CN108522852A - Application of the eucommia leaf extract in preparing the feed addictive for ruminant - Google Patents
Application of the eucommia leaf extract in preparing the feed addictive for ruminant Download PDFInfo
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- CN108522852A CN108522852A CN201810747802.7A CN201810747802A CN108522852A CN 108522852 A CN108522852 A CN 108522852A CN 201810747802 A CN201810747802 A CN 201810747802A CN 108522852 A CN108522852 A CN 108522852A
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- Prior art keywords
- leaf extract
- eucommia leaf
- ruminant
- preparing
- eucommia
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/111—Aromatic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/116—Heterocyclic compounds
- A23K20/121—Heterocyclic compounds containing oxygen or sulfur as hetero atom
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
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- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Birds (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Physiology (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The present invention provides application of the eucommia leaf extract in preparing the feed addictive for ruminant, the present invention, which is experimentally confirmed in ruminant, uses eucommia leaf extract, needs first to handle eucommia leaf extract using coating.Eucommia leaf extract is used for ruminant, eucommia leaf extract can play facilitation to the fermentation of rumen microorganism, and addition 10mg eucommia leaf extracts can improve propionate concentration, reduce rumen ph, and it is horizontal to improve total VFA.So eucommia leaf extract can be used to prepare to the feed addictive of ruminant.
Description
Technical field
The invention belongs to animal feed additive technical fields, and in particular to eucommia leaf extract is dynamic for ruminating in preparation
Application in the feed addictive of object.
Background technology
From eucommia leaf extract is extracted by natural folium cortex eucommiae, to various pathogens and infection of the upper respiratory tract Causative virus etc.
With significant inhibiting effect.Extracorporeal bacteria inhibitor test shows can be to staphylococcus aureus, beta streptococcus, Escherichia coli
There is good inhibiting effect with typhoid bacillus etc..In addition can also strengthen immunity, remove free radical and other effects.In addition, flavones,
Polysaccharide isoreactivity ingredient, it is different to immune system, internal system, central nervous system, the circulatory system and urinary system
The adjustment effect of degree.
Invention content
The object of the present invention is to provide eucommia leaf extract answering in preparing the feed addictive for ruminant
With the present invention mainly studies whether eucommia leaf extract can be degraded by the rumen microorganism in milk cow and it is to the micro- life of cud
The potential impact of object fermentation.
For achieving the above object, the present invention is achieved using following technical proposals：
The present invention provides application of the eucommia leaf extract in preparing the feed addictive for ruminant.
Further：The eucommia leaf extract includes following components：Cortex Eucommiae chlorogenic acid 3%-8%, eucommia bark flavone 8%-
16% and eucommia bark polycose 20%-40%.
Further：The eucommia leaf extract is then added to after coating in feed.
Further：The preparation method of the eucommia leaf extract includes the following steps：It takes folium cortex eucommiae to be extracted with water, extracts
55-65 DEG C of temperature, extraction time 2-4h, is then filtered, and filtrate is taken to be concentrated in vacuo to proportion 1.2 at 60 DEG C, through spraying
The eucommia leaf extract is made in drying tower spray drying.
Further：The mass ratio that the eucommia leaf extract accounts for feed gross mass is 1%-4%.
Further：The substrate that mass ratio containing coarse fodder and fine fodder in the feed is 4: 6.
Further：Coarse fodder is clover in substrate, and fine fodder is the mixture of corn and dregs of beans, corn: the mass ratio of dregs of beans
It is 7: 3.
Compared with prior art, it advantages of the present invention and has the technical effect that：The present invention is experimentally confirmed in ruminant
It is middle to use bile acid and eucommia leaf extract, it needs first to handle bile acid and eucommia leaf extract using coating.By folium cortex eucommiae
Extract is used for ruminant, and eucommia leaf extract can play facilitation to the fermentation of rumen microorganism, improve total VFA
It is horizontal.
Specific implementation mode
Further detailed description is done to technical scheme of the present invention with reference to specific embodiment.
The influence of embodiment 1, in vitro study bile acid and eucommia leaf extract to rumen microbial fermentation
1. materials and methods
1. the acquisition of experimental animal and rumen fluid
3 holstein cows that permanent rumen fistula is housed are selected, daily in 4：30、11：30 and 17：30 feedings, freely
Drinking-water ties formula raising, raises in Agricultural University Of Nanjing Jiangpu farm.Acquisition before experiment rumen fluid is raised in morning, with 4 layers of gauze
Filtering, is placed in the vacuum flask having been warmed up and takes back laboratory operation.
2. experimental design
2.1 In Vitro Fermentations are tested
Experiment is tested using batch, sets up bile acid processing group and eucommia leaf extract processing group separately, bile acid processing group sets 4
A gradient, respectively 0,8.17,16.34,32.68mg bile acids；Eucommia leaf extract in eucommia leaf extract processing group
Additive amount is respectively 0,10,20, (eucommia leaf extract contains chlorogenic acid 7%, eucommia bark polycose 40%, eucommia bark flavone to 40mg
15%).It does not add any substrate in bile acid processing group wherein, and adds the thick ratio of 1g essences in each fermentation flask of eucommia leaf extract group
For 4: 6 substrate, coarse fodder is clover in substrate, and fine fodder is the mixture (corn: dregs of beans=7: 3) of 0.6g corns and dregs of beans.Respectively
Gradient is all provided with 4 repetitions, and the anaerobic culture medium in each fermentation flask is 90mL, and each fermentation flask is inoculated with 10mL rumen fluids.
