CN108514613A - Chinese medicine composition and its preparation method and application with antitumaous effect - Google Patents

Chinese medicine composition and its preparation method and application with antitumaous effect Download PDF

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CN108514613A
CN108514613A CN201810654420.XA CN201810654420A CN108514613A CN 108514613 A CN108514613 A CN 108514613A CN 201810654420 A CN201810654420 A CN 201810654420A CN 108514613 A CN108514613 A CN 108514613A
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chinese medicine
medicine composition
antitumaous effect
root
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金志华
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    • A—HUMAN NECESSITIES
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
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    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/42—Cucurbitaceae (Cucumber family)
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    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
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    • A61K36/185—Magnoliopsida (dicotyledons)
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    • A61K2236/35—Extraction with lipophilic solvents, e.g. Hexane or petrol ether

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Abstract

The invention discloses a kind of Chinese medicine composition and its preparation method and application with antitumaous effect, the Chinese medicine composition with antitumaous effect includes following raw materials：Radix Salviae Miltiorrhizae, Chinese yam, Rhizoma Atractylodis Macrocephalae, the bletilla striata, black nightshade, Ma Jiaoer, rhizome of chuanxiong, rheum officinale, the root of bidentate achyranthes, Paris polyphylla, reed root, cordate houttuynia.The Chinese medicine composition with antitumaous effect of the present invention, it then follows Chinese medicine combination principle can reduce cancer cell vigor, improve immunity of organisms, enhance the resistance to cancer cell, treating both manifestation and root cause of disease, phase cancer symptoms have good efficacy in the morning, afternoon and evening to prostate cancer, liver cancer, gastric cancer, lung cancer, cancer of the esophagus etc..

Description

Chinese medicine composition and its preparation method and application with antitumaous effect
Technical field
The present invention relates to a kind of Chinese medicine composition and its preparation method and application with antitumaous effect.
Background technology
Cancer is a kind of malignant tumour.More than annual about 2,500,000 people of new cancer patient in China, controlling patient 600 every year More than ten thousand people, the number for dying of cancer every year is more than 1,400,000 people, and growth rate is surprising, and average per dead 5 people, just there are about 1 People is to die of cancer.Normal cell is prominent due to proto-oncogene caused by the carcinogens such as physics, chemistry, virus and tumor suppressor gene Become and is changed into cancer cell.Cancer cell is a kind of cell of variation, is the pathogeny for generating cancer, it is big that cancer cell is broadly divided into three Class：Squamous carcinoma, gland cancer, undifferentiated carcinoma.Cancer cell is different from normal cell, has infinite multiplication, can convert and easily shift three big features, Infinite multiplication and normal cell tissue can be destroyed.Cancer cell in addition to divide it is out of control other than, can also locally invade arround normal group It knits and is even transferred to body other parts via body-internal-circulation system or lymphatic system.In general, cancer cell carries out transfer meeting It is divided into several stages：First stage is known as invading, and cancer epithelial cell can unclamp the connection between cancer cell in this stage, make It obtains cancer cell " recapturing freedom " and can be moved to and go elsewhere.Second stage oozes in being known as, and cancer cell passes through blood vessel or leaching The endothelium of hand shaft enters the circulatory system.Phase III is known as exosmosing, in this stage, what the trip by the circulatory system baptized Survivor can pass through the endothelial cell of capilary to reach other tissues.The last stage is exactly the New World of these cancer cells Immigrant multiplies the healthy and strong malignant tumour for forming transfer in its hetero-organization.Compared with benign tumour, malignant growth speed Soon, be in infiltrative growth, bleeding, necrosis, ulcer etc. easily occur, and often have DISTANT METASTASES IN, cause human body to become thin, inability, anaemia, Loss of appetite, fever and serious organ function are impaired etc., ultimately cause death.
Existing cancer treatment method is largely with means such as operation, radiotherapy, chemotherapy, Oral Chemical drugs, these are controlled Treatment mode can also kill normal cell, patient is caused to bear excessive pain because of over-treatment while killing cancer cell Or it is dead.Also some patients due to apocleisis or cannot feed and be unable to oral drugs, cause to lose and control and dead.Cancer is the world The great difficult problem of various concerns, it would be highly desirable to there is the significant drug of new curative effect to occur.Traditional Chinese medicine earlier than before two thousand years to cancer Recognized, tcm therapy then has its unique theoretical logic and the mode of thinking.Chinese medicine can effectively inhibit tumour cell, and match Chemicotherapy is closed to adjust immunity of organism, induction tumor cell differentiation and apoptosis, prevent metastasis of cancer recurrence and inhibit the works such as precancerous lesion Achieved the purpose that prevent cancer with mechanism.The research of Chinese medicine antitumor mechanism is just from cellular level to molecular level, gene Level carries out depth research from single component to use in conjunction.Chinese medicine antitumor drug research and development under instruction of Chinese Medicine theory, knot The development of modern medicine is closed, extraction process greatly promotes, and becomes the hot spot studied both at home and abroad in recent years.Advantages of TCM is to alleviate disease Shape it is with obvious effects, in carcinosis radiotherapy and chemotherapy it is secondary it is iatrogenic damage have good auxiliary therapeutic action, can be directed to disease Original treatment.Chinese medicine also has sensitization to chemicals, using eliminating pathogen to support vital qi, the Chinese traditional treatment principle strengthened the body resistance to consolidate the constitution, with chemotherapy Cooperation can heighten the effect of a treatment, and mitigate the adverse reaction of chemotherapeutics.Simultaneously, moreover it is possible to help patient to improve immunity, improve blood Index, and then change its malaise symptoms, improve the life quality of tumor patient.
Invention content
The present invention provides a kind of Chinese medicine composition and its preparation method and application with antitumaous effect.The present invention uses Following technical solution：
A kind of Chinese medicine composition with antitumaous effect, including following raw materials：Radix Salviae Miltiorrhizae, Chinese yam, Rhizoma Atractylodis Macrocephalae, the bletilla striata, black nightshade, horse Hand over youngster, rhizome of chuanxiong, rheum officinale, the root of bidentate achyranthes, Paris polyphylla, reed root, cordate houttuynia.
