CN114287427B - Composition and preparation for improving drought resistance of cassava and application of composition and preparation - Google Patents
Composition and preparation for improving drought resistance of cassava and application of composition and preparation Download PDFInfo
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Abstract
The invention discloses a composition for improving drought resistance of cassava, a preparation and application thereof, wherein the composition comprises: 0.05mM to 0.3mM beta-aminobutyric acid and 0.05mM to 0.3mM gamma-aminobutyric acid. The composition and the preparation can obviously improve the drought resistance of cassava, and compared with the method of singly using beta-aminobutyric acid or gamma-aminobutyric acid, the compounded application of the composition and the preparation can generate an accumulated beneficial effect on the drought resistance of cassava seedlings, and can be widely applied to crop breeding for improving the drought resistance of cassava.
Description
Technical Field
The invention belongs to the technical field of agricultural preparations, and particularly relates to a composition for improving drought resistance of cassava, a preparation and application thereof.
Background
The productivity of most crops on barren land is limited by environmental stresses including low soil fertility, drought and salinization, and although cassava can survive in poor soil conditions, its yield is limited by extreme environmental conditions. Under the best conditions, the yield per unit of cassava can reach 80 tons per hectare, while the average yield per unit at present is only 12.8 tons. Because of the less investment in growing cassava on marginal land, intensification and expansion of cassava production will depend on its impact on the environment (Okogbenin, setter et al, 2013).
Drought is one of the inevitable and recurring climatic features of the world's climate. Despite our efforts to predict the occurrence of drought and to correct its effects, drought remains one of the most important limiting factors affecting crop growth and productivity worldwide (Chaves et al, 2002). Sufficient water is required for the growth and development of plants. Approximately one third of the land area around the world is arid and semi-arid, whereas periodic climatic drought often occurs in most other land areas. Water shortage can cause plant death and cause huge social problems and economic losses.
On the one hand, as modern science and technology has greatly enhanced our ability to explore natural resources, these natural resources have greatly improved human life. On the other hand, the increasing world population exacerbates water pollution and unpredictable climate changes, further contributing to shortages of water resources (Trenberth et al, 2014). Drought not only causes severe agricultural production losses, but also aggravates ecological damage, land desertification and water and soil loss. Drought stress causes many changes in the morphology and physiological and biochemical levels of all plant organs. Plants have evolved several strategies to cope with drought stress, including escape from drought through short life cycles or developmental plasticity, avoidance of drought through enhanced water uptake or reduced water loss, and improvement of plant drought tolerance through osmotic regulation, antioxidant capacity, and dehydration (Zhang, 2007).
In the prior art, most of single substances for improving drought resistance are plant growth regulators for fruit trees, vegetables and wheat, such as abscisic acid (ABA), methyl Jasmonate (MJ), brassinolide (BR), trinexapac-ethyl (TE), paclobutrazol (PP 333), chlormequat-chloride (CCC) and the like, but most of the growth regulators are plant growth inhibition regulators, which can cause crop yield reduction while improving crop stress resistance. Secondly, in the prior art, the mutual synergistic action of components of a small part of composition for improving the drought resistance of plants is not obvious, and the action efficiency is not high. Therefore, screening a composition capable of remarkably improving the drought resistance of plants is very important.
Disclosure of Invention
Based on this, it is an object of the present invention to provide a composition for improving drought resistance of cassava, which makes the cassava resistant to drought stress.
The specific technical scheme for realizing the aim of the invention comprises the following steps:
a composition for improving drought resistance of cassava, comprising: 0.05mM to 0.3mM of beta-aminobutyric acid and 0.05mM to 0.3mM of gamma-aminobutyric acid.
In some of these embodiments, the composition comprises: 0.05mM to 0.15mM of beta-aminobutyric acid and 0.05mM to 0.15mM of gamma-aminobutyric acid.
In some of these embodiments, the composition comprises: beta-aminobutyric acid in an amount of 0.08mM to 0.12mM and gamma-aminobutyric acid in an amount of 0.08mM to 0.12mM.
The invention also provides a preparation for improving the drought resistance of cassava, and the active ingredients of the preparation comprise the composition.
The invention also provides a preparation method of the preparation for improving the drought resistance of cassava.
