyamine oxidase (Polyamine oxidase, PAO, EC 1.5.3.3) oxidation generation
4- aminobutyl aldehydes are finally turned by aminobutyl aldehyde dehydrogenase (4- aminobutyraldehyde dehydrogenase, ABALDH)
Turn to GABA.
The GABA that external source increases breaks the degradation balance of spermine, so that the direction that reaction is synthesized to spermine is promoted, makes simultaneously
Key enzyme in spermine route of synthesis, ADC (ornithine decarboxylase) and ODC (arginine decarboxylase) activity significantly improve, polyamines
The activity of oxidizing ferment (polyamine oxidase, PAO) reduces, to promote the synthesis of spermine in plant.Coordinate again outer
Source spermine increases, and in a lower spermine concentration range, that is, obtains the effect that external source increases larger concentration spermine,
Haved the function that one it is synergistic.
Therefore the composition of spermine provided by the invention and GABA can effectively enhance cell activity oxygen when being applied to Trifolium repense
Scavenging activity protects biomembrane function, improves chlorophyll content of plant, improves water lock rate, reduces mda content, raising can
The synthesis of dissolubility albumen to improve the drought resistance of Trifolium repense, while reaching raising increment, improves the purpose of yield.This hair
For the composition component that the technical solution of bright offer is selected compared with similar other products, production cost is low, cheap, suitable
It is widely applied, secondly present composition application effect is influenced not by administration time and using kind, has wide spectrum
Synergistic function.
Specific implementation mode
In order to make those skilled in the art more fully understand the technical solution of invention, With reference to embodiment
The present invention is described in further detail.
Trifolium repense (Trifolium repens L.) is a kind of trial of Eleven Herbage Plants in Henan of pulse family Clover, is originated in
Europe and north African have been used as high quality forage in cultivation extensively all over the world at present.It is each in China middle and lower reach of Yangtze River Plain and southwest
The Hills And Low Mountains on ground, Trifolium repense have become main leguminous forage, and Trifolium repense is also urban landscaping, soil and slope protection, is reduced
The important ecological construction grass seeds of soil erosion, plays a great role in grassland vegetation recovery, soil and slope protection, afforestation.So
And Trifolium repense category shallow root system grass seeds, anti-transpiration ability is weak, and drought resistance is poor compared with other high quality forages such as clover.By irrigation item
The influence of part, shortage of resources of pouring water and irrigation cost, Trifolium repense underproduction caused by arid or the phenomena of mortality are increasingly in production
It is prominent.Therefore, in Trifolium repense cultivation, it is badly in need of seeking the cultivation step and method for improving Trifolium repense drought resistance.
When arid occurs, the plant cell membrane of Trifolium repense can be damaged first, and arid to the injury of cell membrane with
It the raising of stress level and increases, and stress level more high conductivity is bigger.Conductivity is increased the reason is that arid make plant
Object cell serious dehydration, film fat molecular structure get muddled, and in turn result in the damage of plasmalemma, and stability reduces, and permeability increases
Greatly, intracellular electrolyte largely exosmoses, and outer molten ion increases, and relative conductivity increases.Secondly it under drought condition, plants
The content of object chlorophyll increases in a certain range, is gradually reduced with the aggravation of drought stress, it is withered to be eventually exhibited as blade
It is yellow.Again, when plant is by adverse circumstance such as arid or aging, it may occur that the peroxidation of film fat, malonaldehyde (MDA) are then
One of the Peroxidation Product of film fat, therefore under drought stress, biomembrane is impaired, and Lipid peroxidation metabolism is reinforced, and mda content increases,
Its content height can directly reflect the extent of damage of membrane.Finally, in entire stress during drought stress, soluble protein
Presentation first increases the trend reduced afterwards, and the hydrophily of soluble protein is stronger, and the retention ability of cell can be remarkably reinforced in it, thus
The increase of soluble protein can fetter more juicy, and plant is while soluble protein increases under environment stress, component
Also it can change, the content of some albumen is reduced, and the content of other albumen increases (such as SOD and POD), to complete to plant
Self-protection regulation mechanism under object adverse circumstance.Stress initial stage soluble protein content increases, and illustrates that Trifolium repense has one to arid
Fixed adaptability, the increase content decline with stress concentration are then due to protein biosynthesis block or caused by decomposing reinforcement.
