CN108588259B - 与非洲稻落粒相关的插入缺失片段及其应用 - Google Patents

Abstract

本发明公开了一种与非洲稻落粒相关的插入缺失片段及其应用。本发明提供了用于鉴定或辅助鉴定水稻落粒性的成套引物，由引物对1(SEQ ID No.3和SEQ ID No.4)和引物对2(SEQ ID No.5和SEQ ID No.6)组成。本发明在非洲栽培稻中发现包含有SH3基因在内的45k插入缺失片段(SEQ ID No.1的第161‑45560位)与非洲稻落粒程度密切相关。该插入缺失片段未缺失类型的落粒性大于插入缺失片段缺失类型的落粒性。本发明对于筛选和培育落粒性适中的非洲稻新品种具有重要意义。

Description

与非洲稻落粒相关的插入缺失片段及其应用

技术领域

本发明属于植物基因工程领域，涉及一种与非洲稻落粒相关的插入缺失片段及其应用。

背景技术

稻属(Oryza L.)含有两个栽培种，即亚洲栽培稻(O.sativa L.)和非洲栽培稻(Oryza glaberrima Steud.)，它们分别独立起源于亚洲和非洲。亚洲栽培稻产量高，在世界各地广泛种植，而非洲栽培稻产量较低，仅限于西非地区。

非洲栽培稻是大约3000年前独立起源于非洲的栽培稻，和亚洲栽培稻是由不同的祖先物种驯化而来。16世纪中叶，葡萄牙人便将亚洲栽培稻引入西非地区，从而导致非洲栽培稻的物种急剧减少，所以现在非洲栽培稻的种植面积远小于亚洲栽培稻。目前，亚洲栽培稻已在全球范围内广泛种植，为全球近一半人口提供食物来源。非洲栽培稻主要分布于非洲西部，是当地主要的粮食作物之一，对非洲的文明和经济起到了积极的作用。然而，与亚洲栽培稻相比，非洲栽培稻的相关研究十分匮乏，对非洲栽培稻的群体遗传结构和进化历史知之甚少。

由于非洲栽培稻具有生长快、耐贫瘠、耐干旱、生产投入低等特点，所以在特殊生态环境中有一定的生产优势。因此，进一步加深对非洲栽培稻的研究对于提高非洲栽培稻的产量具有重要作用。

落粒性是与水稻生产密切相关的重要性状之一，太易落粒或太难落粒的水稻品种都不宜在生产上应用。与水稻落粒性相关分子标记及其基因的发掘将为了解水稻人工驯化积累新素材，也为育种应用提供新的基因资源。

发明内容

本发明的目的是提供一种与非洲稻落粒相关的插入缺失片段及其应用。

第一方面，本发明要求保护一种用于鉴定或辅助鉴定水稻落粒性的成套引物。

本发明所提供的成套引物对由引物对1和引物对2组成：

所述引物对1为如下(a1)-(a3)中任一所示的引物对：

(a1)由SEQ ID No.3所示的单链DNA和SEQ ID No.4所示的单链DNA组成的引物对；

(a2)由将SEQ ID No.3和SEQ ID No.4经过一个或几个核苷酸的取代和/或缺失和/或添加后所得序列所示的两条单链DNA分子组成的，且与(a1)中所述引物对功能相同的引物对；

(a3)由经SEQ ID No.1的第161-45560位所示核苷酸序列设计所得的引物对，且与(a1)中所述引物对功能相同的引物对。

所述引物对2为如下(b1)-(b3)中任一所示的引物对：

(b1)由SEQ ID No.5所示的单链DNA和SEQ ID No.6所示的单链DNA组成的引物对；

(b2)由将SEQ ID No.5和SEQ ID No.6经过一个或几个核苷酸的取代和/或缺失和/或添加后所得序列所示的两条单链DNA分子组成的，且与(b1)中所述引物对功能相同的引物对；

(b3)由经SEQ ID No.2所示核苷酸序列设计所得的引物对，且与(b1)中所述引物对功能相同的引物对。

第二方面，本发明要求保护一种用于鉴定或辅助鉴定水稻落粒性的引物对。

本发明所提供的用于鉴定或辅助鉴定水稻落粒性的引物对，为前文所述成套引物中的所述引物对1或所述引物对2。

在第一方面和第二方面中，所述引物对1和所述引物对2中的两条引物既可以分别单独包装，也可以等摩尔混合包装。

第三方面，本发明要求保护一种用于鉴定或辅助鉴定水稻落粒性的试剂盒。

本发明所提供的用于鉴定或辅助鉴定水稻落粒性的试剂盒中除了含有前文所述的成套引物或引物对外，还含有dNTP和DNA聚合酶。

所述试剂盒中的各物质可分别单独包装。

第四方面，本发明要求保护一种与水稻落粒性相关的分子标记。

本发明所提供的与水稻落粒性相关的分子标记，具体为SEQ ID No.1的第161-45560位所示的DNA分子。

第五方面，本发明要求保护前文所述的成套引物或引物对或试剂盒或分子标记在鉴定或辅助鉴定水稻落粒性中的应用。

第六方面，本发明要求保护一种鉴定或辅助鉴定待测水稻落粒性的方法。

本发明所提供的鉴定或辅助鉴定待测水稻落粒性的方法，具体可包括如下步骤：检测待测水稻的基因组中是否含有DNA片段甲，然后按照如下确定所述待测水稻的落粒性：含有所述DNA片段甲的水稻的落粒性为落粒；不含有所述DNA片段甲的水稻的落粒性为不落粒。在水稻种子成熟时，穗部籽粒脱落数占穗粒数总数的80％或以上，其表型为落粒，其余为不落粒。

其中，所述DNA片段甲具体为SEQ ID No.1的第161-45560位所示DNA片段。

进一步地，所述“检测待测水稻的基因组中是否含有DNA片段甲”的方法可为如下任一：

(A)包括如下步骤：用前文所述的成套引物中的所述引物对1和所述引物对2分别对待测水稻的基因组DNA进行PCR扩增，得到各自引物对对应的PCR扩增产物；检测所述PCR扩增产物；

若所述引物对1的PCR扩增产物含有大小为503bp的片段，且所述引物对2的PCR扩增产物不含有大小为2193bp的片段，则所述待测水稻含有所述DNA片段甲；若所述引物对1的PCR扩增产物不含有大小为503bp的片段，且所述引物对2的PCR扩增产物含有大小为2193bp的片段，则所述待测水稻不含有所述DNA片段甲。

(B)包括如下步骤：用前文所述的成套引物中的所述引物对1对待测水稻的基因组DNA进行PCR扩增，得到PCR扩增产物；检测所述PCR扩增产物；

若所述引物对1的PCR扩增产物含有大小为503bp的片段，则所述待测水稻含有所述DNA片段甲；若所述引物对1的PCR扩增产物不含有大小为503bp的片段，则所述待测水稻不含有所述DNA片段甲。

(C)包括如下步骤：用前文所述的成套引物中的所述引物对2对待测水稻的基因组DNA进行PCR扩增，得到PCR扩增产物；检测所述PCR扩增产物；

若所述引物对2的PCR扩增产物不含有大小为2193bp的片段，则所述待测水稻含有所述DNA片段甲；若所述引物对2的PCR扩增产物含有大小为2193bp的片段，则所述待测水稻不含有所述DNA片段甲。

其中，所述大小为503bp的片段具体为SEQ ID No.1的第20975-21447位所示DNA片段。所述大小为2193bp的片段具体为SEQ ID No.2的第8-2200位所示DNA片段。

第七方面，本发明要求保护前文所述的成套引物或引物对或试剂盒或分子标记或方法在水稻育种中的应用。

在第一至七方面中，所述水稻均可为非洲稻。在本发明的具体实施方式中，所述水稻具体为表1中所示的57份非洲稻品种。

实验证明，本发明在非洲栽培稻中发现包含有SH3基因在内的45k插入缺失片段(SEQ ID No.1的第161-45560位)与非洲稻落粒程度密切相关。该插入缺失片段未缺失类型的落粒性大于插入缺失片段缺失类型的落粒性。本发明对于筛选和培育落粒性适中的非洲稻新品种具有重要意义。

附图说明

图1为非洲野生稻W1411与非洲栽培稻IRGC104165的落粒性比较。

图2为SH3基因定位示意图。

图3为用不同的引物对分别扩增非洲野生稻W1411与非洲栽培稻IRGC104165得到的PCR产物进行琼脂糖凝胶电泳的结果。

具体实施方式

下述实施例中所使用的实验方法如无特殊说明，均为常规方法。

下述实施例中所用的材料、试剂等，如无特殊说明，均可从商业途径得到。

pCAMBIA1300载体：记载于“Zhu Zuofeng,Tan Lubin,Fu Yongcai,Liu Fengxia,Cai Hongwei,Xie Daoxin,Wu Feng,Wu Jianzhong,Matsumoto Takashi,SunChuanqing.Genetic control of inflorescence architecture during ricedomestication.Nature Commun,2013,4:2200doi:10.1038/ncomms3200.”一文，公众可从申请人处获得，仅可用于重复本发明实验使用。

非洲稻品种IRGC104165、非洲野生稻W1411和表1中非洲稻品种：其中“PI”编号开头的非洲栽培稻可以从美国农业部获得，其他编号可以从国际水稻研究所(网址：http://irri.org/)获得。

实施例1、调控非洲稻落粒基因的定位及插入缺失片段的确定

非洲野生稻W1411与非洲稻品种IRGC104165(图1)杂交构建了一个包含168个单株的F2分离群体。在这个F2群体中，落粒性都与分子标记RM5626与MR81紧密连锁，最终将SH3基因定位在这两个标记之间。然后将分离群体扩大到2650个单株，并根据标记RM5626与MR81之间的序列设计引物，最终将SH3基因定位到分子标记SNP29与SNP31这两个标记之间(图2)。在非洲栽培稻IRGC104165中，这两个标记之间的物理距离为17kb，在网站http://www.softberry.com/上根据非洲栽培稻IRGC96717(CG14)序列预测，该区间没有候选基因。在非洲野生稻W1411中，这两个标记之间的物理距离为63kb，与IRGC104165相比，这个区间有一个45kb片段的插入，在网站http://www.softberry.com/上根据非洲野生稻W1411序列预测，在这63kb的区间内有6个开放阅读框ORF，通过RT-PCR分析表明，在这6个ORF中只有ORF3在控制种子落粒性的离层部位表达，因此ORF3为SH3的候选基因，控制非洲栽培稻的落粒性状。

非洲野生稻W1411的SH3基因的基因组序列如SEQ ID No.7所示。对非洲野生稻W1411和非洲栽培稻品种IRGC104165的SH3基因附近区段测序发现，非洲栽培稻品种IRGC104165相对于非洲野生稻W141缺失了一个包含SH3基因在内的大小为45kb的插入缺失片段(SEQ ID No.1的第161-45560位)。具体试验方案如下：

一、引物设计

以非洲野生稻品种W1411的基因组为模板，设计如下引物对P1F/P1R。

P1F：5’-caattgccatttgcttaatactctt-3’；

P1R：5’-tgcatgcatacgatcactca-3’。

其中P1F对应SEQ ID No.1中第20975-20999位，P1R对应SEQ ID No.1中第21458-21477位，扩增片段大小为503bp。

以非洲栽培稻品种IRGC104165的基因组为模板，设计如下引物对P2F/P2R。

P2F：5’-ggagaagacgagctcacattg-3’；

P2R：5’-aattgatctgattcctgtga-3’。

其中P2F对应SEQ ID No.2中第8-28位，P2R对应SEQ ID No.2中第2181-2200位，扩增片段大小为2193bp。

以非洲野生稻品种W1411和非洲栽培稻品种IRGC104165的基因组为模板，分别以引物对P1F/P1R和P2F/P2R进行扩增，分别获得长度约为503bp和2193bp的片段。

二、PCR鉴定

将步骤一扩增得到的PCR产物进行琼脂糖凝胶电泳。

鉴定结果如图3所示，泳道1和4为DNA Marker III(北京拓英坊科技有限公司产品)，目的条带大小分别为503bp和2193bp。泳道2和5为非洲野生稻W1411，泳道3和6为非洲栽培稻品种IRGC104165，泳道2和3为引物对P1F/P1R扩增产物，泳道5和6为引物对P2F/P2R扩增产物。实验结果表明，插入缺失片段(SEQ ID No.1的第161-45560位)未缺失的W1411可用引物对P1F/P1R扩增出条带，而用引物对P2F/P2R不可扩增出条带；插入缺失片段缺失的IRGC104165用引物对P1F/P1R不可扩增出条带，而用引物对P2F/P2R可扩增出条带。

实施例2、插入缺失片段在鉴定现有非洲稻品种落粒上的应用

一、实验材料

利用来源分布于非洲西部多个国家的57个非洲稻品种为实验材料(具体见表1)，鉴定插入缺失片段在非洲稻落粒选择上的应用。

二、实验方法

将各供试植株单株挂牌取样后，提取其基因组DNA并以其为模板，用引物对P1F/P1R和引物对P2F/P2R分别进行PCR扩增，得到PCR产物后进行琼脂糖凝胶电泳，根据结果按照如下方法确定待测单株的基因型：若用引物对P1F/P1R可扩增出503bp大小条带(SEQ IDNo.1的第20975-21477位)，用引物对P2F/P2R未扩增出可见条带，则该该待测单株为插入缺失片段未缺失基因型；若用引物对P1F/P1R未扩增出条带，用引物对P2F/P2R扩增出2193bp大小条带(SEQ ID No.2的第8-2200位)，则该该待测单株为插入缺失片段缺失基因型。

P1F：5’-caattgccatttgcttaatactctt-3’(SEQ ID No.3)；

P1R：5’-tgcatgcatacgatcactca-3’(SEQ ID No.4)；

P2F：5’-ggagaagacgagctcacattg-3’(SEQ ID No.5)；

P2R：5’-aattgatctgattcctgtga-3’(SEQ ID No.6)；

同时，待非洲稻成熟后，考查并统计各单株的落粒性。在种子成熟时，穗部籽粒脱落数占穗粒数总数的80％或以上，认为其表型为落粒，其余为不落粒。

三、实验结果

结果如表1所示，可见：在所有的57份非洲栽培稻中，该插入缺失片段基因型均为缺失，且它们对应的落粒表型为不落粒。该结果表明在这57份非洲栽培稻中插入缺失片段为缺失的基因型对应的落粒性表型均为不落粒。

表1 57个非洲栽培稻品种落粒性

<110> 中国农业大学

<120> 与非洲稻落粒相关的插入缺失片段及其应用

<130> GNCLN180983

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<170> PatentIn version 3.5

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<213> Oryza barthii L.

