CN108523819B - Fluorescent navigation endoscope system with photometric feedback and automatic laser power adjustment method - Google Patents
Fluorescent navigation endoscope system with photometric feedback and automatic laser power adjustment method Download PDFInfo
- Publication number
- CN108523819B CN108523819B CN201810227406.1A CN201810227406A CN108523819B CN 108523819 B CN108523819 B CN 108523819B CN 201810227406 A CN201810227406 A CN 201810227406A CN 108523819 B CN108523819 B CN 108523819B
- Authority
- CN
- China
- Prior art keywords
- laser
- endoscope
- light
- output
- power
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000000034 method Methods 0.000 title claims abstract description 29
- 238000003384 imaging method Methods 0.000 claims abstract description 15
- 238000012632 fluorescent imaging Methods 0.000 claims abstract description 8
- 230000003287 optical effect Effects 0.000 claims description 16
- 238000005286 illumination Methods 0.000 claims description 7
- 230000005855 radiation Effects 0.000 claims description 7
- 230000005284 excitation Effects 0.000 claims description 6
- 238000005259 measurement Methods 0.000 claims description 2
- 239000000463 material Substances 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000000258 photobiological effect Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000002222 downregulating effect Effects 0.000 description 1
- 238000000799 fluorescence microscopy Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000001215 fluorescent labelling Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
- A61B1/00163—Optical arrangements
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0071—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by measuring fluorescence emission
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0082—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes
- A61B5/0084—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes for introduction into the body, e.g. by catheters
Abstract
The invention discloses a fluorescent navigation endoscope system based on photometric feedback and a laser power automatic adjustment method, wherein guide light is added into a light source, a spectroscope is added onto an imaging light path, the guide light intensity entering the imaging light path is detected through a photoelectric detector, the distance is obtained through operation and is fed back to the system, the output light power can be adjusted in real time, fluorescent imaging is met, meanwhile, the tissue can be prevented from being irradiated by high-power density laser for a long time, and the damage of the laser to biological tissue is reduced.
Description
Technical Field
The invention relates to a fluorescence navigation endoscope system, in particular to a fluorescence navigation endoscope system based on photometric feedback and an automatic laser power adjusting method.
Background
Nowadays, more and more endoscope systems have a fluorescence labeling function, in order to achieve higher fluorescence excitation efficiency and obtain better imaging effects, a laser is generally used as a fluorescence excitation light source, and in order to ensure the overall imaging effect of the system, the excitation light power emitted from the front section of the endoscope is generally larger. However, when the output laser power set by most fluorescence endoscope systems is a fixed value and the tissue needs to be observed in close proximity, the illumination area becomes smaller, so that the received light power (i.e., the illuminance) per unit area is increased, which causes burns or other photo-biological safety problems.
Accordingly, the prior art is still in need of improvement and development.
Disclosure of Invention
The invention aims to provide a fluorescence navigation endoscope system based on photometric feedback and a laser power automatic adjustment method, and aims to solve the problems that the output laser power of the existing endoscope imaging system is a constant value, and burns or other photo-biological safety are easy to cause when the endoscope imaging system is close to tissue for observation.
The technical scheme of the invention is as follows: a fluorescence navigation endoscope laser power automatic adjustment method based on photometric feedback specifically comprises the following steps:
step S1: the laser emitted by the laser and the guiding light emitted by the guiding light source are transmitted through the same guiding light beam and coupled into the endoscope;
step S2: the laser and the guiding light are emitted from the front end of the endoscope and reach the observed tissue, and the exciting light, the fluorescent light and the guiding light reflected by the observed tissue are collected by the endoscope;
step S3: the excitation light is filtered by the filter, and the fluorescence and the guiding light are focused by the lens, wherein the fluorescence is imaged on the camera through the dichroic spectroscope, the guiding light is reflected by the dichroic spectroscope and is incident on the photoelectric detector;
step S4: the photoelectric detector converts the guiding light signal into output voltage and outputs the output voltage to the light source control module;
step S5: the light source control module fits a relation curve between the actual output light power of the laser and the output voltage of the photoelectric detector;
step S6: the light source control module automatically obtains the light power to be output of the laser according to the relation curve of the actual output light power of the laser and the output voltage of the photoelectric detector, and then controls the actual light power output of the laser.
