CN108531519A - A kind of preparation method of S- abscisic acids - Google Patents
A kind of preparation method of S- abscisic acids Download PDFInfo
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Abstract
The present invention discloses a kind of preparation method of S abscisic acids, and the preparation method comprises the following steps：(1) zymotic fluid containing S abscisic acids is obtained by seed culture, fermented and cultured using the strain for generating S abscisic acids that can ferment；(2) by the zymotic fluid containing S abscisic acids described in step (1) through plate-frame filtering, membrane filtration, acid out, extract liquor is obtained by extraction；(3) by step (2) described extract liquor by condensing crystallizing, recrystallize, be dried to obtain purity be 95% or more S abscisic acid finished products.The preparation method can shorten fermentation period, improve the content that fermentation generates S abscisic acids, handled by Hydrolysis kinetics, avoid the waste of S abscisic acids, the purity for improving S abscisic acids simultaneously, makes the purity of S abscisic acids in final products reach 95% or more, improves product quality.In addition, preparation process condition is mild, preparation process environmental protection is conducive to the industrialization production of S abscisic acids.
Description
This application claims being submitted on 04 18th, 2017, Patent Office of the People's Republic of China, application No. is 201710254199.4, inventions
A kind of priority of the Chinese patent application of entitled " preparation method of S- abscisic acids ", entire contents are hereby incorporated by reference
In the application.
Technical field
The present invention relates to a kind of preparation methods of S- abscisic acids.
Background technology
S- abscisic acids (natural abscisic acid) are listed as five major class of plant with auxin, ethylene, gibberellin, the basic element of cell division
Spontaneous growth conditioning agent is plant " anticounter-inducer ", can start the adversity gene of plant, induced activation plant
Interior degeneration-resistant immune system, raising plant itself is to cold, arid, pest and disease damage, saline and alkaline resistance, by applying S- abscisic acids,
The usage amount that chemical pesticide can be reduced has important physiological activity and application improving many aspects such as quality of agricultural product
Value.
Currently, the preparation method of S- abscisic acids mainly has direct extraction method, chemical synthesis, three kinds of biological fermentation process.Directly
Connect extraction method refer to directly extracted from plant, but due in its plant content it is relatively low, the production method yield is relatively low, scale
It is smaller；Chemical synthesis prepares S- abscisic acids, and the purity of synthesis is relatively low, because of the product right and wrong that chemically synthesized mode obtains
Natural abscisic acid and natural abscisic acid (S- abscisic acids), only natural abscisic acid (S- abscisic acids) have more than physiological activity,
Chemical synthesis production cost is high, so only the developed countries such as Japan, U.S. are applied to extensive agricultural production at present；It is raw
S- abscisic acids yield prepared by object fermentation method is high, does not have the generation of non-natural abscisic acid, is preferable S- abscisic acids comprehensive at present
Preparation method, however there are still some technical problems for this method, for example, the existing fermentation process period is longer, output
S- abscisic acid contents need to be improved, and the by-product for generation of fermenting is more, and certain difficulty, and the mistake extracted are caused to extraction
The journey parameter waste not easy to control for causing a large amount of S- abscisic acids, in addition also because the control of the defect and procedure parameter of technique is not easy
Cause final S- abscisic acid product purities not high, only less than 95%, product quality is unable to get promotion.
Invention content
In view of this, the application provides a kind of preparation method of S- abscisic acids, fermentation period can be shortened, improve fermentation production
The content of raw S- abscisic acids, is handled by Hydrolysis kinetics, avoids the waste of S- abscisic acids, while improving the purity of S- abscisic acids,
So that the purity of S- abscisic acids in final products is reached 95% or more, improves product quality.In addition, preparation process condition is mild,
Preparation process environmental protection, is conducive to the industrialization production of S- abscisic acids.