A kind of Chinese medicine composition with antitumaous effect, includes the raw material of following weight parts：40-80 parts of Radix Salviae Miltiorrhizae, Chinese yam 15- 30 parts, 10-30 parts of Rhizoma Atractylodis Macrocephalae, 30-60 parts of the bletilla striata, 30-60 parts of black nightshade, horse hand over 10-40 parts of youngster, 15-45 parts of rhizome of chuanxiong, rheum officinale 5-10 Part, 10-30 parts of the root of bidentate achyranthes, 3-10 parts of Paris polyphylla, 3-10 parts of reed root, 5-15 parts of cordate houttuynia.
A kind of Chinese medicine composition with antitumaous effect, includes the raw material of following weight parts：40-80 parts of Radix Salviae Miltiorrhizae, Chinese yam 15- 30 parts, 10-30 parts of Rhizoma Atractylodis Macrocephalae, 30-60 parts of the bletilla striata, 30-60 parts of black nightshade, horse hand over 10-40 parts of youngster, 15-45 parts of rhizome of chuanxiong, rheum officinale 5-10 Part, 10-30 parts of the root of bidentate achyranthes, 3-10 parts of Paris polyphylla, 3-10 parts of reed root, 5-15 parts of cordate houttuynia, 15-45 parts of complex polysaccharide.
A kind of Chinese medicine composition with antitumaous effect, includes the raw material of following weight parts：40-80 parts of Radix Salviae Miltiorrhizae, Chinese yam 15- 30 parts, 10-30 parts of Rhizoma Atractylodis Macrocephalae, 30-60 parts of the bletilla striata, 30-60 parts of black nightshade, horse hand over 10-40 parts of youngster, 15-45 parts of rhizome of chuanxiong, rheum officinale 5-10 Part, 10-30 parts of the root of bidentate achyranthes, 3-10 parts of Paris polyphylla, 3-10 parts of reed root, 5-15 parts of cordate houttuynia, 15-45 parts of complex polysaccharide, quinoa white fungus powder 40-70 parts.
A kind of Chinese medicine composition with antitumaous effect, includes the raw material of following weight parts：40-80 parts of Radix Salviae Miltiorrhizae, Chinese yam 15- 30 parts, 10-30 parts of Rhizoma Atractylodis Macrocephalae, 30-60 parts of the bletilla striata, 30-60 parts of black nightshade, horse hand over 10-40 parts of youngster, 15-45 parts of rhizome of chuanxiong, rheum officinale 5-10 Part, 10-30 parts of the root of bidentate achyranthes, 3-10 parts of Paris polyphylla, 3-10 parts of reed root, 5-15 parts of cordate houttuynia, 15-45 parts of complex polysaccharide, quinoa white fungus powder 40-70 parts, 10-40 parts of passionflower fruit powder, 10-40 parts of white bur powder.
The preparation method of complex polysaccharide described in the embodiment of the present invention is as follows：Agricus blazei, radix polygonati officinalis, moringa seeds are crushed respectively 10-20 mesh sieve is crossed afterwards, is (1-3) in mass ratio by Agricus blazei, radix polygonati officinalis, the moringa seeds after crushing：(1-3)：(1-3) is mixed to get 1000-2000g ether is added into 400-800g mixtures for mixture, is 30-100 revs/min of stirring in 30-35 DEG C, rotating speed 3-15 minutes, 50-100 mesh sieve is crossed, first-time filtrate and a filter cake are obtained, by a filter cake at 40-60 DEG C of dry 20-45 points Clock a filter cake after drying is added in 3-8L water, frequency is 20-40KHz, power is 300-500W condition ultrasonic extractions 40-90 minutes, 200-500 mesh sieve is crossed, secondary filtrate and secondary filter cake is obtained, secondary filtrate is concentrated by evaporation extremely at 50-75 DEG C The 30-65% of original volume obtains concentrate, and ethyl alcohol is added into concentrate, and the volume fraction of ethyl alcohol is made to reach 65-90%, mixing 12-36 hours are stood after uniformly, is centrifuged 10-30 minutes with 3000-8000 revs/min of rotating speed, is deposited in what centrifugation obtained 0.04-0.08MPa, 40-60 DEG C 6-24 hours dry, obtains complex polysaccharide.Polysaccharide has antibacterial, anti-inflammatory, analgesic, decompression, resists Tumour, enhancing body immunity, antifatigue effect.Above-mentioned effect can be preferably realized by preferred polysaccharide source and proportioning Fruit.Preferably, it is 1 in mass ratio by Agricus blazei, radix polygonati officinalis, the moringa seeds after crushing：3：1 is mixed to get mixture.
The preparation method of the powder of quinoa white fungus described in the embodiment of the present invention is as follows：White fungus is dried at 45-60 DEG C to aqueous Amount is 10-20%, is crushed, and crosses 10-20 mesh sieve, obtains white fungus powder, after 200-500g quinoas, 200-800g water are mixed, heating To 75-90 DEG C, 20-60 minutes are kept the temperature at 75-90 DEG C, is cooled to 35-40 DEG C, 100-300g white fungus powder, 0.5-5g lemons is added Acid, 10-50g lactobacillus plantarums seal after mixing, and keeping the temperature 24-72 hours at 35-40 DEG C ferments, by the production after fermentation Object is 12-36 hours dry at 50-70 DEG C, and 5-15s is sterilized at 110-125 DEG C, crushes, and crosses 15-20 mesh sieve, obtains quinoa white fungus Powder.Preferably, the fermentation is to keep the temperature 48 hours at 35-40 DEG C to ferment.Quinoa has enhancing body function, dummy Matter adjusts immune and endocrine, raising physical stress ability, prevention disease, anticancer and other effects.It is anti-that white fungus can not only enhance body The immunocompetence of tumour, moreover it is possible to enhance tolerance of the tumor patient to radiotherapy, chemotherapy.Quinoa white fungus can improve medicine by fermentation Effect is accelerated to absorb, improves taste, and structural modification can also be carried out to functional component by fermentation, in addition obtain it is new it is effective at Point.
