CN112823780A - Extraction method of tea polyphenol - Google Patents
Extraction method of tea polyphenol Download PDFInfo
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- CN112823780A CN112823780A CN201911150741.7A CN201911150741A CN112823780A CN 112823780 A CN112823780 A CN 112823780A CN 201911150741 A CN201911150741 A CN 201911150741A CN 112823780 A CN112823780 A CN 112823780A
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- 150000008442 polyphenolic compounds Chemical class 0.000 title claims abstract description 53
- 235000013824 polyphenols Nutrition 0.000 title claims abstract description 53
- 238000000605 extraction Methods 0.000 title claims abstract description 37
- 241001122767 Theaceae Species 0.000 title claims abstract 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 25
- 238000000034 method Methods 0.000 claims abstract description 20
- 239000012065 filter cake Substances 0.000 claims abstract description 17
- 239000002994 raw material Substances 0.000 claims abstract description 16
- 108010059892 Cellulase Proteins 0.000 claims abstract description 14
- 229940106157 cellulase Drugs 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 238000001914 filtration Methods 0.000 claims abstract description 13
- 230000003647 oxidation Effects 0.000 abstract description 3
- 238000007254 oxidation reaction Methods 0.000 abstract description 3
- 244000269722 Thea sinensis Species 0.000 description 43
- 235000013616 tea Nutrition 0.000 description 43
- 239000000243 solution Substances 0.000 description 22
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 8
- 239000000047 product Substances 0.000 description 7
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 6
- 210000002421 cell wall Anatomy 0.000 description 6
- 238000001179 sorption measurement Methods 0.000 description 5
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
- 239000011347 resin Substances 0.000 description 4
- 229920005989 resin Polymers 0.000 description 4
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 239000002537 cosmetic Substances 0.000 description 3
- 238000004042 decolorization Methods 0.000 description 3
- 229910021645 metal ion Inorganic materials 0.000 description 3
- 239000003960 organic solvent Substances 0.000 description 3
- 238000000638 solvent extraction Methods 0.000 description 3
- 206010006326 Breath odour Diseases 0.000 description 2
- 208000032139 Halitosis Diseases 0.000 description 2
- RYYVLZVUVIJVGH-UHFFFAOYSA-N caffeine Chemical compound CN1C(=O)N(C)C(=O)C2=C1N=CN2C RYYVLZVUVIJVGH-UHFFFAOYSA-N 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000004904 shortening Methods 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 208000002064 Dental Plaque Diseases 0.000 description 1
- 201000004624 Dermatitis Diseases 0.000 description 1
- LPHGQDQBBGAPDZ-UHFFFAOYSA-N Isocaffeine Natural products CN1C(=O)N(C)C(=O)C2=C1N(C)C=N2 LPHGQDQBBGAPDZ-UHFFFAOYSA-N 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 208000012641 Pigmentation disease Diseases 0.000 description 1
- 206010040954 Skin wrinkling Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 229930014669 anthocyanidin Natural products 0.000 description 1
- 235000008758 anthocyanidins Nutrition 0.000 description 1
- 235000010208 anthocyanin Nutrition 0.000 description 1
- 229930002877 anthocyanin Natural products 0.000 description 1
- 239000004410 anthocyanin Substances 0.000 description 1
- 150000004636 anthocyanins Chemical class 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000001153 anti-wrinkle effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 229960001948 caffeine Drugs 0.000 description 1
- VJEONQKOZGKCAK-UHFFFAOYSA-N caffeine Natural products CN1C(=O)N(C)C(=O)C2=C1C=CN2C VJEONQKOZGKCAK-UHFFFAOYSA-N 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000000490 cosmetic additive Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 208000002925 dental caries Diseases 0.000 description 1
- 239000007854 depigmenting agent Substances 0.000 description 1
- YTJJRAWFHJBAMT-UHFFFAOYSA-N depside Natural products OC(=O)CC1=C(O)C=C(O)C=C1OC(=O)C1=CC=C(O)C(O)=C1 YTJJRAWFHJBAMT-UHFFFAOYSA-N 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 229930003949 flavanone Natural products 0.000 description 1
