CN108619213A - 鬼箭羽抗ev71病毒的有效部位和制备方法 - Google Patents
鬼箭羽抗ev71病毒的有效部位和制备方法 Download PDFInfo
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Abstract
本发明属于中药活性成分生产提取工艺，涉及鬼箭羽抗肠道EV‑71病毒有效成分有效部位的鉴定和制备方法以及在制药领域的应用。具体涉及利用抗病毒追踪的方式提取制备鬼箭羽抗肠道EV‑71病毒的有效部位。具体步骤包括水回流提取鬼箭羽，抗病毒和体外抗病毒实验，确定其有效部位分别为：水提部位可以抑制肠道EV‑71病毒。本发明采用了抗病毒追踪的方法发现了鬼箭羽中抗肠道EV‑71病毒有效部位，其作为天然提取物具有安全性高等特点。
Description
技术领域
本发明涉及中药活性成分生产提取工艺，涉及鬼箭羽抗病毒有效部位提取的新思路、新方法以及在制药领域的新用途，具体涉及利用抗病毒追踪的方式提取鬼箭羽，确认鬼箭羽中抗肠道EV-71病毒有效部位。
背景技术
多年来世界各地都饱受各种病毒的入侵，比如SARS病毒，甲型H1N1等，每一次病毒的爆发都会引起人类的恐慌以及死亡。所以找到合适的抗病毒药物迫在眉睫。
肠道病毒71型简称EV-71， 属于微小病毒科中的肠病毒群， 对于中枢神经系统有极高的感染性，故临床上出现的症状包括脑炎、无菌性脑膜炎、急性无力肢体麻痹、泡疹性咽峡炎、手足口症、肌肉僵直、急性出血性结膜炎、发烧、肌肉僵直、呕吐等，其中以手足口症以及泡疹性咽峡炎最为常见。
鬼箭羽为卫矛科植物卫矛具翅状物的枝条或翅状附属物，又名卫矛、神箭、鬼箭、麻药、八树、四面戟、箆箕柴等。性寒，味微苦，入厥阴肝经。鬼箭羽对于多种疾病均有较好疗效，现已知的药理作用有降血糖、调血脂、抗氧化、抗菌、抗过敏等。
发明内容
本发明所要解决的技术问题是明确了鬼箭羽抗肠道EV-71病毒有效部位，并为该有效部位的确认提供了一种利用抗病毒追踪的方式制备鬼箭羽抗病毒有效部位的方法。水提有效部位可用于抗肠道EV-71病毒的治疗。
本发明的优点，本发明首次确认鬼箭羽具有抗肠道EV-71病毒的有效部位。并发明了一种抗病毒追踪的方法对该有效成分进行了制备，制备得到的有效部位对肠道EV-71病毒具有较好的治疗作用。
本发明发现鬼箭羽抗肠道EV-71病毒的有效部位为水提部位可以抑制肠道EV-71病毒；有效部位的制备方法，包括如下步骤：
（1）取一定量的鬼箭羽药材用10倍的水加热回流提取次，第一次2小时，第二次1.5小时；
（2）合并两次的水提取液减压浓缩，浓缩至每1毫升药液含有1克的药材；
（3）将（2）得到的浓缩液进行体外抗病毒实验，实验结果显示有很好的抗病毒效果。
具体实施方式
（1）称取一定量的鬼箭羽加10倍量蒸馏水加热回流提取两次，第一次2小时，第二次1.5小时，合并两次提取液，减压浓缩至每1毫升药液含有1克的药材。
将（1）中得到的浓缩液进行体外抗病毒实验，实验结果显示水提部位可以抑制肠道EV-71病毒并有很好的抗病毒效果。
Claims (4)
1.一种鬼箭羽中具有抗肠道EV-71病毒的有效部位和一种利用抗病毒追踪的方式提取分离的制备工艺方法，其特征在有效部位分别为：水提部位可以抑制肠道EV-71病毒。
2.根据权利1要求所述提取分离方法，其特征在于鬼箭羽水回流提取的料液比为1:10，提取次数两次，提取时间为第一次2小时，第二次1.5小时。
3.根据权利1要求所述提取分离方法，其特征在于水提液的浓缩液浓度为1ml药液含有1g的药材。
4.根据权利要求所述提取分离方法，其特征在于将鬼箭羽进行抗病毒实验，得到鬼箭羽抗病毒的有效部位分别为：水提部位可以抑制肠道EV-71病毒。
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