CN105983094A - Preparation method of sterile collagen liquid having bio-activity and preparation method of sterile collagen dressing having bio-activity - Google Patents
Preparation method of sterile collagen liquid having bio-activity and preparation method of sterile collagen dressing having bio-activity Download PDFInfo
- Publication number
- CN105983094A CN105983094A CN201510070015.XA CN201510070015A CN105983094A CN 105983094 A CN105983094 A CN 105983094A CN 201510070015 A CN201510070015 A CN 201510070015A CN 105983094 A CN105983094 A CN 105983094A
- Authority
- CN
- China
- Prior art keywords
- preparation
- bioactive
- collagen
- sterile collagen
- sterile
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Materials For Medical Uses (AREA)
Abstract
The invention discloses a preparation method of sterile collagen liquid having bio-activity and a preparation method of a sterile collagen dressing having bio-activity. The preparation method of the sterile collagen liquid comprises the following steps: degreasing, slicing and disinfecting animal tendons, crushing the animal tendons at low temperature, then conducting acid dissolution and enzymatic hydrolysis, conducting filtering and removing dregs, so that a collagen stock solution is obtained; and then, salting out and purifying the stock solution, so that the high-purity sterile collagen liquid which has the bio-activity is obtained; and the preparation method of the sterile collagen dressing is prepared by the following steps: filling the sterile collagen liquid in a non-woven fabric aluminum foil bag, sealing and freezing the bag, and then conducting low-temperature irradiation sterilization, so that the sterile collagen dressing having the bio-activity is obtained. The preparation method disclosed by the invention, by conducting irradiation under a low-temperature condition, can keep the activity of collagen and prevent denaturation or a cross-linking reaction in the collagen due to the irradiation; according to the preparation method disclosed by the invention, conventional dialysis purification is replaced by ultra-filtration purification, so that a purification time is shortened; and a conventional semiautomatic operation is replaced by an automatic system, so that people's hands are protected from getting contaminated, and the cleanliness, purity and bio-activity of the finished products (the sterile collagen liquid and the sterile collagen dressing) are guaranteed.
Description
Technical field
The present invention relates to a kind of there is bioactive sterile collagen liquid and the preparation method of sterile collagen sticking dressing.
Background technology
Collagen is twisted into spiral fibrous extracellular protein by three peptide chains, accounts for more than the 30% of whole body protein, has the physiologically active of uniqueness.Collagen protein is rich in aminoacid such as the glycine of needed by human, proline, hydroxyprolines, it it is most important ingredient in extracellular matrix, there is strong biological activity and biological function, the migration of cell can be participated in, break up and breed, bone tendon, cartilage and skin is made to have certain mechanical strength, and due to its faint antigenicity and good biocompatibility, of many uses at pharmaceutical sanitary fields such as burn, wound, cornea disease, beauty treatment, hard tissue repair, wound surface hemostasis.
Collagen is the protein that mammal in-vivo content is most, is distributed widely in the positions such as cornea, skin, skeleton, ligament, tendon, is the important feature albumen of composition connective tissue.Therefore extracting collagen from animal tissue is the important channel obtaining collagen.Can produce some side-product gelatin in the production process extracting collagen, gelatin forms by no longer having bioactive peptide chain after collagenous degeneration uncoiling.Why collagen has biological activity, depends primarily on its triple-helix structure, so gelatin is the most bioactive.
At present, the extracting method of collagen mainly has alkaline process, acid system, salt method and enzyme process.The advantage of alkalinity extraction is to be swift in response thoroughly, and its shortcoming is to cause collagen peptide bond hydrolysis, destroys the triple-helix structure of collagen, makes the aminoacid containing hydroxyl and sulfydryl destroy and produces racemization.Its D type aminoacid produced is poisonous, and have even has carcinogenic, teratogenesis and mutagenic harm, and therefore, alkalinity extraction collagen is not suitable for the extraction of bio-medical material collagen.Acidity extraction collagen mainly destroys the sat linkage between tropocollagen molecule and Schiff by low ion concns and sour environment
Key, so that collagen fiber are swelling, collagen is extracted in dissolving.Having an advantage in that the triple-helix structure keeping tropocollagen molecule to greatest extent, i.e. biological activity, it is low that its shortcoming is mainly productivity.It is that its technique is unstable that neutral sulfity process extracts the major defect of collagen so that the tropocollagen molecule structure of extraction is the most unstable.