In order to solve the above technical problems, technical solution provided by the invention is a kind of preparation method of S- abscisic acids, described
Preparation method includes the following steps：
(1) it obtains luring containing S- by seed culture, fermented and cultured using the strain for the generation S- abscisic acids that can ferment anti-
The zymotic fluid of element；
(2) by the zymotic fluid containing S- abscisic acids described in step (1) through plate-frame filtering, membrane filtration, acid out, extraction is obtained by extraction
Take liquid；
(3) by step (2) described extract liquor by condensing crystallizing, recrystallize, be dried to obtain purity be 95% or more S-
Abscisic acid finished product.
Preferably, step (1) strain that can ferment generation S- abscisic acids includes Botrytis cinerea.
Preferably, fermented and cultured described in step (1) is specially：It maintains the pH value of fermentation medium in 4-7, ferments
Culture.
Preferably, the nutrient media components of step (1) described fermented and cultured include soybean protein, lactose, glucose, malt
Sugar, maltose, sucrose, maltodextrin, starch, zein, cottonseed meal, potassium dihydrogen phosphate, epsom salt, sulfuric acid monohydrate
At least one of manganese, ferrous sulfate heptahydrate, white vitriol, ammonium sulfate, salad oil.
Wherein, the temperature of the fermented and cultured is 20-35 DEG C, and the time of fermented and cultured is 12-25d.
More preferably, the pH value of the maintenance fermentation medium is specially in 4-7：The pH value of fermentation medium is less than 4
When continuous supplementation liquid alkali；Continuous supplementation fermented and cultured base material liquid when pH value is higher than 7.
Preferably, before step (2) described plate-frame filtering, solid NaOH is added into the zymotic fluid containing S- abscisic acids
PH value is adjusted to 4-7, in the plate-frame filtering, control pressure 0.05-0.5MPa.
Preferably, step (2) described membrane filtration includes micro-filtration, ultrafiltration and nanofiltration process.
More preferably, step (2) the micro-filtration process is specially：It is 0.05-0.6MPa in pressure, filtration temperature is little
Micro-filtration is carried out under conditions of 60 DEG C.
More preferably, step (2) the ultrafiltration process is specially：It is 0.05-1.0MPa in pressure, filtration temperature is little
Ultrafiltration is carried out under conditions of 60 DEG C.
More preferably, step (2) the nanofiltration process includes level-one nanofiltration and two level nanofiltration, and the level-one nanofiltration is specific
For：It is 0.1-2.0MPa in pressure, filtration temperature carries out level-one nanofiltration under conditions of being not more than 60 DEG C；The two level nanofiltration is specific
For：It is 0.5-10.0MPa in pressure, filtration temperature carries out two level nanofiltration under conditions of being not more than 60 DEG C.
Preferably, in step (2) described extraction, the extractant used is that at least one is selected from petroleum ether, ethyl acetate, second
The pH value of acid butyl ester, n-amyl alcohol, ethyl alcohol, acetone, methanol, extraction system is 1-4.
Preferably, step (3) condensing crystallizing, recrystallization, drying temperature be 40-80 DEG C.
The purity of the S- abscisic acids is the purity being calculated in mass percent.
Technical scheme provides a kind of preparation method of S- abscisic acids, and specifically a kind of fermentation method produces weight hundred
The preparation method for the S- abscisic acids that score is 95% or more produces S- abscisic acids using fermentation method, and yield is higher, by from hair
Hydrolysis kinetics in zymotic fluid, obtained S- abscisic acid products have 95% or more purity.
