CN109762060A - Collagen processing method - Google Patents
Collagen processing method Download PDFInfo
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- CN109762060A CN109762060A CN201810341596.XA CN201810341596A CN109762060A CN 109762060 A CN109762060 A CN 109762060A CN 201810341596 A CN201810341596 A CN 201810341596A CN 109762060 A CN109762060 A CN 109762060A
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- collagen
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- acid solution
- mixed liquor
- pigskin
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- 102000008186 Collagen Human genes 0.000 title claims abstract description 93
- 108010035532 Collagen Proteins 0.000 title claims abstract description 93
- 229920001436 collagen Polymers 0.000 title claims abstract description 93
- 238000003672 processing method Methods 0.000 title claims abstract description 36
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 34
- 239000000203 mixture Substances 0.000 claims abstract description 32
- 239000002253 acid Substances 0.000 claims abstract description 26
- 108091005804 Peptidases Proteins 0.000 claims abstract description 15
- 239000004365 Protease Substances 0.000 claims abstract description 15
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 15
- 238000004140 cleaning Methods 0.000 claims abstract description 9
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 45
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 18
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 8
- 239000012530 fluid Substances 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 7
- 239000004519 grease Substances 0.000 claims description 6
- 102000004190 Enzymes Human genes 0.000 claims description 5
- 108090000790 Enzymes Proteins 0.000 claims description 5
- 239000011780 sodium chloride Substances 0.000 claims description 5
- 229910000403 monosodium phosphate Inorganic materials 0.000 claims description 4
- 235000019799 monosodium phosphate Nutrition 0.000 claims description 4
- 235000017557 sodium bicarbonate Nutrition 0.000 claims description 4
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 4
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 claims description 4
- 239000012535 impurity Substances 0.000 claims description 3
- 239000003292 glue Substances 0.000 claims description 2
- 230000001954 sterilising effect Effects 0.000 claims description 2
- GCNLQHANGFOQKY-UHFFFAOYSA-N [C+4].[O-2].[O-2].[Ti+4] Chemical compound [C+4].[O-2].[O-2].[Ti+4] GCNLQHANGFOQKY-UHFFFAOYSA-N 0.000 claims 1
- 210000002318 cardia Anatomy 0.000 claims 1
- 210000002784 stomach Anatomy 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 7
- 238000011161 development Methods 0.000 abstract description 4
- 239000000284 extract Substances 0.000 abstract description 4
- 238000012545 processing Methods 0.000 abstract description 3
- 239000000243 solution Substances 0.000 description 46
- 238000001035 drying Methods 0.000 description 10
- 238000000605 extraction Methods 0.000 description 10
- 238000007654 immersion Methods 0.000 description 6
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 4
- 102000057297 Pepsin A Human genes 0.000 description 4
- 108090000284 Pepsin A Proteins 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 229940111202 pepsin Drugs 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 239000013078 crystal Substances 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 235000005979 Citrus limon Nutrition 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 244000248349 Citrus limon Species 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 1
- 101710172711 Structural protein Proteins 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- -1 acetum Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000001925 catabolic effect Effects 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 235000021245 dietary protein Nutrition 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000002500 effect on skin Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 125000004435 hydrogen atom Chemical class [H]* 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 235000019645 odor Nutrition 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 229940116540 protein supplement Drugs 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000000194 supercritical-fluid extraction Methods 0.000 description 1
- 239000000052 vinegar Substances 0.000 description 1
- 235000021419 vinegar Nutrition 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
Landscapes
- Peptides Or Proteins (AREA)
- Cosmetics (AREA)
- Meat, Egg Or Seafood Products (AREA)
Abstract
A kind of collagen processing method, be first by pigskin cleaning, de-oiling, de-taste, sterilize after obtain coarse fodder, then the coarse fodder is mixed with protease, extracts and filter after being placed in the acid solution mixing that pH value is 2~3, to obtain mixed liquor.Adjust the mixed liquor pH value inactivate protease in the mixed liquor after, the dry mixed liquor is to obtain mixture.The mixture is soaked in saline solution, to obtain the collagen body formed by the mixture.Collagen processing method of the present invention provides a kind of method of brand-new processing collagen, can promote collagen industry development.
