CN108504714A - A method of comprehensive process processing being carried out to chicken courage using biological enzyme - Google Patents
A method of comprehensive process processing being carried out to chicken courage using biological enzyme Download PDFInfo
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- CN108504714A CN108504714A CN201810144042.0A CN201810144042A CN108504714A CN 108504714 A CN108504714 A CN 108504714A CN 201810144042 A CN201810144042 A CN 201810144042A CN 108504714 A CN108504714 A CN 108504714A
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Abstract
The invention discloses a kind of methods carrying out comprehensive process processing to chicken courage using biological enzyme, it is filtered after the chicken courage heating of freezing is thawed, filtered bile is into hydrolysis kettle, cholate is added and hydrolyzes bacterial enzyme, using biological enzyme have it is efficient, single-minded, reaction environment is safe and environment-friendly the advantages that, complexing bile acid is hydrolyzed to the bile in chicken courage.The leftover bits and pieces after hydrolysis is handled simultaneously, utilizes biological enzyme hydrolysis, concentrated spray to obtain animal feed protein additive albumen, courage skin and its stearic acid of adherency in bile.Living resources are not only farthest utilized, and operating process three wastes generate, also there are preferable social and economic effects while environmentally protective.
Description
Technical field
The present invention relates to a kind of methods carrying out comprehensive process processing to chicken courage using biological enzyme, more particularly to a kind of profit
With biological enzyme hydrolysis be complexed chicken courage in bile acid and using separation bile after Ji Danpideng animal tissues prepare animal raise
Expect the method for protein additive.
Background technology
Chicken courage is a kind of convenient source of industrial production bile acid, and mode existing for bile acid is ox sulphur in the bile of chicken courage
The complexing sodium salt that acid and glycine are combined with bile acid is extracted and is detached for convenience in process of production, need to be by taurine
It is detached from glycine and bile acid.And conventional extracting method is that a large amount of sodium hydroxide is added in bile, protects boiling within 24 hours
Flow back saponification, and taurine and glycine are detached with bile acid, add hydrochloric acid, bile acid is precipitated, bile is collected after filtering
Acid, then carries out the extraction of single bile acid and detaches.Above-mentioned common process is hydrolyzed using highly basic, and acid out goes out, and is consumed a large amount of
Harmful influence sodium hydroxide and hydrochloric acid, the spent acid and buck generated after hydrolysis form huge environmental protection pressure and risk.And water
In solution preocess, because a large amount of Proteins contained in bile take part in hydrolysis, when high temperature reflux, discharges ammonia, can also greatly increase
The cost of environmental protection treatment.In addition, a large amount of courage skin and the subsidiary animal tissue of courage skin can be formed after chicken courage separation bile, this
It can become a huge environmentally friendly hidden danger again containing oil, albumen and cystic element, not in time processing in a little leftover bits and pieces.Therefore,
So that these limited living resources is fully used, is that people urgently want to solve the problems, such as.
Invention content
In view of the above-mentioned problems, carrying out comprehensive process processing to chicken courage using biological enzyme the object of the present invention is to provide a kind of
Method, using biological enzyme have it is efficient, single-minded, reaction environment is safe and environment-friendly the advantages that, the bile in chicken courage is hydrolyzed
Bile acid is complexed, while preparing animal feed protein additive after handling the leftover bits and pieces after hydrolysis, environmental protection but also can not only take
Obtain preferable economic well-being of workers and staff.
To achieve the goals above, the present invention adopts the following technical scheme that：
A method of comprehensive process processing being carried out to chicken courage using biological enzyme, which is characterized in that the step of working process is as follows：
（1）The chicken bladder cut is put into stainless steel defrosting kettle, passed through by the chicken courage for taking freezing through animal tissue sections machine-cut piece
Steam heating, stirring are warming up to 40-45 DEG C；Then the material to have thawed is put into suction filtration tank filtering, filtered bile enters
Bile acid hydrolysis kettle；Suction filtration finishes, clear up remaining courage skin wash with water it is rear spare.
