CN108522500B - A kind of seed cold-induction device and method based on ultrasonic technology - Google Patents
A kind of seed cold-induction device and method based on ultrasonic technology Download PDFInfo
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- CN108522500B CN108522500B CN201810647856.6A CN201810647856A CN108522500B CN 108522500 B CN108522500 B CN 108522500B CN 201810647856 A CN201810647856 A CN 201810647856A CN 108522500 B CN108522500 B CN 108522500B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
Abstract
The invention discloses a kind of seed cold-induction device and method based on ultrasonic technology, wherein seed cold-induction device includes freezing module, control module and power supply module；Freezing module includes two-stage refrigeration system and interior case, and two-stage refrigeration system includes compression refigerating machine and temperature-difference refrigerating piece；Interior case includes heat-insulating cover, controllable temperature cabinet, ultrasonic transducer, coil, stirrer and concentric drain cap；Control module includes single-chip microcontroller, driving circuit and supersonic generator；The side inner wall of the close low-temperature antifreeze liquid of controllable temperature cabinet is the surface with gradient surface energy, and the side inner wall close to temperature-difference refrigerating piece is multiple dimensioned groove structure.The present invention utilizes ultrasonic stimulation seed, improves the germination percentage and germinating energy of seed, promotes children's growth；Using the cavitation of ultrasonic wave, effect of mass transmitting, change the enzyme texture image of seed, improve the activity of enzyme, enhance degeneration-resistant border, antiviral and oxidation resistant ability, improves biological yield.
Description
Technical field
The present invention relates to a kind of freezing storage device and methods, and in particular to be a kind of seed cold-induction device and side
Method.
Background technique
Germ plasm resource is resource very crucial in the reservation of world's species and agricultural production life, and Seed storage is even more in weight
Weight.Nowadays, seed often reserve seed for planting to save year after year by the production production of hybrid seeds, but holding time longer seed, vigor with go out
Bud rate can all be greatly reduced, or even not sprout.It may make that the holding time is longer using deepfreeze technology, seed can also be allowed to keep
Vigor.Although the Cryogenic air that conventional compact formula refrigeration generates can allow seed dormancy, since air conduction coefficient is small, so that
The time that seed Cold pretreatment process needs is very long, needs 3 to 5 hours, since temperature change is very slow, ice crystal
It is generated between seed cell first, it is then intracellular to produce ice crystal again, result in intraor extracellular pressure unbalanced in this way, from
And plasm and cell membrane are destroyed, it may cause cell cracking, death.In addition, compression refrigeration equipment volume is larger, equipment is high
It is expensive, it is unfavorable for carrying out universal use in common peasant household.
The one kind of ultrasonic wave as the Nature energy, crops can efficiently use its kinetic energy, enhance plant cell and enzyme
Vigor, accelerate cell division speed, promotes higher yield of crops, is used widely in recent years in breeding field.Ultrasonic wave tool
There are cavitation, mass transfer, under low-intensity and suitable frequency condition, there is magnetostriction effect and mechanical oscillation effect,
Change enzyme texture image, promotes enzymes biocatalysis activity.
By the inspiration of cell Low-temperature Ice crystallization theory, the present invention is anti-using cold-resistant solution, the low temperature containing MgO nano particle
Freeze liquid and carry out the pretreatment before freezen protective to seed, improves the rate of temperature fall of biological tissue under low temperature, expand freezing model
It encloses, greatly shortens pretreatment time, effectively avoid Interior Seed from forming ice crystal, and then prevent seed cell in refrigerating process
Damage.In addition, the present invention is freezed using temperature-difference refrigerating piece come gradient, the circle of contact arrangement and magnetic force of ultrasonic transducer are utilized
Blender paves seed, uniform temperature, improves seed vitality using the suitable ultrasonic wave of frequency, enhances the resistance of plant
And yield, the economic effect of height to reach the purpose of reducing device volume, reduction investment, when realizing extensive freezen protective seed
Benefit, convenient for popularizing and applying in seeds company.
Summary of the invention
To solve the deficiencies in the prior art, the present invention provides one kind and is based on ultrasonic technology, convenient for general in seeds company
And the seed cold-induction device and method of application.