Description
Technical field
The present invention relates to a kind of purifying, the method for extraction material, more particularly to a kind of collagen being capable of handling in pigskin
The collagen processing method of albumen.
Background technique
Collagen is the structural protein matter that content is numerous in human body, accounts for 25% or more of human body protein total amount, main
It is present in connective tissue, or is present in human body in the form of cytoplasm.There are many purposes for collagen, such as food
Protein supplements are applied in cosmetics and skin care products, or applied to medical domain etc..Since collagen is used
Way is wide and value is high, therefore company has all put into the research and development field of the production of collagen, manufacture perhaps.Therefore, how to provide
A kind of manufacturing method of new collagen, to promote the development of collagen industry, be one be worth furtherd investigate and be subject to
The project of solution.
Summary of the invention
The purpose of the present invention is to provide a kind of collagen processing of at least one disadvantage that can overcome prior art
Method.
Collagen processing method of the present invention includes step A: providing clean pigskin as coarse fodder, the collagen
Processing method also includes step B: the coarse fodder being mixed with protease, is placed in acid solution after extracting and filters to obtain
Mixed liquor；Step C: adjust the mixed liquor pH value inactivate protease in the mixed liquor after, it is dry described mixed
Liquid is closed to obtain mixture；And step D: the mixture is soaked in saline solution, to obtain being formed by the mixture
Collagen body.
Collagen processing method of the present invention in Yu Suoshu step A, is to pigskin cleaning, de-oiling, de-tastes, kills
Bacterium is to obtain clean rough bark as coarse fodder.
Collagen processing method of the present invention is to be mixed using supercritical fluid with pigskin in Yu Suoshu step A
To slough the grease of pigskin and de-taste.
Collagen processing method of the present invention, the supercritical fluid are carbon dioxide, and are 30 DEG C in temperature
~35 DEG C and pressure mix under conditions of being 300bar~400bar with pigskin.
Collagen processing method of the present invention, the pH value of the acid solution are 2~3.
Collagen processing method of the present invention, the protease are pepsin, thermophile protein enzyme, bullet
Property protease or any combination above-mentioned.
Collagen processing method of the present invention, the acid solution are that hydrochloric acid solution, acetum, citric acid are molten
Liquid or any combination above-mentioned.
Collagen processing method of the present invention, the saline solution are sodium chloride solution, sodium bicarbonate solution, phosphorus
Acid dihydride sodium solution or any combination above-mentioned.
Collagen processing method of the present invention, in Yu Suoshu step B, the acid solution is citric acid solution.
Collagen processing method of the present invention, also comprising step E, the Yu Suoshu step E after the step D
In, it is that the collagen body is driven to move repeatedly in cleaning solution, to wash away the impurity in the collagen body, and obtains
Collagen.
Collagen processing method of the present invention is the mixed liquor that will be adjusted after pH value in Yu Suoshu step C
It is dried in container to obtain the mixture, is to pour into saline solution in the container, to obtain in Yu Suoshu step D
The collagen body formed by the mixture.
Collagen processing method of the present invention in Yu Suoshu step E, is cleaned repeatedly until the collagen
Electrical conductivity less than 10 μ S/m.
In the step A, the pigskin bought preferably first is cleaned, and removes the pig hair being attached on pigskin, then benefit
It is mixed with supercritical fluid with pigskin, the oil extraction in pigskin is come out, slough grease and remove pigskin peculiar smell.It is described super
Critical fluids are preferably carbon dioxide, and preferably at 30 DEG C~35 DEG C of temperature, under conditions of pressure is 300bar~400bar
It is mixed with pigskin and carries out supercritical extract.Supercritical extract is carried out under aforementioned condition, it can be more effectively by the grease in pigskin
Removing, and remove odors.Preferably, also sterilized after with supercritical fluid extraction with hydrogen peroxide, it is safe and healthy to ensure.
In the step B, it for example can be hydrochloric acid solution, vinegar that preferably the pH value of the acid solution, which is 2~3,
Any combination of acid solution, citric acid solution or previous solu.Wherein, the acid solution is preferably hydrochloric acid solution or lemon
Lemon acid solution.More preferably, the acid solution is citric acid solution.The acid solution preferably selects hydrochloric acid solution, and more preferably
The reason of selecting citric acid solution illustrates after holding.In the step B, the protease is pepsin, thermophilic egg
Any combination of white enzyme, elastoser or aforementioned ferment.Preferably, the protease is pepsin, because of energy
Effect is preferably played in the acidic environment of the acid solution, and suitably shears the position of collagen.
