CN108478745A

CN108478745A - One seed ginseng Chinese herbaceous peony nourishing qi and blood particle Chinese medicine composition and preparation method thereof

Info

Publication number: CN108478745A
Application number: CN201810570882.3A
Authority: CN
Inventors: 郝彬
Original assignee: SHAANXI HAO QI JUN PHARMACEUTICAL CO Ltd
Current assignee: SHAANXI HAO QI JUN PHARMACEUTICAL CO Ltd
Priority date: 2018-06-05
Filing date: 2018-06-05
Publication date: 2018-09-04

Abstract

The invention discloses seed ginseng Chinese herbaceous peony nourishing qi and blood particle Chinese medicine compositions and preparation method thereof, the Chinese medicine composition is that totally ten two taste bulk pharmaceutical chemicals are prepared by red ginseng, Radix Paeoniae Alba, Radix Astragali, prepared rehmannia root, Radix Angelicae Sinensis, Herba Epimedii, Morinda officinalis, teasel root, Semen Cuscutae, hairyvein agrimony, rhizoma atractylodis macrocephalae, fructus amomi, successively through water extraction, clear cream drying, crushing, addition dextrin and sucrose mixing, packing, obtained granule, better efficacy of the present invention, compatibility rationally, collaboration play a role, clear efficacy, safety it is without any side effects, it takes for a long time safe, not will produce dependence.The present invention has effects that nourishing generate fluid, strong marrow green blood, strengthening yang and invigorating kidney, good efficacy to suffering from a deficiency of the kidney.Cytopenia, card is caused to see qi-blood deficiency, the insufficiency of the kidney yang for treating alpastic anemia, myelodysplastic syndrome, thrombopenia and chemicotherapy.

Description

One seed ginseng Chinese herbaceous peony nourishing qi and blood particle Chinese medicine composition and preparation method thereof

Technical field

The present invention relates to seed ginseng Chinese herbaceous peony nourishing qi and blood particle Chinese medicine compositions and preparation method thereof, belong to pharmaceutical technology neck Domain.

Background technology

Alpastic anemia (AA) is quantity reduction or the work(for leading to candidate stem cell by many reasons referred to as " hindering again " Can be abnormal, so as to cause a kind of syndrome of PB red blood cells, leucocyte and decrease of platelet.Its for hematological system common disease, Frequently-occurring disease, the disease that 12.6% and a kind of course of disease that account for disease in the blood system are long, treatment is more difficult, clinic with anaemia, go out Blood and infection are main performance.Data are shown according to investigations, and worldwide frequency is ten thousand populations of 0.2-0.5/10, U.S. and other Industrial country is 1.2/10 ten thousand populations.The annual morbidity that China hinders again is 0.74/10 ten thousand populations, and wherein chronic aplastic anemia incidence is 0.6/10 ten thousand populations, acute aplastic anemia are 0.14/10 ten thousand populations；Wherein chronic aplastic anemia (CAA) onset is slow, incidence of occult, disease Journey is long, touching not to be cured, if without active and effective treatment, some easily switchs to acute aplastic anemia (AAA), and the state of an illness is critical, and prognosis is dangerous.

