CN108192719A - A kind of Suaeda salsa seed oil, crude protein and crude fibre synthesis preparation method - Google Patents
A kind of Suaeda salsa seed oil, crude protein and crude fibre synthesis preparation method Download PDFInfo
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Abstract
The present invention relates to vegetable fat extractive technique fields, more particularly to a kind of Suaeda salsa seed oil, crude protein and crude fibre synthesis preparation method, including the pretreatment of raw material ultrasound enzymolysis, supercritical extract, enzymolysis after-treatment, the preparation of composite demulsification bacterium full nutrient solution, demulsification processing, the preparation of crude protein and coarse-fibred preparation.The preparation method of the present invention can obtain three kinds of common seepweed seeds oil, crude fibre and crude protein products, while also substantially increase the oil yield of the fluffy seed of alkali simultaneously, crude protein and coarse-fibred yield.
Description
Technical field
The present invention relates to vegetable fat extractive technique fields more particularly to a kind of Suaeda salsa seed oil and albumen synthesis to prepare
Method.
Background technology
Alkali is fluffy also known as salt wormwood artemisia, seafood dish, is Chenopodiaceae annual herb salt tolerant euhalophyte, must containing human body in seed
Palpus aliphatic acid acid linoleic acid, oleic acid, leukotrienes and stearic acid, these nutritional ingredients are all the necessary objects for maintaining human body body function
Matter plays the role of body immunity before the decompression of human body hypoglycemic, expansion blood vessel, prevention and cure of cardiovascular disease and increasing etc. positive；Wherein
Linoleic acid plus linolenic acid is respectively the important metabolism production such as prostaglandin E PA (eicosapentaenoic acid), DHA (docosahexaenoic acid)
The premise of object has important role to maintaining health and adjusting physiological function.Therefore, common seepweed seeds oil is as nutrition oil
Or oil for health care is respectively provided with wide development prospect, health value is high.
At present, it is more to the preparation method research of common seepweed seeds oil, but it is fuel-displaced after grouts or residue in containing a large amount of
Crude protein and crude fibre, utilization rate is low, causes the wasting of resources；Therefore in view of the above-mentioned problems, it is necessary to establish a kind of common seepweed seeds oil,
Crude protein and the technique of crude fibre synthesis extraction.
Invention content
The technical problems to be solved by the invention are：In view of the deficienciess of the prior art, provide a kind of Suaeda salsa seed
Oil, crude protein and crude fibre synthesis preparation method can obtain common seepweed seeds oil, crude protein and thick fibre simultaneously using the preparation method
Three kinds of products are tieed up, and the oil yield of the fluffy seed of alkali is high, crude protein and coarse-fibred yield are high.
In order to solve the above technical problems, the technical scheme is that：
A kind of Suaeda salsa seed oil, crude protein and crude fibre synthesis preparation method, the preparation method include the following steps：
(1) ultrasound enzymolysis pretreatment：The Suaeda salsa seed cleaned, dried is peeled and is crushed, according to solid-liquid ratio 1:5-1:15
Ratio add in deionized water, mixing adds in complex enzyme a, ultrasound extraction, and emulsion, hydrolyzate and filter residue are collected in centrifugation respectively；
(2) supercritical extract：The filter residue of step (1) is taken, carries out supercritical carbon dioxide extracting, emulsus is collected in centrifugation respectively
Liquid, hydrolyzate and residue；
(3) after-treatment is digested：Take the residue of step (2), dries pulverizing, according to addition mixture weight 0.1-0.5%
Complex enzyme b digested, collect enzymolysis product, centrifuge and collect emulsion, hydrolyzate and residue respectively；
(4) preparation of composite demulsification bacterium full nutrient solution：Composite demulsification bacterium is taken, is culture volume 2-8% according to inoculum concentration
Ratio be inoculated on culture medium, through everfermentation, obtain composite demulsification bacterium full nutrient solution；
(5) demulsification is handled：The emulsion of step (1), (2) and (3) is merged, pH is adjusted to add in step (4) to neutrality and prepare
Demulsifying bacteria full nutrient solution, mixing carries out demulsification reaction, and the upper strata isolate that demulsification centrifuges collection after reaction is salt
Ground common seepweed seeds oil；
(6) prepared by crude protein：The hydrolyzate of step (1), (2) and step (3) is taken to merge, adjusts pH to alkalinity, centrifugation, difference
Supernatant and precipitation are collected, the precipitation of collection is washed with water, is centrifuged again, the precipitation of collection obtains crude protein by drying；
(7) prepared by crude fibre：Step (3) residue is taken, adds in deionized water, adjusting pH, microwave digestion centrifuges to alkalinity, point
The supernatant and precipitation do not collected dry supernatant to obtain water-soluble crude fibre, and precipitation drying obtains insoluble crude fibre.