In the method for automatically adjusting the laser power of the fluorescence navigation endoscope based on photometric feedback, specifically, in the step S5, distances between the front end surfaces of different endoscopes and observed tissues are tested, and the photoelectric detectors correspondingly output different output voltages to obtain the relation between the output voltages of the photoelectric detectors and the distances between the front end surfaces of the endoscopes and the observed tissues; the laser radiation illumination required by the fluorescent imaging of the system isUnder the distances between the front end surfaces of different endoscopes and the observed tissues, the irradiation illuminance E is actually measured, and the output light power of the laser emitted from the front end of the endoscope is adjusted so that the actual measurement value E is equal to +.>Obtaining the relation between the output light power and the distance of the laser emitted from the front end of the endoscope; finally, the relation between the output voltage of the photoelectric detector and the output light power of the laser emitted from the front end of the endoscope is obtained, and the light source control module fits a P-V curve according to the relation between the output voltage of the photoelectric detector and the output light power P of the laser emitted from the front end of the endoscope, namely, the relation curve between the actual output light power of the laser and the output voltage of the photoelectric detector.
According to the automatic adjustment method for laser power of the fluorescent navigation endoscope based on photometric feedback, the furthest observation distance is set to be Ncm according to the application of the endoscope, and the output voltage of the photoelectric detector is correspondingly set to be V N At this time, the output light power of the laser light emitted from the distal end of the endoscope becomes the maximum value P N ：
For D < N, at this time, according to the P-V curve, the output light power P of the laser emitted from the front end of the endoscope can be obtained;
for D>When N is the same, the output light power of the laser emitted from the front end of the endoscope is uniform to P N ；
Wherein D is the distance between the front end surface of the actual endoscope and the observed tissue, N is the furthest observation distance of the endoscope, and V N The output voltage corresponding to the photoelectric detector when the furthest observation distance of the endoscope is Ncm, P N The output light power of the laser beam emitted from the distal end of the endoscope when the farthest observation distance of the endoscope is Ncm is P, which is the output light power of the laser beam emitted from the distal end of the endoscope actually required.
According to the fluorescence navigation endoscope laser power automatic adjustment method based on photometric feedback, the adjustment of the actual optical power of the laser is preferably realized by adopting a pulse width modulation method, wherein the adopted pulse modulation frequency is preferably 10kHz.
The automatic adjustment method of the laser power of the fluorescence navigation endoscope based on photometric feedback, wherein in D<In this case, the pulse width modulation duty ratio of the output light power of the laser beam emitted from the distal end of the endoscope is P/>*100, wherein P is the output light power of the laser emitted from the front end of the endoscope which is actually needed, < +.>The output light power of the laser beam emitted from the distal end of the endoscope when the farthest observation distance of the endoscope is Ncm.
According to the fluorescence navigation endoscope laser power automatic adjustment method based on the photometric feedback, the adjustment of the actual optical power of the laser can be realized in various modes such as amplitude modulation.
A fluorescence navigation endoscope system based on photometric feedback, which adopts the automatic adjustment method of laser power of the fluorescence navigation endoscope based on photometric feedback according to any one of the above, wherein the fluorescence navigation endoscope system based on photometric feedback comprises a laser, a guiding light source, a light guide beam, an endoscope, a filter, a lens, a dichroic spectroscope, a camera, a photoelectric detector and a light source control module; the photosensitive surface of the photoelectric detector is positioned on the imaging surface of the guiding light;
the laser emitted by the laser and the guiding light emitted by the guiding light source are transmitted through the same guiding light beam and coupled into the endoscope; the laser and the guiding light are emitted from the front end of the endoscope and reach the observed tissue, the exciting light, the fluorescent light and the guiding light reflected by the observed tissue are collected by the endoscope, wherein the exciting light is filtered by a filter, the fluorescent light and the guiding light are focused by a lens, the fluorescent light is imaged on a camera through a dichroic spectroscope, the guiding light is reflected by the dichroic spectroscope and is incident on a photoelectric detector; the photoelectric detector converts the guiding light signal into output voltage and outputs the output voltage to the light source control module;
the light source control module fits a P-V curve according to the relation between the output voltage of the photoelectric detector and the output light power of laser emitted from the front end of the endoscope; according to the P-V curve, the light source controller automatically obtains the light power to be output by the laser, and then controls the actual light power output of the laser.