Currently, western medicine alpastic anemia, mainly uses male sex hormone：Such as third climbs, stanozolol, pacifies hero, exempts from Epidemic disease inhibitor：Such as antithymocyte globulin, antilymphocyte globulin (ALG) encircle rhzomorph A, hemopoieticgrowth factor：As EPO, GM-CSF, G-CSF etc. and some other therapies, such as umbilical serum, fetal-liver infusion application so that patient CAA is prolonged Long survival rate, and then improve curative effect.But often the generation of side effect is gone hand in hand with therapeutic effect, and common side reaction has The damage of Liver and kidney function, digestive tract reaction, hirsutism, nerve endings cacesthesia etc., or even can also result in Liver and kidney function and decline It exhausts.There are many Chinese medicine composition of the Chinese medicine in terms for the treatment of alpastic anemia, but curative effect is less desirable mostly, the prior art Disclosed in Chinese patent publication：1, CN106214828A discloses a kind of Chinese medicine of passage, nourishing blood and preparation method thereof, bulk pharmaceutical chemicals By red ginseng, Radix Angelicae Sinensis, radix rehmanniae preparata, Radix Salviae Miltiorrhizae, Radix Astragali, psoralea corylifolia, Caulis Spatholobi, giant knotweed, Herba Cistanches, Morinda officinalis, Semen Cuscutae, longspur epimedium, meat Totally 13 taste Chinese medicines form in osmanthus, and decoction, pill, capsule, tablet or oral solution are processed by techniques such as extractions；For controlling Treat decrease of platelet, alpastic anemia, myelodysplastic syndrome, after malignant tumor chemotherapy caused by anaemia and long Disease causes deficient illness.2, CN103933203A disclose it is a kind of for treat alpastic anemia compound Hematogenesis capsule and its Preparation method, bulk pharmaceutical chemicals are by red ginseng, hippocampus, Radix Astragali, Semen Cuscutae, Radix Angelicae Sinensis, radix rehmanniae preparata, Herba Epimedii, the fruit of glossy privet, Rhizoma Chuanxiong, Caulis Spatholobi, mountain Totally 14 taste Chinese medicines form for short, bristly hair or beard, donkey-hide gelatin, garden burnet, Radix Glycyrrhizae, and capsule is processed into through crushing and extracting；For treating aregeneratory Property anaemia and malnutritional anemia.3, CN1539459A discloses a kind of reconstruction green blood Chinese medicine preparation that treating anaemia and preparation Method, bulk pharmaceutical chemicals by wine Semen Cuscutae, go Lu Hongcan, Caulis Spatholobi, donkey-hide gelatin, Radix Angelicae Sinensis, the fruit of glossy privet, Radix Astragali, motherwort, prepared rehmannia root, Radix Paeoniae Alba, prepared fleece flower root, Herba Epimedii, wine RHIZOMA POLYGONATI PREPARATA, unhairing pilose antler, Radix Codonopsis, Radix Ophiopogonis, hairyvein agrimony, rhizoma atractylodis macrocephalae, salt psoralea corylifolia, Chinese holly Totally 21 taste Chinese medicines form for matrimony vine, eclipta, and the agent such as tablet, capsule, granule, pill are processed into through crushing and extracting Type；For treating a variety of anaemia diseases such as aplastic anemia, hypoferric anemia, Neuroleptic Leukocytopenia.Inventor does the prior art Numerous studies find that the effect of 3 kinds are treated alpastic anemia disclosed in the above patent gazette is undesirable, and have some pairs Effect.Therefore, exploitation one kind is using Chinese herbal medicine as raw material, better efficacy, and compatibility is reasonable, collaboration plays a role, clear efficacy, safety Treatment alpastic anemia without any side effects, and to myelodysplastic syndrome, thrombopenia and putting It treats and cytopenia is caused also to have the Chinese medicine composition of good therapeutic effect, will certainly have broad application prospects and the market space.

Invention content

The object of the present invention is to provide the systems of a seed ginseng Chinese herbaceous peony nourishing qi and blood particle Chinese medicine composition and the Chinese medicine composition Preparation Method.

The present invention join Chinese herbaceous peony nourishing qi and blood particle the effect of be：It is strong with nourishing generate fluid, strong marrow green blood, strengthening yang and invigorating kidney, tonifying spleen The effect of stomach.Cause blood thin for treating alpastic anemia, myelodysplastic syndrome, thrombopenia and chemicotherapy Born of the same parents reduce disease.Card is shown in qi-blood deficiency, the insufficiency of the kidney yang.

According to seed ginseng Chinese herbaceous peony nourishing qi and blood particle Chinese medicine composition provided by the present invention and preparation method thereof, feature exists It is made of following raw material medicaments in it：

Red ginseng, Radix Paeoniae Alba, Radix Astragali, prepared rehmannia root, Radix Angelicae Sinensis, Herba Epimedii, Morinda officinalis, teasel root, Semen Cuscutae, hairyvein agrimony, rhizoma atractylodis macrocephalae, sand Benevolence.

