CN108566955A - A kind of anti-biogas slurry composition to eliminate aphis and its application - Google Patents
A kind of anti-biogas slurry composition to eliminate aphis and its application Download PDFInfo
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- CN108566955A CN108566955A CN201810511957.0A CN201810511957A CN108566955A CN 108566955 A CN108566955 A CN 108566955A CN 201810511957 A CN201810511957 A CN 201810511957A CN 108566955 A CN108566955 A CN 108566955A
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- China
- Prior art keywords
- biogas slurry
- imidacloprid
- eliminate aphis
- slurry composition
- composition
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N51/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds having the sequences of atoms O—N—S, X—O—S, N—N—S, O—N—N or O-halogen, regardless of the number of bonds each atom has and with no atom of these sequences forming part of a heterocyclic ring
Abstract
The invention discloses a kind of anti-biogas slurry composition to eliminate aphis, the composition includes biogas slurry and imidacloprid, and imidacloprid content in biogas slurry is 5.8 8.2mg/L.Nutrient content is in the biogas slurry：Full nitrogen 4.59g/kg, rapid available phosphorus 106.75mg/kg, available potassium 277.95mg/kg, ammonia nitrogen 0.87g/kg, rapid available phosphorus 106.75mg/kg.The pH value of the biogas slurry is 7.5 8.5.The present invention also provides a kind of above-mentioned applications for preventing the biogas slurry composition to eliminate aphis on anti-eliminate aphis.The present invention improves the virulence conspicuousness of aphid after use, reduces the use of chemical pesticide by mixing biogas slurry with Imidacloprid Insecticide, and carrying out protection to ecological environment has far reaching significance.
Description
Technical field
The invention belongs to technical field of pesticide, and in particular to a kind of anti-biogas slurry composition to eliminate aphis and its application.
Background technology
Biogas is the organic wastes such as feces of livestock and poultry by anaerobic fermentation, is a kind of emerging regenerative resource.Natural pond
Liquid is one of Fermentation Products from Biogas Generator, content various trace elements, and feces of livestock and poultry forms a large amount of microorganism alliance in fermentation process,
Increase content of organics.In recent years, marsh gas power generation factory quickly grows in China, and according to statistics, there are 3800 large-sized biogas hairs in China
Power plant has more than the quantum of output of 1,000,000,000 tons of biogas slurries every year.Biogas slurry application can increase crop yield, improve nitrogen absorbing amount and improvement
Soil texture simultaneously improves utilization rate of fertilizer, and similar to other organic soil conditioners, biogas slurry can also inhibit crop disease and insect
Occur.It can inhibit pathogenic that abundant amino acid, antagonistic microbe, plant hormone, alkanes and esters, which have, in biogas slurry
The acting factor of bacterium or desinsection.
The prevention of aphid depends on chemical broad spectrum pesticide for many years, due to frequent, a large amount of, the unreasonable use of long-time
Medicine, effect of chemical control constantly decline, and aphid has generated apparent drug resistance to a variety of insecticides.How by biogas slurry and desinsection
Agent is used in mixed way to reduce the use of chemical pesticide, increase more friendly to the control effect of aphid and to environment, symbol
The demand of social development before being fated.
Invention content
In view of this, the present invention provides a kind of anti-biogas slurry composition to eliminate aphis and its application to solve to change in the prior art
It is more to learn insecticide medication, the technical problem of preventive effect difference.
The present invention is achieved by the following technical solutions.
A kind of anti-biogas slurry composition to eliminate aphis, the composition includes biogas slurry and imidacloprid, and the imidacloprid is in biogas slurry
Content is 5.8-8.2mg/L.
Preferably, imidacloprid content in biogas slurry is 6.78mg/L.
Further, nutrient content is in the biogas slurry：Full nitrogen 4.59g/kg, rapid available phosphorus 106.75mg/kg, available potassium are
277.95mg/kg, ammonia nitrogen 0.87g/kg, rapid available phosphorus 106.75mg/kg.
Further, the pH value of the biogas slurry be 7.5-8.5, preferably 7.9.
Further, heretofore described imidacloprid is 70% water dispersible granules.
The present invention also provides the anti-biogas slurry compositions to eliminate aphis in the anti-application to eliminate aphis.
Further, the aphid is grain aphid.
