CN1085039C - Breeding method of Chinese cabbage Aikang No.4 - Google Patents
Breeding method of Chinese cabbage Aikang No.4 Download PDFInfo
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- CN1085039C CN1085039C CN98111283A CN98111283A CN1085039C CN 1085039 C CN1085039 C CN 1085039C CN 98111283 A CN98111283 A CN 98111283A CN 98111283 A CN98111283 A CN 98111283A CN 1085039 C CN1085039 C CN 1085039C
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Abstract
The present invention relates to a method for breeding a new variety of non-heading Chinese cabbage. A yellow male sterile dual-purpose line with short feet is used as a female parent, and a selfing line 93R-37 (Xuekeqing) is used as a male parent for breeding a new variety of short resistant No. 4 with good comprehensive trait. According to field experiments, artificial inoculation identification and indoor analysis and measurement result, the short resistant No. 4 has the advantages of high yield, disease resistance, high quality, high Vc content, low organic acid content, heat resistance, low heat damage index (9.75) and good marketability, wherein the average yield of production test in summer and in autumn respectively reach 42.2 and 63.4 t/hm<2>, the manual-identification disease indexes of TuMV, downy mildew and nigredo are respectively 1.85(HR), 15.48(R) and 10.10(HR), and both the content of blade soluble solids and the content of leaf stalk soluble solids are higher than those of a contrast.
Description
The present invention relates to the selection of Chinese cabbage crossbreed, specifically the seed selection of Chinese cabbage hybrid new breed " Aikang No.4 ".
Chinese cabbage (Brassica campestris ssp.chinensis Makino) occupies critical role in southern china vegetables year-round supply.Chinese cabbage seminar of Agricultural University Of Nanjing has successively bred short assorted No. 1, No. 2, No. 3 and short anti-No. 1, No. 2, No. 3 serial new varieties, and in more than 20 the provinces large scale application in the whole nation.In recent years, the generation of virus disease, downy mildew, black spot is more and more heavier, causes yield and quality to descend to some extent.For this reason, the inventor carries out three anti-breeding of new variety researchs on the basis of original work.
The object of the present invention is to provide the selection of a kind of Chinese cabbage crossbreed, select not only high yield, of fine quality, be suitable for the plantation in summer simultaneously, Chinese cabbage new varieties that can be high temperature resistant, disease-resistant are in order to solve the year-round supply problem that vegetables produce.
The selection of Chinese cabbage crossbreed provided by the present invention, comprise: with the yellow male sterile two-purpose line Chinese cabbage of short pin is female parent, with another inbred line Chinese cabbage be that male parent is hybridized and cultivated crossbreed, it is characterized in that, selecting 93R-37 inbred line (the snow gram is blue or green) Chinese cabbage for use is that male parent is carried out hybrid seeding, promptly breeds Chinese cabbage Aikang No.4 new varieties.The yellow male sterile two-purpose line of short pin that wherein selected female parent is preferably identified by 2～5 TuMV (turnip mosaic disease), downy mildew, the artificial infection of black spot disease resistance and identify naturally in 2～5 fields, male parent preferably pass through the 93R-37 inbred line that identify naturally in 2～5 TuMV, downy mildew, black spot disease resistance artificial infection evaluation and 2～5 fields.
The used yellow male sterile two-purpose line Chinese cabbage of maternal short pin of the present invention is that Chinese cabbage breeding seminar of Agricultural University Of Nanjing breeds, the comprehensive good breeding material of proterties, document sees reference: Cao Shouchun, Li Shijun, the seed selection of fire Chinese cabbage ' short assorted No. one ' and male sterile two-purpose line, the gardening journal, 1981,8 (3); Used male parent 93R-37 inbred line (the snow gram is blue or green) Chinese cabbage is the good landrace of comprehensive proterties, this breeding group is once as breeding material, document sees reference: Ceng Guoping, Cao Shouchun, Chinese cabbage main quality character inheritance effect analysis, the gardening journal, 1997,24 (1), all belong to public, the available kind material of those of ordinary skill.
The Chinese cabbage hybrid new breed Aikang No.4 that adopts the selection of Chinese cabbage crossbreed provided by the present invention to breed is compared with prior art (short preferably assorted No. one, the short anti-contrast of comprehensive proterties), has the following advantages:
1) high yield
1991～1992 years in the campus experimental field carry out 2 year summer, autumn variety comparative test.The result shows, no matter summer or autumn, the output of Aikang No.4 all is significantly higher than contrast short assorted No., short anti-No. (table 1).1992～1993 years in Nanjing Yuhuatai District, Wuxi City, Liyang City with carried out summer, autumn regional test in the school.The result shows, test the summer in 2 years average yield all be significantly higher than short assorted No. one, test autumn average yield all the utmost point be significantly higher than contrast short anti-No. (table 2).Carried out summer, production test in autumn (table 3) in Nanjing Yuhuatai District, Wuxi City, Liyang City in 1992～1993 years, the result shows, in the summer production test, the Aikang No.4 comparison is according to a short assorted volume increase 13.5%～32.3%, and the comparison in autumn is according to a short anti-volume increase 22.2%～27.8%.
2) disease-resistant
Autumn to virus, downy mildew, black spot carry out field and naturally identify virus disease, downy mildew, black spot, the anthracnose of Aikang No.4 1991～1994 year summer.Under the indoors artificial inoculation condition, carried out the evaluation of anti-3 kinds of diseases in seedling stage in 1992～1993 years.Carried out the unified artificial infection disease resistance in the whole nation in 1994 and identify that TuMV, downy mildew are carried out at the Heilungkiang academy of agricultural sciences horticulture, black spot is carried out in Beijing City Agriculture and Forestry Institute plant protection environmental protection.From field resistance qualification result (table 4) as seen, summer and autumn Aikang No.4 TuMV, downy mildew, black spot are all had stronger resistance, summer also anti-anthracnose.The unified artificial infection qualification result in the whole nation shows that Aikang No.4 reaches high anti-level to the resistance of TuMV, black spot, and the resistance of downy mildew is reached disease-resistant level.
3) heat-resisting
(about July 20) sowing when the most hot in summer in 1991～1994 years is investigated the growth potential of Aikang No.4 and dead plant rate and recovery capability.The result shows that Aikang No.4 growth potential is strong, and dead plant rate only is 5.0%; And contrast short assorted No. one dead plant rate up to 28.0%.Recovery capability (representing that with heat injury index computing formula the is as follows) Aikang No.4 of (34～36 ℃) is 9.75 behind the chance high temperature, and short assorted No. one is 42.50.As seen, the warm tolerance of Aikang No.4 is strong especially.
Heat injury index=(∑ rank * this grade strain number/highest level * total strain number) * 100
Heat evil symptom is divided into 0,1,2,3,4 grade.0 grade---plant strain growth is normal, does not have the heat evil to occur; 1 grade---the slight warp of plant young leaves leaf margin; 2 grades---the slight shrinkage of young leaves, leaf margin warp; 3 grades---the shrinkage of blade moderate, blade warp; 4 grades---the serious shrinkage warp of blade, blade is fine strip shape.
4) quality
4.1) commodity is good
The Aikang No.4 plant type is upright, with a tight waist; The number of blade is many, bottle green, oval; Petiole is flat, green white; The heavy 0.49kg (table 5) of individual plant.This kind growth potential is strong, suits in the area plantation of the green stalk of happiness food Chinese cabbage.
4.2) nutritional quality
The Vc content comparison of the every 1kg fresh vegetable of Aikang No.4 is according to improving 5.2%; Dry matter and organic acid content all are lower than contrast; It is short anti-No. one no matter soluble solid is that blade or petiole all are higher than contrast.Blade heavy with petiole anharmonic ratio value be 0.45, a little less than the contrast (table 6).
Embodiment:
Utilize the heterosis breeding technology, with the yellow male sterile two-purpose line of disease-resistant short pin is female parent, the 93R-37 selfing is male parent preparing hybrid combination, and artificial multiresistance associating in the seedling stage evaluation and screening of virus, downy mildew, 3 kinds of diseases of blackspot is carried out in new combination, and the going forward side by side Ji proterties of passing through is selected.In 4 years, identify naturally, determine that this makes up anti-TuMV and downy mildew through 3 times three anti-artificial infection evaluations and 4 fields, and high anti-black spot.Through 2 years varietal yield tests, regional test and production tests, prove not only high yield, heat-resisting of this combination, and commodity is good, has reached the select target of expection, names to be " Aikang No.4 ".In Jiangsu, the demonstration of Shandong, Beijing promotes, and is subjected to production and consumption person's favorable comment deeply.
The cultivation main points: Aikang No.4 can be done summer vegetable, autumn and winter dish and the dish of surviving the winter cultivation in the south of the lower reaches of the Yangtze River, breeding time 70～100d.Summer culture, the late July～the late August sowing, the late August～field planting mid-September, spacing in the rows 16cm, line-spacing 18cm, gather late September～mid-October, output 30000～52500kg/hm
2, it is also remarkable to make vegetable shoots cultivation advantage.The autumn and winter dish and the dish cultivation of surviving the winter, the early September～sowing mid-October, the early October～the late November field planting, spacing in the rows 18cm, line-spacing 20cm, mid-November to next year, early March was gathered, and output can reach 58500～66000kg/hm
2, rich water quality management is with general green vegetables.
Table 1 variety test output
(kg)
Autumn in summer
Kind 19911) 1992
1) 1991
2) 1992
3)
Aikang No.4 6.11a 2.55
a 11.39
a 12.36
a
Short assorted (CK) 5.50b 1.55
b - -
Short anti-(CK)--9.23b 11.04
b
Annotate: the mean value that 1) repeats for 4 times, residential quarter area 1.4m2。
2) mean value that repeats for 4 times, residential quarter area 1.6m2。
3) mean value that repeats for 4 times, residential quarter area 2.3m2。
4) adopt Deng Kenshi SSR method, carry out test of significance.
Table 2 regional testing output
(kg)
Summer1)
1992 1993
Kind
Yuhua District Wuxi City Liyang city average Nanjing farming Yuhua District is average
Aikang No.4 12.45 11.25 15.30 13.00 is learned by sparetime university
a26.88 54.32 40.60
aShort assorted No. (CK) 9.35 7.75 11.30 9.47
b17.54 46.90 32.22
b
Kind autumn
2)
1992 1993
The average Aikang No.4 36.60 34.75 56.70 42.68 in average Yuhua District Wuxi City Liyang City, Yuhua District Wuxi City Liyang City
A14.03 16.40 12.70 14.47
AShort anti-No. (CK) 35.70 26.55 38.16 33.29
B9.10 8.60 10.70 9.47
BAnnotate: the 1) mean value that repeats for 4 times, sub-district area 4.4m
2
2) mean value that repeats for 4 times, sub-district area 4.8m
2
Table 3 production test output
*
Summer (1992)
Liyang City's Yuhua District
The volume increase of variety yield volume increase output
(kg/hm
2) (％) (kg/hm
2) (％)
Aikang No.4 31,701 32.3 52,713 13.5 short assorted No. (CK) 23,958 0 46,428 0
Autumn (1993)
Yuhua District Liyang City Wuxi City
The volume increase of variety yield volume increase output volume increase output
(kg/hm
2) (％) (kg/hm
2) (％) (kg/hm
2) (％)
Aikang No.4 66904.5 27.8 58765.5 25.4 64504.5 22.2 short anti-(CK) 52348.5 0 46869.0 0 52780.5 0
*The production test area is 333.5m
2
The disease resistance of table 4 Aikang No.4
Field resistance is identified
Autumn in summer
Kind
Brassica 2 et 4 downy mildew black spot anthracnose Brassica 2 et 4 downy mildew black spot
％
1) DI
2) ％ DI ％ DI ％ DI ％ DI ％ DI ％ DI
Aikang No.4 36.40 12.60 8.60 5.10 30.10 8.40 34.60 17.80 14.60 8.40 30.20 16.80 28.90 17.90
Short anti-No. (CK) 40.30 20.10 53.60 21.60 36.70 9.10 95.80 37.60------
Short assorted No. one (CK)--------19.60 10.90 38.90 20.60 40.20 30.60
Artificial infection is identified
3)
Brassica 2 et 4 downy mildew black spot
％ DI ％ DI ％ DI
Aikang No.4 30.00 1.85 100.00 15.48 100.00 10.10 two OX-heart, (CK) 100.00 33.95 100.00 33.64--No. 1, Beijing, (CK)----100.00 56.44 annotate: 1) incidence of disease 2) disease index
3) the unified artificial infection in the whole nation is identified.
Table 5 staple appearance character
(1993, unit: cm)
Blade petiole individual plant is heavy
Pluckings the having a large waist of kind plant type plant height dish
Become on the leaf color length width of the number of sheets shape color length the short anti-No. 1 wealthy ovum that fall of short anti-No. 4 upright 24.9 11.0 8.3 22 oblongs with a tight waist of wide scantling g down dark green 21.5 17.0 flat green white 10.1 4.5 6.5 1.2 491
Upright with a tight waist 28.1 10.2 8.0 16 green 27.4 19.4 flat white 7.8 6.2 6.5 1.1 431
(CK) circle
Annotate: data are 20 strain means in the table.
Table 6 nutrient composition content
(1991，Nanjing)
Heavy vitamin C soluble solid (%) raw fiber soluble protein kind dry matter (%) organic acid (%) of blade
The heavy mgkg of petiole
-1Blade petiole mgkg
-1Mgkg
-1Aikang No.4 0.45 346 5.1 5.9 2.6 2.08 44.8 1.21 short anti-No. one 0.52 329 5.3 4.3 1.9 2.38 40.8 1.13 notes: the mean value of 3 repetitions.
Claims (3)
1, the selection of Chinese cabbage crossbreed, comprise: with the yellow male sterile two-purpose line Chinese cabbage of short pin is female parent, with another inbred line Chinese cabbage be that male parent is hybridized and cultivated crossbreed, it is characterized in that selecting 93R-37 inbred line (the snow gram is blue or green) Chinese cabbage for use is that male parent is carried out hybrid seeding.
2, the selection of Chinese cabbage crossbreed according to claim 1, it is characterized in that selected female parent is the short pin Huang male sterile two-purpose line of identifying by 2～5 TuMV (turnip mosaic disease), downy mildew, the artificial infection of black spot disease resistance and identify naturally in 2～5 fields.
3, the selection of Chinese cabbage crossbreed according to claim 1 and 2, it is characterized in that selected male parent is the 93R-37 inbred line of identifying by 2～3 TuMV, downy mildew, the artificial infection of black spot disease resistance and identify naturally in 2～5 fields.
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| Application Number | Priority Date | Filing Date | Title |
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| CN98111283A CN1085039C (en) | 1998-05-06 | 1998-05-06 | Breeding method of Chinese cabbage Aikang No.4 |
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|---|---|---|---|
| CN98111283A CN1085039C (en) | 1998-05-06 | 1998-05-06 | Breeding method of Chinese cabbage Aikang No.4 |
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| CN1197593A CN1197593A (en) | 1998-11-04 |
| CN1085039C true CN1085039C (en) | 2002-05-22 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101094589B (en) * | 2004-12-08 | 2011-01-05 | 尼克森茨瓦恩有限责任公司 | Resistance to downy mildew of onion caused by the fungus peronospora destructor |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101338341B (en) * | 2008-06-16 | 2013-05-22 | 南京农业大学 | Process for marking high Vc content gene molecules of Chinese cabbage |
| CN103733767A (en) * | 2013-11-22 | 2014-04-23 | 柯正光 | Prevention and treatment method for Chinese cabbage downy mildew |
| CN106069008B (en) * | 2016-06-15 | 2019-07-02 | 苏州市农业科学院 | A kind of selection of high-quality red three-coloured amaranth new varieties |
| CN112301090A (en) * | 2020-11-03 | 2021-02-02 | 上海市农业科学院 | Identification method of downy mildew-resistant germplasm material of non-heading Chinese cabbage |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1079861A (en) * | 1992-06-05 | 1993-12-29 | 陕西省蔬菜研究所 | Male-sterile line breeding method for allo-plasm of Chinese cabbage |
| CN1125036A (en) * | 1995-09-28 | 1996-06-26 | 吉林省蔬菜花卉科学研究所 | Crossbreeding technology of Chinese cabbage |
-
1998
- 1998-05-06 CN CN98111283A patent/CN1085039C/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1079861A (en) * | 1992-06-05 | 1993-12-29 | 陕西省蔬菜研究所 | Male-sterile line breeding method for allo-plasm of Chinese cabbage |
| CN1125036A (en) * | 1995-09-28 | 1996-06-26 | 吉林省蔬菜花卉科学研究所 | Crossbreeding technology of Chinese cabbage |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101094589B (en) * | 2004-12-08 | 2011-01-05 | 尼克森茨瓦恩有限责任公司 | Resistance to downy mildew of onion caused by the fungus peronospora destructor |
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| CN1197593A (en) | 1998-11-04 |
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