The present invention provides a kind of composition improving Trifolium repense drought resistance, and the composition includes following parts by weight array
Point：103 parts -619 parts of γ-aminobutyric acid, 8 parts -102 parts of spermine.Preferably, 103 parts -413 parts of γ-aminobutyric acid, spermine 12
- 31 parts of part.More preferably, 206 parts of γ-aminobutyric acid, 20 parts of spermine.The application concentration of the composition is gamma-amino fourth
Acid (1-6) mmol/L, spermine (0.04-05) mmol/L.Method of administration is root outer spraying.
The present invention also provides preparation, institutes made of acceptable solvent in a kind of composition and Pesticide Science, auxiliary agent, carrier
It is 50-80% to state composition weight and account for the percentage of the total formulation weight.The dosage form of the preferred preparation is pulvis, water
Any one in agent, aqueous solution, aqueous emulsion.Preferably, the solvent be selected from water, ethyl alcohol, ethylene glycol, glycerine, ethanol amine,
Any one in tetrahydrofurfuryl alcohol.Preferably, the auxiliary agent is selected from alkyl phenol polyoxyethylene ether phosphate, sodium lignin sulfonate, ten
Any one in dialkyl benzene sulfonic acids calcium, succinic acid Diisooctyl sodium sulfonate.More preferably, the carrier be selected from diatomite,
Any one in kaolin, clay, cornstarch.
The present invention finally additionally provides above-mentioned composition and preparation and is acting synergistically, and improves answering in Trifolium repense drought resistance
With.
Spermine (Spermine, SPM) in the present composition, chemical name [N, N '-bis- -3- Propylaminos], molecular weight
202.3, colorless needle crystals or white crystalline powder, the easy moisture absorption, soluble easily in water, lower alcohols and chloroform do not dissolve in second
Ether, benzene, petroleum ether, 55-60 DEG C of fusing point, 141-142 DEG C of boiling point (66.66Pa), 230 °F of flash-point (110 DEG C), relative density
d20 4"：0.937.
A certain amount of polyamines can be accumulated when drought stress in plant, polyamines includes putrescine, spermine and spermidine, dry
When non-irrigated stress level is relatively low, a large amount of synthetic polyamines of plant, to enhance the resistance to stress；And prolonging with the drought stress time
Long and stress level increase, plant just no longer synthetic polyamine, but a large amount of synthesizing ethylenes, to be transferred to aging course as early as possible, into
And complete entire life process.Spray the drought resistance that plant can be improved in Exogenous Polyamine, improve water use efficiency (WUE) to
Increase its biomass.
γ-aminobutyric acid (γ-aminobutyric acid, GABA) is a kind of 4 carbon nonprotein amino acid, it in
It was found in the stem tuber of potato for the first time before half a century.In plant, the metabolism of GABA is mainly by GABA branches come complete
At wherein glutamate decarboxylase (enzyme glutamate decarboxylase, GAD), GABA transaminases (GABA
Transaminase, GABA-T) and succinic semialdehyde dehydrogenase (succinic semialdehyde dehydrogenase,
SSADH) it is the key enzyme for participating in this approach.
Endogenic GABA branches may participate in many physiological reactions of plant, intermediate productions of the GABA first as nitrogen metabolism
Object can play the role of storage or transport nitrogen.When the environment stresses such as low temperature, saline and alkaline, hypoxemia and arid inhibit glutamy in crop
Amine synthesis, accelerate protein degradation when, glutamic acid can under the catalysis of GAD Fast back-projection algorithm GABA, therefore, GABA can be used as nitrogen
The interim storage library of element.Next may participate in the signal transduction of plant, is alternatively arranged as Osmolyte regulator and improves work in Response to stress
Object activities of antioxidant enzymes, the accumulation for reducing the substances such as active oxygen (ROS) reduce injury of the environment stress to crop.
