CN108587771A - A kind of Suaeda salsa seed oil extract technique - Google Patents
A kind of Suaeda salsa seed oil extract technique Download PDFInfo
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- CN108587771A CN108587771A CN201711237550.5A CN201711237550A CN108587771A CN 108587771 A CN108587771 A CN 108587771A CN 201711237550 A CN201711237550 A CN 201711237550A CN 108587771 A CN108587771 A CN 108587771A
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- demulsification
- suaeda salsa
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/04—Pretreatment of vegetable raw material
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/025—Pretreatment by enzymes or microorganisms, living or dead
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/02—Refining fats or fatty oils by chemical reaction
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
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Abstract
The present invention relates to vegetable fat extractive technique field more particularly to a kind of Suaeda salsa seed oil extract techniques, including ultrasonic pretreatment, the processing of microwave radiation technology enzymolysis, the preparation of composite demulsification bacterium full nutrient solution, demulsification processing.The preparation method of the present invention is handled by complex enzyme zymohydrolysis, and the oil yield of the fluffy seed of alkali is substantially increased after being handled using composite demulsification bacterium demulsification.
Description
Technical field
The present invention relates to vegetable fat extractive technique field more particularly to a kind of Suaeda salsa seed oil extract techniques.
Background technology
Alkali is fluffy also known as salt wormwood artemisia, seafood dish, is Chenopodiaceae annual herb salt tolerant euhalophyte, must containing human body in seed
Palpus aliphatic acid acid linoleic acid, oleic acid, leukotrienes and stearic acid, these nutritional ingredients are all the necessary objects for maintaining human body body function
Matter plays the role of body immunity before the decompression of human body hypoglycemic, expansion blood vessel, prevention and cure of cardiovascular disease and increasing etc. positive；Wherein
Linoleic acid plus linolenic acid is respectively the important metabolism production such as prostaglandin E PA (eicosapentaenoic acid), DHA (docosahexaenoic acid)
The premise of object has important role to maintaining health and adjusting physiological function.Therefore, Suaeda salsa seed oil is as nutrition
Wide development prospect is all had with oil or oil for health care, health value is high.
The method that tradition produces oil of plant mainly has water substitution, aqueous enzymatic method, mechanical pressing method and extraction method.But
All there are some disadvantages using the above method：Oil yield is low when producing vegetable fat for example, by using water substitution, the water content of grouts
It is relatively high, it is apt to deteriorate to result in waste of resources and pollute；Make grease from oil using the enzymolysis of enzymatic preparations using aqueous enzymatic method
It is released in material, to separating plant grease, enzyme preparation adds a more expensive；Using mechanical treatment to seed cytoclasis degree
Limited, oil yield is low, and protein can be made acutely to be denaturalized, and influences its further utility value；Although using extraction method
Oil yield is high, easily realizes large-scale industrial production, but have the residual of organic solvent in grease and grouts；Therefore there is an urgent need to
Develop the process that grease oil yield can be improved.
Invention content
The technical problem to be solved by the present invention is to：In view of the deficienciess of the prior art, providing a kind of Suaeda salsa seed
Oil extract technique can improve the oil yield of the fluffy seed of alkali using the technique.
In order to solve the above technical problems, the technical scheme is that：
A kind of Suaeda salsa seed oil extract technique, the extraction process include the following steps：
(1) ultrasound pretreatment：The Suaeda salsa seed cleaned, dried is pulverized and sieved, collect sieving after material and according to
Solid-liquid ratio 1:15-1:30 ratio is added deionized water, mixing, after supersound process, obtains solidliquid mixture；
(2) microwave radiation technology enzymolysis is handled：The solidliquid mixture of step (1) is taken, pH is adjusted, solidliquid mixture weight 0.1- is added
0.5% complex enzyme is digested, and is centrifuged and is collected free oil, emulsion, hydrolyzate and filter residue respectively；
(3) preparation of composite demulsification bacterium full nutrient solution：Composite demulsification bacterium is taken, is culture volume 2-8% according to inoculum concentration
Ratio be inoculated on culture medium, through everfermentation, obtain composite demulsification bacterium full nutrient solution；
(4) demulsification is handled：It takes the emulsion tune pH of step (2) to neutrality, demulsifying bacteria prepared by step (3) is added and cultivates entirely
Liquid, mixing carry out demulsification reaction, the trip that demulsification centrifuges the upper layer isolate of collection after reaction and step (2) is collected
It is mixed to get Suaeda salsa seed oil from oil.