The preparation method of passionflower fruit powder described in the embodiment of the present invention is as follows：Passionfruit is removed the peel, after peeling 200-500g passionfruits are 1 by solid-liquid ratio with water:It is homogenized after (0.5-2) g/mL mixing, passionflower pulp is obtained, to passionflower 0.05-0.5g cellulases, 0.05-0.5g pectases are added in pulp, is warming up to 35-40 DEG C, it is small that 2-6 is kept the temperature at 35-40 DEG C When, it is warming up to 85-95 DEG C, 10-20 minutes are kept the temperature at 85-95 DEG C, is cooled to 35-40 DEG C, the mixing of 50-150g maltodextrins is added Uniformly, spray drying obtains the passionflower fruit powder that water content is 5-15wt%；The preparation method of the white bur powder is as follows：It will The white burs of 200-500g are 1 by solid-liquid ratio with water:It is homogenized after (0.5-2) g/mL mixing, white bur slurry is obtained, in being starched to white bur 3-20g citric acids, 0.1-1g sodium chloride, 0.05-0.5g pectases is added, is warming up to 35-40 DEG C, it is small that 2-6 is kept the temperature at 35-40 DEG C When, it is warming up to 85-95 DEG C, 5-15 minutes are kept the temperature at 85-95 DEG C, is cooled to 35-40 DEG C, the mixing of 50-150g maltodextrins is added Uniformly, spray drying obtains the white bur powder that water content is 5-15wt%.When preparing fruit powder, the rotating speed of the homogenate is 15000-20000 turns/min, Homogenization time 3-15min.The inlet air temperature being spray-dried when preparing fruit powder：140-165 DEG C, Leaving air temp is 65-90 DEG C.
The present invention also provides the preparation methods of the Chinese medicine composition with antitumaous effect：Each raw material is distinguished into powder Broken, 30-50 mesh excessively sieves, and the raw material after crushing is uniformly mixed to obtain the final product.
The present invention also provides a kind of use of Chinese medicine composition with antitumaous effect in preparing antitumor, anticancer drug On the way.The present invention also provides a kind of purposes of Chinese medicine composition with antitumaous effect in the drug for preparing anti-prostate cancer.It will There is the present invention Chinese medicine composition of antitumaous effect to be mixed with the available auxiliary material of medicine, pelletize according to common process to obtain the final product.
The Chinese medicine composition with antitumaous effect of the present invention, it then follows Chinese medicine combination principle can reduce cancer cell vigor, carry High immunity of organisms enhances the resistance to cancer cell, treating both manifestation and root cause of disease, to prostate cancer, liver cancer, gastric cancer, lung cancer, cancer of the esophagus etc. Phase cancer symptoms have good efficacy in the morning, afternoon and evening.
On the basis of common knowledge of the art, above-mentioned each optimum condition, can be each preferably to get the present invention in any combination Embodiment.
Specific implementation mode
It is further illustrated the present invention below by the mode of embodiment, but does not therefore limit the present invention to the reality It applies among a range.In the following examples, the experimental methods for specific conditions are not specified, according to conventional methods and conditions, or according to quotient Product specification selects.
In following embodiments, primary raw material and instrument used are as follows：
Radix Salviae Miltiorrhizae, the dry root of labiate Radix Salviae Miltiorrhizae Salvia miltiorrhiza Bge. have promoting blood circulation, promoting menstruation The effect of analgesic, relieving restlessness that clears away heart-fire, cool blood to disappear carbuncle.Place of production Hebei.
Chinese yam, the dry rhizome of Dioscoreaceae plant Chinese yam Dioscorea opposita Thunb., modern pharmacology experiment knot Fruit shows that Chinese yam has promotion union, hypoglycemic, the effects that enhancing immune function, enhance digestive system function.Place of production river Soviet Union.
Rhizoma Atractylodis Macrocephalae, the dry rhizome of feverfew Rhizoma Atractylodis Macrocephalae Atractylodes macrocephala Koidz. have invigorating the spleen The effect of QI invigorating, eliminate dampness and have diuretic effect, hidroschesis, tocolysis, is used for spleen eating less, and abdominal distention diarrhea, phlegm retention anti-dazzle nervous, oedema, spontaneous perspiration, movement of the foetus is not Peace.Place of production Jiangsu.
The bletilla striata, the dry tuber of orchid bletilla Bletilla striata (Thunb.) Reichb.f., can restrain and stop Blood, detumescence and promoting granulation.It spits blood for hemoptysis, traumatic hemorrhage, sore swollen toxin, chapped skin；Pulmonary tuberculosis hemoptysis, ulcer haemorrhage.Production Ground Jiangsu.
Black nightshade is the drying herb of Solanaceae nightshade black nightshade Solanum nigrum L..For clearing heat and detoxicating, Li Shui Detumescence.For cat fever, toothache, chronic bronchitis, dysentery, urinary infection, mastitis, leukorrhea, cancer.Pacify in the place of production Emblem.
Horse hands over youngster, hands over youngster platymiscium horse to hand over youngster Melothria ndica Lour. [Zehneria for Curcurbitaceae horse Indica (Lour.) C.Y.Wu] dry root.It can clearing heat and detoxicating, dispersing swelling and dissipating binds.For abscess of throat, conjunctivitis；Sore is controlled in external application Ulcer pyogenic infections, scrofula, orchitis, skin eczema.Place of production Jiangsu.
Rhizome of chuanxiong is the dry rhizome of samphire Rhizoma Chuanxiong Ligusticum chuanxiong Hort., acrid in flavour and warm in nature.Return Liver, courage, pericardium channel, blood-activating and qi-promoting, wind-expelling pain-stopping.Place of production Sichuan.
Rheum officinale uses the dry root of polygonum rheum palmatum Rheum palmatum L, major function：Purge heat logical intestines, Removing pattogenic heat from the blood and toxic material from the body, dispelling stasis of blood and stimulating the menstrual flow.For real heat constipation, stagnate abdominal pain, and rush down dysentery is not well, jaundice with damp-heat pathogen, blood-head tells nosebleed, hot eyes, pharynx swelling, Abdominalgia with intestinal abscess, carbuncle swells furunculosis, blood stasis closed, traumatic injury control scald outside.Place of production Gansu.
The root of bidentate achyranthes is the dry root of amaranthaceous plant root of bidentate achyranthes Achyranthes bidentata Blume., major function：Tonifying liver Kidney, strengthening the bones and muscles, dispelling stasis of blood and stimulating the menstrual flow are ensuringd proper downward flow of the blood.Place of production Hebei.