External source, which increases GABA, can improve the biomass of crop seedling, improve its photosynthetical system and then make crop accumulation more
More photosynthates；Simultaneously GABA can improve the germination percentage of plant seedlings, germinating energy and sprout under Hypoxia Stress radicle it is long,
Plumular axis is long, alleviates influence of the environment stress to crop, growth and development of plants.Such as under hypoxemia, low temperature, salt stress, external source increases
Plant growth can be promoted by applying GABA, adjust crop Antioxidant Enzyme Systems and chlorophyll photosynthetical system, improve antioxygen in blade and root
Change enzymatic activity, enhance the photosynthetic net assimilation ability of crop, reduces crop activity in vivo oxygen (ROS), H2O2With the content of malonaldehyde,
Alleviate influence of the environment stress to photosynthetic characteristics, to enhance the resistance of reverse of crop.GABA can be in the work of transaminase simultaneously
The amino acid that the adverse circumstances specificity such as heat shock protein and cold temperature induced protein can be synthesized with lower generation, such as asparagine and the third ammonia
Acid induces stress protein in plant to express.
When spermine and GABA external sources increase, other than respectively playing above-mentioned effect, also pass through the intersection of mechanism and phase interaction
With reaching synergistic effect.
In polyamines biosynthesis pathway centered on putrescine substance.The biosynthesis pathway of putrescine mainly has 2：When
Using ornithine as precursor, through ornithine decarboxylase (ornithine decarboxylase, ODC) catalytic decarboxylation, putrescine is generated；
Another acts on lower decarboxylation then using arginine as precursor, in arginine decarboxylase (argininedecarboxylase, ADC),
Again through series of intermediate products such as agmatines, putrescine is indirectly formed.Diamine oxidase (diamine oxidase, DAO) and PAO
It is the key that Put and Spd, Spm degradation in difference catalysis biological body.When external source GABA processing Trifolium repense root systems, ADC and ODC live
Property significantly improve, it is suppressed that the activity of polyamine oxidase (polyamine oxidase, PAO), promote Put, Spd and Spm conjunction
At with accumulation.
Secondly, it is as follows to generate the paths GABA for polyamines degradation：Glutamic acid (L-Glutamic acid, L-Glu) is by turning ammonia
Enzyme generates ornithine (Ornithine, Orn) and arginine (Arginine, Arg), and ornithine and arginine pass through ornithine oxygen
Change enzyme (Ornithinedecarboxylase, ODC) and arginine enzyme (Arginine decarboxylase, ADC) oxygen
Metaplasia is catalytically converted into spermidine at putrescine (Putrescine, put), subsequent putrescine through spermidine synzyme
(Spermidine, Spd), spermidine are further converted to spermine (spermine, Spm).Putrescine and spermidine are respectively through diamines
Oxidizing ferment (Diamine oxidase, DAO, EC 1.4.3.6) and polyamine oxidase (Polyamine oxidase, PAO, EC
1.5.3.3) oxidation generates 4- aminobutyl aldehydes, finally by aminobutyl aldehyde dehydrogenase (4-aminobutyraldehyde
Dehydrogenase, ABALDH) it is converted into GABA.That is catabolites of the GABA as spermine itself, sprays in external source by root
After system is directly absorbed into plant, the degradation balance of spermine can be broken, reaction is made to be promoted to spermine compound direction.Spermine
The mechanism of interaction effect with GABA is as shown in Fig. 1.
Therefore the composition of spermine provided by the invention and GABA has haved the function that synergy when being applied to Trifolium repense,
Cell activity oxygen scavenging capacity can effectively be enhanced, protect biomembrane function, improve chlorophyll content of plant, improve water lock rate, drop
Low mda content improves the synthesis of soluble protein, to improve the drought resistance of Trifolium repense, while reaching raising increment,
Improve the purpose of yield.
Above-mentioned elaborating for the present invention is the embodiment of the present invention below.
Embodiment 1
The preparation method of invention formulation
Preparation method is：
(1) by each raw material in formula according to stoichiometry, input mixing vessel in carry out premixing 10-15 minutes；
(2) material after premixing is subjected to air-flow crushing and obtains pulvis.