The specific technical scheme for realizing the aim of the invention comprises the following steps:
a preparation method of a preparation for improving drought resistance of cassava comprises the following steps: dissolving beta-aminobutyric acid and gamma-aminobutyric acid in water to ensure that the final concentration of the beta-aminobutyric acid and the final concentration of the gamma-aminobutyric acid are both 0.05 mM-0.3 mM.
In some of these embodiments, the final concentration of beta-aminobutyric acid and gamma-aminobutyric acid are each 0.05mM to 0.15mM.
In some of these embodiments, the final concentration of beta-aminobutyric acid and gamma-aminobutyric acid are each 0.08mM to 0.12mM.
The invention also provides application of the preparation for improving the drought resistance of cassava in crop breeding for improving the drought resistance of cassava.
The invention also provides a method for improving the drought resistance of cassava.
A method for improving drought resistance of cassava comprises the following steps: and irrigating the preparation for cassava seedlings.
In some of these embodiments, the cassava seedlings are seedlings that grow in soil for 3 to 8 days.
In some embodiments, the preparation is irrigated for 6-8 days in an amount of 15-25 mL/plant.
Compared with the prior art, the invention has the following beneficial effects:
in the invention, the inventor finds that the survival rate of the cassava seedlings under drought stress is obviously improved by irrigating the cassava seedlings under drought stress with exogenous chemical reagents beta-aminobutyric acid and gamma-aminobutyric acid compounded at certain concentrations, and further researches find that H in the cassava seedlings 2 O 2 The accumulation is reduced, and the activities (including APX, POD and CAT), the expression quantity of genes related to the antioxidase, the transcription level of signal transduction genes related to drought stress and the like of all the antioxidase are obviously improved, so that the preparation disclosed by the invention can obviously improve the drought resistance of cassava, and compared with the situation that beta-aminobutyric acid or gamma-aminobutyric acid is singly used, the combined use generates an accumulated beneficial effect on the drought resistance of cassava seedlings, and the preparation can be widely applied to crop breeding for improving the drought resistance of cassava.
Drawings
FIG. 1 is a graph showing the growth of cassava seedlings under different treatments in test example 1 of the present invention.
FIG. 2 shows the survival rate of cassava seedlings under different treatments in test example 1 of the present invention.
FIG. 3 shows the proline content in the leaf samples of different treated seedlings of cassava in test example 2.
FIG. 4 is a DAB staining pattern of the leaves of seedlings of cassava under different treatments in test example 3 of the present invention.
FIG. 5 is a graph showing the results of experiments 3 of the present invention in which the leaves of seedlings of cassava were treated differently 2 O 2 And (4) content.
FIG. 6 shows the result of APX enzyme activity assay of leaves of seedlings of potatoes treated differently in test example 3 of the present invention.
FIG. 7 shows the POD enzyme activity measurement results of leaves of seedlings of potatoes treated differently in test example 3 of the present invention.
FIG. 8 shows the CAT enzyme activity assay results of leaves of seedlings of cassava under different treatments in test example 3.
FIG. 9 shows the results of MeAPX1 gene expression in leaves of seedlings of cassava under different treatments in test example 3.
FIG. 10 shows the results of MeCAT1 gene expression in leaves of seedling of cassava under different treatments in test example 3.
FIG. 11 shows the results of transcription levels of MeDREB2A gene of different treated leaves of seedling of cassava in test example 4.
FIG. 12 shows the results of transcription levels of the MeNCED3 gene from different treated leaves of a seedling of a potato in test example 4 of the present invention.
FIG. 13 shows the results of transcription levels of MeCBF3 gene of leaves of seedlings of cassava obtained by different treatments in Experimental example 4 of the present invention.
Detailed Description
In order that the invention may be more fully understood, reference will now be made to the following description. The present invention may be embodied in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
In one aspect of the present invention, there is provided a composition for improving drought resistance of cassava, including: beta-aminobutyric acid and gamma-aminobutyric acid, wherein the concentration of the beta-aminobutyric acid and the concentration of the gamma-aminobutyric acid in the composition are both 0.05 mM-0.3 mM. Preferably, the concentration of the beta-aminobutyric acid and the concentration of the gamma-aminobutyric acid are both 0.05mM to 0.15mM. Further, the concentration of the beta-aminobutyric acid and the concentration of the gamma-aminobutyric acid are both 0.08mM to 0.12mM. When the concentrations of the beta-aminobutyric acid and the gamma-aminobutyric acid are both 0.1mM, the effect of the preparation on improving the drought resistance of cassava is optimal.
