CN108570434A - The composite bacteria agent and its manufacturing method of efficient degradation kitchen garbage - Google Patents

The composite bacteria agent and its manufacturing method of efficient degradation kitchen garbage Download PDF

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Publication number
CN108570434A
CN108570434A CN201810440896.3A CN201810440896A CN108570434A CN 108570434 A CN108570434 A CN 108570434A CN 201810440896 A CN201810440896 A CN 201810440896A CN 108570434 A CN108570434 A CN 108570434A
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China
Prior art keywords
kitchen garbage
pseudomonad
enterococcus
enterobacteria
bacteria agent
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Pending
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CN201810440896.3A
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Chinese (zh)
Inventor
庞勇
陶惠泉
张伟
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Suzhou Bo Yue Man Environmental Protection Technology Co Ltd
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Suzhou Bo Yue Man Environmental Protection Technology Co Ltd
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Priority to CN202210802777.4A priority Critical patent/CN115058368A/en
Priority to CN201810440896.3A priority patent/CN108570434A/en
Publication of CN108570434A publication Critical patent/CN108570434A/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09B—DISPOSAL OF SOLID WASTE
    • B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
    • B09B3/60—Biochemical treatment, e.g. by using enzymes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09B—DISPOSAL OF SOLID WASTE
    • B09B2101/00—Type of solid waste
    • B09B2101/70—Kitchen refuse; Food waste
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/38—Pseudomonas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/46—Streptococcus ; Enterococcus; Lactococcus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/50—Reuse, recycling or recovery technologies
    • Y02W30/82—Recycling of waste of electrical or electronic equipment [WEEE]

Abstract

The invention discloses a kind of composite bacteria agent and its manufacturing method of efficient degradation kitchen garbage, which includes pseudomonad, enterococcus, enterobacteria, and pseudomonad is aerobic bacteria, and enterococcus, enterobacteria are all facultative anaerobic bacterias.The present invention is added to two kinds of facultative anaerobic bacterias as auxiliary element, the former undertakes principal degradation work in the situation of oxygen abundance, the latter plays principal degradation work in the microenvironment for ischaemia occur, compensates for the function dead angle of the aerobic strain of single use, substantially increases garbage treatment efficiency.