In the fluorescence navigation endoscope system based on photometric feedback, the photoelectric detector preferably adopts a photoelectric avalanche diode.
The invention has the beneficial effects that: the invention provides a fluorescent navigation endoscope system based on photometric feedback and an automatic laser power adjusting method, wherein guide light is added into a light source, a spectroscope is added onto an imaging light path, the guide light intensity entering the imaging light path is detected through a photoelectric detector, the distance is obtained through calculation and is fed back to the system, the output light power can be adjusted in real time, fluorescent imaging is met, meanwhile, the tissue can be prevented from being irradiated by high-power density laser for a long time, and the damage of the laser to biological tissue is reduced.
Drawings
FIG. 1 is a schematic diagram of a fluorescence navigation endoscope system based on photometric feedback in the present invention.
FIG. 2 is a flow chart of the steps of the automatic laser power adjustment method of the fluorescence navigation endoscope based on photometric feedback in the present invention.
Fig. 3 is a schematic diagram of the adjustment of output optical power by pwm in the present invention.
Fig. 4 is a schematic diagram of the adjustment of output optical power by amplitude modulation in the present invention.
Detailed Description
Embodiments of the present invention are described in detail below, examples of which are illustrated in the accompanying drawings, wherein the same or similar reference numerals refer to the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the drawings are exemplary only for explaining the present invention and are not to be construed as limiting the present invention.
In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more of the described features. In the description of the present invention, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.
In the description of the present invention, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically connected, electrically connected or can be communicated with each other; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art according to the specific circumstances.
In the present invention, unless expressly stated or limited otherwise, a first feature "above" or "below" a second feature may include both the first and second features being in direct contact, as well as the first and second features not being in direct contact but being in contact with each other through additional features therebetween. Moreover, a first feature being "above," "over" and "on" a second feature includes the first feature being directly above and obliquely above the second feature, or simply indicating that the first feature is higher in level than the second feature. The first feature being "under", "below" and "beneath" the second feature includes the first feature being directly under and obliquely below the second feature, or simply means that the first feature is less level than the second feature.
The following disclosure provides many different embodiments, or examples, for implementing different features of the invention. In order to simplify the present disclosure, components and arrangements of specific examples are described below. They are, of course, merely examples and are not intended to limit the invention. Furthermore, the present invention may repeat reference numerals and/or letters in the various examples, which are for the purpose of brevity and clarity, and which do not themselves indicate the relationship between the various embodiments and/or arrangements discussed. In addition, the present invention provides examples of various specific processes and materials, but one of ordinary skill in the art will recognize the application of other processes and/or the use of other materials.
