CN108485282A - A kind of preparation method of environment-friendly type meat product packaging material - Google Patents
A kind of preparation method of environment-friendly type meat product packaging material Download PDFInfo
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- CN108485282A CN108485282A CN201810418447.9A CN201810418447A CN108485282A CN 108485282 A CN108485282 A CN 108485282A CN 201810418447 A CN201810418447 A CN 201810418447A CN 108485282 A CN108485282 A CN 108485282A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L89/00—Compositions of proteins; Compositions of derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L5/00—Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00
- C08L5/08—Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L2205/00—Polymer mixtures characterised by other features
- C08L2205/02—Polymer mixtures characterised by other features containing two or more polymers of the same C08L -group
- C08L2205/025—Polymer mixtures characterised by other features containing two or more polymers of the same C08L -group containing two or more polymers of the same hierarchy C08L, and differing only in parameters such as density, comonomer content, molecular weight, structure
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L2205/00—Polymer mixtures characterised by other features
- C08L2205/03—Polymer mixtures characterised by other features containing three or more polymers in a blend
- C08L2205/035—Polymer mixtures characterised by other features containing three or more polymers in a blend containing four or more polymers in a blend
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Abstract
The present invention provides a kind of preparation methods of environment-friendly type meat product packaging material, include the following steps：Silkworm Cocoon Shell fragment is taken, boiling is added to boil；Filtering removal impurity, concentration and evaporation obtain silk gum solution；The removing that chloroform is added in fresh porcine skin and absolute ethyl alcohol carries out fat, filtering is taken to add sodium carbonate and deionized water to remove fat again, cleaned after filtering；Sodium chloride and deionized water is added to stir；After microwave irradiation plus Tis HCl buffer solutions, protease extract；After enzyme deactivation, extracting solution is centrifuged, and supernatant is taken to be freeze-dried to obtain collagen；Shell adding glycan, silk gum solution, wax insect polysaccharide, ginger essential oil microballoon and acetic acid solution stirring, dialyse, freeze-drying；1 (3 dimethylamino-propyl) 3 ethyl carbodiimides and glutaraldehyde, stirring, washing to neutrality is added；Freezing；It is freeze-dried to obtain the final product.Packaging material prepared by this method has good mechanical property, and water imbibition is fine, while antibiotic property effect is also fine.
Description
Technical field
The present invention relates to a kind of preparation methods of environment-friendly type meat product packaging material.
Background technology
China is that the first big country of meat product production and consumption, meat product production and processing industry exist in the world at present
There is critical role in the world, but compared with developed country, China's meat products deep processing rate still has greater room for improvement.With
The development and the improvement of people's living standards of society, consumer are continuously increased meat products demand, and the quality of meat products is excellent
Bad and safety is of increased attention.Freshness is the direct reflection of the putrid and deteriorated degree of meat, is evaluation meat
The important indicator of Quality Safety.However meat products is usually easy that juice is precipitated when stored, and so that bacterium largely breeds, from
And the quality of meat products is destroyed, shorten the shelf-life, if the packaging material of meat products can effectively absorb juice, while again
With antibacterial functions, then, for enhancing the fresh-keeping effect of meat products, extends the shelf life, all have great importance.
Invention content
Technical problems to be solved：The object of the present invention is to provide a kind of preparations of environment-friendly type meat product packaging material
Method, prepared environment-friendly type meat product have good mechanical property with packaging material, and water imbibition is fine, while antibiotic property
Effect is also fine.
Technical solution：A kind of preparation method of environment-friendly type meat product packaging material, ingredient by weight, including with
Lower step：
(1) 50-70 parts of Silkworm Cocoon Shell fragments are taken, 200-300 parts of boilings are added and boil 30-40min；
(2) use cloth funnel filtering removal impurity, be put into rotary evaporator carry out be concentrated in vacuo evaporate 0.1g/mL silk gum is molten
Liquid；
(3) it takes 40-60 parts of fresh porcine skins to be cut into small pieces, 40-60 parts of chloroforms is added and 20-30 parts of absolute ethyl alcohols carry out fat
Removing, filtering, the solution for adding 4-5 parts of sodium carbonate and 50-65 parts of deionized waters removes fat, clear water removed after filtering again
Cleaning 2 times；
(4) 0.5-1 parts of sodium chloride and 90-120 parts of deionized waters are added, desalination dissolubility foreign protein is removed in stirring；
(5) carry out being added after microwave irradiation 1-5min at 20-30 DEG C of temperature 40-50 portions of Tis-HCl buffer solutions, 0.1-0.2 parts
Trypsase and 0.05-0.1 parts of papains extract 24-30h；
(6) after enzyme deactivation, extracting solution is centrifuged, and supernatant is taken to carry out being freeze-dried to obtain collagen；
(7) chitosan, silk gum solution, 0.2-0.3 parts of wax insect polysaccharide, the 0.5-1 parts of ginger extracts of the quality such as collagen are added
The acetic acid solution of oily microballoon and 70-100 parts 2%, magnetic agitation 6-7h dialyse for 24 hours in distilled water, freeze-drying；
(8) be added 70-100 parts of 50mM 1- (3- dimethylamino-propyls) -3- ethyl carbodiimides and 20-30 parts 0.25% penta
Dialdehyde is stirred to react 6-7h, is washed with distilled water to neutrality；
(9) it is transferred in refrigerator, 6-7h is freezed at -80 DEG C after freezing 6-7h at -20 DEG C；
(10) it is put into freeze drier vacuum freeze drying to obtain the final product.
1. a kind of preparation method of environment-friendly type meat product packaging material according to claim 1, it is characterised in that：Institute
State the fritter that pigskin is cut into 1cm × 1cm × 1cm.
2. a kind of preparation method of environment-friendly type meat product packaging material according to claim 1, it is characterised in that：Institute
State the preparation method of ginger essential oil microballoon：
The first step：2-5 parts of ethyl celluloses are taken, 80% ethyl alcohol of 40-60 parts, stirring and dissolving is added；
Second step：5-10 parts of ginger essential oils and 2% glycerine are added, stir；
Third walks：1wt% talcum powder is added, is stirred evenly；
4th step：It is spray-dried to obtain the final product, charging rate 3mL/min, inlet air temperature is 100 DEG C, atomizing pressure 100kPa.
Advantageous effect：
1. chitosan, ginger essential oil are all effectively antimicrobial components, can effective antibacterial, it is fresh-keeping, improve and be packaged ingredient
Storage time.
2. chitosan is capable of providing the more hydrophilic radicals of amino group more required than chemical crosslinking, so as to cause crosslinking
The reduction of degree, water imbibition increased.
3. there is packaging material of the present invention good mechanical property, tensile strength to be up to 18.62MPa, swelling ratio reaches as high as
44.4%, water imbibition is fine, while antibiotic property is good, fine for the inhibition of Escherichia coli.
Specific implementation mode
Embodiment 1
A kind of preparation method of environment-friendly type meat product packaging material, ingredient by weight, include the following steps：
(1) 50 parts of Silkworm Cocoon Shell fragments are taken, 200 parts of boilings are added and boil 30min；
(2) use cloth funnel filtering removal impurity, be put into rotary evaporator carry out be concentrated in vacuo evaporate 0.1g/mL silk gum is molten
Liquid；
(3) it takes 40 parts of fresh porcine skins to be cut into small pieces, 40 parts of chloroforms is added and 20 parts of absolute ethyl alcohols carry out the removing of fat, mistake
Filter, the solution for adding 4 parts of sodium carbonate and 50 parts of deionized waters remove fat again, and clear water is gone to clean after filtering 2 times；
(4) 0.5 part of sodium chloride and 90 parts of deionized waters are added, desalination dissolubility foreign protein is removed in stirring；
(5) carry out being added after microwave irradiation 1min at 20 DEG C of temperature 40 portions of Tis-HCl buffer solutions, 0.1 part of trypsase and
0.05 part of papain extracts for 24 hours；
(6) after enzyme deactivation, extracting solution is centrifuged, and supernatant is taken to carry out being freeze-dried to obtain collagen；
(7) chitosan, silk gum solution, 0.2 part of wax insect polysaccharide, the 0.5 part of ginger essential oil microballoon of the quality such as collagen is added
With 70 part 2% of acetic acid solution, magnetic agitation 6h dialyses for 24 hours in distilled water, freeze-drying；
(8) 1- (3- the dimethylamino-propyls) -3- ethyl carbodiimides and 20 part 0.25% of glutaraldehyde of 70 parts of 50mM is added, stirs
Reaction 6h is mixed, neutrality is washed with distilled water to；
(9) it is transferred in refrigerator, 6h is freezed at -80 DEG C after freezing 6h at -20 DEG C；
(10) it is put into freeze drier vacuum freeze drying to obtain the final product.
Embodiment 2
A kind of preparation method of environment-friendly type meat product packaging material, ingredient by weight, include the following steps：
(1) 60 parts of Silkworm Cocoon Shell fragments are taken, 250 parts of boilings are added and boil 35min；
(2) use cloth funnel filtering removal impurity, be put into rotary evaporator carry out be concentrated in vacuo evaporate 0.1g/mL silk gum is molten
Liquid；
(3) it takes 50 parts of fresh porcine skins to be cut into small pieces, 50 parts of chloroforms is added and 25 parts of absolute ethyl alcohols carry out the removing of fat, mistake
Filter, the solution for adding 4.5 parts of sodium carbonate and 60 parts of deionized waters remove fat again, and clear water is gone to clean after filtering 2 times；
(4) 0.8 part of sodium chloride and 110 parts of deionized waters are added, desalination dissolubility foreign protein is removed in stirring；
(5) carry out being added after microwave irradiation 4min at 25 DEG C of temperature 45 portions of Tis-HCl buffer solutions, 0.15 part of trypsase and
0.08 part of papain extracts 27h；
(6) after enzyme deactivation, extracting solution is centrifuged, and supernatant is taken to carry out being freeze-dried to obtain collagen；
(7) chitosan, silk gum solution, 0.25 part of wax insect polysaccharide, the 0.8 part of ginger essential oil microballoon of the quality such as collagen is added
With 90 part 2% of acetic acid solution, magnetic agitation 6.5h dialyses for 24 hours in distilled water, freeze-drying；
(8) 1- (3- the dimethylamino-propyls) -3- ethyl carbodiimides and 25 part 0.25% of glutaraldehyde of 90 parts of 50mM is added, stirs
Reaction 6.5h is mixed, neutrality is washed with distilled water to；
(9) it is transferred in refrigerator, 6.5h is freezed at -80 DEG C after freezing 6.5h at -20 DEG C；
(10) it is put into freeze drier vacuum freeze drying to obtain the final product.
Embodiment 3
A kind of preparation method of environment-friendly type meat product packaging material, ingredient by weight, include the following steps：
(1) 70 parts of Silkworm Cocoon Shell fragments are taken, 300 parts of boilings are added and boil 40min；
(2) use cloth funnel filtering removal impurity, be put into rotary evaporator carry out be concentrated in vacuo evaporate 0.1g/mL silk gum is molten
Liquid；
(3) it takes 60 parts of fresh porcine skins to be cut into small pieces, 60 parts of chloroforms is added and 30 parts of absolute ethyl alcohols carry out the removing of fat, mistake
Filter, the solution for adding 5 parts of sodium carbonate and 65 parts of deionized waters remove fat again, and clear water is gone to clean after filtering 2 times；
(4) 1 part of sodium chloride and 120 parts of deionized waters are added, desalination dissolubility foreign protein is removed in stirring；
(5) 50 portions of Tis-HCl buffer solutions, 0.2 part of trypsase and 0.1 is added after carrying out microwave irradiation 5min at 30 DEG C of temperature
Part papain extracts 30h；
(6) after enzyme deactivation, extracting solution is centrifuged, and supernatant is taken to carry out being freeze-dried to obtain collagen；
(7) be added the chitosans of the quality such as collagen, silk gum solution, 0.3 part of wax insect polysaccharide, 1 part of ginger essential oil microballoon and
100 part 2% of acetic acid solution, magnetic agitation 7h dialyse for 24 hours in distilled water, freeze-drying；
(8) 1- (3- the dimethylamino-propyls) -3- ethyl carbodiimides and 30 part 0.25% of glutaraldehyde of 100 parts of 50mM is added,
It is stirred to react 7h, is washed with distilled water to neutrality；
(9) it is transferred in refrigerator, 7h is freezed at -80 DEG C after freezing 7h at -20 DEG C；
(10) it is put into freeze drier vacuum freeze drying to obtain the final product.
Comparative example 1
The present embodiment is with embodiment 1 the difference is that replacing collagen with silk gum solution, specially：
A kind of preparation method of environment-friendly type meat product packaging material, ingredient by weight, include the following steps：
(1) 100 parts of Silkworm Cocoon Shell fragments are taken, 200 parts of boilings are added and boil 30min；
(2) use cloth funnel filtering removal impurity, be put into rotary evaporator carry out be concentrated in vacuo evaporate 0.1g/mL silk gum is molten
Liquid；
(3) chitosan of silk gum solution half quality, 0.2 part of wax insect polysaccharide, 0.5 part of ginger essential oil microballoon and 70 parts are added
2% acetic acid solution, magnetic agitation 6h dialyse for 24 hours in distilled water, freeze-drying；
(4) 1- (3- the dimethylamino-propyls) -3- ethyl carbodiimides and 20 part 0.25% of glutaraldehyde of 70 parts of 50mM is added, stirs
Reaction 6h is mixed, neutrality is washed with distilled water to；
(5) it is transferred in refrigerator, 6h is freezed at -80 DEG C after freezing 6h at -20 DEG C；
(6) it is put into freeze drier vacuum freeze drying to obtain the final product.
Comparative example 2
The present embodiment is with embodiment 1 the difference is that collagen replaces silk gum solution, specially：
A kind of preparation method of environment-friendly type meat product packaging material, ingredient by weight, include the following steps：
(1) it takes 80 parts of fresh porcine skins to be cut into small pieces, 80 parts of chloroforms is added and 40 parts of absolute ethyl alcohols carry out the removing of fat, mistake
Filter, the solution for adding 8 parts of sodium carbonate and 100 parts of deionized waters remove fat again, and clear water is gone to clean after filtering 2 times；
(2) 1 part of sodium chloride and 180 parts of deionized waters are added, desalination dissolubility foreign protein is removed in stirring；
(3) 80 portions of Tis-HCl buffer solutions, 0.2 part of trypsase and 0.1 is added after carrying out microwave irradiation 1min at 20 DEG C of temperature
Part papain extracts for 24 hours；
(4) after enzyme deactivation, extracting solution is centrifuged, and supernatant is taken to carry out being freeze-dried to obtain collagen；
(5) chitosan of collagen half quality, 0.2 part of wax insect polysaccharide, 0.5 part of ginger essential oil microballoon and 70 parts are added
2% acetic acid solution, magnetic agitation 6h dialyse for 24 hours in distilled water, freeze-drying；
(6) 1- (3- the dimethylamino-propyls) -3- ethyl carbodiimides and 20 part 0.25% of glutaraldehyde of 70 parts of 50mM is added, stirs
Reaction 6h is mixed, neutrality is washed with distilled water to；
(7) it is transferred in refrigerator, 6h is freezed at -80 DEG C after freezing 6h at -20 DEG C；
(8) it is put into freeze drier vacuum freeze drying to obtain the final product.
Comparative example 3
The present embodiment and embodiment 1 the difference is that replace glutaraldehyde with 1- (3- dimethylamino-propyls) -3- ethyl carbodiimides,
Specially：
A kind of preparation method of environment-friendly type meat product packaging material, ingredient by weight, include the following steps：
(1) 50 parts of Silkworm Cocoon Shell fragments are taken, 200 parts of boilings are added and boil 30min；
(2) use cloth funnel filtering removal impurity, be put into rotary evaporator carry out be concentrated in vacuo evaporate 0.1g/mL silk gum is molten
Liquid；
(3) it takes 40 parts of fresh porcine skins to be cut into small pieces, 40 parts of chloroforms is added and 20 parts of absolute ethyl alcohols carry out the removing of fat, mistake
Filter, the solution for adding 4 parts of sodium carbonate and 50 parts of deionized waters remove fat again, and clear water is gone to clean after filtering 2 times；
(4) 0.5 part of sodium chloride and 90 parts of deionized waters are added, desalination dissolubility foreign protein is removed in stirring；
(5) carry out being added after microwave irradiation 1min at 20 DEG C of temperature 40 portions of Tis-HCl buffer solutions, 0.1 part of trypsase and
0.05 part of papain extracts for 24 hours；
(6) after enzyme deactivation, extracting solution is centrifuged, and supernatant is taken to carry out being freeze-dried to obtain collagen；
(7) chitosan, silk gum solution, 0.2 part of wax insect polysaccharide, the 0.5 part of ginger essential oil microballoon of the quality such as collagen is added
With 70 part 2% of acetic acid solution, magnetic agitation 6h dialyses for 24 hours in distilled water, freeze-drying；
(8) 1- (3- dimethylamino-propyls) -3- ethyl carbodiimides of 90 parts of 50mM are added, are stirred to react 6h, are washed with distillation
It washs to neutrality；
(9) it is transferred in refrigerator, 6h is freezed at -80 DEG C after freezing 6h at -20 DEG C；
(10) it is put into freeze drier vacuum freeze drying to obtain the final product.
The measurement of biocidal property：Packaging film is measured to the inhibition for trying bacterium using filter paper inhibition zone method, will be accessed for examination bacterium
In aseptic liquid nutrient medium, in 37 DEG C, the constant-temperature table of 120r/min activation cultivate for 24 hours.Draw the bacterium after part activation
In kind liquid access sterile water, fully 10 are made after oscillation7The initial bacterium solution of cfu/mL.By sterilized LB solid mediums in room
Temperature is lower to place, and is cooled to after preference temperature down to sterile petri dish, spare after culture medium solidification.Draw the initial bacterium solutions of 0.1mL
LB culture mediums are seeded to, bacterium solution is uniformly dispersed with sterilized glass spreading rod.1g wrapping papers are taken to be dissolved in 10ml deionized waters
In, it takes 10uL wrapping papers drop in the aseptic filter paper on piece of a diameter of 6mm, is dripped filter paper in superclean bench with tweezers
It is dry, the filter paper of sterile water is added dropwise as a contrast.Then it is affixed on containing on the culture medium for trying bacterium, tablet back-off is positioned over temperature
In the incubator that degree is 37 DEG C for 24 hours.After culture, antibacterial circle diameter is measured using vernier caliper.
The partial properties of 1 environment-friendly type meat product packaging material of table
Environment-friendly type meat product prepared by the method for the present invention with packaging material there is good mechanical property, tensile strength to be up to
18.62MPa, swelling ratio reach as high as 44.4%, and water imbibition is fine, while antibiotic property is good, for the inhibition of Escherichia coli
Very well.
Claims (3)
1. a kind of preparation method of environment-friendly type meat product packaging material, it is characterised in that：Ingredient by weight, including with
Lower step：
(1) 50-70 parts of Silkworm Cocoon Shell fragments are taken, 200-300 parts of boilings are added and boil 30-40min；
(2) use cloth funnel filtering removal impurity, be put into rotary evaporator carry out be concentrated in vacuo evaporate 0.1g/mL silk gum is molten
Liquid；
(3) it takes 40-60 parts of fresh porcine skins to be cut into small pieces, 40-60 parts of chloroforms is added and 20-30 parts of absolute ethyl alcohols carry out fat
Removing, filtering, the solution for adding 4-5 parts of sodium carbonate and 50-65 parts of deionized waters removes fat, clear water removed after filtering again
Cleaning 2 times；
(4) 0.5-1 parts of sodium chloride and 90-120 parts of deionized waters are added, desalination dissolubility foreign protein is removed in stirring；
(5) carry out being added after microwave irradiation 1-5min at 20-30 DEG C of temperature 40-50 portions of Tis-HCl buffer solutions, 0.1-0.2 parts
Trypsase and 0.05-0.1 parts of papains extract 24-30h；
(6) after enzyme deactivation, extracting solution is centrifuged, and supernatant is taken to carry out being freeze-dried to obtain collagen；
(7) chitosan, silk gum solution, 0.2-0.3 parts of wax insect polysaccharide, the 0.5-1 parts of ginger extracts of the quality such as collagen are added
The acetic acid solution of oily microballoon and 70-100 parts 2%, magnetic agitation 6-7h dialyse for 24 hours in distilled water, freeze-drying；
(8) be added 70-100 parts of 50mM 1- (3- dimethylamino-propyls) -3- ethyl carbodiimides and 20-30 parts 0.25% penta
Dialdehyde is stirred to react 6-7h, is washed with distilled water to neutrality；
(9) it is transferred in refrigerator, 6-7h is freezed at -80 DEG C after freezing 6-7h at -20 DEG C；
(10) it is put into freeze drier vacuum freeze drying to obtain the final product.
2. a kind of preparation method of environment-friendly type meat product packaging material according to claim 1, it is characterised in that：Institute
State the fritter that pigskin is cut into 1cm × 1cm × 1cm.
3. a kind of preparation method of environment-friendly type meat product packaging material according to claim 1, it is characterised in that：Institute
State the preparation method of ginger essential oil microballoon：
The first step：2-5 parts of ethyl celluloses are taken, 80% ethyl alcohol of 40-60 parts, stirring and dissolving is added；
Second step：5-10 parts of ginger essential oils and 2% glycerine are added, stir；
Third walks：1wt% talcum powder is added, is stirred evenly；
4th step：It is spray-dried to obtain the final product, charging rate 3mL/min, inlet air temperature is 100 DEG C, atomizing pressure 100kPa.
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| CN201810418447.9A CN108485282A (en) | 2018-05-03 | 2018-05-03 | A kind of preparation method of environment-friendly type meat product packaging material |
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| CN201810418447.9A CN108485282A (en) | 2018-05-03 | 2018-05-03 | A kind of preparation method of environment-friendly type meat product packaging material |
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Family
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109024087A (en) * | 2018-10-29 | 2018-12-18 | 浙江卫斯敦环境科技有限公司 | A kind of preparation method of methodof edible package paper |
| CN109385099A (en) * | 2018-10-29 | 2019-02-26 | 浙江卫斯敦环境科技有限公司 | A kind of preparation method of antibacterial hydroscopic packaging material |
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| CN102643554A (en) * | 2012-05-08 | 2012-08-22 | 福州大学 | Edible fishskin collagen antibiosis membrane and preparing method thereof |
| CN106750580A (en) * | 2017-01-09 | 2017-05-31 | 福州大学 | A kind of new edibility antibacterial food packaging film and preparation method thereof |
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2018
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| CN102643554A (en) * | 2012-05-08 | 2012-08-22 | 福州大学 | Edible fishskin collagen antibiosis membrane and preparing method thereof |
| CN106750580A (en) * | 2017-01-09 | 2017-05-31 | 福州大学 | A kind of new edibility antibacterial food packaging film and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109024087A (en) * | 2018-10-29 | 2018-12-18 | 浙江卫斯敦环境科技有限公司 | A kind of preparation method of methodof edible package paper |
| CN109385099A (en) * | 2018-10-29 | 2019-02-26 | 浙江卫斯敦环境科技有限公司 | A kind of preparation method of antibacterial hydroscopic packaging material |
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