（2）The chilled water that 7 DEG C are inputted into bile acid hydrolysis kettle interlayer, stirs the bile in bile acid hydrolysis kettle, makes courage
The temperature of juice is down to 35-40 DEG C；Then a concentration of 10% aqueous hydrochloric acid solution is slowly added dropwise, until the pH value in hydrolysis kettle is
When 5.1-5.7, cholate is added in hydrolysis kettle and hydrolyzes bacterial enzyme, the addition that cholate hydrolyzes bacterial enzyme is Amount of Bile in hydrolysis kettle
0.1-0.2%, continue to be slowly added dropwise 10% aqueous hydrochloric acid solution, for control pH value between 5.1-5.7, the reaction time is that 2-5 is small
When, sampling is detected by HPLC, percent hydrolysis 99.5-100%.
（3）By step（2）The middle bile by hydrolysis process is warming up to 50-60 DEG C, and a concentration of 20% is added dropwise while stirring
Aqueous hydrochloric acid solution, adjusting pH value are 2.5-3.5, and bile anaerobic hydrolysate is inputted polypropylene plate and frame filter press after 30 minutes, are received
Collect mother liquor into mother liquor hydrolysis kettle, collects the free bile acid in sheet frame to get bile acid crude.
（4）The stirring of mother liquor hydrolysis kettle is opened, 7 DEG C of chilled water is inputted to mother liquor hydrolysis kettle interlayer, is made in mother liquor hydrolysis kettle
Mother liquor cool to 37-42 DEG C, put into step（1）In cleaned spare courage skin, with a concentration of 10% sodium hydroxide water
When solution adjusting pH value is 6.8-7.2, input albumen enzyme powder and fatty enzyme powder, the wherein input amount of albumen enzyme powder are step（1）
The input amount of the 0.02-0.05% of taken frozen chicken courage, fatty enzyme powder are step（1）The 0.05- of taken frozen chicken courage
0.1%, 37-42 DEG C of temperature is kept, controls pH value between 6.8-7.2 with a concentration of 10% sodium hydrate aqueous solution, stirring
Hydrolysis 4 hours.
（5）By step（4）In mother liquor hydrolyzate through 100 mesh tiffanys filtering after, into economic benefits and social benefits be concentrated in vacuo kettle, in vacuum
Degree -0.9MP under conditions of 70-80 DEG C of temperature, is concentrated into the fluid cream that water content is 30%.Wherein, what tiffany filtered out is a small amount of
Impurity is 55-70 into incinerator processing, condensed water COD, and no coloration can arrange outside.
（6）By step（5）Fluid cream after middle concentration adds after dry up to animal feed albumen into spray dryer
Agent.After testing, the total free amino acid content in animal feed protein additive is greater than or equal to 70%, and total fatty acid content is big
In or be equal to 20%, moisture be less than or equal to 5.0%, other materials content be less than or equal to 5%, wherein other materials be courage
Sterol, phosphatide, inorganic salts.
The beneficial effects of the present invention are：
1, it is hydrolyzed by the biological hydrolysis enzymes extraction highly basic of carrier of Escherichia coli using cholate hydrolysis bacterial enzyme is this, room temperature is normal
Pressure, it is a small amount of using dangerous chemicals, operating safety risks are reduced, energy consumption is greatly decreased, chemical industry cost of supplementary product is greatly reduced, substantially
Improve production efficiency.
2, biological enzyme hydrolysis, concentrated spray is utilized to obtain animal feeding albumen, courage skin and its stearic acid of adherency in bile
Expect protein additive.Living resources are not only farthest utilized, and operating process three wastes generate, while environmentally protective
Also there are preferable social and economic effects.
3, before hydrolysis process, just remaining courage skin is detached by filtering so that a large amount of albumen contained in bile
Composition is not involved in hydrolysis, and when high temperature reflux will not then discharge a large amount of ammonias, environmental protection but also can substantially reduce environmental protection treatment
Cost.
Specific implementation mode
To make the objectives, technical solutions, and advantages of the present invention clearer, below in conjunction with specific embodiment, to this hair
Bright method is further described.