As shown in fig. 1, a fluorescence navigation endoscope system based on photometric feedback comprises a laser 1, a guiding light source 2, a light guide beam 3, an endoscope 4, a filter 5, a lens 6, a dichroic spectroscope 7, a camera 8, a photoelectric detector 9 and a light source control module 10; wherein the photosensitive surface of the photodetector 9 is positioned on the imaging surface of the guiding light;
the laser light emitted by the laser 1 and the guiding light emitted by the guiding light source 2 are transmitted through the same guiding light beam 3 and coupled into the endoscope 4; the laser light and the guiding light are emitted from the front end of the endoscope 4 and reach the observed tissue, the exciting light, the fluorescent light (the exciting light and the fluorescent light are formed after the laser light is reflected by the observed tissue) and the guiding light reflected by the observed tissue are collected by the endoscope 4, wherein the exciting light is filtered by a filter 5, the fluorescent light and the guiding light are focused by a lens 6, wherein the fluorescent light is imaged on a camera 8 through a dichroic spectroscope 7, the guiding light is reflected by the dichroic spectroscope 7 and is incident on a photoelectric detector 9; the photodetector 9 converts the guiding light signal into an output voltage and outputs the output voltage to the light source control module 10;
testing different distances D (D is the distance between the front end face of the endoscope 4 and the observed tissue), and correspondingly outputting different output voltages V by the photoelectric detector 9 to obtain the output voltage V and the distance of the photoelectric detector 9A relation of D; the laser radiation illumination required by the fluorescent imaging of the system isAt different distances D, the illuminance E is actually measured by adjusting the output power P of the laser light emitted from the distal end of the endoscope 4 (the output power P of the laser light emitted from the distal end of the endoscope 4 and the actual output power of the laser 1Equal) such that the measured value E is equal to +.>The relation between the output light power P of the laser light emitted from the front end of the endoscope 4 and the distance D can be obtained; finally, the relation between the output voltage V of the photodetector 9 and the output light power P of the laser light emitted from the front end of the endoscope 4 (namely, the output voltage V of the photodetector 9 and the actual output light power of the laser 1 are obtained>The light source control module 10 fits a P-V curve according to the relation between the output voltage V of the photoelectric detector 9 and the output light power P of laser emitted from the front end of the endoscope 4; in this way, the output voltage V of the photodetector 9 plays a role in distance guidance in the system, the output voltage V of the photodetector 9 is input to the light source controller 10, and the light source controller 10 automatically obtains the light power to be output of the laser 1 through the P-V curve>The actual optical power output of the laser 1 is then controlled.
In particular, the photodetector 9 may be implemented using a variety of light source detection structures. In this embodiment, the photodetector 9 preferably employs a photodiode.
As shown in fig. 2, the method for automatically adjusting the laser power of the fluorescence navigation endoscope system based on photometric feedback specifically includes the following steps:
step S1: the laser light emitted by the laser 1 and the guiding light emitted by the guiding light source 2 are transmitted through the same guiding light beam 3 and coupled into the endoscope 4;
step S2: the laser light and the guiding light are emitted from the front end of the endoscope 4 and reach the observed tissue, and the exciting light, the fluorescence (the exciting light and the fluorescence are formed after the laser light is reflected by the observed tissue) and the guiding light reflected by the observed tissue are collected by the endoscope 4;
step S3: the excitation light is filtered by the filter 5, and the fluorescence and the guiding light are focused by the lens 6, wherein the fluorescence is imaged on the camera 8 through the dichroic spectroscope 7, the guiding light is reflected by the dichroic spectroscope 7 and is incident on the photodetector 9;
step S4: the photodetector 9 converts the guiding light signal into an output voltage and outputs the output voltage to the light source control module 10;
step S5: the light source control module 10 fits the actual output light power of the laser 1A relation to the output voltage V of the photodetector 9;
step S6: the light source control module 10 outputs the light power according to the actual output of the laser 1The dependence on the output voltage V of the photodetector 9 automatically yields the desired output light power of the laser 1 +.>The actual optical power output of the laser 1 is then controlled.
Specifically, in the step S5, different distances D (D is the distance between the front end surface of the endoscope 4 and the observed tissue) are tested, and the photo detector 9 correspondingly outputs different output voltages V, so as to obtain the relationship between the output voltage V of the photo detector 9 and the distance D; the laser radiation illumination required by the fluorescent imaging of the system isAt different distances D, the illuminance E is actually measured by adjusting the output light power P of the laser light emitted from the distal end of the endoscope 4 (the output light power P of the laser light emitted from the distal end of the endoscope 4 and the actual output light power of the laser 1->Equal) such that the measured value E is equal to +.>The relation between the output light power P of the laser light emitted from the front end of the endoscope 4 and the distance D can be obtained; finally, the relation between the output voltage V of the photodetector 9 and the output light power P of the laser light emitted from the front end of the endoscope 4 (namely, the output voltage V of the photodetector 9 and the actual output light power of the laser 1The light source control module 10 fits a P-V curve according to the relationship between the output voltage V of the photodetector 9 and the output light power P of the laser light emitted from the distal end of the endoscope 4.