In above-mentioned technical scheme, in step (1), using that can ferment, the strain for generating S- abscisic acids passes through
Seed culture, fermented and cultured obtain the zymotic fluid containing S- abscisic acids, wherein the strain for the generation S- abscisic acids that can ferment is selected from,
In fermentation medium, with glucose, lactose, maltose, maltose, sucrose, maltodextrin, starch, soybean protein, zein,
At least one of cottonseed meal improves the ratio of beneficial element as carbon source and nitrogen source using ammonium sulfate as nitrogen source, promotes
Strain fermentation promotes quickly generating for S- abscisic acids；The natural oil salad oil contained makes culture medium feed liquid as carbon source
It is sticky, there is defoaming effect, and prevent from being contaminated during fermented and cultured；It is added to the micro member for promoting strain quickly to breed
Plain manganese, is added in the form of manganese sulfate monohydrate；Epsom salt, ferrous sulfate heptahydrate, white vitriol are also added, is increased
Add magnesium elements, ferro element, the Zn-ef ficiency for promoting strain fermentation process in fermented and cultured, promotes the generation of S- abscisic acids.In addition,
Continuous supplementation supplements liquid alkali when pH value is less than 4 in fermentation medium；Continuous supplementation fermented and cultured base material liquid when pH value is higher than 7,
The pH value of the fermentation medium is set to maintain 4-7.By way of flowing liquid feeding state alkali and fermented and cultured base material liquid, fermentation is trained
The pH value for supporting base is finely adjusted, and will not both be changed the ingredient of fermentation medium, will not be interfered to the fermentation of strain.Pass through
Suitable for producing culture medium setting and the combination of condition of culture of S- abscisic acid strains, fermentation period can be obviously shortened (from 29 days
Foreshorten to 18 days), while improving the content (being increased to 8000ppm from 3000ppm) of S- abscisic acids in zymotic fluid.
In step (2), by the zymotic fluid containing S- abscisic acids through plate-frame filtering, membrane filtration, acid out, extraction is obtained by extraction
Liquid.Wherein before plate-frame filtering, solid NaOH is first added and adjusts pH value to 4-7, plays filtrating aid function；In addition, passing through film mistake
The filtrate after plate-frame filtering is further filtered in filter, which includes the micro-filtration set gradually, ultrafiltration and nanofiltration, in micro-filtration
In the process, the suspended particulate in the filtrate after plate-frame filtering, strain and other large granular impurities are filtered out；In ultra-filtration process
In, the macro-molecular protein in filtrate, glucose after micro-filtration etc. are filtered out；In nanofiltration process, to the filtrate after ultrafiltration into
One step filters, and filters out part small molecule, obtains more pure S- abscisic acid solution.Control micro-filtration, ultrafiltration and nanofiltration process
In pressure, the efficiency of filtering can be improved, while avoiding the waste of filtrate.Solution after membrane filtration is carried out by extracting
Further separation, using the conduct of at least one of petroleum ether, ethyl acetate, butyl acetate, n-amyl alcohol, ethyl alcohol, acetone, methanol
Extractant, the system for controlling extraction are the acidic environment that pH value is 1-4, realize the separation of S- abscisic acids and oils, Ester.
By above filtering and extraction process, the content of S- abscisic acids in solution is improved.
In step (3), it is 95% or more that extract liquor can obtain weight percent by condensing crystallizing, recrystallization, drying
S- abscisic acid finished products.In condensing crystallizing, recrystallization process, obtain S- abscisic acid crystalline solid, make its in extract liquor can
The separation of molten inorganic salts, improves its purity, control condensing crystallizing, recrystallization, drying temperature between 40-80 DEG C, can be with
Accelerate condensing crystallizing, recrystallization, drying speed, while the structure to S- abscisic acids being avoided to damage, influences the work of its physiology
Property.
Compared with prior art, the preparation method of a kind of S- abscisic acids described in technical scheme, can shorten hair
The ferment period improves the content that fermentation generates S- abscisic acids, is handled by Hydrolysis kinetics, can further increase the pure of S- abscisic acids
Degree, makes the purity of S- abscisic acids in final products reach 95% or more.In addition, preparation process condition is mild, preparation process environmental protection,
Be conducive to the industrialization production of S- abscisic acids.
Specific implementation mode
In order to make those skilled in the art more fully understand technical scheme of the present invention, with reference to specific embodiment pair
The present invention is described in further detail.
