CN108559712A - A kind of preparation method of hyacinth fermentation albumen powder - Google Patents
A kind of preparation method of hyacinth fermentation albumen powder Download PDFInfo
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- CN108559712A CN108559712A CN201711297601.3A CN201711297601A CN108559712A CN 108559712 A CN108559712 A CN 108559712A CN 201711297601 A CN201711297601 A CN 201711297601A CN 108559712 A CN108559712 A CN 108559712A
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- 239000000843 powder Substances 0.000 title claims abstract description 24
- 238000000855 fermentation Methods 0.000 title claims abstract description 16
- 230000004151 fermentation Effects 0.000 title claims abstract description 16
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 241001632576 Hyacinthus Species 0.000 title claims abstract description 12
- 239000001963 growth medium Substances 0.000 claims abstract description 26
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 21
- 239000000203 mixture Substances 0.000 claims abstract description 15
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims abstract description 14
- 235000016709 nutrition Nutrition 0.000 claims abstract description 12
- 230000035764 nutrition Effects 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 241000159512 Geotrichum Species 0.000 claims abstract description 10
- 241000894006 Bacteria Species 0.000 claims abstract description 9
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 8
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims abstract description 7
- 235000019341 magnesium sulphate Nutrition 0.000 claims abstract description 7
- 239000000463 material Substances 0.000 claims abstract description 5
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims abstract description 4
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims abstract description 4
- 235000011130 ammonium sulphate Nutrition 0.000 claims abstract description 4
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 4
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 4
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims abstract description 4
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 4
- 241000228245 Aspergillus niger Species 0.000 claims description 13
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 9
- 230000004913 activation Effects 0.000 claims description 9
- 239000008103 glucose Substances 0.000 claims description 9
- 244000168141 Geotrichum candidum Species 0.000 claims description 7
- 235000017388 Geotrichum candidum Nutrition 0.000 claims description 7
- 229920001817 Agar Polymers 0.000 claims description 6
- 244000061456 Solanum tuberosum Species 0.000 claims description 6
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 6
- 239000008272 agar Substances 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 3
- 241000235646 Cyberlindnera jadinii Species 0.000 claims description 3
- 206010013786 Dry skin Diseases 0.000 claims description 3
- 235000003239 Guizotia abyssinica Nutrition 0.000 claims description 3
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 3
- 239000001888 Peptone Substances 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
- 235000015278 beef Nutrition 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 238000003801 milling Methods 0.000 claims description 3
- 235000019319 peptone Nutrition 0.000 claims description 3
- 240000003826 Eichhornia crassipes Species 0.000 claims 2
- 241000228212 Aspergillus Species 0.000 claims 1
- 241000169203 Eichhornia Species 0.000 abstract description 24
- 238000000034 method Methods 0.000 description 8
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- 108010027322 single cell proteins Proteins 0.000 description 3
- 235000019750 Crude protein Nutrition 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 235000013325 dietary fiber Nutrition 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000004880 explosion Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005121 nitriding Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 210000004885 white matter Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses a kind of preparation methods of hyacinth fermentation albumen powder, follow these steps to carry out：(1) by weight, take 80 portions of water hyacinths under the conditions of 45 DEG C, it is 65% to be dried to water content, is crushed；(2) the water hyacinth crushed material in step (1) is added to 20 parts of wheat bran；(3) mixture in step (2) is added to 1% nutrition saline solution, the nutrition saline solution includes 0.08% ammonium sulfate, 0.12% potassium dihydrogen phosphate, magnesium sulfate 0.05%, water 1000ml；(4) 20min is heated under the conditions of the mixture in step (3) being placed in 121 DEG C to sterilize, and culture medium is made；(5) it is inoculated with according to following ratio in the mixture of step C, black-koji mould, Geotrichum, microzyme culture medium bacterium proportioning are 1:1.5:1.5,28 DEG C of temperature is closed to cultivate the characteristics of present invention was directed to water hyacinth in 72 hours, selects saccharomycete, black-koji mould and Geotrichum and ferments according to specific ratio, maximally utilizes water hyacinth resource.
Description
Technical field
The present invention relates to a kind of cultural technique fields, more particularly to a kind of preparation method of hyacinth fermentation albumen powder.