Paris polyphylla is the dry rhizome of liliaceous plant Yunnan Rhizoma Paridis Paris yunnanensis Franch..Function master It controls：It is clearing heat and detoxicating, swelling and pain relieving, cool liver arresting convulsion.Make in malignant boil swell carbuncle swells, abscess of throat, venomous snake bite, the pain of injury caused by falling and tumbling, infantile convulsion take out It jerks.Place of production Yunnan.
Reed root is the dry rhizome of grass reed Phragmites communis Trin..Major function：Clearly Heat is promoted the production of body fluid, relieving restlessness, preventing or arresting vomiting, diuresis.For pyreticosis polydipsia, stomach hot vomiting, cough with lung heat, lung carbuncle pyemesis, puckery pain of heat gonorrhea.Place of production mountain East.
Cordate houttuynia, Classification system：Houttuynia cordata Thunb. are the dry ground of saururaceae plant houttuynia cordata Upper part.Energy is clearing heat and detoxicating, sore, promoting urination and removing dampness, clearing away heat to cure dysentery, stomach strengthening and digestion promoting are treated in detumescence, hot with real heat, heat toxin, damp evil, disease is controlled For the lung carbuncle of trouble, sore swollen toxin, hemorrhoidal hemorrhage, retention of heat in the spleen and stomach etc..Modern pharmacology experiment show this product have antibacterial, it is antiviral, The effects that improving immunity of organisms, diuresis.Place of production Shaanxi.
Agricus blazei, Classification system：Agaricus blazei Murill, Agricus blazei is good healthy food, to high blood Pressure, hyperlipidemia, refining system disease, diabetes, breast cancer, oophoroma decreased energy, influenza etc. have certain treatment Effect.Agricus blazei is made of mycelium and fructification two parts, and the fructification of Agricus blazei, place of production Jilin are used in the present invention.
Radix polygonati officinalis, Classification system：Polygonatum odoratum (Mill.) Druce plants for Liliaceae perennial herb Object.Mainly contain the ingredients such as fragrant solomonseal rhizome polyoses, steroid saponin, flavones, tannin, sterol, mucilaginous substance, alkaloid and cardiac glycoside.This hair It is bright to use fresh polygonati rhizoma, radix polygonati officinalis place of production Heilungkiang.
Moringa seeds, the seed of Moringa Moringa oleifera.Moringa seeds are reducing blood fat, blood pressure, weight-reducing, adjusting Stomach, protect liver abstinence from alcohol, enhancing human immunity aspect has significant curative effect.Place of production Yunnan.
White fungus is the fructification of door Basidiomycota fungi white fungus, and white fungus is by the pancake valve of the thin and more gauffer of more than 10 pieces Piece forms.Tremella fructification is pure white to milky, and generally in chrysanthemum shape or cockscomb shape, 5~10 centimetres of diameter is soft pure white, and half Transparent, high resilience is similar to cosmos, tree peony shape or silk ball shape, 3~15 centimetres of diameter by several pieces to forming.White fungus has strong Essence, kidney tonifying, ease constipation, beneficial stomach, tonifying Qi and blood, cardiac stimulant, strong body, cerebrum tonifying, refresh oneself, beauty, tender skin, the effect of promoting longevity.Its energy Liver detoxification ability is improved, protects liver function, it can not only enhance the antitumor immunocompetence of body, moreover it is possible to enhance tumour trouble Tolerance of the person to radiotherapy, chemotherapy.It can nourish and promote the production of body fluid；Moistening lung nourishing the stomach, invigorating the lung and benefiting vital QI.Cough due to consumptive disease；Blood-stained sputum；Tianjin Shao Kou Yearningly；It is physically weak after being ill；Shortness of breath and fatigue.Place of production Sichuan.
Quinoa, Classification system：Chenopodium quinoa Willd.Quinoa is a kind of grain, originates in South America peace This mountain area.Quinoa is circular tablets shape, and volume to weight ratio millet is slightly light, general mass of 1000 kernel 3-4g.The quinoa production used in the present invention Ground Shaanxi.Quinoa belongs to easy ripe food easy to digest, unique in taste, has light nut faint scent or ginseng fragrant, has balanced mend Fill nutrition, enhancing body function, repair constitution, adjust immune and endocrine, improve physical stress ability, prevent disease, anticancer, Weight-reducing, auxiliary treatment and other effects.
Lactobacillus plantarum, Classification system：Lactobacillus plantarum, by the limited public affairs of Xi'an rhythm standing grain biotechnology Department provides, bacterial strain Han Liang≤10,000,000,000 cfu/g, model YH-626.
Passionfruit, passionflower (scientific name：Passiflora caerulea L.) it is that perennial evergreen climbs woody climber Plant, passionfruit are a kind of fruit that fragrance is palatable, there is the good reputation of " king of fruit juice ".Contain abundant albumen in passionfruit Up to 165 kinds of compounds such as matter, fat, reduced sugar, multivitamin and phosphorus, calcium, iron, potassium and needed by human 17 kinds of amino Acid, nutritive value is very high, wherein vitamin content 1345 (1U/100G), is the effective ingredient of anticancer, 1.7 milli of Yan acid contents Gram/100G, it is positive for preventing angiocardiopathy from playing the role of.Place of production Guangxi.
White bur, the white bur kind that the present invention uses are Nitraria tangutorum fruit, place of production Qinghai.White thorn is puncture vine actions and spoken parts in classical Chinese drama thorn The shrub of category, berry of nitraria tangutorum bobr size such as big beans are in oval, are in dark red or orange-yellow after ripe.White bur sweet and sour It is full of nutrition, it has developed and plurality kinds of health care, nutrition series of products, such as feed, fruit wine, fruit juice and jam has been made.
Cellulase, using trichoderma reesei (Classification system：Trichodermareesei) through liquid deep layer fermenting, ultrafiltration Etc. processes it is refined made of highly effective cellulose enzyme preparation.Enzyme activity：7000u/g is carried by Ningxia Sunson Industrial Group Co., Ltd. For model full constituent type 7000.