A kind of preparation method of S- abscisic acids described herein specifically uses fermentation method production purity for 95% or more
Preparation method, include following steps：
(1) it obtains luring containing S- by seed culture, fermented and cultured using the strain for the generation S- abscisic acids that can ferment anti-
The zymotic fluid of element；Wherein, can ferment generate S- abscisic acids strain include Botrytis cinerea, the culture medium of fermented and cultured
Component includes：Soybean protein, lactose, glucose, maltose, maltose, sucrose, maltodextrin, starch, zein, cottonseed cake
Powder, potassium dihydrogen phosphate, epsom salt, manganese sulfate monohydrate, ferrous sulfate heptahydrate, white vitriol, ammonium sulfate, salad oil；Institute
The temperature for stating fermented and cultured is 20-35 DEG C, and the time of fermented and cultured is 12-25d.In fermented and cultured, pH in fermentation medium
Continuous supplementation liquid alkali when value is less than 4；Continuous supplementation fermented and cultured base material liquid when pH value is higher than 7, makes the fermentation medium
PH value maintains 4-7, and wherein liquid nitrogen source can be ammonium hydroxide.
(2) by the zymotic fluid containing S- abscisic acids described in step (1) through plate-frame filtering, membrane filtration, acid out, extraction is obtained by extraction
Take liquid, wherein before plate-frame filtering, solid NaOH is added into the zymotic fluid containing S- abscisic acids and adjusts pH value to 4-7, institute
It states in plate-frame filtering, control pressure 0.05-0.5MPa；Membrane filtration includes the micro-filtration set gradually, ultrafiltration and nanofiltration process,
In micro-filtration process, pressure 0.05-0.6MPa, filtration temperature is not more than 60 DEG C；In ultrafiltration process, pressure 0.05-
1.0MPa, filtration temperature are not more than 60 DEG C；Nanofiltration process includes level-one nanofiltration and two level nanofiltration, in the level-one nanofiltration, pressure
For 0.1-2.0MPa, filtration temperature is not more than 60 DEG C；In the two level nanofiltration, pressure 0.5-10.0MPa, filtration temperature is little
In 60 DEG C.In extraction process, extractant be it is at least one selected from petroleum ether, ethyl acetate, butyl acetate, n-amyl alcohol, ethyl alcohol,
The pH value of acetone, methanol, extraction system is 1-4.
(3) by step (2) described extract liquor by a temperature of 40-80 DEG C condensing crystallizing, recrystallize, be dried to obtain it is described
S- abscisic acid finished products.
In order to verify the technique effect of technical scheme, on the basis of above-mentioned specific implementation mode requires, use
Design parameter carries out verification experimental verification, obtains following specific examples.
Embodiment 1
Influence of the fermentation medium to fermentation period and S- abscisic acid yield
Preparation method is：
(1) by Botrytis cinerea (Botrytis Cinerea-Cercospoxa Rosicola FD338, abbreviation
B.C.FD338) pass through seed culture, fermented and cultured obtains the zymotic fluid containing S- abscisic acids.
Wherein, the culture medium composition and fermentation period of fermented and cultured are as shown in table 1.Fermentation temperature is 20~35 DEG C.
Continuous supplementation supplements ammonium hydroxide when pH value is less than 4 in the fermentation medium；Continuous supplementation fermented and cultured when pH value is higher than 7
Base material liquid makes the pH value of the fermentation medium maintain 4.5.
(2) by the zymotic fluid containing S- abscisic acids that step (1) obtains through plate-frame filtering, membrane filtration, acid out, be obtained by extraction
Extract liquor.
Wherein before plate-frame filtering, be first added in the zymotic fluid containing S- abscisic acids solid NaOH adjust pH value to
It is filtered after 5.5.
Then the above-mentioned zymotic fluid for having adjusted pH value passes through plate-frame filtering, and control filter pressure is 0.5MPa, filter efficiency
For 5L/min, filtrate is obtained.