Embodiment one：
A method of comprehensive process processing being carried out to chicken courage using biological enzyme, the step of working process is as follows：
（1）1.3 tons of chicken in jelly courages are taken, through animal tissue sections machine-cut piece, the chicken in jelly bladder cut is put into 2000L stainless steels and is thawed
Kettle, steam heating, stirring are warming up to 40 DEG C.Then the material to have thawed is put to 2M2Suction filtration tank filtering, filtered bile
Into bile acid hydrolysis kettle, suction filtration finishes, and courage skin is cleaned with 100L water, spare.
（2）7 DEG C of chilled waters are inputted into bile acid hydrolysis kettle interlayer, are stirred the bile in bile acid hydrolysis kettle, are made bile
Be cooled to 35.5 DEG C, a concentration of 10% aqueous hydrochloric acid solution is slowly added dropwise, until hydrolysis kettle in pH value be 5.5 when, input
1.4 kilograms of cholate hydrolyze bacterial enzyme, continue that 10% aqueous hydrochloric acid solution is slowly added dropwise, control pH value is between 5.1-5.7, reaction 4
Hour, sampling is detected by HPLC, and conjugated bile acids, percent hydrolysis 100% is not detected.
（3）Concentration is added dropwise for 20% aqueous hydrochloric acid solution in hydrolysis kettle, adjusts pH value 2.6, is warming up to 55 DEG C, stirring 30
After minute, bile anaerobic hydrolysate is inputted into 10M using pump2Polypropylene plate and frame filter press is collected mother liquor into mother liquor hydrolysis kettle, is received
Collect the free bile acid in sheet frame, pack is weighed to obtain 181 kilograms, spare bile acid separation production.
（4）The stirring of mother liquor hydrolysis kettle is opened, 7 DEG C of chilled water is inputted to mother liquor hydrolysis kettle interlayer, is made in mother liquor hydrolysis kettle
Mother liquor cool to 38.5 DEG C, step is put into after stirring（1）In the courage skin that has cleaned, with a concentration of 10% sodium hydroxide water
Solution adjusts pH value 7.0, puts into 0.52 kilogram of albumen enzyme powder, 0.9 kilogram of fatty enzyme powder, control temperature is between 37-42 DEG C, control
PH value 6.8-7.2 processed controls pH value between 6.8-7.2, stirring hydrolysis 4 hours with a concentration of 10% sodium hydrate aqueous solution.
（5）Mother liquor hydrolyzate is filtered through 100 mesh tiffanys, and kettle is concentrated in vacuo into economic benefits and social benefits, is -0.9MP, temperature in vacuum degree
Under conditions of 70 degree, it is concentrated into the fluid cream that water content is 30%.GIFfany filters out a small amount of impurity into incinerator processing, concentration
Water COD is 55, and no coloration can arrange outside.
（6）By the fluid cream after concentration into spray dryer, 201 kilograms of animal feed protein additive is obtained after dry.Through
It detects, total free amino acid content 71% in animal feed protein additive, total fatty acids 23%, moisture 3.7% is other
Content of material（Cholesterol, phosphatide, inorganic salts etc.）2.3%.
Embodiment two：
A method of comprehensive process processing being carried out to chicken courage using biological enzyme, the step of working process is as follows：
（1）1.15 tons of chicken in jelly courages are taken, through animal tissue sections machine-cut piece, the chicken in jelly bladder cut is put into 2000L stainless steel solutions
Freeze kettle, steam heating, stirring is warming up to 43 DEG C.Then the material to have thawed is put to 2M2Suction filtration tank filtering, filtered courage
Juice enters bile acid hydrolysis kettle, and suction filtration finishes, and courage skin is cleaned with 100L water, spare.
（2）7 DEG C of chilled waters are inputted into bile acid hydrolysis kettle interlayer, are stirred the bile in bile acid hydrolysis kettle, are made bile
Be cooled to 38.5 DEG C, a concentration of 10% aqueous hydrochloric acid solution is slowly added dropwise, until hydrolysis kettle in pH value be 5.3 when, input
1.2 kilograms of cholate hydrolyze bacterial enzyme, continue that 10% aqueous hydrochloric acid solution is slowly added dropwise, control pH value is between 5.1-5.7, reaction
3.5 hours, sampling was detected by HPLC, percent hydrolysis 99.92%.