As an improvement technical solution, step (1) be ultrasonically treated power be 200-400W, ultrasonic time 10-
30min, ultrasonic temperature are 45-80 DEG C.
As an improvement technical solution, the power of step (2) microwave treatment is 200-400W, time 5-15s.
As an improvement technical solution, complex enzyme in step (2) by pectase, cellulase, lignoenzyme and
Papain is according to 1-3:1-5:0.2-1:The ratio of 0.2-0.5 mixes.
As an improvement technical solution, pH when step (2) enzymolysis processing is 4.5-6.5, and temperature is 25-45 DEG C,
Enzymolysis time is 1-3h.
As an improvement technical solution, the composite demulsification bacterium of step (3) is by Rhodococcus sp PR-1, demulsifying bacteria LH-1 and withered
Careless bacillus is according to weight ratio 0.2-0.5:0.1-0.3:The ratio of 0.1-0.5 mixes.
As an improvement technical solution, step (3) ferment when fermentation condition：Shaking speed is 100-160r/
Min, cultivation temperature are 22-40 DEG C, incubation time 12-48h.
As an improvement technical solution, the demulsification temperature in step (4) is 30-45 DEG C, and the demulsification time is 0.5-
1.5h。
After using above-mentioned technical proposal, the beneficial effects of the invention are as follows：
Compared with prior art, the present invention mainly has the following advantages：
(1) cavitation effect generated the invention firstly uses ultrasonic wave pre-processes the fluffy seed of alkali, then again by micro-
Wave auxiliary aqueous enzymatic method carries out it enzymolysis processing, and pectase, cellulase and lignoenzyme can be to the cell wall constituents of cell
It is effectively degraded, grease and other active components is promoted to be released from cell；The breast separated and collected by enzymolysis processing
Shape liquid carries out demulsification processing by composite demulsification bacterium again, and what Rhodococcus sp PR-1, demulsifying bacteria LH-1 and Bacillus subtillis formed answers
Closing demulsifying bacteria, the lipopeptid class surface reactive material that generates carries out at demulsification in somatic cells itself or its metabolic process in fermentation
Reason, oil are precipitated out of emulsion, promote grease to release, substantially increase the oil yield of the fluffy seed of alkali.
(2) present invention process is simple, and demulsification efficiency is high, and the demulsification time is short, and oil yield is high, small toxicity, and pollution is few.
Specific implementation mode
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
Example 1
A kind of Suaeda salsa seed oil extract technique, includes the following steps：
(1) ultrasound pretreatment：The Suaeda salsa seed cleaned, dried is pulverized and sieved, collect sieving after material and according to
Solid-liquid ratio 1:15 ratio is added deionized water, mixing, (power 220W, ultrasonic time 12min, ultrasound after supersound process
Temperature is 45 DEG C), obtain solidliquid mixture；
(2) microwave radiation technology enzymolysis is handled：The solidliquid mixture of step (1) is taken to carry out microwave treatment (processing power 230W.
Time is 5s), pH to 4.5 is adjusted, the complex enzyme of solidliquid mixture weight 0.1% is added (by pectase, cellulase, lignin
Enzyme and papain are according to 1:1:0.2:0.2 ratio mixes) digested that (temperature is 26 DEG C, and enzymolysis time is
1h), it centrifuges and collects free oil, emulsion, hydrolyzate and filter residue respectively；
(3) preparation of composite demulsification bacterium full nutrient solution：Take composite demulsification bacterium (by Rhodococcus sp PR-1, demulsifying bacteria LH-1 and withered
Careless bacillus is according to weight ratio 0.2:0.1:0.1 ratio mixes), it is culture volume 2.5% according to inoculum concentration
Ratio is inoculated on culture medium, through everfermentation (shaking speed 120r/min, cultivation temperature be 23 DEG C, incubation time 12h),
Obtain composite demulsification bacterium full nutrient solution；
(4) demulsification is handled：It takes the emulsion tune pH of step (2) to neutrality, demulsifying bacteria prepared by step (3) is added and cultivates entirely
Liquid, mixing carry out demulsification reaction (demulsification temperature is 30 DEG C, and the demulsification time is 1.5h), and demulsification centrifuges receive after reaction
The free oil that the upper layer isolate and step (2) of collection are collected is mixed to get Suaeda salsa seed oil.What above-mentioned process conditions obtained goes out
Oil cut rate is 24.09%.