Embodiment 2
The preparation method of invention formulation
Preparation method is：
(1) by each raw material in formula according to stoichiometry, input mixing vessel in carry out premixing 10-15 minutes；
(2) material after premixing is subjected to air-flow crushing and obtains pulvis.
Embodiment 3
Preparation method is：
(1) each raw material in formula is added according to stoichiometry and prepares mixed dissolution in kettle；
(2) dissolution time 30-45 minutes；
(3) after dissolving completely, filtering, blowing obtains aqueous solution.
Embodiment 4
Preparation method is：
(1) each raw material in formula is added according to stoichiometry and prepares mixed dissolution in kettle；(2) dissolution time 30-
45 minutes；
(3) after dissolving completely, filtering, blowing obtains aqueous solution.
Embodiment 5
γ-aminobutyric acid 70.0%
Spermine 3.5%
Pure water complements to 100%
Preparation method is：
(1) each raw material in formula is added according to stoichiometry and prepares mixed dissolution in kettle；(2) dissolution time 30-
45 minutes；
(3) after dissolving completely, filtering, blowing obtains aqua.
Embodiment 6
Preparation method is：
(1) spermine is dissolved with tetrahydrofurfuryl alcohol and propylene glycol, forms oil phase；
(2) amino-oligosaccharide is added in the mixed solution of pure water and dissolves, and forms water phase；
(3) water phase addition oil phase is uniformly mixed, obtains aqueous emulsion.
Embodiment 7
Different GABA influence Trifolium repense drought resistance with Spm concentration combinations
(1) test material：Using in production extensive growth condition ' Latin promise ' Trifolium repense is as material to be tested.
(2) Breeding：By sterilized Trifolium repense seed (1g/m2) sow in diameter 24cm, the basin alms bowl of deep 30cm
In, soil is by husky and soil 1 in basin alms bowl：2 ratios mix, and soil mixing homogeneity is good, and each filled soil weight of basin is consistent.
Trifolium repense after planting is put into growth chamber and is germinateed, after germination 7 days each week with 1/2 Hoagland
Pancebrin pours twice, and growth chamber temperature is 23 DEG C of daytime, and at 19 DEG C of night, duration is respectively 12h, and relative humidity is
70%, intensity of illumination is 700 μm of ol (m-2s)-1.The consistent material of growing way is chosen when Trifolium repense was grown to 45 days carries out the arid side of body
Compel processing.
(3) experimental design：The setting of GABA+Spm (mmol/L) concentration combination is 9 following, respectively：0+0 (control), 1+
0.5,1+0.15,1+0.2,2+0.1,2+0.05,2+0.08,4+0.06,6+0.04 amount to 9 processing, used GABA
It is more than 98% with Spm drug purity.GABA and Spm are directly dissolved in distilled water, now with the current, with without GABA's and Spm
Distilled water is handled as a contrast, and often processing setting 6 is independent to be repeated (i.e. each 6 basins of processing).
(4) composition application and drought stress processing：It is irrigated (1L) with corresponding treatment fluid per basin Trifolium repense material, it is right
It directly connects and is irrigated using distilled water.After being disposed, all material stops watering, is positioned in growth chamber and carries out nature
Drought stress.Growth case condition is same as above.
(5) test result
1 difference GABA of table improves Trifolium repense drought resistance effect with Spm concentration combinations
| GABA+Spm(mmol/L) | Blade wilting symptom time of occurrence | Blade is seriously wilted time of occurrence |
| 0+0 | 7th day | 12nd day |
| 1+0.05 | 9th day | 14th day |
| 1+0.15 | 10th day | 15th day |
| 1+0.2 | 10th day | 15th day |
| 2+0.1 | 15th day | 21st day |
| 2+0.05 | 11st day | 17th day |
| 2+0.08 | 11st day | 15th day |
| 4+0.06 | 10th day | 15th day |
| 6+0.04 | 8th day | 14th day |
As can be seen from Table 1, compared with control (0+0), various concentration GABA+Spm compositions can be to a certain extent
Postpone white clover leaves under drought stress to wilt.Wherein, the best composition of effect is 2mmol/L GABA+0.1mmol/L
Spm can postpone Trifolium repense and wilt 20 days or more, and the composition concentration is wilted alleviating white clover leaves, and Trifolium repense drought resisting is improved
Huge advantage is shown in property.