（3）Concentration is added dropwise for 20% aqueous hydrochloric acid solution in hydrolysis kettle, adjusts pH value 2.9, is warming up to 59 DEG C, stirring 30
After minute, bile anaerobic hydrolysate is inputted into 10M using pump2Polypropylene plate and frame filter press is collected mother liquor into mother liquor hydrolysis kettle, is received
Collect the free bile acid in sheet frame, pack is weighed to obtain 163.3 kilograms, spare bile acid separation production.
（4）The stirring of mother liquor hydrolysis kettle is opened, 7 DEG C of chilled water is inputted to mother liquor hydrolysis kettle interlayer, is made in mother liquor hydrolysis kettle
Mother liquor cool to 38.5 DEG C, step is put into after stirring（1）In the courage skin that has cleaned, with a concentration of 10% sodium hydroxide water
Solution adjusts pH value 7.0, puts into 0.38 kilogram of albumen enzyme powder, 0.8 kilogram of fatty enzyme powder, control temperature is between 37-42 DEG C, control
PH value 6.8-7.2 processed controls pH value between 6.8-7.2, stirring hydrolysis 4 hours with a concentration of 10% sodium hydrate aqueous solution.
（5）Mother liquor hydrolyzate is filtered through 100 mesh tiffanys, and kettle is concentrated in vacuo into economic benefits and social benefits, is -0.9MP, temperature in vacuum degree
Under conditions of 75 degree, it is concentrated into the fluid cream that water content is 30%.GIFfany filters out a small amount of impurity into incinerator processing, concentration
Water COD is 65, and no coloration can arrange outside.
（6）By the fluid cream after concentration into spray dryer, 235.7 kilograms of animal feed protein additive is obtained after dry.
After testing, total free amino acid content 70.8% in animal feed protein additive, total fatty acids 22.5%, moisture are
4.1%, other materials content（Cholesterol, phosphatide, inorganic salts etc.）2.6%.
Embodiment three：
A method of comprehensive process processing being carried out to chicken courage using biological enzyme, the step of working process is as follows：
（1）1.21 tons of chicken in jelly courages are taken, through animal tissue sections machine-cut piece, the chicken in jelly bladder cut is put into 2000L stainless steel solutions
Freeze kettle, steam heating, stirring is warming up to 45 DEG C.Then the material to have thawed is put to 2M2Suction filtration tank filtering, filtered courage
Juice enters bile acid hydrolysis kettle, and suction filtration finishes, and courage skin is cleaned with 100L water, spare.
（2）7 DEG C of chilled waters are inputted into bile acid hydrolysis kettle interlayer, are stirred the bile in bile acid hydrolysis kettle, are made bile
Be cooled to 36.3 DEG C, a concentration of 10% aqueous hydrochloric acid solution is slowly added dropwise, until hydrolysis kettle in pH value be 5.6 when, input
1.3 kilograms of cholate hydrolyze bacterial enzyme, continue that 10% aqueous hydrochloric acid solution is slowly added dropwise, control pH value is between 5.1-5.7, reaction
3.5 hours, sampling was detected by HPLC, and conjugated bile acids, percent hydrolysis 100% is not detected.
（3）Concentration is added dropwise for 20% aqueous hydrochloric acid solution in hydrolysis kettle, adjusts pH value 3.5, is warming up to 58 DEG C, stirring 30
After minute, bile anaerobic hydrolysate is inputted into 10M using pump2Polypropylene plate and frame filter press is collected mother liquor into mother liquor hydrolysis kettle, is received
Collect the free bile acid in sheet frame, pack is weighed to obtain 179 kilograms, spare bile acid separation production.
（4）The stirring of mother liquor hydrolysis kettle is opened, 7 DEG C of chilled water is inputted to mother liquor hydrolysis kettle interlayer, is made in mother liquor hydrolysis kettle
Mother liquor cool to 38.5 DEG C, step is put into after stirring（1）In the courage skin that has cleaned, with a concentration of 10% sodium hydroxide water
Solution adjusts pH value 7.0, puts into 0.47 kilogram of albumen enzyme powder, and 0.85 kilogram of fatty enzyme powder controls temperature between 37-42 DEG C,
PH value 6.8-7.2 is controlled, controls pH value between 6.8-7.2 with a concentration of 10% sodium hydrate aqueous solution, stirring hydrolysis 4 is small
When.
