CN108576311A - A kind of method of negative pressure-ultrasonic wave added cellulase hydrolysis extraction Fu-brick tea polyphenol - Google Patents
A kind of method of negative pressure-ultrasonic wave added cellulase hydrolysis extraction Fu-brick tea polyphenol Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
- A23F3/18—Extraction of water soluble tea constituents
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Abstract
The present invention relates to a kind of methods of negative pressure ultrasound auxiliary fiber element enzymolysis and extraction Fu-brick tea polyphenol, it is mainly using water as Extraction solvent, ultrasonic cryogenic freezing processing and Vacuum Freezing ＆ Drying Technology are combined by cellulase extraction, low-temperature negative-pressure ultrasonic wave added enzymolysis, making can be mutually coordinated between each link, reduce the loss of the destruction and tea perfume odoring substance to active constituent in tealeaves, especially for the golden flower bacterium in Fu-brick tea, reduce the loss of volatile ingredient and the oxidation of tea polyphenols, the migration dissolution efficiency of tea polyphenols is improved, extraction time is shortened.
Description
Technical field
It is special standby to be related to a kind of negative pressure-ultrasonic wave added cellulase hydrolysis and carry the invention belongs to the extractive technique of Fu-brick tea polyphenol
The method for taking Fu-brick tea polyphenol.
Background technology
China is the native place of tea, and the history of tea is of long standing and well established, and tea culture is of extensive knowledge and profound scholarship, and tea industry is from simple one
Tree, leaf, cultivation processing extend to the every aspects such as history, culture, economy and society.
In recent years, change with the development of science and technology with the diversification of consumer's method of drink, tea comprehensive processing is increasingly modern
Change and refine, tea product also becomes increasingly abundant various.Instant tea is exactly a beautiful business card in tea comprehensive processing product in recent years,
For efficiently using a large amount of summer and autumn tea resource, abundant tea product, consumer facilitated to drink, improve the added value etc. of tea processing
Aspect has great importance.In the present age that rhythm of life is accelerated, instant tea has become one of favorite tea product of people,
Its solution rate is fast, without tea grounds；It drinks convenient, fast；Product saves space, merrily and lightheartedly, convenient transport；Both can add as wholefood
Add agent, but also as the main material of health protection tea based article, and can be combined very well with other extracts, therefore, before having much market
Scape.
Instant tea, be by the leaching liquor of tealeaves after concentrating, drying obtained powder product, also referred to as caffein.
Its main component is tea polysaccharide, L-Epicatechin gallate (ECG), Epigallo-catechin gallate (EGCG) (EGCG), reed
Fourth (rutin), catechin (catechin), epicatechin (epicatechin), epigallocatechin (EGC), theaflavin
The tea polyphenols substance such as (theaflavins, TFs).But there are following main problems for the research and development of instant tea：First, current institute
Extracting method tea polyphenols yield and purity are all relatively low, and reason is a lack of efficient extracting method and technology；Second is that gained
Extract tea smell is thin, mouthfeel is single, the reason is that temperature is high in extraction, concentration and drying process, the time is grown leads to fragrance object
The oxidation of a large amount of losses and part aldehydes matter of matter；Third, cold soluble poor, the millet paste muddiness of gained tea polyphenol extract object, the reason is that
Dry technology used in later stage is not advanced enough.Therefore, improving the quality of instant tea, above-mentioned there are problems to find properly it is necessary to be directed to
Solution.
The technology of extraction tea polyphenols mainly has supercritical extraction, ion catch electron microscopy, organic solvent extractionprocess, microwave at present
Extraction method, ultrasonic extraction, Enzymatic Extraction, the extraction of microwave radiation technology aqueous two-phase, ultrasound-low temperature extraction, vacuum couplings ultrasound carry
Take, conventional extraction, circumfluence distillation, cellulase synergistic supersonic wave extraction method etc., but these researchs it is mostly be only focus on acquisition compared with
High recovery rate, and the purity of extract, mouthfeel etc. are paid close attention to less.