As an improvement technical solution, step (1) be ultrasonically treated 40-60 DEG C of temperature, time 10-30min, power
For 300-500w.
As an improvement technical solution, the complex enzyme a in step (1) is by cellulase, lignoenzyme and glusulase
According to 0.1-0.5:0.1-0.3:The ratio of 0.15-0.3 is mixed.
As an improvement technical solution, 45-65 DEG C of the temperature of step (2) supercritical extract, extracting pressure 25-
35MPa, extraction time 1-2h.
As an improvement technical solution, the entrainer of step (2) supercritical extract is ethyl acetate or absolute ethyl alcohol.
As an improvement technical solution, complex enzyme b is pressed by papain and subtilopeptidase A in step (3)
According to 0.1-0.5:The ratio of 0.1-0.3 mixes.
As an improvement technical solution, for pH when step (3) digests for 5-7.5, temperature is 40-60 DEG C, during enzymolysis
Between be 1-2h.
As an improvement technical solution, the composite demulsification bacterium of step (4) by sludge promise cassette bacterium, demulsifying bacteria LH-1 and
Rhodococcus sp PR-1 is according to weight ratio 0.1-0.3:0.12-0.3:The ratio of 0.1-0.5 mixes.
As an improvement technical solution, step (4) ferment when fermentation condition：Shaking speed is 120-160r/
Min, cultivation temperature are 25-45 DEG C, incubation time 24-72h.
As an improvement technical solution, the demulsification temperature in step (5) is 25-30 DEG C, and the demulsification time is 0.5-2h.
After employing above-mentioned technical proposal, the beneficial effects of the invention are as follows：
Compared with prior art, the present invention mainly has the following advantages：
(1) present invention carries out ultrasound enzymolysis pretreatment by raw material of Suaeda salsa seed, by cellulase, lignoenzyme and snail
Ox enzyme can effective degradation of cell wall fraction, common seepweed seeds oil and protein component is made to enter in solvent from cell interior, is fully released
Come, into the extraction realized to common seepweed seeds oil and protein component；Grease, protein component can be extracted by supercritical extract again
More thoroughly；Enzyme is carried out by the residue that the complex enzyme that papain and subtilopeptidase A form collects supercritical extract
Solution, can promote oil component and protein component in residue to release, while reduce the bitter taste of protein component, considerably increase
The yield of albumen；Again by the composite demulsification bacterium of sludge promise cassette bacterium, demulsifying bacteria LH-1 and Rhodococcus sp PR-1 composition by emulsion
Demulsification processing is carried out, due to the lipopeptid class surface-active substance that demulsifying bacteria somatic cells generate in itself or in its metabolic process when fermenting
Matter has the ability that is relatively demulsified by force, and oil is precipitated out of emulsion, grease is promoted to release, and then substantially increase going out for the fluffy seed of alkali
Oil cut rate.The residue that secondary enzymolysis is collected simultaneously carries out microwave radiation technology alkali carries, while has obtained water-soluble crude fibre and insoluble
Crude fibre, can be used according to actual needs, greatly improve utilization rate.