Embodiment 8
Best GABA and influence of the Spm concentration combinations to Trifolium repense drought resistant index
(1) test material：With embodiment 7
(2) Breeding：With embodiment 7
(3) experimental design：According to earlier data, GABA+Spm composition optium concentrations are 2mmol/L+0.1 mmol/L,
This experiment sets 4 processing altogether, and GABA+Spm concentration is respectively：0+0 (control), 2+0,0+0.1,2+0.1 often handle 6 weights
It is multiple.Used GABA and Spm drugs purity is more than 98%.GABA and Spm are directly dissolved in distilled water, now with the current, with
Distilled water without GABA and Spm is handled as a contrast, and often processing setting 6 is independent to be repeated (i.e. each 6 basins of processing).
(4) combined additive application and drought stress processing：With embodiment 7.
(5) observation index：When drought stress 16 days, the relative growth yield of reflection Trifolium repense upgrowth situation is measured respectively；Instead
Reflect the leaf r elative water content of water conservation situation；Reflect the leaf malondialdehyde content of peroxidation of cellular membranes extent of injury；Reacting cells
The turbine blades in electrochemical matter permeability of membrane stability；And intracellular antioxidative defense enzyme superoxide dismutase (SOD), peroxide
Enzyme (POD), catalase (CAT), ascorbic acid peroxidase (APX) activity.
(6) result and analysis
2 best GABA of table and influence of the Spm concentration combinations to Trifolium repense Physiological Indices of Drought Resistance
Note：Per a line (same index), letter is different indicates significant difference (P ＜ 0.05)
From table 2 it can be seen that drought stress makes plant relative growth yield and leaf r elative water content decline, film fat mistake
The accumulation of oxidation product malonaldehyde increases, cell membrane stability is deteriorated (electrolytic leakage increase)；Using single GABA or single
After Spm or GABA+Spm combination preparations, can make under drought stress relative growth yield and leaf r elative water content maintain significantly compared with
High level, while electrolytic leakage and mda content being made to reduce, also make anti-oxidation protection enzymatic activity SOD, CAT, APX
Activity is significantly higher than control, but application GABA+Spm compositions have notable preferable effect than single GABA or Spm；In addition,
Single administration GABA or Spm fail to significantly improve POD enzymatic activitys, but composition GABA+Spm can significantly improve POD enzymatic activitys.
Therefore, the growth of different disposal and physical signs further demonstrate and compare (0+0), single GABA (2+0) and single Spm
(0+0.1) processing compares, and composition 2mmol/L GABA+0.1mmol/L Spm are showed in the drought resistance for improving Trifolium repense
Go out huge advantage.
Embodiment 9
The influence of best GABA and three leaf-making quantity index of Spm concentration combinations dialogue
(1) test material：With embodiment 7
(2) Breeding：Part is cultivated with embodiment 7, materials processing adds blank group, and blank group is dry not do
The plant of non-irrigated Stress treatment
(3) experimental design：According to earlier data, GABA+Spm composition optium concentrations are 2mmol/L+0.1 mmol/L,
This experiment sets 4 processing altogether, and GABA+Spm concentration is respectively：0+0 (control), 2+0,0+0.1,2+0.1 often handle 6 weights
It is multiple.Used GABA and Spm drugs purity is more than 98%.GABA and Spm are directly dissolved in distilled water, now with the current, with
Distilled water without GABA and Spm is handled as a contrast, and often processing setting 6 is independent to be repeated (i.e. each 6 basins of processing).
(4) combined additive is applied：Experimental group is irrigated with every basin Trifolium repense material of blank group with corresponding treatment fluid
(1L), control are directly irrigated using distilled water.After being disposed, all material stops watering, and experimental group is positioned over plant growth
Natural drought stress is carried out in case, growth case condition is same as above.Blank group does not do drought stress processing, ensures that moisture is grown naturally.
