CN108578519A - A kind of anti-oxidant eyedrops and preparation method thereof containing jasmine tea extract - Google Patents
A kind of anti-oxidant eyedrops and preparation method thereof containing jasmine tea extract Download PDFInfo
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- CN108578519A CN108578519A CN201810318511.6A CN201810318511A CN108578519A CN 108578519 A CN108578519 A CN 108578519A CN 201810318511 A CN201810318511 A CN 201810318511A CN 108578519 A CN108578519 A CN 108578519A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/82—Theaceae (Tea family), e.g. camellia
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/63—Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/22—Heterocyclic compounds, e.g. ascorbic acid, tocopherol or pyrrolidones
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0048—Eye, e.g. artificial tears
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/08—Solutions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P27/00—Drugs for disorders of the senses
- A61P27/02—Ophthalmic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P27/00—Drugs for disorders of the senses
- A61P27/02—Ophthalmic agents
- A61P27/12—Ophthalmic agents for cataracts
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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Abstract
The invention belongs to field of deep processing of farm products, specifically provide a kind of anti-oxidant eyedrops and preparation method thereof containing jasmine tea extract.Using low-grade jasmine tea as raw material in the method, the techniques such as the extraction by different temperatures, obtain jasmine tea extract, the extract not only remains with the natural active matter in the fragrance and tealeaves of former Jasmine millet paste, and any additives are not contained, by itself and vitamin E, disodium ethylene diamine tetraacetate, the substances such as gallic acid are configured to anti-oxidant eyedrops, for mitigating, preventing or reversing the eye injury induced by oxidative stress, including the denaturation of xerophthalmia, cataract, age-related retina film etc..
Description
Technical field
The invention belongs to field of deep processing of farm products, specifically provide a kind of anti-oxidant medicament for the eyes containing jasmine tea extract
Water and preparation method thereof.
Background technology
Jasmine tea is a kind of distinctive reprocessing class tea product in Fuzhou area, it includes the mouthfeel for having tealeaves and flavour,
Also with the special fragrance of Jasmine, it can be reached by drinking jasmine tea and be refreshed the mind, soothing liver-qi stagnation alleviates eye strain
The effect of.And consumer can only achieve the purpose that enjoy jasmine tea product in such a way that boiling water brews at present, lack to jasmine
Utilization of the jasmine jasmine tea in terms of deep processing.Based on this, the present invention prepares a kind of jasmine of concentrated type by series of process method
Jasmine jasmine tea decoction extract is raw material using the extract, mixes, produce with Jasmine according to a certain percentage with other ingredients
The anti-oxidant eyedrops of tea fragrance and tea natural active material, the deodorant have the characteristics that safety, to no damage to human body, tool
There is good alleviation eye strain, prevents the function of eye disease.
Invention content
The purpose of the present invention is to provide a kind of preparation methods of the anti-oxidant eyedrops containing jasmine tea extract.This
Inventive method can prepare the deodorant for being convenient for carrying and preserving without nuisance, remain with original jasmine tea fragrance and
Tea natural product, and its it is harmless and have good prevention eye disease effect.
To achieve the above object, the present invention adopts the following technical scheme that:
(1)It is prepared by jasmine tea tealeaves
Tealeaves as raw material of the present invention derives from Theaceae Camellia(Camellia sinensis)Plant, first by bud, leaf,
After tender stem harvest, the enzymatic activity in fresh leaf is passivated by heating, then by rubbing, the azymous tealeaves that dry technology obtains,
Then Jasmine fresh flower scenting in full bloom will be added into tealeaves, weed out Jasmine again after scenting, obtain jasmine tea product.Jasmine
Grade, source, the place of production of jasmine jasmine tea etc. are unrestricted, can theoretically any tealeaves be used to carry out scenting with Jasmine.
(2)Jasmine tea millet paste extracts
Jasmine dry tea is crushed using grinding machinery, the tea powder after crushing carries out sieving processing with the sieve of 40 mesh.Tea after sieve
Powder is according to 1:30(w/w)Ratio, be added ultra-pure water.It is extracted in water-bath prior to 50-70 DEG C, 10-30min, pressure is
0.1MPa obtains low temperature leaching liquor, and it is spare that the leaching liquor is then transferred to another container memory storage.According still further to 1:30(w/w)
Ratio be added ultra-pure water, extracted in 85-100 DEG C of water-bath, 10-40 min, pressure be 0.1 MPa, obtain high-temp extracting
Liquid.