In the step C, the mixture is actually mainly by the dry institute's shape of collagen in the mixed liquor
At, and containing because of salt caused by acid-base neutralization.Wherein, hydrochloric acid solution or citric acid solution are used such as in the step B
It is extracted, then when the pH value for adjusting the mixed liquor is so that protease inactivates, will be avoided that collagen is directly analysed
Reunite out, and the preferable sheet of one layer of continuity or membranaceous and transparent or translucent can be formed after the mixed liquor is dry
The mixture.
In the step D, the mixture under the action of the saline solution, contained by collagen, will
Overlie one another closer, and structure becomes more sturdy.The saline solution for example can be sodium chloride solution, carbonic acid
Any combination of hydrogen sodium solution, sodium dihydrogen phosphate or previous solu.
The effect of collagen processing method, is: providing a kind of collagen that can be effectively treated in pigskin
Method can be made the collagen body using pigskin, and can clean purifying further the collagen of purity is high is made, and supply
Related collagen industry uses, and promotes the development of collagen industry.
Preferably, the acid solution is citric acid solution in the step B.It is that will adjust in the step C
The mixed liquor after pH value is poured into container and is dried to obtain the mixture.It is to fall saline solution in the step D
Enter in the container, to obtain the collagen formed by the mixture after drying.The collagen processing side
Method also includes the step E after the step D.In the step E, be drive the collagen in cleaning solution repeatedly
It is mobile, to wash away the impurity in the collagen body.Preferably, in the step E being cleaned repeatedly until the collagen
The electrical conductivity of albumen is less than 10 μ S/m, to obtain the collagen of high-purity.
Due in the step B, the acid solution is using citric acid, therefore formed in the step C
The mixture will be in continuity preferable sheet or membranaceous, multiple to handle the mixture with the step D with saline solution,
When can make to clean in the step E in continuous sheet or the membranaceous collagen body, being not easy to scatter is melted, to have
Reduce the advantages that losing and improve yield and reducing production cost.
Detailed description of the invention
Others feature and effect of the invention, will clearly be presented in the embodiment referring to schema, in which:
Fig. 1 is a flow chart of steps, illustrates one embodiment 1 of collagen processing method of the present invention；
Fig. 2 is a photo, illustrates that a respective mixed liquor is adjusted in a drying process step S3 in embodiment 1,4,5
State after whole pH value；
Fig. 3 is a photo, is illustrated in embodiment 1,4,5 respectively obtained one in the drying process step S3
The state of mixture；
Fig. 4 is a photo, illustrates Examples 1 to 3 respective obtained glue in the immersion saline solution step S4
Former proteosome；And
Fig. 5 is a photo, illustrates the Western experimental result of collagen obtained by embodiment 1,4,5.
Specific embodiment
" embodiment 1 "
One embodiment 1 of collagen processing method of the present invention includes a pretreatment steps S1, an acid solution extraction
Take step S2, a drying process step S3, an immersion saline solution step S4 and a cleaning purification step S5.
< pretreatment steps S1 >
The full skin of pig examined through production and marketing is bought, after striking off the grease and pig hair of the full skin of pig, cuts out the corium portion of the full skin of pig
Position, and it is broken to grind obtained pigskin after the dermal sites of the full skin of pig are air-dried.With Co 2 supercritical fluid, in about 35 DEG C of temperature
And extract that pigskin is broken under conditions of pressure about 350bar, by pigskin it is broken in grease and extracting substances with peculiar smell come out.
It is cleaned with clear water by pigskin extracted is broken, and after sterilizing with hydrogen peroxide, it is spare to obtain coarse fodder.
< acid solution extraction step S2 >
In the acid solution extraction step S2, be first take the coarse fodder of about 125g be added about 250g comprising citric acid
In the acid solution of (Citric Acid, CA), and enough pepsin conducts are added under conditions of to maintain pH value be about 2~3
Protease extraction.Namely in this
Embodiment 1, is that the citric acid solution for being about 2~3 with pH value is extracted, and is to maintain pH value about in extraction process
It is 2~3.Solid component is filtered out with sieve after extraction 72 hours, a mixed liquor can be obtained.
