CN108522500A - A kind of cold-induced device and method of seed based on ultrasonic technology - Google Patents
A kind of cold-induced device and method of seed based on ultrasonic technology Download PDFInfo
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- CN108522500A CN108522500A CN201810647856.6A CN201810647856A CN108522500A CN 108522500 A CN108522500 A CN 108522500A CN 201810647856 A CN201810647856 A CN 201810647856A CN 108522500 A CN108522500 A CN 108522500A
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- temperature
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- seed
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- nano particles
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
Abstract
The invention discloses a kind of cold-induced device and methods of the seed based on ultrasonic technology, and the wherein cold-induced device of seed includes freezing module, control module and power supply module；It includes two-stage refrigeration system and interior case to freeze module, and two-stage refrigeration system includes compression refigerating machine and temperature-difference refrigerating piece；Interior case includes heat-insulating cover, controllable temperature babinet, ultrasonic transducer, coil, stirrer and concentric drain cap；Control module includes microcontroller, driving circuit and supersonic generator；The side internal face of the close low-temperature antifreeze liquid of controllable temperature babinet is the surface with gradient surface energy, and the side internal face close to temperature-difference refrigerating piece is multiple dimensioned groove structure.The present invention utilizes ultrasonic stimulation seed, improves the germination percentage and germinating energy of seed, promotes children's growth；Using the cavitation of ultrasonic wave, effect of mass transmitting, change the enzyme texture image of seed, improve the activity of enzyme, enhance degeneration-resistant border, antiviral and oxidation resistant ability, improves biological yield.
Description
Technical field
The present invention relates to a kind of freezing storage device and methods, and in particular to be a kind of cold-induced device of seed and side
Method.
Background technology
Germ plasm resource is world's species reservation and resource very crucial in agricultural production life, and Seed storage is even more in weight
Weight.Nowadays, seed often reserve seed for planting to preserve year after year by the production production of hybrid seeds, but holding time longer seed, vigor with go out
Bud rate can all be greatly reduced, or even not sprout.It may make that the holding time is longer using deepfreeze technology, also allow for seed holding
Vigor.Although the Cryogenic air that conventional compact formula refrigeration generates can allow seed dormancy, since air conduction coefficient is small so that
The time that seed Cold pretreatment process needs is very long, needs 3 to 5 hours, since temperature change is very slow, ice crystal
It is generated between seed cell first, it is then intracellular to produce ice crystal again, result in intraor extracellular pressure unbalanced in this way, from
And plasm and cell membrane are destroyed, cell cracking, death may be caused.In addition, compression refrigeration equipment volume is larger, equipment is high
It is expensive, it is unfavorable for carrying out universal use in common peasant household.
The one kind of ultrasonic wave as the Nature energy, crops can efficiently use its kinetic energy, enhance plant cell and enzyme
Vigor, accelerate cell division speed, promotes higher yield of crops, is used widely in recent years in breeding field.Ultrasonic wave has
There are cavitation, mass transfer, under low-intensity and suitable frequency condition, there is magnetostriction effect and mechanical oscillation effect,
Change enzyme texture image, promotes enzymes biocatalysis activity.
It is inspired by cell Low-temperature Ice crystallization theory, the present invention is anti-using cold-resistant solution, the low temperature containing MgO nano particles
Freeze liquid and carry out the pretreatment before freezen protective to seed, improves the rate of temperature fall of biological tissue under low temperature, expand freezing model
It encloses, greatly shortens pretreatment time, effectively avoid Interior Seed from forming ice crystal, and then prevent seed cell in refrigerating process
Damage.In addition, the present invention is freezed using temperature-difference refrigerating piece come gradient, the circle of contact arrangement and magnetic force of ultrasonic transducer are utilized
Blender paves seed, uniform temperature, the resistance for improving seed vitality using the suitable ultrasonic wave of frequency, enhancing plant
And yield, to reach the purpose of reducing device volume, reduction is invested, the economic effect of height when realizing extensive freezen protective seed
Benefit, convenient for popularizing and applying in seeds company.
Invention content
To solve the deficiencies in the prior art, the present invention provides one kind and being based on ultrasonic technology, convenient for general in seeds company
And the cold-induced device and method of seed of application.