- 235000011981 flavanones Nutrition 0.000 description 1
- 150000002208 flavanones Chemical class 0.000 description 1
- HVQAJTFOCKOKIN-UHFFFAOYSA-N flavonol Natural products O1C2=CC=CC=C2C(=O)C(O)=C1C1=CC=CC=C1 HVQAJTFOCKOKIN-UHFFFAOYSA-N 0.000 description 1
- 150000002216 flavonol derivatives Chemical class 0.000 description 1
- 235000011957 flavonols Nutrition 0.000 description 1
- NWKFECICNXDNOQ-UHFFFAOYSA-N flavylium Chemical compound C1=CC=CC=C1C1=CC=C(C=CC=C2)C2=[O+]1 NWKFECICNXDNOQ-UHFFFAOYSA-N 0.000 description 1
- 235000009569 green tea Nutrition 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 150000002500 ions Chemical group 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002324 mouth wash Substances 0.000 description 1
- 229940051866 mouthwash Drugs 0.000 description 1
- 201000001245 periodontitis Diseases 0.000 description 1
- 150000007965 phenolic acids Chemical class 0.000 description 1
- 235000009048 phenolic acids Nutrition 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 239000012716 precipitator Substances 0.000 description 1
- 239000011814 protection agent Substances 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 239000002453 shampoo Substances 0.000 description 1
- 208000017520 skin disease Diseases 0.000 description 1
- 239000000344 soap Substances 0.000 description 1
- 239000000516 sunscreening agent Substances 0.000 description 1
- 238000000194 supercritical-fluid extraction Methods 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 239000000606 toothpaste Substances 0.000 description 1
- 229940034610 toothpaste Drugs 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/82—Theaceae (Tea family), e.g. camellia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/02—Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/10—Washing or bathing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/02—Preparations for cleaning the hair
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/522—Antioxidants; Radical scavengers
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/524—Preservatives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/82—Preparation or application process involves sonication or ultrasonication
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- General Chemical & Material Sciences (AREA)
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Abstract
A method for extracting tea polyphenol sequentially comprises the following steps: (1) adding water and cellulase into a raw material containing tea polyphenol, carrying out enzymolysis, and filtering to obtain a filter cake and a first extracting solution; (2) adding ethanol into the filter cake for extraction, and filtering to obtain a second extracting solution; (3) and combining the first extracting solution and the second extracting solution, and then separating to obtain the tea polyphenol. The method has short extraction time, high extraction rate, and no oxidation of tea polyphenols.
Description
Technical Field
The invention belongs to the technical field of tea polyphenol extraction.
Background
Tea polyphenols are the general name of polyphenols in tea, and are white amorphous powder, which are easily soluble in water, soluble in ethanol, methanol, acetone, ethyl acetate, and insoluble in chloroform. The green tea has high tea polyphenol content accounting for 15-30% of the weight, and the tea polyphenol comprises the following main components: flavanones, anthocyanidins, flavonols, anthocyanins, phenolic acids and depside 6 compounds.
The tea polyphenol as an excellent additive of cosmetics and daily necessities has the following remarkable effects: has strong antibacterial and oxidase inhibiting effects, and can be used for preventing and treating dermatoses, skin allergy, skin pigmentation, wrinkle, dental caries, dental plaque, periodontitis, and halitosis. The tea polyphenol can be used as antioxidant, quality guaranteeing agent, anti-wrinkle agent, skin whitening agent, radiation protection agent, sunscreen agent, special cosmetic additive and the like on cosmetics and daily necessities, and can be used on daily cosmetics such as bath soap, bath lotion, shampoo, detergent, toothpaste, tooth powder, mouthwash, anti-halitosis agent and the like to increase the functions of the products.