(2) present invention process is simple, small toxicity, and pollution is few, and demulsification efficiency is high, and the demulsification time is short, can obtain the fluffy seed of alkali simultaneously
Three kinds of oil, crude protein and crude fibre products, and also improve oil yield, crude protein yield and water-soluble crude fibre and insoluble
The coarse-fibred yield of property.
Specific embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
Example 1
A kind of Suaeda salsa seed oil, crude protein and crude fibre synthesis preparation method, include the following steps：
(1) ultrasound enzymolysis pretreatment：The Suaeda salsa seed cleaned, dried is peeled and is crushed, according to solid-liquid ratio 1:5 ratio
Deionized water is added in, mixing adds in complex enzyme a (by cellulase, lignoenzyme and glusulase according to 0.1:0.1:0.15 ratio
Example is mixed to be formed), in 40 DEG C, power 300w of temperature, ultrasound extraction 10min, emulsion, hydrolyzate and filter are collected in centrifugation respectively
Slag；
(2) supercritical extract：The filter residue of step (1) is taken, entrainer absolute ethyl alcohol is added in, in temperature 45 C, extracting pressure
For 25MPa, supercritical carbon dioxide extracting 1h, emulsion, hydrolyzate and residue are collected in centrifugation respectively；
(3) after-treatment is digested：Take the residue of step (2), dries pulverizing, according to answering for addition mixture weight 0.15%
Synthase b is (by papain and subtilopeptidase A according to 0.1:0.15 ratio mixes) digested that (pH is
5.3, temperature is 40 DEG C, enzymolysis time 1h), enzymolysis product is collected, centrifuges and collects emulsion, hydrolyzate and residual respectively
Slag；
(4) preparation of composite demulsification bacterium full nutrient solution：Take composite demulsification bacterium (by sludge promise cassette bacterium, demulsifying bacteria LH-1 and
Rhodococcus sp PR-1 is according to weight ratio 0.1:0.15:0.1 ratio mixes), according to the ratio that inoculum concentration is culture volume 2%
Example is inoculated on culture medium, through everfermentation (shaking speed 120r/min, cultivation temperature are 25 DEG C, and incubation time is for 24 hours), is obtained
To composite demulsification bacterium full nutrient solution；
(5) demulsification is handled：The emulsion of step (1), (2) and (3) is merged, pH is adjusted to add in step (4) to neutrality and prepare
Demulsifying bacteria full nutrient solution, mixing, carry out demulsification reaction (demulsification temperature be 25 DEG C, the demulsification time be 2h), demulsification react terminates
The upper strata isolate for centrifuging collection afterwards is Suaeda salsa seed oil；
(6) prepared by crude protein：The hydrolyzate of step (1), (2) and step (3) is taken to merge, adjusts pH to alkalinity, centrifugation, difference
Supernatant and precipitation are collected, the precipitation of collection is washed with water, is centrifuged again, the precipitation of collection obtains crude protein by drying；
(7) prepared by crude fibre：Step (3) residue is taken, adds in deionized water, adjusts pH to alkalinity, microwave digestion (microwave power
For 400w, extraction time 5s, temperature is 40 DEG C), supernatant is dried to obtain by centrifugation, the supernatant and precipitation collected respectively
Water-soluble crude fibre, precipitation drying obtain insoluble crude fibre.
The oil yield that above-mentioned process conditions obtain is 24.32%, and the yield of crude protein is 22.63%, water-soluble crude fibre
Yield for 12.24%, insoluble coarse-fibred yield is 10.18%.
