CN108522949A - A kind of active biological product and its processing method - Google Patents
A kind of active biological product and its processing method Download PDFInfo
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- CN108522949A CN108522949A CN201810008410.9A CN201810008410A CN108522949A CN 108522949 A CN108522949 A CN 108522949A CN 201810008410 A CN201810008410 A CN 201810008410A CN 108522949 A CN108522949 A CN 108522949A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
- A23L13/20—Meat products; Meat meal; Preparation or treatment thereof from offal, e.g. rinds, skins, marrow, tripes, feet, ears or snouts
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
- A23L13/50—Poultry products, e.g. poultry sausages
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/27—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/30—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation
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Abstract
The invention discloses a kind of active biological product and its processing methods, its biological products includes glycosaminoglycan, active collagen and a small amount of water, its processing method includes that cleaning coarse crushing goes periosteum foreigh protein removing washing and sterilizing to be dehydrated centrifugal drying powder, the gartilage powder of active material is rich in product, its maximum temperature processed is no more than the critical denaturation temperature by detecting determining cartilage, process operation process does not contact strong acid and strong base, the gartilage powder of preparation possesses the prototype structure of active material, by being rich in active typeⅡ Collagen, glycosaminoglycan and a small amount of water composition, it can be used for field of health care products, field of food.
Description
Technical field
The present invention relates to field of biological product, more particularly to a kind of active biological product and its processing method.
Background technology
Contain abundant active constituent, other than proteoglycans, also a large amount of glycoprotein such as II type glue in animal cartilage
Former albumen and minerals are the potential sources of II Collagen Type VI.Studies have shown that the functional characteristic of typeⅡ Collagen is by its molecule knot
Influence (the Rigo Hartmann, Bairati, 2002 of structure；Hui Cao, Shi-Ying Xu, 2008).High temperature, radiation, strong acid
Highly basic processing can all destroy the structure of typeⅡ Collagen, influence the performance of its functional characteristic, such as traditional use protease
The technique that hydrolysis extraction, purifying prepare typeⅡ Collagen.Therefore, using a kind of mild and simple method, make in cartilage
Active constituent especially typeⅡ Collagen retains its prototype structure, is given birth to close to its most natural form in animal body
Object activity and functional characteristic utmostly remain, and are of great significance.
Similar patent 201110130611.4 the present invention relates to a kind of method preparing bio-active products, the present invention relates to
A kind of dewatering of the natural organic substance containing bioactive ingredients especially protein, there are antimicrobial medium and
When preferred ionizable salt and equal to or less than at a temperature of being denaturalized, this substance of dry particles form, Zhi Daoshui
Content be reduced to 15% hereinafter, this method does not change the prototype structure of active constituent.
The ionizable salt that above-mentioned Patent Organism biologically active prod is added in preparation process in the form of physical mixed is retained in end
In product so that the natural active matter content of product reduces, meanwhile, the presence of high dose salt, which has human body, in product bears work
With limiting its application in health products, field of food.
Invention content
A kind of active biological product, ingredient include：Glycosaminoglycan, collagen and a small amount of water
A kind of processing method of active biological product, processing method comprise the steps of：
1. cleaning：Animal cartilage tissue is taken, os osseum, residual meat and adipose tissue is removed, is drained after stirring and washing；
2. coarse crushing：Cartilage is cut into 1cm or so sizes；
3. removing periosteum：Cartilage and sodium chloride or potassium chloride, VC will be chopped up with mass ratio 20：1：0.5 to be stirred 1~2 small
When, it is cleaned to 500 μ s/cm of conductivity or less with drinking water；
4. foreigh protein removing：One or more of less salt organic acid solns that 2~5 times of volume cartilage weights (v/w) are added impregnate, dense
Degree control makes cartilaginous tissue relaxation, expansion, stirs 1~2 hour, temperature is controlled at 25 DEG C or less in 0.1~1.0mol/L；
5. cleaning：The impurity for filtering off organic acid soln and elution, is cleaned up with purified water；
6. sterilizing：Cartilage 1-5 times of volume of weight (v/w) 75% alcohol is added, impregnates 1 hour, this process was repeated one time, temperature
Degree control is at 25 DEG C or less；7. being dehydrated：Cartilage 1-5 times of volume of weight (v/w) 90% or more alcohol is added, impregnates 5~30 minutes
To cartilage surface at cartilage is taken out rapidly after white, this process temperature is controlled at 25 DEG C or less；
8. centrifuging：Cartilage is placed in centrifuge, centrifuge dripping alcohol to cartilage loss on drying is less than 85%；
9. drying：Cartilage is dried with vacuum drier to 10% or less moisture；
10. powder processed：Cartilage after drying is crushed with liquid nitrogen frozen, 60~200 mesh screens is crossed, obtains product powder.