Above-mentioned filtrate is subjected to membrane filtration, wherein membrane filtration includes the micro-filtration set gradually, ultrafiltration and nanofiltration；
In microfiltration process, the suspended particulate in filtrate, strain and other large granular impurities after plate-frame filtering are filtered
It removes；The pressure of micro-filtration is 0.6MPa, and the aperture of filter efficiency 4L/min, microfiltration membranes are 50nm；
In ultra-filtration process, the macro-molecular protein in filtrate, glucose after micro-filtration etc. are filtered out；The pressure of ultrafiltration is
The aperture of 0.7MPa, filter efficiency 3L/min, ultrafiltration membrane are 20000D；
In nanofiltration process, the filtrate after ultrafiltration is further filtered, filters out part small molecule, obtains more pure S-
Abscisic acid solution；Wherein, nanofiltration is divided into two-stage, respectively level-one nanofiltration and two level nanofiltration；The pressure of level-one nanofiltration is 2MPa, mistake
Filter efficiency is 3.2L/min, and the aperture of level-one nanofiltration and two level nanofiltration is 100D；
Filtrate after membrane filtration is further detached by extraction, and extractant selects ethyl acetate, and controls extraction
It is 3.0 to take the pH value of system, realizes the separation of S- abscisic acids and oils, Ester, obtains the extract liquor containing S- abscisic acids.
(3) extract liquor obtained above containing S- abscisic acids can be obtained into weight by condensing crystallizing, recrystallization, drying
Measure the S- abscisic acid finished products that percentage is 95% or more.
Wherein, the temperature of condensing crystallizing is 40 DEG C, and the time of condensing crystallizing is 45min；
The temperature of recrystallization is 40 DEG C, and the time of condensing crystallizing is 50min；
Dry temperature is 40 DEG C, and the dry time is 4.5 hours.
It, using different component fermentation medium, is adopted using experiment of single factor method in the case where other conditions are consistent
The preparation that S- abscisic acids are carried out with above-mentioned preparation method, the results are shown in Table 1.
Influence result of 1 fermentation medium of table to fermentation period and S- abscisic acid yield
Embodiment 2
Influence of the fermentation medium pH value regulative mode to fermentation period and S- abscisic acid yield
Using experiment of single factor method, the preparation of S- abscisic acids, fermentation training are carried out according to the preparation method that embodiment 1 provides
The culture medium for testing C in the table 1 of base selection embodiment 1 is supported, in the case where other conditions are consistent, only changes pH regulative modes
(experiment 2~experiment 4 in corresponding table 2), using different fermentations Medium's PH Value regulative mode, the results are shown in Table 2.
Influence of the 2 fermentation medium pH value regulative mode of table to fermentation period and S- abscisic acid yield
Embodiment 3
Influence of the fermentation medium pH value to fermentation period and S- abscisic acid yield
Using experiment of single factor method, the preparation of S- abscisic acids, fermentation training are carried out according to the preparation method that embodiment 1 provides
The culture medium for testing C in the table 1 of base selection embodiment 1 is supported, in the case where other conditions are consistent, only changes fermentation medium
PH value the results are shown in Table 3 using different fermentations Medium's PH Value.
Influence of the 3 fermentation medium pH value of table to fermentation period and S- abscisic acid yield
Embodiment 4
Influence of the zymotic fluid pH value to plate-frame filtering effect
Using experiment of single factor method, the preparation of S- abscisic acids, fermentation training are carried out according to the preparation method that embodiment 1 provides
The culture medium for testing C in the table 1 of base selection embodiment 1 is supported, in the case where other conditions are consistent, before plate-frame filtering, is changed
Become zymotic fluid pH value and the results are shown in Table 4 using different zymotic fluid pH value.
Influence of the 4 zymotic fluid pH value of table to plate-frame filtering effect
Remarks：This experiment zymotic fluid weight is 50L, content 8000ppm.
Embodiment 5
Influence of the plate-frame filtering parameter to plate-frame filtering effect
Using experiment of single factor method, the preparation of S- abscisic acids, fermentation training are carried out according to the preparation method that embodiment 1 provides
The culture medium for testing C in the table 1 of base selection embodiment 1 is supported, in the case where other conditions are consistent, changes plate-frame filtering ginseng
Number, is filtered using different plate-frame filtering parameters, the results are shown in Table 5.
Influence of the 5 plate-frame filtering parameter of table to plate-frame filtering effect
Remarks：This experiment zymotic fluid weight is 50L, content 8000ppm.