Description

The composite bacteria agent and its manufacturing method of efficient degradation kitchen garbage
Technical field
The present invention relates to a kind of composite bacteria agent and its manufacturing methods, more particularly to a kind of answering for efficient degradation kitchen garbage Combined bacteria agent and its manufacturing method.
Background technology
Kitchen garbage refers to the waste material and remnants generated in the process in food process and dining.With the expansion of city size Greatly, generation increases severely, and at present to reach annual 200000000 tons or more, wherein 37%-62% is kitchen garbage.Kitchen garbage Moisture content is higher, and nutriment is abundant, easily rots in storage and transportational process and to cause acid smelly, influences urban health environment, There may be a large amount of toxin are gone out after kitchen garbage rots simultaneously, mosquitos and flies and germ are multiplied, to cause the propagation of disease, how to be closed The efficient processing kitchen garbage of reason directly affects development and the quality of life in city.Traditional Food waste treatment method includes feeding Raise livestock, landfill, burning and compost.Since non-sterile kitchen garbage carries pathogen, can be spread the disease by food chain It is detrimental to health, it is existing《People's Republic of China's animal epidemic prevention method》It has been banned from use hogwash feeding livestock.Landfill method one The diffusate of aspect land occupation resource, the putrid and deteriorated generation of another aspect kitchen garbage can polluted underground water.Burning method both consumed It is more undesirable that expense mass energy can pollute air again.Although composting method not only environmental protection waste utilization but to still remain occupancy a large amount of native again Ground simultaneously inefficiency the problem of, endlessly kitchen garbage cannot be born caused by the city size of high speed development.
Microbial garbage treatment technology is will be organic in kitchen garbage using the vital movement and metabolism of microorganism Ingredient degradation is the processing method of simple inorganic matter, is provided simultaneously with efficiently, environmental protection, energy saving three advantages.Microorganism kitchen garbage There are mainly three types of processing methods, respectively anaerobic fermentation, aerobic compost, aerobic sigestion.Wherein aerobic sigestion method is easy to use, rubbish Rubbish is reduced efficiency highest, and 90% or more organic matter transformation is vapor and innocuous gas such as CO2Deng no peculiar smell facilitates row It puts, is very suitable for focusing on a large amount of food wastes.The main microbial inoculum of aerobic sigestion method is with single aerobic strain at present Based on the composite bacteria agent of microbial inoculum or a variety of aerobic strains composition, they the shortcomings that be that oxidation process consumes a large amount of oxygen, make At local anaerobic environment, at this moment the degradation of aerobic strain receives inhibition, reduces decrement speed.
In order to solve the above problem, the present invention provides a kind of composite bacteria agent of efficient degradation kitchen garbage and its manufacturers Method is added to two kinds of facultative anaerobic bacterias as auxiliary using stringent aerobic and powerful degradation capability pseudomonad as main component Ingredient.The former undertakes principal degradation work in the situation of oxygen abundance, and the latter plays master in the microenvironment for ischaemia occur It degrades work, compensates for the function dead angle of the aerobic strain of single use, substantially increase garbage treatment efficiency.
Invention content
Technical problem to be solved by the invention is to provide a kind of composite bacteria agent of efficient degradation kitchen garbage and its manufactures Method can improve the degradation efficiency of kitchen garbage, shorten kitchen garbage processing time, reduce cost, energy saving, favorably It in the harmless treatment of kitchen garbage, reduces environmental pollution, promotes City Life Quality.
The present invention is to solve above-mentioned technical problem by following technical proposals：A kind of efficient degradation kitchen garbage is answered Combined bacteria agent and, which is characterized in that it includes pseudomonad, enterococcus, enterobacteria, and pseudomonad is aerobic bacteria, enterococcus, intestines bar Bacterium is all facultative anaerobic bacteria.
Preferably, the pseudomonad, enterococcus, enterobacteria be all from kitchen garbage screening and it is isolated.
The present invention also provides a kind of manufacturing methods of the composite bacteria agent of efficient degradation kitchen garbage, which is characterized in that it is wrapped Include following steps：
Step 1, strain recovery take out three kinds of bacterial strains from -80 DEG C of refrigerators：Pseudomonad, enterococcus, enterobacteria, 38 Being rocked in DEG C -40 DEG C of water-baths makes its fast melt；In super-clean bench, the bacterium solution that thawing is sucked out is transferred to beef extract-peptone culture It is cultivated 24 hours in constant incubator in base；It is spare that bacterium solution is stored in 4 DEG C of refrigerators；
Step 2, strain amplification take out the various bacterial strains deposited in 4 DEG C of refrigerators, with oese picking with bacteriostat A little inoculation is placed in constant temperature oscillation case the 180rpm oscillations under preference temperature in 50mL beef-protein mediums It cultivates to the growth logarithmic phase of each bacterial strain, is amplified in 500mL fluid nutrient mediums and cultivates step by step；Bacterium solution 1mL is taken out in 2000rpm Centrifugation 30 minutes removal culture medium, be resuspended after washing 2 times with 1 × PBS, using spectrophotometer measure 600nm wavelength at absorb with Detect its concentration；Three kinds of microbial inoculums are mixed in the following proportions：Pseudomonad 60-80%, enterococcus 10-20%, enterobacteria 10- 20%；
Step 3, refuse classification sort kitchen garbage, removal inorganic refuse, polybag, plastic bottle, big bone；
Step 4, composting and microbial inoculum are used by the rubbish after sorting as in reaction chamber, being heated up to 38 DEG C or so Watering can sprinkling complex micro organism fungicide is mixed with kitchen garbage, and addition microbial inoculum amount accounts for rubbish total amount 0.1-8%, turns within every 0.5 hour Turn primary, reacted 24-48 hours at 25 DEG C -40 DEG C, up to 90% or more, reaction remnants can be directly discharged into down kitchen garbage decrement Water channel.