The enzyme process of existing routine has the advantages such as hydrolysis is fast, environmentally friendly, and the collagen purity height extracted, physicochemical properties are stable, enzymolysis can excise the unspiralized region of tropocollagen molecule end peptide, reduce the antigenicity of collagen, but its shortcoming is to hydrolyze not thoroughly, and the biological activity of the collagen prepared is low.
The most medical collagen has strict requirements to microbial limit, and essentially all of product is desirable that and reaches sterilization grade, but provides and be difficulty with at present in technique.
Summary of the invention
In order to solve above-mentioned existing problems, the present invention, by research, uses final sterilization method to control the microbial limit of collagen and products thereof so that it is reach aseptic while the most do not change its biological activity, so that its application is safer, effectively.
It is an object of the invention to provide a kind of preparation method with bioactive sterile collagen liquid.
Another object of the present invention is to provide a kind of preparation method with bioactive sterile collagen sticking dressing.
The technical solution used in the present invention is:
A kind of preparation method with bioactive sterile collagen liquid, comprises the steps:
1) tendon of animal is carried out defat, cuts into slices, sterilize；
2) tendon after above-mentioned sterilization is pulverized under condition of ultralow temperature, obtain filamentous tendon fiber；
3) the tendon tissue after pulverizing carries out acid lyase solution, refilters the collagen stock solution that removes the gred to obtain；
4) collagen stock solution is saltoutd further, purification, i.e. can get and highly purified there is bioactive sterile collagen liquid.
Further, above-mentioned tendon is tendon Bovis seu Bubali tendon, pig tendon or rat-tail tendon.
Further, above-mentioned steps 2) described in pulverizing temperature be-150 DEG C～-60 DEG C.
Further, above-mentioned steps 3) described in sour lyase solution concrete operations by: the tendon fiber obtained is added in glacial acetic acid and pepsic mixed liquor, stirs 3 ~ 10 hours with the mixing speed of 10 ~ 50rpm.
Further, above-mentioned purification is to be purified by ultrafiltration the purification that system is carried out, and the condition of ultrafiltration system purification is: entrance point pressure is 10 ~ 40psi, and backflow end pressure is 0 ~ 10psi, carries out 6 ~ 12 times of ultrafiltration of constant volume.
Further, the above-mentioned whole preparation process with bioactive sterile collagen liquid is all carried out under ten thousand grades of environment.
A kind of preparation method with bioactive sterile collagen sticking dressing, comprises the following steps:
1) the bioactive sterile collagen liquid that has of preparation arbitrary in claim 1 ~ 6 is filled into containing in non-weaving cloth aluminium foil bag under 100,000 grades or above clean environment, seals；
2) aluminium foil bag previous step sealed carries out freezing；
3) aluminium foil bag after freezing is put into equipped with in dry ice or the bubble chamber of ultralow temperature ice chest or couveuse and seal, carry out low temperature irradiation sterilizing, after defrosting, i.e. can obtain that there is bioactive sterile collagen sticking dressing.
Further, above-mentioned steps 2) described in freezing temperature be-80 DEG C～-10 DEG C, cooling time is 18～72h.
Further, above-mentioned steps 3) described in the irradiation dose of low temperature irradiation sterilizing be 15 ~ 40kGy, temperature is-75 DEG C～0 DEG C.
Any of the above-described preparation there is the application in skin injury repairs product, wound repair product, medical cosmetology product or cosmetics of the bioactive sterile collagen sticking dressing.
The invention has the beneficial effects as follows:
1) present invention carries out irradiation sterilization to collagen solution or collagen plaster dressing under cryogenic, and product can be made to reach aseptic (sterility assurance level SAL=10-6While), keep the activity of collagen, it is to avoid collagen deactivation or crosslink the function reduction that reaction causes and even inactivate during irradiation.
2) present invention is purified by ultrafiltration and replaces traditional dialysis purification, and effect is to shorten the time of purification, additionally use automated system to replace traditional semi-automatic operation, it is to avoid people's hand contamination, it is ensured that the cleanliness factor of product, purity, biological activity.
3) animal tissue is pulverized under condition of ultralow temperature by the present invention, disrupting tissue is powdered or filament shape, greatly add the contact area of reaction during making to be organized in acid lyase solution, make the time of reaction shorten and improve the biological activity that productivity, beneficially collagen remain intact.