In the present invention, beta-aminobutyric acid (. Beta.) -is-aminobutyric acid, BABA) belongs to the group of derivatized amino acids, a non-proteinogenic amino acid consisting of an amino group and a carboxyl group and four carbon atoms, which is structurally simple, the carboxyl group being on the first carbon atom and the amino group being on the third carbon atom. Beta-aminobutyric acid of the formula C 4 H 9 NO 2 Molecular weight of 103.12, also known as 3-aminobutyric acid. Gamma-aminobutyric acid (GABA) is one of the isomers of BABA, and the amino group of GABA is on the fourth carbon atom, is not bound to proteins, and is widely present in prokaryotes and eukaryotes. Mainly exists in the form of zwitterions, and has positive charges and negative charges. Both beta-aminobutyric acid and gamma-aminobutyric acid are readily available, inexpensive chemical reagents.
In another aspect of the present invention, there is provided a method for improving drought resistance of cassava, the method comprising: the preparation is used for irrigating cassava seedlings growing in soil for 3-8 days, the irrigation frequency is 4-6 days, and the irrigation amount is 15-25 mL/seedling each time.
When researching the cassava drought resistance preparation, the inventor of the invention finds that the preparation prepared by compounding beta-aminobutyric acid and gamma-aminobutyric acid at proper concentration can obviously reduce H when being irrigated to cassava seedlings stressed by drought 2 O 2 The results show that the preparation prepared by compounding beta-aminobutyric acid and gamma-aminobutyric acid acts on the cassava seedlings, and after a stress signal of the cassava to drought stress is triggered, survival advantage can be realized by increasing the content of organic osmotic proline, so that the survival rate of the cassava seedlings is greatly improved, and the accumulated beneficial effect on the drought resistance of the cassava seedlings is generated.
Experimental procedures without specific conditions noted in the following examples, generally followed by conventional conditions, such as Sambrook et al, molecular cloning: the conditions described in the Laboratory Manual (New York: cold Spring Harbor Laboratory Press, 1989), or according to the manufacturer's recommendations. The various chemicals used in the examples are commercially available.
The present invention is described in detail below with reference to specific embodiments and the attached drawings.
Example 1 formulation for improving drought resistance of cassava
In this embodiment, the agent for improving drought resistance of cassava comprises: 0.1mM beta-aminobutyric acid and 0.1mM gamma-aminobutyric acid.
The preparation method of the formulation of this example includes the following steps:
0.1031g of beta-aminobutyric acid and 0.1031g of gamma-aminobutyric acid were dissolved in 10L of water so that the final concentration of beta-aminobutyric acid was 0.1mM and the final concentration of gamma-aminobutyric acid was 0.1mM.
The application method of the preparation of the embodiment is as follows: the preparation of this example was applied to cassava seedlings growing in soil for 4 days, at an application time of 7 days and at an application rate of 20 mL/cassava plant.
Example 2 formulation for improving drought resistance of cassava
In this embodiment, the agent for improving drought resistance of cassava comprises: 0.25mM beta-aminobutyric acid and 0.05mM gamma-aminobutyric acid.
The preparation method of the formulation of this example includes the following steps:
0.258g of beta-aminobutyric acid and 0.052g of gamma-aminobutyric acid were dissolved in 10L of water so that the final concentration of beta-aminobutyric acid was 0.25mM and the final concentration of gamma-aminobutyric acid was 0.05mM.
The formulation of this example was used in the same manner as in example 1.
Example 3 formulation for improving drought resistance of cassava
In this embodiment, the agent for improving drought resistance of cassava comprises: 0.05mM beta-aminobutyric acid and 0.3mM gamma-aminobutyric acid.