The positive effect of the present invention is that：The microbial inoculum contains strict aerobes (pseudomonad) and amphimicrobian simultaneously Bacterium (enterococcus, enterobacteria).The composite bacteria agent is main biodegrading process degradation kitchen garbage with aerobic fermentation.Wherein pseudomonad For strict aerobes, principal degradation function is undertaken.Enterococcus and enterobacteria are facultative anaerobic bacteria, and auxiliary is played in oxygen abundance Degradation function when partial oxygen is depleted, then undertakes the degradation function of anoxic zones in a manner of anaerobic fermentation.It is facultative to detest Oxygen bacterium is to the supplement of strict aerobes, when compensating for exclusive use strict aerobes processing food garbage, in ischaemia condition The lower weakness for " reaction dead angle " occur, greatly improves degradation efficiency, reduces the garbage disposal time.It is handled through this composite bacteria agent Afterwards, the reduction rate of kitchen garbage is up to 99%.The working site free from extraneous odour that kitchen garbage is handled using this composite bacteria agent, after processing Waste liquid can direct emission.
Specific implementation mode
Present pre-ferred embodiments are given below, with the technical solution that the present invention will be described in detail.
The composite bacteria agent of efficient degradation kitchen garbage of the present invention includes pseudomonad (Pseudomonas), enterococcus (Enterococcus), enterobacteria (Enterobacter), pseudomonad are aerobic bacteria, enterococcus (Enterococcus), intestines Bacillus is all facultative anaerobic bacteria.
The production method of the composite bacteria agent of efficient degradation kitchen garbage of the present invention includes the following steps：
Step 1, strain recovery take out three kinds of bacterial strains (pseudomonad (Pseudomonas), enterococcus from -80 DEG C of refrigerators (Enterococcus), enterobacteria (Enterobacter)), being rocked in 38 DEG C of -40 DEG C of water-baths makes its fast melt.Ultra-clean In platform, be sucked out thawing bacterium solution be transferred to beef-protein medium (beef extract 3.0g, peptone 10.0g, NaCl 5.0g, Agar 15-25g, water 1000ml, high pressure sterilization) in cultivated 24 hours in constant incubator.It is standby that bacterium solution is stored in 4 DEG C of refrigerators With；
Step 2, strain amplification take out the various bacterial strains deposited in 4 DEG C of refrigerators, with oese picking with bacteriostat A little inoculation is placed in constant temperature oscillation case the 180rpm oscillations under preference temperature in 50mL beef-protein mediums It cultivates to the growth logarithmic phase of each bacterial strain, is amplified in 500mL fluid nutrient mediums and cultivates step by step.Bacterium solution 1mL is taken out in 2000rpm Centrifugation 30 minutes removal culture medium, be resuspended after washing 2 times with 1 × PBS, using spectrophotometer measure 600nm wavelength at absorb with Detect its concentration.Three kinds of microbial inoculums are mixed in the following proportions：Pseudomonad (Pseudomonas) 60-80%, enterococcus (Enterococcus) 10-20%, enterobacteria (Enterobacter) 10-20%.
Step 3, refuse classification sort kitchen garbage, removal inorganic refuse (glass, brick), polybag, modeling Expect bottle, big bone etc..
Step 4, composting and microbial inoculum are used by the rubbish after sorting as in reaction chamber, being heated up to 38 DEG C or so Watering can sprinkling complex micro organism fungicide is mixed with kitchen garbage, and addition microbial inoculum amount accounts for rubbish total amount 0.1-8%, turns within every 0.5 hour Turn primary, reacted 24-48 hours at 25 DEG C -40 DEG C, up to 90% or more, reaction remnants can be directly discharged into down kitchen garbage decrement Water channel.
Pseudomonad used herein, enterococcus, enterobacteria are all the screenings and isolated from kitchen garbage.
Case study on implementation 1
Composite bacteria agent is prepared according to the following ratio：Pseudomonad (Pseudomonas) 80%, enterococcus (Enterococcus) 10%, enterobacteria (Enterobacter) 10%.After being sorted to food garbage, added by the 0.1-8% of rubbish total amount The composite bacteria agent, overturning in every 0.5 hour is primary, keeps ventilation, is reacted 24-48 hours under 25-40 degrees Celsius, can reach makes rubbish The effect of rubbish decrement 99%.
Case study on implementation 2
Composite bacteria agent is prepared according to the following ratio：Pseudomonad (Pseudomonas) 70%, enterococcus (Enterococcus) 20%, enterobacteria (Enterobacter) 10%.After being sorted to food garbage, added by the 0.1-8% of rubbish total amount The composite bacteria agent, overturning in every 0.5 hour is primary, keeps ventilation, is reacted 24-48 hours under 25-40 degrees Celsius, can reach makes rubbish The effect of rubbish decrement 99%.
Case study on implementation 3
Composite bacteria agent is prepared according to the following ratio：Pseudomonad (Pseudomonas) 60%, enterococcus (Enterococcus) 20%, microbial inoculum is sprayed onto on solid spawn carrier and preserves by enterobacteria (Enterobacter) 20%.It is carried out to food garbage After sorting, the composite bacteria agent is added by the 0.1-8% of rubbish total amount, overturning in every 0.5 hour is primary, keeps ventilation, is taken the photograph in 25-40 It is reacted 24-48 hours under family name's degree, can reach the effect for making waste reduction 99%.
The present invention build it is a kind of can be with the strain combination reagent of efficient degradation kitchen garbage, to realize in a short period of time Harmless treatment to kitchen garbage.The invention is characterized in that：One kind is constructed to be made of strict aerobes and facultative anaerobic bacteria Composite bacteria agent.Using aerobic fermentation as major way degradation kitchen garbage, wherein strict aerobes undertakes mainly the composite bacteria agent Degradation function.Facultative anaerobic bacteria plays assistant degradation function in oxygen abundance, when partial oxygen is depleted, then with anaerobism The mode of fermentation undertakes the degradation function of anoxic zones.
The centrifuge (Xiang Yi companies, China) that the present invention uses, ultraviolet specrophotometer (Shang Yi companies, China), taper Bottle (Shang Yi companies, China), superclean bench (Suzhou purifies company, China), electro-heating standing-temperature cultivator (the upper macro company of Nereid, China), 4 DEG C of refrigerators (company of Haier, China), constant temperature oscillation case (the upper macro company of Nereid, China), vertical pressure steam sterilization pan (the rich news China in Shanghai), -80 DEG C of refrigerators (company of Haier, China), liquid-transfering gun (big dragon Xing Chuan companies, China), oese (Shanghai Bao Lu companies, China), thermostat water bath.
Particular embodiments described above, the technical issues of to the solution of the present invention, technical solution and advantageous effect carry out It is further described, it should be understood that the above is only a specific embodiment of the present invention, is not limited to The present invention, all within the spirits and principles of the present invention, any modification, equivalent substitution, improvement and etc. done should be included in this Within the protection domain of invention.