Black brick tea is a kind of post-fermented tea of unique brick shape, is suppressed and is fermented by coarse tea leaf, main to be distributed
In provinces such as Hunan, Sichuan, Shaanxi, Yunnan.Fu-brick tea is one kind in the black tea to show unique characteristics in six major class of tealeaves, " golden flower
Bacterium " is the root of its Outstanding Quality.It is in " brick " shape, also allows for transport and the growth and breeding of " golden flower bacterium ".Fu-brick tea is earliest
As brick tea, be considered as the drink of life by the borderland people, can promoting digestion greasy, have the effect of frizing.This is also borderland people's diet
Mostly beef and mutton, but when " three high " incidence is growing in urbanite, borderland people the main reason for but health remains unchanged
One of.
For the research of Fu-brick tea focus mostly on bacterial screening, fermentation condition optimization control, golden flower bacterium metabolite regulation and control,
Millet paste physiological function etc., for further carrying out the research of deep processing using Fu-brick tea or its process leftover pieces as raw material
It is less；Simultaneously as its special brick product form is inconvenient to carry, therefore, in the present age that rhythm of life is accelerated, opened
It sends out into instant tea and has become important research direction.
In consideration of it, the present invention has studied a kind of high efficacious prescriptions of negative pressure-ultrasonic wave added cellulase hydrolysis extraction Fu-brick tea polyphenol
Method, the extract tea smell that is obtained by this method is strong, polyphenol content is high, brew is good, millet paste color and luster clear, both
It is drunk after can directly being reconstituted with water, can also reconstitute and drink together with milk.
Invention content
The object of the present invention is to provide a kind of extracting methods of Fu-brick tea polyphenol, and a kind of new deep processing is provided for Fu-brick tea
Technology.
The technical solution adopted in the present invention is：
The method of the negative pressure-ultrasonic wave added cellulase hydrolysis extraction Fu-brick tea polyphenol, comprises the steps of：
(1) selected, impurity elimination is carried out to Fu-brick tea, crushed, chosen particle of the grain size between 60~100 mesh, obtain Fu bricks
Tea powder particle；
(2) distilled water is added into the Fu-brick tea powder particles of step (1), in terms of Fu-brick tea powder particles quality, adds matter
Measure the cellulase pulvis that score is 0.8%-3.5%；
(3) it is 100-900W, frequency 20-100kHz, temperature 30- in ultrasonic power by mixed liquor obtained by step (2)
55 DEG C, vacuum degree carries out ultrasonic wave added enzymolysis and extraction 15min-3h under conditions of being 0.07-0.1MPa in extraction vessel；
(4) extracting solution obtained by step (3) is filtered, concentration is with glycerin solution under conditions of -10~-30 DEG C
Refrigerant carries out ultrasonic precooling processing, it is made quickly to form more ice crystal nucleus solid extracts, supersonic frequency 20-60kHz, work(
Rate is 100-600W；
(5) be positioned in vacuum freeze drier dry 16-30h by step (4) is extract obtained, to dried extract into
Row is crushed, screening, is classified, and packaging obtains Fu-brick tea polyphenol finished product.
It further limits, it is 1 that the solid-liquid ratio formed after distilled water is added in step (2) the Fu-brick tea powder particles:8-1:
25g/g。
It further limits, in the step (2), in terms of Fu-brick tea powder particles quality, the mass fraction of cellulase pulvis
For 1%-2%.
It further limits, in the step (3), the condition of ultrasonic wave added enzymolysis and extraction is：Ultrasonic power is 200-400W,
Frequency is 20-50kHz, and temperature is 35-45 DEG C, and vacuum degree is 0.09MPa, extraction time 30-60min in extraction vessel.
It further limits, in the step (4), the specific method of concentration is：It is 5- that aperture is crossed under 0.3-4MPa pressure
The ceramic membrane of 80nm carries out concentration.
It further limits, in the step (4), ultrasonic precooling treatment conditions are：Temperature is：- 10~-20 DEG C, ultrasound
Frequency is 20kHz, ultrasonic power 200-400W.
Further limit, the Fu-brick tea be Fu-brick tea finished product or Fu-brick tea leftover pieces.
The method of the negative pressure of the present invention-ultrasonic wave added cellulase hydrolysis extraction Fu-brick tea polyphenol, extracted by cellulase,
Low-temperature negative-pressure ultrasonic wave added enzymolysis combines ultrasonic cryogenic freezing processing and Vacuum Freezing ＆ Drying Technology, and making being capable of phase between each link
Mutually coordinate, compared with prior art, has the advantages that：
1) present invention is using low temperature, negative pressure-ultrasonic wave added enzymolysis and extraction tea polyphenols, and control low temperature is between 30-55 DEG C, area
Not in tradition extraction 70 DEG C or so of relatively-high temperature used, the destruction to active constituent in tealeaves and tea perfume can be reduced in this way
The loss of odoring substance, meanwhile, in order to achieve the effect that low temperature extraction does not reduce recovery rate, take ultrasonic wave added enzymolysis
On the one hand measure is ultrasonically treated by appropriateness and activates the activity of cellulase to improve tea polyphenols using its sonochemical effect
Migrate dissolution efficiency；On the other hand also being acted on using the physical impact of ultrasonic wave accelerates the mass transfer of tea polyphenols to spread, most final minification
Short extraction time and raising yield.Purpose using negative pressure is to make extraction mixed liquor be in approximate boiling as far as possible at low temperature to transport
Dynamic state further shortens extraction time and improves yield to further speed up mass transfer.
2) nano ceramics film is used to concentrate filtering extracting solution, it is possible to reduce the loss of volatile ingredient and tea polyphenols
Oxidation, promote tea smell and millet paste clarity.
3) present invention uses water as Extraction solvent, on the one hand reduces remaining when using ethyl alcohol, methanol etc. as solvent
Potential safety；On the other hand, the extraction of tea polyphenols is made to imitate as a result of low temperature, negative pressure-ultrasonic wave added enzymolysis and extraction technology
Rate does not reduce, and also adds the content of the nutritional ingredients such as tea polysaccharide and extraction yield in extract；It can also reduce cost simultaneously
And it is consistent so that extract is reconstituted the properties such as the millet paste nutrition after really routinely being brewed with tealeaves afterwards.
4) it uses glycerin solution as refrigerant, ultrasonic precooling processing is carried out to concentrate under cryogenic, is increased
Add the formation of tiny ice crystal core and shorten drying time in later stage, while can also further improve the brew of dry tea powder after freezing.
5) Vacuum Freezing ＆ Drying Technology is used, on the one hand is not necessarily to add the auxiliary agents such as dextrin used when spray drying, it is another
Aspect makes that products obtained therefrom tissue looseness, brew are good, millet paste color is limpid.
6) low-temperature negative-pressure ultrasonic wave added enzymolysis is combined into ultrasonic cryogenic freezing processing and Vacuum Freezing ＆ Drying Technology, mutually
Synergy substantially reduces the loss of nutritional ingredient, especially for the golden flower in Fu-brick tea while ensureing recovery rate
Bacterium, so as to get extract tea smell it is strong, polyphenol content is high, brew is good, millet paste color and luster clear, both can be direct
It is drunk after being reconstituted with water, can also reconstitute and drink together with milk.
Description of the drawings
Fig. 1 is the recovery rate comparison diagram of the lower Fu-brick tea polyphenol of various extracting conditions combination.
Fig. 2 is that different condensing modes brew millet paste color-brightness (L) comparison diagram to Fu-brick tea polyphenol extract.
Fig. 3 is the extract obtained middle polysaccharide of Different Extraction Method, polyphenol content.
Fig. 4 is influence comparison of the freezing pretreatment to extract freeze-drying powder organoleptic indicator.
Fig. 5 is influence comparison of the freezing pretreatment to the extract vacuum freeze-drying time in later stage (h).
Specific implementation mode
Technical scheme of the present invention is further described in conjunction with attached drawing and experiment, but the present invention is not limited only to down
The implementation situation stated.
The raw material Fu-brick tea of the present invention can be finished product, can also be leftover pieces in process, specific method by with
Lower step is realized：
(1) selected, impurity elimination is carried out to the finished product of Fu-brick tea or leftover pieces, crushed, cross 60 and 100 mesh screens respectively, chosen
Particle of the grain size between 60~100 mesh, obtains Fu-brick tea powder particles；
(2) distilled water, solid-liquid ratio 1 are added into the Fu-brick tea powder particles of step (1):8-25g/g, with Fu-brick tea powder
Grain quality meter adds the cellulase pulvis that mass fraction is 0.8%-3.5%；
(3) it is 100-900W, frequency 20-100kHz, temperature 30- in ultrasonic power by mixed liquor obtained by step (2)
55 DEG C, vacuum degree carries out ultrasonic wave added enzymolysis and extraction 15min-3h under conditions of being 0.07-0.1MPa in extraction vessel；
(4) extracting solution obtained by step (3) is filtered, the ceramic membrane that aperture is 5-80nm is crossed under 0.3-4MPa pressure and is carried out
Concentration carries out ultrasonic precooling processing by refrigerant of glycerin solution under conditions of -30~-10 DEG C later, makes
It quickly forms more ice crystal nucleus solid extracts, supersonic frequency 20-60kHz, power 100-600W；
(5) be positioned in vacuum freeze drier dry 16-30h by step (4) is extract obtained, to dried extract into
Row is crushed, screening, is classified, and packaging obtains Fu-brick tea polyphenol finished product.
Embodiment 1
The present embodiment is to carry out selected, impurity elimination to the leftover pieces in Fu-brick tea process, is crushed, and crosses 60 and 100 respectively
Mesh screen chooses particle of the grain size between 60~100 mesh, obtains Fu-brick tea powder particles；It is added and steams into Fu-brick tea powder particles
Distilled water, it is 1 to make solid-liquid ratio i.e. weight ratio:20g/g adds the cellulase pulvis of Fu-brick tea powder particles quality 1%；Exist later
Ultrasonic power is 400W, and frequency 25kHz, temperature is 35 DEG C, and vacuum degree is surpassed under conditions of being 0.09MPa in extraction vessel
Sound assistance enzymolysis extracts 30min；The ceramic membrane that aperture is 5-50nm is crossed under 0.3MPa pressure later and carries out concentration, later
Ultrasonic precooling processing is carried out by refrigerant of glycerin solution under conditions of -10 DEG C, it is made quickly to form more ice crystal nucleus
Solid extract, supersonic frequency 20kHz, power 400W；It is extract obtained to be positioned over drying in vacuum freeze drier
21h crushes dried extract according to conventional tea powder processing method, screens, is classified, packaging, obtain Fu-brick tea polyphenol at
Product.
Embodiment 2
The present embodiment is that distilled water is added into Fu-brick tea powder particles, and it is 1 to make solid-liquid ratio:8g/g adds Fu-brick tea powder
The cellulase pulvis of granular mass 2%；It is later 200W in ultrasonic power, frequency 20kHz, temperature is 45 DEG C, and extraction is held
Vacuum degree carries out ultrasonic wave added enzymolysis and extraction 60min under conditions of being 0.09MPa in device；Aperture is crossed under 4MPa pressure later is
The ceramic membrane of 5-80nm carries out concentration, carries out ultrasound by refrigerant of glycerin solution under conditions of -20 DEG C later
Precooling processing, makes it quickly form more ice crystal nucleus solid extracts, supersonic frequency 40kHz, power 200W；Gained extracts
Object is positioned in vacuum freeze drier dry 16h, crushes, sieves according to conventional tea powder processing method to dried extract
Choosing, classification, packaging obtain Fu-brick tea polyphenol finished product.
Embodiment 3
The present embodiment is that distilled water is added into Fu-brick tea powder particles, and it is 1 to make solid-liquid ratio:25g/g adds Fu-brick tea powder
The cellulase pulvis of granular mass 0.8%；It is later 100W in ultrasonic power, frequency 20kHz, temperature is 30 DEG C, extraction
Vacuum degree carries out ultrasonic wave added enzymolysis and extraction 3h under conditions of being 0.07MPa in container；Aperture is crossed under 1MPa pressure later is
The ceramic membrane of 5-80nm carries out concentration, carries out ultrasound by refrigerant of glycerin solution under conditions of -30 DEG C later
Precooling processing, makes it quickly form more ice crystal nucleus solid extracts, supersonic frequency 20kHz, power 100W；Gained extracts
Object is positioned in vacuum freeze drier dry 20h, crushes, sieves according to conventional tea powder processing method to dried extract
Choosing, classification, packaging obtain Fu-brick tea polyphenol finished product.
Embodiment 4
The present embodiment is that distilled water is added into Fu-brick tea powder particles, and it is 1 to make solid-liquid ratio:15g/g adds Fu-brick tea powder
The cellulase pulvis of granular mass 3.5%；It is later 900W in ultrasonic power, frequency 100kHz, temperature is 55 DEG C, extraction
Vacuum degree carries out ultrasonic wave added enzymolysis and extraction 15min under conditions of being 0.1MPa in container；Aperture is crossed under 1MPa pressure later
Concentration is carried out for the ceramic membrane of 5-80nm, is surpassed later by refrigerant of glycerin solution under conditions of -10 DEG C
The processing of sound precooling, makes it quickly form more ice crystal nucleus solid extracts, supersonic frequency 60kHz, power 600W；Gained carries
It takes object to be positioned in vacuum freeze drier dry 30h, dried extract is crushed according to conventional tea powder processing method, is sieved
Choosing, classification, packaging obtain Fu-brick tea polyphenol finished product.
In order to verify the technique effect of the present invention, now illustrated by taking following experiments as an example：
1, the recovery rate of Fu-brick tea polyphenol is compared under being combined under different extraction conditions, it is specific as follows：
1 group：35 DEG C of Extracting temperature, time 30min, solid-liquid ratio 1:Water carries under the conditions of 20 (g/g)；
2 groups：Supersound process (ultrasonic power 400W, frequency 25kHz) progress water is added on the basis of 1 group to carry；
3 groups：Cellulase (the dosage mass fraction of enzyme is 1%) progress water is added on the basis of 2 groups to carry；
4 groups：0.09MPa vacuum degrees progress water is added on the basis of 3 groups to carry.
The results are shown in Figure 1.As seen from Figure 1,1 group~4 groups of recovery rate steps up, and is carried from 1~4 group
It takes rate to be increased to 24% by 5%, thus illustrates, low temperature of the invention, negative pressure-ultrasonic wave added cellulase hydrolysis extraction combination are certain
The extraction yield of Fu-brick tea polyphenol can be greatly improved.
2, different condensing modes brew Fu-brick tea polyphenol extract the influence of millet paste color-brightness (L)
To identical extracting solution, with normal heating concentration, (it is 5-80nm's that 1MPa pressure, which descended aperture, with of the invention respectively
Ceramic membrane) concentration mode compared, and the results are shown in Figure 2, and L is the brightness of millet paste in figure, and the bigger millet paste of value is more clarified；
A* be red value of green, such as positive value and it is bigger, show that millet paste is redder, such as negative value be green；B* is champac color, is such as positive value generation
Table yellow, negative value represent blue.
As seen from Figure 2, concentration is carried out to extracting solution with the nano ceramics membrane technology of the present invention can effectively prevent
Only tea polyphenols aoxidize, and can improve the color and clarity of millet paste.
3, content influence of the different extracting modes on polysaccharide, polyphenol in extract
By conventional extracting method (35 DEG C of Extracting temperature, extraction time 30min, solid-liquid ratio 1:Under the conditions of 20 (g/g), use
Water cooks extractant and extracts) and extracting method (35 DEG C of temperature, time 30min, solid-liquid ratio 1 of the invention:20 (g/g), extraction
Ultrasonic power 400W, frequency 25kHz, the dosage mass fraction of cellulase is added as under 1%, 0.09Mpa vacuum degrees in aqueous solvent
Extraction) identical Fu-brick tea sample is extracted respectively, the results are shown in Figure 3.
As seen from Figure 3, extracting method of the invention can improve the content of tea polyphenols in extract, tea polysaccharide, make
Its total recovery rate is greatly improved, and especially the content of tea polyphenols can be the 2 times or more of general extraction methods.
4, influence of the freezing pretreatment to extract freeze-drying powder organoleptic indicator
It is carried out obtained by vacuum freeze drying after extract after the concentration of the present invention is handled through ultrasonic precooling
Sample is compared with sample obtained by vacuum freeze drying is directly carried out after concentration, and analysis pair is carried out to its organoleptic indicator
Than, subjective appreciation standard referring to the following table 1,
1 Fu-brick tea polyphenol extract subjective appreciation standard of table
Comparing result using ultrasound as shown in figure 4, as shown in Figure 4, being freezed and pre-processing its freeze-dried powder sense organ significantly better than not
Chilled pretreated extract freeze-drying powder, the obtained Fu-brick tea polyphenol extract of preparation method, entrance are through the invention
Broken, golden yellow color is pale brown, and to sepia, color is uniform, and hole wall is thin, and mellowness is good, and tea smell is strong, and brew is good, comprehensive score
91 points.
5, influence of the freezing pretreatment to the extract vacuum freeze-drying time in later stage (h)
By the present invention concentration after extract through ultrasonic precooling handle after carry out vacuum freeze drying with it is dense
The sublimation drying directly carried out used in vacuum freeze drying after contracting processing is compared, and the results are shown in Figure 5.
As seen from Figure 5, vacuum is carried out after the extract after concentration of the invention is handled through ultrasonic precooling
The freeze-drying time used is 21 hours, and directly carries out the freezing used in vacuum freeze drying in contrast experiment after concentration
Drying time is 30 hours, it is possible thereby to determine, it is cold that the present invention uses ultrasound freezing pretreatment that can be obviously shortened later stage vacuum
Dry required time is lyophilized, and ensures that nutrient loss is few while shortening sublimation drying, improves dry tea after freezing
The brew of powder.
Claims (7)
1. a kind of method of negative pressure-ultrasonic wave added cellulase hydrolysis extraction Fu-brick tea polyphenol, it is characterised in that by following steps group
At：
(1) selected, impurity elimination is carried out to Fu-brick tea, crushed, chosen particle of the grain size between 60~100 mesh, obtain Fu-brick tea powder
Particle；
(2) distilled water is added into the Fu-brick tea powder particles of step (1), in terms of Fu-brick tea powder particles quality, adds quality point
Number is the cellulase pulvis of 0.8%-3.5%；
(3) it is 100-900W in ultrasonic power by mixed liquor obtained by step (2), frequency 20-100kHz, temperature is 30-55 DEG C,
Vacuum degree carries out ultrasonic wave added enzymolysis and extraction 15min-3h under conditions of being 0.07-0.1MPa in extraction vessel；
(4) extracting solution obtained by step (3) is filtered, concentration, with glycerin solution is refrigeration under conditions of -30~-10 DEG C
Agent carries out ultrasonic precooling processing, so that it is quickly formed more ice crystal nucleus solid extracts, supersonic frequency 20-60kHz, power is
100-600W；
(5) extract obtained be positioned in vacuum freeze drier of step (4) is dried into 16-30h, powder is carried out to dried extract
Broken, screening, classification, packaging obtain Fu-brick tea polyphenol finished product.
2. the method for negative pressure according to claim 1-ultrasonic wave added cellulase hydrolysis extraction Fu-brick tea polyphenol, feature exist
It is 1 that the solid-liquid ratio formed after distilled water is added in the step (2) Fu-brick tea powder particles:8-1:25g/g.
3. the method for negative pressure according to claim 1-ultrasonic wave added cellulase hydrolysis extraction Fu-brick tea polyphenol, feature exist
In the step (2), in terms of Fu-brick tea powder particles quality, the mass fraction of cellulase pulvis is 1%-2%.
4. the method for negative pressure according to claim 1-ultrasonic wave added cellulase hydrolysis extraction Fu-brick tea polyphenol, feature exist
In the step (3), the condition of ultrasonic wave added enzymolysis and extraction is：Ultrasonic power is 200-400W, frequency 20-50kHz, temperature
Degree is 35-45 DEG C, and vacuum degree is 0.09MPa, extraction time 30-60min in extraction vessel.
5. the method for negative pressure according to claim 1-ultrasonic wave added cellulase hydrolysis extraction Fu-brick tea polyphenol, feature exist
In the step (4), the specific method of concentration is：It is dense that the ceramic membrane progress that aperture is 5-80nm is crossed under 0.3-4MPa pressure
Contracting is handled.
6. the method for negative pressure according to claim 5-ultrasonic wave added cellulase hydrolysis extraction Fu-brick tea polyphenol, feature exist
In the step (4), ultrasonic precooling treatment conditions are：Temperature is：- 10~-20 DEG C, supersonic frequency 20kHz, ultrasonic work(
Rate is 200-400W.
7. according to the method that claim 1-6 any one of them negative pressure-ultrasonic wave added cellulase hydrolysis extracts Fu-brick tea polyphenol,
It is characterized in that the Fu-brick tea be Fu-brick tea finished product or Fu-brick tea leftover pieces.
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Cited By (7)
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| CN110613033A (en) * | 2019-10-17 | 2019-12-27 | 深圳市海能达实业有限公司 | High-tea-polyphenol golden flower black tea powder extraction method and high-tea-polyphenol golden flower black tea powder |
| CN111440249A (en) * | 2020-05-19 | 2020-07-24 | 陕西科技大学 | Method for extracting Fuzhuan tea polysaccharide |
| CN112823780A (en) * | 2019-11-21 | 2021-05-21 | 湖南启农生物科技有限公司 | Extraction method of tea polyphenol |
| CN114058500A (en) * | 2020-08-03 | 2022-02-18 | 佛山市顺德区美的电热电器制造有限公司 | Control method of food processing apparatus, and storage medium |
| CN114208912A (en) * | 2021-12-16 | 2022-03-22 | 山东省农业科学院 | Extraction method and application of active ingredients in dark tea |
| CN115165509A (en) * | 2022-09-05 | 2022-10-11 | 泉州海关综合技术服务中心 | Tea water extract detection method and system |
| CN114058500B (en) * | 2020-08-03 | 2024-06-25 | 佛山市顺德区美的电热电器制造有限公司 | Control method of food processing device, food processing device and storage medium |
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| CN110613033A (en) * | 2019-10-17 | 2019-12-27 | 深圳市海能达实业有限公司 | High-tea-polyphenol golden flower black tea powder extraction method and high-tea-polyphenol golden flower black tea powder |
| CN112823780A (en) * | 2019-11-21 | 2021-05-21 | 湖南启农生物科技有限公司 | Extraction method of tea polyphenol |
| CN111440249A (en) * | 2020-05-19 | 2020-07-24 | 陕西科技大学 | Method for extracting Fuzhuan tea polysaccharide |
| CN114058500A (en) * | 2020-08-03 | 2022-02-18 | 佛山市顺德区美的电热电器制造有限公司 | Control method of food processing apparatus, and storage medium |
| CN114058500B (en) * | 2020-08-03 | 2024-06-25 | 佛山市顺德区美的电热电器制造有限公司 | Control method of food processing device, food processing device and storage medium |
| CN114208912A (en) * | 2021-12-16 | 2022-03-22 | 山东省农业科学院 | Extraction method and application of active ingredients in dark tea |
| WO2023109612A1 (en) * | 2021-12-16 | 2023-06-22 | 山东省农业科学院 | Method for extracting active ingredients in fermented tea, and application thereof |
| CN115165509A (en) * | 2022-09-05 | 2022-10-11 | 泉州海关综合技术服务中心 | Tea water extract detection method and system |
| CN115165509B (en) * | 2022-09-05 | 2022-11-11 | 泉州海关综合技术服务中心 | Tea water extract detection method and system |
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