Example 2
A kind of Suaeda salsa seed oil, crude protein and crude fibre synthesis preparation method, include the following steps：
(1) ultrasound enzymolysis pretreatment：The Suaeda salsa seed cleaned, dried is peeled and is crushed, according to solid-liquid ratio 1:10 ratio
Example adds in deionized water, and mixing adds in complex enzyme a (by cellulase, lignoenzyme and glusulase according to 0.2:0.17:0.2
Ratio is mixed), in temperature 45 C, power 360w, ultrasound extraction 15min, emulsion, hydrolyzate and filter are collected in centrifugation respectively
Slag；
(2) supercritical extract：The filter residue of step (1) is taken, entrainer ethyl acetate is added in, in 48 DEG C of temperature, extracting pressure
For 28MPa, supercritical carbon dioxide extracting 1.2h, emulsion, hydrolyzate and residue are collected in centrifugation respectively；
(3) after-treatment is digested：Take the residue of step (2), dries pulverizing, according to answering for addition mixture weight 0.25%
Synthase b is (by papain and subtilopeptidase A according to 0.2:0.17 ratio mixes) digested that (pH is
5.8, temperature is 48 DEG C, enzymolysis time 1.3h), enzymolysis product is collected, centrifuges and collects emulsion, hydrolyzate and residual respectively
Slag；
(4) preparation of composite demulsification bacterium full nutrient solution：Take composite demulsification bacterium (by sludge promise cassette bacterium, demulsifying bacteria LH-1 and
Rhodococcus sp PR-1 is according to weight ratio 0.17:0.15:0.2 ratio mixes), it is culture volume 3.5% according to inoculum concentration
Ratio be inoculated on culture medium, through everfermentation, (shaking speed 130r/min, cultivation temperature are 32 DEG C, and incubation time is
30h), composite demulsification bacterium full nutrient solution is obtained；
(5) demulsification is handled：The emulsion of step (1), (2) and (3) is merged, pH is adjusted to add in step (4) to neutrality and prepare
Demulsifying bacteria full nutrient solution, mixing, carry out demulsification reaction (demulsification temperature be 28 DEG C, the demulsification time be 1.85h), demulsification reaction tie
The upper strata isolate that collection is centrifuged after beam is Suaeda salsa seed oil；
(6) prepared by crude protein：The hydrolyzate of step (1), (2) and step (3) is taken to merge, adjusts pH to alkalinity, centrifugation, difference
Supernatant and precipitation are collected, the precipitation of collection is washed with water, is centrifuged again, the precipitation of collection obtains crude protein by drying；
(7) prepared by crude fibre：Step (3) residue is taken, adds in deionized water, adjusts pH to alkalinity, microwave digestion (microwave digestion
Power is 420w, extraction time 8s, temperature 45 C), centrifugation, the supernatant and precipitation collected respectively dries supernatant
To water-soluble crude fibre, precipitation drying obtains insoluble crude fibre.
The oil yield that above-mentioned process conditions obtain is 24.51%, and the yield of crude protein is 22.94%, water-soluble crude fibre
Yield for 12.36%, insoluble coarse-fibred yield is 10.21%.