Further, the closer the distance between the distal end face of the endoscope 4 and the tissue to be observed is, the smaller the irradiation area is, the larger the illuminance of the guide light radiation is, and the stronger the signal entering the photodetector 9 is. Therefore, by testing the different distances D, the photodetectors 9 correspondingly output different output voltages V, and the relationship between the output voltages V of the photodetectors 9 and the distances D can be obtained as shown in table 1 below.
TABLE 1 relation of output voltage V of photodetector 9 to distance D
The laser radiation illumination required by the fluorescent imaging of the system isAt different distances D, the illuminance E of the radiation is actually measured by adjustingThe output power P of the laser light emitted from the distal end of the endoscope 4 (the output power P of the laser light emitted from the distal end of the endoscope 4 and the actual output power of the laser 1 +.>Equal) such that the measured value E is equal to +.>The relationship between the output light power P of the laser beam emitted from the distal end of the endoscope 4 and the distance D is obtained as shown in table 2 below.
Table 2 relation between output light power P of laser light emitted from distal end of endoscope 4 and distance D
Table 1 is incorporated&Table 2 finally, the relation between the output voltage V of the photodetector 9 and the output optical power P of the laser light emitted from the distal end of the endoscope 4 (i.e., the output voltage V of the photodetector 9 and the actual output optical power of the laser 1In table 3 below), the light source control module 10 fits a P-V curve based on the relationship between the output voltage V of the photodetector 9 and the output light power P of the laser light emitted from the distal end of the endoscope 4.
Table 3 relation between output voltage V of photodetector 9 and output optical power P of laser light emitted from front end of endoscope 4
In this way, the output voltage V of the photodetector 9 plays a role in distance guidance in the system, the output voltage V of the photodetector 9 is input to the light source controller 10, and the light source controller 10 automatically obtains the actual output light power of the laser 1 through the P-V curveThe actual optical power output of the laser 1 is then controlled.
Preferably, according to the endoscopic application, the furthest observation distance is set to Ncm (in this embodiment, n=12 cm), and the output voltage of the photodetector corresponds to V N At this time, the output light power of the laser light emitted from the distal end of the endoscope becomes the maximum value P N ：
For D < N, at this time, according to the P-V curve, the output light power P of the laser emitted from the front end of the endoscope can be obtained;
for D>When N is the same, the output light power of the laser emitted from the front end of the endoscope is uniform to P N ；
Where D is the distance between the distal end face of the actual endoscope 4 and the tissue to be observed, N is the furthest observation distance of the endoscope 4, and V N The output voltage P corresponding to the photodetector 9 when the furthest observation distance of the endoscope 4 is Ncm N The output power of the laser beam emitted from the distal end of the endoscope 4 when the farthest observation distance of the endoscope 4 is Ncm is P, which is the output power of the laser beam emitted from the distal end of the endoscope 4 actually required.
Specifically, the actual optical power of the laser 1 is adjusted by adopting a pulse width modulation method in the technical scheme, and in order not to influence the imaging of the imaging system, the pulse modulation frequency is 10kHz (other frequency values can be selected). At D<In this case, the pulse width modulation duty ratio of the output light power of the laser light emitted from the distal end of the endoscope 4 is P/>*100, wherein P is the output light power of the laser emitted from the front end of the endoscope which is actually needed, < +.>The furthest observation distance for the endoscope is Ncm (in this example, N is preferably 12 cm)The output light power of the laser emitted from the front end of the endoscope is shown in fig. 3.
Specifically, the present solution uses amplitude modulation instead of pulse width modulation to implement adjustment of the actual optical power of the laser 1, see fig. 4.
The present technical solution not only uses pulse width modulation and amplitude modulation methods to realize the adjustment of the actual optical power of the laser 1, but also uses other modulation methods to realize the adjustment of the actual optical power of the laser 1.