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atctgtgaaa atcacttttc gtgcgctggt gtggtgcctg tttgaaaata tgccgatttt 60

tatagacatt tcggaggtga cgggtcgtcc agccatttgc aaaaataggt tttgttcatg 120

tgcgaaaatc cttttttaag tagtgcttct tgcagttagc tagcactact gctacataag 180

gcctgatatc taatcctacc tttgccacaa atttaaagag tgtaattctg gtcacgtcgt 240

aaattcattt aatttaacaa gctgtactcc ttaaatgtct tagaacacat aattactgat 300

gattataagt atacgagatt atgatttgga cgacctatgt tgtagtacat attgcacgaa 360

tataattcag tttctattgg aggccggagg ggcgtatata tagctaggtg gtcccaatgc 420

cagatgtgcc caccgttcca aaaaaaccaa tctagtactt aggatgtgat gggacacatc 480

ctagtacaac gtcatgtcag attcgttata ctaggatgtg tcccattcta gtcctaggtt 540

agattttttt ttttttgaga cggaggaagt aaaccattgc ctcatggaac taaaattact 600

ctctgttcaa ctcaaggtgc aattcatggg tgaggatgta aaaggaaatc tgttacaata 660

gttgaggagg taaaagcaga aaatttgtca aatagttgaa ggctgtactc tagaactttt 720

cctttaaacg aaccataaag tgatgccttc aaacgttcgt tcaaaaaaga ggtgccttca 780

aaaatacaat atgtaaaatc ttttacaggt aaaattagtt atatatagac ttcttttact 840

acacaaaaga cgactcacac gtacgtacac accactacgc acaccttcgc acacactcgt 900

aagtaagctt cctaacatgc atttgaagag gcggagatca gtgatagaga tccctggact 960

aactaattaa attcatgtta aaaacatact accacatatg taatatttgt gggtgtgcca 1020

gaaaaatcta gcaaatggac tcatgcactc atgaatcgac aaaccgcact gtttgaagtt 1080

cccaatttta gaccaaattt agtgtgacaa gaataggggt cctttgggaa acaaggttca 1140

aaaatagaaa tagaaaaaac gcagggtgat tacacgtgaa aacataaaga aaataaaaga 1200

aaaagagaaa ttttatggga cacatagcta agctaggttg tttgtaacat atgtgatgct 1260

ataccagcca gtgaaacaaa agaggtcgga gtggatatgt tagaatcctt taaaattcct 1320

tcgtttccta tgttctcacg gaaatgctag ctatttctta taacaattta tgctaaagaa 1380

aataggtaaa gtggcaaatt ccaagggaaa tccttgttag gttggcccta attattttgt 1440

tatatatttc tagagaggtt cgttgtttta ggatggatca atcgtttagc accatatata 1500

taatttcaga gcgaaagcat tcaaagataa cgtacccaac tcctcgcaaa tcacaagcac 1560

gcacgaaaaa actgatgatg caattaatta agctagggga aaataaaacc cctggcgatg 1620

gcgatgtgtc catcattttc tcgtggacac acgaccaagt tgcggggcga gagaaggaca 1680

aggcgcgggg ccggcagcga cacgtacgtt acgcacgcac acgcgacatc gcacatgcac 1740

aaggcaggca tgcatgcatg cgcgtcgtcg acgcctgtgg cccgctgctg cgctgggccc 1800

cgtccaccag cgaccgcgca tgcatgcgcg ctgctgcgac gtgtacccgc gccccgggct 1860

agctagctag cggcatcatg taggtactca tcgtcactgg cagtggcagg gcatatacta 1920

tatatgactc ttggtagagt tattttactc ttggtagaat tatatataaa taattccttc 1980

gtcttaaaat aaaacaaata aaataaaata tgatatagat ggcggcgggg atggcgacgg 2040

ctgggcggat ccagccgcgc cgaatccctg gagaaggatc tgcccacccg atggccagat 2100

cgggcaaggg cgaggatgta gtgggagagc cgtcgcggcg gcggtggccg gcagtcgtcg 2160

ccgcgatgag aatctacggc ggcacggagc tcacagcgac gacggtgtca gcgggtggcc 2220

gacaggagga gcgcaacagt tggttggctg gccagcaacg acgggcgacc gtggcacgac 2280

tggtggttgc gtaggccggc gaggcgcgga caacgtcggc ggggttggcc ggcggcggca 2340

gtggttggtg cagcgggaga tgagaggcgt gtgccattgc agcgtggttg gcgcaagcag 2400

tggccggtgc agtgagaggc gacaaggcgt ggccgatcgt ggcatgcgga ggctggcggg 2460

gggggcaccg gtgcagtggt gcccacgcgt cgacggaggt ttagatggca gtggagcatc 2520

gctgcgtcaa tggtggatag gcaggcagtt gatggcgaat gaaaacccag cccggcttta 2580

gccgagccgg caatgatggc gctcgagcgt cactccccct cctgaggacg ttgtcgtgct 2640

gtctctcacc ccctctagtg gggctgccgg gtgaaagccc agtcaaaatt tttgagacct 2700

cgacggacgg tggcagcggt gctttcgtca cttctctctc taaagatgcc atctaggcag 2760

cacccttgct gaaaccactc aaacaactac tgcaagctca ctagagtcac atcctcttgt 2820

attggcgctc tagtttttac aagttggcca catcggaggg ctagggggag agcggttcca 2880

ttcttctttc tcaccctctc tccctaaatc cctaagatgt ggttagagtg ggattttgtg 2940

ttggttgtaa gggttgagtt ttaggcgatc ccggttgtag ctcttttgtt ggactagcgg 3000

cggctggtcc gatgctagcc ttctcctggg tttgtgtttg gcgctttcgc tgtgtgggtg 3060

tggtatagtt tgcctcccaa gtttgtaatc ttgcaacttt tttctgatct atcaatatga 3120

acttcgcact gtcctgtgca ggtcgttaaa agtaaaagcc gtacctagtt tttaattcaa 3180

gtgtttgtga ccattttaat aatctctaaa tctcgacttt tgaattagta aaagtaaaat 3240

tagaccacga cctaccatcc aactaggcac cagggttcac tttatcggtt aacccctttt 3300

gatcggaggt cacctaaatc ctgaaatttc agaaattttg gtccgaaatt tcctaaattt 3360

tgaataaaat ttagtttaat ttgaacaaat attgctaaaa tttatgaaaa aattgaaaaa 3420

tttcggctga agtaatatcg tatcggaggg ggtccgaaat ttcggaaaaa tttctaaccc 3480

tgctaggcac gtactccttc cgttcttaaa tggtatctga atatattttt atagaaagga 3540

agagagcgta tgggtaatga atgaatgatc tatgcagagt tgctgcgata aagaatgacg 3600

catgggtcta tgcatgctca cataatgatg ggagaagaga ctgaatactg agtgaaccag 3660

tagatcggca gatgcagagg gagaggtgga tcgaaagcgt cccgtcgaca cgacacgaga 3720

tggcaatggc aatggcatgg ctctgtctcc atcactacgg accccgcacc acctgcgcca 3780

aaaatgctcc ctgcccctgc ttgtctcagc tcctgtacac cgcgcactgg accgcgagaa 3840

cctcacaagc ctcagatcga tgctgatcta tccccaccgg tcagtgtgct caatttgcta 3900

tgtgtcctat gcttatttgg tactccctcc ctccgtttca ggttgtaata ccttttcact 3960

taatttggtc aaagtcaaac agctttaagt ttagtaatat tttcaatcta agataaattt 4020

attataaaaa tatattcaat taatgatttg atgaaactaa tttagtatta tcaatattac 4080

tatatttgtc tataaactta agttaaactt aaaacagttt gactttgacc aaagtcaaaa 4140

cgtcttataa cctaaaacga agcgtgcgta ccctcttttg tatgctcctt ctatagctag 4200

atcgatatgt atatggaaag ccatgaagat ggttattcaa aaactcagag atgtttcgga 4260

gtaatgttgc aaaataaaca gaccgtctgt attaatatat tcttgcattt gcttacttta 4320

actaggagtg tccttcgtct ttaactagga gtgtccttcg tctctaacat ccaggtcctt 4380

gataaaagag agagtacggc atactttctt atccctcata tataaccgtg tgacacatgc 4440

aacacattat tggttatttt agcttttctt taccataaaa atgtacacat cattgttccc 4500

tccattttat aaataagacc aaatattaat atattctcgc atttccttac tttaagagta 4560

tctttcatct ctaacaaata ggtccttaac gagagagaaa gagagagcga cacaccatct 4620

ttttccctca ctatctcttt cccacatgga caactgtatg gcacatgcaa catattattg 4680

gttattttag cttttctttg ccatgcaaac atacacgtca atgttccctc cattttatat 4740

gagcttaata ttattttcgt gtttgatata tcaggaacac acgtggtacc aaataattta 4800

atttattcat ctttcattga agttttttct cgattatcgc gtatctcttt ctgtggagta 4860

ctttcaagca tttatatcgg taaccagtgg cggagccgac agcactgggc cagtgcttgg 4920

gctctgcaac atgttgagat acaatactaa ttacttactc tgattaatta gttggtgcta 4980

atggaggtta gattagctag gaaaactagc aaaaagcaag gctccagttt tgatccctca 5040

agggagatca tttcgtctta tttttctcat gactatttgc attatacttg cggtaaaaaa 5100

tgtaaatggt gggatgttaa tccattcgat ctctaataat agctccatag tctagcccat 5160

cggtaatata caactaccat ctcatcaata taatcccctc gcaccggcta ctagaataga 5220

gcatcccagc ctttgcttaa atagctacag ccaattatta taatctcaca cttaaaatga 5280

gtgaagttgg atttgcgggt atatctgtga tcctctgtta ggctcgtcca aactcaagaa 5340

aatctctagc tccaccactg tcgggaacat caggcttctc tctagcttgt ttcagctact 5400

atctatagcc ataaaattgt ggcattttca tcatatttta agaacttaat ttcaatgtca 5460

atatctctta atatttaggt ggatgtgtga gaattatatg tgtacatatt ttttaaaaaa 5520

tattttcata atattataat ttatctaatt atataaatga ataaattata ctcatggtac 5580

ataaacttaa tagatgggtg taagttagca cacagacttg taaaatgttt gttttggtgc 5640

atgaattagt ctgatgcttg taaatcaagc ccaaatgaga atgctatgca tggttagaac 5700

taatttgaag gcttattttg atgctaggat gacacatagg tacagatatt aggacggtgc 5760

cctctacaca tgcatgtgct cagtcaaaca tgcaatagga atgcttatca tatagaaaga 5820

ttataaaata tagattaagg atgcaatgaa ttgaaaagag gggaaaaaaa tacaaagtag 5880

agattgtttt tgcacatgta gcatgactca tgcccccccc taaattatgt gtacgcgttt 5940

cattataaag taggttccaa ctcagcatca ttagtcacta catgttattt atgccttgtt 6000

tcgcatgcac tagacaagtt catacaacaa aatgaacggt ttacaagttt gggtacaagg 6060

ttgcacccac ctgtcaagtt catgtttcac caatgcaatc tactataaat aaatataatg 6120

caatagaaac caattatcaa ctaatattta aggatttatc tacgtctcaa atggcatgca 6180

agttcgccta gcactccgaa ctttaaaatt ggatatccaa tcactaataa aattatttgg 6240

ataatggttt tgcgtatttg aatgtttaaa tgagaagttt tgaataacta acatagcatg 6300

tttatgtatc atcaatcatc aattgccaat ttatactcta tataatgtca taatggtatt 6360

atattgacat tcctttgtaa gtgagagaaa aaacagagta aaaagaaaga tgtaggtgaa 6420

gattttaata tatatgtcaa attcatatgc tatgtagcca aactcattca acatatatgc 6480

aaattaggta aaaaaaaaac cactttgcta aaccatctta gggtgtaata tgagcggtat 6540

tgtttagttc agggggtctg atgtctgttc cagagtttag cgtgctaaat ggattatcaa 6600

tcaaagttaa ggggatattt agactttttt ctatcatcaa tcatcggtca ccaatttata 6660

ctctatagta atgtcataat agtattatat ctacaatatt cctttgtaag tgagaaaaaa 6720

tggagtagaa tagaaatatt taggtgaaga ttttaatata tatgtatgtt caattcatat 6780

gccaagtggt caaactcaac tatcatatat gcaactatca tatatgcaaa tttaggtaaa 6840

aatacacttt ataaaaccat cttaaggtat aatataaatg gtattgtata atttaggagg 6900

atggatattc ggtttcagag ttgagggtac tatatagact agttcatggg tatttagact 6960

tttttttaag aacgttattc ttttttttaa tctctctttc tttattggct tcataactca 7020

atactttttg aaaatacaag ggaacaccac atgacacata gtagtactac tgtttagaat 7080

tgatgggggc cagctaccaa actccatcac actaccaata ttacgtacac atatatatag 7140

ctgctacatg tatattggcc gtgcaaaatt ggctgtatag gttccgagac aaggcaacac 7200

aaccacgaat taatccaaaa atgatgaata ttactattaa ttatggtacg aaaagaaaat 7260

gtaagtgaga tatgcatcaa ccgtacgtac gtagtcctgc tactttggtt aatcattaac 7320

tggtaaagaa aatgtttgac tgcattaata tatatcaatt gaaaaggcca aacgtgtaca 7380

ggccgcgtga atgagcgata tgtgttaatt agtttcaatt tcctagtact gttaagagtg 7440

ggtgtcacat tcactaatcg atcgtatggc atggagtgcg aaatagcctt gcaaaaagtc 7500

tctcgaatcg gacttctcac tggccattga tcgaattatt gactgcttca agatctgggc 7560

ggggtgaggc agacacaaat tcgaccgaga aaactacctg agaaaacaag caagacaaga 7620

tcgcgatctg gacagggaga gaaagaaaca agcaaattaa tgagttgtcg ccagctagtg 7680

tatatatcca acagtaaatt actagatgct ttagaatgga aagagactag agatagtcat 7740

ctgtaattta aatctaaacc acacatgatg atgtgcttca gaggacacgt acgttctgaa 7800

aataaacatt ttccggagta agtgtatttt ttaaagcgta gtactatagt ctggtcaagg 7860

gatggcagtc gggcacgacg gacacgtacg ggtagtgact atcaatgcat gctcgtgccc 7920

gcgaggtaat ttatgcctgt gggcatgccc attactacat gacagacaga caaggaacag 7980

tgtccacgcc tgtcgcccgc gagcacatgg tgcccgcggg tatgcccgtt tacccatcat 8040

agactcatag ttcaacactt caaccacaaa tctccataaa catagaatca ttatcacagt 8100

