CN108587855A - A kind of open microalgae culture system of universality - Google Patents

A kind of open microalgae culture system of universality Download PDF

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Publication number
CN108587855A
CN108587855A CN201810192891.3A CN201810192891A CN108587855A CN 108587855 A CN108587855 A CN 108587855A CN 201810192891 A CN201810192891 A CN 201810192891A CN 108587855 A CN108587855 A CN 108587855A
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China
Prior art keywords
light
culture
culture pond
collecting device
light source
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CN201810192891.3A
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Chinese (zh)
Inventor
朱严林
钱育恩
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Shandong University of Science and Technology
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Shandong University of Science and Technology
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Priority to CN201810192891.3A priority Critical patent/CN108587855A/en
Publication of CN108587855A publication Critical patent/CN108587855A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00Means for providing, directing, scattering or concentrating light
    • C12M31/10Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Cultivation Of Seaweed (AREA)

Abstract

The invention discloses a kind of open microalgae culture systems of universality, belong to microdisk electrode technical field.It includes culture pond and light collecting device, is used to place culture solution needed for microalgae growth in culture pond;Light collecting device is located above the side of culture pond, for providing light source into culture pond, between light collecting device and culture pond light source is transmitted by connecting light pipe, light pipe, which stretches into, is provided with spectrum groupware at the tail end of culture pond, spectrum groupware is for light source to be allocated, so that light source is evenly distributed in culture solution;Light intensity detector is provided in the middle part of light pipe, to be detected to the light source that light collecting device provides.Light filling is mended carbon and organically combined by the present invention, on the one hand alleviates the insufficient circumstances of carbon source, on the other hand solves the disadvantage that light intensity exponentially decays in water, and a kind of direction is provided for microalgae large-scale production.