In order to solve the above technical problems, the technical solution adopted by the present invention is that:
A kind of seed cold-induction device, including freezing module, control module and power supply module；The freezing module includes
Two-stage refrigeration system and interior case, the two-stage refrigeration system include compression refigerating machine and temperature-difference refrigerating piece；The compression refigerating machine
Including evaporator, condenser, compressor and throttle valve；It is characterized by: the interior case includes heat-insulating cover, controllable temperature cabinet, surpasses
Acoustic wave transducer, coil, stirrer and concentric drain cap；The ultrasonic transducer is arranged in described controllable in a manner of the circle of contact
The circumferential direction of incubator body；The coil is arranged in pairs in the controllable temperature cabinet；The stirrer is located at the controllable temperature cabinet
Bottom centre；The concentric drain cap is configured with concentric assembly method in the controllable temperature bottom of box；The temperature-difference refrigerating piece
The circulation of the compression refigerating machine is accessed, temperature-difference refrigerating piece hot end is contacted with the evaporator of the compression refigerating machine, described
Temperature-difference refrigerating piece cold end is contacted with the controllable temperature cabinet exterior；
The control module includes single-chip microcontroller, driving circuit and supersonic generator；The single-chip microcontroller is responsible for generating electricity
Flow timing control signal；The driving circuit exports corresponding electric current timing according to the control signal of single-chip microcontroller；The coil root
Corresponding magnetic field is generated according to the electric current timing of driving circuit output；The stirrer is rotated according to different magnetic field；It is described super
Sonic generator is connect with the ultrasonic transducer；
The side inner wall close to the low-temperature antifreeze liquid of the controllable temperature cabinet is the surface with gradient surface energy,
Side inner wall close to the temperature-difference refrigerating piece is multiple dimensioned groove structure, is configured with metal in the controllable temperature bottom of box
Sintered porous core；
The power supply module is connected with the single-chip microcontroller, the driving circuit and the coil；The power supply module and
The compressor connection；The power supply module is connected with the temperature-difference refrigerating piece；The power supply module and the ultrasonic wave occur
Device, ultrasonic transducer connection.
Using the method for any of the above-described seed cold-induction device freezen protective seed, it is characterised in that: including as follows
Step:
Step 1: being configured to pretreated cold-resistant solution and the low-temperature antifreeze liquid containing MgO nano particle；
Step 2: configured cold-resistant solution is placed into cabinet, cold-resistant processing is carried out to seed using cold-resistant solution,
The time of cold-resistant processing is 10 ~ 15 minutes, has handled emptying cold-resistant solution；
Step 3: the configured low-temperature antifreeze liquid containing MgO nano particle is placed into cabinet, the two-stage is utilized
Refrigeration system and controllable temperature cabinet freeze, while carrying out temperature regulation, and the low temperature containing MgO nano particle is antifreeze
Liquid maintains -45 DEG C~-35 DEG C；
Step 4: being stirred using spiral winding and the stirrer, rotating, containing the low of MgO nano particle described in samming
The ultrasonic transducer concussion of warm anti-icing fluid, the supersonic generator and quadrangle tangential circle arrangement rotates, contains described in samming
There is the low-temperature antifreeze liquid of MgO nano particle and pave the seed, pretreatment in 30 ~ 40 minutes is carried out to the seed；
Step 5: empty the low-temperature antifreeze liquid containing MgO nano particle, by Seed storage in -18 degrees Celsius of freezers,
Carry out air-cooled preservation for a long time.
The cold-resistant solution is made of the following components of mass percent: 3 ~ 5% sodium chloride, 2 ~ 8% ethyl alcohol, 1 ~ 2%
Glycerol, 0.2 ~ 0.8% serine, 0.1 ~ 0.5% trehalose, 0.3 ~ 0.5% ribitol, 0.05 ~ 0.2% laureth
Sodium sulfovinate, remaining is distilled water.
The low-temperature antifreeze liquid containing MgO nano particle includes the component of following mass percent: 0.5 ~ 8% chlorination
Sodium, 5 ~ 12% ethyl alcohol, 7 ~ 10% glycerol, 2 ~ 5% chitin, 0.5 ~ 1.5% alanine, 0.1 ~ 0.5% antierythrite,
The ethanol extract of the dry polypide of 0.05 ~ 0.3% American cockroach, 0.1 ~ 5% MgO nano particle, remaining is distilled water.
The heat-insulating cover is made of heat-insulating material, and thermal coefficient is low, effectively reduces the low temperature containing MgO nano particle
Anti-icing fluid and outside air heat exchange amount, the pretreatment time before further shortening seed cryoprotection effectively avoid in seed
Portion forms ice crystal, and then prevents damage of the seed cell in refrigerating process.
The two-stage refrigeration system is made of compression refigerating machine and temperature-difference refrigerating piece.The compression refigerating machine includes condensation
Device, compressor, throttle valve, medium temperature refrigerant；The temperature-difference refrigerating piece configuration is in the controllable temperature cabinet exterior and the compression
Between refrigeration machine, the circulation loop of the compression refigerating machine is accessed in hot end, and cold end is contacted with the controllable temperature cabinet exterior.It is described
The heat that temperature-difference refrigerating piece absorbs is transmitted to the medium temperature refrigerant, and the heat is transmitted to environment again and is situated between by the medium temperature refrigerant
Matter.
The medium temperature refrigerant can be R12 or R22 etc.；The Cryo Refrigerator agent can be R13, R14, R23 etc..