Example 3
A kind of Suaeda salsa seed oil, crude protein and crude fibre synthesis preparation method, include the following steps：
(1) ultrasound enzymolysis pretreatment：The Suaeda salsa seed cleaned, dried is peeled and is crushed, according to solid-liquid ratio 1:12 ratio
Example adds in deionized water, and mixing adds in complex enzyme a (by cellulase, lignoenzyme and glusulase according to 0.4:0.25:0.3
Ratio is mixed), in 58.5 DEG C, power 462w of temperature, ultrasound extraction 25min, centrifugation collect respectively emulsion, hydrolyzate and
Filter residue；
(2) supercritical extract：The filter residue of step (1) is taken, entrainer ethyl acetate is added in, in temperature 60 C, extracting pressure
For 32.5MPa, supercritical carbon dioxide extracting 1.7h, emulsion, hydrolyzate and residue are collected in centrifugation respectively；
(3) after-treatment is digested：Take the residue of step (2), dries pulverizing, according to answering for addition mixture weight 0.45%
Synthase b is (by papain and subtilopeptidase A according to 0.4:0.3 ratio mixes) digested (pH 6.8,
Temperature is 58 DEG C, enzymolysis time 1.8h), enzymolysis product is collected, centrifuges and collects emulsion, hydrolyzate and residue respectively；
(4) preparation of composite demulsification bacterium full nutrient solution：Take composite demulsification bacterium (by sludge promise cassette bacterium, demulsifying bacteria LH-1 and
Rhodococcus sp PR-1 is according to weight ratio 0.2:0.27:0.4 ratio mixes), according to the ratio that inoculum concentration is culture volume 6%
Example is inoculated on culture medium, through everfermentation (shaking speed 152r/min, cultivation temperature are 42 DEG C, incubation time 40h), is obtained
To composite demulsification bacterium full nutrient solution；
(5) demulsification is handled：The emulsion of step (1), (2) and (3) is merged, pH is adjusted to add in step (4) to neutrality and prepare
Demulsifying bacteria full nutrient solution, mixing, carry out demulsification reaction (demulsification temperature be 28.5 DEG C, the demulsification time be 0.6h), demulsification react
After centrifuge the upper strata isolate of collection as Suaeda salsa seed oil；
(6) prepared by crude protein：The hydrolyzate of step (1), (2) and step (3) is taken to merge, adjusts pH to alkalinity, centrifugation, difference
Supernatant and precipitation are collected, the precipitation of collection is washed with water, is centrifuged again, the precipitation of collection obtains crude protein by drying；
(7) prepared by crude fibre：Step (3) residue is taken, adds in deionized water, adjusts pH to alkalinity, microwave digestion (extraction power
For 450w, extraction time 10s, 48 DEG C of temperature), centrifugation, the supernatant and precipitation collected respectively dries supernatant to obtain water
Dissolubility crude fibre, precipitation drying obtain insoluble crude fibre.
The oil yield that above-mentioned process conditions obtain is 24.82%, and the yield of crude protein is 23.65%, water-soluble crude fibre
Yield for 12.42%, insoluble coarse-fibred yield is 10.26%.
Example 4
A kind of Suaeda salsa seed oil, crude protein and crude fibre synthesis preparation method, include the following steps：
(1) ultrasound enzymolysis pretreatment：The Suaeda salsa seed cleaned, dried is peeled and is crushed, according to solid-liquid ratio 1:15 ratio
Example adds in deionized water, and mixing adds in complex enzyme a (by cellulase, lignoenzyme and glusulase according to 0.5:0.3:0.3
Ratio is mixed), in temperature 60 C, power 500w, ultrasound extraction 30min, emulsion, hydrolyzate and filter are collected in centrifugation respectively
Slag；
(2) supercritical extract：The filter residue of step (1) is taken, entrainer absolute ethyl alcohol is added in, in 65 DEG C of temperature, extracting pressure
For 35MPa, supercritical carbon dioxide extracting 2h, emulsion, hydrolyzate and residue are collected in centrifugation respectively；
(3) after-treatment is digested：Take the residue of step (2), dries pulverizing, according to answering for addition mixture weight 0.5%
Synthase b is (by papain and subtilopeptidase A according to 0.5:0.3 ratio mixes) digested (pH 7.5,
Temperature is 60 DEG C, enzymolysis time 2h), enzymolysis product is collected, centrifuges and collects emulsion, hydrolyzate and residue respectively；