Example 2
A kind of Suaeda salsa seed oil extract technique, includes the following steps：
(1) ultrasound pretreatment：The Suaeda salsa seed cleaned, dried is pulverized and sieved, collect sieving after material and according to
Solid-liquid ratio 1:23 ratio is added deionized water, mixing, (power 285W, ultrasonic time 15min, ultrasound after supersound process
Temperature is 53 DEG C), obtain solidliquid mixture；
(2) microwave radiation technology enzymolysis is handled：Take step (1) solidliquid mixture carry out microwave treatment (microwave power 280w,
Time is 10s), pH to 5.8 is adjusted, the complex enzyme of solidliquid mixture weight 0.25% is added (by pectase, cellulase, wooden
Plain enzyme and papain are according to 1.5:2:0.5:0.2 ratio mixes) digested (temperature be 30 DEG C, enzymolysis time
For 1.5h), it centrifuges and collects free oil, emulsion, hydrolyzate and filter residue respectively；
(3) preparation of composite demulsification bacterium full nutrient solution：Take composite demulsification bacterium (by Rhodococcus sp PR-1, demulsifying bacteria LH-1 and withered
Careless bacillus is according to weight ratio 0.3:0.2:0.2 ratio mixes), it is culture volume 4.5% according to inoculum concentration
Ratio is inoculated on culture medium, through everfermentation (shaking speed 148r/min, cultivation temperature be 30 DEG C, incubation time 20h),
Obtain composite demulsification bacterium full nutrient solution；
(4) demulsification is handled：It takes the emulsion tune pH of step (2) to neutrality, demulsifying bacteria prepared by step (3) is added and cultivates entirely
Liquid, mixing carry out demulsification reaction (demulsification temperature is 35 DEG C, and the demulsification time is 1.2h), and demulsification centrifuges receive after reaction
The free oil that the upper layer isolate and step (2) of collection are collected is mixed to get Suaeda salsa seed oil.What above-mentioned process conditions obtained goes out
Oil cut rate is 24.17%.
Example 3
A kind of Suaeda salsa seed oil extract technique, includes the following steps：
(1) ultrasound pretreatment：The Suaeda salsa seed cleaned, dried is pulverized and sieved, collect sieving after material and according to
Solid-liquid ratio 1:25 ratio is added deionized water, mixing, (power 365W, ultrasonic time 25min, ultrasound after supersound process
Temperature is 70 DEG C), obtain solidliquid mixture；
(2) microwave radiation technology enzymolysis is handled：Take solidliquid mixture microwave treatment (microwave power 320w, the time of step (1)
For 15s), pH to 6.3 is adjusted, the complex enzyme of solidliquid mixture weight 0.45% is added (by pectase, cellulase, lignoenzyme
With papain according to 2.5:4:0.7:0.3 ratio mixes) digested that (temperature is 42 DEG C, and enzymolysis time is
2.5h), it centrifuges and collects free oil, emulsion, hydrolyzate and filter residue respectively；
(3) preparation of composite demulsification bacterium full nutrient solution：Take composite demulsification bacterium (by Rhodococcus sp PR-1, demulsifying bacteria LH-1 and withered
Careless bacillus is according to weight ratio 0.4:0.2:0.3 ratio mixes), it is culture volume 6.5% according to inoculum concentration
Ratio is inoculated on culture medium, and through everfermentation, (shaking speed 152r/min, cultivation temperature are 34.5 DEG C, and incubation time is
40h), composite demulsification bacterium full nutrient solution is obtained；
(4) demulsification is handled：It takes the emulsion tune pH of step (2) to neutrality, demulsifying bacteria prepared by step (3) is added and cultivates entirely
Liquid, mixing carry out demulsification reaction (demulsification temperature is 37.5 DEG C, and the demulsification time is 0.8h), and demulsification centrifuges after reaction
The free oil that the upper layer isolate and step (2) of collection are collected is mixed to get Suaeda salsa seed oil.What above-mentioned process conditions obtained
Oil yield is 24.56%.