Embodiment 6
Influence of the membrane filtration parameter to membrane filtration effect
Using experiment of single factor method, the preparation of S- abscisic acids, fermentation training are carried out according to the preparation method that embodiment 1 provides
The culture medium for testing C in the table 1 of base selection embodiment 1 is supported, in the case where other conditions are consistent, changes membrane filtration parameter,
It is filtered using different membrane filtration parameters, the results are shown in Table 6.
Influence of the 6 membrane filtration parameter of table to membrane filtration effect
Remarks：This experiment sheet frame filtrate used, micro-filtration clear liquid, ultrafiltration clear liquid, level-one nanofiltration raffinate weight are respectively
100L、90L、80L、70L。
Embodiment 7
Influence of the extractant component to effect of extracting
Using experiment of single factor method, the preparation of S- abscisic acids, fermentation training are carried out according to the preparation method that embodiment 1 provides
The culture medium for testing C in the table 1 of base selection embodiment 1 is supported, in the case where other conditions are consistent, changes extractant type,
Filtrate is extracted using different extractants, the results are shown in Table 7.
Influence of the 7 extractant component of table to effect of extracting
Remarks：This experiment concentration liquid hold-up used is 20000ppm.
Embodiment 8
Influence of the extraction system pH value to effect of extracting
Using experiment of single factor method, the preparation of S- abscisic acids, fermentation training are carried out according to the preparation method that embodiment 1 provides
The culture medium for testing C in the table 1 of base selection embodiment 1 is supported, in the case where other conditions are consistent, changes extraction system pH
Value, extracts filtrate using different extraction system pH value, the results are shown in Table 8.
Influence of the 8 extraction system pH value of table to effect of extracting
Remarks：This experiment concentration liquid hold-up used is 20000ppm.
Embodiment 9
The influence of condensing crystallizing, recrystallization, drying temperature to S- abscisic acid purity
Using experiment of single factor method, the preparation of S- abscisic acids, fermentation training are carried out according to the preparation method that embodiment 1 provides
The culture medium that C is tested in the table 1 of base selection embodiment 1 is supported, in the case where other conditions are consistent, using different concentration knots
Brilliant, recrystallization, drying temperature, the results are shown in Table 9.
9 condensing crystallizing of table, recrystallization, drying temperature are to the influence result of S- abscisic acid purity
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (11)
1. a kind of preparation method of S- abscisic acids, it is characterised in that：The preparation method comprises the following steps：
(1) it is obtained containing S- abscisic acids by seed culture, fermented and cultured using the strain for generating S- abscisic acids that can ferment
Zymotic fluid；
(2) by the zymotic fluid containing S- abscisic acids described in step (1) through plate-frame filtering, membrane filtration, acid out, extraction is obtained by extraction
Liquid；
(3) by step (2) described extract liquor by condensing crystallizing, recrystallize, be dried to obtain purity be 95% or more S- lure it is anti-
Plain finished product.
2. a kind of preparation method of S- abscisic acids according to claim 1, it is characterised in that：Step is fermented described in (1)
Culture is specially：It maintains the pH value of fermentation medium in 4-7, carries out fermented and cultured.
3. a kind of preparation method of S- abscisic acids according to claim 2, it is characterised in that：The maintenance fermentation medium
PH value be specially in 4-7：Continuous supplementation liquid alkali when the pH value of fermentation medium is less than 4；Continuous supplementation is sent out when pH value is higher than 7
Ferment culture medium feed liquid.
4. a kind of preparation method of S- abscisic acids according to claim 1, it is characterised in that：Step (2) the sheet frame mistake
Before filter, solid NaOH is added into the zymotic fluid containing S- abscisic acids and adjusts pH value to 4-7, in the plate-frame filtering, control
Pressing pressure 0.05-0.5MPa.
5. a kind of preparation method of S- abscisic acids according to claim 1, it is characterised in that：Step (2) described membrane filtration
Including micro-filtration, ultrafiltration and nanofiltration process.