(4) preparation of composite demulsification bacterium full nutrient solution：Take composite demulsification bacterium (by sludge promise cassette bacterium, demulsifying bacteria LH-1 and
Rhodococcus sp PR-1 is according to weight ratio 0.3:0.3:0.5 ratio mixes), according to the ratio that inoculum concentration is culture volume 8%
Example is inoculated on culture medium, through everfermentation (shaking speed 160r/min, cultivation temperature are 45 DEG C, incubation time 72h), is obtained
To composite demulsification bacterium full nutrient solution；
(5) demulsification is handled：The emulsion of step (1), (2) and (3) is merged, pH is adjusted to add in step (4) to neutrality and prepare
Demulsifying bacteria full nutrient solution, mixing, carry out demulsification reaction (demulsification temperature be 30 DEG C, the demulsification time be 2h), demulsification react terminates
The upper strata isolate for centrifuging collection afterwards is Suaeda salsa seed oil；
(6) prepared by crude protein：The hydrolyzate of step (1), (2) and step (3) is taken to merge, adjusts pH to alkalinity, centrifugation, difference
Supernatant and precipitation are collected, the precipitation of collection is washed with water, is centrifuged again, the precipitation of collection obtains crude protein by drying；
(7) prepared by crude fibre：Step (3) residue is taken, adds in deionized water, adjusts pH to alkalinity, microwave digestion (extraction power
For 480w, extraction time 12s, 40 DEG C of temperature), centrifugation, the supernatant and precipitation collected respectively dries supernatant to obtain water
Dissolubility crude fibre, precipitation drying obtain insoluble crude fibre.
The oil yield that above-mentioned process conditions obtain is 24.67%, and the yield of albumen is 23.36%, water-soluble coarse-fibred
Yield is 12.40%, and insoluble coarse-fibred yield is 10.23%.
In order to preferably prove that Betacyanins product can be improved when extracting anthocyanidin using preparation method of the invention
Purity and recovery rate, the present invention have done 4 comparative examples simultaneously.
Comparative example 1
As different from Example 3 without carrying out demulsification processing using supercritical extract and composite demulsification bacterium, remaining condition
All same, the oil yield of gained is 22.18%, and the yield of crude protein is 22.30%, and water-soluble coarse-fibred yield is
11.89%, insoluble coarse-fibred yield is 9.87%.
Comparative example 2
As different from Example 3, demulsification processing is carried out only with demulsifying bacteria LH-1, remaining condition is identical, and gained goes out
Oil cut rate is 23.82%, and be demulsified time 2h, and the yield of crude protein is 23.10%, and water-soluble coarse-fibred yield is 12.36%, no
The coarse-fibred yield of dissolubility is 10.19%.
Comparative example 3
As different from Example 3, demulsification processing is carried out only with sludge promise cassette bacterium, remaining condition is identical, gained
Oil yield is 23.87%, and be demulsified time 1.8h, and the yield of crude protein is 23.25%, and water-soluble coarse-fibred yield is
12.38%, insoluble coarse-fibred yield is 10.21%.
Comparative example 4
As different from Example 3, demulsification processing is carried out only with Rhodococcus sp PR-1, remaining condition is identical, and gained goes out
Oil cut rate is 23.83%, and be demulsified time 1.8h, and the yield of crude protein is 23.21%, and water-soluble coarse-fibred yield is 12.39%,
Insoluble coarse-fibred yield is 10.22%.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement made within refreshing and principle etc., should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of Suaeda salsa seed oil, crude protein and crude fibre synthesis preparation method, it is characterised in that the preparation method includes
Following steps：
(1) ultrasound enzymolysis pretreatment：The Suaeda salsa seed cleaned, dried is peeled and is crushed, according to solid-liquid ratio 1:5-1:15 ratio
Example adds in deionized water, and mixing adds in complex enzyme a, ultrasound extraction, and emulsion, hydrolyzate and filter residue are collected in centrifugation respectively；