(5) observation index：When cultivating 16 days, determination experiment group reflects Trifolium repense upgrowth situation root system with blank group respectively
Length, plant fresh weight.
(6) result and analysis
3 best GABA of table grows Trifolium repense with Spm concentration combinations the influence of yield index
Note：Per a line (same index), letter is different indicates significant difference (P ＜ 0.05)
From table 3 it can be seen that after using single GABA or single Spm or GABA+Spm compositions, drought stress can be improved
Single-strain fresh weight and root length lower and that grow lower plant naturally, but application GABA+Spm compositions than single GABA or
Spm has notable preferable effect；GABA+Spm compositions are wherein applied to drought stress group single-strain fresh weight and root length
Raising degree, already exceed under natural growthing condition not by the single-strain fresh weight and root length of the processed plant of composition,
Achieve the purpose that improve yield while with drought resisting effect.
Embodiment 10
Best GABA and influence of the Spm concentration combinations administration time to application effect
(1) test material：With embodiment 7.
(2) Breeding：With embodiment 7.
(3) experimental design and processing：In the morning 9:00, the morning 11:00, afternoon 14:00, the dusk 17:00, when amounting to 4
Between using 2mmol/L GABA+0.1mmol/L Spm compositions irrigate Trifolium repense.After being disposed, all material stopping is poured
Water is positioned in growth case and carries out natural drought stress, often handles 6 repetitions, growth case condition is the same as example 1.Used GABA
It is more than 98% with Spm drug purity.GABA and Spm are directly dissolved in distilled water, now with the current, with without GABA's and Spm
Distilled water is handled as a contrast, and often processing setting 6 is independent to be repeated (i.e. each 6 basins of processing).
(4) test result
4 best GABA of table is with the Spm composition different administration times to improving Trifolium repense drought resistance effect
| The foliage-spray time | White clover leaves wilting symptom time of occurrence | White clover leaves are seriously wilted time of occurrence |
| The morning 9:00 | 16th day | 21st day |
| The morning 11:00 | 16th day | 21st day |
| Afternoon 14:00 | 16th day | 21st day |
| At dusk 17:00 | 16th day | 21st day |
As seen from Table 4, the different administration time improves Trifolium repense drought resistance effect without apparent to GABA and Spm compositions
It influences.
Embodiment 11
Best GABA is from Spm concentration combinations object to the application effect of different Trifolium repens cultivar drought resistances
(1) test material：Widely applied four kinds of Trifolium repens cultivars in production are selected, are ' Latin promise ', ' sea respectively
Hair ', ' elfin ' and ' Pixie '.
(2) Breeding：With embodiment 7.
(3) experimental design and processing：Made using the optimal combination object 2mmol/L GABA+0.1mmol/L Spm filtered out
Liquid filling, which is poured, for root application pours four kinds of different Trifolium repens cultivars respectively.Used GABA and Spm drugs purity is more than 98%.
GABA and Spm are directly dissolved in distilled water, now with the current, are handled as a contrast with the distilled water without GABA and Spm, often
Processing setting 6 is independent to be repeated (i.e. each 6 basins of processing).
(4) combined additive application and drought stress processing：With embodiment 7.
(5) test result
The processing of 5 exogenous compositions of table improves different cultivars Trifolium repense drought resistance effect
As seen from Table 5, under identical cultivation and drought stress conditions, when not applying composition, ' sea hair ' and ' small essence
The drought resistance of clever ' two kind is higher than ' Latin promise ' and ' Pixie '；But it can be significantly improved using GABA+Spm compositions
The drought resistance of four kinds of different Trifolium repens cultivars illustrates that the composition has broad spectrum activity effect to improving Trifolium repense drought resistance.
It the above is only the preferred embodiment of the present invention, it is noted that above-mentioned preferred embodiment is not construed as pair
The limitation of the present invention, protection scope of the present invention should be subject to claim limited range.For the art
For those of ordinary skill, without departing from the spirit and scope of the present invention, several improvements and modifications can also be made, these
Improvements and modifications also should be regarded as protection scope of the present invention.