Accompanying drawing explanation
Fig. 1 is collagen solution and collagen plaster dressing people's SDS-PAGE electrophoresis detection of preparing of embodiment of embodiment 1 preparation, and M is sigma standard substance, and 1 be the collagen solution of embodiment 1 preparation, and 2 is collagen plaster dressing prepared by embodiment 2.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further illustrated, but is not limited thereto.
Embodiment
1
There is the preparation method of bioactive sterile collagen liquid
1) fresh healthy tendon Bovis seu Bubali tendon, excision oils and fats removes fascia, cleans and go the removal of impurity, cuts into slices, sterilize, save backup after freezing
2) take the tendon Bovis seu Bubali tendon after above-mentioned sterilization to pulverize under the conditions of-80 DEG C～-60 DEG C, obtain filamentous tendon fiber；
3) adding in tendon fiber in the glacial acetic acid of 0.5mol/L and the pepsic mixed liquor of 400mg/L of 100 times of volumes, continuously stirred 3 hours, ambient temperature range was 25～28 DEG C, mixing speed 50rpm；
Above-mentioned mixed liquor filter cleaner is obtained collagen stock solution；
4) sodium chloride solution that above-mentioned collagen stock solution adds isopyknic 1.4mol/L is saltoutd, and takes precipitation, and the glacial acetic acid that precipitation rejoins 0.5mol/L dissolves,
Being purified by ultrafiltration apparatus, the condition of ultrafiltration system is: mouth end pressure is 40psi, and backflow end is 10psi, carries out 6 times of ultrafiltration of constant volume and i.e. can get and highly purified have bioactive sterile collagen liquid.
Embodiment
2
: there is the preparation method of bioactive sterile collagen sticking dressing
1) use semi-automatic filling apparatus to carry out fill under 100,000 grades or above clean environment the bioactive sterile collagen liquid that has of preparation in embodiment 1, be filled into equipped with in non-weaving cloth aluminium foil bag, seal；The most each aluminium foil bag loads the collagen solution of 30ml, and being shaped as of non-weaving cloth is square, a size of 20cm × 10cm；
6) aluminium foil bag good for above-mentioned sealing is carried out below-18 DEG C freezing 24 hours；
7) the above-mentioned aluminium foil bag freezed is loaded in couveuse, couveuse loads 4 ultralow temperature ice chests, makes the temperature of couveuse maintain less than 0 DEG C state, seal thermal-insulation box, deliver to Radiation Center and carry out the irradiation of 40kgy, after thaw at RT, i.e. can obtain that there is bioactive collagen plaster dressing.
Above-mentioned have bioactive sterile collagen sticking dressing and can be applicable to the reparation of wound surface, such as burn wound, scald wound, laser beautifying etc..
Embodiment
3
There is the preparation method of bioactive sterile collagen liquid
1) fresh health pig tendon, excision oils and fats removes fascia, cleans and go the removal of impurity, cuts into slices, sterilize, save backup after freezing
2) take the tendon after above-mentioned sterilization to pulverize under the conditions of-150 DEG C～-100 DEG C, obtain filamentous tendon fiber；
3) adding in tendon fiber in the glacial acetic acid of 0.4mol/L and the pepsic mixed liquor of 300mg/L of 150 times of volumes, continuously stirred 10 hours, ambient temperature range was 25～27 DEG C, mixing speed 10rpm；
Above-mentioned mixed liquor filter cleaner is obtained collagen stock solution；
4) sodium chloride solution that above-mentioned collagen stock solution adds isopyknic 1.4mol/L is saltoutd, take precipitation, the glacial acetic acid that precipitation rejoins 0.4mol/L dissolves, it is purified by ultrafiltration apparatus, the condition of ultrafiltration system is: entrance point pressure is 30psi, backflow end is 5psi, carries out 12 times of ultrafiltration of constant volume and i.e. can get and highly purified have bioactive sterile collagen liquid.
Embodiment
4
: there is the preparation method of bioactive sterile collagen sticking dressing
1) the bioactive sterile collagen liquid that has of preparation in embodiment 3 is carried out fill under 100,000 grades or above clean environment, load in the aluminium foil bag containing non-weaving cloth, sealing；The most each aluminium foil bag loads the collagen solution of 25ml, and non-weaving cloth is circular, a diameter of 21.5cm；
2) by good for above-mentioned sealing-80 DEG C of freezings of aluminium foil bag more than 18 hours；
3) the above-mentioned aluminium foil bag freezed is loaded in couveuse, couveuse loads the dry ice of 4kg, makes the temperature of couveuse maintain less than 0 DEG C low temperature shape, seal thermal-insulation box, deliver to Radiation Center and carry out the irradiation of 15kgy, after thaw at RT, i.e. can obtain that there is bioactive sterile collagen sticking dressing.