The controllable temperature bottom of box is configured with metal sintering multi-hole center, and the metal sintering multi-hole center is interior filled with working solution
Body.The side inner wall close to the low-temperature antifreeze liquid of the controllable temperature cabinet is gradient surface, close to the temperature-difference refrigerating
The side inner wall of piece is multiple dimensioned groove structure.The gradient surface is the surface with gradient surface energy, can be with ten
Dioxane base trichlorosilane and octyltrichlorosilane are to spread working medium, prepare gradient table using method of vapor deposition (CVD) etc.
It face can material surface.The multiple dimensioned groove structure is specially multiple dimensioned micro-nano structure --- and wedge-shaped blind hole composition is asymmetric
Groove can use microcavity array with different surfaces energy of high-resolution stereo lithography preparation etc. to constitute.When low
When warm anti-icing fluid temperature increases, the temperature-difference refrigerating piece cold end caloric receptivity increases, hot end thermal discharge increases, the working fluid hair
Raw evaporative phase-change, the working fluid of evaporation are condensed in the gradient surface.The metal sintering multi-hole center is due to high hole
Gap rate so that heat-transfer capability is significantly better than traditional liquid-sucking core, and has excellent average temperature performance, reduces thermal resistance.The gradient
There are gradient surface energy on surface, drop can generate the movement of self-driving type under by nonequilibrium surface tension effects, from
And excluded from the gradient surface in time, it can effectively ensure that and improve condensing heat-exchange performance.The multiple dimensioned groove structure
It can optimize in wall surface upwardly direction and reinforce capillary rise, and the capillary in wall surface downwardly direction is prevented to flow back, from
And reach the one direction transport of liquid from bottom to up, reduce thermal resistance, so that the work area of the temperature-difference refrigerating piece increases, it is single
The external heat output of low-temperature antifreeze liquid increases in the time of position, and temperature reduces, and then achievees the purpose that control temperature.
The ultrasonic transducer has 4n, is divided into n-layer, 4 every layer and is arranged in a manner of quadrangle tangential circle described
The surrounding of cabinet, it is integer, L that the distance on described nearest described cabinet one side of ultrasonic transducer distance, which is L, wherein n > 0 and n,
≥0.The power supply module is connected with the supersonic generator, the ultrasonic transducer.When work, the ultrasonic wave occurs
The power supply of the power supply module is converted to the corresponding high-frequency alternating current of energy converter by device, and the ultrasonic transducer is by the electricity of input
Power is converted into mechanical output (i.e. ultrasonic wave) and passes again, and four strands of ultrasonic waves are centrally formed a vacation in the cabinet
Want to justify, reinforces the rotation for promoting the low-temperature antifreeze liquid containing MgO nano particle.Also, the ultrasonic wave can also make
Seed fullys shake in cabinet, paves in cabinet completely, so that uniform temperature greatly promotes in the cabinet.
The kinetic energy having at the ultrasonic wave can effectively reinforce the physical stimulation to seed, so that it is various in vivo to change it
Physiological activity improves germination percentage and germinating energy, takes root more promotion seedling and increases leaf area and shorten the mature time limit of crop.
At the same time, the ultrasonic wave has the function of cavitation, mass transfer, under low-intensity and suitable frequency condition, has mangneto to stretch
Contracting effect and mechanical oscillation effect, can change enzyme texture image, improve SOD, POD and catalase in plant (
CAT activity) is to enhance degeneration-resistant border, antiviral and oxidation resistant ability, the final energy for improving crops reply natural calamity
Power improves biological yield.
The concentric drain cap includes the N grades of different concentric circles of radius, is configured with concentric assembly method at the cabinet bottom
Portion, the radius of kth grade concentric circles are rk, N-1 grades of concentric circles and the bottom of box are tangent, and wherein N >=1 and N are integer, and 0≤k≤
N-1.When the ultrasonic transducer drives the low-temperature antifreeze liquid rotation containing MgO nano particle, by centrifugal force formula F=
M* ω ^2*r, it is known that the seed with similar features will be rotated with radius very nearly the same and fall into same level-one concentric circles
In, and feature is differed biggish seed and will be rotated and be fallen into concentric circles not at the same level with the biggish radius of difference, wherein F
For centrifugal force, m is seed quality, and ω is angular speed, and r is radius.The concentric drain cap has filtered out the kind with different characteristic
Son, to achieve the purpose that seed selection.