According to the technical scheme, the guiding light is added into the light source, the spectroscope is added onto the imaging light path, the guiding light intensity entering the imaging light path is detected through the photoelectric detector, the distance is obtained through calculation and fed back to the system, the output light power can be adjusted in real time, fluorescent imaging is met, meanwhile, the tissue can be prevented from being irradiated by high-power density laser for a long time, and the damage of the laser to biological tissue is reduced; when a fluorescence endoscope is observed near a tissue, since the illumination area is reduced with the light power unchanged, the light power per unit area, that is, the irradiation illuminance is generally increased in a square relationship, but the irradiation illuminance is not required to be too large in fluorescence imaging, and therefore, after the distance is closed, there is a margin for down-regulating the light power.
In the description of the present specification, reference to the terms "one embodiment," "certain embodiments," "illustrative embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiments or examples. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
It is to be understood that the invention is not limited in its application to the examples described above, but is capable of modification and variation in light of the above teachings by those skilled in the art, and that all such modifications and variations are intended to be included within the scope of the appended claims.
Claims (8)
1. The automatic adjustment method for the laser power of the fluorescent navigation endoscope based on the photometric feedback is characterized by comprising the following steps of:
step S1: the laser emitted by the laser and the guiding light emitted by the guiding light source are transmitted through the same guiding light beam and coupled into the endoscope;
step S2: the laser and the guiding light are emitted from the front end of the endoscope and reach the observed tissue, and the exciting light, the fluorescent light and the guiding light reflected by the observed tissue are collected by the endoscope;
step S3: the excitation light is filtered by the filter, and the fluorescence and the guiding light are focused by the lens, wherein the fluorescence is imaged on the camera through the dichroic spectroscope, the guiding light is reflected by the dichroic spectroscope and is incident on the photoelectric detector;
step S4: the photoelectric detector converts the guiding light signal into output voltage and outputs the output voltage to the light source control module;
step S5: the light source control module fits a relation curve between the actual output light power of the laser and the output voltage of the photoelectric detector;
step S6: the light source control module automatically obtains the light power to be output of the laser according to the relation curve of the actual output light power of the laser and the output voltage of the photoelectric detector, and then controls the actual light power output of the laser;
specifically, in the step S5, distances between the front end face of the endoscope and the observed tissue are tested, and the photodetectors correspondingly output different output voltages, so as to obtain a relationship between the output voltages of the photodetectors and the distances between the front end face of the endoscope and the observed tissue; the laser radiation illumination required by the fluorescent imaging of the system isUnder the distances between the front end surfaces of different endoscopes and the observed tissues, the irradiation illuminance E is actually measured, and the output light power of the laser emitted from the front end of the endoscope is adjusted so that the actual measurement value E is equal to +.>Obtaining the relation between the output light power and the distance of the laser emitted from the front end of the endoscope; finally, the relation between the output voltage of the photoelectric detector and the output light power of the laser emitted from the front end of the endoscope is obtained, and the light source control module fits a P-V curve according to the relation between the output voltage of the photoelectric detector and the output light power P of the laser emitted from the front end of the endoscope, namely, the relation curve between the actual output light power of the laser and the output voltage of the photoelectric detector.
2. The automatic laser power adjustment method for fluorescent navigation endoscope based on photometric feedback according to claim 1, wherein the furthest observation distance is set to be N cm and the output voltage of the photoelectric detector corresponds to be V according to the application of the endoscope N At this time, the output light power of the laser light emitted from the distal end of the endoscope becomes the maximum value P N ：
For D < N, at this time, according to the P-V curve, the output light power P of the laser emitted from the front end of the endoscope can be obtained;
for D>When N is the same, the output light power of the laser emitted from the front end of the endoscope is uniform to P N ；
Wherein D is the distance between the front end surface of the actual endoscope and the observed tissue, N is the furthest observation distance of the endoscope, and V N The output voltage corresponding to the photoelectric detector when the furthest observation distance of the endoscope is N cm is P N The output light power of the laser beam emitted from the distal end of the endoscope when the farthest observation distance of the endoscope is N cm is defined as P, and the output light power of the laser beam emitted from the distal end of the endoscope is actually required.
