CN108605945A - A kind of bactericidal composition containing bromothalonil and amisulbrom - Google Patents

Abstract

The present invention relates to a kind of bactericidal composition containing bromothalonil and amisulbrom, active ingredient are bromothalonil and amisulbrom binary built.Wherein the mass ratio of bromothalonil and amisulbrom is 1~25: 25~1, the quality parts ratio 1%~80% of active ingredient bromothalonil and amisulbrom in preparation,, to allow the auxiliary element for using and receiving in pesticide, the dosage form of bactericidal composition of the present invention is missible oil, suspending agent, wettable powder, aqua, water dispersible granules, aqueous emulsion, microemulsion, granule, microcapsule formulations for remaining.It is mainly used for preventing the diseases such as cucumber, downy mildew of garpe, gray mold.

Description

A kind of bactericidal composition containing bromothalonil and amisulbrom

Technical field

The present invention relates to technical field of pesticide combination, more particularly to a kind of sterilization containing bromothalonil and amisulbrom Composition.

Background technology

Bromothalonil be a kind of wide spectrum, efficiently, the fungicide of low toxicity, can inhibit and root out the growth of fungi, bacterium, algae, There is preferable control effect to corps diseases, has special efficacy to anthracnose.It is widely used in fruit tree, grape, vegetables, cotton, flower The various crops such as life, watermelon, tobacco, tea tree, flowers, prevention anthracnose, scab, shot hole, powdery mildew, rust, damping-off, A variety of fungoids such as samping off, rhizome maize ear rot, canker, bacterial wilt, angular leaf spot, bacteroidal disease.Application mode is flexible, leaf Face spraying, seed treatment and soil pouring root, all show preferable preventive effect.

Amisulbrom belongs to contact killing type fungicide, has very high activity to epidemic disease and downy mildew, especially to germ Free spore activity is very high, can inhibit fungi breathing and spore germination, is a medicament aimed at prevention.Mainly act on horse Bell potato epidemic disease, downy mildew of garpe, cucurbit downy mildew, vegetables clubroot etc..

It is the most effective means of controlling plant diseases using chemical agent.But it applies to long-term continuous high dose single Chemical bactericide, be easy to cause the problems such as residual of medicament, environmental pollution and drug-resistant fungi develop.Rational chemistry Bactericide compounded or mixture have expand fungicidal spectrum, improve control effect, extend the optimum period for applying fertilizer, reduce dosage, reduce poisoning, The positive features such as residual, the generation for delaying drug resistance of fungi and development are reduced, bactericide compounded is to solve above-mentioned ask One of the maximally efficient method of topic.Develop new product fungicide price is constantly soaring, and in contrast, exploitation with research efficiently, Low toxicity, the compounding of low-residual and mixture have that small investment, the lead time is short and is paid attention to both at home and abroad, and increasing one after another develops Dynamics.We filter out bromothalonil and are compounded with amisulbrom based on the indoor screening and field trials, have bright Aobvious synergistic effect.And the compounding about bromothalonil and amisulbrom bactericidal composition and application there is no people to report at present It crosses.

Invention content

Based on the above circumstances, present invention aims at provide a kind of new and effective pesticide sterilizing composite.It is mainly used for Prevent the diseases such as cucumber, downy mildew of garpe, gray mold.

The technical solution of the present invention is achieved by the following measures：

A kind of bactericidal composition containing bromothalonil and amisulbrom, the active ingredient bromothalonil of the bactericidal composition with Amisulbrom two-spot compounds, remaining is auxiliary element.Active ingredient bromothalonil and indazole sulphur in the wherein described bactericidal composition The mass ratio of bacterium amine is 1~25: 25~1, and the bactericidal composition of the present invention is through toxicity test experimental verification, bromothalonil and Yin When the mass ratio of azoles flusulfamide is 1~10: 10~1, synergistic effect is preferable.

The formulations of pesticide that the bactericidal composition of the present invention can be prepared are missible oil, suspending agent, wettable powder, water Agent, water dispersible granules, aqueous emulsion, microemulsion, granule, microcapsule formulations.Wherein active ingredient bromothalonil exists with amisulbrom Gross mass in preparation accounts for the 1%~80% of the entire quality of the pharmaceutical preparations, wherein when accounting for 10%~60%, toxicity and residues reach preferable Balance, cost is relatively low.