The coil is spiral winding, there is a 2s, and s >=1 and s are integer, and s usually takes 2,3,4, i.e., between the described spiral winding
Angle be respectively 90 °, 60 ° and 45 °.The spiral winding generates opposite magnetic in upper and lower ends after energization according to the right-hand rule
Field, i.e. N pole field and S pole field.The material of the stirrer is permanent magnet, is configured at the bottom of box center.The spiral shell
Spin line circle is connected with the single-chip microcontroller, the driving circuit and the power supply module.4 spiral windings can be used to match
Setting two spiral windings on the quadrangle of the cabinet, i.e. s=2, diagonal line is one group, and spiral winding described in every group is same
When alive, but current direction is on the contrary, the polarity of the magnetic field presented in synchronization is opposite.In this way, utilizing the single-chip microcontroller
Electric current timing control signal is generated, corresponding electric current timing is exported according to the control signal of single-chip microcontroller using the driving circuit,
The rotation of stirrer can be controlled by generating corresponding magnetic field according to the electric current of inflow using the spiral winding, reached and accelerated institute
The purpose of the low-temperature antifreeze liquid rotation containing MgO nano particle is stated, and then reinforces uniform temperature in the cabinet.
The single-chip microcontroller can be the single-chip microcontrollers such as AT89S51；The driving chip of the driving circuit can provide size and
The controllable electric current driving of phase, can be the motor drive ics such as THB6064, TB6560, TB8435, L6219DS.
The power supply module includes the charge and discharge control of lithium battery and the control charge and discharge parameter being connected with its output end
Module.
A method of using seed cold-induction device freezen protective seed, characterized by the following steps:
Step 1: being configured to pretreated cold-resistant solution and the low-temperature antifreeze liquid containing MgO nano particle；
Step 2: configured cold-resistant solution is placed into cabinet, cold-resistant processing is carried out to seed using cold-resistant solution,
The time of cold-resistant processing is 10 ~ 15 minutes, has handled emptying cold-resistant solution；
Step 3: the configured low-temperature antifreeze liquid containing MgO nano particle is placed into cabinet, the two-stage is utilized
Refrigeration system and controllable temperature cabinet freeze, while carrying out temperature regulation, and the low temperature containing MgO nano particle is antifreeze
Liquid maintains -45 DEG C~-35 DEG C；
Step 4: the spiral winding and the stirrer are sufficiently stirred, rotate, containing MgO nano particle described in samming
Low-temperature antifreeze liquid, the supersonic generator and quadrangle tangential circle arrangement the ultrasonic transducer fully shake, rotate,
The low-temperature antifreeze liquid containing MgO nano particle and the seed is paved, 30 ~ 40 minutes pre- is carried out to the seed
Processing；
Step 5: empty the low-temperature antifreeze liquid containing MgO nano particle, by Seed storage in -18 degrees Celsius of freezers,
Carry out air-cooled preservation for a long time.
The cold-resistant solution is made of the following components of mass percent: 3 ~ 5% sodium chloride, 2 ~ 8% ethyl alcohol, 1 ~ 2%
Glycerol, 0.2 ~ 0.8% serine, 0.1 ~ 0.5% trehalose, 0.3 ~ 0.5% ribitol, 0.05 ~ 0.2% laureth
Sodium sulfovinate, remaining is distilled water.
The low-temperature antifreeze liquid containing MgO nano particle includes the component of following mass percent: 0.5 ~ 8% chlorination
Sodium, 5 ~ 12% ethyl alcohol, 7 ~ 10% glycerol, 2 ~ 5% chitin, 0.5 ~ 1.5% alanine, 0.1 ~ 0.5% antierythrite,
The ethanol extract of the dry polypide of 0.05 ~ 0.3% American cockroach, 0.1 ~ 5% MgO nano particle, remaining is distilled water.
Component in the cold-resistant solution has following effect.The serine is a kind of free amino acid, is present in diaphania
In the overwintering young, the metabolism of fat and fatty acid usually can promote, help to maintain immune system, be used as one at low temperature
The natural cold-resistance agent of kind, the hydrogen ion having effectively can inhibit moisture content to freeze in conjunction with moisture content.The trehalose is a kind of
Natural carbohydrate is present in Ou Hua bark beetle larva body, can form unique protective film in cell surface under cryogenic,
It is effectively protected protein molecule invariance inactivation, thus the life process and biological characteristic of the body that sustains life.The ribitol
It is five carbon polyols, is present in Song Abian sawfly Overwintering Larvae body, is a kind of small molecule cryoprotectant, it can be with intracellular water
Part combines, and makes that water is combined to increase into the cell, and free water is reduced, and the water content for participating in freezing is reduced naturally.The laureth
Sodium sulfovinate is a kind of activating agent, and extensive use and daily use chemicals industry can effectively remove dust and other impurities.The sodium chloride is not only
The freezing point of solution can be reduced, it is also ensured that the osmotic pressure and aquatic products cell of vigor liquid are in much the same level, energy
Cell rupture effectively is avoided, while the sodium chloride also has certain bacteria-eliminating efficacy.The ethyl alcohol is added in oxidation resistance liquid, energy
The freezing point of oxidation resistance liquid is greatly reduced, so that oxidation resistance liquid is at -35 DEG C also in liquid phase state.The glycerol is one
The important cryoprotective agent of kind, when the glycerol is added in oxidation resistance liquid, the glycerol can penetrate into the cell, concentration or combination
Intracellular moisture content, and the glycerol also has diluting effect, can reduce the osmotic pressure between oxidation resistance liquid and aquatic products cell tissue.