Claims (3)

1. a kind of composite bacteria agent of efficient degradation kitchen garbage, which is characterized in that it includes pseudomonad, enterococcus, enterobacteria, Pseudomonad is aerobic bacteria, and enterococcus, enterobacteria are all facultative anaerobic bacterias.
2. the composite bacteria agent of efficient degradation kitchen garbage as described in claim 1, which is characterized in that the pseudomonad, intestines Coccus, enterobacteria be all from kitchen garbage screening and it is isolated.
3. a kind of manufacturing method of the composite bacteria agent of efficient degradation kitchen garbage, which is characterized in that it includes the following steps：
Step 1, strain recovery take out three kinds of bacterial strains from -80 DEG C of refrigerators：Pseudomonad, enterococcus, enterobacteria, at 38 DEG C -40 Being rocked in DEG C water-bath makes its fast melt；In super-clean bench, the bacterium solution that thawing is sucked out is transferred in beef-protein medium It is cultivated 24 hours in constant incubator；It is spare that bacterium solution is stored in 4 DEG C of refrigerators；
Step 2, strain amplification takes out the various bacterial strains deposited in 4 DEG C of refrigerators with bacteriostat, a little with oese picking Inoculation is placed in constant temperature oscillation case the 180rpm shaken cultivations under preference temperature in 50mL beef-protein mediums To the growth logarithmic phase of each bacterial strain, it is amplified in 500mL fluid nutrient mediums and cultivates step by step；Bacterium solution 1mL is taken out to centrifuge in 2000rpm 30 minutes removal culture mediums, are resuspended after washing 2 times with 1 × PBS, are measured using spectrophotometer and are absorbed at 600nm wavelength to detect Its concentration；Three kinds of microbial inoculums are mixed in the following proportions：Pseudomonad 60-80%, enterococcus 10-20%, enterobacteria 10-20%；
Step 3, refuse classification sort kitchen garbage, removal inorganic refuse, polybag, plastic bottle, big bone；
Step 4, composting and microbial inoculum use watering can by the rubbish after sorting as in reaction chamber, being heated up to 38 DEG C or so Sprinkling complex micro organism fungicide is mixed with kitchen garbage, and addition microbial inoculum amount accounts for rubbish total amount 0.1-8%, overturning one in every 0.5 hour It is secondary, it is reacted 24-48 hours at 25 DEG C -40 DEG C, for kitchen garbage decrement up to 90% or more, reaction remnants can be directly discharged into lower water Road.
CN201810440896.3A 2018-05-10 2018-05-10 The composite bacteria agent and its manufacturing method of efficient degradation kitchen garbage Pending CN108570434A (en)

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CN201810440896.3A CN108570434A (en) 2018-05-10 2018-05-10 The composite bacteria agent and its manufacturing method of efficient degradation kitchen garbage

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