ttaaaaaaat ggtgcagtta tatcaagttt aacgatacaa gcattacaag ttcatcaatc 8160

acaaatgaca tagttcgaca taatgataga atcaaacaca catatgagca taattagaca 8220

taatagatca aaatcgggag ggacaagatc gaaaaaataa acaactagtt ctagagtagg 8280

aagagaaggg gaatatcttg ctcaagcaag gctggaggaa ggtgtggcca tcgacgactg 8340

gagggtgtgg cggctgacgg caggaggacg tcgacggctg gagggggagc tcggcgcagt 8400

ggaggtagga ggtggcgacg accggaggtg ggaggcagcc gaggtggacg aaggaggtgg 8460

ccgacggcgg cgatcgtagc ctgcacggct agctgcctct cccgtggtgg ctgtgaaagt 8520

caccggggac gaccggggtg ggaggcggag ggatttcggc agccggcgac gaccgggggt 8580

gggaggagga gcaatttcta cggccggtga cgaccggggg agggaggaga aacgatttca 8640

gcggccggcg atggctgggg gtgggaggca gaaggatttg ggcggtggcg acttggagga 8700

accgaggaaa ggaggaagtc aggtgtggac tgtggcggtt gtggaggaaa gggggtggcg 8760

gctatggagg aaaggggtag ggaagaaaaa accctagaat cttagatggt ggtatttata 8820

cttagttgac cgagagccca catgtcagtt ggtttttgtg ggtaaacggg cgaagcgggc 8880

atgggtagtg actacccatg cccgtacctg tatgaccgtt gggtacaggt tttgacccaa 8940

taaaatacct atggatacta aatggaaaac aagccaactc tattagggtt tttacccacg 9000

gataaacagg caaacgggtt aaattgccat ccctagtctg gtcgatttga accacatact 9060

gtgatttttt tttcacatag cgaaacttta ctcaaaaaat tcaaggttac aaaaaacata 9120

tgtattattt gacaattaga ccagggtgac tgcattatat atttatttat ttgaaaattg 9180

agtacgtcac ttaagtactg tttgtaaaat tttcataaac aatacttaaa agacgatttt 9240

ccattacata gaccttttag attgcagacg gaccgtaact agtcacgtct ggaaaaatcg 9300

tcctcccatt tttgcgtgcg gatccttaag aggatcacac gtgaaaattg attttcgcaa 9360

gtgggcctct taaggatccg cacgcaaaaa taagcctatt tttgcaggcg gacatcttaa 9420

gagggtgcac gcaaaaattg atttcgcatg cgggcgtctt aagaccaccg cacgtgaaaa 9480

taccctccct catttttccc tcacaaaact tcaaaaattc catcttatgt ccttgctcca 9540

actctctcac ctccttatct ttatcctcac ctccaaatct ctctcctctc ctctcacgca 9600

ctcacacggc agtaggcggg cggcctgaca gcgggcgacg gcgggaggag agggccctga 9660

ccgccccgtc gccgccgcca acgatgacga ggtggccagg agcgacgacg acgagttgcg 9720

gcggcgacga cgatgacaat gatggtggcg acgacgacag cgacggcgac ggcagcgacg 9780

gctgcgatga tgagaaggag gtggctggag cggaggcgga cggcggccgt ccccacgctc 9840

tccctatgac ggtgctgccc tctccggccc tcccctgcgc ggatccggtg gtggagcccc 9900

tctcccgccc tccgccgccc agatccggcg acgacgaggc acggcgacgg caacagcgac 9960

ggcagcgacg gctacgacaa cgacgagctg aggcagcaac tagggttttg atttttcttc 10020

gtgatttttt ttggaatttt attttcacgt gcaggcggca taagcacccg cacgcgaaaa 10080

tccaattttc atgtgtaggt gcgccacccg cacagataaa ccctgatgtt tgcagagact 10140

tccgggttga cggatgccac ctctgcacgt aaaaactgtt tttgcccgca caggaaaatt 10200

gttttcgtag cagtgaaaag aggtgtaaaa aagcacgcaa cgcatgcata tcaaataatt 10260

cgtcgaatac ggaacaaatt ggaatcgcta gctatagtct ctgttcacca ccaaaaagaa 10320

tcccacaaaa aaataattct taacccttct tgtaggagta ctccatatat atacgtaatg 10380

ccgtggctta atttactatt gcaccgtaat cttgtgatca aggacttccc cttatttggt 10440

taattaggtg aagcaccaac tgactaaaag tgtatatata gttgcgtatt tagtcgtccc 10500

acaaactttc caattttcca agatgcgggt gcatatatat atatatatat taaattatat 10560

acttatatat atatataaat atataattta atatatatat atatatatat atatatatat 10620

atatatatat atatctagtg taaatcaact tcgtaattaa gcataactgt ccacttgtac 10680

tttctgcaaa atgtgataca tcctctgtag caggtgtgga ccaataagaa ccacactatc 10740

aaaagcagtt ctgaaaatat atttattccc cctctttcca aaaaaaaata aaacagtgga 10800

tgaatcatgg gcgcaacatg gtaataatat aaaaatgatt gttaacacat aataattcca 10860

acaatgtgaa gaacaatttt gtctttaaca accttttttc tttttaaaaa agcacatgtt 10920

tagcgattta tttctagaaa ccccctaagg gtatgttcgg aactgctggt tcccagcttc 10980

tccgcctcct tttccgcacg catgtttttc aaactgttaa acggtgcgtt ttttaccaaa 11040

agtttatata cgaaagttgc ttaaaaaatc atattgatct atttttttta aaaaataggt 11100

aatacttaat taatcacgcg ctaatagacc gctccgtttt ccgtgcgtgt gagatgggtt 11160

cccaacaccc acgaacaaac acagcctaag agttcggcaa aaatttcttc tgttgaactt 11220

catgaaaaaa aaatgtttcc tacaaaaggt cattgcacta caaataaagt gagatgacag 11280

aagcttgatg tttcagatag ctgatttagt caaatattta agcggttcgg ttgtacgtcc 11340

aacctctacc tgaattaaaa ttctagattt agatccagca cccatcaatt tgataagtac 11400

acacatgctt gtccatactc gtgcccgcgg gtgtttgctg cctttgataa tatcccagtt 11460

tgcgtagaga atacacacac gtatatgggt tagtgtgtgc atcaaatgca ttctcttaaa 11520

aagaaagaaa taattgagat attttaaaaa gcctaaggta catttcttgt ctaaattaat 11580

accattatga ataatatata cttcatacaa atgttcgaaa ccaacattcg cacgaagtat 11640

ccttacgaaa aatgtttgaa acctgaaatt tctcagaatg atataatagt taaaaatata 11700

atatggcagc ttctcaacat attattcaaa gaaccactac cccaagataa cttgaatttt 11760

aatattagat gagttatata gaaagaccag tttgtgttga catgacatgt ggctgctgag 11820

tagttacatg cagacaatcc aatccatcct ttgggggtac aatgagggag ccccaccatt 11880

cattgttgca atcatgctag ctactatagc attttccacc tccaacacca cccgttcaca 11940

tgagccacgc aaaaaaaagg ctattccccc tcaccctctc ccgtttgcac caccaccaat 12000

ctctctctct ctctctctct ctctctctct ctaagatctc tatctatcta aagatctgag 12060

ctacaattaa ttaaaacctg ataagaagaa gaccctgagc tcaatccctt cttattatac 12120

tatatatata cttctgtatt tgtgcacagt gttggtacag gtatctgcag acagccatat 12180

ctatctttct agctaattgg caatatgcct gaagtccact gctcgtccta agatttactt 12240

ctcccccccc cccccccctc ctttgcttct tcttcatcat catcttcttc ctcctcctgt 12300

taattaagtt cttgatttct aagctagcaa ttgagcacac atcgatcgcg agagacgcag 12360

cggcgcccgt ctaccgcggg gctagaggaa tgtcggcaca gatcgtgccg gcgccggagc 12420

atgtgtgcta cgtgcactgc aacttctgca acacaattct cgcggtaatt tgtctgaatt 12480

gttgatccct ttctccttct ctctctctct ctccccttcc ttgatttcct tgcctcctct 12540

tttctacatg aagtttcttt tcttttttgg gggggaactc tcgatgaagg tgcagatcta 12600

caagggcaag ggagatcgat gcatatatga gatgattggt tgcttggctt ttctttagaa 12660

ttgtgtatga tttgggcagt tcatttgcgt gatttcttta atttttaatt tgtgcgaaaa 12720

gatgtgtgtc atgctgccaa gagattgggt ttcgatatat atgttacttt ttttttaccc 12780

aattaccctt cgttttcttt ctttaattgt cgacagagtt cagatagctt cacctccttt 12840

ccttttcgac tcattaaaac tctggtccgg ttttctctga aagagagctt gtcgacccta 12900

atttttttga tttagtctat catggagtca tgggcatgat tacatatgaa tgggaaaaag 12960

ggcaaaggtc atgatgatct tgagaattga gctgcttctc tctctttgca ccattttgtg 13020

tcgtttgatt atatgggttc atgaattatt tgggcgagtt gatgaacttc aattaactgg 13080

tacgtactag agtagctagc tctagatcat ccttgttttc tctctcgact cttaatttcc 13140

ttcagaacct agcgtagctg atgctagatc gatcttacgt acatatgaag tgcagttgat 13200

tttcttgccg gtcgactggt actttatatg gctaccatat ataagagtat cgtcgtttca 13260

ggtgttatta ctagtaatcg aattgctaca aattaaagag catgcaataa taacacataa 13320

ctttaattag tatatgcaac tactgtgtgt aatgttctcc tgcttgcctc ttagatcgat 13380

catcttttaa aatccacttg ctcatcccct ctaagcacaa atacagcatc tgaaggactc 13440

agaaacggca acctctcgaa gtacggtact gtgttacaag aattaaggtg caaattgcat 13500

caataattcc tgtatgtatg tgttcccgtg ctagctgttc cactcattcg ttcttcccgt 13560

gctatatctt ggctttcttg atggtttctc tttctttttt ttcttttctt tttggtgcaa 13620

tgcatggtgt gagtttaagg actaagtaca aataactaaa actacataat taaaatgaag 13680

cagaagcagc tagtaaccat aaggcaaaaa gattgttacg tccttccaaa gaaaaaaaat 13740

tgtggtttga gcttgagcta gcttcttctc cttcctcctc ttcttttttt ccagagagag 13800

agagagagag agagagagag agatgcttga gatggcatgg ccggctagct tcactcaata 13860

catgcaaggg aattagatcg gtccttatat atagactata gtcatcagtg atgtgttgaa 13920

ctggtctgaa tggcgtttta actagctaat ccccatgttg aactcatata tacactatca 13980

ggaattcgta taaacatttt aatttctctc tctatttttt tttctgtttt cccacttctt 14040

cttggatctt gccttgcttg ctttggaagt acacgctgct agactagtga tatgatatga 14100

tcgatccccc tcagtcagtc agtctgatct gcatatatgg gcggcgagag tgctacgaaa 14160

tatcgcaaat tcgtgcacac acacaacata acaaatctct agctagtttt ctacggttct 14220

caatgaattg aagtatatat atctttatat atagtgcata tcaattattc ttgttgatta 14280

attagttagt tccgttcgat tctcgacatg ttaacttctt ggcacaatta catgcataca 14340

gtctttatat aggagtatat atatcttgtt gatctgcgct acttgcatgc ttcaatatat 14400

ctttcagact ttcagaagag aaattatatt ccttatctct gaagtctaaa tagctactgt 14460

agttcgcaag aactttattg gaggccatct tttactccat ctcaatctct cctactccct 14520

ccagaaagat aatcaaaata agcgcatttg ggaacaaata tagctagtga ccattaatga 14580

gagtcgggcc caaacaattc aagtattgta aataccataa cagcaaaaag tagaatacac 14640

caaaatgagt ctattaccag ttccaggaag agcacaccaa atgacagaaa ctattactca 14700

atcccaaaac aaattaattt ttactatagc taattagcta tgaacttgga aagtagtgac 14760

cgggatatag agagtgtaca cgccaacgac aaatgcagag ccgtacgtac tgacttcctg 14820

catctacatc acccactgta atgtcaattg ctgaggcgta gtacacatgt ctagtgtaga 14880

tagtacatgt atagctacat atgcatccat gcatgatctt tcacaagatc ttaccagcat 14940

gtctcaactt tactactgca actccaatac ttatatatgt atgtcttctt gctctctcct 15000

accgaaagat gaaaaaaagg ggattgaatc atcttgtgca ttgcatgacc cacacttaaa 15060

tgtactccct ccatccaaaa aaaactcaac cacccctcct aagacaacac ccctcctaag 15120

acaacgaatc tgaacaaaag gtagtctaaa ttcattgtcc acatcctctt ttaggttgag 15180

ttttttttgg atggagggag tacccacaca aacacacatg caggagagga gatgttgtgc 15240

ttgagagtga attggcttct gcatgaaggg tcactgtctc tagctagcac ctttctttcc 15300

tgcactgttc atgcatgtag ctgaaggctt tgtgatttcc tactactgct actactactc 15360

tgtggagagg ccagacagag aggcatgaca tgacatacca tgcaaatgca tggcttcttc 15420

ctttaaaaaa gtcaccaata gatatgttgt gaggttgttt gcttctgtca caaccaacta 15480

ctagcagcta gcccttttgt gctagatatg tgtggagtga agtgtgattt gagctaactc 15540

ccctcatggg agcacacaca atcattctca ttcagtcact ttgcttaatt atttctttct 15600

tctttcttat gggagtgagt gcccaaaaag tgagtgaatg tagggaatca aacaaagtag 15660

aggtactgtg ctcttcaggt ttgggaagaa aaatgggaac ggcaagggac caatgtgtgc 15720

aggcattgga aaagggtgcc ccaattctga tctgatcaga tatatattca gatcagcaga 15780

gcccctgata aaagtcatgg aggaacagtt atccacatct aaagctatag ctgtgtatta 15840

atgtgtatct gcagtctaca ccagccttat gaaacatgca tgcatgcatg catgcctttg 15900

agatttgatt ttttacttct cagattcttt tgtacaaatg caaaaagaaa atgatccttt 15960

ggaatctagc atttgttaca tatttacaat ataaagtgct atacttgttt acttttcatt 16020

ctacttgttc cagcacatgc atatgtaatt gtacacctgc tcaaatttac aatgagtggc 16080

atatatatta tacatattaa ttacggctta cttgtatttc ttccataaaa gaacatgtct 16140