Above-mentioned have bioactive sterile collagen sticking dressing and can be applicable to the reparation of wound surface, such as burn wound, scald wound, laser beautifying etc..
Embodiment
5
: there is the preparation method of bioactive sterile collagen liquid
1) fresh healthy rat-tail tendon, excision oils and fats removes fascia, cleans and go the removal of impurity, and cutting after freezing is sterilized, saved backup
2) take the tendon after above-mentioned sterilization to pulverize under the conditions of-110 DEG C～-90 DEG C, obtain filamentous tendon fiber；
3) adding in tendon fiber in the glacial acetic acid of 0.1mol/L and the pepsic mixed liquor of 400mg/L of 200 times of volumes, continuously stirred 6 hours, ambient temperature range was 25～28 DEG C, mixing speed 15rpm；
Above-mentioned mixed liquor filter cleaner is obtained collagen stock solution；
4) sodium chloride solution that above-mentioned collagen stock solution adds isopyknic 1.4mol/L is saltoutd, take precipitation, the glacial acetic acid that precipitation rejoins 0.2mol/L dissolves, it is purified by ultrafiltration apparatus, the condition of ultrafiltration system is: mouth end pressure is 30psi, backflow end is 0psi, carries out 8 times of ultrafiltration of constant volume and i.e. can get and highly purified have bioactive sterile collagen liquid.
Embodiment
6
There is the preparation method of bioactive sterile collagen sticking dressing
1) the bioactive sterile collagen liquid that has of preparation in embodiment 5 is carried out fill under 100,000 grades or above clean environment, load in the aluminium foil bag containing non-weaving cloth, sealing；The most each aluminium foil bag loads the collagen solution of 35ml, and non-weaving cloth is oval, a diameter of 9.5cm × 6cm；
2) by aluminium foil bag good for above-mentioned sealing freezing 20 hours below-10 DEG C；
7) the above-mentioned aluminium foil bag freezed is loaded in couveuse, couveuse loads 6 ultralow temperature ice chests, the temperature making couveuse maintains less than-75 DEG C low temperature states, seal thermal-insulation box, deliver to Radiation Center and carry out the irradiation of 27kgy, after thaw at RT, i.e. can obtain that there is bioactive sterile collagen sticking dressing.
Above-mentioned have bioactive sterile collagen sticking dressing and can be applicable to the reparation of wound surface, such as burn wound, scald wound, laser beautifying etc..
SDS-PAGE
Electrophoresis detection
Have bioactive sterile collagen liquid separately below to embodiment 1 preparation, the bioactive sterile collagen sticking dressing that has of embodiment 2 preparation carries out SDS-PAGE electrophoresis detection, if testing result is looked into shown in Fig. 1.From figure 1 it appears that collagen protein has good biological activity and purity in the collagen solution prepared of the present invention and collagen plaster dressing.
Cytotoxicity detects
The bioactive sterile collagen sticking dressing that has preparing above-described embodiment carries out biological property detection, result shows that the score of its cytotoxicity is not more than 1, without sensitivity response, without Intradermal irritant reaction or pole minimal irritation, without sub-chronic systemic toxicity, and hereditary-less toxicity.
Microorganism and chemical property detection
The bioactive sterile collagen sticking dressing that has preparing above-described embodiment carries out microorganism detection, and result shows aseptic.
The bioactive sterile collagen sticking dressing that has preparing above-described embodiment carries out chemical property detection, and result shows, has typical I-type collagen electrophoresis pattern, and its total metals is not more than 10 μ g/g.
Wound repair effect detects
Utilize prepared by above-described embodiment to have bioactive sterile collagen sticking dressing treatment shallow II degree burn wound random, comparison, multicenter clinical trial results: this test sample amount is 160 examples, 6 institution of clinical trials are selected to test altogether, test apparatus is collagen plaster dressing, comparison apparatus is ventolin dressing, experimental result display collagen plaster dressing can comparatively fast shorten the healing time of shallow II degree burn wound, improve local symptom sign, wound healing, improve the comprehensive healing rate of wound surface, thus alleviate patient's misery during wound repair, clinic popularization and application.