The preparation of 2.2 anaerobic culture mediums
Culture medium (per 1000m1) (contains CaCl by 0.1mL A liquid in 100ml2·2H2O 13.2g、MnCl2·4H2O
10.0g、CoCl2·6H2O 1.0g、FeCl3·6H2O 8.0g), 200ml B liquid is (per containing NH in 1000ml4HCO34.0g、
NaHCO335.0g) liquid, 200mlC liquid (are hydrated Na per 1000mL containing 19.45g122HPO4、KH2PO46.2g、MgSO4·
7H2O0.6g), 1ml aoxidizes indicator (resazurin solution 0.1%：) and 558.9mlH W/V2O is constituted.It is continually fed into carbon dioxide
To culture medium anaerobism.
2.4 In Vitro Fermentation
The anaerobic culture medium prepared in advance is placed in 39 DEG C of water and is preheated.Rumen fluid is added in 9: 1 ratio, is uniformly mixed
(this process is passed through always CO2, keep anaerobic state).Then it takes 100ml loadings to be always maintained in the fermentation flask of anaerobic state, puts
Enter constant incubator culture, culture is for 24 hours.
2.5 index determining
Measure the pH value and volatile fatty acid (VFA) of fermentation termination.
2. test result
Influence of 2.1 bile acids to rumen microbial fermentation
From table 1 it will be seen that with bile acid amount increase, acetic acid and general volatile fatty acid concentration it is notable under
It drops (P ＜ 0.05), illustrates that bile acid has depression effect to lumen fermentation.
Influence of 1 bile acid of table to lumen fermentation
2.2, influence of the eucommia leaf extract to rumen microbial fermentation
As can be seen from Table 2, addition 10mg eucommia leaf extracts can be improved propionate concentration (P ＜ 0.05), rumen ph is reduced, but into
The additive amount that one step improves eucommia leaf extract then fails to significantly improve rumen microbial fermentation parameter.It compares and is not added with Cortex Eucommiae
Leaf extract group, addition extract group promote rumen microbial fermentation.
Influence of 2 eucommia leaf extract of table to Rumen Fermentation
From in result above it is known that inhibiting effect the addition of bile acid is to rumen microbial fermentation；Add in feed
Suitable eucommia leaf extract is added to play a driving role rumen microbial fermentation, and from the point of view of the concentration of general volatile aliphatic acid,
It is horizontal that addition eucommia leaf extract improves total VFA.
The degradation rate of embodiment 2, Nylon Bag research bile acid and eucommia leaf extract in cud
1. experimental design
1.1 experimental animals and feeding management
3 holstein cows that permanent rumen fistula is housed of selection, daily 4：30、11：30 and 17：30 feedings, freely
Drinking-water ties formula raising, raises in Agricultural University Of Nanjing Jiangpu farm.
2. test procedure
2.1 experiment basic booms
Experiment is divided into 2 groups, every group of 6 repetitions, respectively A groups：Every bag of bile acid 3g；B groups：Every bag of eucommia leaf extract
3g.4 Nylon Bags, wherein bile acid two, folium cortex eucommiae extraction method 2 are thrown into per cow head cud.
2.2 Nylon Bag specifications
Using the nylon cloth of 350 mesh, according to 14X10cm doublings, with terylene sewer, gluing lives the gap sutured, by side
Burning hot to prevent wire drawing on alcolhol burner, drying is weighed.
2.3 operating procedure
Accurately weighing 3g samples is put into Nylon Bag, is fastened with nylon rope.Nylon Bag is uniformly tied up on thick nylon rope, is thrown
Enter in cud, other end rope is tied up on the rumen fistula of ox, unifies to take out for 24 hours afterwards.The Nylon Bag of taking-up is rinsed with water dry
Only, laboratory is transported back, 65.48h is dried in C baking ovens to constant weight, is then weighed.
To ensure the accuracy of experiment, during the experiment, while by the Nylon Bag equipped with bile acid and eucommia ulmoides extracts
(without cud processing), which is put into tap water, impregnates, rinses, to ensure final digestibility not because two extraction methods are molten from Nylon Bag
Go out and influence experimental result, using this data as space management.
3. test result
3 four groups of treatments of the sample of table for 24 hours after degradation rate
The digestibility of bile acid is 70.91% as can be known from Table 3, and the digestibility of eucommia leaf extract is 98.59%.As a result
Illustrate that the two can all be degraded by rumen microorganism.
It can be seen from the above result that the bile acid and eucommia leaf extract of not coated processing can all be degraded in cud.