agaatatttc ttttcttgac agtaaatatt caggacatat tatactgatt cttggaatca 16200

atctacaggt cagtgttcca agcaatagca tgctaaacat cgtgaccgtc cgttgtggcc 16260

attgcactag cctgttgtca gtgaacttgc gaggattggt ccaagcattc ccagcagaag 16320

atcatttaca ggttgacaca tcaatatata ctactctttc atgcatgaaa ttatggatac 16380

agatacaatg actacattaa atcatattat ctctttttaa taaaaaaaca cacatatgtt 16440

gcctatatat atctagaatc caaattaatg gcacttttca attataacca ttgctactgc 16500

atgcttagtc tgatagagct agaaatcaac gaacaattaa tgtatatgta ccggagcatt 16560

cttcacatac ccagctggaa aaaaatcaca tgcagatcga tgtatgttaa actaccctat 16620

atatgtagaa gtagtatagt actactagta tatgtagata actactccct ccgtttcaca 16680

atgtaagtca ttctagcatt tcccacattt atactgatat taataaatct agacatatat 16740

atctatctag attcattaac attaatataa atgtgagaaa tgctagaatg acttatattg 16800

tgaaacggag gaagtagatt ttactcacag agaaaaacag aaaagacacc atgcaatgca 16860

tgcatgggtc atctcaagct tgaatacttt acatgtacag cagagacaaa ttaaagcatt 16920

gcactattta ctcacaggaa agaattttaa agctcttgca catgccaaaa tatatactag 16980

gagtaccaag ccatatggct agatcagcta ggtagctcag gttgctcagc tgatcttata 17040

ctacatgact tgtgcccctt tgtcaaaccc tgcagggtta ttccccgcaa aaaaaaaaat 17100

cctgtagggt tattgataaa tactagtaga tgttttgcat ttgtttttat atgctactat 17160

atataatgga aaaaatcctg ccacacaagt acttgaatta aatttccaat atgaaaagca 17220

cgagtttcga attccggcaa taactacatg ttaggtttga tatactccct ccgtcagtaa 17280

ggtgtgttta gttcacgcta aaattggaag tttgattgaa attgaaaaga tgtgacggaa 17340

aagatggaag tttgtgtgtg taggaaattt ttgatgtgat ggaaaagttg gaagtttgaa 17400

aaaaaagttt ggaactaaac tcggcctaaa tatgagggat acgttactgt agaatgtaca 17460

aaattgattc gccaaattca tgtttagaga ttttcatgtt tgcttcacaa aacaatcaaa 17520

agccatgcag atttttcgaa atgatctgaa cagtaaccac caatgtttta cttaattttc 17580

ttgtcttgac ttggttataa tttccatgta aatacacact aagtagcatc tagaggaaaa 17640

atgtgtgact ttaagttgat taacttgctt cttacatttt cctcaggaca atttgaagat 17700

gcacaatatg agcttccgcg aaaactactc tgaatacggc tcatcttcca gatatggccg 17760

agtgccaatg atgttctcaa aaaatgacac agagcatatg ctgcacgtgc gacgtaagac 17820

aatgcatatg aacctgcata ataaatttag tgtacatgtg cgacggagcc atatttctag 17880

agattattga ttttactata tgaagcaata taaaggtata caaatgctag gggaatattg 17940

catatgttca catatacatg ctgggtgcat ataccacttt tcttctctaa acccttttta 18000

tctttgttgt tttgctcaga caactggggt acgtgtgtta cttcttaatt aacacaaata 18060

catgtataga gaaattatac tggcttaatc aattcctgag ttatgcactt ttttaaacag 18120

atatatttag aaaattcgac gagggataaa cctctactaa aaagtcagaa aaatagaaaa 18180

tttaaacaag ttgcattgat tcagctagag gtggataata aactagctag gctcacaagc 18240

tggcttgtta agctcgagtt ttatatcaat ttaacatgta ttaatctatt atatgttcac 18300

ataatagtaa acactactcc ctctgtttca tattaaagtc gtttcatatt ataagttatt 18360

ttgattttat aaaaaaaagt cgttttgatt gttttcctag ctagtcaaag taatttaagt 18420

atgaccaagt ttacagaaaa atataacatt tctaatacag aacaaacata ttaaaacata 18480

ttcaatgcta gatttaatat aactaatttg gtgttttaag tgttgcaaaa ggtttctata 18540

aatttaatca aaactttaaa aattttgact agaaaaagtc aaaatgactt attatgtgaa 18600

acggaggaag agtaactagt aaagtactaa ctagacatga atagtttgaa agtaatttca 18660

agaagtatta tccatagtat aagtattgac taataggcac tcggtatata tggaaaatat 18720

ggtgtgtcta gttaagctaa caagctaaaa atccatctca caagttcgag tcgatcagcc 18780

aagccacaag ctttgagttt ttttccccaa ccctagctag gcttagtaaa gcttgttcca 18840

cccaagatgc gcgtctgctg ttattgatca cgttaagttc tgttaaagtt tatggtcccc 18900

atcgcttggc ttatttccta ataagccaaa acggcttatt agaaaataaa aatgaatttg 18960

taggcaaaac ttttataatt gtgttcttgg tgacttaaaa gccaatgttg aaaagaaact 19020

acgttgaaaa tatctcaaaa tcaatctcaa aattaagctt gaaaatttaa aatttggctt 19080

tttctttggt tgattaggcc atccgatggg agcctatact gggctgtata tagaaattat 19140

tcttatatga attaatgaat gaaacgagat gtgcatgtgt gcgtgagttc tagctagtag 19200

ctagtaccat gcatggttcg gttaaagatt tttccgtgcg gtcaaaatac atttttcagt 19260

gcggaggtcc cacccgtctg taccctgcat gtctgcgaaa atccatattt tcgcgtgcgg 19320

gcggcctaac cgcatgagat aatccatctt cccgtgcggt cgggttaagt agaccacacg 19380

ggataatcga ttatcccatg cggtcaaatt ataccgcccg cacgcaaaaa aaaactgaaa 19440

aaaaataaaa tcaaaaacac taaccctaac cctagaaatc tagaatcgcc cgctgccgct 19500

gctcgtgttc gccatcgtcc tcatgtcgtc gtcgtggtcg cgccgtcgcc gctctcatca 19560

tcgtcgttgt ggtcgcgctg ccgccgctcc cggccgccgt cactgctccc ggttgccagc 19620

gcggcgcgga tccgaccggt cacccgccgc gccgtctccg ccagcagccg tcgtgccgcc 19680

cgctcccggc cgccgcctcg ttggcggcaa gccgggcaac tcccgcgccc gtgcgctcgg 19740

tggggagggg aggagctaga ggaaaggcag agagagagag aggagtgatt gagggtgaat 19800

aaaagggaaa aggaatagag aagatgaaaa gtttaaagtg agtaagagag aaaagaggga 19860

gaggtatttt cgcgtgcagt ccacttaaga ggcccgcacg cgaattcgcg tgcgggcctc 19920

ttaagggtca gcacgcaaaa ataggcttat ttttgcgtgc ggaccgttaa gaggcttgcc 19980

tgcgaaaatc gatatttgcg tgcaggtctc ttaagagtcc gcacgcgaaa atgggaggcc 20040

aatttttgca gacgcgcgcg aaaacggtcc gtctgggaaa atggaagctc cttgtccaga 20100

aaaatgtttt gtgtactagt atatatttga aaatactgat gcatgaacta gctacttgag 20160

tactatttca ttttgtgagc ccaaaaatat tgacgtacga actacttgac tactacgtac 20220

tcctattaat ttcttgaaaa acctaggagt agtatacata ctctcaagag tatgtgcttt 20280

agatagtaat taaacagtat ctggatgctt atactttttt tttacaaatc tttttcatac 20340

aacttgcagc tccagagaag aggcagcggg ttccttcagc ttataacaga tttatcaagt 20400

gagtttgatc tcgagacaat tcgatcgatc catatatata tatatagatg atctagacag 20460

cttataacat ctgagagttt tcagataatt gatataaatc tgccgtattc tatactaaga 20520

gagtgtttga ttttgacacc cactaatata tcactacagg gaagagatcc ggaggataaa 20580

agcaaacaat cctgacataa gccacagaga agccttcagt actgcagcaa agaatgtgag 20640

tacatgtgta gctaggggaa acaaattact atcttaaact tttacatata ctgatataat 20700

agctgttctt ttttttacta caaactataa tgcaaattct cacacacttt ggcctggttt 20760

agttcccaac tttttcttca aacttctaac ttttccatca catcgtccca attttaacca 20820

aacttctaat tttagcgtga actaaatata cccttgatca ttgctgaata tatattgata 20880

gaagtatgtg agccatatac atagagttac atatcaacaa tactacatat tgttgacttt 20940

gtatgtcact gctagcaaga tattaaatcc atggcaattg ccatttgctt aatactctta 21000

tacatatagt agtgtccata ttgatcacta aatactcagt ctatatgaat ttaatttata 21060

taacatattc gtaaaaaaaa tagtggtcaa agttatatat ttaagaccat gtcatagtct 21120

aaaccatcat ctacttctga ctagagggag taactctcat ctcatatgca tcctactttt 21180

tttttttttt tgcagtgggc gcatttcccg aacatccatt tcgggttagg ctcccatgag 21240

agcagcaaga agctcgatga ggccattgga gctcctagtc cccaaaaggt tcaaaggctg 21300

tattgatcga gtacacatga tgatcgatgg atcctacgtg cttgttgatc tcatgttgta 21360

agtaacttaa ttaagaaaaa aagtctacgc ttatttattt cttaagtatc atcagttaaa 21420

tttgtgagcg agtatataga tgagttgtca tgatcagtga gtgatcgtat gcatgcagtg 21480

ctgagaatta aaggtgtata tgcatgagta acgttacatg cagctagtgt cgacgcacgt 21540

atgacagact gaataaactc ttcaccatgt tgttttttta tgtttgaaat gaattaatta 21600

attgtccatg attgcaatcc atttcatacc ggtattattg gccagtgcag atcaaagttt 21660

ctacagctta taagtactat atattgtatc gtatatgtgt gcacgatgca ctaatccatt 21720

gtcatttgtc tacacggtac gcaagatcga tcatactgtg tacacatgca tactgtgcat 21780

agtgctgttc ttttgtgcta aatactaaac atatgaattg tcaatcagta atgtactact 21840

atattatata tataatacta tatgtatgta gattttaatg aaagattgag cttgcattta 21900

tcctgcaatt gagaaacatt ggaccaggtg aaatggtact ccttccgttc cattttaagt 21960

gcaaccataa gtttccgtgc ccaactttaa tcgtccgtct tatttaaaat tttagacttt 22020

tttttcacat cattctcagt tactttgaca ctttgctaac actatatata attatattga 22080

tgagtaaaac agtaccaata tacaaaatat attaagaatt atgtaactat atagtacctt 22140

tttacattct aacaacgtta ttttttactt ttatttgtag ttttaattga tgttgtctcc 22200

aaatatttcg agccagatgt gtagatatat atacccttag gctatctcca acaatgaaac 22260

ctaaaataca agacccattt cacgtttgca tagcgctaca gtcaaagagt tcaataccta 22320

tttttggtct tctccaataa taagacctaa aagagaaccc ttactgcaaa taggtcttca 22380

gaagagagga tactcagatt taggttatgt ctcttatagc acgtaaaata ggtcttctgt 22440

ataggtactc tgttgaaggc tgaaatagta ctatcggaga cccattttgg atttgggtgc 22500

ctttatgggt cacttgttgg agacagtctt atacaaacat actggagtaa atatgcatat 22560

ggatatattc acattgaatt cttctgcagc tagttagcta gctacacaca cataatgaca 22620

ttatatagtg cactgtgtgg actgtggcga tggcatgcat cgatcggcca cgcgcgcgta 22680

ccgagtaatc ggcgtatgat cgcatgcatg cgatcatcct gccatgcatg cattcattgg 22740

acagggccac tagtcacgat gcagcagctg ctagctgcct ccccatctat gtgctggaga 22800

aattactcct actacagctt ctccatgcat ggaatctcga tcataatttg ctttgctttg 22860

tactgctccc tccatttcaa attgatctac atataatttt tctaaggtta ttcctaaatg 22920

atctacatat ttatgttcat ttattaagtc tattcgttat ttgtgcattg gagtaaattg 22980

acattgatgc atgtatccat gcacacaagt atttatagcc cacatgcaat atcttgatat 23040

ttgctattgg ttaggaaata gtggggatgt tgcatgcatc gagtttgttg ctagagtaaa 23100

tatagtatga gagagttatt agcttttctt ggtcttgatg tacctatgaa atatgtagat 23160

caatttggaa tggagggagt actacactag ctgctgtgct gattatcatg agctcagcat 23220

tgcagattca tcactgatga tttactgact tttggtctct ctctctgcta gctctctctg 23280

tatgtggtgc atatatttca attaatctgg ctccaatttg gtcgtttcga cccagtacag 23340

cacgtaccag catttgtttt cttgctggta tgctggtagt actagctaga gctggtggcc 23400

agcctgagac agaatgacaa tggctcatat agaattgaat ggcctgcaga gcaacatact 23460

actactatat accggtcagt gttccaatta tgggacagta taaaagtttg ggccaccaaa 23520

agttctgagg ggctgtttag tttccaaaac aaaaattttt acctatcaca ttaaatattt 23580

tacatatgta tgaagtatta aatgtggaca aaataaaaaa ccaattacac agtttgcgtg 23640

taaattgcga gacgaatctt ttaagcctaa ttgcaccggc catgatttga caatgtggtg 23700

ctacagtaaa catttgctaa tgacggatca attaggctta ataaattcgt gtcgcagttt 23760

cctagcggaa tctgtaattt gttttgttat tattagacaa cgtttaatac ttcaaatgtg 23820

tgtccgtata ttcggtatga caaccaaacc taaaattttt cctcaactaa acaaggtctg 23880

aattaccacg tccatccgaa aattaatata ctggaatagc tatttctggc tgtgaaatac 23940

acactccatc caaaaatata ttcatacttc atgtaacaaa gacacgaaac tatgtgcatg 24000

caagtaacac tggtggagaa accatctttg ctcggttagc caaaatccac aatattcccg 24060

gttgcaataa aaaccgggat taaagattat tgttagtccc ggttcaaaag tttagagctg 24120