Background technology
Water hyacinth is the Strategy of Alien Invasive Species in China, belongs to one of world ten greatly evil grass, often caused by its explosion type overgrows
Ecological hazard, but it is the useful material of water body purification again, is used water hyacinth as feed resource, and domestic animal can be not only solved
The insufficient problem of feed resource of fowl, at the same also can side evil for treasured；In the existing stage, water hyacinth is mostly used as roughage,
But it exists as roughage is not easy to store, and the problem that transport is inconvenient and feed ingredient is single lacks a kind of profit in the prior art
The method that single cell protein is produced in fermentation is carried out with water hyacinth, enabling keep albumen powder made of water hyacinth full of nutrition, and
It is easy to preserve.
Therefore those skilled in the art, which are dedicated to developing, a kind of carrying out the side that single cell protein is produced in fermentation using water hyacinth
Method, enabling keep albumen powder made of water hyacinth full of nutrition, and be easy to preserve.
Invention content
In view of the drawbacks described above of the prior art, water hyacinth is utilized technical problem to be solved by the invention is to provide a kind of
Carry out the method that single cell protein is produced in fermentation, enabling keep albumen powder made of water hyacinth full of nutrition, and be easy to preserve.
To achieve the above object, the present invention provides a kind of preparation methods of hyacinth fermentation albumen powder, it is characterized in that：It presses
The following steps carry out：
(1) by weight, take 80 portions of water hyacinths under the conditions of 45 DEG C, it is 65% to be dried to water content, is crushed；
(2) the water hyacinth crushed material in step (1) is added to 20 parts of wheat bran；
(3) mixture in step (2) is added to 1% nutrition saline solution, the nutrition saline solution includes 0.08% ammonium sulfate,
0.12% potassium dihydrogen phosphate, magnesium sulfate 0.05%, water 1000ml；
(4) 20min is heated under the conditions of the mixture in step (3) being placed in 121 DEG C to sterilize, and culture medium is made；
(5) it is inoculated with according to following ratio in the mixture of step C, black-koji mould, Geotrichum, microzyme culture medium bacterium
Proportioning is 1:1.5:1.5,28 DEG C of temperature, closed culture 72 hours；
(6) by 65 DEG C of constant temperature dryings of the mixture of step D to constant weight, milling.
The technical program is directed to the characteristics of water hyacinth, passes through the strain selection to black-koji mould, Geotrichum and saccharomycete
And proportioning, albumen powder is made in hyacinth fermentation, the selection of this ingredient and proportioning can maximumlly convert water hyacinth resource, carry
High product protein content increases economy.
Preferably, in the step (5), saccharomycete uses candida utili bacterium.
Preferably, in step (5), the black-koji mould follows these steps to be prepared：
1) culture medium is prepared, using potato extracting solution 1.0L, glucose 2%, agar 1.5%, mixing, sterilize obtained training
Support base；
2) activation of aspergillus niger：It takes in culture medium of the aspergillus niger seed in step 1) and is cultivated 3 hours with 28 DEG C；
3) 8g bran mass is prepared in the bottle of 100ml, is inoculated with 10% aspergillus niger, and 28 DEG C are cultivated 3 hours, and black song is obtained
Mould.
Preferably, in step (4), the Geotrichum is prepared according to the following steps：
1) potato extracting solution 1.0L, glucose 10%, agar 8%, mixing sterilize and culture medium are made；
2) activation of geotrichum candidum：It takes in culture medium of the seed of geotrichum candidum in step 1) to cultivate 3 hours in 28 DEG C；
3) 8g bran mass is prepared in the bottle of 100ml, is inoculated with 10% aspergillus niger, and 28 DEG C are cultivated 3 hours, are obtained whitely
Mould.
Preferably, in step (4), the saccharomycete follows the steps below preparation：
1) peptone 13g, beef extract 10g, yeast powder 6g, glucose 15g, epsom salt 0.6g, water 1000ml are made
Culture medium；
2) activation of saccharomycete：It takes in culture medium of the seed of saccharomycete in step 1) and is cultivated 3 hours with 28 DEG C；
3) maltose 8g is prepared in the bottle of 100ml, is inoculated with 10% saccharomycete, 28 DEG C are cultivated 3 hours, and saccharomycete is obtained.
The beneficial effects of the invention are as follows：The present invention provides a kind of fermentation protein powder, preparation method thereofs of water hyacinth, have been directed to
The characteristics of water hyacinth, selects saccharomycete, black-koji mould and Geotrichum and ferments according to specific ratio, can maximize
Albumen conversion is carried out to water hyacinth, the product after conversion can also provide powder processed operation, can increase its economy, maximum
Change and utilizes water hyacinth resource.