Easy to understand for those skilled in the art; the foregoing is only the preferred embodiment of patent of the present invention; not in order to limit the present invention, any amendment, equivalent and the improvement etc. made within all the spirit and principles in the present invention, within all falling within the protection domain of application claims.
Claims (10)
1. a preparation method with bioactive sterile collagen liquid, it is characterised in that: comprise the steps:
1) tendon of animal is carried out defat, cuts into slices, sterilize；
2) tendon after above-mentioned sterilization is pulverized under condition of ultralow temperature, obtain filamentous tendon fiber；
3) the tendon tissue after pulverizing carries out acid lyase solution, refilters the collagen stock solution that removes the gred to obtain；
4) collagen stock solution is saltoutd further, purification, i.e. can get and highly purified there is bioactive sterile collagen liquid.
A kind of preparation method with bioactive sterile collagen liquid the most according to claim 1, it is characterised in that: described tendon is tendon Bovis seu Bubali tendon, pig tendon or rat-tail tendon.
A kind of preparation method with bioactive sterile collagen liquid the most according to claim 1, it is characterised in that: step 2) described in pulverizing temperature be-150 DEG C～-60 DEG C.
A kind of preparation method with bioactive sterile collagen liquid the most according to claim 1, it is characterized in that: the concrete operations of the sour lyase solution described in step 3) by: the tendon fiber that obtained is added in glacial acetic acid and pepsic mixed liquor, stirs 3 ~ 10 hours with the mixing speed of 10 ~ 50rpm.
A kind of preparation method with bioactive sterile collagen liquid the most according to claim 1, it is characterized in that: described purification is to be purified by ultrafiltration the purification that system is carried out, the condition of ultrafiltration system purification is: entrance point pressure is 10 ~ 40psi, backflow end pressure is 0 ~ 10psi, carries out 6 ~ 12 times of ultrafiltration of constant volume.
A kind of preparation method with bioactive sterile collagen liquid the most according to claim 1, it is characterised in that: the above-mentioned whole preparation process with bioactive sterile collagen liquid is all carried out under 100,000 grades or environment above.
7. a preparation method with bioactive sterile collagen sticking dressing, it is characterised in that: comprise the following steps:
1) the bioactive sterile collagen liquid that has of preparation arbitrary in claim 1 ~ 6 is filled into containing in non-weaving cloth aluminium foil bag under 100,000 grades or above clean environment, seals；
2) aluminium foil bag previous step sealed carries out freezing；
3) aluminium foil bag after freezing is put into equipped with in dry ice or the bubble chamber of ultralow temperature ice chest or couveuse and seal, carry out low temperature irradiation sterilizing, after defrosting, i.e. can obtain that there is bioactive sterile collagen sticking dressing.
A kind of preparation method with bioactive sterile collagen sticking dressing the most according to claim 7, it is characterised in that: step 2) described in freezing temperature be-80 DEG C～-10 DEG C, cooling time is 18～72h.
A kind of preparation method with bioactive sterile collagen sticking dressing the most according to claim 7, it is characterised in that: the irradiation dose of low temperature irradiation sterilizing described in step 3) is 15 ~ 40kGy, and temperature is-75 DEG C～0 DEG C.