ctttttaaac caaccgggac taaagatcta tttttaatcc cggtcggttg gtgttaccaa 24180

ccgggactaa agatctattt ttagtcccgg ttggtaatac caaccggaac aaaagatcgt 24240

ggcggagagt atgaggcatc tgccatctgc gagatcgagg aggaggagac gggcagcctt 24300

atctcttcac ctcttcctcc actgacctta tctcttcatc ccatctcttc ctcttcctcc 24360

tcctcctctc ctctaactcc tctccttccc tcccccatca tcccttcctc ttcctcctct 24420

ctaacttctc ctctaactcc tctccttccc tccccatcat cccatcgggc ggccggcggc 24480

ctcgcggcgg cgcgccggcg ggcgaaggcc gggcagtggc ctcgcggcgg cggcggcgct 24540

gcggcgcgac ggcgcggcct cgcggcggcg cggcagggcg gaggccgggc ggcggcctca 24600

cagcggtggc ggcgctgtgg cgcggcggcg cggcggcaga ctgcagggcc tcgcggcggg 24660

cggcgcgccg tcggccggcg cggcagcggc caacaacctg tgtttctttt tctttttttt 24720

taagagtttg tgattctgat ctatgtattt gatttgtgtg tgatgtccgt gaatatgtga 24780

tgtatttgtg atgaattttg tagaagttgt gatgtaattt tgttttaaga ttttgtgatc 24840

tatttgaata tggccgattt ggggatttga ggattgggga taatttattt gggattttgg 24900

gatggggata gaataaaatc aatatattaa aaacacaatg aagagaagaa aataataata 24960

gagcaatcgg atctagctag ctaggctagc tctatcttta ccaaccggga ctaaaaagtc 25020

tctatcttta gtcccggttg gtaacaccaa ccgggactaa agatgtattt ttactcccgg 25080

ttttttcacc cgggactaaa gatagtgatc tttagtcccg gattggtagt cccggtttaa 25140

aaaccgagac tacagggagg ttacgaaccg ggagtaaagc ccctttctcc accagtgtaa 25200

ctagagtact gagatggatg atcgggttac agttaacaat ttaatttagc accgtaaaat 25260

aaaaagataa ctccttcatt taagtcttag atgcatggag actacaaata taccatctca 25320

tttggaaaat aaaaactaga aaatgaaata gtctcaaggc ttttggacgg agggagtaca 25380

agctaaaaaa acaactatat tttaggacag aggtagctta ctcccttcat ctcaaaatat 25440

aaacatttct agctataaca gctgcttgtt caaattcata gtaaatactc cctccgtctc 25500

ataatataag ggattttaaa tttttgcttg taatgtttga ccactcgtct tattcaaatt 25560

tttttaaaat tattatttat tttatttgtg acttacttta ttatctacgg tactttaagc 25620

acaacttttc ggcttttata tttgcaaaaa aaaaattgaa taagacgagt ggtcaaacgt 25680

tgcaaacaaa aactcaaaat cccttatatt gtgggacgga gggagtagtt actatatttt 25740

ggaacaaata tagtaatgag ttattcaaaa tataatagaa ggttgtataa acattattgt 25800

cattccttcc atttaaaaaa ttattgtaaa tctctattac tataaaaggg caaccatcct 25860

ccttccatcc tctcttctta ttgtgtgcag cgcatctccg tttgcataac tactacatcc 25920

aatattcctc aatcgccctt ctagctagcc catccctaat tattaggttt agcccgttac 25980

aatgtattaa taaaaaaata aatatagatc ttatgcttaa gatacgttta atgttaaaac 26040

aagtcgatca caacaaaata aatctttctt aaatagttct tttagcaaaa caagtgatca 26100

aacgttatat ttgaaactta acatcacata ttttttttta aaaaaagagg tggccgcgac 26160

tgtcgtaaca gtattcatct cctagcctgt cgtacagcgt ttaaattctc tatgaccctg 26220

ttgggggcac cgaaaaattt tgcatttata gatcgatccg ctagcaattt ttggtgcccc 26280

aagtttactg gtgaaattaa caaaaacaca cacacacaca catgcactgt agggagaaaa 26340

ttgacattgt gccactttga agagtgggtt tcgctataat gccactccgc aaagtggctt 26400

tgttaaaatg ccatcgccaa cgactgccaa cacgctgctt cgcatttttc cattttcacc 26460

ggattaaggc acgaattcgc ttgtgttctg accaatgtgc ccctgactca tccacggcga 26520

tgacgtccgc cgccgtggac aggtcgtggt gctcaacggt ggcgggagtg agggtgaagt 26580

gttgtgcggg cgccatcacg atgggcgtga tgtttgcaag gtggggagca ggtgatgaga 26640

aggtgtacga cgtcgcggag tgatggctgg caccggactt cccggtctgc gcatgcgcga 26700

cagtggcggc gaagccaatg tagtcggcga tggccgcggc aacgttcatc gactgccacg 26760

cctgctattg cttgtggcca ataggtgagg cgttggctgc ggccatccca gtgagtagct 26820

cggtgaagaa agccgcctac gaggaggcgg gagcgggtgc ggtggtggtg gcggcggtgt 26880

ccccggcgtc cttggatgtt gcagcagctg cgctactccg ctccctcacg cgcttcctgg 26940

cattgacgcg gctctcggag cgggagagcg agaggacccc cgcgggcgtc atccaggggc 27000

cacgcgaagt agtccccgtc ctccaccacc aggtctccct cgcctcctcc gccgccctct 27060

ctggctccga tcatgacctc gccttccacc tccagctcct gcaccctggc gacctcacgc 27120

gagtcgagca ggactgccga aacacccaag cctaccacgc ctaccacgcc cgagccgccg 27180

catccgtccg tgtcgccgtg catgacgccc tcttcgtgcg tgacctcgcc gccatcccag 27240

aggataggta ggcccacgac ggcgactact ttgagcggcc cctggacaac gcccgtggag 27300

gaggcggtgt gctcttccgc gtgttggcgc ggtcggcggt ggccggcggg agggtgaagg 27360

gaccaaaggg tattttggtc agtcggggtg caaaaaaatg gtgaaaatgg tgtgaattgg 27420

agattatggc aaaagaaagg tttgagatcg cttagaagtg gcattttaac gaaaccactt 27480

tgcggagtgg cattacagcg aaacccacga ttcaaagtgg cacaatgtca attttctcat 27540

gcaaacacga tttaagcaac atcggttcag cgtgtcaatg acagcatcca agttttagat 27600

cgaaacagca aactgaagct aaaaagcaag caacaaactc tgcacattca tggcactcaa 27660

ccgatcgata gtacattaat cgtgcatacc agttgcaaat cgatttacac aaatcaatga 27720

atcattcgca tttgaaccaa atcatcaagg agcatgcatt atactccctc cgtcccaaaa 27780

tatttggcgc tgttgacttt ttaaaaaatg tttgaccgtt cgtcttattc aaaaaaattt 27840

aagtaattat taattctttt cctatcattt gatttattgt taaaatatac ttttatgtat 27900

acatatagtt ttacacattt cataaaagtt tttaaataag acgaacggtc aaacatgtta 27960

aaaaaagtca acgacatcaa aacatttagg gaatgaggga gtatattaac tttgtgatcg 28020

attcaagtat agatatatag aggatgagat ggaggagaac caatgcagag gtttacagtg 28080

aagggcatta gcccatccta tatagtatcc accgttgttt taaatattaa tggactagga 28140

tttggtgcta cagtaacaac gagcacaatc aaaatcgaca cagtaataat tcccaaatag 28200

taaaacaacc attattgctg cagtgttttc gtcacagcta gcactagtgc ttgttactgt 28260

tcacacggag tcagattaat tactgcttaa acactcccca tctctctctg cattactgca 28320

gatgatgggg atccggggaa taggagagcg actttgtcac agctgacaca gtgaccccca 28380

ggaagggcaa ctagctagga cgaccctcga cgtcgacggt gtatatatcg accgaggcag 28440

gcaggcaggg gatcaatgca gtcgacgctg tgcatcgtgg ctgtggaatt agtgtggatg 28500

gaggccgggc aagcgcggtg gcggggtcag acaccctcag gctagaagcg aggccatccc 28560

gcgccggccg ctgctggatg gggagatcac cggagttggg gagaggagaa ggtggcgccg 28620

ccgctggact ctcggaggac cacacaattc aataagccca gtttagaaaa agatcatggg 28680

cggcccgtaa ttagttgctt cgatcagggc tgcctgcacg cgtgacagag gcgtttcggc 28740

ccattctaca aaccggctgc tggctcattt gtcatctcat agaatatata tataagtcta 28800

ctttgggttt tacgtctaat ttgattggca tccctaaata gtaataccat aaaggcttat 28860

tcggatcata agattgtaaa aatgtaggaa tagaaaaatc gaagaaatat aataggaacg 28920

aatgtgtaaa atagatgatt gaaaaacaaa ggaattatgt agataggatt agtaaatatg 28980

tgtttggata gacataggaa aaacatagga aatagaaaaa aggagatagg agtatatgga 29040

aatttttctt tgtctctcta ttgattccac atgaaaggaa tacttcctcc gattttcctt 29100

tgggtcgatt cctgtcaacc gaacggcatg aaaggaaata tttcctattt acaatattcc 29160

tttgaaaatc ctcaaaaaaa aaaatccctc gatccgaaga ggccctgaat ctttacacct 29220

tgacttacaa aaccaggcta aattaggtcc catgactgta tggatgactg gttttgctta 29280

catggcatcc aagtcagaaa aaagaaaata aaaaaaatgt gaagcccaca tgtaagtgag 29340

aagaaaaaat aaagaaaaat atgcggatgg cgctgatgac cttctccatc ccctggaccc 29400

tcgacgcgtc ccctcgaccc tatttgggtc accttcccat cgcccgagcc gatcccacgc 29460

cctctcaaca tcgttctatc atcgcggccg acatcccaca ccatcctcga cctcccacgt 29520

tgtctccaaa tgttataaaa catcgaggtg gtggaggatg agggtggtag ctagcggcga 29580

caggaagaga aggaggcaag ggtggaggag gaagccattg cagtgccttc tggccggcgt 29640

gggcgaacgg gattgaggtg gccgagctca ccgccattgg ggtgccgtct gcttttgcgg 29700

gaaagagagg gagagaagaa gggaggagag tgagaatgac atgtgggacc catttttttc 29760

cacataaacg cctagttaga acgagtggac cgggtcaact taccacgtca atgaagacag 29820

tcatctatac tatcggagga cctaattagt tttgtaagta ggcgtcctag atttttcgta 29880

ttacgattta ggaatgctaa ttaaaatcga cgtaaagata aagcagaaca agtgaactta 29940

ttcctcatct tatcgaaaaa gctactaggc ttatttggat attggggcta ttttgggctg 30000

taccactagt acagaagagc tttttccata cgggcgaaat ggatctttcc atgcggctag 30060

cctgcccgca tggaacaaaa ggtctgtaaa aatcgctatt tctccatgcg ggcaacacaa 30120

ccgcatgcaa aaaggggatt ttcgcatgcg gtccacatag ccaaccgcat gcaaagatat 30180

ttttcagcca aaaaaaataa aattccaaaa accccccgaa acactaaccc gccgcccgcc 30240

gccggacgcc gtgtgaccgg caccagatcc acgccgctgc ggccgcctgc cgtcgcgggg 30300

cagcgccgtg cccgccgctt gccgctgctc gccgtcgccg tcgccaagcg tcgtgctcct 30360

ccgtagggcg gcggatctgc cgccgtaggc cgtcgccgtg cccgccgctc gccaccgaga 30420

ccccaagggc ggcggatccg gccttcccga ggatggcgcc gctggatccg ccgctgtagg 30480

ccatcgccgt gcccgccgct ccccgccgag accccctagg gtggcggatc cggctttccc 30540

gaggacggcg ccgcctgatc cgccgccgct cccgccgtct caccgcaggg cgtcgccacc 30600

gcggctcctt gctaccgtcg cccgctccct cctcgcggcc gccgctacgc ctccttgctt 30660

ccgccgctgc tcccgccatc tcgccatcgc ccgctgcctc cttgatgtcg ccgcccgctc 30720

cctcctagct gtcgccgccg ccgctaccgc cgtctcacca tcgcccaccg cctcctgccg 30780

ccgcccaagt gagagagaga gagagagaga gaggacttca cggcggtgag gagggggagc 30840

ggcagtgagg agggatggcg gtggtgccgc cgcccgaggg agtcaccggc gacggaggga 30900

tggcggggga gtgagaaagt gagagagata gagagagacg agagatctac agagagagag 30960

aggagataat aagtgagagg agaggaagag aagataaggt ttggtgtgaa atttttgaac 31020

tggtagagag ggaaggaaga gggaccattt ttacatgcgg gccacttaac cacccgcatg 31080

caaaaattga ttttttgcat gtggcttttt agatttcaaa ttgattttta tgtcactata 31140

ttaactcata tgaaaaagtt accaaaaaca aagttgtaga actcatcaag atctacaact 31200

tttgttttga ccatttttct atatgacttt gtttgaatag tttgaaattg aatttcaaaa 31260

tatgacaact tcaaacaaaa ttttcaaata ataaatgatt tcaaatagaa atgtcatcaa 31320

caacaaagtg gtataactta tcaagatcta caacttttgt attggtcatt tttctatatg 31380

actttgtttc gacagtttga atttgaattt aaaattatga caatttcaaa caatattttc 31440

aaatactaaa tgatttcaac tgaaaaagtc atcaataaca aagtggtata actcatcaat 31500

atctataact tttattttgg tcgtttcttc atacgacaaa gttatgtgta acattgttca 31560

caaaatgtac atctcttata tagttttata aactatagag agagatatga aaatttgtga 31620

aaatgttacg atcactttgt cggatgaaaa aatgaccaaa atagaagtta tagatcttga 31680

tgagttatac aactttgttg ttgatgactt tttcaactga aatcgtttat tacttcaaaa 31740

tattatttga agttttgaaa ttcaaatttt taattgataa aacaaagtct caagaaaaaa 31800

atgaccaaaa taatagcagt aagaacacaa taacatgata gcgcatgatt ttagaaacat 31860

ttaggaaaaa taatcatcta atttggagtt cgaatgagtg ggatacacta gtttcaactt 31920

ttaaatttca aattttattt ttgcatgcgg gcgcttaagt gagccgcatg gaaaaatcgg 31980

tttttttcat atgggcgctt aagtgagctg catgcaaaaa tgagctcatt tttgcatacg 32040

gccctcttaa gaggcccgta tgggaaaatc gatttttcca tacgggccac ttaaggagcc 32100

gtatgggaaa atgaccctcg atttttgcag acgccactag ttatggtccg tttgcaaaaa 32160

tcatgggctc tcgtacagaa aaatcacttt tgtagtagtg tacgtaggaa aagatatcgc 32220

caacagataa gagacctcat agacaatccc agccacgaga ccagcatcca tatctcagaa 32280

aatggtcttt ttggacaagc acacacacgc ccgaacagtt ggtccaatca gaaactgtga 32340

atgatcgtgt gaattaacat tacaacataa ttagccatgt gggtggacaa ggtccttagt 32400

cgtgaaaagg gacatcgtac tctcgagaga gacattgcta gagtcttgga ggttgtctca 32460

tagagaatgg catagtttac aaatgtgtga attaacatta caacataatt agccatgtgg 32520

gtgaagaagg tccttagtcg tgaaaaggga catcgtactc tcgagagaga tattgctgga 32580

gtcatgaagg ttgcctcata gagaatggca tagtttataa acatgtgaat taacattaca 32640

acataattag ccatgtgggt gggcaaggtc cttagtcatg aaaaggaaca tcgtactctc 32700

gagagagaca ttgttggagt catggaggtt gtctcataga gaatggcata atggcatagt 32760

ttaccgattt gtataccaac caaatggccc ttcttgagtt gtgaccatca agtcagaaat 32820

aattaacaga attgctatat aacttccgat catgtgagac ggggatcgaa tcggtgacct 32880

tgtgggtgac agcatgcctc actagccaac aagccgccca catgttttta ataaatataa 32940

atacgggatg tttaaattag ttatatccta gctacaacca agttacaatg taataatact 33000

ccaattatat tataatcacg tttgaaatct tttcacctaa aaaattgtct aatattgtat 33060

atatatagtt ccaaaattaa ttaaaaagtg gatttcttta tatttctctt actggactaa 33120

ggttacactg gatcttaaat ataaattact ttaatattaa tagaattaat ggaattgtta 33180

accatcattc aacataaatt tggaaagaat tctatttgga gcattggaat gacgccaagc 33240

ttcatattgg cttctcttgc aactccggca ggcagtggca gcagccatga ccagttaggg 33300

ttcaatattt ggatcaatat gaaaggggct aggggaaagg aaagaaggag gttctctcta 33360

gggttagagg tttggctgtg tacggcggca acaagtgggt tgcaaagcag cgggggcacc 33420

acaagggcca caaggatgaa ggctagtggt gggagcctgg gaaatcgtgg agttaggaga 33480

tgaggagcgg cagtgatggc gtgatgcatc caatagcgtg ggaatcaccg gggatagaag 33540

catggtttgg aatttcggta attacgaccc ccaccaacaa gcactaatct cccccaaaat 33600

ttcaattttt tatcaaatat ttgtaaattt ggttaaattt tgttcaaatt cagtcaaaat 33660

attttataaa ttcagataat tttgattaga aaaatctgga aatcactaaa ttttcaaatt 33720

ttcgataacc actgaaattt tggaaatttc ggcggcaacc gaaattgcaa accctggata 33780

aaaggatgct atggtaaggg aggttcacat ccactattgt aactagttga aaccccacgc 33840

gttgctgcgg gattttgtat caatactttg gagaaatgaa aataccatat taaaagaaag 33900

agaatgatat gatggttgat acttacaaat attgagcaaa tacttgtaag taaaacttca 33960

gccgagccaa tatagtcgaa aaataaaata gggcatgcaa tattaggaac tgaattgcaa 34020

cgtcttcgtt attctccaaa agaaatagtc atctagttat ttaatctaaa aaaatgttgc 34080

atatttactt ggttaagtgt tgtgcttgtt ctgaactcct ttttaagtac cgtacataac 34140

ataattttgc taacaactgc agtgattact tgattagtga aggagcattg acggcttcat 34200

atataaaagt atgaacagat agttccagac catagaggtg acgaatactg tgtaattatg 34260

agttcagagg atggtgtaag gtatttgtgg tctgttatga atgctgaagg gatgttaaga 34320

ggctcatgaa gggatgctaa gagactcgtt aaatctcctt gtggcctaat taattgtgtt 34380

agttttccat gtaatttctc aagcggaatc gaaaaggaga ggaaatggat aggggcaagg 34440

tgatgagatg gcaatctgtg ccaccaataa tcaataatca atcctatggt gagaagagaa 34500

atcaatgaca tggcttcttc taaacacagc taatggtctt aactttctag aaagaaggaa 34560

taacattacc attacagaaa ctgattacca ttacagaaac tgatgtgtaa gataatagga 34620

cggcgggacc cacgcaccaa tgatcataac ctcaaccatc gtgaggccag gcacctctct 34680

gctgcaagtc ttgctagtgc tagcgaagta ggagtagacg acagtcctaa aaattgatct 34740

ggcccatcca aaaagttatt cagtggagag gagtctttca gtcaaatatt actccgtcac 34800

gttgtctagg agtagtacct agacaacggt ccgaaataga ttaatgttcg aactgatgat 34860

aagcaaaggt aaactacgat acgattttat acaaatgatg ataaattaat catatcacgt 34920

acaaatatcg tacacactga cccactgatg catgcgttca ggtggcaaaa agcgatcaat 34980

atatcagacg tggcacacac gtgcaccaca cgtgtgatac tatttccttc acttgcattg 35040

gacccactta ttcgtagctt acgttcgctc gctcgctcgc cgacggcgac gctcatgcgg 35100

cggcgccggc gtagagatgc gccggcagcc tgggctcggc gacggcgacg agcggcacct 35160

tccggcgcgt ggacagcccg accagctcct ccatgctcac gtcctccgcc gccacgccgt 35220

ccggcagccg ccacgcgaac ccctgcacca agctcgccac gcccagcgcc accatcttca 35280

tcgccaggtt cgtcgccggg cagacccgcc gccccgaccc gaacggcagc agctcgtagt 35340

gctgcccgcg cacgtccact ccggcggcgg cggcgccgcc gccggggagg aagcgctccg 35400

gcaggaacgc gtcggggcgg tcgggccacg acgccgggtc gcgcgcgatg gcccacgcgt 35460

tcaccagcac gcgcgcgccg gcggggacga cgtagccgcc ggcgatcacc gtgtcctcca 35520

tggcgtggtg cgggactagc agcgggccca ccgggtgcag ccgcagcgcc tccttcacga 35580

cggcgtcgac gtaggggagg tcggggatgt cgcgctccgt cacccaccgc gccgtcccga 35640

ccacgcggtc gagctcgtcg gtggcggcgg ccatggcgtc ggggcggcgg aggagctccg 35700

ccatcgccca ctccatcgtc accgcgctgc tctccgtgcc gccggcgatg atgtccagga 35760

tgaacgcctt cacgccgtcg cgcgtcagcc tcgcctccga gtccgcgccc gcgcccgcgc 35820

tgccctcctc ctcctcgccg gagagttgca gaagtacgtc gacgaggtcg cacgccgccg 35880

ccgcgggctg gccgccgtca ccggcggccg ccatgctcgc ctcgtgctcg tgaaggatct 35940

gctcgaggaa ccggtcgtac atcttgctca gccgcttcat cctccggcga aacccctgca 36000

cgtcgagcca ccccagcaag gggacccact cgccgacgtt gctcaccgcg ccggacacct 36060

cgaacgcctc gtcgagcgtg cgccggaacg cctcgccgtc ggcgctgccg tagacgcccg 36120

accacttgtc gccgaccgac atgcggagga tgttccgcag cgtcgcgctc gcgaggcgct 36180

cccgcacggc gacggcgcgg ccggcgcggc cgaacaggtc gcgggcgagc gccccgatct 36240

cctccgcgcg gatgcgctcg taggagtcga cgcggcgcgg ggagaagagc tcggtggcgc 36300

agagcttgcg cgccatgcgc cagtaggcgc cgtacggggt gtgcacgatc cccttgtagt 36360

tgtacgcgac gagctcgccg gcggcggtgc gcgcgcggtc ggcgaaggcg gcgtcgtggg 36420

tcttgaggac gagcctggcg gcgccggcgg aggaggcgac cacggcgtcg aacgagccca 36480

gccggaggtg catgagcggg ccgtggcgcg ccgcgagcgc cgccagggcg cggtgcggcg 36540

ggagcgcgcc gccaaggcgc cgaggctgcc gaacaccggc cacccgcgcg gcccggcggg 36600

aggttcagcc gcgccttcgt ccggcgccgg cggccacgca acgaaaccac ggcgacgccg 36660

atgaggaggg cggcgatggt ggcgacccaa gaagacatgg ccatgctcaa cgagagcgct 36720

ggtgcgagct ccattgtttg tggaaagttc ttgtgattgg agtgtgcacg agttgcctag 36780

agctttcatg gtgccaggct cttatatagg cgtatgagtt gatggcttga tgcagctaca 36840

attcagcatt cgtcagattt aatttcactc ctaaactttg tgtccatctt actccctccg 36900

tttcatacta taagtcattt ttagtcaaaa ttttttaggt tttactaagt ttatagaaaa 36960

attagcaata tttacaacac taaattagtg tcattaaatt taacaacgaa catatttgat 37020

aatacgtata ttttgtatta aaattttaag tatatttttt tacaaattta gttatacata 37080

aaaaaaaagt ttgataaaaa aaatgtcaaa taacttgtga tatgaaagag ggagcaattt 37140

cctagccgcc accccaatcc cgctggcgtt gactccacac taatttcggc ttaacatgta 37200

catatctcta actgaaatag ttagaggtga tgaatcacat ttatgttgta atagtcatcc 37260

aatttgccgg acaatgaccc aatcaagttt aagttcaatg gtagtattac tttatgaaat 37320

tgacattaaa ccaccttatg aaagcatcta tttgtttgtg aagtggctga cattaagggg 37380

gtaaatccat ggggtaaagt tttggtgtgt cacgtcgaat atacggatac acatttgaag 37440

tattaaacgt agtctaataa caaaacaact tacagaatcc gcctgtaaac tgcgagacga 37500

atttattaag cctaattaat ttatcattag caaatgttta ctatagcacc acattctcaa 37560

atcatagagc attagctttg aaagattcgt ctcgcaattt acacgcaatt tgtataatta 37620

gttatttttt ctttgtattt aatactttat acatgtgtcc aaacatttaa tgtgatatga 37680

tgaaaaattt taccagagat ctaagcatgc cctaaacata ctagagttag gaatagaaca 37740

tatctagctt aatccaaaaa aaaaaaagaa aacagggact accctcgaaa gctatagaat 37800

taaagctgat tagcaaattg ggggagatct acttgactac ttttcttcga atcacaatga 37860

ggccccgttt agttccccaa aatttttttc ccaaaaacat cacatcgaat ctttgcacac 37920

atgcataaag cattaaatat agataaacga aaaaactaat tgcacagtta ggggggaaat 37980

cgcgagacga atcttttgag cctaattagt ccatgattag ccataagtgt tacaataacc 38040

cacatgtgct aataacgaat taattaggct caaaagattc gtctcgccgt ttccaagcaa 38100

gttatgaaat tatttttttc attcgtgtcc gaaaaaccct tccgacattc ggtcaagcat 38160

ctgatgtgac acctaaaaaa ttttcttttg gcgaactaaa caggccctga atgcggattg 38220

ctcacacgca tgattgctca aaaagtcaaa cgccacggct aggcaattgg actagcctcg 38280

gaaagttacg aagggtttgt tcagcagtta cacgtaaaat agagcggtgg attagtacat 38340

atttaaatac taatttaaaa tttttaaaat ggattaatat attttagtac atgatcaatt 38400

aagtattagc ttaaacactt taaaatggat taatatgttg tagtatatga ttaattaagt 38460

atcatcttaa aaactttaga aatggttttt taaacaactt ttctatagaa ttattttttt 38520

ttaaaaaaaa gtaaagatac accgtttaga aattcaggaa gtgtgcgcgt gaaaaacaag 38580

gctgtaagtt tgaaactctc attgctgaac acactcgaag aacacgtggg cactccgccg 38640

accggttgta gtcacgaaac accgcaaggc atatctcaaa cggggggcct tgccaactag 38700

cagggaatta atatagcaat tgtagggtgc acgattttgg tcaaagctgt gcttccttca 38760

caaccaatat acctcctaat ctaacaacac tcaaggtcag cgaggaatcc ttggaggtgc 38820

ttgataaaca aaggaagtgt tttttttagg acgggtataa acaaaggaag tgtttttttt 38880

aggacgggtc agaaaaactc acgagaggaa agtggaaggt gtcatagtta tggataagat 38940

ataccctcta ttcttggatg tacgatggat accgatacag tatacccgct cgtatacgga 39000

tagtttccgt atacgttaag gaaatctatc acgttataga aacagaattt acggatggaa 39060

ggagtcctac tcggacgaga ctgggtcttt accatatcgt gaggggtccg atgtctccga 39120

gtcctacttg gagatcaact gacccacggt atataaggga cccctgggca ggacctaaga 39180

catcgaatat tactgccaac acacccccca tagcctacgg agccagagca cgcaagagcc 39240

aagtcgccgg gagatctagt cgaaccaact tgactacgct ttctgttaaa tatgtgatcc 39300

gaattttggg cccacaatat attcggatta gtttcctagt aggagtccta ttaggtgtct 39360

ttctattatc ccctatatat actcttgtaa gccgtacggg aaggggtgac gttccattgt 39420

gattccccta cgtttgtaat catctcctct atagtgatca ttgccggtcg gcacccgtgg 39480

tttttcccgc aagggttttc cacgtaaaat tcgtgtctcc gttgtgcatc tattttcata 39540

acaagtggta tcagagcatt ggagattcga tcaacagcta tcccaccgtc gttcttcaat 39600

tcggcgagat tatccaccag tgcatgttca tcatcggcga cggcaggcgc gtgtcgtcgc 39660

tcgtctcgtc gattcgtctc gtcttcgtcg tgctgcctac gcacgcgaga aaaaacagga 39720

ggagcagcag ccggtctcca caccacacgt cgggggagga gaaaaagcag cagaaaccaa 39780

tctaattgcg cacggatccc aaagtgaaga aaaagtaggg caagtcagtc agagccatca 39840

tcgccctgtt ctgtgaattc aatttcacgt tgagaattgc tacggccacc taagcttgtg 39900

taccaggaga ttccctgatc tacgctacgt gcacacagaa attgcaccca gaggttcgcg 39960

attttgctag gtttacccta atttcttact agcaaatttc aggattaatt cctatggcga 40020

gtttgaagta tgatctaccg ctcctggacc gagacacaag attctcactt tggcaagtga 40080

agatgcgagc cgttcttgca cagcaagacc tcgatgatgc gcttctggat ttgacaagag 40140

gactcaagat tggtccaacg atgagaagaa aagggatcgt aaggctatgt catatattca 40200

tcttcatcta tctaacaata ttttgcagga ggtgcttaag gaggagacag ccgctgggct 40260

gtggttgaag ctggaacaga tatgtatgac taaggatctg accagtaaga tgcacctgaa 40320

gcaaaagtta tttttgcata agttgcaaga tgatgggtct gtaatggacc atctctctac 40380

tttcaaggaa attgttgctg accttgagtc tatggaggta aagtatgatg aagaggattt 40440

aggccttatt ctattgtgtt cattgcctag ctcatatgca aacttcaggg atactatact 40500

ttatagtcgt gatactctga ctttaaaaga agtttatgat gctttgcatg ccaaagaaaa 40560

gatgaagaag atggtaccct ctgaaggttc aaattcacaa gcagaaggct tggttgttcg 40620

gggcagccaa caggagaaga acacaaagaa ccaatcaaga gataaaagct caagtagcta 40680

ccatgggaga tcaaagtcca gaggcaggta taagtcatgc aaatactgca agagagatgg 40740

acatgatatc tctgagtgtt ggaagctaca ggacaaagac aagagaactg gaaagtatat 40800

accaaaaggt aagaaagaag aagaaggtaa agccgcagtt gttactgatg agaagtctga 40860

tgcagaattg cttgttgctt atgctggttg tgcacaaact agtgaccagt agattcttga 40920

tactgcatgc acctatcaca tgtgccctaa tagggattgg tttgccacct atgaagctgt 40980

gcaaggtggt actgttttga taggtgatga tacaccatgc gaggttgcag gtattggaac 41040

agttcagatc aagatgtttg atggctgtat cagaacattg tcagatgtgc ggcatattcc 41100

aaatttgaag agaagtctca tctctttatg tactcttgat cgcaaagggt acaaatattc 41160

cggtggagac ggaattttga aggtaactaa aggctctctt gttatgatga aagctgatat 41220

taaatctgcc aaccagtacc atctgcgagg tacaactata ttaggtaatg ttgcagcagt 41280

ttctgattca ttatctaatt ccgatgctac taatctttgg catatgcatc ttgggcatat 41340

gagtgaaatt ggtttggcag aattgagcaa gcgaggattg ctagatggac aaagcatcgg 41400

gaagctcaaa ttttgtgagc attgcatctt tggcaagcat aagagggtga agttcaacac 41460

atctacacat actactgaag gtattcttga ttatgtgcat tctgatttat ggggcctgct 41520

cgtaagacat cttttggagg tgctcgttac atgatgacta tcgttgatga ttattcgcga 41580

aaagtttggc cttatttctt gaagcacaaa tatcaagctt ttgatgtgtt taaagagtgg 41640

aaaactatgg ttggaagaca aactgaaagg aaggtgaaac tccttcgtac tgacaatggg 41700

atggagttat gttctaagat atttaaatca tattgcaagt ctaaaggcat tgtccgtcac 41760

tacaccgttc ctcacacacc tcaacaaaac ggcgtagctg agcgtatgaa cagaacaatt 41820

atatcaaagg cccgttgtat gttgtctaat gcaggtttgc ctaaacagtt ttgggcggaa 41880

gccgtttcca ctgcttgtta tcttataaat cgatcaccta gctatgccat tgataagaag 41940

actccaattg aggtatggtc tggttctcca gcaaattatt cagacttaag agtatttggt 42000

tgtactgctt atgctcatgt tgataatggt aaattggagc ctagagctat taagtgcatt 42060

tttcttggtt atccgtctgg tgtgaaaggc tataagttat ggtgtcctga aactaaaaag 42120

gttgtgatta gtagaaacgt tgtcttccat gaatcagtta tgttacatga taaaccttct 42180

actaatgttc ctgttgagag tcaggagaaa gcaagtgtgc aggtggagca cttgatcagt 42240

tcagggcatg caccagaaaa agaagatgtt gctatcaacc aagatgaact tgttattgaa 42300

gattctaatt cttctactgt tcagcagtct ccaaaacgtt ctattgcaaa agataggccc 42360

aaaaaaaata ttaaacctcc tcaaagatat attgaagaag cgaacatagt tgcttatgct 42420

ttgagtatgg cagaagaaat tgaaggtaat gcagaacctt ctacatattc tgaggctatt 42480

gtttctgatg attgcaatag atggataact gctatgcatg atgagatgga gtcacttgaa 42540

aagaatcata cttgggaatt ggtgaagttg ccaaaggaga agaaacctat ccgttgcaag 42600

tggatcttca aaagaaagga aggtatgtct ccaagtgatg aggcaagata gaaagcaagg 42660

ctagttgcta aaggttatag ccagattcca ggtattgatt tcaatgatgt cttttcccct 42720

attgtgaagc atagttcaat tcgcacttta ctcagtattg ttgcaatgca tgattatgaa 42780

ctagagcaaa tggatgttaa gactgcattt ttacatgggg agttagaaga ggacatttat 42840

atggagcaac ctgaaggttt tgttgttcct ggaaaagaga acctagtctg taggttgaag 42900

aaatctcttt atgggttgaa gcagtcacct agacagtggt ataagagatt tgactctttc 42960

atgctttctc agaagtttag aagatccaat tatgatagct gtgtttatct cagagttgtt 43020

gatggttcag ctatatattt gcttctttat gttgatgata tgttgattgc tgcaaaagac 43080

aaatcagaga tagcaaagtt gaaggcacaa ttgagtagtg aatttgagat gaaggatttg 43140

ggtgcagcga agaaaattct cagtatggaa attactagag aaagaaagtt atatcttagt 43200

cagaaaggtt atattgaaaa agttattcgt cgtttcaata tgcatgatgc aaaaccagta 43260

agtactcctt tagctgcaca tttcagatta tcttcagatt tatgtccaca gtcagattat 43320

gatattgagt acatgtctag agttccatat tcaagtgcag ttggttcact tatgtatgcc 43380

atggtatgtt ctcgtcctga cttatcacat gcattgagtg ttgtcagtag atacatggct 43440

aatcctggca aagagcattg gaaagctgtt caatggattt tcagatacct acgtggtaca 43500

tctagtgctt gtttgcagtt tgggagatct agagatggac ttgttggtta tgtggattca 43560

gattttgctg gagatttgga taggcgaaga tcgctcacag gttatgtttt cactattgga 43620

ggatgtgctg ttagttggaa ggcaagttta caagcaacgg ttgccttatc tactactgaa 43680

gcagagtata tggctatttc tgaagcttgt aaagaagcta tttagcttag aggtttgtac 43740

actgagcttt gtggagttac gtcttgcatt aatatatttt gtgatagtca aagtgcaata 43800

tgctttacaa aggattagat gttccatgag agaacaaagc acattgatgt gagatatcac 43860

tttattcgag gtgtgattgt tgaaggtgat gtcaaggtat gcaagataag tactcatgat 43920

aacctgctga tatgatgaca aagccagttc ctgctactaa gtttgagctt tgctcaagct 43980

tggttggtgt tacagtatag tcctagagac tatttggcgc gcggtgattt tacctacatg 44040

aggtattttt tggtgattaa tgtttttatg ctacaagagg aaattcgtct caaggtggag 44100

attgttaaat atgtgatcca aattttgggc ccacaatata ttcggattag tttcctagta 44160

ggagtcctat taggtgtctt tctattatcc cctatatata ctcttgtaag ccgtacggga 44220

aggggtgatg ttcccattgt gattccccta cgtttgtaat catctcctct atagtgatca 44280

ttgccggtcg gcgcccgtgg tttttcccgc aagagttttc cacgtaaaat tcgtgtctcc 44340

gttgtgcatc tattttcata acactttcgt cggtatcatc aagttctact attccctttg 44400

taatatgtgg tttccattat ataatcccaa atcaactgga ttaaggctat tactttctaa 44460

ggggcctgaa ccagtataat ccttgtcttc tgtttgcttg atgtcgtatt acgtagatcc 44520

ttatatcaac ggaccccagt accctctttg tccggtctac gggtatcccc cgtcgacaga 44580

aggttaattg gataagaaca tgttcgccaa accgcgtacg acggattagg gtattgaacc 44640

tgaaaaaatt tgcaagggta acagtgtgcg aggatctgga caaacaactc tttgcactag 44700

caaccacaat aacagtagga gatggggcaa caattcactt ctgaaactcg gcctggatgc 44760

gaggccggtg cctaggcaca tagcaccatg agtatacaca atatcatgaa agaaaagcag 44820

gaccttgaag caaaccatat aggatgataa gtggattcaa gacctcgacc tctcgcccaa 44880

cagaggcatg accattgagc ttctaaacta acctgccgcc ctatggagca ttgtccaaga 44940

aattcaactc acacccaatt tgacaaaaaa taagctggaa actcaaaagc catggggagt 45000

actaagctag gttcacctac caagcgtaat tctttggctc gatcaacacg acctatgaga 45060

tgatcatctg gaaaccacga gcctcgagaa agtgtaaaat ctttgtatgg cttgtagggc 45120

aaaacagagt tcagatgtcc tactagctag ccaaatacaa cctattgccc gctctatcgg 45180

cacaccatgg aaacatccac ctgctagtgg aatgcagata tatgaagaga gtgtggacca 45240

tgctgttaac ctggatgcca caacaacagc tatacccgac aacgtggagc acaatcacga 45300

cgattcacga gtagcgtata tatagtgcta gcaagtgcca atggcttaaa aagggtcgat 45360

acgaggaatt aggtaaggag atgaaccaaa gagcgttcca acacaaaagg ctattgggga 45420

catccatgct tgcaaaaaaa tcaagaaaga ggtcaagacc tgagcttcag cagcagcaaa 45480

atgtctcaaa gattattccc tagctttcat tttattaccc ttgtataacc tccaaccttt 45540

atttttacat ttttgtcttc actcctcttc ctatattcaa tagaattggt acatgcatgc 45600

<210> 2

<211> 2205

<212> DNA

<213> Oryza barthii L.

<400> 2

tttgcccgga gaagacgagc tcacattgcc cggtctgtgg ccggcggcga tgtggtgcag 60

cgctgactgc ccgacaagcc ggggttccgg tgcagcgaag tccacacgct gacggtgggt 120

tggctggagg tggatggcag gcgaaagcta agcattgccc ggtcatcggg ccgacaacgg 180

cgacgtctct ggacgccgtg ttctctctta ctccgcccgt cccataaaaa ccaatctagt 240

attggatgtg acacatccta atacaatgaa tctagacata acatccgatt tgtttttttt 300

ttttgggacg gagggagtag gtgtgttgtc aaagctttct tccctccctg ataggatccc 360

taggttaaaa tcttagtcta gatcccctag agatctagat ggacaacaac gatgctccct 420

acgtcgtgtt tctccctgat gaaggcgttg tctagaaggc ctctttgccg tttaatttgc 480

ttccaaatag tttggtgaaa agcccttaaa tgacggcggg tcgaaaggtc cgtgcaaggt 540

agagggattt ttcttctctc tcacctttca tcctagtaaa gtctaagagg ctagctaggt 600

actcctttat tgcttcatag tacctagaca aaggaaatct gtacatgcat gccgattcat 660

taaaaaatga aaataaggaa actaatcatg gtagtgagta catactacta tgaagctgta 720

atttttaaat tgtgcaagtc tagctagttg actaataaat agcaaaaaaa aaataggctt 780

ttatagatga gttagaaaat ctagaaaaaa aatccctaca taaatcctta atttttaaga 840

taatggaata gagcatcccg gcctttgctc aaaaagctac agccaattat tacaatctca 900

cactcaaaat aagtgaagtt caaagatact taaaagatga aaccaaccat caagaagaaa 960

caacctaaag actgccaacc caagataaag agtacacaat tattgaattt tgtttgtgaa 1020

cctccatcca aaattgacaa aaagttgcat aaccgttgtc tcaagagtat gacatgcagc 1080

ctttaaaagc tcactatgtt cctcacactt ttgtagttat gtccaagacc ggagccaatg 1140

tgttgcactg aaaactacct gcatatatga aatagatggt gacttgtcaa aaatgacatc 1200

attcctactc aaccatagag cccaacatat agcagatgct cctatatgaa caagttttct 1260

tctttttttg tccaccccca gaagccaatt atcaaaaata tgagatatac catgaggaag 1320

gtaaagacta aaagaaaact gaactgctct ccataaaaac ttagcataat aacactcaac 1380

aaatagatgt tgaatagttt catttttcat acagaaacaa catctcaagc taccattcca 1440

attcctcctt cccaaattat ctttagttaa tacaacccct ttaagcaagt accacatgaa 1500

gattttgatc ttaagagaca tcctaagttt ccaaataatc ttatttctct caatataatt 1560

attgtttatt aaggctaaat acattgttcg aacagtaaat tgaccatttt gatgataatt 1620

ccatcttaaa caatcattca gttgactcaa ctgaatatgc acaatagaag cacacaagtt 1680

atgcctacac catgcctgcc gacataggtg tcttcttccc ccacaagcat atccatgcgt 1740

ggaatataga gtaagacatt tgttccataa gttaaacatg tattttatga ttgagtgatc 1800

aattagccgt ttcattcctc aatttaaaga gtataccctt agtctcaagt ttatatggat 1860

tacaccttaa gcacatacaa caatctcgtt tgaagttttg taataataat aagtagatcc 1920

aatccgcata gttatttttg gctattccac gcaatttgcg gtcattttaa aataaacgaa 1980

caattgtttc ttgacaaaaa aaaaataaaa aaggttcatt ttactcactc aactattcta 2040

ttggatcgct taatctccta aatatcctaa atagacaaaa ctttctggat ggccggttag 2100

agttagagtg gatcttcctt tcctcacaat tttgaacaat tccatgagca tatatatata 2160

tatgctagtg gcttaggcct tcacaggaat cagatcaatt tcccg 2205

<210> 3

<211> 25

<212> DNA

<213> Artificial sequence

<400> 3

caattgccat ttgcttaata ctctt 25

<210> 4

<211> 20

<212> DNA

<213> Artificial sequence

<400> 4

tgcatgcata cgatcactca 20

<210> 5

<211> 21

<212> DNA

<213> Artificial sequence

<400> 5

ggagaagacg agctcacatt g 21

<210> 6

<211> 20

<212> DNA

<213> Artificial sequence

<400> 6

aattgatctg attcctgtga 20

<210> 7

<211> 8917

<212> DNA

<213> Oryza barthii L.

<400> 7

atgtcggcac agatcgtgcc ggcgccggag catgtgtgct acgtgcactg caacttctgc 60

aacacaattc tcgcggtaat ttgtctgaat tgttgatccc tttctccttc tctctctctc 120

tctccccttc cttgatttcc ttgcctcctc ttttctacat gaagtttctt ttcttttttg 180

ggggggaact ctcgatgaag gtgcagatct acaagggcaa gggagatcga tgcatatatg 240

agatgattgg ttgcttggct tttctttaga attgtgtatg atttgggcag ttcatttgcg 300

tgatttcttt aatttttaat ttgtgcgaaa agatgtgtgt catgctgcca agagattggg 360

tttcgatata tatgttactt tttttttacc caattaccct tcgttttctt tctttaattg 420

tcgacagagt tcagatagct tcacctcctt tccttttcga ctcattaaaa ctctggtccg 480

gttttctctg aaagagagct tgtcgaccct aatttttttg atttagtcta tcatggagtc 540

atgggcatga ttacatatga atgggaaaaa gggcaaaggt catgatgatc ttgagaattg 600

agctgcttct ctctctttgc accattttgt gtcgtttgat tatatgggtt catgaattat 660

ttgggcgagt tgatgaactt caattaactg gtacgtacta gagtagctag ctctagatca 720

tccttgtttt ctctctcgac tcttaatttc cttcagaacc tagcgtagct gatgctagat 780

cgatcttacg tacatatgaa gtgcagttga ttttcttgcc ggtcgactgg tactttatat 840

ggctaccata tataagagta tcgtcgtttc aggtgttatt actagtaatc gaattgctac 900

aaattaaaga gcatgcaata ataacacata actttaatta gtatatgcaa ctactgtgtg 960

taatgttctc ctgcttgcct cttagatcga tcatctttta aaatccactt gctcatcccc 1020

tctaagcaca aatacagcat ctgaaggact cagaaacggc aacctctcga agtacggtac 1080

tgtgttacaa gaattaaggt gcaaattgca tcaataattc ctgtatgtat gtgttcccgt 1140

gctagctgtt ccactcattc gttcttcccg tgctatatct tggctttctt gatggtttct 1200

ctttcttttt tttcttttct ttttggtgca atgcatggtg tgagtttaag gactaagtac 1260

aaataactaa aactacataa ttaaaatgaa gcagaagcag ctagtaacca taaggcaaaa 1320

agattgttac gtccttccaa agaaaaaaaa ttgtggtttg agcttgagct agcttcttct 1380

ccttcctcct cttctttttt tccagagaga gagagagaga gagagagaga gagatgcttg 1440

agatggcatg gccggctagc ttcactcaat acatgcaagg gaattagatc ggtccttata 1500

tatagactat agtcatcagt gatgtgttga actggtctga atggcgtttt aactagctaa 1560

tccccatgtt gaactcatat atacactatc aggaattcgt ataaacattt taatttctct 1620

ctctattttt ttttctgttt tcccacttct tcttggatct tgccttgctt gctttggaag 1680

tacacgctgc tagactagtg atatgatatg atcgatcccc ctcagtcagt cagtctgatc 1740

tgcatatatg ggcggcgaga gtgctacgaa atatcgcaaa ttcgtgcaca cacacaacat 1800

aacaaatctc tagctagttt tctacggttc tcaatgaatt gaagtatata tatctttata 1860

tatagtgcat atcaattatt cttgttgatt aattagttag ttccgttcga ttctcgacat 1920

gttaacttct tggcacaatt acatgcatac agtctttata taggagtata tatatcttgt 1980

tgatctgcgc tacttgcatg cttcaatata tctttcagac tttcagaaga gaaattatat 2040

tccttatctc tgaagtctaa atagctactg tagttcgcaa gaactttatt ggaggccatc 2100

ttttactcca tctcaatctc tcctactccc tccagaaaga taatcaaaat aagcgcattt 2160

gggaacaaat atagctagtg accattaatg agagtcgggc ccaaacaatt caagtattgt 2220

aaataccata acagcaaaaa gtagaataca ccaaaatgag tctattacca gttccaggaa 2280

gagcacacca aatgacagaa actattactc aatcccaaaa caaattaatt tttactatag 2340

ctaattagct atgaacttgg aaagtagtga ccgggatata gagagtgtac acgccaacga 2400

caaatgcaga gccgtacgta ctgacttcct gcatctacat cacccactgt aatgtcaatt 2460

gctgaggcgt agtacacatg tctagtgtag atagtacatg tatagctaca tatgcatcca 2520

tgcatgatct ttcacaagat cttaccagca tgtctcaact ttactactgc aactccaata 2580

cttatatatg tatgtcttct tgctctctcc taccgaaaga tgaaaaaaag gggattgaat 2640

catcttgtgc attgcatgac ccacacttaa atgtactccc tccatccaaa aaaaactcaa 2700

ccacccctcc taagacaaca cccctcctaa gacaacgaat ctgaacaaaa ggtagtctaa 2760

attcattgtc cacatcctct tttaggttga gttttttttg gatggaggga gtacccacac 2820

aaacacacat gcaggagagg agatgttgtg cttgagagtg aattggcttc tgcatgaagg 2880

gtcactgtct ctagctagca cctttctttc ctgcactgtt catgcatgta gctgaaggct 2940

ttgtgatttc ctactactgc tactactact ctgtggagag gccagacaga gaggcatgac 3000

atgacatacc atgcaaatgc atggcttctt cctttaaaaa agtcaccaat agatatgttg 3060

tgaggttgtt tgcttctgtc acaaccaact actagcagct agcccttttg tgctagatat 3120

gtgtggagtg aagtgtgatt tgagctaact cccctcatgg gagcacacac aatcattctc 3180

attcagtcac tttgcttaat tatttctttc ttctttctta tgggagtgag tgcccaaaaa 3240

gtgagtgaat gtagggaatc aaacaaagta gaggtactgt gctcttcagg tttgggaaga 3300

aaaatgggaa cggcaaggga ccaatgtgtg caggcattgg aaaagggtgc cccaattctg 3360

atctgatcag atatatattc agatcagcag agcccctgat aaaagtcatg gaggaacagt 3420

tatccacatc taaagctata gctgtgtatt aatgtgtatc tgcagtctac accagcctta 3480

tgaaacatgc atgcatgcat gcatgccttt gagatttgat tttttacttc tcagattctt 3540

ttgtacaaat gcaaaaagaa aatgatcctt tggaatctag catttgttac atatttacaa 3600

tataaagtgc tatacttgtt tacttttcat tctacttgtt ccagcacatg catatgtaat 3660

tgtacacctg ctcaaattta caatgagtgg catatatatt atacatatta attacggctt 3720

acttgtattt cttccataaa agaacatgtc tagaatattt cttttcttga cagtaaatat 3780

tcaggacata ttatactgat tcttggaatc aatctacagg tcagtgttcc aagcaatagc 3840

atgctaaaca tcgtgaccgt ccgttgtggc cattgcacta gcctgttgtc agtgaacttg 3900

cgaggattgg tccaagcatt cccagcagaa gatcatttac aggttgacac atcaatatat 3960

actactcttt catgcatgaa attatggata cagatacaat gactacatta aatcatatta 4020

tctcttttta ataaaaaaac acacatatgt tgcctatata tatctagaat ccaaattaat 4080

ggcacttttc aattataacc attgctactg catgcttagt ctgatagagc tagaaatcaa 4140

cgaacaatta atgtatatgt accggagcat tcttcacata cccagctgga aaaaaatcac 4200

atgcagatcg atgtatgtta aactacccta tatatgtaga agtagtatag tactactagt 4260

atatgtagat aactactccc tccgtttcac aatgtaagtc attctagcat ttcccacatt 4320

tatactgata ttaataaatc tagacatata tatctatcta gattcattaa cattaatata 4380

aatgtgagaa atgctagaat gacttatatt gtgaaacgga ggaagtagat tttactcaca 4440

gagaaaaaca gaaaagacac catgcaatgc atgcatgggt catctcaagc ttgaatactt 4500

tacatgtaca gcagagacaa attaaagcat tgcactattt actcacagga aagaatttta 4560

aagctcttgc acatgccaaa atatatacta ggagtaccaa gccatatggc tagatcagct 4620

aggtagctca ggttgctcag ctgatcttat actacatgac ttgtgcccct ttgtcaaacc 4680

ctgcagggtt attccccgca aaaaaaaaaa tcctgtaggg ttattgataa atactagtag 4740

atgttttgca tttgttttta tatgctacta tatataatgg aaaaaatcct gccacacaag 4800

tacttgaatt aaatttccaa tatgaaaagc acgagtttcg aattccggca ataactacat 4860

gttaggtttg atatactccc tccgtcagta aggtgtgttt agttcacgct aaaattggaa 4920

gtttgattga aattgaaaag atgtgacgga aaagatggaa gtttgtgtgt gtaggaaatt 4980

tttgatgtga tggaaaagtt ggaagtttga aaaaaaagtt tggaactaaa ctcggcctaa 5040

atatgaggga tacgttactg tagaatgtac aaaattgatt cgccaaattc atgtttagag 5100

attttcatgt ttgcttcaca aaacaatcaa aagccatgca gatttttcga aatgatctga 5160

acagtaacca ccaatgtttt acttaatttt cttgtcttga cttggttata atttccatgt 5220

aaatacacac taagtagcat ctagaggaaa aatgtgtgac tttaagttga ttaacttgct 5280

tcttacattt tcctcaggac aatttgaaga tgcacaatat gagcttccgc gaaaactact 5340

ctgaatacgg ctcatcttcc agatatggcc gagtgccaat gatgttctca aaaaatgaca 5400

cagagcatat gctgcacgtg cgacgtaaga caatgcatat gaacctgcat aataaattta 5460

gtgtacatgt gcgacggagc catatttcta gagattattg attttactat atgaagcaat 5520

ataaaggtat acaaatgcta ggggaatatt gcatatgttc acatatacat gctgggtgca 5580

tataccactt ttcttctcta aacccttttt atctttgttg ttttgctcag acaactgggg 5640

tacgtgtgtt acttcttaat taacacaaat acatgtatag agaaattata ctggcttaat 5700

caattcctga gttatgcact tttttaaaca gatatattta gaaaattcga cgagggataa 5760

acctctacta aaaagtcaga aaaatagaaa atttaaacaa gttgcattga ttcagctaga 5820

ggtggataat aaactagcta ggctcacaag ctggcttgtt aagctcgagt tttatatcaa 5880

tttaacatgt attaatctat tatatgttca cataatagta aacactactc cctctgtttc 5940

atattaaagt cgtttcatat tataagttat tttgatttta taaaaaaaag tcgttttgat 6000

tgttttccta gctagtcaaa gtaatttaag tatgaccaag tttacagaaa aatataacat 6060

ttctaataca gaacaaacat attaaaacat attcaatgct agatttaata taactaattt 6120

ggtgttttaa gtgttgcaaa aggtttctat aaatttaatc aaaactttaa aaattttgac 6180

tagaaaaagt caaaatgact tattatgtga aacggaggaa gagtaactag taaagtacta 6240

actagacatg aatagtttga aagtaatttc aagaagtatt atccatagta taagtattga 6300

ctaataggca ctcggtatat atggaaaata tggtgtgtct agttaagcta acaagctaaa 6360

aatccatctc acaagttcga gtcgatcagc caagccacaa gctttgagtt tttttcccca 6420

accctagcta ggcttagtaa agcttgttcc acccaagatg cgcgtctgct gttattgatc 6480

acgttaagtt ctgttaaagt ttatggtccc catcgcttgg cttatttcct aataagccaa 6540

aacggcttat tagaaaataa aaatgaattt gtaggcaaaa cttttataat tgtgttcttg 6600

gtgacttaaa agccaatgtt gaaaagaaac tacgttgaaa atatctcaaa atcaatctca 6660

aaattaagct tgaaaattta aaatttggct ttttctttgg ttgattaggc catccgatgg 6720

gagcctatac tgggctgtat atagaaatta ttcttatatg aattaatgaa tgaaacgaga 6780

tgtgcatgtg tgcgtgagtt ctagctagta gctagtacca tgcatggttc ggttaaagat 6840

ttttccgtgc ggtcaaaata catttttcag tgcggaggtc ccacccgtct gtaccctgca 6900

tgtctgcgaa aatccatatt ttcgcgtgcg ggcggcctaa ccgcatgaga taatccatct 6960

tcccgtgcgg tcgggttaag tagaccacac gggataatcg attatcccat gcggtcaaat 7020

tataccgccc gcacgcaaaa aaaaactgaa aaaaaataaa atcaaaaaca ctaaccctaa 7080

ccctagaaat ctagaatcgc ccgctgccgc tgctcgtgtt cgccatcgtc ctcatgtcgt 7140

cgtcgtggtc gcgccgtcgc cgctctcatc atcgtcgttg tggtcgcgct gccgccgctc 7200

ccggccgccg tcactgctcc cggttgccag cgcggcgcgg atccgaccgg tcacccgccg 7260

cgccgtctcc gccagcagcc gtcgtgccgc ccgctcccgg ccgccgcctc gttggcggca 7320

agccgggcaa ctcccgcgcc cgtgcgctcg gtggggaggg gaggagctag aggaaaggca 7380

gagagagaga gaggagtgat tgagggtgaa taaaagggaa aaggaataga gaagatgaaa 7440

agtttaaagt gagtaagaga gaaaagaggg agaggtattt tcgcgtgcag tccacttaag 7500

aggcccgcac gcgaattcgc gtgcgggcct cttaagggtc agcacgcaaa aataggctta 7560

tttttgcgtg cggaccgtta agaggcttgc ctgcgaaaat cgatatttgc gtgcaggtct 7620

cttaagagtc cgcacgcgaa aatgggaggc caatttttgc agacgcgcgc gaaaacggtc 7680

cgtctgggaa aatggaagct ccttgtccag aaaaatgttt tgtgtactag tatatatttg 7740

aaaatactga tgcatgaact agctacttga gtactatttc attttgtgag cccaaaaata 7800

ttgacgtacg aactacttga ctactacgta ctcctattaa tttcttgaaa aacctaggag 7860

tagtatacat actctcaaga gtatgtgctt tagatagtaa ttaaacagta tctggatgct 7920

tatacttttt ttttacaaat ctttttcata caacttgcag ctccagagaa gaggcagcgg 7980

gttccttcag cttataacag atttatcaag tgagtttgat ctcgagacaa ttcgatcgat 8040

ccatatatat atatatagat gatctagaca gcttataaca tctgagagtt ttcagataat 8100

tgatataaat ctgccgtatt ctatactaag agagtgtttg attttgacac ccactaatat 8160

atcactacag ggaagagatc cggaggataa aagcaaacaa tcctgacata agccacagag 8220

aagccttcag tactgcagca aagaatgtga gtacatgtgt agctagggga aacaaattac 8280

tatcttaaac ttttacatat actgatataa tagctgttct tttttttact acaaactata 8340

atgcaaattc tcacacactt tggcctggtt tagttcccaa ctttttcttc aaacttctaa 8400

cttttccatc acatcgtccc aattttaacc aaacttctaa ttttagcgtg aactaaatat 8460

acccttgatc attgctgaat atatattgat agaagtatgt gagccatata catagagtta 8520

catatcaaca atactacata ttgttgactt tgtatgtcac tgctagcaag atattaaatc 8580

catggcaatt gccatttgct taatactctt atacatatag tagtgtccat attgatcact 8640

aaatactcag tctatatgaa tttaatttat ataacatatt cgtaaaaaaa atagtggtca 8700

aagttatata tttaagacca tgtcatagtc taaaccatca tctacttctg actagaggga 8760

gtaactctca tctcatatgc atcctacttt tttttttttt ttgcagtggg cgcatttccc 8820

gaacatccat ttcgggttag gctcccatga gagcagcaag aagctcgatg aggccattgg 8880

agctcctagt ccccaaaagg ttcaaaggct gtattga 8917

Claims (2)

1.一种鉴定或辅助鉴定非洲稻落粒性的方法，包括如下步骤：检测待测水稻的基因组中是否含有DNA片段甲，然后按照如下确定所述待测水稻的落粒性：含有所述DNA片段甲的水稻的落粒性为落粒；不含有所述DNA片段甲的水稻的落粒性为不落粒；

所述DNA片段甲为SEQ ID No.1的第161-45560位所示DNA片段；

所述“检测待测水稻的基因组中是否含有DNA片段甲”的方法包括如下步骤：用引物对1和引物对2分别对待测水稻的基因组DNA进行PCR扩增，得到各自引物对对应的PCR扩增产物；检测所述PCR扩增产物；

所述引物对1为由SEQ ID No.3所示的单链DNA和SEQ ID No.4所示的单链DNA组成的引物对；

所述引物对2为由SEQ ID No.5所示的单链DNA和SEQ ID No.6所示的单链DNA组成的引物对；

若所述引物对1的PCR扩增产物含有大小为503bp的片段，且所述引物对2的PCR扩增产物不含有大小为2193bp的片段，则所述待测水稻含有所述DNA片段甲；若所述引物对1的PCR扩增产物不含有大小为503bp的片段，且所述引物对2的PCR扩增产物含有大小为2193bp的片段，则所述待测水稻不含有所述DNA片段甲。

2.根据权利要求1所述的方法，其特征在于：所述大小为503bp的片段为SEQ ID No.1的第20975-21477位所示DNA片段；

所述大小为2193bp的片段为SEQ ID No.2的第8-2200位所示DNA片段。

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