Specific implementation mode
With reference to embodiment, the invention will be further described：
Embodiment：A kind of preparation method of hyacinth fermentation albumen powder, follows these steps to carry out：
(1) by weight, take 80 portions of water hyacinths under the conditions of 45 DEG C, it is 65% to be dried to water content, is crushed；
(2) the water hyacinth crushed material in step (1) is added to 20 parts of wheat bran；
(3) mixture in step (2) is added to 1% nutrition saline solution, the nutrition saline solution includes 0.08% ammonium sulfate,
0.12% potassium dihydrogen phosphate, magnesium sulfate 0.05%, water 1000ml；
(4) 20min is heated under the conditions of the mixture in step (3) being placed in 121 DEG C to sterilize, and culture medium is made；
(5) it is inoculated with according to following ratio in the mixture of step C, black-koji mould, Geotrichum, microzyme culture medium bacterium
Proportioning is 1:1.5:1.5,28 DEG C of temperature, closed culture 72 hours；
(6) by 65 DEG C of constant temperature dryings of the mixture of step D to constant weight, milling.
Particularly, in the step (5), saccharomycete uses candida utili bacterium.
Particularly, in step (5), the black-koji mould follows these steps to be prepared：1) culture medium is prepared, using horse
Bell potato extracting solution 1.0L, glucose 2%, agar 1.5%, mixing sterilize and culture medium are made；
2) activation of aspergillus niger：It takes in culture medium of the aspergillus niger seed in step 1) and is cultivated 3 hours with 28 DEG C；
3) 8g bran mass is prepared in the bottle of 100ml, is inoculated with 10% aspergillus niger, and 28 DEG C are cultivated 3 hours, and black song is obtained
Mould.
Particularly, in step (4), the Geotrichum is prepared according to the following steps：
1) potato extracting solution 1.0L, glucose 10%, agar 8%, mixing sterilize and culture medium are made；
2) activation of geotrichum candidum：It takes in culture medium of the seed of geotrichum candidum in step 1) to cultivate 3 hours in 28 DEG C；
3) 8g bran mass is prepared in the bottle of 100ml, is inoculated with 10% aspergillus niger, and 28 DEG C are cultivated 3 hours, are obtained whitely
Mould.
Particularly, in step (4), the saccharomycete follows the steps below preparation：
1) peptone 13g, beef extract 10g, yeast powder 6g, glucose 15g, epsom salt 0.6g, water 1000ml are made
Culture medium；
2) activation of saccharomycete：It takes in culture medium of the seed of saccharomycete in step 1) and is cultivated 3 hours with 28 DEG C；
3) maltose 8g is prepared in the bottle of 100ml, is inoculated with 10% saccharomycete, 28 DEG C are cultivated 3 hours, and saccharomycete is obtained.
By preceding method, water hyacinth protein feed fine powder 10KG is made, albumen powder is delivered by triumphant formula nitriding measurement
Crude protein content, while using the strong ammonium ion of Formaldehyde Absorption Method for Determination of Low, to reduce the error of crude protein measurement, through measuring, leading to
Cross aquatic albumen fine powder made from this method, protein content 42%, it follows that the albumen fine powder egg that this method generates
White matter ingredient is higher, and when as feed, abundant nutrition can be provided for animal.
The preferred embodiment of the present invention has been described in detail above.It should be appreciated that those skilled in the art without
It needs creative work according to the present invention can conceive and makes many modifications and variations.Therefore, all technologies in the art
Personnel are available by logical analysis, reasoning, or a limited experiment on the basis of existing technology under this invention's idea
Technical solution, all should be in the protection domain being defined in the patent claims.
Claims (5)
1. a kind of preparation method of hyacinth fermentation albumen powder, it is characterized in that：It follows these steps to carry out：
(1) by weight, take 80 portions of water hyacinths under the conditions of 45 DEG C, it is 65% to be dried to water content, is crushed；
(2) the water hyacinth crushed material in step (1) is added to 20 parts of wheat bran；
(3) mixture in step (2) is added to 1% nutrition saline solution, the nutrition saline solution includes 0.08% ammonium sulfate,
0.12% potassium dihydrogen phosphate, magnesium sulfate 0.05%, water 1000ml；
(4) 20min is heated under the conditions of the mixture in step (3) being placed in 121 DEG C to sterilize, and culture medium is made；
(5) it is inoculated with according to following ratio in the mixture of step C, black-koji mould, Geotrichum, microzyme culture medium bacterium proportioning
It is 1:1.5:1.5,28 DEG C of temperature, closed culture 72 hours；
(6) by 65 DEG C of constant temperature dryings of the mixture of step D to constant weight, milling.