The specific embodiment for the formulations of pesticide that bactericidal composition of the present invention is configured to is as follows：

The bactericidal composition is cream preparation, and the mass fraction of component is：1~25 part of bromothalonil；Amisulbrom 1 ~25 parts；10~30 parts of conventional emulsifier；20~50 parts of Conventional solvents；1~5 part of conventional synergist.The cream preparation it is specific Production stage is to add emulsifier, synergy after being first completely dissolved in active ingredient bromothalonil and amisulbrom addition solvent Agent stir evenly after at homogeneous and transparent oily liquids, it is filling, you can the missible oil preparation made into the composition of the present invention.

The bactericidal composition is suspending agent, and the mass fraction of component is：1~25 part of bromothalonil；Amisulbrom 1~ 25 parts；5~20 parts of dispersant；1~5 part of antifreezing agent；0.1~2 part of thickener；0.1~0.8 part of antifoaming agent；Penetrating agent 0~10 Part；0.1~5 part of pH adjusting agent；Water, surplus.The specific production stage of the suspending agent is first to mix other auxiliary agents, through high speed Shear-mixed is uniform, and active ingredient bromothalonil and amisulbrom, abrading-ball 2~3 hours in ball crusher, which is added, keeps a diameter equal In 5mm hereinafter, can be made into the aqueous suspension formulations of the present composition.

The bactericidal composition is wettable powder, and the mass fraction of component is：1~25 part of bromothalonil；Indazole sulphur bacterium 1~25 part of amine；3~10 parts of dispersant；1~5 part of wetting agent；Filler, surplus.The specific production stage of the wettable powder is： Active ingredient bromothalonil is mixed with amisulbrom and dispersant, wetting agent and filler by above-mentioned formula, in a stirring kettle Uniform stirring is being uniformly mixed, you can the wettable powder of the present composition is made after airslide disintegrating mill.

The bactericidal composition is water dispersible granules, and the mass fraction of component is：1~25 part of bromothalonil；Indazole sulphur bacterium 1~25 part of amine；3~10 parts of dispersant；1~10 part of wetting agent；1~5 part of disintegrant；Filler, surplus.The tool of the water dispersible granules Body production stage is：By above-mentioned formula by active ingredient bromothalonil and amisulbrom and dispersant, wetting agent, disintegrant and Filler is uniformly mixed, and is crushed with micro jet, through mediating, be then granulated in addition fluidized-bed granulation dryer, Through sampling analysis after dry, screening, you can the water dispersible granules of the present composition are made.

The bactericidal composition is aqueous emulsion, and the mass fraction of component is：1~25 part of bromothalonil；Amisulbrom 1~ 25 parts；3~30 parts of emulsifier；5~15 parts of solvent；2~15 parts of stabilizer；1~5 part of antifreezing agent；0.1~8 part of antifoaming agent；Thickening 0.2~2 part of agent；Water, surplus.The specific production stage of the aqueous emulsion is：First by bromothalonil and amisulbrom, solvent and breast Agent, cosolvent are added together, and make to be dissolved into uniform oil phase；By part water, antifreeze, the others pesticide such as antimicrobial Auxiliary agent mixes into uniform water phase；The oil phase is added to the aqueous phase while being stirred in the reactor at high speed, and slowly plus water is straight It to phase inversion point is reached, opens cutter and carries out high speed shear, and remaining water is added, shearing about half an hour, form oil-in-water type Aqueous emulsion, you can the aqueous emulsion of the present composition is made.

The bactericidal composition is microemulsion, and the mass fraction of component is：1~25 part of bromothalonil；Amisulbrom 1~ 25 parts；10~30 parts of emulsifier, 1~8 part of antifreezing agent, 0.5~10 part of stabilizer, 20~50 parts of Conventional solvents cosolvent.By bromine Bacterium nitrile is completely dissolved with amisulbrom with cosolvent, adds the other compositions such as emulsifier, antifreezing agent stabilizer, uniformly mixed It closes, is eventually adding water, microemulsion can be made into after being sufficiently stirred.