< is dried step S3 >
The mixed liquor is poured into a container, and the sodium hydroxide solution about 900ml of concentration 5N is added described will mix
It closes liquid and is adjusted to alkaline (pH value about 8.5), and inactivate protease.The mixed liquor after adjusting pH value, will be such as the right side Fig. 2
It is shown, it is in as clear as crystal shape.The mixed liquor after dry adjustment pH value leaves accumulation in the appearance to remove liquid component
One mixture of device bottom is as shown in the right side Fig. 3.The mixture is white translucent.Wherein, circle position is by soda acid
It is formed with rear generated saline crystallization.
< impregnates saline solution step S4 >
It is that pH value is poured into the container is about 8.5 and comprising sodium bicarbonate in the immersion saline solution step S4
Saline solution about 400ml, makes the mixed liquid dipping in the saline solution, and stands until the mixture is formed as schemed
White collagen body shown in 4 left sides.
< cleans purification step S5 >
The collagen body is removed and placed in cleaning solution (water) from the container, gently drives the collagen egg
Lean type is moved repeatedly in clear water to be cleaned.Cleaning process lasts about greatly changes water after twenty minutes, and dries energy after being repeated 3 times
Obtain the collagen of a high-purity.By the collagen of obtained high-purity, with Western blot analyzing molecules amount, and will
Analysis result is recorded in Fig. 5.
" embodiment 2,3 "
Embodiment 2,3 is similar to Example 1, and different places are: in the immersion saline solution step S4, embodiment 2
Saline solution be pH value about 6.73 sodium chloride solution, the saline solution of embodiment 3 is that about 9.28 sodium dihydrogen phosphate of pH value is molten
Liquid.The obtained collagen body in the immersion saline solution step S4 of embodiment 2 is as shown in Figure 4, and embodiment 3 is in the leaching
Obtained collagen body is as shown in the right side Fig. 4 in bubble saline solution step S4.
" embodiment 4,5 "
Embodiment 4,5 is similar to Example 1, and different places are: in Yu Suoshu acid solution extraction step S2, embodiment 4
It is to be extracted using hydrochloric acid solution, embodiment 5 is extracted with acetum.In embodiment 4, walked in the drying process
The mixed liquor after adjustment pH value inactivates protease in rapid S3 is as shown in the left side Fig. 2, and in the drying process step
In S3 obtained mixture then as Fig. 3 a left side shown in.In embodiment 5, adjusting pH value in the drying process step S3 makes egg
The mixed liquor after white catabolic enzyme inactivation is as shown in Figure 2, and the obtained mixture in the drying process step S3
Then as shown in Figure 3.Western experiment equally is carried out for collagen obtained by embodiment 4,5, and by experimental result
Compared with embodiment 1 as shown in Figure 5.
Refering to fig. 1,4, it may see in the immersion saline solution step S4, whether using the described of sodium bicarbonate solution
Embodiment 1, the embodiment 2 using salt solution, or use the embodiment 3 of sodium dihydrogen phosphate, the mixture
After being immersed in the saline solution, the collagen body of white solid shape can be formed as illustrated in fig. 4.
It refering to fig. 1 to 3, can see from Fig. 2, in the drying process step S3, use the embodiment 5 of acetum
Collagen in the mixed liquor is soon precipitated after adjusting pH value, and the mixed liquor after the adjustment pH value of embodiment 1 is then
It is still in as clear as crystal, and then boundary is as clear as crystal between the two, and partially for the mixed liquor after the adjustment pH value of embodiment 4.From
It can then see in the drying process step S3, having using the obtained mixture of embodiment 5 of acetum in Fig. 3
Many larger-size holes, and the obtained mixture of embodiment 4 of hydrochloric acid solution is used, although with small size
Hole, but generally speaking continuity upper many good compared with embodiment 5.It can be seen that, the reality of citric acid solution is used from Fig. 3
The obtained mixture of example 1 is applied, in the optimal sheet of continuity/membranaceous.Due to the continuity of the mixture of embodiment 1,4
Preferably, therefore after impregnating saline solution it is formed by the collagen body, is cleaned in the cleaning purification step S5
When be less susceptible to scatter and melt, and can be reduced loss, and improve obtained collagen yield.
Refering to fig. 1,5, by Fig. 5 it is found that in the acid solution extraction step S2 whether use citric acid embodiment
1, using the embodiment of hydrochloric acid 4 or using the embodiment 5 of acetic acid, the comparable collagen of molecular weight distribution can be prepared.
In conclusion the effect of collagen processing method of the present invention is: providing a kind of can be effectively treated in pigskin
Collagen method, the collagen body can be made using pigskin, and purifying can be cleaned further purity is high is made
Collagen, used for related collagen industry, and promote the development of collagen industry.
Only as described above, only a specific embodiment of the invention cannot limit the model that the present invention is implemented with this
It encloses, it is all according to simple equivalent changes and modifications made by the claims in the present invention and description, all still belong to power of the present invention
Benefit requires in the range of covering.
Claims (12)
1. a kind of collagen processing method includes step A: providing clean pigskin as coarse fodder, it is characterised in that: the glue
Former albumen processing method also includes step B: the coarse fodder being mixed with protease, is placed in acid solution after extracting and filters
To obtain mixed liquor；Step C: adjust the mixed liquor pH value inactivate protease in the mixed liquor after, it is dry
The mixed liquor is to obtain mixture；And step D: the mixture is soaked in saline solution, to obtain by the mixing
The collagen body that object is formed.
2. collagen processing method according to claim 1, it is characterised in that: be clear to pigskin in Yu Suoshu step A
It washes, de-oiling, de-taste, sterilizing to obtain clean rough bark as coarse fodder.
3. collagen processing method according to claim 2, it is characterised in that: in Yu Suoshu step A, faced using super
Boundary's fluid is mixed with pigskin to slough the grease of pigskin and de-taste.
4. collagen processing method according to claim 3, it is characterised in that: the supercritical fluid is titanium dioxide
Carbon, and mixed under conditions of temperature is 30 DEG C~35 DEG C and pressure is 300bar~400bar with pigskin.
5. collagen processing method according to claim 1, it is characterised in that: the pH value of the acid solution be 2~
3。
6. collagen processing method according to claim 1, it is characterised in that: the protease is stomach cardia
Enzyme, thermophile protein enzyme, elastoser or any combination above-mentioned.
7. collagen processing method according to claim 1, it is characterised in that: the acid solution be hydrochloric acid solution,
Acetum, citric acid solution or any combination above-mentioned.
8. collagen processing method according to claim 1, it is characterised in that: the saline solution is that sodium chloride is molten
Liquid, sodium bicarbonate solution, sodium dihydrogen phosphate or any combination above-mentioned.
9. according to claim 1 to collagen processing method described in any claim in 8, it is characterised in that: Yu Suoshu
In step B, the acid solution is citric acid solution.
10. collagen processing method according to claim 9, it is characterised in that: the collagen processing method is also
Comprising in step E, the Yu Suoshu step E after the step D, being that the collagen body is driven to move repeatedly in cleaning solution
It is dynamic, to wash away the impurity in the collagen body, and obtain collagen.
11. collagen processing method according to claim 10, it is characterised in that: be that will adjust in Yu Suoshu step C
The mixed liquor after pH value is dried in container to obtain the mixture, is to pour into saline solution in Yu Suoshu step D
In the container, to obtain the collagen body formed by the mixture.
12. collagen processing method according to claim 11, it is characterised in that: be repeatedly clear in Yu Suoshu step E
The electrical conductivity until the collagen is washed less than 10 μ S/m.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TW106139008A TWI770078B (en) | 2017-11-10 | 2017-11-10 | Collagen treatment method |
| TW106139008 | 2017-11-10 |
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| CN109762060A true CN109762060A (en) | 2019-05-17 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113462736A (en) * | 2021-08-24 | 2021-10-01 | 华南师大(清远)科技创新研究院有限公司 | Preparation method for obtaining atelocollagen from pigskin and collagen |
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| TW201918493A (en) | 2019-05-16 |
| TWI770078B (en) | 2022-07-11 |
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