In order to solve the above technical problems, the technical solution adopted by the present invention is：
A kind of cold-induced device of seed, including freezing module, control module and power supply module；The freezing module includes two-stage
Refrigeration system and interior case, the two-stage refrigeration system include compression refigerating machine and temperature-difference refrigerating piece；The compression refigerating machine includes
Evaporator, condenser, compressor and throttle valve；It is characterized in that：The interior case includes heat-insulating cover, controllable temperature babinet, ultrasonic wave
Energy converter, coil, stirrer and concentric drain cap；The ultrasonic transducer is arranged in the controllable incubator in a manner of the circle of contact
The circumferential direction of body；The coil is arranged in pairs in the controllable temperature babinet；The stirrer is located at the controllable temperature bottom of box
Center；The concentric drain cap is configured with concentric assembly method in the controllable temperature bottom of box；The temperature-difference refrigerating piece access
The cycle of the compression refigerating machine, temperature-difference refrigerating piece hot junction are contacted with the evaporator of the compression refigerating machine, the temperature difference
Cooling piece cold end is contacted with the controllable temperature cabinet exterior；
The control module includes microcontroller, driving circuit and supersonic generator；When the microcontroller is responsible for generating electric current
Sequence control signal；The driving circuit exports corresponding electric current sequential according to the control signal of microcontroller；The coil is according to institute
The electric current sequential for stating driving circuit output generates corresponding magnetic field；The stirrer is rotated according to different magnetic field；The ultrasonic wave
Generator is connect with the ultrasonic transducer；
The side internal face close to the low-temperature antifreeze liquid of the controllable temperature babinet is the surface with gradient surface energy, close
The side internal face of the temperature-difference refrigerating piece is multiple dimensioned groove structure, and metal sintering is configured in the controllable temperature bottom of box
Multi-hole center；
The power supply module is connected with the microcontroller, the driving circuit and the coil；The power supply module and described
Compressor connects；The power supply module is connected with the temperature-difference refrigerating piece；The power supply module and the supersonic generator, institute
State ultrasonic transducer connection.
Using the method for any of the above-described cold-induced device freezen protective seed of seed, it is characterised in that：Including as follows
Step：
Step 1: being configured to pretreated cold-resistant solution and the low-temperature antifreeze liquid containing MgO nano particles；
Step 2: the cold-resistant solution configured is placed into babinet, cold-resistant processing, cold-resistant are carried out to seed using cold-resistant solution
The time of processing is 10 ~ 15 minutes, has handled emptying cold-resistant solution；
Step 3: the low-temperature antifreeze liquid containing MgO nano particles configured is placed into babinet, freezed using the two-stage
System and controllable temperature babinet freeze, and are carried out at the same time temperature regulation and control, and the low-temperature antifreeze liquid containing MgO nano particles is tieed up
It holds at -45 DEG C~-35 DEG C；
Step 4: being stirred, being rotated using spiral winding and the stirrer, the low temperature containing MgO nano particles described in samming is prevented
Freeze liquid, the ultrasonic transducer concussion of the supersonic generator and quadrangle tangential circle arrangement rotates, contains described in samming
The low-temperature antifreeze liquid of MgO nano particles and the seed is paved, pretreatment in 30 ~ 40 minutes is carried out to the seed；
Step 5: the emptying low-temperature antifreeze liquid containing MgO nano particles is carried out by Seed storage in -18 degrees Celsius of freezers
Air-cooled preservation for a long time.
The cold-resistant solution is made of the following components of mass percent：3 ~ 5% sodium chloride, 2 ~ 8% ethyl alcohol, 1 ~ 2%
Glycerine, 0.2 ~ 0.8% serine, 0.1 ~ 0.5% trehalose, 0.3 ~ 0.5% ribitol, 0.05 ~ 0.2% laureth
Sodium sulfovinate, remaining is distilled water.
The low-temperature antifreeze liquid containing MgO nano particles includes the component of following mass percent：0.5 ~ 8% chlorination
Sodium, 5 ~ 12% ethyl alcohol, 7 ~ 10% glycerine, 2 ~ 5% chitin, 0.5 ~ 1.5% alanine, 0.1 ~ 0.5% antierythrite,
The ethanol extract of 0.05 ~ 0.3% American cockroach drying polypide, 0.1 ~ 5% MgO nano particles, remaining is distilled water.
The heat-insulating cover is made of heat-insulating material, and thermal coefficient is low, effectively reduces the low temperature containing MgO nano particles
Anti-icing fluid and extraneous air heat exchange amount, further shorten the pretreatment time before seed cryoprotection, effectively avoid in seed
Portion forms ice crystal, and then prevents damage of the seed cell in refrigerating process.
The two-stage refrigeration system is made of compression refigerating machine and temperature-difference refrigerating piece.The compression refigerating machine includes condensation
Device, compressor, throttle valve, medium temperature refrigerant；The temperature-difference refrigerating piece configuration is in the controllable temperature cabinet exterior and the compression
Between refrigeration machine, the circulation loop of the compression refigerating machine is accessed in hot junction, and cold end is contacted with the controllable temperature cabinet exterior.It is described
The heat that temperature-difference refrigerating piece absorbs is transmitted to the medium temperature refrigerant, and the heat is transmitted to environment again and is situated between by the medium temperature refrigerant
Matter.
The medium temperature refrigerant can be R12 or R22 etc.；The Cryo Refrigerator agent can be R13, R14, R23 etc..