The bactericidal composition is granule, and the mass fraction of component is：1~25 part of bromothalonil；Amisulbrom 1~ 25 parts；1~10 part of wetting dispersing agent；0.1~5 part of thickener；0.1~5 part of antifoaming agent；Castor oil, surplus are supplied.Will effectively at Divide each components such as bromothalonil and amisulbrom, dispersant, stabilizer, antifoaming agent and solvent to be mixed according to the formula, is put into In sand milling kettle after grinding, it is sent into mixing in mixer for well-distribution and gets product.

The bactericidal composition is aqua, and the mass fraction of component is：1~25 part of bromothalonil；Amisulbrom 1~25 Part；0.1~30 part of wetting dispersing agent；2~8 parts of antifreezing agent；0.1~1 part of antifoaming agent；0.1~3 part of thickener；Spreader-sticker 5~15 Part；Water, surplus are supplied.Active ingredient bromothalonil is completely dissolved with amisulbrom, with cosolvent, dispersant is added, prevents Freeze the other compositions such as agent, antifoaming agent, uniformly mix, water is eventually adding, after being sufficiently stirred, you can obtains finished product.

The bactericidal composition is microcapsule formulations, and the mass fraction of component is：1~25 part of bromothalonil；Amisulbrom 1 ~25 parts；5~20 parts of urea；5~20 parts of formaldehyde；5~20 parts of emulsifying dispersant；1~5 part of antifreezing agent；0.1~2 part of thickener； 0.1~0.8 part of antifoaming agent；Water, surplus.Urea and formaldehyde (the amount ratio of substance are added in three-necked flask with a mixing device About l：1.5~2.0) pH value for, adjusting solution with sodium hydroxide solution then heats to 70~80 DEG C, instead to 8~9 or so It should obtain stable urea resin prepolymer.A certain amount of bromothalonil is taken to be dissolved in hexamethylene with amisulbrom, and in solution Middle addition emulsifying dispersant is made into being vigorously stirred and stablizes breast by the O/W types of water phase of aqueous solution containing emulsified dispersant Liquid.Above-mentioned urea resin prepolymer is added in lotion, adjusts pH value, polymerization occurs under acid catalyzed conditions, makes oil Phase substance is wrapped, and forms microcapsule granule.Slowly heating, solidification, temperature control is at 40~50 DEG C, hardening time 1h. Suitable auxiliary agent is added in selection, you can obtains stable microcapsules.

Wherein above-described emulsifier is selected from calcium dodecyl benzene sulfonate and aliphatic acid polyethenoxy ether, alkyl phenol polyoxy Ethylene ether sulfosuccinates, styrylphenol polyoxyethylene ether, nonylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, Aliphatic acid polyethenoxy base ester, any one of polyoxyethylene aliphatic alcohol ether or more than one a mixture of arbitrary proportions.

The solvent is xylene or biodiesel, toluene, diesel oil, methanol, ethyl alcohol, n-butanol, isopropanol, rosin-based Vegetable oil code name is ND-45, solvent naphtha, dimethylformamide, dimethyl sulfoxide (DMSO), and the one or more in water equal solvent are appointed It anticipates than the mixture of composition.

It is LG-3, GY-D1252, GY-D1256, SNWGF-01, lignin that the dispersant, which is selected from polycarboxylate code name, Sulfonate code name is 201107,201108, alkylphenol polyoxyethylene ether methyl ether condensation sulfate, alkylsulfonate calcium salt, naphthalene Sulfonic formaldehyde condensation compound sodium salt, alkyl phenol polyoxyethylene ether, polyoxyethylene carboxylate, aliphatic amine polyoxyethylene ether, glyceride One or more of fat acid ester polyoxyethylene ether.

The wetting agent is selected from lauryl sodium sulfate, calcium dodecyl benzene sulfonate, and Nekal BX moistens bleeding agent F, Alkylbenzene sulfonate polyoxyethylene triphen phosphenylic acid salt dimly, spaonin powder, silkworm excrement are one or more in spaonin powder.

The disintegrant is selected from bentonite, urea, ammonium sulfate, aluminium chloride, citric acid, succinic acid, in sodium bicarbonate It is one or more.

The thickener be selected from xanthans, carboxymethyl cellulose, carboxyethyl cellulose, methylcellulose, aluminum magnesium silicate, It is one or more in polyvinyl alcohol.

The stabilizer is selected from sodium citrate, one kind in resorcinol.

The antifreezing agent is selected from ethylene glycol, and propylene glycol is one or more in glycerine.

The antifoaming agent is selected from silicone oil, silicone compound, C_10-20Saturated fat acids compound, C_8-10Fatty alcohol It is one or more.

The filler is selected from kaolin, diatomite, bentonite, attapulgite, white carbon, starch, in precipitated calcium carbonate It is one or more.

The present invention has apparent synergistic effect using bromothalonil and amisulbrom as the composite bactericide of active ingredient, prolongs The generation of slow key drug resistance, and production and application costs are reduced, it is mainly used for preventing cucumber, downy mildew of garpe, ash The diseases such as mildew.

Specific implementation mode

To make technical scheme of the present invention, purpose and advantage be more clearly understood, present invention following specific examples It illustrates, but the present invention is not limited to these examples.The effect experiment of the present invention uses indoor biometrics and field trial phase In conjunction with mode, unless otherwise instructed, ratio mentioned below all be quality parts ratio.

Embodiment：Bromothalonil and amisulbrom virulence experiment with different proportions.

1.1 reagent agent

85% amisulbrom active compound, 90% bromothalonil active compound, above-mentioned active compound is by extra large Lear medicine company limited liability company Research and development department provides.

1.2 experiment targets

Bromothalonil was never applied from Shandong Shouguang acquired with the cucumber potting of amisulbrom and same type and detached, supplied Try cucumber variety：Chang Chun Mi Ci.

1.3 test method

1.3.1 medicament is prepared

Acetone solution active compound is first used, suitable two active compound is made by several different ratios according to the result of preliminary experiment, It is diluted each of the treatment into several concentration gradients for use with acetone again.

By the cultivation of susceptible cucumber variety Chang Chun Mi Ci in pot for growing seedlings, it is placed in a greenhouse culture, plant grows to three to four leaves After phase, the blade of identical leaf age is acquired, the culture for gray mold of cucumber germ and measurement.

1.3.2 the culture of gray mold of cucumber germ and the preparation of spore suspension

Gray mold of cucumber germ is cultivated using plants method under conditions of 20 DEG C, 12h alternation of light and darkness, turns generation per 30d Culture 1 time.With the conidium on sterile water elution incidence of leaf when inoculation, it is l × 10 to be configured to spore concentration⁶A/mL's Suspension.

1.3.3 strains tested sensitivity testing

Toxicity test is carried out using leaf dish moisturizing method.The blade of acquisition is first prepared into the leaf dish of a diameter of 1.5cm, at random Mixing is respectively placed in the series concentration liquid configured and impregnates lh, 25 leaf dishes of each concentration, tests with the place of not adding medicine Reason is blank control, and after immersion, leaf is face-up placed on the blotting paper of identical liquor strength wetting, in leaf dish Liquid blot, by prepared spore suspension l0L be inoculated in leaf dish center, after being placed at room temperature for 5min, be placed in 20 DEG C, 12h It is cultivated under conditions of alternation of light and darkness, the onset area in leaf dish is measured after 10d, calculates EC₅₀。

The toxicity test of mixture is carried out according to mixed ratio with single agent virulence determination method.

The EC that will be managed everywhere in measurement according to Sun Yunpei's Method₅₀Value is converted into practical mixed toxicity index (ATI)；According to mixture Proportioning, obtain theoretical mixed toxicity index (TTI), the co-toxicity coefficient (CTC) of mixture be calculated according to the following formula.

Single dose toxicity index=(standard agent EC₅₀/ reagent agent EC₅₀)*100；

ATI=(standard agent EC₅₀/ reagent agent (mixed) EC₅₀)*100；

Toxicity index X B contents in mixed of the toxicity index X A of TII=A in content (%)+B in mixed (%)；

CTC=(ATI/TTI) * 100；

If co-toxicity coefficient is big by 120, show there is synergistic effect；If being significantly lower than 100 (80 or less), it is shown to be antagonism； Between 100~120, it is shown to be summation action.

2.1 toxicity test results

1 amisulbrom of table is with bromothalonil to the toxicity test of gray mold of cucumber

As can be seen from the table, the test result of different proportion proportioning shows to dilute equal table respectively in active ingredient ratio Reveal stronger synergistic effect, wherein with bromothalonil and amisulbrom for 1:10~10:Synergistic effect good suggestion pair when 1 Suitable proportion 1:10~10:1 or so mixture preparation carries out further field control effectiveness test, to evaluate its field practical application effect Fruit.

3. field trial prevents cucumber downy mildew

3.1 test method

Twice, equivalent clear water is sprayed in check plot for dispenser altogether for experiment.When making up a prescription, after first medicament is fully dissolved with a small amount of water, then Suitable quantity of water is added and carries out whole plant spray treatment, even spraying.It is all made of power driven sprayer when spray, operating pressure is 0.3~ 0.4MPa, nozzle aperture 1.3mm.Cell spouting liquid is 40~50kg/667m², continuous dispenser twice, 10 days intervals, by medicine Liquid uniformly sprays application to crop complete stool.

3.2 investigation method

It is experimental field located at Shandong Shouguang, it is experimental field to select natually morbid plastic tent cucumber, and soil fertility is medium, irrigation and drainage side Just.Using double line sampling method, take per cell at 5 points, look into 5 caves, and 2 blades in every plant of fixed middle part carry out fixed point inspection when investigation at every It looks into.

It is classified according to cucumber downy mildew grade scale, record total strain number, diseased plant number, severity, calculating diseased plant rate, Disease index and preventive effect.

Cucumber downy mildew grade scale is

0 grade：Blade disease-free spot；

1 grade：Lesion area accounts for 5% or less entire leaf area；

3 grades：Lesion area accounts for the 6%~10% of entire leaf area；

5 grades：Lesion area accounts for the 11%~25% of entire leaf area；

7 grades：Lesion area accounts for the 26%~50% of entire leaf area；

9 grades：Lesion area accounts for 50% or more of entire leaf area.

Experiment is investigated 3 times altogether, i.e. investigation of premedication disease index, preventive effect investigation in 10 days after the 1st medicine, after 2 medicines 10 days and Preventive effect investigation in 20 days.

3.3 poisoning are investigated

Whether continuous 7d range estimations medicament has poisoning to crop after dispenser.

3.4 drug effect computational methods

Sick leaf rate (the %)=disease number of sheets/investigation total number of sheets × 100

Disease index=∑ (the disease numbers of sheets at different levels × opposite value of series)/(investigation total number of sheets × 9) × 100

Control effect (%)=(1- (disease index after disease index × treatment region medicine before blank district medicine)/(blank pair According to disease index before disease index after area's medicine × treatment region medicine)) × 100.

3.4 field control effectiveness test result of the tests

2 each test medicine of table prevents the experiment effect of cucumber downy mildew

As shown in Table 2, bromothalonil combines the effect of prevention cucumber downy mildew with amisulbrom mixture than more significant, compounding The significant effect of preparation is better than two kinds of single doses, not only significantly improves the control effect for downy mildew, but also further extend Its lasting period, and generated without poisoning under continuous poisoning in 7 days observation.

Interpretation of result：

In conclusion the present invention contains the bactericidal composition of bromothalonil and amisulbrom, to cucumber downy mildew, gray mold There is good control effect, and it is safe to Target crops.Complex preparation not only increases preventive effect, and expands fungicidal spectrum, Use scope is widened, cost is reduced, plays the role of that a medicine is simultaneous to be controlled to multiple diseases, mitigates manpower and materials, improves productivity effect. So the invention of this complex preparation has a very important significance society with popularization.

Claims (4)

1. a kind of bactericidal composition containing bromothalonil and amisulbrom, it is characterised in that：The bactericidal composition it is effective at It is divided into bromothalonil and amisulbrom binary built, remaining is auxiliary element, wherein active ingredient bromothalonil and amisulbrom Mass ratio be 1~25: 25~1.

2. bactericidal composition according to claim 1, it is characterised in that：Bromothalonil and amisulbrom in the formulation total Weight accounts for the 1%~80% of the entire quality of the pharmaceutical preparations.

3. bactericidal composition according to claim 2, it is characterised in that：Bromothalonil and amisulbrom in the formulation total Weight accounts for the 5%~60% of the entire quality of the pharmaceutical preparations.

4. bactericidal composition according to claim 1 or 2 or 3, it is characterised in that：The dosage form of the bactericidal composition is breast Oil, suspending agent, aqua, wettable powder, water dispersible granules, aqueous emulsion, microemulsion, granule, microcapsule formulations.