Pectase, enzyme activity 10,000 U/g, model SAC-015 are purchased from Ningxia Sunson Industrial Group Co., Ltd..
Maltodextrin meets GB/T 20884-2007 standards, 11≤DE values<16, it is purchased from the western limited public affairs of king's sugar industry in Shandong Department.
It is homogenized equipment：The visual high-speed tissue mashing machine of JJ-2B glass of the lofty and steep Instrument Ltd.'s production in Changzhou.
Test method
The Chinese medicine composition 100g with antitumaous effect of the present invention is added in 800mL water, is impregnated 2 hours at 35 DEG C, 75 DEG C are warming up to, 25 minutes is kept the temperature at 75 DEG C, sieves with 100 mesh sieve, filtrate is concentrated into 200mL in 95 DEG C of heating, obtains concoction.
Cancer cell vigor is tested
Experiment material：Castration-resistant prostate cancer cell strain PC3 is provided by Sun Yat-sen Memorial Hospital, with containing The F-12K culture mediums of 10% peptide cow's serum are cultivated.MTT (cell Proliferation and cytotoxicity) detection kit is purchased from the U.S. Sigma-Aldrich companies.
Cancer cell viability experiment：The PC3 cells of logarithmic growth phase are made cell suspension inoculation in 96 orifice plates, are connect per hole 190 μ L of kind, cell density are 5 × 103A/hole, wait for that cell is adherent makes the concentration of concoction reach 100 μ g/ to every hole addition concoction ML continues culture 24,48,72h, continues to cultivate 4h to every hole 10 μ LMTT solution of addition after cultivating the above-mentioned time, measures 570nm Locate absorbance (A) value.Cell viability (Cell viability, %)=(dosing juice group A values/blank group A values) × 100%.Point Concoction is not prepared with the Chinese medicine composition with antitumaous effect of embodiment 1-8, comparative example 1-2, carries out cancer cell viability experiment, Experimental result takes the average value of 5 parallel laboratory tests.
Strengthen immunity is tested
Modeling：Experimental animal be SD rats, 25 ± 3g of weight, totally 120, half male and half female.120 mouse are randomly divided into 12 groups, each 5 of every group of male and female, respectively experimental group 1-10 groups, Normal group, model group.Normal group and model group fill Stomach physiological saline, experimental group 1-10 groups distinguish the Chinese medicine composition with antitumaous effect of gavage embodiment 1-8, comparative example 1-2 The concoction of preparation, given low 2g/Kg, once a day, continuous gavage three weeks.It is remaining 11 groups small in addition to Normal group It is lowly small to obtain CTX immunogenicity for three days on end by mouse the 15th day intraperitoneal injection of cyclophosphamide (CTX) 80mg/kg after gavage Mouse model.
Thymus gland and index and spleen index detection：Last dose for 24 hours after, weigh, put to death mouse, weigh thymus gland and spleen.It presses respectively Following formula calculates Thymus and Spleen index：
Thymus index (mg/g)=thymic factor D injection (mg)/weight (g), index and spleen index (mg/g)=spleen weight (mg)/body Weight (g).
Ability of macrophage eating detects：Last dose for 24 hours after, weigh, tail vein injection prepared Chinese ink, after injection respectively at 2,10min vena ophthalmica takes 20 μ L of blood, is added in 0.1% sodium carbonate liquors of 2mL, mixing, and absorbance A is measured at 600nm.End It is secondary take blood after, put to death mouse immediately, weigh the quality of liver and spleen, be calculated as follows clean up index K and phagocytic index respectively α。
K=(lgA1- lgA2)/(t2- t1)
α=weight/(spleen weight+liver weight) × K1/3
Wherein, A1、A2Respectively 2, the absorbance of 10min counter samples；t1、t2Respectively 2,10min.
It is further illustrated the present invention below by the mode of embodiment.
Embodiment 1
A kind of Chinese medicine composition with antitumaous effect, includes the raw material of following weight parts：60 parts of Radix Salviae Miltiorrhizae, 25 parts of Chinese yam, 20 parts of Rhizoma Atractylodis Macrocephalae, 45 parts of the bletilla striata, 35 parts of black nightshade, horse hand over 30 parts of youngster, 35 parts of rhizome of chuanxiong, 8 parts of rheum officinale, 20 parts of the root of bidentate achyranthes, 5 parts of Paris polyphylla, reed root 8 Part, 10 parts of cordate houttuynia.
The preparation method of Chinese medicine composition with antitumaous effect：Each raw material is crushed respectively, crosses 30 mesh sieve, after crushing Raw material be uniformly mixed to obtain the final product.
Embodiment 2
A kind of Chinese medicine composition with antitumaous effect, includes the raw material of following weight parts：60 parts of Radix Salviae Miltiorrhizae, 25 parts of Chinese yam, 20 parts of Rhizoma Atractylodis Macrocephalae, 45 parts of the bletilla striata, 35 parts of black nightshade, horse hand over 30 parts of youngster, 35 parts of rhizome of chuanxiong, 8 parts of rheum officinale, 20 parts of the root of bidentate achyranthes, 5 parts of Paris polyphylla, reed root 8 Part, 10 parts of cordate houttuynia, 25 parts of complex polysaccharide.
The preparation method of complex polysaccharide is as follows：Agricus blazei, radix polygonati officinalis, moringa seeds are smashed it through into 10 mesh sieve respectively, will be crushed Agricus blazei, radix polygonati officinalis, moringa seeds afterwards are 1 in mass ratio：3：1 is mixed to get mixture, and 1500g second is added into 500g mixtures Ether is 50 revs/min in 30 DEG C, rotating speed and stirs 5 minutes, crosses 50 mesh sieve, first-time filtrate and a filter cake are obtained, by a filter cake Dry 20 minutes at 40 DEG C, a filter cake after drying is added in 5L water, frequency is 20KHz, power is that 300W conditions are super Sound extracts 40 minutes, crosses 200 mesh sieve, obtains secondary filtrate and secondary filter cake, secondary filtrate is concentrated by evaporation at 50 DEG C to substance Long-pending 30% obtains concentrate, and ethyl alcohol is added into concentrate, so that the volume fraction of ethyl alcohol is reached 75%, stands after mixing It 24 hours, is centrifuged 15 minutes with 5000 revs/min of rotating speeds, is deposited in 0.06MPa, 40 DEG C of dryings 8 hours by what centrifugation obtained, obtains To complex polysaccharide.
The preparation method of Chinese medicine composition with antitumaous effect：Each raw material is crushed respectively, crosses 30 mesh sieve, after crushing Raw material be uniformly mixed to obtain the final product.
Embodiment 3
It is substantially the same manner as Example 2, it differs only in, in the present embodiment 3, the preparation method of complex polysaccharide is as follows：It will Agricus blazei, radix polygonati officinalis, moringa seeds smash it through 10 mesh sieve respectively, are 3 in mass ratio by Agricus blazei, radix polygonati officinalis, the moringa seeds after crushing： 1：1 is mixed to get mixture, and 1500g ether is added into 500g mixtures, and being 50 revs/min in 30 DEG C, rotating speed stirs 5 points Clock crosses 50 mesh sieve, obtains first-time filtrate and a filter cake, and a filter cake is 20 minutes dry at 40 DEG C, will be primary after drying Filter cake is added in 5L water, and frequency is 20KHz, power is 300W conditions ultrasonic extraction 40 minutes, the sieve of 200 mesh excessively obtains secondary Secondary filtrate is concentrated by evaporation at 50 DEG C to the 30% of original volume and obtains concentrate by filtrate and secondary filter cake, adds into concentrate Enter ethyl alcohol, the volume fraction of ethyl alcohol is made to reach 75%, stand 24 hours after mixing, 15 are centrifuged with 5000 revs/min of rotating speeds Minute, it is deposited in 0.06MPa, 40 DEG C of dryings 8 hours by what centrifugation obtained, obtains complex polysaccharide.
Embodiment 4
It is substantially the same manner as Example 2, it differs only in, in the present embodiment 4, the preparation method of complex polysaccharide is as follows：It will Agricus blazei, radix polygonati officinalis, moringa seeds smash it through 10 mesh sieve respectively, are 1 in mass ratio by Agricus blazei, radix polygonati officinalis, the moringa seeds after crushing： 1：3 are mixed to get mixture, and 1500g ether is added into 500g mixtures, and being 50 revs/min in 30 DEG C, rotating speed stirs 5 points Clock crosses 50 mesh sieve, obtains first-time filtrate and a filter cake, and a filter cake is 20 minutes dry at 40 DEG C, will be primary after drying Filter cake is added in 5L water, and frequency is 20KHz, power is 300W conditions ultrasonic extraction 40 minutes, the sieve of 200 mesh excessively obtains secondary Secondary filtrate is concentrated by evaporation at 50 DEG C to the 30% of original volume and obtains concentrate by filtrate and secondary filter cake, adds into concentrate Enter ethyl alcohol, the volume fraction of ethyl alcohol is made to reach 75%, stand 24 hours after mixing, 15 are centrifuged with 5000 revs/min of rotating speeds Minute, it is deposited in 0.06MPa, 40 DEG C of dryings 8 hours by what centrifugation obtained, obtains complex polysaccharide.
Embodiment 5
A kind of Chinese medicine composition with antitumaous effect, includes the raw material of following weight parts：60 parts of Radix Salviae Miltiorrhizae, 25 parts of Chinese yam, 20 parts of Rhizoma Atractylodis Macrocephalae, 45 parts of the bletilla striata, 35 parts of black nightshade, horse hand over 30 parts of youngster, 35 parts of rhizome of chuanxiong, 8 parts of rheum officinale, 20 parts of the root of bidentate achyranthes, 5 parts of Paris polyphylla, reed root 8 Part, 10 parts of cordate houttuynia, 25 parts of complex polysaccharide, 55 parts of quinoa white fungus powder.
The preparation method of complex polysaccharide is the same as embodiment 2.
The preparation method of the quinoa white fungus powder is as follows：It is 15% that white fungus, which is dried at 50 DEG C to water content, crushes, crosses 10 Mesh sieves, and obtains white fungus powder, after 300g quinoas, 300g water are mixed, is warming up to 85 DEG C, keeps the temperature 40 minutes at 85 DEG C, be cooled to 35 DEG C, 200g white fungus powder, 1.5g citric acids, 35g lactobacillus plantarums is added and seals after mixing, 48 hours are kept the temperature at 35 DEG C and is carried out Fermentation, the product after fermentation is 24 hours dry at 50 DEG C, 10s is sterilized at 120 DEG C, is crushed, and is crossed 15 mesh sieve, is obtained quinoa white fungus Powder.
The preparation method of Chinese medicine composition with antitumaous effect：Each raw material is crushed respectively, crosses 30 mesh sieve, after crushing Raw material be uniformly mixed to obtain the final product.
Embodiment 6
It is substantially the same manner as Example 5, it differs only in, in the present embodiment 6, the preparation method of quinoa white fungus powder is as follows： It is 15% that white fungus, which is dried at 50 DEG C to water content, is crushed, and crosses 10 mesh sieve, obtains white fungus powder, and 300g quinoas, 300g water are mixed Afterwards, 85 DEG C are warming up to, 40 minutes are kept the temperature at 85 DEG C, is cooled to 35 DEG C, 200g white fungus powder, 1.5g citric acids, 35g plant breasts is added Bacillus seals after mixing, and keeping the temperature 24 hours at 35 DEG C ferments, and the product after fermentation is 24 hours dry at 50 DEG C, 120 DEG C of sterilization 10s, crush, and cross 15 mesh sieve, obtain quinoa white fungus powder.
Embodiment 7
It is substantially the same manner as Example 5, it differs only in, in the present embodiment 7, the preparation method of quinoa white fungus powder is as follows： It is 15% that white fungus, which is dried at 50 DEG C to water content, is crushed, and crosses 10 mesh sieve, obtains white fungus powder, and 300g quinoas, 300g water are mixed Afterwards, 85 DEG C are warming up to, 40 minutes are kept the temperature at 85 DEG C, is cooled to 35 DEG C, 200g white fungus powder, 1.5g citric acids, 35g plant breasts is added Bacillus seals after mixing, and keeping the temperature 72 hours at 35 DEG C ferments, and the product after fermentation is 24 hours dry at 50 DEG C, 120 DEG C of sterilization 10s, crush, and cross 15 mesh sieve, obtain quinoa white fungus powder.