Example 4
A kind of Suaeda salsa seed oil extract technique, includes the following steps：
(1) ultrasound pretreatment：The Suaeda salsa seed cleaned, dried is pulverized and sieved, collect sieving after material and according to
Solid-liquid ratio 1:30 ratio is added deionized water, mixing, (power 385W, ultrasonic time 30min, ultrasound after supersound process
Temperature is 80 DEG C), obtain solidliquid mixture；
(2) microwave radiation technology enzymolysis is handled：Take solidliquid mixture microwave treatment (microwave power 275w, the time of step (1)
For 18s), adjust pH to 6.5, be added solidliquid mixture weight 0.5% complex enzyme (by pectase, cellulase, lignoenzyme and
Papain is according to 3:5:1:0.5 ratio mixes) digested (temperature is 45 DEG C, enzymolysis time 3h), centrifugation
Free oil, emulsion, hydrolyzate and filter residue are collected in separation respectively；
(3) preparation of composite demulsification bacterium full nutrient solution：Take composite demulsification bacterium (by Rhodococcus sp PR-1, demulsifying bacteria LH-1 and withered
Careless bacillus is according to weight ratio 0.5:0.3:0.5 ratio mixes), according to the ratio that inoculum concentration is culture volume 8%
Example is inoculated on culture medium, through everfermentation (shaking speed 160r/min, cultivation temperature are 40 DEG C, incubation time 48h), is obtained
To composite demulsification bacterium full nutrient solution；
(4) demulsification is handled：It takes the emulsion tune pH of step (2) to neutrality, demulsifying bacteria prepared by step (3) is added and cultivates entirely
Liquid, mixing carry out demulsification reaction (demulsification temperature is 45 DEG C, and the demulsification time is 1.2h), and demulsification centrifuges receive after reaction
The free oil that the upper layer isolate and step (2) of collection are collected is mixed to get Suaeda salsa seed oil.What above-mentioned process conditions obtained goes out
Oil cut rate is 24.26%.
In order to preferably prove that using extracting method of the invention Betacyanins product can be improved when extracting anthocyanidin
Purity and recovery rate, the present invention have done 4 comparative examples simultaneously.
Comparative example 1
As different from Example 3 without carrying out demulsification processing using composite demulsification bacterium, remaining condition all same, gained
Oil yield is 22.30%.
Comparative example 2
As different from Example 3, demulsification processing is carried out only with withered grass formula bacillus, remaining condition is identical, gained
Oil yield be 23.93%, be demulsified time 2.5h.
Comparative example 3
As different from Example 3, demulsification processing is carried out only with demulsifying bacteria XH-1, remaining condition is identical, and gained goes out
Oil cut rate is 23.94%, and be demulsified time 1.8h.
Comparative example 4
As different from Example 3, demulsification processing is carried out only with demulsifying bacteria LH-1, remaining condition is identical, and gained goes out
Oil cut rate is 23.91%, and be demulsified time 2h.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement etc., should all be included in the protection scope of the present invention made by within refreshing and principle.