10. in claim 7 ~ 9, the bioactive sterile collagen sticking dressing that has of arbitrary preparation repairs the application in product, wound repair product, medical cosmetology product or cosmetics preparing skin injury.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510070015.XA CN105983094A (en) | 2015-02-09 | 2015-02-09 | Preparation method of sterile collagen liquid having bio-activity and preparation method of sterile collagen dressing having bio-activity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510070015.XA CN105983094A (en) | 2015-02-09 | 2015-02-09 | Preparation method of sterile collagen liquid having bio-activity and preparation method of sterile collagen dressing having bio-activity |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105983094A true CN105983094A (en) | 2016-10-05 |
Family
ID=57041853
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510070015.XA Pending CN105983094A (en) | 2015-02-09 | 2015-02-09 | Preparation method of sterile collagen liquid having bio-activity and preparation method of sterile collagen dressing having bio-activity |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105983094A (en) |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106578332A (en) * | 2016-11-24 | 2017-04-26 | 湖州珍露生物制品有限公司 | Preparation technology of elastin powder |
| CN107137750A (en) * | 2017-04-14 | 2017-09-08 | 绍兴金石生物工程有限公司 | A kind of preparation method of bioactivity application |
| CN107441027A (en) * | 2017-09-13 | 2017-12-08 | 黑龙江聚拢华玺智能科技有限公司 | A kind of moisturizing cleawhite pack |
| CN107684532A (en) * | 2017-09-28 | 2018-02-13 | 武汉医佳宝生物材料有限公司 | A kind of active collagen facial mask and preparation method |
| CN109069697A (en) * | 2016-12-16 | 2018-12-21 | 厦门大开生物科技有限公司 | A kind of irradiation sterilization method and its sterilizing cornea of cornea |
| CN109106610A (en) * | 2018-08-13 | 2019-01-01 | 浙江珂瑞康生物医疗科技有限公司 | A kind of sterilization process of no antiseptic activity collagen mask |
| WO2019169646A1 (en) * | 2018-03-07 | 2019-09-12 | 广州创尔生物技术股份有限公司 | Quality control method for biologically active collagen |
| WO2019174652A3 (en) * | 2018-03-16 | 2019-10-31 | 广东众尔健生物科技有限公司 | Application of rat tail collagen in field of cosmetics |
| CN114306719A (en) * | 2021-12-30 | 2022-04-12 | 江苏知原药业股份有限公司 | Collagen plaster dressing and preparation method thereof |
| CN114469754A (en) * | 2022-01-25 | 2022-05-13 | 杭州协合医疗用品有限公司 | Aseptic active collagen mask paste and sterilization method thereof |
| CN115137868A (en) * | 2021-03-30 | 2022-10-04 | 西安德诺海思医疗科技有限公司 | Recombinant collagen dressing and preparation method thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1508154A (en) * | 2002-12-18 | 2004-06-30 | 和康生物科技股份有限公司 | Method for preparing injection collagen, and product and use thereof |
| CN101810855A (en) * | 2010-05-20 | 2010-08-25 | 佟刚 | II-type collagen joint cartilage fluid and preparation method thereof |
| CN101874903A (en) * | 2009-11-20 | 2010-11-03 | 胡庆柳 | Method for preparing collagen artificial skin |
| CN102131403A (en) * | 2009-07-27 | 2011-07-20 | 成功大学 | Preparation of high purity collagen |
| CN103143052A (en) * | 2013-03-13 | 2013-06-12 | 潘华倩 | Medical collagen dressing as well as preparation method and use thereof |
| CN103566409A (en) * | 2012-07-27 | 2014-02-12 | 高广田 | Preparation method and application of medical collagen retaining biological activity |
| CN103966294A (en) * | 2013-02-02 | 2014-08-06 | 深圳兰度生物材料有限公司 | Extraction method for bioactive collagen |
-
2015
- 2015-02-09 CN CN201510070015.XA patent/CN105983094A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1508154A (en) * | 2002-12-18 | 2004-06-30 | 和康生物科技股份有限公司 | Method for preparing injection collagen, and product and use thereof |
| CN102131403A (en) * | 2009-07-27 | 2011-07-20 | 成功大学 | Preparation of high purity collagen |
| CN101874903A (en) * | 2009-11-20 | 2010-11-03 | 胡庆柳 | Method for preparing collagen artificial skin |
| CN101810855A (en) * | 2010-05-20 | 2010-08-25 | 佟刚 | II-type collagen joint cartilage fluid and preparation method thereof |
| CN103566409A (en) * | 2012-07-27 | 2014-02-12 | 高广田 | Preparation method and application of medical collagen retaining biological activity |
| CN103966294A (en) * | 2013-02-02 | 2014-08-06 | 深圳兰度生物材料有限公司 | Extraction method for bioactive collagen |
| CN103143052A (en) * | 2013-03-13 | 2013-06-12 | 潘华倩 | Medical collagen dressing as well as preparation method and use thereof |
Non-Patent Citations (1)
| Title |
|---|