Wherein, eucommia ulmoides extracts are more easy to be degraded, since eucommia leaf extract is mainly chlorogenic acid, the research in terms of people enteric microorganism
The results show that chlorogenic acid can be degraded by people enteric microorganism, eucommia ulmoides extracts are largely degraded in this experiment, as a result also with people's intestines
Report in road nutrition is consistent.Bile acid shows to be degraded in cud, and it has depression effect to rumen microbial fermentation.
The result is also consistent with research report of the forefathers on people's enteral nutrition, and bile acid itself has inhibitory effect, while itself
It can be degraded by enteric microorganism.
To sum up, if using this two classes feed addictive on ruminant, it is proposed that handled using coating.
The above embodiments are merely illustrative of the technical solutions of the present invention, rather than is limited；Although with reference to aforementioned reality
Applying example, invention is explained in detail, for those of ordinary skill in the art, still can be to aforementioned implementation
Technical solution recorded in example is modified or equivalent replacement of some of the technical features；And these are changed or replace
It changes, the spirit and scope for claimed technical solution of the invention that it does not separate the essence of the corresponding technical solution.
Claims (7)
1. application of the eucommia leaf extract in preparing the feed addictive for ruminant.
2. application of the eucommia leaf extract according to claim 1 in preparing the feed addictive for ruminant,
It is characterized in that：The eucommia leaf extract includes following components：Cortex Eucommiae chlorogenic acid 3%-8%, eucommia bark flavone 8%-16% and Cortex Eucommiae
Polysaccharide 20%-40%.
3. application of the eucommia leaf extract according to claim 1 in preparing the feed addictive for ruminant,
It is characterized in that：The eucommia leaf extract is then added to after coating in feed.
4. application of the eucommia leaf extract according to claim 1 in preparing the feed addictive for ruminant,
It is characterized in that：The preparation method of the eucommia leaf extract includes the following steps：Folium cortex eucommiae is taken to be extracted with water, Extracting temperature
55-65 DEG C, then extraction time 2-4h is filtered, and filtrate is taken to be concentrated in vacuo to proportion 1.2 at 60 DEG C, spray-dried
The eucommia leaf extract is made in tower spray drying.
5. application of the eucommia leaf extract according to claim 1 in preparing the feed addictive for ruminant,
It is characterized in that：The mass ratio that the eucommia leaf extract accounts for feed gross mass is 1%-4%.
6. application of the eucommia leaf extract according to claim 1 in preparing the feed addictive for ruminant,
It is characterized in that：Mass ratio containing coarse fodder and fine fodder in the feed is 4:6 substrate.
7. application of the eucommia leaf extract according to claim 5 in preparing the feed addictive for ruminant,
It is characterized in that：Coarse fodder is clover in substrate, and fine fodder is the mixture of corn and dregs of beans, corn：The mass ratio of dregs of beans is 7：3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810747802.7A CN108522852A (en) | 2018-07-09 | 2018-07-09 | Application of the eucommia leaf extract in preparing the feed addictive for ruminant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810747802.7A CN108522852A (en) | 2018-07-09 | 2018-07-09 | Application of the eucommia leaf extract in preparing the feed addictive for ruminant |
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| Publication Number | Publication Date |
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| CN108522852A true CN108522852A (en) | 2018-09-14 |
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| CN201810747802.7A Pending CN108522852A (en) | 2018-07-09 | 2018-07-09 | Application of the eucommia leaf extract in preparing the feed addictive for ruminant |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110235998A (en) * | 2019-06-21 | 2019-09-17 | 宜兴市天石饲料有限公司 | A kind of preparation method for the compound betaine mixing Cortex Eucommiae element bionic acid agent |
| CN114009591A (en) * | 2021-12-17 | 2022-02-08 | 湖南德诺健康产业集团有限公司 | Natural feed additive for improving growth performance and meat quality of livestock and poultry and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103816209A (en) * | 2014-03-05 | 2014-05-28 | 张家界恒兴生物科技有限公司 | Eu-commin intragastric administration agent for cows and preparation method thereof |
| CN103829038A (en) * | 2014-03-05 | 2014-06-04 | 张家界恒兴生物科技有限公司 | Novel eucommia feed additive and production method thereof |
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2018
- 2018-07-09 CN CN201810747802.7A patent/CN108522852A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103816209A (en) * | 2014-03-05 | 2014-05-28 | 张家界恒兴生物科技有限公司 | Eu-commin intragastric administration agent for cows and preparation method thereof |
| CN103829038A (en) * | 2014-03-05 | 2014-06-04 | 张家界恒兴生物科技有限公司 | Novel eucommia feed additive and production method thereof |
Non-Patent Citations (1)
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| 史仁煌等: "杜仲素对泌乳高峰期奶牛瘤胃发酵和血清指标的影响", 《营养饲料》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110235998A (en) * | 2019-06-21 | 2019-09-17 | 宜兴市天石饲料有限公司 | A kind of preparation method for the compound betaine mixing Cortex Eucommiae element bionic acid agent |
| CN114009591A (en) * | 2021-12-17 | 2022-02-08 | 湖南德诺健康产业集团有限公司 | Natural feed additive for improving growth performance and meat quality of livestock and poultry and application thereof |
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Application publication date: 20180914 |