Preferably, the less salt organic acid soln in the step 4 is to contain citric acid, malic acid, oxalic acid, acetic acid and winestone
The mixed solution of sour a kind of or several organic acids.
Preferably, step 4 cartilage is aided with supersound process in soaking process.
Preferably, the step 6 is aided with ozone sterilization in sterilization process.
Preferably, step 9 drying process, temperature are not higher than the critical denaturation temperature of animal cartilage.
Preferably, the critical denaturation temperature of the step 11 passes through differential scanning calorimeter, Electronic Speculum, X-ray diffractometer, circle
Dichroscope spectrometer measures.
Advantageous effect：The present invention provides a kind of active biological product and its processing method, biological products include animal
Cartilage, sodium chloride or potassium chloride, VC, processing method include that cleaning-coarse crushing-goes periosteum-foreigh protein removing-cleaning-sterilizing-de-
Water-centrifugation-drying-powder processed, is rich in the gartilage powder of active material in product, and the maximum temperature of processing is no more than true by detection
The critical denaturation temperature of fixed cartilage, process operation process do not contact strong acid and strong base, and the gartilage powder of preparation possesses active material
Prototype structure is formed by being rich in active typeⅡ Collagen, glycosaminoglycan and a small amount of water, can be used for field of health care products, food
Field, the less salt organic acid soln in the step 4 be containing citric acid, malic acid, oxalic acid, acetic acid and tartaric acid it is a kind of or
The mixed solution of several organic acids, step 4 cartilage are aided with supersound process in soaking process, which can be soft by animal
Bone tissue relaxation expansion rapidly at faster speed, improves the processing efficiency of product, the step 6 is aided in sterilization process
Ozone sterilization, the technique can more thoroughly sterilize, step 9 drying process, and temperature is not higher than animal cartilage
Critical denaturation temperature, the critical denaturation temperature of step 11 pass through differential scanning calorimeter, Electronic Speculum, X-ray diffractometer, circle two
Coloured light spectrometer measures, which can effectively prevent animal cartilage to occur protein denaturation in process so that biology system
Product can keep its nutritive value.
Specific implementation mode
To make the technical means, the creative features, the aims and the efficiencies achieved by the present invention be easy to understand, with reference to
Specific implementation mode, the present invention is further explained.
Embodiment 1：
A kind of active biological product, ingredient include：Rich in active typeⅡ Collagen, glycosaminoglycan and a small amount of water.