（5）Mother liquor hydrolyzate is filtered through 100 mesh tiffanys, and kettle is concentrated in vacuo into economic benefits and social benefits, is -0.9MP, temperature in vacuum degree
Under conditions of 80 degree, it is concentrated into the fluid cream that water content is 30%.GIFfany filters out a small amount of impurity into incinerator processing, concentration
Water COD is 70, and no coloration can arrange outside.
（6）By the fluid cream after concentration into spray dryer, 217.8 kilograms of animal feed protein additive is obtained after dry.
After testing, total free amino acid content 73% in animal feed protein additive, total fatty acids 21.5%, moisture 4.0%,
Other materials content（Cholesterol, phosphatide, inorganic salts etc.）1.5%.
Claims (9)
1. a kind of method carrying out comprehensive process processing to chicken courage using biological enzyme, which is characterized in that the step of working process such as
Under：
（1）The chicken bladder cut is put into stainless steel defrosting kettle, passed through by the chicken courage for taking freezing through animal tissue sections machine-cut piece
Steam heats, and the material to have thawed is put into suction filtration tank filtering after stirring, and filtered bile enters bile acid hydrolysis kettle；It filters
Finish, clear up remaining courage skin wash with water it is rear spare；
（2）The chilled water that 7 DEG C are inputted into bile acid hydrolysis kettle interlayer, stirs the bile in bile acid hydrolysis kettle, makes bile
Temperature reduces；Then a concentration of 10% aqueous hydrochloric acid solution is slowly added dropwise, until when the pH value in hydrolysis kettle is 5.1-5.7, in water
It solves and cholate hydrolysis bacterial enzyme is added in kettle, continue that 10% aqueous hydrochloric acid solution is slowly added dropwise, control pH value is between 5.1-5.7, instead
It is 2-5 hours between seasonable, sampling is detected by HPLC, percent hydrolysis 99.5-100%；
（3）By step（2）After the middle bile heating by hydrolysis process, a concentration of 20% aqueous hydrochloric acid solution is added dropwise while stirring,
Adjusting pH value is 2.5-3.5, and bile anaerobic hydrolysate is inputted polypropylene plate and frame filter press after 30 minutes, collects mother liquor into mother liquor
Hydrolysis kettle collects the free bile acid in sheet frame to get bile acid crude；
（4）The stirring of mother liquor hydrolysis kettle is opened, 7 DEG C of chilled water is inputted to mother liquor hydrolysis kettle interlayer, makes the mother in mother liquor hydrolysis kettle
Liquid cooling is but cooled to 37-42 DEG C, puts into step（1）In cleaned spare courage skin, with a concentration of 10% sodium hydrate aqueous solution
When adjusting pH value is 6.8-7.2, input albumen enzyme powder and fatty enzyme powder, the temperature of 37-42 DEG C of holding, with a concentration of 10% hydrogen
Aqueous solution of sodium oxide controls pH value between 6.8-7.2, stirring hydrolysis 4 hours；
（5）By step（4）In mother liquor hydrolyzate through 100 mesh tiffanys filtering after, into economic benefits and social benefits be concentrated in vacuo kettle, be concentrated into aqueous
The fluid cream that amount is 30%；
（6）By step（5）Fluid cream after middle concentration is into spray dryer, up to animal feed protein additive after drying；Through
It detects, the total free amino acid content in animal feed protein additive is greater than or equal to 70%, and total fatty acid content is more than or waits
In 20%, moisture is less than or equal to 5.0%, and other materials content is less than or equal to 5%.
2. the method as described in claim 1 for carrying out comprehensive process processing to chicken courage using biological enzyme, which is characterized in that step
（1）Temperature after middle chicken bladder is heated by steam is 40-45 DEG C.
3. the method as described in claim 1 for carrying out comprehensive process processing to chicken courage using biological enzyme, which is characterized in that step
（2）After middle addition chilled water, the temperature of bile is reduced to 35-40 DEG C in bile acid hydrolysis kettle.
4. the method as described in claim 1 for carrying out comprehensive process processing to chicken courage using biological enzyme, which is characterized in that step
（2）The addition of middle cholate hydrolysis bacterial enzyme is the 0.1-0.2% of Amount of Bile in hydrolysis kettle.