(2) supercritical extract：Take the filter residue of step (1), carry out supercritical carbon dioxide extracting, centrifugation collect respectively emulsion,
Hydrolyzate and residue；
(3) after-treatment is digested：Take the residue of step (2), dries pulverizing, according to answering for addition mixture weight 0.1-0.5%
Synthase b is digested, and collects enzymolysis product, is centrifuged and is collected emulsion, hydrolyzate and residue respectively；
(4) preparation of composite demulsification bacterium full nutrient solution：Composite demulsification bacterium is taken, according to the ratio that inoculum concentration is culture volume 2-8%
Example is inoculated on culture medium, through everfermentation, obtains composite demulsification bacterium full nutrient solution；
(5) demulsification is handled：The emulsion of step (1), (2) and (3) is merged, pH is adjusted to add in the broken of step (4) preparation to neutrality
Newborn bacterium full nutrient solution, mixing carry out demulsification reaction, and the upper strata isolate that demulsification centrifuges collection after reaction is saline land alkali
Fluffy seed oil；
(6) prepared by crude protein：The hydrolyzate of step (1), (2) and step (3) is taken to merge, pH is adjusted to be collected respectively to alkalinity, centrifugation
The precipitation of collection is washed with water, centrifuged again by supernatant and precipitation, and the precipitation of collection obtains crude protein by drying；
(7) prepared by crude fibre：Step (3) residue is taken, adds in deionized water, adjusting pH, microwave digestion is centrifuged, received respectively to alkalinity
The supernatant and precipitation of collection dry supernatant to obtain water-soluble crude fibre, and precipitation drying obtains insoluble crude fibre.
2. a kind of Suaeda salsa seed oil according to claim 1, crude protein and crude fibre synthesis preparation method, feature exist
In：40-60 DEG C of the temperature that step (1) is ultrasonically treated, time 10-30min, power 300-500w.
3. a kind of Suaeda salsa seed oil according to claim 1, crude protein and crude fibre synthesis preparation method, feature exist
In：Complex enzyme a in step (1) is by cellulase, lignoenzyme and glusulase according to 0.1-0.5:0.1-0.3:0.15-0.3
Ratio mix.
4. a kind of Suaeda salsa seed oil according to claim 1, crude protein and crude fibre synthesis preparation method, feature exist
In：45-65 DEG C of the temperature of step (2) supercritical extract, extracting pressure 25-35MPa, extraction time 1-2h.
5. a kind of Suaeda salsa seed oil according to claim 1, crude protein and crude fibre synthesis preparation method, feature exist
In：The entrainer of step (2) supercritical extract is ethyl acetate or absolute ethyl alcohol.
6. a kind of Suaeda salsa seed oil according to claim 1, crude protein and crude fibre synthesis preparation method, feature exist
In：In step (3) complex enzyme b by papain and subtilopeptidase A according to 0.1-0.5:The ratio mixing of 0.1-0.3
It forms.
7. a kind of Suaeda salsa seed oil according to claim 1, crude protein and crude fibre synthesis preparation method, feature exist
In：PH when step (3) digests is 5-7.5, and temperature is 40-60 DEG C, enzymolysis time 1-2h.
8. a kind of Suaeda salsa seed oil according to claim 1, crude protein and crude fibre synthesis preparation method, feature exist
In：The composite demulsification bacterium of step (4) is by sludge promise cassette bacterium, demulsifying bacteria LH-1 and Rhodococcus sp PR-1 according to weight ratio 0.1-0.3:
0.12-0.3:The ratio of 0.1-0.5 mixes.
9. a kind of Suaeda salsa seed oil according to claim 1, crude protein and crude fibre synthesis preparation method, feature exist
In：Fermentation condition when step (4) is fermented：Shaking speed is 120-160r/min, and cultivation temperature is 25-45 DEG C, incubation time
For 24-72h.
10. a kind of Suaeda salsa seed oil according to claim 1, crude protein and crude fibre synthesis preparation method, feature
It is：Demulsification temperature in step (5) is 25-30 DEG C, and the demulsification time is 0.5-2h.
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| CN110724593A (en) * | 2019-10-29 | 2020-01-24 | 张少清 | Method for extracting volatile oil from artemisia scoparia |
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