In the sodium chloride, ethyl alcohol, glycerol, serine, trehalose, ribitol and laureth sodium sulfovinate these types cold-resistant liquid group
Under the coupling divided, so that seed effectively removes internal heterologous ice-nucleus, while the undercooling point of moisture content is reduced, it is suppressed that
Interior Seed moisture content freezes.
The various constituents of the low-temperature antifreeze liquid containing MgO nano particle have following effect.The sodium chloride is
Main salt constituents in water, the component content for regulating and controlling sodium chloride can guarantee Seed storage in similar with primary dis environment molten
In liquid, so that the osmotic pressure of the low-temperature antifreeze liquid containing MgO nano particle and organism are in much the same level, energy
Cell rupture effectively is avoided, in addition, the sodium chloride not only has certain bacteria-eliminating efficacy, the solidification of solution can also be reduced
Point, it is also ensured that.The ethyl alcohol has certain reproducibility, can play to aquatic products oxidation resistant as a kind of organic matter
Protective effect.Joined the vigor liquid of ethyl alcohol simultaneously, freezing point substantially reduces, guarantee vigor liquid -45 DEG C of operating temperature~-
35 DEG C are in liquid phase state.The glycerol is a kind of important cryoprotective agent, while also having moisturizing, high activity, anti-oxidant
Function.The chitin is largely present in the shell of the aquatic products such as shrimp, crab, in Crustaceans content up to 58% ~
85%, a large amount of hydroxyl and amino contained, can effective scavenging hydroxyl, have stronger inoxidizability.Alanine is
One of 20 kinds of amino acid of human body protein is formed, the methyl of side chain plays a part of hydrophobic barrier, prevents moisture content same
Ice-nucleus combines.The antierythrite is a kind of 4 carbon sugar alcohols newly developed, though cannot prevent to freeze, can remain thin in conjunction with water
The structure of born of the same parents' permeability of the membrane and protein, to prevent because of damage caused by freezing.The American cockroach is commonly called as cockroach, earliest
300,005,000 Wan Nianqian are appeared in, one of the insect of most environmental suitability is considered by biologist, dry the second of polypide
Alcohol extracting thing has the function of anti-inflammatory, reparation etc..Not only there is the MgO nano particle magnetism can cooperate the magnetic agitation band
Dynamic seed rotation, and compared with other magnetic nanoparticles, the MgO nano particle can also significantly improve biological under low temperature
The rate of temperature fall of tissue expands freezing range, the thermal conductivity and thermal diffusivity of biological tissue is substantially improved.The sodium chloride,
The ethanol extract and MgO nano particle of the dry polypide of ethyl alcohol, glycerol, chitin, alanine, antierythrite, American cockroach
Under the coupling of these types of low-temperature antifreeze liquid component, so that seed hardly comes to harm under low-temperature antifreeze liquid environment and energy
Enough sufficiently quickly heat exchange, realize optimal organism stored frozen.
Beneficial effect
The seed cold-induction device and method based on ultrasonic technology that the present invention relates to a kind of.The present invention uses cold-resistant solution
And low-temperature antifreeze liquid, the cold-resistant principle of cold-resistant insect is copied, is reduced using cold-resistant ingredient therein by removing heterologous ice-nucleus
Moisture content undercooling point carries out cold-resistant processing to seed, and protection seed preserves from low temperature；Using ultrasonic stimulation seed,
The germination percentage and germinating energy of seed are improved, children's growth is promoted；Using the cavitation of ultrasonic wave, effect of mass transmitting, change the enzyme point of seed
Sub- conformation improves the activity of enzyme, enhances degeneration-resistant border, antiviral and oxidation resistant ability, to improve biological yield；Using described
Two-stage refrigeration system and controllable temperature cabinet freeze, and control the temperature-difference refrigerating piece and the compression using the power supply module
The low-temperature antifreeze liquid temperature containing MgO nano particle is maintained -45 DEG C~-35 DEG C by the power of machine；Utilize the spiral
Coil and the stirrer be sufficiently stirred, rotate, the low-temperature antifreeze liquid containing MgO nano particle described in samming, using described super
Sonic generator and the ultrasonic transducer of quadrangle tangential circle arrangement fully shake, rotate, containing MgO nanometers described in samming
Grain low-temperature antifreeze liquid and pave the seed；It is chosen seeds using the concentric drain cap, and will selected to the seed
High quality seed is put into freezer, carries out air-cooled preservation for a long time.