The controllable temperature bottom of box is configured with metal sintering multi-hole center, and the metal sintering multi-hole center is interior filled with working solution
Body.The side internal face close to the low-temperature antifreeze liquid of the controllable temperature babinet is gradient surface, close to the temperature-difference refrigerating
The side internal face of piece is multiple dimensioned groove structure.The gradient surface is the surface with gradient surface energy, can be with ten
Dioxane base trichlorosilane and octyltrichlorosilane are diffusion working medium, prepare gradient table using method of vapor deposition (CVD) etc.
It face can material surface.The multiple dimensioned groove structure is specially multiple dimensioned micro-nano structure --- and wedge-shaped blind hole composition is asymmetric
Groove can be constituted using microcavity array with different surfaces energy of high-resolution stereo lithography preparation etc..When low
When warm anti-icing fluid temperature increases, the temperature-difference refrigerating piece cold end caloric receptivity increases, hot junction thermal discharge increases, the working fluid hair
Raw evaporative phase-change, the working fluid of evaporation are condensed in the gradient surface.The metal sintering multi-hole center is due to high hole
Gap rate so that heat-transfer capability is significantly better than traditional liquid-sucking core, and has excellent average temperature performance, reduces thermal resistance.The gradient
There are gradient surface energies, drop will produce self-driving type under by nonequilibrium surface tension effects and be moved on surface, from
And excluded from the gradient surface in time, it can effectively ensure that and improve condensing heat-exchange performance.The multiple dimensioned groove structure
It can optimize in wall surface upwardly direction and reinforce capillary rise, and the capillary in wall surface downwardly direction is prevented to flow back, from
And reach the one direction transport of liquid from bottom to up, reduce thermal resistance so that the work area of the temperature-difference refrigerating piece increases, single
The external heat output of low-temperature antifreeze liquid increases in the time of position, and temperature reduces, and then achievees the purpose that control temperature.
The ultrasonic transducer has 4n, is divided into n-layer, 4 every layer and is arranged in a manner of quadrangle tangential circle described
The surrounding of babinet, it is integer, L that the distance on described nearest described babinet one side of ultrasonic transducer distance, which is L, wherein n ＞ 0 and n,
≥0.The power supply module is connected with the supersonic generator, the ultrasonic transducer.When work, the ultrasonic wave occurs
The power supply of the power supply module is converted to the corresponding high-frequency alternating current of energy converter by device, and the ultrasonic transducer is by the electricity of input
Power is converted into mechanical output（That is ultrasonic wave）It passes again, four strands of ultrasonic waves are centrally formed a vacation in the babinet
Want to justify, reinforces the rotation for promoting the low-temperature antifreeze liquid containing MgO nano particles.Also, the ultrasonic wave can also make
Seed fully shaking in babinet, is paved in babinet completely, so that uniform temperature greatly promotes in the babinet.
The kinetic energy having at the ultrasonic wave can effectively reinforce the physical stimulation to seed, various in vivo to change it
Physiological activity improves germination percentage and germinating energy, takes root promotion seedling more and increases leaf area and shorten the ripe time limit of crop.
At the same time, the ultrasonic wave has the function of cavitation, mass transfer, under low-intensity and suitable frequency condition, has mangneto to stretch
Contracting effect and mechanical oscillation effect, can change enzyme texture image, improve SOD, POD and catalase in plant (
CAT activity) is to enhance degeneration-resistant border, antiviral and oxidation resistant ability, the final energy for improving crops reply natural calamity
Power improves biological yield.
The concentric drain cap includes the N grades of different concentric circles of radius, is configured at the babinet bottom with concentric assembly method
The radius in portion, kth grade concentric circles is rk, N-1 grades of concentric circles and the bottom of box are tangent, and wherein N >=1 and N is integer, and 0≤k≤
N-1.When the ultrasonic transducer drives the low-temperature antifreeze liquid rotation containing MgO nano particles, by centrifugal force formula F=
M* ω ^2*r, it is known that the seed with similar features will be rotated with radius very nearly the same and fall into same level-one concentric circles
In, and feature is differed larger seed and will be rotated and fallen into concentric circles not at the same level with the larger radius of difference, wherein F
For centrifugal force, m is seed quality, and ω is angular speed, and r is radius.The concentric drain cap has filtered out the kind with different characteristic
Son, to achieve the purpose that seed selection.
The coil is spiral winding, there is a 2s, and s >=1 and s are integer, and s usually takes 2,3,4, i.e., between the described spiral winding
Angle be respectively 90 °, 60 ° and 45 °.The spiral winding generates opposite magnetic after energization according to the right-hand rule in upper and lower ends
Field, i.e. N pole fields and S pole fields.The material of the stirrer is permanent magnet, is configured at the bottom of box center.The spiral shell
Spin line circle is connected with the microcontroller, the driving circuit and the power supply module.It can be matched using 4 spiral windings
Two spiral windings set on the quadrangle of the babinet, i.e. s=2, diagonal line are one group, and spiral winding is same described in every group
When alive, but current direction is on the contrary, the polarity of the magnetic field presented in synchronization is opposite.In this way, utilizing the microcontroller
Electric current timing control signal is generated, corresponding electric current sequential is exported according to the control signal of microcontroller using the driving circuit,
The rotation of stirrer can be controlled by generating corresponding magnetic field according to the electric current of inflow using the spiral winding, reached and accelerated institute
The purpose of the low-temperature antifreeze liquid rotation containing MgO nano particles is stated, and then reinforces uniform temperature in the babinet.