The present invention contains that abundant amino acid, antagonistic microbe, plant hormone, alkanes and esters have with biogas slurry can be with
It is the acting factor for inhibiting phytopathogen or desinsection, biogas slurry is mixed with Imidacloprid Insecticide, the desinsection group being mixed to get
It closes object to improve the virulence conspicuousness of aphid, reduces the use of chemical pesticide, carrying out protection to ecological environment has far-reaching meaning
Justice.
Specific implementation mode
To keep the purpose, technical solution and effect of the application clearer, clear, below by way of specific embodiment to this hair
Bright further description.
The present invention provides a kind of anti-biogas slurry composition to eliminate aphis, and the composition includes biogas slurry and imidacloprid, the pyrrole worm
Quinoline dosage is every liter of biogas slurry dosage 5.8-8.2mg/L, preferably 6.78mg/L.Nutrient content is in the biogas slurry：Full nitrogen 4.59g/
Kg, rapid available phosphorus 106.75mg/kg, available potassium 277.95mg/kg, ammonia nitrogen 0.87g/kg, rapid available phosphorus 106.75mg/kg.
Further, the pH value of the biogas slurry be 7.5-8.5, preferably 7.9；The imidacloprid is 70% water dispersible granules.
The present invention also provides the anti-biogas slurry compositions to eliminate aphis in the anti-application to eliminate aphis, particularly for wheat long tube
The prevention of aphid.
Experiment 1：Toxicity Determination
To a kind of anti-biogas slurry composition progress Toxicity Determination to eliminate aphis provided by the invention.
1. configuration is for trying biogas slurry
It takes biogas slurry and configures its nutrient：Full nitrogen 4.59gkg-1, rapid available phosphorus 106.75mgkg-1, available potassium are
277.95mgkg-1, ammonia nitrogen 0.87gkg-1, rapid available phosphorus 106.75mgkg-1, pH value（H2O,21℃）It is 7.9.
2. cultivating selected insect source
Grain aphid is picked up from Jiangsu Inst. of Coastal Area Agricultural Sciences's barley test field of Yancheng City Tinghu District, and in room
The case where being inside not in contact with any medicament is first raised with barley.Raising temperature is 25 ± 1 DEG C, photoperiod 16/8h（L/
D）.
3. bioassay
Aphid is to the sensitivity testing of insecticide using leaching leaf dip method：Reagent agent is first diluted with water to mother liquor, then is diluted
At 5-6 concentration gradient, then by the related blade of the test worm chosen, taking-up blots medicine with blotting paper after dipping 5s in liquid together
Liquid, is put into that advance moisturizing is good to be covered in the disposable plastic culture cup of filter paper, and rim of a cup is coverd with black cloth.It is first per 30-40
Cup is repeated 3 times per concentration, and 90-120 aphids are handled per concentration.Control water process.It connects and culture cup is put into temperature after worm
It is 26 ± 1 DEG C, photoperiod 16h:8h(L：D incubator).72h inspection results after imidacloprid processing；Aphid is touched with writing brush point
Worm abdomen, foot is motionless, is considered as death.
4. data processing
Toxicity regression formula is calculated using POLO Plus softwares.Calculating parameter includes：LC50 values（The lethal concentration of 50）, LC90 values
（The required concentration of 90% individual death）.
5. experimental result
Toxicity test of 1 grain aphid of table to Imidacloprid Insecticide and biogas slurry
Wherein：The LC50 of SRa=(LC50 of the LC50 or ZY17 of DM17) ÷ MG17；The LC50 ÷ ZY17's of RRa=DM17
LC50.MG17 is sensitive strain；DM17 is to measure strain after Imidacloprid Insecticide is diluted with water；ZY17 be Imidacloprid Insecticide with
Biogas slurry is mixed to measure strain；
As it can be seen from table 1 grain aphid produces Imidacloprid Insecticide different degrees of drug resistance, imidacloprid is generated
Low-level resistance（8.5 again）.The present invention by insecticide is dissolved in sprayed in biogas slurry after find, biogas slurry and imidacloprid are mixed
It is improved with the rear virulence conspicuousness to aphid, synergic ratio reaches 4.6 times.