| 孙继玮等: "胶原贴敷料联合异维A酸红霉素凝胶治疗寻常痤疮疗效观察", 《中国麻风皮肤病杂志》 * |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106578332A (en) * | 2016-11-24 | 2017-04-26 | 湖州珍露生物制品有限公司 | Preparation technology of elastin powder |
| CN109069697A (en) * | 2016-12-16 | 2018-12-21 | 厦门大开生物科技有限公司 | A kind of irradiation sterilization method and its sterilizing cornea of cornea |
| CN107137750A (en) * | 2017-04-14 | 2017-09-08 | 绍兴金石生物工程有限公司 | A kind of preparation method of bioactivity application |
| CN107137750B (en) * | 2017-04-14 | 2020-04-10 | 绍兴金石生物工程有限公司 | Method for manufacturing bioactive plaster |
| CN107441027A (en) * | 2017-09-13 | 2017-12-08 | 黑龙江聚拢华玺智能科技有限公司 | A kind of moisturizing cleawhite pack |
| CN107684532A (en) * | 2017-09-28 | 2018-02-13 | 武汉医佳宝生物材料有限公司 | A kind of active collagen facial mask and preparation method |
| WO2019169646A1 (en) * | 2018-03-07 | 2019-09-12 | 广州创尔生物技术股份有限公司 | Quality control method for biologically active collagen |
| WO2019174652A3 (en) * | 2018-03-16 | 2019-10-31 | 广东众尔健生物科技有限公司 | Application of rat tail collagen in field of cosmetics |
| CN109106610A (en) * | 2018-08-13 | 2019-01-01 | 浙江珂瑞康生物医疗科技有限公司 | A kind of sterilization process of no antiseptic activity collagen mask |
| CN109106610B (en) * | 2018-08-13 | 2021-09-03 | 浙江珂瑞康生物医疗科技有限公司 | Sterilization process of collagen mask without preservative activity |
| CN115137868A (en) * | 2021-03-30 | 2022-10-04 | 西安德诺海思医疗科技有限公司 | Recombinant collagen dressing and preparation method thereof |
| CN115137868B (en) * | 2021-03-30 | 2023-10-20 | 西安德诺海思医疗科技有限公司 | Recombinant collagen dressing and preparation method thereof |
| CN114306719A (en) * | 2021-12-30 | 2022-04-12 | 江苏知原药业股份有限公司 | Collagen plaster dressing and preparation method thereof |
| CN114469754A (en) * | 2022-01-25 | 2022-05-13 | 杭州协合医疗用品有限公司 | Aseptic active collagen mask paste and sterilization method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105983094A (en) | Preparation method of sterile collagen liquid having bio-activity and preparation method of sterile collagen dressing having bio-activity | |
| CN105985429A (en) | Aseptic collagen liquid with biological activity and preparation method thereof | |
| CN103432627B (en) | Method for preparing animal acellular tissue matrix material and tissue matrix material prepared by same | |
| JP6889118B2 (en) | Manufacture of high-purity collagen particles and their use | |
| CN103251987B (en) | Acellular biological patch, preparation method and apparatus thereof | |
| US20100291532A1 (en) | Process for sterilizing acellular soft tissue with irradiation | |
| KR101410533B1 (en) | A method for treating material derived from biological tissue | |
| JP6899455B2 (en) | How to manufacture and use collagen for cartilage tissue repair | |
| CN104740685A (en) | Nerve repairing film and preparation method thereof | |
| JP7378486B2 (en) | Medical adhesives, their preparation methods, and their uses | |
| WO2008154621A2 (en) | Process for sterilizing acellular soft tissue under pressure | |
| CN104825493A (en) | Biological sheep membrane for ocular surface treatment and preparation method thereof | |
| CN104721877B (en) | A kind of sterile collagen sticking dressing and preparation method thereof | |
| CN105524965A (en) | Extraction method of bovine tendon collagen | |
| CN103418001B (en) | The method for disinfection and sterilization of a kind of animal tissue material and corresponding animal tissue soaking solution | |
| CN109589446A (en) | A kind of absorbable biological hemostatic material and preparation method thereof | |
| CN104474573A (en) | Biological mask and preparation method thereof | |
| KR101139434B1 (en) | Manufacturing method for dressing material using porcine skin | |
| CN108079363A (en) | A kind of kit and its application that cell processing is taken off for animal tissue | |
| CN110638684A (en) | Injectable collagen composition and preparation method thereof | |
| RU2691983C1 (en) | Method for purification, modification and sterilization of bone tissue and skin matrix derivatives using supercritical fluid | |
| CN110075356A (en) | A kind of plasticity packing material and preparation method thereof for surgical intervention bone defect | |
| WO2022093877A1 (en) | Preparation and use of tissue matrix derived powder | |
| CN114146224A (en) | Preparation method of acellular dermal matrix particles | |
| CN102178936A (en) | Methods for preparing collagen oleostock and moistening eyedrops |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20161005 |