6. a kind of preparation method of S- abscisic acids according to claim 5, it is characterised in that：Step (2) the micro-filtration work
Sequence is specially：It is 0.05-0.6MPa in pressure, filtration temperature carries out micro-filtration under conditions of being not more than 60 DEG C.
7. a kind of preparation method of S- abscisic acids according to claim 5, it is characterised in that：Step (2) the ultrafiltration work
Sequence is specially：It is 0.05-1.0MPa in pressure, filtration temperature carries out ultrafiltration under conditions of being not more than 60 DEG C.
8. a kind of preparation method of S- abscisic acids according to claim 5, it is characterised in that：Step (2) the nanofiltration work
Sequence includes level-one nanofiltration and two level nanofiltration, and the level-one nanofiltration is specially：It is 0.1-2.0MPa in pressure, filtration temperature is not more than
Level-one nanofiltration is carried out under conditions of 60 DEG C；The two level nanofiltration is specially：It is 0.5-10.0MPa in pressure, filtration temperature is little
Two level nanofiltration is carried out under conditions of 60 DEG C.
9. a kind of preparation method of S- abscisic acids according to claim 1, it is characterised in that：In step (2) described extraction,
The extractant used is at least one selected from petroleum ether, ethyl acetate, butyl acetate, n-amyl alcohol, ethyl alcohol, acetone, methanol, extraction
The pH value of system is 1-4.
10. a kind of preparation method of S- abscisic acids according to claim 1, it is characterised in that：Step (3) the concentration knot
Brilliant, recrystallization, dry temperature are 40-80 DEG C.
11. a kind of preparation method of S- abscisic acids according to claim 1, it is characterised in that：Step (1) the fermentation training
Foster nutrient media components include soybean protein, lactose, glucose, maltose, maltose, sucrose, maltodextrin, starch, corn egg
In vain, cottonseed meal, potassium dihydrogen phosphate, epsom salt, manganese sulfate monohydrate, ferrous sulfate heptahydrate, white vitriol, ammonium sulfate,
At least one of salad oil.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112690290A (en) * | 2020-12-30 | 2021-04-23 | 阳田生物科技(江苏)有限公司 | Preparation method of bacillus subtilis plant growth promoter |
| CN113981016A (en) * | 2021-12-17 | 2022-01-28 | 四川龙蟒福生科技有限责任公司 | Fermentation formula for reducing S-ABA impurities in fermentation production |
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| CN107058410A (en) * | 2017-04-18 | 2017-08-18 | 四川龙蟒福生科技有限责任公司 | A kind of preparation method of S abscisic acids |
| CN110423155A (en) * | 2019-07-31 | 2019-11-08 | 广州新农科肥业科技有限公司 | A kind of Fertilizer nutrient formula that the effective medicinal ingredient of the root of purple-flowered peucedanum is promoted |
| CN112674097A (en) * | 2021-01-23 | 2021-04-20 | 青岛海大汇信生物科技有限公司 | Chitosan oligosaccharide and S-abscisic acid compound sterilization regulator and preparation method thereof |
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| CN107058410A (en) * | 2017-04-18 | 2017-08-18 | 四川龙蟒福生科技有限责任公司 | A kind of preparation method of S abscisic acids |
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| RU2085588C1 (en) * | 1992-09-16 | 1997-07-27 | Товарищество с ограниченной ответственностью "Биотэк" | Method of isolation of abscisic acid from cultural fluid |
| CN104988188A (en) * | 2015-07-20 | 2015-10-21 | 中国药科大学 | Method for increasing fermentation output of abscisic acid |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112690290A (en) * | 2020-12-30 | 2021-04-23 | 阳田生物科技(江苏)有限公司 | Preparation method of bacillus subtilis plant growth promoter |
| CN113981016A (en) * | 2021-12-17 | 2022-01-28 | 四川龙蟒福生科技有限责任公司 | Fermentation formula for reducing S-ABA impurities in fermentation production |
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| CN107058410A (en) | 2017-08-18 |
| WO2018192529A1 (en) | 2018-10-25 |
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