At present, the main extraction methods of tea polyphenol comprise the following steps: the first is organic solvent extraction: this is one of the most widely used methods. The principle is that the solubility difference of different compounds in different solvents in tea is utilized for extraction and separation, the method is simple, the extraction rate of tea polyphenol is 10-15%, and the process flow is as follows: tea → extraction with boiling water → filtration → filtrate → extraction with chloroform → extraction with ethyl acetate → concentrated drying → crude GTP; the process has the following disadvantages: a plurality of organic solvents are used in the whole production process, and the using amount of the organic solvents is large; the process is complicated, multiple times of distillation are needed, the heating time is too long, and the tea polyphenol is easy to oxidize. The other is ion precipitation extraction method, which mainly utilizes some metal ions to precipitate tea polyphenol so as to separate the tea polyphenol from caffeine. The method is characterized in that: after the tea is leached by hot water, a precipitator is added to obtain crystalline precipitates of tea polyphenol and metal ions, the leaching liquor does not need to be concentrated, the energy consumption can be reduced to a certain extent, meanwhile, because the selectivity of the precipitates is high, the purity of the product can relatively better reach more than 85 percent, but the loss of the tea polyphenol is large in the subsequent dilute acid conversion and dissolution process, and some precipitants are metal ions with certain toxicity, and some precipitants are alkaline and easily cause the oxidation of the tea polyphenol. There are also adsorption separation extraction method, supercritical fluid extraction, etc., but the techniques are not well developed.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a method for extracting tea polyphenol, which sequentially comprises the following steps:
(1) adding water and cellulase into a raw material containing tea polyphenol, carrying out enzymolysis, and filtering to obtain a filter cake and a first extracting solution;
(2) adding ethanol into the filter cake for extraction, and filtering to obtain a second extracting solution;
(3) and combining the first extracting solution and the second extracting solution, and then separating to obtain the tea polyphenol.
Wherein, the weight ratio of the cellulase to the raw material containing tea polyphenol in the step (1) is (0.5-0.9): 100; the enzymolysis temperature is normal temperature; the weight ratio of water to the raw material containing tea polyphenol is (5-8): 1. The cellulase is added in the extraction process to destroy the structure of the cell wall, so that the cell wall is locally collapsed, dissolved and loosened, thereby reducing the resistance from the cell wall and intercellular substance during solvent extraction, accelerating the speed of the effective components for dissolving out the cells, improving the extraction efficiency and shortening the extraction time.
Wherein the weight ratio of the ethanol to the filter cake in the step (2) is 3-5: 1.
Concentrating the first extract and the second extract in the step (3), and separating by using macroporous adsorption resin to obtain a pure tea polyphenol product;
more preferably, in the enzymolysis process in the step (1), ultrasonic waves are adopted for auxiliary extraction, the power is 150-.
More preferably, the first extract and the second extract are decolorized with activated carbon before being concentrated.
The invention has the following beneficial effects:
(1) the cellulase is added in the extraction process to destroy the structure of the cell wall, so that the cell wall is locally collapsed, dissolved and loosened, thereby reducing the resistance from the cell wall, the intercellular substance and the like during solvent extraction, accelerating the speed of dissolving out the effective components from the cells, shortening the extraction time and improving the extraction efficiency.
(2) Ultrasonic wave is adopted for auxiliary extraction, and the diffusion of tea polyphenol to a solvent is accelerated by utilizing the mechanical crushing and cavitation of the ultrasonic wave, so that the extraction rate of effective components is increased, and the extraction time is shortened.
(3) The cellulase and the ultrasonic wave are adopted for synchronous extraction, and normal-temperature extraction can be carried out, so that the oxidation of tea polyphenol is avoided, and the product quality is improved.
Detailed Description
The present invention is further illustrated by the following examples, which are not intended to be limiting.
Example 1
(1) Adding water and cellulase into a raw material containing tea polyphenol, carrying out enzymolysis, and filtering to obtain a filter cake and a first extracting solution;
(2) adding ethanol into the filter cake for extraction, and filtering to obtain a second extracting solution;
(3) and combining the first extracting solution and the second extracting solution, and then separating to obtain the tea polyphenol.
Wherein the weight ratio of the cellulase to the raw material containing tea polyphenol in the step (1) is 0.5: 100; the enzymolysis temperature is normal temperature; the weight ratio of the water to the raw material containing tea polyphenol is 7: 1; wherein the weight ratio of the ethanol to the filter cake in the step (2) is 4: 1; in the enzymolysis process in the step (1), ultrasonic waves are adopted for auxiliary extraction, the power is 200W, and the service time of the ultrasonic waves is 25 min.
Concentrating the first extract and the second extract in the step (3), and separating by using macroporous adsorption resin to obtain a pure tea polyphenol product; and performing activated carbon decolorization treatment before concentrating the first extracting solution and the second extracting solution.