The microcontroller can be the microcontrollers such as AT89S51；The driving chip of the driving circuit can provide size and
The controllable electric current driving of phase, can be the motor drive ics such as THB6064, TB6560, TB8435, L6219DS.
The power supply module includes the charge and discharge control of lithium battery and the control charge and discharge parameter being connected with its output end
Module.
A method of using the cold-induced device freezen protective seed of seed, it is characterised in that：Include the following steps：
Step 1: being configured to pretreated cold-resistant solution and the low-temperature antifreeze liquid containing MgO nano particles；
Step 2: the cold-resistant solution configured is placed into babinet, cold-resistant processing, cold-resistant are carried out to seed using cold-resistant solution
The time of processing is 10 ~ 15 minutes, has handled emptying cold-resistant solution；
Step 3: the low-temperature antifreeze liquid containing MgO nano particles configured is placed into babinet, freezed using the two-stage
System and controllable temperature babinet freeze, and are carried out at the same time temperature regulation and control, and the low-temperature antifreeze liquid containing MgO nano particles is tieed up
It holds at -45 DEG C~-35 DEG C；
Step 4: the spiral winding and the stirrer be sufficiently stirred, rotate, described in samming containing the low of MgO nano particles
Warm anti-icing fluid, the supersonic generator and the ultrasonic transducer fully shaking of quadrangle tangential circle arrangement, rotation, samming institute
It states the low-temperature antifreeze liquid containing MgO nano particles and paves the seed, 30 ~ 40 minutes pre- places are carried out to the seed
Reason；
Step 5: the emptying low-temperature antifreeze liquid containing MgO nano particles is carried out by Seed storage in -18 degrees Celsius of freezers
Air-cooled preservation for a long time.
The cold-resistant solution is made of the following components of mass percent：3 ~ 5% sodium chloride, 2 ~ 8% ethyl alcohol, 1 ~ 2%
Glycerine, 0.2 ~ 0.8% serine, 0.1 ~ 0.5% trehalose, 0.3 ~ 0.5% ribitol, 0.05 ~ 0.2% laureth
Sodium sulfovinate, remaining is distilled water.
The low-temperature antifreeze liquid containing MgO nano particles includes the component of following mass percent：0.5 ~ 8% chlorination
Sodium, 5 ~ 12% ethyl alcohol, 7 ~ 10% glycerine, 2 ~ 5% chitin, 0.5 ~ 1.5% alanine, 0.1 ~ 0.5% antierythrite,
The ethanol extract of 0.05 ~ 0.3% American cockroach drying polypide, 0.1 ~ 5% MgO nano particles, remaining is distilled water.
Component in the cold-resistant solution has following effect.The serine is a kind of free amino acid, is present in diaphania
It in the overwintering young, can usually promote the metabolism of fat and aliphatic acid, help to maintain immune system, be used as one at low temperature
The natural cold-resistance agent of kind, the hydrogen ion carried can be combined with moisture content, effectively moisture content be inhibited to freeze.The trehalose is a kind of
Natural carbohydrate is present in Ou Hua bark beetle larva bodies, can form unique protective film in cell surface under cryogenic,
It is effectively protected protein molecule invariance inactivation, to the life process and biological characteristic of the body that sustains life.The ribitol
It is five carbon polyols, is present in loose Ah flat sawfly Overwintering Larvae body, is a kind of small molecule cryoprotectant, it can be with intracellular water
Part combines, and makes that water is combined to increase into the cell, and free water is reduced, and the water content for participating in freezing is reduced naturally.The laureth
Sodium sulfovinate is a kind of activating agent, and extensive use and daily use chemicals industry can effectively remove dust and other impurities.The sodium chloride is not only
The solidification point of solution can be reduced, it is also ensured that the osmotic pressure of vigor liquid is in much the same level, energy with aquatic products cell
Cell rupture is effectively avoided, while the sodium chloride also has certain bacteria-eliminating efficacy.The ethyl alcohol is added in oxidation resistance liquid, energy
The solidification point of oxidation resistance liquid is greatly reduced so that oxidation resistance liquid is at -35 DEG C also in liquid phase state.The glycerine is one
The important cryoprotective agent of kind, when the glycerine is added in oxidation resistance liquid, the glycerine can penetrate into the cell, concentration or combination
Intracellular moisture content, and the glycerine also has diluting effect, can reduce the osmotic pressure between oxidation resistance liquid and aquatic products cell tissue.