Embodiment 8
A kind of Chinese medicine composition with antitumaous effect, includes the raw material of following weight parts：60 parts of Radix Salviae Miltiorrhizae, 25 parts of Chinese yam, 20 parts of Rhizoma Atractylodis Macrocephalae, 45 parts of the bletilla striata, 35 parts of black nightshade, horse hand over 30 parts of youngster, 35 parts of rhizome of chuanxiong, 8 parts of rheum officinale, 20 parts of the root of bidentate achyranthes, 5 parts of Paris polyphylla, reed root 8 Part, 10 parts of cordate houttuynia, 25 parts of complex polysaccharide, 55 parts of quinoa white fungus powder, 25 parts of passionflower fruit powder, 25 parts of white bur powder.
The preparation method of complex polysaccharide is the same as embodiment 2.
The preparation method of quinoa white fungus powder is the same as embodiment 5.
The preparation method of passionflower fruit powder is as follows：Passionfruit is removed the peel, by the 300g passionfruits after peeling with water by material Liquor ratio is 1:5min is homogenized with the rotating speed of 15000 turns/min after 1g/mL mixing, passionflower pulp is obtained, into passionflower pulp 0.15g cellulases, 0.2g pectases is added, is warming up to 38 DEG C, keeps the temperature 3 hours at 38 DEG C, be warming up to 95 DEG C, is kept the temperature at 95 DEG C 10 minutes, 40 DEG C are cooled to, 100g maltodextrins are added and are uniformly mixed, spray drying obtains the passionflower that water content is 5wt% Fruit powder, the inlet air temperature of spray drying：160 DEG C, leaving air temp is 85 DEG C.The preparation method of white bur powder is as follows：300g is white Bur is 1 by solid-liquid ratio with water:6min is homogenized with the rotating speed of 15000 turns/min after 1g/mL mixing, is added in being starched to white bur 10g citric acids, 0.5g sodium chloride, 0.2g pectases, are warming up to 38 DEG C, keep the temperature 4 hours at 38 DEG C, 85 DEG C are warming up to, at 85 DEG C Heat preservation 7 minutes is cooled to 40 DEG C, and 75g maltodextrins are added and are uniformly mixed, and spray drying obtains the white thorn that water content is 5wt% Fruit powder, the inlet air temperature of spray drying：155 DEG C, leaving air temp is 85 DEG C.
The preparation method of Chinese medicine composition with antitumaous effect：Each raw material is crushed respectively, crosses 30 mesh sieve, after crushing Raw material be uniformly mixed to obtain the final product.
Comparative example 1
A kind of Chinese medicine composition with antitumaous effect, includes the raw material of following weight parts：60 parts of Radix Salviae Miltiorrhizae, 25 parts of Chinese yam, 20 parts of Rhizoma Atractylodis Macrocephalae, 45 parts of the bletilla striata, 35 parts of black nightshade, horse hand over 30 parts of youngster, 35 parts of rhizome of chuanxiong, 8 parts of rheum officinale, 20 parts of the root of bidentate achyranthes, 5 parts of Paris polyphylla, reed root 8 Part, 10 parts of cordate houttuynia, 25 parts of complex polysaccharide, 55 parts of quinoa white fungus powder, 50 parts of passionflower fruit powder.
The preparation method of complex polysaccharide is the same as embodiment 2.
The preparation method of quinoa white fungus powder is the same as embodiment 5.
The preparation method of passionflower fruit powder is the same as embodiment 8.
The preparation method of Chinese medicine composition with antitumaous effect：Each raw material is crushed respectively, crosses 30 mesh sieve, after crushing Raw material be uniformly mixed to obtain the final product.
Comparative example 2
A kind of Chinese medicine composition with antitumaous effect, includes the raw material of following weight parts：60 parts of Radix Salviae Miltiorrhizae, 25 parts of Chinese yam, 20 parts of Rhizoma Atractylodis Macrocephalae, 45 parts of the bletilla striata, 35 parts of black nightshade, horse hand over 30 parts of youngster, 35 parts of rhizome of chuanxiong, 8 parts of rheum officinale, 20 parts of the root of bidentate achyranthes, 5 parts of Paris polyphylla, reed root 8 Part, 10 parts of cordate houttuynia, 25 parts of complex polysaccharide, 55 parts of quinoa white fungus powder, 50 parts of white bur powder.
The preparation method of complex polysaccharide is the same as embodiment 2.
The preparation method of quinoa white fungus powder is the same as embodiment 5.
The preparation method of white bur powder is the same as embodiment 8.
The preparation method of Chinese medicine composition with antitumaous effect：Each raw material is crushed respectively, crosses 30 mesh sieve, after crushing Raw material be uniformly mixed to obtain the final product.
Test case
The Chinese medicine composition with antitumaous effect of embodiment 1-8, comparative example 1-2 are subjected to cancer cell vigor test, surveyed Test result is shown in Table 1.
Table 1：Cancer cell vigor test result table
(%) for 24 hours 48h (%) 72h (%)
Embodiment 1 96 66 55
Embodiment 2 83 52 45
Embodiment 3 88 54 49
Embodiment 4 93 59 52
Embodiment 5 72 45 35
Embodiment 6 76 48 39
Embodiment 7 80 50 42
Embodiment 8 52 37 26
Comparative example 1 69 42 33
Comparative example 2 65 40 32
The Chinese medicine composition with antitumaous effect of embodiment 1-8, comparative example 1-2 are subjected to Thymus and Spleen index Test, test result are shown in Table 2.
Table 2：Thymus and Spleen index test result table
The Chinese medicine composition with antitumaous effect of embodiment 8, comparative example 1-2 is carried out macrophage ability of eating to survey Examination, test result are shown in Table 3.
Table 3：Macrophage is eaten aptitude tests result table
Phagocytic index α
Embodiment 8 5.87±0.18
Comparative example 1 5.34±0.11
Comparative example 2 5.39±0.12
Normal group 6.23±0.14
Model group 4.76±0.19
The Chinese medicine composition with antitumaous effect of the present invention selects Chinese herbal medicines rationally to match according to Chinese medicine combination principle 5, auxiliary addition plant polyose, quinoa white fungus, fruit powder functional component can reduce cancer cell vigor, inhibit growth of cancer cells and expansion It dissipates, enhances Immune Organs Index, improve macrophage and eat ability, to strengthen immunity, reach anti-cancer and anticancer effect.