3. The automatic adjustment method for laser power of fluorescent navigation endoscope based on photometric feedback according to claim 1, wherein the adjustment of the actual optical power of the laser is realized by adopting a pulse width modulation method.
4. The automatic adjustment method for laser power of fluorescence navigation endoscope based on photometric feedback according to claim 3, wherein the pulse modulation frequency is 10kHz.
5. The automatic adjustment method of laser power of fluorescence navigation endoscope based on photometric feedback according to claim 4, wherein in D<In this case, the pulse width modulation duty ratio of the output light power of the laser beam emitted from the distal end of the endoscope is P/>*100, wherein P is the output light power of the laser emitted from the front end of the endoscope which is actually needed, < +.>The output light power of the laser beam emitted from the distal end of the endoscope when the farthest observation distance of the endoscope is Ncm.
6. The automatic adjustment method for laser power of fluorescent navigation endoscope based on photometric feedback according to claim 1, wherein the adjustment of the actual optical power of the laser is realized by amplitude modulation.
7. A fluorescence navigation endoscope system based on photometric feedback using the automatic adjustment method of laser power of fluorescence navigation endoscope based on photometric feedback according to any of claims 1-6, characterized by comprising a laser, a guiding light source, a light guide beam, an endoscope, a filter, a lens, a dichroic spectroscope, a camera, a photodetector, a light source control module; the photosensitive surface of the photoelectric detector is positioned on the imaging surface of the guiding light;
the laser emitted by the laser and the guiding light emitted by the guiding light source are transmitted through the same guiding light beam and coupled into the endoscope; the laser and the guiding light are emitted from the front end of the endoscope and reach the observed tissue, the exciting light, the fluorescent light and the guiding light reflected by the observed tissue are collected by the endoscope, wherein the exciting light is filtered by a filter, the fluorescent light and the guiding light are focused by a lens, the fluorescent light is imaged on a camera through a dichroic spectroscope, the guiding light is reflected by the dichroic spectroscope and is incident on a photoelectric detector; the photoelectric detector converts the guiding light signal into output voltage and outputs the output voltage to the light source control module;
the light source control module fits a P-V curve according to the relation between the output voltage of the photoelectric detector and the output light power of laser emitted from the front end of the endoscope; according to the P-V curve, the light source controller automatically obtains the light power to be output by the laser, and then controls the actual light power output of the laser.
8. The fluorescence navigation endoscope system based on photometric feedback of claim 7, wherein the photodetector employs a photodiode.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810227406.1A CN108523819B (en) | 2018-03-20 | 2018-03-20 | Fluorescent navigation endoscope system with photometric feedback and automatic laser power adjustment method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810227406.1A CN108523819B (en) | 2018-03-20 | 2018-03-20 | Fluorescent navigation endoscope system with photometric feedback and automatic laser power adjustment method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108523819A CN108523819A (en) | 2018-09-14 |
| CN108523819B true CN108523819B (en) | 2024-03-22 |
Family
ID=63484747
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810227406.1A Active CN108523819B (en) | 2018-03-20 | 2018-03-20 | Fluorescent navigation endoscope system with photometric feedback and automatic laser power adjustment method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108523819B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109194869A (en) | 2018-10-09 | 2019-01-11 | Oppo广东移动通信有限公司 | Control method, control device, depth camera and electronic device |