Claims (8)
1. a kind of Suaeda salsa seed oil extract technique, it is characterised in that the extraction process includes the following steps：
(1) ultrasound pretreatment：The Suaeda salsa seed cleaned, dried is pulverized and sieved, collects the material after sieving and according to feed liquid
Than 1:15-1:30 ratio is added deionized water, mixing, after supersound process, obtains solidliquid mixture；
(2) microwave radiation technology enzymolysis is handled：The solidliquid mixture microwave treatment of step (1) is taken, pH is adjusted, solidliquid mixture weight is added
The complex enzyme of 0.1-0.5% is digested, and is centrifuged and is collected free oil, emulsion, hydrolyzate and filter residue respectively；
(3) preparation of composite demulsification bacterium full nutrient solution：Composite demulsification bacterium is taken, according to the ratio that inoculum concentration is culture volume 2-8%
Example is inoculated on culture medium, through everfermentation, obtains composite demulsification bacterium full nutrient solution；
(4) demulsification is handled：It takes the emulsion tune pH of step (2) to neutrality, demulsifying bacteria full nutrient solution prepared by step (3) is added,
Mixing, carries out demulsification reaction, and demulsification is centrifugally separating to obtain Suaeda salsa seed oil after reaction.
2. a kind of Suaeda salsa seed oil extract technique according to claim 1, it is characterised in that：Step (1) is ultrasonically treated
Power be 200-400W, ultrasonic time 10-30min, ultrasonic temperature be 45-80 DEG C.
3. a kind of Suaeda salsa seed oil extract technique according to claim 1, it is characterised in that：Step (2) microwave treatment
Power 200-400W, time 5-15s.
4. a kind of Suaeda salsa seed oil extract technique according to claim 1, it is characterised in that：It is compound in step (2)
Enzyme is by pectase, cellulase, lignoenzyme and papain according to 1-3:1-5:0.2-1:The ratio of 0.2-0.5 mix and
At.
5. a kind of Suaeda salsa seed oil extract technique according to claim 1, it is characterised in that：Step (2) enzymolysis processing
When pH be 4.5-6.5, temperature be 25-45 DEG C, enzymolysis time 1-3h.
6. a kind of Suaeda salsa seed oil extract technique according to claim 1, it is characterised in that：The compound of step (3) is broken
Newborn bacterium is by Rhodococcus sp PR-1, demulsifying bacteria LH-1 and Bacillus subtillis according to weight ratio 0.2-0.5:0.1-0.3:0.1-0.5's
Ratio mixes.
7. a kind of Suaeda salsa seed oil extract technique according to claim 1, it is characterised in that：When step (3) is fermented
Fermentation condition：Shaking speed is 100-160r/min, and cultivation temperature is 22-40 DEG C, incubation time 12-48h.
8. a kind of Suaeda salsa seed oil extract technique according to claim 1, it is characterised in that：Demulsification in step (4)
Temperature is 30-45 DEG C, and the demulsification time is 0.5-1.5h.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102286536A (en) * | 2011-07-14 | 2011-12-21 | 同济大学 | Method for extracting cell wall combined biological demulsifiers |
| CN102796613A (en) * | 2012-08-14 | 2012-11-28 | 东北农业大学 | Demulsification method by soybean grease microbe extraction through aqueous enzymatic method |
| CN103756775A (en) * | 2014-01-15 | 2014-04-30 | 盐城师范学院 | Method for extracting suaeda salsa seed oil with enzymolysis method |
| CN105132118A (en) * | 2015-09-25 | 2015-12-09 | 海南大学 | Coconut oil extraction method |
| CN106544152A (en) * | 2017-01-19 | 2017-03-29 | 合肥观云阁商贸有限公司 | From the method for extracting tea seed oil from tea seeds |
-
2017
- 2017-11-30 CN CN201711237550.5A patent/CN108587771A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102286536A (en) * | 2011-07-14 | 2011-12-21 | 同济大学 | Method for extracting cell wall combined biological demulsifiers |
| CN102796613A (en) * | 2012-08-14 | 2012-11-28 | 东北农业大学 | Demulsification method by soybean grease microbe extraction through aqueous enzymatic method |
| CN103756775A (en) * | 2014-01-15 | 2014-04-30 | 盐城师范学院 | Method for extracting suaeda salsa seed oil with enzymolysis method |
| CN105132118A (en) * | 2015-09-25 | 2015-12-09 | 海南大学 | Coconut oil extraction method |
| CN106544152A (en) * | 2017-01-19 | 2017-03-29 | 合肥观云阁商贸有限公司 | From the method for extracting tea seed oil from tea seeds |
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Application publication date: 20180928 |