Example 2
(1) Adding water and cellulase into a raw material containing tea polyphenol, carrying out enzymolysis, and filtering to obtain a filter cake and a first extracting solution;
(2) adding ethanol into the filter cake for extraction, and filtering to obtain a second extracting solution;
(3) and combining the first extracting solution and the second extracting solution, and then separating to obtain the tea polyphenol.
Wherein the weight ratio of the cellulase to the raw material containing tea polyphenol in the step (1) is 0.8: 100; the enzymolysis temperature is normal temperature; the weight ratio of the water to the raw material containing tea polyphenol is 7: 1; wherein the weight ratio of the ethanol to the filter cake in the step (2) is 3: 1; in the enzymolysis process in the step (1), ultrasonic waves are adopted for auxiliary extraction, the power is 150W, and the service time of the ultrasonic waves is 29 min.
Concentrating the first extract and the second extract in the step (3), and separating by using macroporous adsorption resin to obtain a pure tea polyphenol product; and performing activated carbon decolorization treatment before concentrating the first extracting solution and the second extracting solution.
Example 3
(1) Adding water and cellulase into a raw material containing tea polyphenol, carrying out enzymolysis, and filtering to obtain a filter cake and a first extracting solution;
(2) adding ethanol into the filter cake for extraction, and filtering to obtain a second extracting solution;
(3) and combining the first extracting solution and the second extracting solution, and then separating to obtain the tea polyphenol.
Wherein the weight ratio of the cellulase to the raw material containing tea polyphenol in the step (1) is 0.7: 100; the enzymolysis temperature is normal temperature; the weight ratio of the water to the raw material containing tea polyphenol is 6: 1; wherein the weight ratio of the ethanol to the filter cake in the step (2) is 5: 1; in the enzymolysis process in the step (1), ultrasonic waves are adopted for auxiliary extraction, the power is 150W, and the service time of the ultrasonic waves is 27 min.
Concentrating the first extract and the second extract in the step (3), and separating by using macroporous adsorption resin to obtain a pure tea polyphenol product; and performing activated carbon decolorization treatment before concentrating the first extracting solution and the second extracting solution.
The purity of the tea polyphenol extract obtained in the above examples 1-3 was about 98%, and the extraction rates of the extract were 29.8%, 30.2% and 28.7%, respectively.
Claims (5)
1. The extraction method of tea polyphenol is characterized by sequentially comprising the following steps:
(1) adding water and cellulase into a raw material containing tea polyphenol, carrying out enzymolysis, and filtering to obtain a filter cake and a first extracting solution;
(2) adding ethanol into the filter cake for extraction, and filtering to obtain a second extracting solution;
(3) mixing the first and second extractive solutions, and separating to obtain tea polyphenols;
wherein in the enzymolysis process in the step (1), ultrasonic waves are adopted for auxiliary extraction.
2. The method for extracting tea polyphenol as claimed in claim 1, wherein the weight ratio of the cellulase to the raw material containing tea polyphenol in step (1) is (0.5-0.9): 100; the enzymolysis temperature is normal temperature; the weight ratio of water to the raw material containing tea polyphenol is (5-8): 1.
3. The method for extracting tea polyphenol as claimed in claim 1, wherein the weight ratio of the ethanol to the filter cake in the step (2) is 3-5: 1.
4. The method as claimed in claim 1, wherein the ultrasonic power is 150-200W.
5. The method for extracting tea polyphenols according to claim 1, wherein the ultrasonic wave is used for 20-30 min.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106943768A (en) * | 2017-04-01 | 2017-07-14 | 北京国康本草物种生物科学技术研究院有限公司 | Tea Polyphenols and its extracting method and application |
| CN108576311A (en) * | 2018-03-08 | 2018-09-28 | 陕西师范大学 | A kind of method of negative pressure-ultrasonic wave added cellulase hydrolysis extraction Fu-brick tea polyphenol |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106943768A (en) * | 2017-04-01 | 2017-07-14 | 北京国康本草物种生物科学技术研究院有限公司 | Tea Polyphenols and its extracting method and application |
| CN108576311A (en) * | 2018-03-08 | 2018-09-28 | 陕西师范大学 | A kind of method of negative pressure-ultrasonic wave added cellulase hydrolysis extraction Fu-brick tea polyphenol |
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Application publication date: 20210521 |