Detailed description of the invention
Fig. 1 two-stage refrigeration system schematic diagram；
Case explosive view in Fig. 2 seed cold-induction device；
Fig. 3 controllable temperature box-section schematic diagram；
Fig. 4 controllable temperature cabinet and evaporator connection schematic diagram
Fig. 5 spiral winding controls hardware block diagram；
Fig. 6 stirrer rotates schematic diagram；
Fig. 7 ultrasonic transducer quadrangle tangential circle arrangement schematic diagram；
In figure, 1. heat-insulating covers；2. spiral winding；3. controllable temperature cabinet；4. ultrasonic transducer；5. temperature-difference refrigerating piece；
6. stirrer；7. concentric drain cap；Bottom plate in 8.；9. multiple dimensioned groove structure；10. gradient surface；11. ultrasonic wave；12.
Condenser；13. compressor；14. throttle valve；15. evaporator；16. metal sintering multi-hole center.
Specific embodiment
The present invention is described in more detail with reference to the accompanying drawing:
The present invention is based on the seed cold-induction devices of ultrasonic technology, including freezing module, control module and power supply mould
Block.The freezing module includes two-stage refrigeration system and interior case, and the two-stage refrigeration system includes compression refigerating machine and temperature difference system
Cold；The two-stage refrigeration system is made of compression refigerating machine and temperature-difference refrigerating piece.The compression refigerating machine include condenser 12,
Compressor 13, throttle valve 14, medium temperature refrigerant；The configuration of temperature-difference refrigerating piece 5 is in the controllable temperature cabinet exterior and the pressure
Between contraction cold, the circulation loop of the compression refigerating machine is accessed in hot end, and cold end is contacted with 3 outer wall of controllable temperature cabinet.
The heat that the temperature-difference refrigerating piece 5 absorbs is transmitted to the medium temperature refrigerant, and the heat is transmitted to ring again by the medium temperature refrigerant
Border medium.
As shown in case explosive view in Fig. 2 seed cold-induction device, the interior case includes heat-insulating cover 1, controllable temperature cabinet 3, resists
Cold solution, the low-temperature antifreeze liquid containing MgO nano particle, ultrasonic transducer 4, spiral winding 2, stirrer 6, concentric drain cap 7
And temperature-difference refrigerating piece 5.
As shown in Fig. 3 controllable temperature box-section schematic diagram, 3 bottom of controllable temperature cabinet is configured with metal sintering multi-hole center
16, the metal sintering multi-hole center 16 is interior filled with working fluid.The one of the close low-temperature antifreeze liquid of the controllable temperature cabinet 3
Side inner wall is gradient surface 10, and the side inner wall close to the temperature-difference refrigerating piece 5 is multiple dimensioned groove structure 9.Described
Gradient surface is the surface with gradient surface energy, can prepare gradient surface energy material using the methods of vapor deposition (CVD)
Expect surface.The multiple dimensioned groove structure is specially multiple dimensioned micro-nano structure --- and wedge-shaped blind hole forms asymmetric groove,
Microcavity array with different surfaces energy of high-resolution stereo lithography preparation etc. be can use to constitute.When low temperature is antifreeze
When liquid temperature increases, 5 cold end of the temperature-difference refrigerating piece caloric receptivity increases, hot end thermal discharge increases, and the working fluid steams
Phase transformation is sent out, the working fluid of evaporation is condensed in the gradient surface 10.The metal sintering multi-hole center 16 is due to high hole
Gap rate so that heat-transfer capability is significantly better than traditional liquid-sucking core, and has excellent average temperature performance, reduces thermal resistance.The gradient
There are Surface Energy Gradients on surface 10, drop can generate the movement of self-driving type under by nonequilibrium surface tension effects,
To exclude from the gradient surface 10 in time, condensing heat-exchange performance is can effectively ensure that and improved.The multiple dimensioned groove
Structure 9 can optimize in wall surface upwardly direction and reinforce capillary rise, and the capillary in wall surface downwardly direction is prevented to return
Stream reduces thermal resistance, so that the work area of the temperature-difference refrigerating piece 5 to reach the one direction transport of liquid from bottom to up
Increase, the external heat output of low-temperature antifreeze liquid increases in the unit time, and temperature reduces, and then achievees the purpose that control temperature.
As shown in Fig. 4 controllable temperature cabinet and evaporator connection schematic diagram, the configuration of temperature-difference refrigerating piece 5 is in the controllable temperature
Between cabinet exterior and the evaporator 15, hot end is connected with shown evaporator 15 by heat-conducting silicone grease, cold end and it is described can
3 outer wall of temperature control box body is connected by heat-conducting silicone grease.
As shown in Fig. 5 spiral winding control hardware block diagram, when single-chip microcontroller is responsible for the reading of key and generates corresponding electric current
Sequence control signal；Driving circuit exports corresponding electric current timing according to the control signal of single-chip microcontroller；Spiral winding 2 is according to inflow
Electric current generates corresponding magnetic field；Power supply module is responsible for powering to single-chip microcontroller and driving chip.