The preferred embodiment of the present invention has been described in detail above.It should be appreciated that those skilled in the art without It needs creative work according to the present invention can conceive and makes many modifications and variations.Therefore, all technologies in the art Personnel are available by logical analysis, reasoning, or a limited experiment on the basis of existing technology under this invention's idea Technical solution, all should be in the protection domain being defined in the patent claims.

Claims (8)

1. a kind of Chinese medicine composition with antitumaous effect, which is characterized in that including following raw materials：It is Radix Salviae Miltiorrhizae, Chinese yam, Rhizoma Atractylodis Macrocephalae, white Splendid achnatherum, black nightshade, Ma Jiaoer, rhizome of chuanxiong, rheum officinale, the root of bidentate achyranthes, Paris polyphylla, reed root, cordate houttuynia.
2. a kind of Chinese medicine composition with antitumaous effect, which is characterized in that the raw material including following weight parts：Radix Salviae Miltiorrhizae 40-80 Part, 15-30 parts of Chinese yam, 10-30 parts of Rhizoma Atractylodis Macrocephalae, 30-60 parts of the bletilla striata, 30-60 parts of black nightshade, horse hand over 10-40 parts of youngster, 15-45 parts of rhizome of chuanxiong, 5-10 parts of rheum officinale, 10-30 parts of the root of bidentate achyranthes, 3-10 parts of Paris polyphylla, 3-10 parts of reed root, 5-15 parts of cordate houttuynia.
3. a kind of Chinese medicine composition with antitumaous effect, which is characterized in that the raw material including following weight parts：Radix Salviae Miltiorrhizae 40-80 Part, 15-30 parts of Chinese yam, 10-30 parts of Rhizoma Atractylodis Macrocephalae, 30-60 parts of the bletilla striata, 30-60 parts of black nightshade, horse hand over 10-40 parts of youngster, 15-45 parts of rhizome of chuanxiong, 5-10 parts of rheum officinale, 10-30 parts of the root of bidentate achyranthes, 3-10 parts of Paris polyphylla, 3-10 parts of reed root, 5-15 parts of cordate houttuynia, 15-45 parts of complex polysaccharide.
4. a kind of Chinese medicine composition with antitumaous effect, which is characterized in that the raw material including following weight parts：Radix Salviae Miltiorrhizae 40-80 Part, 15-30 parts of Chinese yam, 10-30 parts of Rhizoma Atractylodis Macrocephalae, 30-60 parts of the bletilla striata, 30-60 parts of black nightshade, horse hand over 10-40 parts of youngster, 15-45 parts of rhizome of chuanxiong, 5-10 parts of rheum officinale, 10-30 parts of the root of bidentate achyranthes, 3-10 parts of Paris polyphylla, 3-10 parts of reed root, 5-15 parts of cordate houttuynia, 15-45 parts of complex polysaccharide, lamb's-quarters 40-70 parts of wheat white fungus powder.
5. a kind of Chinese medicine composition with antitumaous effect, which is characterized in that the raw material including following weight parts：Radix Salviae Miltiorrhizae 40-80 Part, 15-30 parts of Chinese yam, 10-30 parts of Rhizoma Atractylodis Macrocephalae, 30-60 parts of the bletilla striata, 30-60 parts of black nightshade, horse hand over 10-40 parts of youngster, 15-45 parts of rhizome of chuanxiong, 5-10 parts of rheum officinale, 10-30 parts of the root of bidentate achyranthes, 3-10 parts of Paris polyphylla, 3-10 parts of reed root, 5-15 parts of cordate houttuynia, 15-45 parts of complex polysaccharide, lamb's-quarters 40-70 parts of wheat white fungus powder, 10-40 parts of passionflower fruit powder, 10-40 parts of white bur powder.
6. the Chinese medicine composition with antitumaous effect according to any one of claim 3-5, which is characterized in that described multiple The preparation method for closing polysaccharide is as follows：Agricus blazei, radix polygonati officinalis, moringa seeds are smashed it through into 10-20 mesh sieve respectively, by the Ji Song after crushing Young pilose antler, radix polygonati officinalis, moringa seeds are (1-3) in mass ratio：(1-3)：(1-3) is mixed to get mixture, adds into 400-800g mixtures Enter 1000-2000g ether, being 30-100 revs/min in 30-35 DEG C, rotating speed stirs 3-15 minutes, crosses 50-100 mesh sieve, obtains First-time filtrate and a filter cake, a filter cake is 20-45 minutes dry at 40-60 DEG C, 3- is added in a filter cake after drying In 8L water, frequency is 20-40KHz, power is 300-500W condition ultrasonic extractions 40-90 minutes, 200-500 mesh sieves excessively, obtains To secondary filtrate and secondary filter cake, the 30-65% that secondary filtrate is concentrated by evaporation at 50-75 DEG C to original volume obtains concentrate, Ethyl alcohol is added into concentrate, the volume fraction of ethyl alcohol is made to reach 65-90%, stands 12-36 hours after mixing, with 3000-8000 revs/min of rotating speed centrifuges 10-30 minutes, and 0.04-0.08MPa, 40-60 DEG C of dryings are deposited in by what centrifugation obtained 6-24 hours, obtain complex polysaccharide.
7. the preparation method of the Chinese medicine composition with antitumaous effect according to claim 1-6：Each raw material is distinguished into powder Broken, 30-50 mesh excessively sieves, and the raw material after crushing is uniformly mixed to obtain the final product.
8. a kind of Chinese medicine composition with antitumaous effect according to claim 1-6 is preparing antitumor, anticancer medicine Purposes in product.
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Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
何清湖等: "《肿瘤良方大全》", 31 January 2016, 山西科学技术出版社 *
宗邵峰: "《一针见穴：手指上的医院》", 31 December 2017, 云南科技出版社 *
王居祥等: "《中医肿瘤治疗学》", 31 March 2014, 中国中医药出版社 *

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