(3)Leaching liquor concentrates
Merge the liquid extracted twice, is concentrated, waited for dense with 45-55 DEG C of temperature in the Rotary Evaporators of 30-50 hPa
When contracting liquid product is the 10-20% of former leaching liquor, stop concentration.
(4)Filtering
After solution to be concentrated is cooled to room temperature, the filter membrane for being 0.22 μm with aperture is filtered, and removes insoluble matter and microorganism.
Efficient liquid phase chromatographic analysis is carried out to extracting solution obtained after filtering;
(5)The jasmine tea extract that extraction is obtained is prepared for anti-oxidant eyedrops, and the main component of the eyedrops is:
As mass fraction:It is 5%-25% jasmine teas extract, 0.005%-1% disodium ethylene diamine tetraacetates, 1%-8% vitamin Es, remaining
Amount is 0.09wt% sodium chloride solutions.
(6)Specific preparation method be by jasmine tea extract, disodium ethylene diamine tetraacetate, vitamin E after evenly mixing,
It is added the 0.09wt% sodium chloride solutions of 5 times of volumes, mixing, with the sodium bicarbonate of 0.1M or salt acid for adjusting pH value to 6.5-7.0,
It sterilizes 45 minutes at 121 DEG C, obtains eyedrops.
The advantage of the invention is that:
The present invention pointedly utilizes low temperature and high temperature extraction that can retain the leaching of Jasmine fragrance and tea natural active material
Extract, for mitigating, prevent or reverse the eye injury induced by oxidative stress, including it is xerophthalmia, cataract, age-related
Retina film denaturation etc..From the angle of cost control, low and middle-grade jasmines can be used in the jasmine tea raw material used in the present invention
Jasmine jasmine tea, derives from a wealth of sources, cheap, and broken leaf and old leaf in jasmine tealeaves processing etc. can be carried as jasmine tea
Raw material is taken, the utilization to greatest extent of resource may be implemented.
Description of the drawings
Fig. 1 is the HPLC-UV detection of each component in jasmine tea extract;
Fig. 2 is the survival rate of the retinal pigment epithelium after band-ultraviolet B irradiation;
Fig. 3 is under laser confocal microscope to different disposal cell interior peroxide observation chart;
Fig. 4 is that fluorescence microplate reader carries out quantitative detection statistics figure to different disposal Cellular peroxide levels.
Specific implementation mode
Further to disclose rather than the present invention is limited, the present invention is described in further detail below in conjunction with example.
Embodiment 1:
(1)It is prepared by jasmine tea tealeaves
Tealeaves as raw material of the present invention derives from Theaceae CamelliaCamellia sinensisPlant receives bud, leaf, tender stem
After obtaining, processing is dried in the tealeaves being collected into first, tea embryo is placed in 100-110 DEG C of heating equipment and is heated, by tea embryo
Water content is down to 4-4.5%, and by spreading for cooling, cooling, scenting is carried out when 1-3 DEG C of the tealeaves heap temperature tip high room temperature.
By the Jasmine fresh flower being collected by fraction heap, spreading for cooling makes fresh flower opening rate at 60% ~ 70%, sieve flower, and potted flower is big
It is small, reject green flower bud flower pedicle.
It first tea embryo total amount 1/3-1/4, divides on basement flower field, thickness is then to be matched according to tea, flower less than 20 cm
The fresh flower of dosage, equally separates 1/3-1/4 and uniformly spreads and be layered on tea embryo face, one layer of tea, and one layer is spent 3-5 layers alternate, then uses iron
Rake is from top to bottom pushed aside from cross section to be mixed and stirred.Tea embryo and fresh flower carry out " basement flower " when mixing by case basement, village basement, block basement method.It will
Tea, flower after split are launched in wooden case, and (46 × 43 × 43cm of wooden case specification i.e. No. two Standard China Green Tea casees) carry out case basement, per case
Basement tea amount 5kg, thickness 25-30cm.
Logical flower heat dissipation:Spreading for cooling will be pushed aside in the tea heap of basement, from the high 30-40cm of heap, the high 10cm of thin booth heap is pushed aside, every 15 points
Clock, then turn primary, offers heap tea and fully radiates, and when heap temperature reaches requirement after 1 hour, basement is answered with regard to closing heap, the high 30cm of heap, then through 5-
6 hours, tea heap temperature rose to 40 DEG C again, and flower, which has become to wither, adjusts shape, color and luster to be smelt less than fresh perfume (or spice), rocketed by turning micro- Huang in vain.