| CN109758093B (en) * | 2019-01-31 | 2023-08-25 | 广东欧谱曼迪科技有限公司 | Guiding exposure type fluorescence endoscope system and guiding exposure method |
| CN109744987A (en) * | 2019-01-31 | 2019-05-14 | 广东欧谱曼迪科技有限公司 | A kind of exposure feedback-type fluorescence navigation endoscopic system and exciting light self-regulating method |
| CN112057046A (en) * | 2020-09-10 | 2020-12-11 | 南京诺源医疗器械有限公司 | Tumor fluorescence imaging spectrum diagnostic apparatus |
| CN114052909A (en) * | 2021-12-01 | 2022-02-18 | 辽宁北镜医疗科技有限公司 | Multifunctional navigation system and navigation method in near-infrared fluoroscopy |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102379676A (en) * | 2010-08-31 | 2012-03-21 | 富士胶片株式会社 | Endoscopic light guide and endoscope having the same |
| CN202191264U (en) * | 2011-07-20 | 2012-04-18 | 上海波汇通信科技有限公司 | Endoscope |
| CN102753079A (en) * | 2010-02-10 | 2012-10-24 | 奥林巴斯株式会社 | Fluorescence endoscope device |
| WO2016181077A1 (en) * | 2015-05-12 | 2016-11-17 | Commissariat à l'énergie atomique et aux énergies alternatives | Device and method for observing an object, taking into consideration the distance between the device and the object |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100583250B1 (en) * | 2004-03-05 | 2006-05-24 | 한국전기연구원 | Fluorecence endoscope having improved image detection module |
-
2018
- 2018-03-20 CN CN201810227406.1A patent/CN108523819B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102753079A (en) * | 2010-02-10 | 2012-10-24 | 奥林巴斯株式会社 | Fluorescence endoscope device |
| CN102379676A (en) * | 2010-08-31 | 2012-03-21 | 富士胶片株式会社 | Endoscopic light guide and endoscope having the same |
| CN202191264U (en) * | 2011-07-20 | 2012-04-18 | 上海波汇通信科技有限公司 | Endoscope |
| WO2016181077A1 (en) * | 2015-05-12 | 2016-11-17 | Commissariat à l'énergie atomique et aux énergies alternatives | Device and method for observing an object, taking into consideration the distance between the device and the object |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108523819A (en) | 2018-09-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108523819B (en) | Fluorescent navigation endoscope system with photometric feedback and automatic laser power adjustment method | |
| EP4164338A1 (en) | Endoscopic light source and imaging system | |
| US20130345517A1 (en) | Light source apparatus and endoscope system | |
| US8233039B2 (en) | Microscope image pickup system | |
| EP3146891A1 (en) | Illumination unit and endoscopic apparatus | |
| KR20110094283A (en) | Endoscopic led light source having a feedback control system | |
| US8804120B2 (en) | Fine particle analyzing apparatus and fine particle analyzing method | |
| CN108670172A (en) | Based on fluorescence navigation method of adjustment in the fluorescence navigation system and its art for surveying light feedback | |
| WO2015186384A1 (en) | Endoscope system | |
| US9480405B2 (en) | Photodynamic diagnosis apparatus, photodynamic diagnosis method and device | |
| RU186810U1 (en) | Pulsed laser illuminator | |
| JPH11326210A (en) | Chlorophyll fluorescence measuring instrument | |
| CN109744987A (en) | A kind of exposure feedback-type fluorescence navigation endoscopic system and exciting light self-regulating method | |
| CN210749128U (en) | Exposure feedback type fluorescence navigation endoscope system based on excitation light self-adjustment | |
| JP2007082769A (en) | Sample analysis apparatus | |
| JP2005091701A (en) | Fluorescence microscope and exciting light source control method thereof | |
| CN109758093B (en) | Guiding exposure type fluorescence endoscope system and guiding exposure method | |
| CN217566002U (en) | Endoscope imaging system | |
| JP2009218009A (en) | Light volume control device and solar simulator | |
| JP2014115121A (en) | Microparticle analyzer, and microparticle analysis method | |
| CN115200703A (en) | Light source detection device | |
| CN101784884A (en) | Be used to characterize the method and system of pigmented biological tissue | |
| CN210494018U (en) | Photometry feedback fluorescence endoscope system based on detection signal automatic adjustment | |
| US20130105708A1 (en) | Narrow band fluorophore exciter | |
| JPH09149891A (en) | Fluorescent diagnostic device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |