CN108485953A - A kind of germ culturing device and the method for the test of buccal cigarette bacteriostasis property - Google Patents
A kind of germ culturing device and the method for the test of buccal cigarette bacteriostasis property Download PDFInfo
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- CN108485953A CN108485953A CN201810366818.3A CN201810366818A CN108485953A CN 108485953 A CN108485953 A CN 108485953A CN 201810366818 A CN201810366818 A CN 201810366818A CN 108485953 A CN108485953 A CN 108485953A
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- buccal cigarette
- babinet
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- bacteriostasis property
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- 235000019504 cigarettes Nutrition 0.000 title claims abstract description 48
- 238000012360 testing method Methods 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 17
- 238000012258 culturing Methods 0.000 title claims abstract description 15
- 241000894006 Bacteria Species 0.000 claims abstract description 14
- 230000003750 conditioning effect Effects 0.000 claims abstract description 13
- 239000007789 gas Substances 0.000 claims description 20
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 14
- 239000001301 oxygen Substances 0.000 claims description 14
- 229910052760 oxygen Inorganic materials 0.000 claims description 14
- 229920001817 Agar Polymers 0.000 claims description 12
- 241000191967 Staphylococcus aureus Species 0.000 claims description 12
- 239000008272 agar Substances 0.000 claims description 12
- 238000012113 quantitative test Methods 0.000 claims description 10
- 238000010790 dilution Methods 0.000 claims description 9
- 239000012895 dilution Substances 0.000 claims description 9
- 235000013372 meat Nutrition 0.000 claims description 7
- 235000014347 soups Nutrition 0.000 claims description 7
- 241000607142 Salmonella Species 0.000 claims description 5
- 241000607768 Shigella Species 0.000 claims description 5
- 238000001514 detection method Methods 0.000 claims description 5
- 239000002609 medium Substances 0.000 claims description 5
- 241000588724 Escherichia coli Species 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 2
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical group C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 claims 1
- 241001478240 Coccus Species 0.000 claims 1
- 235000009754 Vitis X bourquina Nutrition 0.000 claims 1
- 235000012333 Vitis X labruscana Nutrition 0.000 claims 1
- 240000006365 Vitis vinifera Species 0.000 claims 1
- 235000014787 Vitis vinifera Nutrition 0.000 claims 1
- 238000001727 in vivo Methods 0.000 abstract description 2
- 241000208125 Nicotiana Species 0.000 description 5
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 5
- 230000002401 inhibitory effect Effects 0.000 description 3
- 210000000214 mouth Anatomy 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 230000003068 static effect Effects 0.000 description 3
- 244000269722 Thea sinensis Species 0.000 description 2
- 238000009863 impact test Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 241000305071 Enterobacterales Species 0.000 description 1
- 208000002193 Pain Diseases 0.000 description 1
- 238000000889 atomisation Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000036760 body temperature Effects 0.000 description 1
- 125000003636 chemical group Chemical group 0.000 description 1
- 230000001055 chewing effect Effects 0.000 description 1
- 229940112822 chewing gum Drugs 0.000 description 1
- 235000015218 chewing gum Nutrition 0.000 description 1
- 238000002485 combustion reaction Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003571 electronic cigarette Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000011261 inert gas Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 235000019505 tobacco product Nutrition 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/20—Heat exchange systems, e.g. heat jackets or outer envelopes the heat transfer medium being a gas
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/10—Enterobacteria
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/14—Streptococcus; Staphylococcus
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/24—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- G01N2333/25—Shigella (G)
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/24—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- G01N2333/255—Salmonella (G)
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/305—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Micrococcaceae (F)
- G01N2333/31—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Micrococcaceae (F) from Staphylococcus (G)
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
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Abstract
A kind of method the present invention relates to germ culturing device and for the test of buccal cigarette bacteriostasis property, the device includes multiple independent case bodies, it is equipped with turntable in each babinet, turntable is equipped with multiple fixing grooves for culture dish holding, temperature conditioning unit and gas exchange units are equipped in babinet, rotor is equipped in culture dish, device is additionally provided with controller, is connect with the temperature conditioning unit of all babinets and gas exchange units；Buccal cigarette to be measured and bacterium are placed on case In vivo culture, to judge the bacteriostasis property of buccal cigarette.Compared with prior art, for the device that the present invention can be through the invention to being carried out at the same time the test of bacteriostasis property with a batch of buccal cigarette, testing efficiency is high；And automation control is carried out to temperature conditioning unit, gas exchange units by controller, manual labor's intensity is small；Meanwhile test qualitatively and quantitatively can be carried out to the bacteriostasis property of buccal cigarette, test result is more comprehensively.
Description
Technical field
The present invention relates to detection authentication techniques fields, and in particular to a kind of germ culturing device and is used for buccal cigarette biocidal property
The method that can be tested.
Background technology
Smokeless cigarette refers to the cigarette replacement class product for entering oral cavity in sucking process without combustion product gases, principal mode packet
It includes chewing tobacco, buccal cigarette, snuff, chewing gum cigarette and battery driven electronic cigarette, or burning and generates the atomization production of thermal energy conduction heat
A kind of such as tobacco product of fog shape steam；Smokeless cigarette has without burning pipe tobacco and correlated product, without smog, chemical group
Divide controllable feature, it is more safer than regular-size cigarette, it is easy to carry, meet in certain group of people or specific occasion the needs of to cigarette,
And show a kind of cigarette consumption mode of individual character.
Buccal cigarette occupies an important position in smokeless cigarette, is a kind of smokeless tobacco articles originated in from Sweden.It is different from
Grandfather generation wipes the tobacco that on gums or need to be chewed, and in the pouch as tea bag, pouch can be placed on by user for it
Between upper lip and gums for 30 minutes, and it is not necessarily to scum.Buccal cigarette is the moistening tobacco by fine gtinding, in bulk or
Similar to the small packed form sale of Mini type tea bag.It is placed between upper lip and gum when use, without stinging without mill, one
As abandon after 30 minutes.
It since buccal cigarette need to stop in oral cavity, and is moistened in oral cavity, and proper temperature, a large amount of bacteriums may be grown,
To do harm to huamn body.Therefore, the bacteriostasis property of buccal cigarette becomes a major criterion for judging buccal cigarette quality.To same
A batch of buccal cigarette is detected, and existing method efficiency is very low.
Invention content
It is an object of the present invention to overcome the above-mentioned drawbacks of the prior art and provide a kind of germ culturing devices
And the method for the test of buccal cigarette bacteriostasis property.
The purpose of the present invention can be achieved through the following technical solutions：A kind of germ culturing device, the device include more
A independent case body, each babinet is interior to be equipped with turntable, and the turntable is equipped with multiple fixing grooves for culture dish holding, the case
It is equipped with temperature conditioning unit and gas exchange units in vivo, is equipped with rotor in culture dish, described device is additionally provided with controller, with all casees
The temperature conditioning unit and gas exchange units of body connect.It, will since the present apparatus is tested for the buccal cigarette of a batch
The Bacteria Culture that the same terms are carried out in the same babinet is placed on a batch of multiple samples to be tested, so in order to carry significantly
High testing efficiency.Simultaneously as culture dish is arranged on turntable, by the rotation of turntable, ensure the culture of all culture dishes
Identical when condition, when avoiding static gas wave refrigerator, the difference of certain conditions causes result inaccurate, when such as static gas wave refrigerator, some
Culture dish is closer to temperature conditioning unit so that the temperature of the culture dish is different from other culture dishes.It, can be outside the enclosure in order to distinguish
Screen is arranged in portion, shows the information such as producer, batch, the test strain of buccal cigarette.
Circumference on the turntable is equipped with equally distributed at least ten fixing groove.
The temperature conditioning unit includes the temperature sensor and thermoregulator being connect with controller.Due to different bacterium
The most suitable cultivation temperature of kind is slightly different, it is possible to by controller set temperature lower limit and temperature upper limit, when temperature passes
When sensor detects case body temperature higher than the upper limit or less than lower limit, signal is passed to controller, controller by temperature sensor
Control thermoregulator cooling or heating.
The babinet be equipped with air inlet and gas outlet, the gas exchange units include oxygen content detection sensor,
The air pump that is connect with air inlet and air valve in gas outlet is set, the oxygen level sensor, air pump, that is, air valve and controller
Connection.Since the different most suitable oxygen contents of strain is different, and the variation of oxygen content can be caused when Spawn incubation, it is possible to
The upper and lower bound of oxygen content is set by controller, when oxygen level sensor detects that oxygen content is less than lower limit in babinet,
Controller controls air pump and fresh sterile air is squeezed into babinet, while controller control is opened air valve and is exhausted；Work as oxygen content
When sensor detects that oxygen content is higher than the upper limit in babinet, fresh sterile inert gas is squeezed into babinet by controller control air pump,
Controller control simultaneously is opened air valve and is exhausted.
The controller is PLC controller.
In addition, being equipped with high-temperature steam nozzle and ultraviolet lamp in the babinet, the high-temperature steam nozzle connects with steam oven
It connects.High-temperature steam nozzle and ultraviolet lamp are set, is avoided to be sterilized before the test begins to babinet and culture dish
Miscellaneous bacteria has an impact test result.In test process, high-temperature steam nozzle and ultraviolet lamp do not work.
4 independent case bodies are included at least in described device.
A method of carrying out buccal cigarette bacteriostasis property test, including following step using germ culturing device as described above
Suddenly：
(1) at least 10 parts of conduct buccal cigarettes to be measured are randomly selected in a batch of buccal cigarette, and are crushed, with nothing
Bacterium DE meat soups are configured to dilution, for use；
(2) it takes the dilution of each buccal cigarette to be measured to be placed in culture dish, certain amount of quantitative test is then added
Strain takes 1mL to be placed in culture dish and agar is added, and the culture dish that quantitative test flora of the same race is added then is put into the same case
In body, whether after cultivating 48h, detect in each culture dish has change before quantitative test strain and culture；
(3) it takes the dilution of each buccal cigarette to be measured to be placed in container, DE meat soups is then added, certain number is then added
The qualitative test strain of amount is sucked out after mixing, and is cultivated in special media, in specific fine jade after 24~72h
It crosses on fat culture medium, then the culture medium with homospecificity agar plates is put into the same babinet, cultivate 48h
Whether occurs specific qualitative test strain afterwards.
The quantitative test strain includes the mixed bacteria or yeast and mold of Escherichia coli and staphylococcus aureus
Mixed bacteria, the quantity for the quantitative test strain being added in each culture dish is more than 105It is a.
The qualitative test strain includes Staphylococcus aureus, salmonella and Shigella, the specific agar
Culture medium is the agar medium of Staphylococcus aureus, salmonella and Shigella, the quantity of the qualitative test strain of addition
It is 80~150.
Compared with prior art, beneficial effects of the present invention are embodied in following several respects：
(1) device that can be through the invention is to being carried out at the same time the test of bacteriostasis property, test with a batch of buccal cigarette
It is efficient；
(2) automation control is carried out to temperature conditioning unit, gas exchange units by controller, manual labor's intensity is small；
(3) test qualitatively and quantitatively can be carried out to the bacteriostasis property of buccal cigarette, test result is more comprehensively.
Description of the drawings
Fig. 1 is the main structure diagram of each babinet in the present invention.
Wherein, 1 is babinet, and 2 be turntable, and 3 be culture dish, and 4 be rotor, and 5 be temperature sensor, and 6 be thermoregulator, 7
It is air pump for oxygen level sensor, 8,9 be air valve, and 10 be air inlet, and 11 be gas outlet, and 12 be fixing groove, and 13 be high-temperature steam
Nozzle, 14 be ultraviolet lamp.
Specific implementation mode
It elaborates below to the embodiment of the present invention, the present embodiment is carried out lower based on the technical solution of the present invention
Implement, gives detailed embodiment and specific operating process, but protection scope of the present invention is not limited to following implementation
Example.
Embodiment 1
A kind of germ culturing device, the device include six independent case bodies, and the structure of each babinet 1 is as shown in Figure 1, in it
The pedestal in portion is equipped with turntable 2, and turntable 2 is equipped with 10 fixing grooves 12 for being used for culture dish holding 3, temperature control is equipped in babinet 1
Unit and gas exchange units, culture dish 3 is interior to be equipped with rotor 4, and device is additionally provided with controller, the temperature conditioning unit with all babinets 1
And gas exchange units connection.Wherein, temperature conditioning unit includes the temperature sensor 5 being connect with controller and thermoregulator 6.
Babinet 1 is equipped with air inlet 10 and gas outlet 11, and gas exchange units include oxygen content detection sensor, connect with air inlet 10
Air pump 8 and air valve 9 in gas outlet 11 is set, oxygen level sensor 7, air pump 8 are that air valve 9 is connect with controller.
Since the present apparatus is tested for the buccal cigarette of a batch, will be put with a batch of multiple samples to be tested
The Bacteria Culture that the same terms are carried out in the same babinet 1 is set, so in order to substantially increase testing efficiency.Simultaneously as
Culture dish 3 is arranged on turntable 2, by the rotation of turntable 2, ensures identical when the condition of culture of all culture dishes 3, avoids quiet
When only cultivating, the difference of certain conditions causes result inaccurate, and when such as static gas wave refrigerator, some culture dish 3 is closer to temperature control list
Member so that the temperature of the culture dish 3 is different from other culture dishes 3.In order to distinguish, screen can be set outside babinet 1, shown
The information such as producer, batch, the test strain of buccal cigarette.
High-temperature steam nozzle 13 and ultraviolet lamp 14 are equipped in babinet 1, high-temperature steam nozzle 13 is connect with steam oven.Setting
High-temperature steam nozzle 13 and ultraviolet lamp 14 are to sterilize, avoid to babinet 1 and culture dish 3 before the test begins
Miscellaneous bacteria has an impact test result, in test process, high-temperature steam spray, 13 and ultraviolet lamp 14 do not work.
Concrete operation step is as follows：
(1) culture dish is placed in babinet, opens ultraviolet lamp and high-temperature steam nozzle, degerming is carried out to box house,
Stop after 1h, waits for that babinet cools down；
(2) 10 samples in buccal cigarette to be measured are randomly selected, are dispersed in after crushing in DE meat soups, be configured to 1~No. 10 it is dilute
Release liquid；
(3) then 120000 staphylococcus aureuses and Escherichia coli in total are added in 1~No. 10 dilution for taking equivalent
Mixed bacteria liquid, the DE meat soups and agar of equivalent is then added, each dilution 1mL after being diluted is added to sterile petri dish
In, culture dish is put into 10 fixing grooves of turntable, 48h is cultivated in babinet, it is big in 10 culture mediums before and after detection culture
Whether the quantity of enterobacteria and staphylococcus aureus changes, to judge the buccal cigarette to Escherichia coli and Staphylococcus aureus
The inhibiting effect of bacterium；
(4) using the identical method with step (3), this batch of buccal cigarette is tested respectively, the inhibition of yeast and mold is made
With；
(5) it takes 1~No. 10 dilution of equivalent to be placed in 10 containers through sterilization, is then added in each container
DE meat soups and 100 Staphylococcus aureus, are sucked out part solution, and in the special media of Staphylococcus aureus immediately after
Upper culture for 24 hours, is then crossed on specific agar medium, then by the training with scribed specific agar medium
Foster ware is put into babinet, after cultivating 48h, has seen whether that Staphylococcus aureus bacterium colony occurs, to judge this batch of buccal cigarette
To the instant inhibiting effect of Staphylococcus aureus；
(6) using the identical method with step (5), this batch of buccal cigarette is tested respectively to salmonella and Shigella i.e.
When inhibiting effect；
(7) in summary experimental result, obtains the bacteriostasis property of this batch of buccal cigarette.
Claims (9)
1. a kind of germ culturing device, which is characterized in that the device includes multiple independent case bodies, and turntable is equipped in each babinet,
The turntable is equipped with multiple fixing grooves for culture dish holding, and temperature conditioning unit and gas exchanges list are equipped in the babinet
Member, culture dish is interior to be equipped with rotor, and described device is additionally provided with controller, connects with the temperature conditioning unit and gas exchange units of all babinets
It connects.
2. a kind of germ culturing device according to claim 1, which is characterized in that the circumference on the turntable is equipped with
Equally distributed at least ten fixing groove.
3. a kind of germ culturing device according to claim 1, which is characterized in that the temperature conditioning unit includes and control
The temperature sensor and thermoregulator of device connection.
4. a kind of germ culturing device according to claim 1, which is characterized in that the babinet is equipped with air inlet and goes out
Gas port, the gas exchange units include oxygen content detection sensor, the air pump being connect with air inlet and are arranged in outlet
The air valve of mouth, the oxygen level sensor, air pump, that is, air valve are connect with controller.
5. a kind of germ culturing device according to claim 1, which is characterized in that the controller is PLC controller.
6. a kind of germ culturing device according to claim 1, which is characterized in that include at least 4 in described device solely
Vertical babinet.
7. a kind of method carrying out buccal cigarette bacteriostasis property test using the germ culturing device as described in claim 1~6 is any,
It is characterized by comprising the following steps：
(1) at least 10 parts of conduct buccal cigarettes to be measured are randomly selected in a batch of buccal cigarette, and are crushed, with sterile DE
Meat soup is configured to dilution, for use；
(2) it takes the dilution of each buccal cigarette to be measured to be placed in culture dish, certain amount of quantitative test strain is then added,
It takes 1mL to be placed in culture dish and agar is added, then the culture dish that quantitative test flora of the same race is added is put into the same babinet,
Whether after cultivating 48h, detect in each culture dish has change before quantitative test strain and culture；
(3) it takes the dilution of each buccal cigarette to be measured to be placed in container, DE meat soups is then added, are then added certain amount of
Qualitative test strain is sucked out after mixing, and is cultivated in special media, is trained in specific agar after 24~72h
It supports and crosses on base, then the culture medium with homospecificity agar plates is put into the same babinet, be after cultivating 48h
The specific qualitative test strain of no appearance.
8. a kind of method for the test of buccal cigarette bacteriostasis property according to claim 7, which is characterized in that described quantitative
Test strain includes the mixed bacteria of Escherichia coli and staphylococcus aureus or the mixed bacteria of yeast and mold, each
The quantity for the quantitative test strain being added in culture dish is more than 105It is a.
9. a kind of method for the test of buccal cigarette bacteriostasis property according to claim 7, which is characterized in that described qualitative
Test strain includes Staphylococcus aureus, salmonella and Shigella, and the specific agar medium is golden yellow grape
The quantity of the agar medium of coccus, salmonella and Shigella, the qualitative test strain of addition is 80~150.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810366818.3A CN108485953B (en) | 2018-04-23 | 2018-04-23 | Bacteria culture device and method for testing antibacterial performance of buccal cigarettes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810366818.3A CN108485953B (en) | 2018-04-23 | 2018-04-23 | Bacteria culture device and method for testing antibacterial performance of buccal cigarettes |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108485953A true CN108485953A (en) | 2018-09-04 |
| CN108485953B CN108485953B (en) | 2023-08-01 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111057739A (en) * | 2018-10-17 | 2020-04-24 | 上海新型烟草制品研究院有限公司 | Method for detecting coliform group in buccal cigarette |
Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2282550Y (en) * | 1997-04-08 | 1998-05-27 | 孙德明 | Air bacteria state indicator |
| US20020045592A1 (en) * | 1999-01-27 | 2002-04-18 | Elitra Pharmaceuticals, Inc. | Genes identified as required for proliferation in escherichia coli |
| RU2410437C1 (en) * | 2009-11-24 | 2011-01-27 | Ляля Степановна Тирранен | Method of determining bacteriostatic, bactericidal and stimulating effect of antibiotic on microorganism |
| CN102433373A (en) * | 2011-12-14 | 2012-05-02 | 浙江省农业科学院 | Salmonella characteristic chromogenic liquid nutrient medium, preparation method thereof and rapid detection method of salmonella |
| CN104762365A (en) * | 2015-04-22 | 2015-07-08 | 云南中烟工业有限责任公司 | Detection method for bacterium reverse mutation of buccal tobacco product |
| CN104774768A (en) * | 2014-12-30 | 2015-07-15 | 四川农业大学 | Fritillaria wabuensis endophytic fungi WBS003 and application thereof |
| CN104789633A (en) * | 2015-04-22 | 2015-07-22 | 云南中烟工业有限责任公司 | Test method for detecting cytotoxicity of buccal tobacco products |
| CN104894209A (en) * | 2015-04-22 | 2015-09-09 | 云南中烟工业有限责任公司 | Safety comparison and detection method for snus products |
| CN105029673A (en) * | 2015-07-23 | 2015-11-11 | 川渝中烟工业有限责任公司 | Buccal cigarette products containing probiotics |
| CN106472572A (en) * | 2016-10-10 | 2017-03-08 | 武汉工程大学 | A kind of microbial bactericide and preparation method and application |
| CN107619856A (en) * | 2017-10-21 | 2018-01-23 | 云南中烟工业有限责任公司 | A kind of detection method of buccal tobacco product to the total bacteria effect of saliva |
| WO2018018784A1 (en) * | 2016-07-26 | 2018-02-01 | 紫罗兰家纺科技股份有限公司 | Method for testing antibacterial performance of probiotic antibacterial home textile |
| CN206970580U (en) * | 2017-04-06 | 2018-02-06 | 浙江中医药大学附属第二医院 | A kind of novel bacterial culture experiment device |
-
2018
- 2018-04-23 CN CN201810366818.3A patent/CN108485953B/en active Active
Patent Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2282550Y (en) * | 1997-04-08 | 1998-05-27 | 孙德明 | Air bacteria state indicator |
| US20020045592A1 (en) * | 1999-01-27 | 2002-04-18 | Elitra Pharmaceuticals, Inc. | Genes identified as required for proliferation in escherichia coli |
| RU2410437C1 (en) * | 2009-11-24 | 2011-01-27 | Ляля Степановна Тирранен | Method of determining bacteriostatic, bactericidal and stimulating effect of antibiotic on microorganism |
| CN102433373A (en) * | 2011-12-14 | 2012-05-02 | 浙江省农业科学院 | Salmonella characteristic chromogenic liquid nutrient medium, preparation method thereof and rapid detection method of salmonella |
| CN104774768A (en) * | 2014-12-30 | 2015-07-15 | 四川农业大学 | Fritillaria wabuensis endophytic fungi WBS003 and application thereof |
| CN104789633A (en) * | 2015-04-22 | 2015-07-22 | 云南中烟工业有限责任公司 | Test method for detecting cytotoxicity of buccal tobacco products |
| CN104762365A (en) * | 2015-04-22 | 2015-07-08 | 云南中烟工业有限责任公司 | Detection method for bacterium reverse mutation of buccal tobacco product |
| CN104894209A (en) * | 2015-04-22 | 2015-09-09 | 云南中烟工业有限责任公司 | Safety comparison and detection method for snus products |
| CN105029673A (en) * | 2015-07-23 | 2015-11-11 | 川渝中烟工业有限责任公司 | Buccal cigarette products containing probiotics |
| WO2018018784A1 (en) * | 2016-07-26 | 2018-02-01 | 紫罗兰家纺科技股份有限公司 | Method for testing antibacterial performance of probiotic antibacterial home textile |
| CN106472572A (en) * | 2016-10-10 | 2017-03-08 | 武汉工程大学 | A kind of microbial bactericide and preparation method and application |
| CN206970580U (en) * | 2017-04-06 | 2018-02-06 | 浙江中医药大学附属第二医院 | A kind of novel bacterial culture experiment device |
| CN107619856A (en) * | 2017-10-21 | 2018-01-23 | 云南中烟工业有限责任公司 | A kind of detection method of buccal tobacco product to the total bacteria effect of saliva |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111057739A (en) * | 2018-10-17 | 2020-04-24 | 上海新型烟草制品研究院有限公司 | Method for detecting coliform group in buccal cigarette |
| CN111057739B (en) * | 2018-10-17 | 2023-11-21 | 上海新型烟草制品研究院有限公司 | Method for detecting coliform group bacteria in buccal cigarette |
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| CN108485953B (en) | 2023-08-01 |
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