In the sodium chloride, ethyl alcohol, glycerine, serine, trehalose, ribitol and laureth sodium sulfovinate these types cold-resistant liquid group
Under the coupling divided so that seed effectively removes internal heterologous ice-nucleus, while reducing the undercooling point of moisture content, it is suppressed that
Interior Seed moisture content freezes.
The various constituents of the low-temperature antifreeze liquid containing MgO nano particles have following effect.The sodium chloride is
Main salt constituents in water, regulating and controlling the component content of sodium chloride can ensure Seed storage in similar with primary dis environment molten
In liquid so that the osmotic pressure of the low-temperature antifreeze liquid containing MgO nano particles is in much the same level, energy with organism
Cell rupture effectively is avoided, in addition, the sodium chloride not only has certain bacteria-eliminating efficacy, the solidification of solution can also be reduced
Point, it is also ensured that.The ethyl alcohol has certain reproducibility, can be played to aquatic products oxidation resistant as a kind of organic matter
Protective effect.Add the vigor liquid of ethyl alcohol simultaneously, solidification point substantially reduces, ensure vigor liquid -45 DEG C of operating temperature~-
35 DEG C are in liquid phase state.The glycerine is a kind of important cryoprotective agent, while also having moisturizing, high activity, anti-oxidant
Function.The chitin is largely present in the shell of the aquatic products such as shrimp, crab, in Crustaceans content up to 58% ~
85%, a large amount of hydroxyl and amino contained, can effective scavenging hydroxyl, have stronger inoxidizability.Alanine is
One of 20 kinds of amino acid of human body protein is formed, the methyl of side chain plays a part of hydrophobic barrier, prevents moisture content same
Ice-nucleus combines.The antierythrite is a kind of 4 carbon sugar alcohols newly developed can be combined though cannot prevent to freeze with water, remain thin
The structure of born of the same parents' permeability of the membrane and protein, to prevent being damaged caused by icing.The American cockroach is commonly called as cockroach, earliest
Before appearing in 300,015,000 years, it is considered one of the insect of most environmental suitability by biologist, dries the second of polypide
Alcohol extracting thing has the function of anti-inflammatory, reparation etc..The MgO nano particles not only there is magnetism can coordinate the magnetic agitation band
Dynamic seed rotation, and compared with other magnetic nanoparticles, the MgO nano particles can also significantly improve biological under low temperature
The rate of temperature fall of tissue expands freezing range, the thermal conductivity and thermal diffusivity of biological tissue is substantially improved.The sodium chloride,
Ethyl alcohol, glycerine, chitin, alanine, antierythrite, American cockroach dry the ethanol extract and MgO nano particles of polypide
Under the coupling of these types of low-temperature antifreeze liquid component so that seed hardly comes to harm under low-temperature antifreeze liquid environment and energy
Enough fully quickly heat exchange, realize optimal organism stored frozen.
Advantageous effect
The present invention relates to a kind of cold-induced device and method of the seed based on ultrasonic technology.The present invention is using cold-resistant solution and low
Warm anti-icing fluid copies the cold-resistant principle of cold-resistant insect, and moisture content is reduced by removing heterologous ice-nucleus using cold-resistant ingredient therein
Undercooling point carries out cold-resistant processing to seed, and protection seed preserves from low temperature；Using ultrasonic stimulation seed, improve
The germination percentage and germinating energy of seed promote children's growth；Using the cavitation of ultrasonic wave, effect of mass transmitting, change the enzyme molecule structure of seed
As improving the activity of enzyme, enhancing degeneration-resistant border, antiviral and oxidation resistant ability, to improve biological yield；Utilize the two-stage
Refrigeration system and controllable temperature babinet freeze, and the temperature-difference refrigerating piece and the compressor are controlled using the power supply module
The low-temperature antifreeze liquid temperature containing MgO nano particles is maintained -45 DEG C~-35 DEG C by power；Utilize the spiral winding
It is sufficiently stirred, rotates with the stirrer, the low-temperature antifreeze liquid containing MgO nano particles described in samming, utilizing the ultrasonic wave
The ultrasonic transducer fully shaking of generator and quadrangle tangential circle arrangement, contains MgO nano particles described in samming at rotation
Low-temperature antifreeze liquid and pave the seed；It is chosen seeds to the seed using the concentric drain cap, and high-quality by what is selected
Seed is put into freezer, carries out air-cooled preservation for a long time.
Description of the drawings
Fig. 1 two-stage refrigeration system schematic diagrames；
Case explosive view in the cold-induced device of Fig. 2 seeds；
Fig. 3 controllable temperature box-section schematic diagrames；
Fig. 4 controllable temperatures babinet and evaporator connection diagram
Fig. 5 spiral windings control hardware block diagram；
Fig. 6 stirrers rotate schematic diagram；
Fig. 7 ultrasonic transducer quadrangle tangential circle arrangement schematic diagrams；
In figure, 1. heat-insulating covers；2. spiral winding；3. controllable temperature babinet；4. ultrasonic transducer；5. temperature-difference refrigerating piece；6. stirring
Mix son；7. concentric drain cap；Bottom plate in 8.；9. multiple dimensioned groove structure；10. gradient surface；11. ultrasonic wave；12. condensation
Device；13. compressor；14. throttle valve；15. evaporator；16. metal sintering multi-hole center.
Specific implementation mode
The present invention is described in more detail below in conjunction with the accompanying drawings：
The present invention is based on the cold-induced devices of the seed of ultrasonic technology, including freezing module, control module and power supply module.Institute
It includes two-stage refrigeration system and interior case to state freezing module, and the two-stage refrigeration system includes compression refigerating machine and temperature-difference refrigerating piece；
The two-stage refrigeration system is made of compression refigerating machine and temperature-difference refrigerating piece.The compression refigerating machine includes condenser 12, compression
Machine 13, throttle valve 14, medium temperature refrigerant；The configuration of temperature-difference refrigerating piece 5 is made in the controllable temperature cabinet exterior and the compression
Between cold, the circulation loop of the compression refigerating machine is accessed in hot junction, and cold end is contacted with 3 outer wall of controllable temperature babinet.It is described
The heat that temperature-difference refrigerating piece 5 absorbs is transmitted to the medium temperature refrigerant, and the heat is transmitted to environment again and is situated between by the medium temperature refrigerant
Matter.
As shown in case explosive view in the cold-induced device of Fig. 2 seeds, the interior case includes heat-insulating cover 1, controllable temperature babinet 3, resists
Cold solution, the low-temperature antifreeze liquid containing MgO nano particles, ultrasonic transducer 4, spiral winding 2, stirrer 6, concentric drain cap 7
And temperature-difference refrigerating piece 5.
As shown in Fig. 3 controllable temperature box-section schematic diagrames, 3 bottom of controllable temperature babinet is configured with metal sintering multi-hole center
16, the metal sintering multi-hole center 16 is interior filled with working fluid.The one of the close low-temperature antifreeze liquid of the controllable temperature babinet 3
Side internal face is gradient surface 10, and the side internal face close to the temperature-difference refrigerating piece 5 is multiple dimensioned groove structure 9.Described
Gradient surface is the surface with gradient surface energy, and the methods of vapor deposition (CVD) may be used to prepare gradient surface energy material
Expect surface.The multiple dimensioned groove structure is specially multiple dimensioned micro-nano structure --- the wedge-shaped asymmetric groove of blind hole composition,
It can be constituted using microcavity array with different surfaces energy of high-resolution stereo lithography preparation etc..When low temperature is antifreeze
When liquid temperature increases, 5 cold end of the temperature-difference refrigerating piece caloric receptivity increases, hot junction thermal discharge increases, and the working fluid steams
Phase transformation is sent out, the working fluid of evaporation is condensed in the gradient surface 10.The metal sintering multi-hole center 16 is due to high hole
Gap rate so that heat-transfer capability is significantly better than traditional liquid-sucking core, and has excellent average temperature performance, reduces thermal resistance.The gradient
There are Surface Energy Gradients, drop will produce self-driving type under by nonequilibrium surface tension effects and be moved on surface 10,
To be excluded from the gradient surface 10 in time, condensing heat-exchange performance is can effectively ensure that and improved.The multiple dimensioned groove
Structure 9 can optimize in wall surface upwardly direction and reinforce capillary rise, and the capillary in wall surface downwardly direction is prevented to return
Stream reduces thermal resistance so that the work area of the temperature-difference refrigerating piece 5 to reach the one direction transport of liquid from bottom to up
Increase, the external heat output of low-temperature antifreeze liquid increases in the unit interval, and temperature reduces, and then achievees the purpose that control temperature.
As shown in Fig. 4 controllable temperatures babinet and evaporator connection diagram, the configuration of temperature-difference refrigerating piece 5 is in the controllable temperature
Between cabinet exterior and the evaporator 15, hot junction is connected with shown evaporator 15 by heat-conducting silicone grease, cold end with it is described can
3 outer wall of temperature control box body is connected by heat-conducting silicone grease.
As shown in Fig. 5 spiral windings control hardware block diagram, when microcontroller is responsible for the reading of button and generates corresponding electric current
Sequence control signal；Driving circuit exports corresponding electric current sequential according to the control signal of microcontroller；Spiral winding 2 is according to inflow
Electric current generates corresponding magnetic field；Power supply module is responsible for powering to microcontroller and driving chip.
As shown in Fig. 6 stirrers rotation schematic diagram, two spiral windings 2 on diagonal line are set as one group, every group of spiral
Coil 2 alives simultaneously, but current direction is on the contrary, the polarity of the magnetic field presented in synchronization is opposite.Two groups are controlled respectively
The break-make of electric current and direction in spiral winding 2, so that it may to control the direction of resultant magnetic field caused by two groups of coil different moments,
And then control the rotation of stirrer 6.
As shown in Fig. 7 ultrasonic transducer quadrangle tangential circle arrangement schematic diagrams, 11 energy converter 4 of ultrasonic wave has 4n, is divided into n
Layer, and is arranged in the surrounding of controllable temperature babinet 3 in a manner of quadrangle tangential circle by 4 every layer, wherein n ＞ 0 and n is integer.Work
When, the power supply of power supply module is converted to the corresponding high-frequency alternating current of energy converter by supersonic generator, and ultrasonic transducer 4 will be defeated
The electrical power entered is converted into mechanical output（That is ultrasonic wave 11）It passes again, four strands of ultrasonic waves 11 are at 3 center of controllable temperature babinet
An imaginary circle is formed, the low-temperature antifreeze liquid rotation containing MgO nano particles is driven, so that uniform temperature is significantly in babinet 3
It is promoted, and ultrasonic wave 11 is so that seed fully shaking in controllable temperature babinet 3, is paved completely in controllable temperature babinet 3.
Claims (10)
1. a kind of cold-induced device of seed based on ultrasonic technology, including freezing module, control module and power supply module；Institute
It includes two-stage refrigeration system and interior case to state freezing module, and the two-stage refrigeration system includes compression refigerating machine and temperature-difference refrigerating piece；
The compression refigerating machine includes evaporator, condenser, compressor and throttle valve；It is characterized in that：The interior case include heat-insulating cover,
Controllable temperature babinet, ultrasonic transducer, coil, stirrer and concentric drain cap；The ultrasonic transducer is in a manner of the circle of contact
It is arranged in the circumferential direction of the controllable temperature babinet；The coil is arranged in pairs in the controllable temperature babinet；The stirrer is located at
Controllable temperature bottom of box center；The concentric drain cap is configured with concentric assembly method in the controllable temperature bottom of box；
The temperature-difference refrigerating piece accesses the cycle of the compression refigerating machine, the steaming in temperature-difference refrigerating piece hot junction and the compression refigerating machine
Device contact is sent out, the temperature-difference refrigerating piece cold end is contacted with the controllable temperature cabinet exterior；
The control module includes microcontroller, driving circuit and supersonic generator；When the microcontroller is responsible for generating electric current
Sequence control signal；The driving circuit exports corresponding electric current sequential according to the control signal of microcontroller；The coil is according to institute
The electric current sequential for stating driving circuit output generates corresponding magnetic field；The stirrer is rotated according to different magnetic field；The ultrasonic wave
Generator is connect with the ultrasonic transducer；
The side internal face close to the low-temperature antifreeze liquid of the controllable temperature babinet is the surface with gradient surface energy, close
The side internal face of the temperature-difference refrigerating piece is multiple dimensioned groove structure, and metal sintering is configured in the controllable temperature bottom of box
Multi-hole center；
The power supply module is connected with the microcontroller, the driving circuit and the coil；The power supply module and described
Compressor connects；The power supply module is connected with the temperature-difference refrigerating piece；The power supply module and the supersonic generator, institute
State ultrasonic transducer connection.
2. the cold-induced device of seed according to claim 1, it is characterised in that：The power supply module include lithium battery and with
The charge and discharge control module for the control charge and discharge parameter that its output end is connected.
3. the cold-induced device of seed according to claim 1, it is characterised in that：The ultrasonic transducer has 4n, point
For n-layer, 4 every layer and the surrounding of the babinet is arranged in a manner of quadrangle tangential circle, the ultrasonic transducer distance is most
It is integer, L >=0 that the distance on nearly described babinet one side, which is L, wherein n ＞ 0 and n,.
4. the cold-induced device of seed according to claim 1, it is characterised in that：The concentric drain cap include N grades of radiuses not
Same concentric circles, with concentric assembly method configuration in the bottom of box, the radius of kth grade concentric circles is rk, N-1 grades same
Heart circle is tangent with the bottom of box, and wherein N >=1 and N are integer, 0≤k≤N-1.
5. the cold-induced device of seed according to claim 1, it is characterised in that：The coil is spiral winding, there is 2s,
S >=1 and s are integer.
6. the cold-induced device of seed according to claim 5, it is characterised in that：S takes 2,3,4, i.e., between the described spiral winding
Angle be respectively 90 °, 60 ° and 45 °.
7. the cold-induced device of seed according to claim 1, it is characterised in that：The material of stirrer is permanent magnet.
8. using the method according to any cold-induced device freezen protective seeds of seed of right 1-7, it is characterised in that：Including
Following steps：
Step 1: being configured to pretreated cold-resistant solution and the low-temperature antifreeze liquid containing MgO nano particles；
Step 2: the cold-resistant solution configured is placed into babinet, cold-resistant processing, cold-resistant are carried out to seed using cold-resistant solution
The time of processing is 10 ~ 15 minutes, has handled emptying cold-resistant solution；
Step 3: the low-temperature antifreeze liquid containing MgO nano particles configured is placed into babinet, freezed using the two-stage
System and controllable temperature babinet freeze, and are carried out at the same time temperature regulation and control, and the low-temperature antifreeze liquid containing MgO nano particles is tieed up
It holds at -45 DEG C~-35 DEG C；
Step 4: being stirred, being rotated using spiral winding and the stirrer, the low temperature containing MgO nano particles described in samming is prevented
Freeze liquid, the ultrasonic transducer concussion of the supersonic generator and quadrangle tangential circle arrangement rotates, contains described in samming
The low-temperature antifreeze liquid of MgO nano particles and the seed is paved, pretreatment in 30 ~ 40 minutes is carried out to the seed；
Step 5: the emptying low-temperature antifreeze liquid containing MgO nano particles is carried out by Seed storage in -18 degrees Celsius of freezers
Air-cooled preservation for a long time.
9. according to the method described in claim 8, it is characterized in that：The cold-resistant solution by mass percent following components group
At：3 ~ 5% sodium chloride, 2 ~ 8% ethyl alcohol, 1 ~ 2% glycerine, 0.2 ~ 0.8% serine, 0.1 ~ 0.5% trehalose, 0.3 ~
0.5% ribitol, 0.05 ~ 0.2% laureth sodium sulfovinate, remaining is distilled water.
10. method according to claim 8 or claim 9, it is characterised in that：The low-temperature antifreeze liquid containing MgO nano particles
Include the component of following mass percent：0.5 ~ 8% sodium chloride, 5 ~ 12% ethyl alcohol, 7 ~ 10% glycerine, 2 ~ 5% chitin,
0.5 ~ 1.5% alanine, 0.1 ~ 0.5% antierythrite, 0.05 ~ 0.3% American cockroach drying polypide ethanol extract,
0.1 ~ 5% MgO nano particles, remaining is distilled water.
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| CN201810647856.6A CN108522500B (en) | 2018-06-22 | 2018-06-22 | A kind of seed cold-induction device and method based on ultrasonic technology |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110475465A (en) * | 2019-09-18 | 2019-11-19 | 山东大学 | A kind of hot-pipe system of jet hole height change |
| CN110572992A (en) * | 2019-09-18 | 2019-12-13 | 山东大学 | Immersed self-turbulent flow cooling system with four-corner tangential circles |
| CN110631301A (en) * | 2019-10-21 | 2019-12-31 | 山东大学 | Immersed self-turbulent-flow shell-and-tube heat exchanger |
| CN111373900A (en) * | 2020-03-16 | 2020-07-07 | 韩永苗 | Breeding method of cyperus esculentus by cold induction technology |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1030194A (en) * | 1988-05-18 | 1989-01-11 | 化学工业部炭黑工业研究设计所 | Ultrasonic electromagnetic separating apparatus |
| CN106258070A (en) * | 2016-08-10 | 2017-01-04 | 国俊伍 | A kind of seed freezing storage device and method |
| CN107551920A (en) * | 2016-07-01 | 2018-01-09 | 中国科学院声学研究所 | A kind of multiple dimensioned mixing machine and mixing method |
| CN207271177U (en) * | 2017-09-11 | 2018-04-27 | 官爱平 | Ultrasonic wave agitating device |
-
2018
- 2018-06-22 CN CN201810647856.6A patent/CN108522500B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1030194A (en) * | 1988-05-18 | 1989-01-11 | 化学工业部炭黑工业研究设计所 | Ultrasonic electromagnetic separating apparatus |
| CN107551920A (en) * | 2016-07-01 | 2018-01-09 | 中国科学院声学研究所 | A kind of multiple dimensioned mixing machine and mixing method |
| CN106258070A (en) * | 2016-08-10 | 2017-01-04 | 国俊伍 | A kind of seed freezing storage device and method |
| CN207271177U (en) * | 2017-09-11 | 2018-04-27 | 官爱平 | Ultrasonic wave agitating device |
Non-Patent Citations (1)
| Title |
|---|
| 万书晓: "《机动车发动机冷却液》", 30 September 2014, 中国石化出版社 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110475465A (en) * | 2019-09-18 | 2019-11-19 | 山东大学 | A kind of hot-pipe system of jet hole height change |
| CN110572992A (en) * | 2019-09-18 | 2019-12-13 | 山东大学 | Immersed self-turbulent flow cooling system with four-corner tangential circles |
| CN110475465B (en) * | 2019-09-18 | 2020-05-22 | 山东大学 | Heat pipe system with jet hole height changing |
| CN110631301A (en) * | 2019-10-21 | 2019-12-31 | 山东大学 | Immersed self-turbulent-flow shell-and-tube heat exchanger |
| CN110631301B (en) * | 2019-10-21 | 2021-06-08 | 山东大学 | Immersed self-turbulent-flow shell-and-tube heat exchanger |
| CN111373900A (en) * | 2020-03-16 | 2020-07-07 | 韩永苗 | Breeding method of cyperus esculentus by cold induction technology |
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