Description

A kind of open microalgae culture system of universality
Technical field
The present invention relates to microdisk electrode technical fields, and in particular to a kind of microalgae culture system.
Background technology
At the beginning of microalgae is born in several hundred million ten thousand years preceding world, although type is more, quantity is plentiful, and individual is small, and distribution dissipates, with As for long-term ignored.However, microalgae has photosynthetic efficiency high, breeding is fast, with short production cycle, can efficient capture environment and purification The characteristics of Eutrophication materials in water body, adapt to a variety of different growing environments, increasingly obtain the concern of people with again Depending on.And with to microalgae further study show that, microalgae pharmaceutical developments, health care of food, animal feed, environment measuring, Environment purification, biotechnology and regenerative resource etc. have advantageous advantage, and industrialized production has become can not The gesture of blocking.
About microdisk electrode mode, mainly cultivated at present with closed photo bioreactor culture as representative using opening pond, If CN102382755A discloses a kind of micro algae culturing device and microalgae culture method, an open raceway pond has a liquid Outlet, the liquid outlet connect a liquid circulation pump, and the liquid-inlet of culture liquid storage tank is connected by the liquid circulation pump;The training Nutrient solution tank top has a liquid outlet conduit, and culture solution is allow to flow back to open raceway pond through this pipeline.It is store in culture solution Pot bottom is equipped with gas distributor, is passed through various containing CO2Gas benefit carbon is carried out to micro algae culturing liquid.By controlling open race Road pond culture solution and culture nutrient solution volume ratio and circulation rate in culture liquid storage tank, come adjustable micro-algal cell illumination runway Residence time in the culture liquid storage tank of pond and brightness, realize the light and shade forced circulation of frustule.
Above-mentioned open culture apparatus relative close type bioreactor has simple in structure, small, the operation cost of investment It is low, it is not necessarily to the advantages such as cooling system and no oxygen excessive concentration risk.But from existing research, carbon source is insufficient, light-use Rate is low and harmful organisms invasion is still to restrict an important factor for microalgae expands culture.
Invention content
In order to solve above-mentioned technological deficiency existing in the prior art, the present invention proposes a kind of open microalgae of universality Light filling is mended carbon and organically combined, on the one hand alleviated the insufficient circumstances of carbon source, on the other hand solve light intensity and exist by culture systems The disadvantage exponentially to decay in water provides a kind of direction for microalgae large-scale production.
Its technical solution includes:
A kind of open microalgae culture system of universality comprising culture pond and light collecting device are used in the culture pond Place culture solution needed for microalgae growth;
The light collecting device is located above the side of the culture pond, for providing light source into culture pond, in the light harvesting Light source is transmitted by connecting light pipe between device and the culture pond, the light pipe is stretched at the tail end of the culture pond and set It is equipped with spectrum groupware, the spectrum groupware is for the light source to be allocated, so that light source average mark in culture solution Cloth;
It is provided with light intensity detector in the middle part of the light pipe, is examined with the light source provided the light collecting device It surveys;
It is being additionally provided with light compensating apparatus and carbon compensator at the light collecting device, the light compensating apparatus passes through pipeline one It is connected to the bottom of the culture pond, for supplementing light source into the culture pond;
The carbon compensator is connected to the bottom of the culture pond by pipeline two, for supplementing carbon into the culture pond Source.
As a preferred solution of the present invention, the spectrum groupware includes main pipe rail, is provided on the main pipe rail point Luminous point connects several bypass lines in the other end of the optical splitting point.
As another preferred embodiment of the present invention, the light collecting device is a collecting lens, and the collecting lens is one adjustable Save the concave mirror in direction.
Preferably, the concave surface of the concave mirror is upward.
Advantageous effects caused by the present invention are:
(1) it is combined by both light collecting device and light compensating apparatus, nature is utilized and the reply of artificial composite light source system is more Become weather conditions, improves light and efficiency;
(2) pass through spectrum groupware so that light source is evenly distributed in culture pond.
(3) it by carbon compensator supplementary carbon source, and is passed through from culture pond bottom, utmostly improves microalgae luminous energy in water body Utilization rate, to realize the promotion of micro algae biomass and density.
Description of the drawings
The present invention will be further described below in conjunction with the accompanying drawings:
Fig. 1 is the structural schematic diagram of microalgae culture system of the present invention;
In figure, 1, culture pond, 2, light collecting device, 3, light intensity detector, 4, light compensating apparatus, 5, carbon compensator, 6, optical splitting point.
Specific implementation mode
The present invention proposes a kind of open microalgae culture system of universality, in order to make advantages of the present invention, technical solution Clearer, clear, with reference to specific embodiment, the present invention will be further described.
As shown in Figure 1, microalgae culture system of the present invention, including culture pond 1 and light collecting device 2, culture pond 1 are interior for placing Culture solution needed for microalgae growth, the culture pond are open, are preferably shaped to cuboid, and light collecting device 2 is selected in culture pond Side, lamp can be provided to greatest extent, light collecting device uses for reference device in the prior art, such as selects a concave surface Mirror, the preferably adjustable direction of the concave mirror, concave surface collects light source, will be supplied to culture pond after source-collector towards sunlight.
Light collecting device of the present invention is located above the side of culture pond, light source is transmitted by light pipe therebetween, by this It is arranged whether light intensity detector 3 is appropriate to detect light source, and light source detector 3 also can be according to required light source in culture pond on light pipe Light compensating apparatus 4 is adjusted;When light collecting device cannot be satisfied required light source in culture pond, light compensating apparatus 4, light filling dress are opened Set the lamp that preferably LED light etc. facilitates adjusting.
Also having carbon compensator 5 at the light collecting device, which is carbon containing pump tank, by with culture pond The supplementary carbon source thereto of pipeline two of bottom connection;
Be provided with spectrum groupware at the tail end that light pipe stretches into the culture pond, spectrum groupware be used for by the light source into Row distribution, so that the light source is evenly distributed in culture solution;Spectrum groupware includes main pipe rail, and optical splitting point is provided on main pipe rail 6, the other end of optical splitting point connects several bypass lines.
Preferably, above-mentioned light pipe multigroup fibre-optic bundle preferably by being made of by one as the present invention.
Above-mentioned light compensating apparatus such as LED light can detect light intensity according to light intensity detector 3 and be automatically replenished or decay to microalgae most Suitable extensive roots.
The substantially application method of culture systems of the present invention:
Natural light is transmitted to by light pipe in culture pond after light collecting device is collected, and during which passes through light intensity detector, detection knot Fruit can generate enhancing or influence of fading to follow-up LED light fillings, its intensity is made to remain at microalgae optimal light intensity.Into training The light source for supporting water body is assigned to via optical splitting point in every bypass line so that light source is evenly distributed in water body.Meanwhile it being placed in Pipeline two below light pipe supplements carbon dioxide in real time by air pump.
Carbon and light are passed through water bottom and utmostly improve the microalgae efficiency of light energy utilization in water body by the present invention, micro- to realize The promotion of algae biomass and density.The advantages that apparatus of the present invention are simple in structure with its, of low cost, and wide spectrum is applicable in a variety of microalgaes can For industrial production.
It uses for reference the prior art and can be realized in the above-mentioned part that do not address.
It should be noted that:Any equivalent way that those skilled in the art are made under the introduction of this specification, or Obvious variant should all be within the scope of the present invention.