It is reached 10-12 hours in the basement time, flower, must when tea embryo absorbs moisture content and fragrance arrival certain state by devitalization
It must rocket immediately, tea and be spent separately with the machine of rocketing, that is, cry and rocket.
Tea after scenting is baked, Jasmine jasmine tea is made.
(2)Jasmine tea millet paste extracts
Jasmine dry tea is crushed using grinding machinery, the tea powder after crushing carries out sieving processing with the sieve of 40 mesh.Tea after sieve
Powder is according to 1:30(w/w)Ratio, be added ultra-pure water.It is extracted in water-bath prior to 50-70 DEG C, 10-30min, pressure is
0.1MPa obtains low temperature leaching liquor, and it is spare that the leaching liquor is then transferred to another container memory storage.According still further to 1:30(w/w)
Ratio be added ultra-pure water, extracted in 85-100 DEG C of water-bath, 10-40 min, pressure be 0.1 MPa, obtain high-temp extracting
Liquid.
(3)Leaching liquor concentrates
Merge the liquid extracted twice, is concentrated, waited for dense with 45-55 DEG C of temperature in the Rotary Evaporators of 30-50 hPa
When contracting liquid product is the 10-20% of former leaching liquor, stop concentration.
(4)Filtering
After solution to be concentrated is cooled to room temperature, the filter membrane for being 0.22 μm with aperture is filtered, and removes insoluble matter and microorganism.
Efficient liquid phase chromatographic analysis is carried out to extracting solution obtained after filtering(Fig. 1), method is with reference to GB/T 8313-2008.It is filtered
Caffeine and each catechin composition content are as shown in table 1 in liquid:
Contained caffeine and catechin in 1 filtered fluid of table(μg/mL)
GA:Gallic acid;EGC:The sub- catechin of table schema;CAF:Caffeine;C:Catechin;EC:Epicatechin;EGCG:Table does not have
Infanticide catechin and gallate;GCG:Nutgall catechin gallic acid ester;ECG:L-Epicatechin gallate;CG:Youngster
Catechin gallate.
(5)A kind of anti-oxidant eyedrops containing jasmine tea extract, by the material composition of following content:
As mass fraction:9% jasmine tea extract, 0.005%% disodium ethylene diamine tetraacetate, 5% vitamin E, surplus are
0.09wt% sodium chloride solutions.
(6)Specific preparation method be by jasmine tea extract, disodium ethylene diamine tetraacetate, vitamin E after evenly mixing,
It is added the 0.09wt% sodium chloride solutions of 5 times of volumes, mixing, with the sodium bicarbonate of 0.1M or salt acid for adjusting pH value to 6.5-7.0,
It sterilizes 45 minutes at 121 DEG C, obtains eyedrops.
Fig. 2 is illustrated after band-ultraviolet B irradiation, the survival rate of retinal pigment epithelium.
Specific cell survival rate is detected as:The MTT solution of 5 mg/mL is added into the cell of different disposal, is positioned over 37
It in DEG C incubator, is incubated by 4 h, then discards culture solution, add the dimethyl sulfoxide (DMSO) of 200 μ L, mixing is shaken, 490
Absorbance detection is carried out on nm wavelength.It is control with normal cell under the same time(100%), each to handle sample progress cell survival
Rate conversion.
After overshoot, the cell without using eyedrops of the present invention(Control)Survival rate is dropped with the extension of time
It is low.And pass through the cell using eyedrops of the present invention, it is being shown with control cell culture same time, survival rate after overshoot
It writes higher than control(p < 0.05).
Fig. 3 is the observation of intracellular peroxidation object under laser confocal microscope.Fluorescence bright spot is after being dyed by DCF-DA
Peroxide.In Fig. 3, first row is ordinary optical visual field(a1,b1,c1,d1,e1), secondary series is fluorescence visual field(a2,b2,
c2,d2,e2), third row are images after merging(a3,b3,c3,d3,e3).a1~a3:Normal cell without ultraviolet radiation.
b1~b3:After ultraviolet radiation, the cell of 30min;c1~c3:By ultraviolet radiation, and after the present invention is handled,
The cell of 30min.d1~d3:After ultraviolet radiation, the cell of 120 min;e1~e3:By ultraviolet radiation, and pass through
After crossing present invention processing, the cell of 120 min.
Fig. 4 is that fluorescence microplate reader carries out quantitative detection statistics figure to different disposal Cellular peroxide levels.Each processing group
In, the fluorescence intensity of cell interior peroxide, fluorescence intensity is higher, and the peroxide inside superficial cell is more;It is horizontal in Fig. 4
Coordinate is respectively from left to right:Ccontrol be from left to right be the normal cell without ultraviolet radiation respectively;UVB1 is warp
After crossing ultraviolet radiation, the cell of 30min;UVB2 be by ultraviolet radiation, and by the present invention handle after, 30min's
Cell;UVB3 is the cell of 120 min after ultraviolet radiation;UVB4 is by ultraviolet radiation, and the process present invention
After processing, the cell of 120 min;Ordinate is fluorescence intensity level.
The Human RPE Cells in Vitro for radiating same dose ultraviolet B radiation is subjected to fluorescent staining(DCF-DA contaminates
Color), detect, find in confocal laser scanning microscope and fluorescence intensity:Containing few in normal retina pigment epithelial cell
The free radical of amount, therefore show green under fluorescence excitation.And the cell after band-ultraviolet B irradiation, free-radical contents
Significantly rise, the quantity and fluorescence intensity of phosphor dot are all promoted.And pass through the present invention treated cell, phosphor dot
It is certain to illustrate that the present invention has the peroxide injury that ultraviolet B radiation causes all close to the range of normal cell for quantity and intensity
Inhibiting effect.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification should all belong to the covering scope of the present invention.
Claims (3)
1. a kind of anti-oxidant eyedrops containing jasmine tea extract, it is characterised in that:The main component of the eyedrops is:
As mass fraction:It is 5%-25% jasmine teas extract, 0.005%-1% disodium ethylene diamine tetraacetates, 1%-8% vitamin Es, remaining
Amount is 0.09wt% sodium chloride solutions.
2. a kind of preparing a kind of method of the anti-oxidant eyedrops containing jasmine tea extract as described in claim 1,
It is characterized in that:
After evenly mixing by jasmine tea extract, disodium ethylene diamine tetraacetate, vitamin E, the 0.09wt% of 5 times of volumes is added
Sodium chloride solution, mixing are sterilized 45 minutes at 121 DEG C, are obtained with the sodium bicarbonate of 0.1M or salt acid for adjusting pH value to 6.5-7.0
To eyedrops.
3. a kind of preparation method of anti-oxidant eyedrops containing jasmine tea extract according to claim 2, special
Sign is:The preparation specific steps of wherein jasmine tea extract include:
(1)It is prepared by jasmine tea tealeaves
Tealeaves as raw material of the present invention derives from Theaceae CamelliaCamellia sinensisPlant, first by bud, leaf, tender
After stem harvest, the enzymatic activity in fresh leaf is passivated by heating, then by rubbing, the azymous tealeaves that dry technology obtains, so
Jasmine fresh flower scenting in full bloom will be added into tealeaves afterwards, weed out Jasmine again after scenting, obtain jasmine tea product;
(2)Jasmine tea millet paste extracts
Jasmine dry tea is crushed using grinding machinery, the tea powder after crushing carries out sieving processing with the sieve of 40 mesh;Tea after sieve
Powder is according to mass ratio 1:Ultra-pure water is added in 30 ratio;It is extracted in water-bath prior to 50-70 DEG C, 10-30min, pressure is
0.1MPa obtains low temperature leaching liquor, and it is spare that the leaching liquor is then transferred to another container memory storage;According still further to mass ratio 1:30
Ratio be added ultra-pure water, extracted in 85-100 DEG C of water-bath, 10-40 min, pressure be 0.1 MPa, obtain high-temp extracting
Liquid;
(3)Leaching liquor concentrates
Merge the liquid extracted twice, is concentrated, waited for dense with 45-55 DEG C of temperature in the Rotary Evaporators of 30-50 hPa
When contracting liquid product is the 10-20% of former leaching liquor, stop concentration;
(4)Filtering
After solution to be concentrated is cooled to room temperature, the filter membrane for being 0.22 μm with aperture is filtered, and removes insoluble matter and microorganism;
Obtain jasmine tea extract.
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN113575735A (en) * | 2021-07-13 | 2021-11-02 | 广西摩氏食品科技有限公司 | Jasmine tea coffee and preparation method thereof |
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CN113575735A (en) * | 2021-07-13 | 2021-11-02 | 广西摩氏食品科技有限公司 | Jasmine tea coffee and preparation method thereof |
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