Experiment 2：Field efficacy is tested
Indoor result is applied to field experiment（Selection LC90 is test dose）, imidacloprid and biogas slurry are used in mixed way,
The weight of imidacloprid is 6.78mg in every liter of (L) biogas slurry；Field trial is in strict accordance with National Standard of the People's Republic of China (GB/
T17980.15-2000) it carries out.Experimental plot is located at the Nan Yang experiment of Yancheng City Tinghu District Jiangsu Inst. of Coastal Area Agricultural Sciences
Field barley test Tanaka；Experiment totally 3 processing, processing 1：Imidacloprid content in biogas slurry is 6.78mg/L；Processing 2：Imidacloprid
Content is 6.78mg/L in water；CK：Clear water compares.Each 4 repetitions of processing, per cell 20m2, cell random alignment.It uses
Instrument is 16 liters of sprayer sprayings of AGROLEX SPRAYER HD-400 profits agriculture.Liquid is equably sprayed to each position of wheat,
750 kg/hm of the liquid measure that sprays2.Before dispenser and 1 day after dispenser, the work aphid number that 3 days, 7 days investigate each cell.According to every after medicine
The secondary dead borer population (i.e. the residual borer population of insect population-before medicine) of investigation calculates each cell Revision insect recluced rate compared with insect population before medicine,
Then chemicals treatment district Revision insect recluced rate is compared with blank district and calculates correcting controling effect and corrects.
Preventive effect=[(treatment region Revision insect recluced rate-check plot Revision insect recluced rate)/(check plots 1- Revision insect recluced rate)] * 100.
Table 2：Biogas slurry and imidacloprid mixed insecticidal composition field control aphid effect
CK is expressed as clear water control in table 2, is not added with any biogas slurry and insecticide, and processing 2 is the positive controls for handling 1, by
After table 2 is as can be seen that biogas slurry and imidacloprid be used in mixed way, the preventive effect after 1,3,7 day respectively reaches 91.9%, 90.7% and 86.5%,
And imidacloprid directly spray after be respectively then 76.5%, 73.1% and 65.7% to 1,3,7 day preventive effect of aphid, illustrate biogas slurry with
Imidacloprid can preferably be prevented eliminating aphis after being combined；Therefore, from the point of view of preventive effect and ecological two angles, biogas slurry and pyrrole worm
Quinoline combination can preferably prevent field aphid and cause harm, and reduce the use of chemical pesticide, protected to ecological environment.
Claims (8)
1. a kind of anti-biogas slurry composition to eliminate aphis, it is characterised in that：The composition includes biogas slurry and imidacloprid, the imidacloprid
Content is 5.8-8.2mg/L in biogas slurry.
2. the anti-biogas slurry composition to eliminate aphis as described in claim 1, it is characterised in that：Imidacloprid content in biogas slurry
For 6.78mg/L.
3. the anti-biogas slurry composition to eliminate aphis as described in claim 1, it is characterised in that：Nutrient content is in the biogas slurry：
Full nitrogen 4.59g/kg, rapid available phosphorus 106.75mg/kg, available potassium 277.95mg/kg, ammonia nitrogen 0.87g/kg, rapid available phosphorus
106.75mg/kg。
4. the anti-biogas slurry composition to eliminate aphis as claimed in claim 3, it is characterised in that：The pH value of the biogas slurry is 7.5-
8.5。
5. the anti-biogas slurry composition to eliminate aphis as claimed in claim 4, it is characterised in that：PH value is 7.9.
6. the anti-biogas slurry composition to eliminate aphis as described in claim 1, it is characterised in that：The imidacloprid is 70% water-dispersed
Granula.
7. any one of claim 1 to the 6 anti-biogas slurry composition to eliminate aphis is in the anti-application to eliminate aphis.
8. the anti-biogas slurry composition to eliminate aphis is in the anti-application to eliminate aphis as claimed in claim 7, it is characterised in that the aphid
For grain aphid.
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| CN201810511957.0A CN108566955A (en) | 2018-05-25 | 2018-05-25 | A kind of anti-biogas slurry composition to eliminate aphis and its application |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112956486A (en) * | 2021-01-29 | 2021-06-15 | 广东省科学院生物工程研究所 | Application of nitrate nitrogen in improving imidacloprid content of plants |
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| CN102826921A (en) * | 2012-07-11 | 2012-12-19 | 大连理工大学 | Compound biogas slurry insecticidal liquid and preparation method |
| CN102934583A (en) * | 2012-11-30 | 2013-02-20 | 赖月娥 | Integrated pest and disease prevention and control management of hot peppers |
| CN103975770A (en) * | 2014-05-30 | 2014-08-13 | 刘善勇 | Edible mushroom production increasing, insect prevention and infectious microbe prevention compound |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112956486A (en) * | 2021-01-29 | 2021-06-15 | 广东省科学院生物工程研究所 | Application of nitrate nitrogen in improving imidacloprid content of plants |
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