A kind of processing method of active biological product, processing method comprise the steps of：
1. cleaning：Chicken articular cartilage is taken, os osseum, residual meat and adipose tissue is removed, is drained after stirring and washing；
2. coarse crushing：Cartilage is cut into 1cm or so sizes；
3. removing periosteum：Cartilage and sodium chloride or potassium chloride, VC will be chopped up with mass ratio 20：1：0.5 is stirred 1 hour,
It is cleaned to 500 μ s/cm of conductivity or less with drinking water；
4. foreigh protein removing：One or more of less salt organic acid solns that 2 times of volume cartilage weights (v/w) are added impregnate, concentration
Control is aided with supersound process in 0.1mol/L, makes cartilaginous tissue relaxation, expansion, stirs 1 hour, temperature is controlled at 25 DEG C or less；
5. cleaning：The impurity for filtering off organic acid soln and elution, is cleaned up with purified water；
6. sterilizing：Cartilage 1 times of 75% alcohol of volume (v/w) of weight is added, impregnates 1 hour, is aided with ozone sterilization, this process
It is repeated once, temperature is controlled at 25 DEG C or less；
7. being dehydrated：Cartilage 1 times of 90% or more alcohol of volume (v/w) of weight is added, impregnates 5 minutes to cartilage surface into white
Take out cartilage rapidly afterwards, this process temperature is controlled at 25 DEG C or less；
8. centrifuging：Cartilage is placed in centrifuge, centrifuge dripping alcohol to cartilage loss on drying is less than 85%；
9. drying：Cartilage is dried to moisture 10% with vacuum drier hereinafter, temperature is critical not higher than animal cartilage
Denaturation temperature, critical denaturation temperature are measured by differential scanning calorimeter, Electronic Speculum, X-ray diffractometer, circular dichroism instrument；
10. powder processed：Cartilage after drying is crushed with liquid nitrogen frozen, 60 mesh screens is crossed, obtains product powder.
Embodiment 2：
A kind of active biological product, ingredient include：Rich in active typeⅡ Collagen, glycosaminoglycan and a small amount of water.
A kind of processing method of active biological product, processing method comprise the steps of：
1. cleaning：Chicken articular cartilage is taken, os osseum, residual meat and adipose tissue is removed, is drained after stirring and washing；
2. coarse crushing：Cartilage is cut into 1cm or so sizes；
3. removing periosteum：Cartilage and sodium chloride or potassium chloride, VC will be chopped up with mass ratio 20：1：0.5 is stirred 1 hour,
It is cleaned to 500 μ s/cm of conductivity or less with drinking water；
4. foreigh protein removing：One or more of less salt organic acid solns that 4 times of volume cartilage weights (v/w) are added impregnate, concentration
Control in 0.5mol/L, be aided with supersounds process, make cartilaginous tissue relaxation, expansion, stir 1.5 hours, temperature control 25 DEG C with
Under；
5. cleaning：The impurity for filtering off organic acid soln and elution, is cleaned up with purified water；
6. sterilizing：Cartilage 3 times of 75% alcohol of volume (v/w) of weight are added, impregnates 1 hour, is aided with ozone sterilization, this process
It is repeated once, temperature is controlled at 25 DEG C or less；
7. being dehydrated：Cartilage 3 times of 90% or more alcohol of volume (v/w) of weight are added, impregnate 15 minutes to cartilage surface Cheng Bai
Cartilage is taken out after color rapidly, this process temperature is controlled at 25 DEG C or less；
8. centrifuging：Cartilage is placed in centrifuge, centrifuge dripping alcohol to cartilage loss on drying is less than 85%；
9. drying：Cartilage is dried to moisture 10% with vacuum drier hereinafter, temperature is critical not higher than animal cartilage
Denaturation temperature, critical denaturation temperature are measured by differential scanning calorimeter, Electronic Speculum, X-ray diffractometer, circular dichroism instrument；
10. powder processed：Cartilage after drying is crushed with liquid nitrogen frozen, 120 mesh screens is crossed, obtains product powder.
Embodiment 3：
A kind of active biological product, ingredient include：Rich in active typeⅡ Collagen, glycosaminoglycan and a small amount of water.