The preparation method of the formulation of this example includes the following steps:
0.052g of beta-aminobutyric acid and 0.3093g of gamma-aminobutyric acid were dissolved in 10L of water to give a final concentration of beta-aminobutyric acid of 0.05mM and a final concentration of gamma-aminobutyric acid of 0.3mM.
The formulation of this example was used in the same manner as in example 1.
Example 4 formulation for improving drought resistance of cassava
In this embodiment, the agent for improving drought resistance of cassava comprises: 0.15mM beta-aminobutyric acid and 0.10mM gamma-aminobutyric acid.
The preparation method of the formulation of this example includes the following steps:
0.1547g of beta-aminobutyric acid and 0.1031g of gamma-aminobutyric acid were dissolved in 10L of water to give a final concentration of 0.15mM of beta-aminobutyric acid and 0.10mM of gamma-aminobutyric acid.
The formulation of this example was used in the same manner as in example 1.
Test example 1 influence of BABA and/or GABA on drought resistance of cassava seedlings
The test example studies the possible protective function of exogenous BABA and/or GABA on cassava seedlings under drought stress.
1. Test method
Culturing cassava variety 'south China No. 8' (SC 8) seedling on CBM culture medium, placing in incubator (16 h/8 h), temperature is 26 + -1 deg.C, and relative humidity is 70%. After three weeks of growth, seedlings with consistent growth vigor and good quality are selected and transplanted into gray plastic pots with bottom holes (each pot contains soil and four seedlings are planted), and normal ventilation is maintained by using an air pump. Growth was carried out under long-day (LD) conditions in a growth chamber at 27 ℃.
After 4 days of growth in the soil, 70mLH was added to each pot separately 2 O (Mock), 0.1mM BABA, 0.1mM GABA, 0.1mM BABA +0.1mM GABA solution, after 7 days of treatment, the remaining liquid was decanted and exposed to drought conditions to subject the seedlings to drought stress.
The test was set up for 3 replicates and statistical analysis was performed using Microsoft Excel and t-test to measure significance. P <0.05 was considered a statistically significant difference.
2. Test results
After 22 days, the survival rate of cassava seedlings under different conditions was counted, and the results are shown in fig. 1 and fig. 2.
The results showed that the persistent drought stress had an important inhibitory effect on the growth and survival of cassava seedlings, and after 15 days, almost all cassava seedlings in the control group withered (Mock group in fig. 1). However, the survival rate of cassava seedlings could be greatly improved with 0.1mM BABA, 0.1mM GABA, and 0.1mM BABA +0.1mM GABA, and the combined use of BABA and GABA had an additional beneficial effect on the survival and growth performance of cassava seedlings (FIG. 1).
At day 15, the survival rate of cassava seedlings under drought stress of the 0.1mM BABA group was 83%, the survival rate of cassava seedlings under drought stress of the 0.1mM GABA group was 83%, the survival rate of cassava seedlings treated with 0.1mM BABA +0.1mM GABA reached 100%, and was increased by 4 times compared to the control and by 20% compared to the single use (FIG. 2), compared to the survival rate of cassava seedlings under drought stress of the control (Mock).
Test example 2 proline content of cassava seedlings treated with BABA and/or GABA
This test example determined the proline content of cassava seedlings under treatment with BABA and/or GABA. Grouping and handling methods were the same as in test example 1.
For determination of proline content reference is made to the method of Bates et al (Bates Waldren et al, 1973).
Grinding the different treated cassava seedling leaves under liquid nitrogen into powder, adding 10mL of 3% sulfosalicylic acid solution, centrifuging for 12min at 10000g, then sucking 2mL of supernatant into a new 15mL test tube, adding 2mL of ninhydrin and 2mL of glacial acetic acid, and incubating for 45 min at 99 ℃. Taking out, cooling to room temperature, adding 5mL of toluene for extraction, mixing uniformly, and standing for 20-25 s. The chromophore containing toluene was aspirated from the aqueous phase and the absorbance read at 520nm and the proline content was obtained by standard curve. The results are shown in FIG. 3.
As can be seen from FIG. 3, under drought stress, proline content of cassava seedlings treated with Mock, 0.1mM BABA, 0.1mM GABA, and 0.1mM BABA +0.1mM GABA increased. However, the contents of proline in cassava seedlings treated with 0.1mM BABA, 0.1mM GABA, and 0.1mM BABA +0.1mM GABA were significantly higher than those in the control group (Mock) and increased with the increase of drought stress time. And when the BABA + GABA is used together for 27 days, the proline content is increased by about 2 times compared with a control group, and compared with a group treated by 0.1mM BABA and 0.1mM GABA, the proline content is increased by 8 percent and 24 percent respectively.
Test example 3 physiological indices of cassava seedlings treated with BABA and/or GABA
The test example determines various physiological indexes of cassava seedlings under the treatment of BABA and/or GABA. Grouping and processing methods were the same as in test example 1.
1. DAB dyeing
Plants exposed to drought conditions are responsible for the formation of Reactive Oxygen Species (ROS), such as superoxide (O) 2 - ) Hydrogen peroxide (H) 2 O 2 ) And hydroxyl (. OH) to be susceptible to oxidative stress. Cassava seedlings were therefore tested for ROS, cassava leaves were stained with 3,3' -Diaminobenzidine (DAB) for 24h and then destained with ethanol (Romero-Puertas, rodri guez-Serranoet al, 2004). The results are shown in FIG. 4.
From fig. 5 it can be observed that the brown precipitate of cassava seedlings under BABA, GABA, BABA + GABA treatment is less pronounced, especially the BABA + GABA combination is better, compared to the control (Mock) group. The exogenous BABA and GABA can reduce the oxidative damage of the cassava seedlings under drought stress.
2、H 2 O 2 Determination of the content
H 2 O 2 The determination of the content is referred to the method of Velikova et al (Velikova et al.).
Grinding the differently treated leaves of the cassava seedlings into powder under liquid nitrogen, adding 5mL 0.1% TCA homogenate and centrifuging 10000g for 12min. 1mL of the supernatant was added to 1ml of 10mM potassium phosphate and 2ml of 1M potassium iodide (KI), the absorbance of the reaction was read at 390nm, and H was obtained by a standard curve 2 O 2 And (4) content. The results are shown in FIG. 5.
As can be seen from FIG. 5, H in leaves under drought stress 2 O 2 High accumulation, by exogenous BABA and GABA, H 2 O 2 Is kept at a relatively low level, and the combination of BABA and GABA is more effective than the use of BABA or GABA, H alone 2 O 2 The concentration of (c) was reduced by 4% and 7%, respectively.
3. Activity of antioxidant enzymes
a. APX enzyme Activity assay
APX enzyme Activity measurement was measured by the method of Noctor et al (Noctor, mhamdi et al 2016).
The 3mL reaction system included: 50mM PPB (pH 7.0), 1mM ascorbic acid (AsA), 2.5mM H 2 O 2 And 100. Mu.L of each treated cassava seedling, the rate of decrease in OD value was measured at 290nm, once every 30 seconds for 3min. The enzyme activity was calculated by the change in absorbance per minute. The results are shown in FIG. 6.
b. Peroxidase (POD) Activity assay
POD activity was determined by the guaiacol method (Shao-xi, yi-rou et al, 1997).
The 3mL reaction system included: 0.75% of 2 O 2 0.25% guaiacol, 50mM PBS (pH 6.5) and 100. Mu.L of the enzyme extract of each treated cassava seedling. The rate of decrease of OD was measured at 460nm every 30s for 3min. Enzyme activity passes H within 180 seconds 2 O 2 Is determined by the reduction in. The results are shown in FIG. 7.
c. Peroxidase (CAT) Activity assay
CAT enzyme activity was measured by the hydrogen peroxide method (Dhindsa, plumb-Dhindsa et al 1981).
The 3mL reaction system included: 50mM PBS (pH 7.0), 45mM H 2 O 2 And 100. Mu.L of the enzyme extract of each treated cassava seedling. The rate of decrease of OD was measured at 290nm, every 30s for 3min. Enzyme activity through H within 180 seconds 2 O 2 Is determined by the reduction in. The results are shown in FIG. 8.
As can be seen from FIGS. 6 to 8, all antioxidant enzyme activities (including APX, POD and CAT) showed similar induction during drought stress. POD activity did not increase significantly in control (Mock) seedlings after 16 days of water deficit, whereas addition of exogenous BABA or GABA resulted in POD activity from 30U mg -1 The protein is increased to 75U mg -1 Protein, when exogenous BABA is combined with GABA,POD enzyme activity to approximately 100U mg -1 . The above results indicate that BABA and GABA can promote and enhance the antioxidant enzyme system of cassava seedlings under drought conditions to combat this abiotic stress.
4. Expression of antioxidase-related genes MeAPX1 and MeCAT1
After drought stress for 6 hours, sampling, extracting RNA, carrying out RT-qPCR after reverse transcription to verify the expression of antioxidant enzyme related genes MeAPX1 and MeCAT1, and the result is shown in figure 9 and figure 10.
The results showed that under drought stress, meAPX1 and MeCAT1 gene expression was significantly up-regulated, and BABA and/or GABA treated cassava seedlings had MeAPX1 and MeCAT1 transcript levels 4.6 and 4.1 times higher than the control group (Mock). Under the induction of the treatment combining exogenous BABA and GABA, the MeAPX1 and MeCAT1 genes are expressed higher. These results indicate that exogenous BABA and GABA induce the up-regulation of antioxidase-related genes, indicating that they can significantly alleviate reactive oxygen species-related damage of seedlings under drought stress conditions.
Test example 4 expression of drought-related genes in cassava seedlings treated with BABA and/or GABA
To further elucidate the possible molecular mechanisms of BABA and GABA participating in drought stress response, samples were taken 6h after drought treatment, RNA was extracted, reverse transcription was performed, and RT-qPCR was performed to examine the expression of several drought response genes MeDREB2A, meNCED3 and MeCBF 3. The results are shown in FIGS. 11 to 13.
The results show a significant up-regulation in the expression levels of medreeb 2A, meNCED3 and MeCBF3 under drought stress. Under drought stress, exogenous use of BABA and/or GABA significantly upregulated the expression of these three genes compared to the control group (Mock). The combined use of BABA and GABA has an additive effect.
These results indicate that exogenous BABA and GABA treatment can improve drought resistance by up-regulating the transcriptional levels of stress response genes including medeb 2A, meNCED3 and MeCBF 3.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is specific and detailed, but not to be understood as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (8)
1. The application of the composition in improving drought resistance of cassava is characterized in that the composition consists of 0.05mM to 0.3mM beta-aminobutyric acid and 0.05mM to 0.3mM gamma-aminobutyric acid.
2. The use according to claim 1, wherein said composition consists of 0.05mM to 0.15mM β -aminobutyric acid and 0.05mM to 0.15mM γ -aminobutyric acid.
3. The use according to claim 2, wherein the composition consists of 0.08mM to 0.12mM β -aminobutyric acid and 0.08mM to 0.12mM γ -aminobutyric acid.
4. The method for improving the drought resistance of cassava is characterized by comprising the following steps: the preparation for improving the drought resistance of cassava by irrigating cassava seedlings comprises active ingredients of 0.05mM to 0.3mM beta-aminobutyric acid and 0.05mM to 0.3mM gamma-aminobutyric acid.
5. The method for improving drought resistance of cassava according to claim 4, wherein the active ingredients of the preparation are 0.05mM to 0.15mM beta-aminobutyric acid and 0.05mM to 0.15mM gamma-aminobutyric acid.
6. The method for improving the drought resistance of cassava according to claim 5, wherein the active ingredients of the preparation are 0.08mM to 0.12mM beta-aminobutyric acid and 0.08mM to 0.12mM gamma-aminobutyric acid.
7. The method for improving the drought resistance of cassava according to any one of claims 4 to 6, wherein the cassava seedlings are seedlings which grow in soil for 3 to 8 days.
8. The method for improving the drought resistance of cassava according to claim 7, wherein the irrigation frequency is 4-6 days, and the irrigation amount per time is 15-25 mL/plant.
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| 于立尧 ; 姚琪 ; 李彭丽 ; 翁金洋 ; 高杏 ; 牛庆良 ; .外源γ-氨基丁酸对干旱胁迫下甜瓜幼苗生长的影响.上海交通大学学报(农业科学版).2018,(第05期),全文. * |
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