2. a kind of preparation method of hyacinth fermentation albumen powder as described in claim 1, it is characterized in that：In the step (5),
Saccharomycete uses candida utili bacterium.
3. the preparation method of hyacinth fermentation albumen powder as described in claim 1, it is characterized in that：It is described black in step (5)
Aspergillus follows these steps to be prepared：
1) culture medium is prepared, using potato extracting solution 1.0L, glucose 2%, agar 1.5%, mixing, sterilize obtained culture
Base；
2) activation of aspergillus niger：It takes in culture medium of the aspergillus niger seed in step 1) and is cultivated 3 hours with 28 DEG C；
3) 8g bran mass is prepared in the bottle of 100ml, is inoculated with 10% aspergillus niger, and 28 DEG C are cultivated 3 hours, and aspergillus niger is obtained
Bacterium.
4. the preparation method of hyacinth fermentation albumen powder according to claim 1, it is characterized in that：It is described in step (4)
Geotrichum is prepared according to the following steps：
1) potato extracting solution 1.0L, glucose 10%, agar 8%, mixing sterilize and culture medium are made；
2) activation of geotrichum candidum：It takes in culture medium of the seed of geotrichum candidum in step 1) to cultivate 3 hours in 28 DEG C；
3) 8g bran mass is prepared in the bottle of 100ml, is inoculated with 10% aspergillus niger, and 28 DEG C are cultivated 3 hours, and geotrichum candidum is obtained
Bacterium.
5. the preparation method of hyacinth fermentation albumen powder according to claim 1, it is characterized in that：It is described in step (4)
Saccharomycete follows the steps below preparation：
1) culture is made in peptone 13g, beef extract 10g, yeast powder 6g, glucose 15g, epsom salt 0.6g, water 1000ml
Base；
2) activation of saccharomycete：It takes in culture medium of the seed of saccharomycete in step 1) and is cultivated 3 hours with 28 DEG C；
3) maltose 8g is prepared in the bottle of 100ml, is inoculated with 10% saccharomycete, 28 DEG C are cultivated 3 hours, and saccharomycete is obtained.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711297601.3A CN108559712A (en) | 2017-12-08 | 2017-12-08 | A kind of preparation method of hyacinth fermentation albumen powder |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711297601.3A CN108559712A (en) | 2017-12-08 | 2017-12-08 | A kind of preparation method of hyacinth fermentation albumen powder |
Publications (1)
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| CN108559712A true CN108559712A (en) | 2018-09-21 |
Family
ID=63529282
Family Applications (1)
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1179898A (en) * | 1997-09-26 | 1998-04-29 | 潘祥泉 | Water hyacinth fermentation powder and its producing method and its application in fodder |
| CN101744104A (en) * | 2009-12-16 | 2010-06-23 | 国家海洋局第三海洋研究所 | Preparation method of water hyacinth fermented feeds |
| CN104446814A (en) * | 2014-12-12 | 2015-03-25 | 苏州科大微龙信息技术有限公司 | Biological zinc organic leaf fertilizer and preparation method thereof |
| CN104892048A (en) * | 2015-06-02 | 2015-09-09 | 贵州汇民力生物科技有限公司 | Production process of special organic fertilizer for watermelons |
-
2017
- 2017-12-08 CN CN201711297601.3A patent/CN108559712A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1179898A (en) * | 1997-09-26 | 1998-04-29 | 潘祥泉 | Water hyacinth fermentation powder and its producing method and its application in fodder |
| CN101744104A (en) * | 2009-12-16 | 2010-06-23 | 国家海洋局第三海洋研究所 | Preparation method of water hyacinth fermented feeds |
| CN104446814A (en) * | 2014-12-12 | 2015-03-25 | 苏州科大微龙信息技术有限公司 | Biological zinc organic leaf fertilizer and preparation method thereof |
| CN104892048A (en) * | 2015-06-02 | 2015-09-09 | 贵州汇民力生物科技有限公司 | Production process of special organic fertilizer for watermelons |
Non-Patent Citations (4)
| Title |
|---|
| 赵培洁等: "《微生物发酵工艺学》", 31 October 2002, 中国环境科学出版社 * |
| 邬苏晓: "益生菌发酵凤眼莲生产蛋白质饲料的初步研究", 《饲料工业》 * |
| 雷宇杰等: "微生物发酵提高凤眼莲发酵产物蛋白含量的研究", 《信阳农业高等专科学校学报》 * |
| 黎锡流等: "《甘蔗糖厂综合利用》", 31 October 1998, 中国轻工业出版社 * |
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Application publication date: 20180921 |