5. the method as described in claim 1 for carrying out comprehensive process processing to chicken courage using biological enzyme, which is characterized in that step
（3）The temperature of bile is 50-60 DEG C after middle heating.
6. the method as described in claim 1 for carrying out comprehensive process processing to chicken courage using biological enzyme, which is characterized in that step
（4）The input amount of middle albumen enzyme powder is step（1）The input amount of the 0.02-0.05% of taken frozen chicken courage, fatty enzyme powder are step
Suddenly（1）The 0.05-0.1% of taken frozen chicken courage.
7. the method as described in claim 1 for carrying out comprehensive process processing to chicken courage using biological enzyme, which is characterized in that step
（5）It is -0.9MP that middle economic benefits and social benefits, which are concentrated in vacuo the vacuum degree in kettle, and temperature when being concentrated in kettle is 70-80 DEG C.
8. the method as claimed in claim 1 or 7 for carrying out comprehensive process processing to chicken courage using biological enzyme, which is characterized in that
Step（5）The impurity that middle tiffany filters out is 55-70 into incinerator processing, condensed water COD, and no coloration can arrange outside.
9. the method as described in claim 1 for carrying out comprehensive process processing to chicken courage using biological enzyme, which is characterized in that step
（6）In other materials be cholesterol, phosphatide and inorganic salts.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110771677A (en) * | 2019-12-08 | 2020-02-11 | 常德云港生物科技有限公司 | Chicken gallbladder thawing and filtering device for pretreatment of chicken gallbladder processing by biological enzyme |
| CN111334553A (en) * | 2020-03-26 | 2020-06-26 | 山东中京生物科技有限公司 | Process for producing bile paste by enzyme method |
| CN112322684A (en) * | 2020-11-09 | 2021-02-05 | 湖南夏盛酶技术有限公司 | Method for extracting bile acid by using high-efficiency enzyme of oxgall |
| CN113999886A (en) * | 2021-12-06 | 2022-02-01 | 江苏省农业科学院 | Method for extracting chicken bile acid by one-pot enzymolysis |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1442076A (en) * | 2003-04-01 | 2003-09-17 | 朱德高 | Method of processing animal feed using enzymatic hydrolysis |
| CN102690856A (en) * | 2012-05-30 | 2012-09-26 | 绵阳劲柏生物科技有限责任公司 | Process using microbial solution to prepare free bile acid |
-
2018
- 2018-02-12 CN CN201810144042.0A patent/CN108504714A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1442076A (en) * | 2003-04-01 | 2003-09-17 | 朱德高 | Method of processing animal feed using enzymatic hydrolysis |
| CN102690856A (en) * | 2012-05-30 | 2012-09-26 | 绵阳劲柏生物科技有限责任公司 | Process using microbial solution to prepare free bile acid |
Non-Patent Citations (3)
| Title |
|---|
| G. A. D. HASLEWOOD: "Bile salts of germ-free domestic fowl and pigs", 《BIOCHEM. J.》 * |
| 刘淑兰等: "以鸡胆为原料制取熊去氧胆酸", 《应用科技》 * |
| 岳婷婷等: "胆盐水解酶的研究现状", 《中国乳品工业》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110771677A (en) * | 2019-12-08 | 2020-02-11 | 常德云港生物科技有限公司 | Chicken gallbladder thawing and filtering device for pretreatment of chicken gallbladder processing by biological enzyme |
| CN111334553A (en) * | 2020-03-26 | 2020-06-26 | 山东中京生物科技有限公司 | Process for producing bile paste by enzyme method |
| CN112322684A (en) * | 2020-11-09 | 2021-02-05 | 湖南夏盛酶技术有限公司 | Method for extracting bile acid by using high-efficiency enzyme of oxgall |
| CN113999886A (en) * | 2021-12-06 | 2022-02-01 | 江苏省农业科学院 | Method for extracting chicken bile acid by one-pot enzymolysis |
| CN113999886B (en) * | 2021-12-06 | 2023-07-04 | 江苏省农业科学院 | Method for extracting chicken bile acid by one-pot enzymolysis |
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