CN108524566A - A kind of production method of environmentally protective low pesticide residue ginseng stem and leave general saponin - Google Patents
A kind of production method of environmentally protective low pesticide residue ginseng stem and leave general saponin Download PDFInfo
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- CN108524566A CN108524566A CN201810415236.XA CN201810415236A CN108524566A CN 108524566 A CN108524566 A CN 108524566A CN 201810415236 A CN201810415236 A CN 201810415236A CN 108524566 A CN108524566 A CN 108524566A
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- Prior art keywords
- pesticide residue
- low
- ginseng stem
- production method
- environmentally protective
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
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- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of extracted from gen-seng haulms low pesticide residue total saposins environmentally protective new method.The present invention handles the pesticide residue in gen-seng haulms raw material using aqueous alcoholic solvent first, and then the ginseng stem and leave general saponin of low pesticide residue is produced using the gen-seng haulms raw material after removal pesticide residue.The production method of the present invention is environmentally protective, and at low cost relative to existing production method, solvent usage amount substantially reduces, and reduces environmental protection pressure caused by solvent.Preparation method through the invention is by facts have proved that pesticide residue is low, and relatively existing using based on solvent extraction and resin adsorption, to remove, the method for pesticide residue is more practical, convenient and effect is good.
Description
Technical field
The present invention relates to a kind of production methods of the ginseng stem and leave general saponin of environmentally protective low pesticide residue, and the method can
Environment-friendly high-efficiency easily produces ginseng stem and leave general saponin using gen-seng haulms.
Background technology
Ginseng stem and leave general saponin is widely used in treatment and healthcare field, has various pharmacological actions.To nerveous system
System has adjustment effect, and central inhibitory action may be related with the inhibition of ATP enzyme.Ginsenoside is made with male sex hormone sample
With adjustable endocrine.There is anti-hypoxemia to act on, it may be related with other corticocerebral Hypoxia tolerances of raising.To angiocarpy
Its cardiac effect of system is related with intracellular ATP is increased.By long term, can gradually from the blocking tumour cell G1 phases to
The S phases, which are transitioned into, blocks the S phases to G2 phase transition.Changeable tumour cell rna content, causes S-180 sarcoma cell characteristics to change
Become, to inhibit the growth of tumour cell, P- in induction hepatomicrosome may be by the protective effect of Experimental Hepatic Damage
The increase of 450 contents, adjusts liver metabolism, the anti-inflammatory equal influence to a variety of biochemical processes, to specific inflammatory have confrontation and
Inhibiting effect.Current ginsenoside production method is mainly extracted with water or moderate ethyl alcohol, and after through macroporous resin column
It refines and obtains.The processing of pesticide residue is with CO 2 supercritical, based on solvent extraction and resin adsorption.Above method is because a large amount of
Using water and macroreticular resin, the use of solvent and cause certain environmental protection pressure.
Invention content
For above-mentioned background technology there are the problem of, the present invention is intended to provide a kind of environmentally protective production technology production is low
The method of pesticide residue ginseng stem and leave general saponin.
For this purpose, the present invention uses following technical scheme：A kind of environmentally protective low pesticide residue ginseng stem and leave general saponin
Production method, characterized in that include the following steps：
Step 1：Gen-seng haulms raw material is carried out using the low-carbon alcohol solution of water content 0~20% first to take off residual processing；
Step 2：Ginseng stem and leave general saponin is extracted with 0~40% ethyl alcohol of water content, it is then concentrated, be dried to obtain low agriculture
The remaining ginseng stem and leave general saponin of medicine.
As supplement to above-mentioned technical proposal and perfect, the invention also includes following technical characteristics.
The de- residual processing in step 1 is specially the low-carbon alcohol solution with water content 0~20% to gen-seng haulms original
Material carries out de- residual processing 2 times, and first time extract liquor is 5 times of raw material weight, is extracted 1 hour, second of extract liquor is raw material weight
3 times of amount extract 0.5 hour.
The low-carbon alcohols in step 1 are methanol, ethyl alcohol, normal propyl alcohol, low carbon mixed alcohol and straight chain and attachment low-carbon alcohols
In one or more mixing.
The present invention can reach following advantageous effect：1, production method of the invention is environmentally protective, relative to existing life
Production method is at low cost, and solvent usage amount substantially reduces, and reduces environmental protection pressure caused by solvent.2, preparation side through the invention
Method relatively existing removes pesticide residue by facts have proved that pesticide residue is low using based on solvent extraction and resin adsorption
Method is more practical, convenient and effect is good.
Specific implementation mode
Embodiment 1
The gen-seng haulms raw material that 100 grams of crushing are put into 5000mL reaction vessels, is then added absolute ethyl alcohol 500g, adds
Heat reflux 1 hour, filtering removal ethanol solution；300g absolute ethyl alcohols are then added and carry out second of extraction, it is small to be heated to reflux 0.5
When, filtering removal ethanol solution.20% ethanol solution 2000ml is then added, is heated to reflux 2 hours, collects and extracts for the first time
Liquid；20% ethanol solution 1000ml is then added, is heated to reflux 1 hour, collects second of extracting solution；Merge extracting solution twice,
Concentrated extracting solution, dry ginseng stem and leave general saponin：4.2 grams, content：41.2%, pesticide residue DDT：20ppb, six or six
Six：15ppb, pentachloronitrobenzene：12ppb.
Embodiment 2
Normal propyl alcohol 500g, heating is then added in the gen-seng haulms raw material that 100 grams of crushing are put into 5000mL reaction vessels
Reflux 1 hour, filtering removal normal propyl alcohol solution；300g normal propyl alcohols are then added and carry out second of extraction, it is small to be heated to reflux 0.5
When, filtering removal normal propyl alcohol solution.2000 grams of 35% ethanol solution is then added, is heated to reflux 2 hours, collects and extracts for the first time
Liquid；1000 grams of 35% ethanol solution is then added, is heated to reflux 1 hour, collects second of extracting solution；Merge extracting solution twice,
Concentrated extracting solution, dry 3.7 grams of low pesticide residue ginseng stem and leave general saponin, content 48.7%, pesticide residue DDT：
20ppb, six six six：15ppb, pentachloronitrobenzene：12ppb.
Embodiment 3
The gen-seng haulms raw material that 100 grams of crushing are put into 5000mL reaction vessels, is then added 85% second of volume fraction
Alcohol 500g is heated to reflux 1 hour, filtering removal ethanol solution；85% ethyl alcohol of 300g volume fractions is then added to carry out second
Extraction, is heated to reflux 0.5 hour, filtering removal ethanol solution.40% ethanol solution 2000ml is then added, it is small to be heated to reflux 2
When, collect first time extracting solution；40% ethanol solution 1000ml is then added, is heated to reflux 1 hour, collects second and extracts
Liquid；Merge extracting solution twice, concentrated extracting solution, dry ginseng stem and leave general saponin：4.5 grams, content：40.9%, pesticide residue
DDT：18ppb, six six six：14ppb, pentachloronitrobenzene：9ppb.
The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent thereof.
Claims (3)
1. a kind of production method of environmentally protective low pesticide residue ginseng stem and leave general saponin, it is characterised in that including following step
Suddenly：
Step 1：Gen-seng haulms raw material is carried out using the low-carbon alcohol solution of water content 0~20% first to take off residual processing；
Step 2：Ginseng stem and leave general saponin is extracted with 0~40% ethyl alcohol of water content, it is then concentrated, to be dried to obtain low pesticide residual
The ginseng stem and leave general saponin stayed.
2. a kind of production method of environmentally protective low pesticide residue ginseng stem and leave general saponin according to claim 1,
It is characterized in that：The de- residual processing in step 1 is specially the low-carbon alcohol solution with water content 0~20% to gen-seng haulms
Raw material carries out de- residual processing 2 times, and first time extract liquor is 5 times of raw material weight, is extracted 1 hour, second of extract liquor is raw material
3 times of weight extract 0.5 hour.
3. a kind of production method of environmentally protective low pesticide residue ginseng stem and leave general saponin according to claim 1 or 2,
It is characterized in that：The low-carbon alcohols in step 1 are that methanol, ethyl alcohol, normal propyl alcohol, low carbon mixed alcohol and straight chain and attachment are low
One or more mixing in carbon alcohol.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810415236.XA CN108524566A (en) | 2018-05-03 | 2018-05-03 | A kind of production method of environmentally protective low pesticide residue ginseng stem and leave general saponin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810415236.XA CN108524566A (en) | 2018-05-03 | 2018-05-03 | A kind of production method of environmentally protective low pesticide residue ginseng stem and leave general saponin |
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| CN108524566A true CN108524566A (en) | 2018-09-14 |
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| CN201810415236.XA Pending CN108524566A (en) | 2018-05-03 | 2018-05-03 | A kind of production method of environmentally protective low pesticide residue ginseng stem and leave general saponin |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111840345A (en) * | 2020-08-25 | 2020-10-30 | 江西纳弗堂制药有限公司 | Extraction process of total saponins of ginseng stem and leaf |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101810658A (en) * | 2010-05-07 | 2010-08-25 | 青岛东仁生物医药技术有限公司 | Method for removing residual pesticide from ginseng |
| CN103550273A (en) * | 2013-11-04 | 2014-02-05 | 湖南绿蔓生物科技股份有限公司 | Method for extracting low-pesticide-residue ginsenoside from ginsengs |
| CN104173399A (en) * | 2014-09-15 | 2014-12-03 | 刘智谋 | Preparation method of ginseng stem and leaf total saponins with low pesticide residues |
| CN104490966A (en) * | 2014-12-04 | 2015-04-08 | 宁波中药制药有限公司 | Method for removing pesticide residues in ginseng stem leaf extracts |
| CN105640970A (en) * | 2016-01-11 | 2016-06-08 | 成都普瑞法科技开发有限公司 | Effective part of ginseng stems and leaves, as well as preparation method and application thereof |
-
2018
- 2018-05-03 CN CN201810415236.XA patent/CN108524566A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101810658A (en) * | 2010-05-07 | 2010-08-25 | 青岛东仁生物医药技术有限公司 | Method for removing residual pesticide from ginseng |
| CN103550273A (en) * | 2013-11-04 | 2014-02-05 | 湖南绿蔓生物科技股份有限公司 | Method for extracting low-pesticide-residue ginsenoside from ginsengs |
| CN104173399A (en) * | 2014-09-15 | 2014-12-03 | 刘智谋 | Preparation method of ginseng stem and leaf total saponins with low pesticide residues |
| CN104490966A (en) * | 2014-12-04 | 2015-04-08 | 宁波中药制药有限公司 | Method for removing pesticide residues in ginseng stem leaf extracts |
| CN105640970A (en) * | 2016-01-11 | 2016-06-08 | 成都普瑞法科技开发有限公司 | Effective part of ginseng stems and leaves, as well as preparation method and application thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111840345A (en) * | 2020-08-25 | 2020-10-30 | 江西纳弗堂制药有限公司 | Extraction process of total saponins of ginseng stem and leaf |
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