As shown in Fig. 6 stirrer rotation schematic diagram, two spiral windings 2 on diagonal line are set as one group, every group of spiral
Coil 2 alives simultaneously, but current direction is on the contrary, the polarity of the magnetic field presented in synchronization is opposite.Two groups are controlled respectively
The on-off of electric current and direction in spiral winding 2, so that it may the direction of resultant magnetic field caused by two groups of coil different moments is controlled,
And then control the rotation of stirrer 6.
As shown in Fig. 7 ultrasonic transducer quadrangle tangential circle arrangement schematic diagram, 11 energy converter 4 of ultrasonic wave has 4n, is divided into n
Layer, and is arranged in the surrounding of controllable temperature cabinet 3 in a manner of quadrangle tangential circle by 4 every layer, and wherein n > 0 and n are integer.Work
When, the power supply of power supply module is converted to the corresponding high-frequency alternating current of energy converter by supersonic generator, and ultrasonic transducer 4 will be defeated
The electrical power entered is converted into mechanical output (i.e. ultrasonic wave 11) and passes again, and four strands of ultrasonic waves 11 are at 3 center of controllable temperature cabinet
It forms an imaginary circle, drives the low-temperature antifreeze liquid rotation containing MgO nano particle, so that uniform temperature is significantly in cabinet 3
It is promoted, and seed fullys shake in controllable temperature cabinet 3 in ultrasonic wave 11, is paved in controllable temperature cabinet 3 completely.
Claims (10)
1. a kind of seed cold-induction device based on ultrasonic technology, including freezing module, control module and power supply module；Institute
Stating freezing module includes two-stage refrigeration system and interior case, and the two-stage refrigeration system includes compression refigerating machine and temperature-difference refrigerating piece；
The compression refigerating machine includes evaporator, condenser, compressor and throttle valve；It is characterized by: the interior case include heat-insulating cover,
Controllable temperature cabinet, ultrasonic transducer, coil, stirrer and concentric drain cap；The ultrasonic transducer is in a manner of the circle of contact
It is arranged in the circumferential direction of the controllable temperature cabinet；The coil is arranged in pairs in the controllable temperature cabinet；The stirrer is located at
Controllable temperature bottom of box center；The concentric drain cap is configured with concentric assembly method in the controllable temperature bottom of box；
The temperature-difference refrigerating piece accesses the circulation of the compression refigerating machine, the steaming in temperature-difference refrigerating piece hot end and the compression refigerating machine
Device contact is sent out, the temperature-difference refrigerating piece cold end is contacted with the controllable temperature cabinet exterior；
The control module includes single-chip microcontroller, driving circuit and supersonic generator；When the single-chip microcontroller is responsible for generating electric current
Sequence control signal；The driving circuit exports corresponding electric current timing according to the control signal of single-chip microcontroller；The coil is according to institute
The electric current timing for stating driving circuit output generates corresponding magnetic field；The stirrer is rotated according to different magnetic field；The ultrasonic wave
Generator is connect with the ultrasonic transducer；
The side inner wall of the close low-temperature antifreeze liquid of the controllable temperature cabinet is the surface with gradient surface energy, close to described
The side inner wall of temperature-difference refrigerating piece is multiple dimensioned groove structure, porous configured with metal sintering in the controllable temperature bottom of box
Core；The low-temperature antifreeze liquid is the low-temperature antifreeze liquid of the nano particle containing MgO；The low-temperature antifreeze liquid containing MgO nano particle
Component comprising following mass percent: 0.5 ~ 8% sodium chloride, 5 ~ 12% ethyl alcohol, 7 ~ 10% glycerol, 2 ~ 5% chitin,
The ethanol extract of the dry polypide of 0.5 ~ 1.5% alanine, 0.1 ~ 0.5% antierythrite, 0.05 ~ 0.3% American cockroach,
0.1 ~ 5% MgO nano particle, remaining is distilled water；
The power supply module is connected with the single-chip microcontroller, the driving circuit and the coil；The power supply module and described
Compressor connection；The power supply module is connected with the temperature-difference refrigerating piece；The power supply module and the supersonic generator, institute
State ultrasonic transducer connection.
2. seed cold-induction device according to claim 1, it is characterised in that: the power supply module include lithium battery and with
The charge and discharge control module for the control charge and discharge parameter that its output end is connected.
3. seed cold-induction device according to claim 1, it is characterised in that: the ultrasonic transducer has 4n, point
For n-layer, 4 every layer and the surrounding of the cabinet is arranged in a manner of quadrangle tangential circle, the ultrasonic transducer distance is most
The distance on nearly described cabinet one side is that L, wherein n > 0 and n are integer, L >=0.