A kind of processing method of active biological product, processing method comprise the steps of：
1. cleaning：Fresh chicken articular cartilage is taken, os osseum, residual meat and adipose tissue is removed, is drained after stirring and washing；
2. coarse crushing：Cartilage is cut into 1cm or so sizes；
3. removing periosteum：Cartilage and sodium chloride or potassium chloride, VC will be chopped up with mass ratio 20：1：0.5 is stirred 2 hours,
It is cleaned to 500 μ s/cm of conductivity or less with drinking water；
4. foreigh protein removing：One or more of less salt organic acid solns that 5 times of volume cartilage weights (v/w) are added impregnate, concentration
Control is aided with supersound process in 1.0mol/L, makes cartilaginous tissue relaxation, expansion, stirs 2 hours, temperature is controlled at 25 DEG C or less；
5. cleaning：The impurity for filtering off organic acid soln and elution, is cleaned up with purified water；
6. sterilizing：Cartilage 5 times of 75% alcohol of volume (v/w) of weight are added, impregnates 1 hour, is aided with ozone sterilization, this process
It is repeated once, temperature control is 25 DEG C or less (sterilizing, degreasing, control exothermic reactions)；
7. being dehydrated：Be added cartilage 5 times of 90% or more alcohol of volume (v/w) of weight, impregnate 5~30 minutes to cartilage surface at
Cartilage is taken out after white rapidly, this process temperature is controlled at 25 DEG C or less；
8. centrifuging：Cartilage is placed in centrifuge, centrifuge dripping alcohol to cartilage loss on drying is less than 85%；
9. drying：Cartilage is dried to moisture 10% with vacuum drier hereinafter, temperature is critical not higher than animal cartilage
Denaturation temperature, critical denaturation temperature are measured by differential scanning calorimeter, Electronic Speculum, X-ray diffractometer, circular dichroism instrument；
10. powder processed：Cartilage after drying is crushed with liquid nitrogen frozen, 200 mesh screens is crossed, obtains product powder.
The sample for extracting each embodiment is detected analysis, and is compareed with the prior art, obtains following data：
Can be obtained according to above table data, when implementing 3 parameter of embodiment, active biological product of the present invention and its add
Work method, technological parameter are that total number of bacteria is ＜ 10CFU/g, and active typeⅡ Collagen content is 29%, and glycosaminoglycan contains
Amount is 37%, lipid content 1.2%, and the total number of bacteria of prior art standard is ＜ 1000CFU/g, active II Collagen Type VI egg
Bai Hanliang≤3%, GAG content 25%, lipid content 1.3%, active biological product of the present invention and its processing method
The total number of bacteria of the active biological product of preparation is lower, and protein denaturation degree is lower, GAG content higher, lipid content
It is lower, therefore the present invention has significant superiority.
The present invention provides a kind of active biological product and its processing method, biological products include glycosaminoglycan, activity
Collagen and a small amount of water, processing method include that periosteum-foreigh protein removing-cleaning-sterilizing-dehydration-centrifugation-is gone in cleaning-coarse crushing-
Dry-processed powder, the gartilage powder of active material is rich in product, and the maximum temperature of processing is no more than by detecting determining cartilage
Denaturation temperature, process operation process do not contact strong acid and strong base, and the gartilage powder of preparation possesses the prototype structure of active material, by richness
It is formed containing active collagen, glycosaminoglycan and a small amount of water, can be used for field of health care products, field of food, it is low in the step 4
Salt organic acid soln is containing citric acid, malic acid, oxalic acid, acetic acid and tartaric acid is a kind of or the mixing of several organic acids is molten
Liquid, step 4 cartilage are aided with supersound process in soaking process, which can be by animal cartilage tissue at faster speed
Rapid relaxation expansion, improves the processing efficiency of product, the step 6 is aided with ozone sterilization in sterilization process, the technique energy
Enough more thoroughly to sterilize, step 9 drying process, temperature is not higher than the critical denaturation temperature of animal cartilage, described
The critical denaturation temperature of step 11 is measured, the work by differential scanning calorimeter, Electronic Speculum, X-ray diffractometer, circular dichroism instrument
Artistic skill enough effectively prevent animal cartilage to occur protein denaturation in process so that biological products can keep its nutriture value
Value.
What has been described above is only a preferred embodiment of the present invention, it is noted that for those of ordinary skill in the art
For, without departing from the concept of the premise of the invention, various modifications and improvements can be made, these belong to the present invention
Protection domain.
Claims (7)
1. a kind of active biological product, which is characterized in that its ingredient includes：Glycosaminoglycan, active collagen and a small amount of water.