Claims (4)

1. a kind of open microalgae culture system of universality comprising culture pond and light collecting device, it is characterised in that:The culture For placing culture solution needed for microalgae growth in pond;
The light collecting device is located above the side of the culture pond, for providing light source into culture pond, in the light collecting device Light source is transmitted by connecting light pipe between the culture pond, the light pipe is stretched at the tail end of the culture pond and is provided with Spectrum groupware, the spectrum groupware is for the light source to be allocated, so that the light source is evenly distributed in culture solution;
It is provided with light intensity detector in the middle part of the light pipe, is detected with the light source provided the light collecting device;
It is being additionally provided with light compensating apparatus and carbon compensator at the light collecting device, the light compensating apparatus is connected by pipeline one In the bottom of the culture pond, for supplementing light source into the culture pond;
The carbon compensator is connected to the bottom of the culture pond by pipeline two, for the supplementary carbon source into the culture pond.
2. the open microalgae culture system of a kind of universality according to claim 1, it is characterised in that:The spectrum groupware Including main pipe rail, it is provided with optical splitting point on the main pipe rail, several bypass lines are connected in the other end of the optical splitting point.
3. the open microalgae culture system of a kind of universality according to claim 1, it is characterised in that:The light collecting device For a collecting lens, the collecting lens is the concave mirror in an adjustable direction.
4. the open microalgae culture system of a kind of universality according to claim 3, it is characterised in that:The concave mirror Concave surface is upward.
CN201810192891.3A 2018-03-09 2018-03-09 A kind of open microalgae culture system of universality Withdrawn CN108587855A (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN201751415U (en) * 2010-02-05 2011-02-23 吴复元 Algae culture device
CN102382755A (en) * 2011-07-28 2012-03-21 中国科学院青岛生物能源与过程研究所 Microalgae culturing device and microalgae culturing method
CN102533530A (en) * 2012-02-17 2012-07-04 农业部规划设计研究院 Water curtain type exposure tower-shaped microalgae reactor
WO2013166638A1 (en) * 2012-05-07 2013-11-14 中国科学院过程工程研究所 Horizontal immersion cover type carbon supplement device and carbon supplement method used for microalgae culture in open pond
CN208055324U (en) * 2018-03-09 2018-11-06 山东科技大学 A kind of open microalgae culture system of universality

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN201751415U (en) * 2010-02-05 2011-02-23 吴复元 Algae culture device
CN102382755A (en) * 2011-07-28 2012-03-21 中国科学院青岛生物能源与过程研究所 Microalgae culturing device and microalgae culturing method
CN102533530A (en) * 2012-02-17 2012-07-04 农业部规划设计研究院 Water curtain type exposure tower-shaped microalgae reactor
WO2013166638A1 (en) * 2012-05-07 2013-11-14 中国科学院过程工程研究所 Horizontal immersion cover type carbon supplement device and carbon supplement method used for microalgae culture in open pond
CN208055324U (en) * 2018-03-09 2018-11-06 山东科技大学 A kind of open microalgae culture system of universality

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
方国涛 西安:西安电子科技大学出版社: "光纤接入技术", pages: 52 - 54 *

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Application publication date: 20180928