4. seed cold-induction device according to claim 1, it is characterised in that: the concentric drain cap include N grades of radiuses not
Same concentric circles, with concentric assembly method configuration in the bottom of box, the radius of kth grade concentric circles is rk, N-1 grades same
Heart circle is tangent with the bottom of box, and wherein N >=1 and N are integer, 0≤k≤N-1.
5. seed cold-induction device according to claim 1, it is characterised in that: the coil is spiral winding, there is 2s,
S >=1 and s are integer.
6. seed cold-induction device according to claim 5, it is characterised in that: s takes 2,3,4, i.e., between the described spiral winding
Angle be respectively 90 °, 60 ° and 45 °.
7. seed cold-induction device according to claim 1, it is characterised in that: the material of stirrer is permanent magnet.
8. using the method for -7 any seed cold-induction device freezen protective seeds according to claim 1, feature exists
In: include the following steps:
Step 1: being configured to pretreated cold-resistant solution and the low-temperature antifreeze liquid containing MgO nano particle；
Step 2: configured cold-resistant solution is placed into cabinet, cold-resistant processing, cold-resistant are carried out to seed using cold-resistant solution
The time of processing is 10 ~ 15 minutes, has handled emptying cold-resistant solution；
Step 3: the configured low-temperature antifreeze liquid containing MgO nano particle is placed into cabinet, freezed using the two-stage
System and controllable temperature cabinet freeze, while carrying out temperature regulation, and the low-temperature antifreeze liquid containing MgO nano particle is tieed up
It holds at -45 DEG C~-35 DEG C；
Step 4: being stirred, being rotated using spiral winding and the stirrer, the low temperature containing MgO nano particle described in samming is prevented
Freeze liquid, the ultrasonic transducer concussion of the supersonic generator and quadrangle tangential circle arrangement rotates, contains described in samming
The low-temperature antifreeze liquid of MgO nano particle and the seed is paved, pretreatment in 30 ~ 40 minutes is carried out to the seed；
Step 5: emptying the low-temperature antifreeze liquid containing MgO nano particle, by Seed storage in -18 degrees Celsius of freezers, carry out
Air-cooled preservation for a long time.
9. according to the method described in claim 8, it is characterized by: the cold-resistant solution by mass percent following components group
At: 3 ~ 5% sodium chloride, 2 ~ 8% ethyl alcohol, 1 ~ 2% glycerol, 0.2 ~ 0.8% serine, 0.1 ~ 0.5% trehalose, 0.3 ~
0.5% ribitol, 0.05 ~ 0.2% laureth sodium sulfovinate, remaining is distilled water.
10. method according to claim 8 or claim 9, it is characterised in that: the low-temperature antifreeze liquid containing MgO nano particle
Component comprising following mass percent: 0.5 ~ 8% sodium chloride, 5 ~ 12% ethyl alcohol, 7 ~ 10% glycerol, 2 ~ 5% chitin,
The ethanol extract of the dry polypide of 0.5 ~ 1.5% alanine, 0.1 ~ 0.5% antierythrite, 0.05 ~ 0.3% American cockroach,
0.1 ~ 5% MgO nano particle, remaining is distilled water.
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| CN110475465B (en) * | 2019-09-18 | 2020-05-22 | 山东大学 | Heat pipe system with jet hole height changing |
| CN110572992B (en) * | 2019-09-18 | 2020-05-19 | 山东大学 | Immersed self-turbulent flow cooling system with four-corner tangential circles |
| CN110631301B (en) * | 2019-10-21 | 2021-06-08 | 山东大学 | Immersed self-turbulent-flow shell-and-tube heat exchanger |
| CN111373900A (en) * | 2020-03-16 | 2020-07-07 | 韩永苗 | Breeding method of cyperus esculentus by cold induction technology |
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| CN1030194A (en) * | 1988-05-18 | 1989-01-11 | 化学工业部炭黑工业研究设计所 | Ultrasonic electromagnetic separating apparatus |
| CN106258070A (en) * | 2016-08-10 | 2017-01-04 | 国俊伍 | A kind of seed freezing storage device and method |
| CN107551920A (en) * | 2016-07-01 | 2018-01-09 | 中国科学院声学研究所 | A kind of multiple dimensioned mixing machine and mixing method |
| CN207271177U (en) * | 2017-09-11 | 2018-04-27 | 官爱平 | Ultrasonic wave agitating device |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1030194A (en) * | 1988-05-18 | 1989-01-11 | 化学工业部炭黑工业研究设计所 | Ultrasonic electromagnetic separating apparatus |
| CN107551920A (en) * | 2016-07-01 | 2018-01-09 | 中国科学院声学研究所 | A kind of multiple dimensioned mixing machine and mixing method |
| CN106258070A (en) * | 2016-08-10 | 2017-01-04 | 国俊伍 | A kind of seed freezing storage device and method |
| CN207271177U (en) * | 2017-09-11 | 2018-04-27 | 官爱平 | Ultrasonic wave agitating device |
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