2. a kind of processing method of active biological product, which is characterized in that its processing method comprises the steps of：
1. cleaning：Animal cartilage tissue is taken, os osseum, residual meat and adipose tissue is removed, is drained after stirring and washing；
2. coarse crushing：Cartilage is cut into 1cm or so sizes；
3. removing periosteum：Cartilage and sodium chloride or potassium chloride, VC will be chopped up with mass ratio 20：1：0.5 is stirred 1~2 hour, uses
Drinking water is cleaned to 500 μ s/cm of conductivity or less；
4. foreigh protein removing：One or more of less salt organic acid solns that 2~5 times of volume cartilage weights (v/w) are added impregnate, concentration control
System makes cartilaginous tissue relaxation, expansion, stirs 1~2 hour, temperature is controlled at 25 DEG C or less in 0.1~1.0mol/L；
5. cleaning：The impurity for filtering off organic acid soln and elution, is cleaned up with purified water；
6. sterilizing：Cartilage 1-5 times of volume of weight (v/w) 75% alcohol is added, impregnates 1 hour, this process was repeated one time, temperature control
System is at 25 DEG C or less；
7. being dehydrated：Cartilage 1-5 times of volume of weight (v/w) 90% or more alcohol is added, impregnates 5~30 minutes to cartilage surface Cheng Bai
Cartilage is taken out after color rapidly, this process temperature is controlled at 25 DEG C or less；
8. centrifuging：Cartilage is placed in centrifuge, centrifuge dripping alcohol to cartilage loss on drying is less than 85%；
9. drying：Cartilage is dried with vacuum drier to 10% or less moisture；
10. powder processed：Cartilage after drying is crushed with liquid nitrogen frozen, 60~200 mesh screens is crossed, obtains product powder.
3. the processing method of active biological product according to claim 2, which is characterized in that the less salt in the step 4
Organic acid soln is containing citric acid, malic acid, oxalic acid, acetic acid and tartaric acid is a kind of or the mixed solution of several organic acids.
4. the processing method of active biological product according to claim 2, which is characterized in that step 4 cartilage is soaking
It is aided with supersound process during bubble.
5. the processing method of active biological product according to claim 2, which is characterized in that the step 6 was sterilizing
It is aided with ozone sterilization in journey.
6. the processing method of active biological product according to claim 2, which is characterized in that step 9 drying process,
Temperature is not higher than the critical denaturation temperature of animal cartilage.
7. the processing method of active biological product according to claim 6, which is characterized in that the critical denaturation of step 11
Temperature is measured by differential scanning calorimeter, Electronic Speculum, X-ray diffractometer, circular dichroism instrument.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111662375A (en) * | 2020-06-11 | 2020-09-15 | 湖北和格复合骨胶原生物科技有限公司 | Preparation method for extracting non-denatured type II collagen from animal cartilage |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102131403A (en) * | 2009-07-27 | 2011-07-20 | 成功大学 | Preparation of high purity collagen |
| CN105132502A (en) * | 2015-09-08 | 2015-12-09 | 四川大学 | Method for extracting pure type II collagen from chicken cartilage |
| CN105147733A (en) * | 2015-07-02 | 2015-12-16 | 嘉兴恒杰生物制药有限公司 | Shark cartilage processing method |
| CN106916870A (en) * | 2017-04-26 | 2017-07-04 | 北京盛美诺生物技术有限公司 | A kind of preparation method of the cartilage extracts of the collagen types of II containing non denatured |
-
2018
- 2018-01-04 CN CN201810008410.9A patent/CN108522949A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102131403A (en) * | 2009-07-27 | 2011-07-20 | 成功大学 | Preparation of high purity collagen |
| CN105147733A (en) * | 2015-07-02 | 2015-12-16 | 嘉兴恒杰生物制药有限公司 | Shark cartilage processing method |
| CN105132502A (en) * | 2015-09-08 | 2015-12-09 | 四川大学 | Method for extracting pure type II collagen from chicken cartilage |
| CN106916870A (en) * | 2017-04-26 | 2017-07-04 | 北京盛美诺生物技术有限公司 | A kind of preparation method of the cartilage extracts of the collagen types of II containing non denatured |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111662375A (en) * | 2020-06-11 | 2020-09-15 | 湖北和格复合骨胶原生物科技有限公司 | Preparation method for extracting non-denatured type II collagen from animal cartilage |
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Application publication date: 20180914 |