Claims (10)
1. a kind of composition improving Trifolium repense drought resistance, which is characterized in that the composition includes following parts by weight component：
103 parts -619 parts of γ-aminobutyric acid, 8 parts -102 parts of spermine.
2. composition according to claim 1, which is characterized in that the composition includes following parts by weight component：γ-
103 parts -413 parts of aminobutyric acid, 12 parts -31 parts of spermine.
3. composition according to claim 2, which is characterized in that the composition includes following parts by weight component：γ-
206 parts of aminobutyric acid, 20 parts of spermine.
4. composition according to claim 1, which is characterized in that the application concentration of the composition is γ-aminobutyric acid
(1-6) mmol/L, spermine (0.04-05) mmol/L.
5. preparation made of acceptable solvent in any one of Claims 1-4 composition and Pesticide Science, auxiliary agent, carrier, described
The percentage that composition weight accounts for the total formulation weight is 50-80%.
6. preparation according to claim 5, which is characterized in that the dosage form of the preparation is pulvis, aqua, aqueous solution, water
Any one in emulsion.
7. preparation according to claim 6, which is characterized in that the solvent is selected from water, ethyl alcohol, ethylene glycol, glycerine, second
Any one in hydramine, tetrahydrofurfuryl alcohol.
8. preparation according to claim 6, which is characterized in that the auxiliary agent be selected from alkyl phenol polyoxyethylene ether phosphate,
Any one in sodium lignin sulfonate, calcium dodecyl benzene sulfonate, succinic acid Diisooctyl sodium sulfonate
9. preparation according to claim 6, which is characterized in that the carrier is selected from diatomite, kaolin, clay, corn
Any one in starch.
10. the preparation described in composition and claim 9 described in claim 4 is acting synergistically, Trifolium repense drought resisting is improved
Application in property.
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| CN114287427A (en) * | 2022-01-06 | 2022-04-08 | 广东省科学院南繁种业研究所 | Composition and preparation for improving drought resistance of cassava and application of composition and preparation |
| CN114651653A (en) * | 2022-02-21 | 2022-06-24 | 西北农林科技大学 | Amino acid application method for improving water utilization rate and fruit quality of apple dwarf rootstock |
| CN114854767A (en) * | 2022-06-01 | 2022-08-05 | 四川农业大学 | Trifolium repens calmodulin-like protein TrCML6 gene and application thereof in drought resistance |
| CN115777707A (en) * | 2023-02-06 | 2023-03-14 | 哈尔滨本晟创新科技有限公司 | Growth regulator and method for improving total phenol content of acanthopanax by matching with spectral illumination |
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| CN114287427A (en) * | 2022-01-06 | 2022-04-08 | 广东省科学院南繁种业研究所 | Composition and preparation for improving drought resistance of cassava and application of composition and preparation |
| CN114287427B (en) * | 2022-01-06 | 2023-04-18 | 广东省科学院南繁种业研究所 | Composition and preparation for improving drought resistance of cassava and application of composition and preparation |
| CN114651653A (en) * | 2022-02-21 | 2022-06-24 | 西北农林科技大学 | Amino acid application method for improving water utilization rate and fruit quality of apple dwarf rootstock |
| CN114651653B (en) * | 2022-02-21 | 2024-01-26 | 西北农林科技大学 | Amino acid application method for improving water utilization rate and fruit quality of apple dwarf stock |
| CN114854767A (en) * | 2022-06-01 | 2022-08-05 | 四川农业大学 | Trifolium repens calmodulin-like protein TrCML6 gene and application thereof in drought resistance |
| CN114854767B (en) * | 2022-06-01 | 2023-07-11 | 四川农业大学 | Trifolium pratense calmodulin-like protein TrCML6 gene and application thereof in drought resistance |
| CN115777707A (en) * | 2023-02-06 | 2023-03-14 | 哈尔滨本晟创新科技有限公司 | Growth regulator and method for improving total phenol content of acanthopanax by matching with spectral illumination |
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