According to seed ginseng Chinese herbaceous peony nourishing qi and blood particle Chinese medicine composition provided by the present invention and preparation method thereof, feature exists It is made of the bulk pharmaceutical chemicals of following weight proportioning in it：

According to seed ginseng Chinese herbaceous peony nourishing qi and blood particle Chinese medicine composition provided by the present invention and preparation method thereof, feature exists It is made in the bulk pharmaceutical chemicals that it is matched by following preferred weights：

A seed ginseng Chinese herbaceous peony nourishing qi and blood particle Chinese medicine composition and preparation method thereof according to the present invention, preparation method Include the following steps：

Above 12 taste adds water to cook secondary, 1.5 hours first times, adds 8 times of amounts of water, second 1 hour, adds 6 times of water Amount, collecting decoction, filtration, filtrate measure the clear cream that relative density is 1.20-1.25 when being condensed into 60 DEG C, 70 DEG C are dried under reduced pressure, It is ground into fine powder；Take extract powder, dextrin, cane sugar powder by 1:0.5:Particle is made with 80% ethyl alcohol in 1 ratio mixing, dry, whole 1000g is made in grain, dispenses to get granule.

12 kinds of Chinese medicine effects of the present invention and pharmacological action are as follows：

Red ginseng：This product is drying of the cultivation product of Araliaceae ginseng Panax ginseng C.A.Mey. after steaming Root and rhizome.Property sweet, slight bitter, temperature.Returns spleen, lung, the heart, kidney channel.Function is reinforced vital energy, veins takes off admittedly, nourishing qi to stop.For physically weak It is intended to take off, cold weak pulse of limb is Qi dysfunction in blood control, metrostaxis.First recorded in《Sheng Nong's herbal classic》、《Haigoushen》、《Mingyi Bielu》In also have It records.Modern pharmacology research shows to mainly contain a variety of triterpene saponin componds, polysaccharide and polypeptide compounds.Have anti- Anaphylactic shock and cardiac effect can improve immunity, improve anaemia effect, can also make the ratio of plasma albumin and globulin Rise.

Radix Paeoniae Alba：This product is the dry root of ranunculaceae plant Chinese herbaceous peony Paeonia lactiflora Pall., sets in boiling water and boils It boils, dries again after removing crust or peeling afterwards.Property bitter, acid be slightly cold.Return liver, the spleen channel.Function nourishing blood for regulating menstruation, astringing YIN to stop sweating, soft liver Analgesic, suppressing liver-YANG.For blood deficiency chlorosis, irregular menstruation, spontaneous perspiration, night sweat, hypochondriac pain, abdominal pain, four limbs contraction pain, dizziness of having a headache.《Medicine Property opinion》、《Mingyi Bielu》With《Japan hanako materia medica》It is on the books.Modern pharmacology research shows to mainly contain containing a variety of monoterpenes And its glycosides compound.There is apparent analgesic activity, has inhibition and spasmolysis to smooth muscle；There is bidirectional modulation work to immune function With；Blood vessel can be expanded, blood flow is increased；Antibacterial action is strong, has a broad antifungal spectrum.

Radix Astragali：This product is legume astragalus mongolicus Astragalus membranaceus (Fisch.) Bge.var. The dry root of mongholicus (Bge.) Hsiao or Astragalus membranacus Astragalus membranaceus (Fisch.) Bge.. Sweet, the tepor of property.Returns spleen, lung channel.Function tonifying Qi and lifting yang, strengthening exterior and reducing sweat, inducing diuresis for removing edema, shengjin nourishing, move stagnation and palsy, eliminating toxic row Purulence, expelling pus and promoting granulation.For treatment deficiency of vital energy weak, anorexia and loose stool, the sinking of qi of middle-jiao, rush down prolapse of the anus for a long time, uterine bleeding of having blood in stool, exterior deficiency spontaneous perspiration, deficiency of vital energy water It is swollen, Heat Diabetes, blood deficiency chlorosis, hemiplegia, the logical numbness of numbness, ulcer is difficult to burst, burst for a long time and does not holds back.《Sheng Nong's herbal classic》、《Name doctor is other Record》、《Compendium of Materia Medica》Deng on the books.Modern pharmacology research shows to mainly contain a variety of saponin(es, flavones and polysaccharide chemical combination Object.Body immunity can be improved, antiviral, anti-aging has protection and facilitation to hematopoiesis function, can prevent and treat painstaking effort Pipe disease.

Prepared rehmannia root：The dry root that this product is scrophulariaceae rehmannia glutinosa plant Rehmannia glutinosa Libosch. is through processing Processed goods.Sweet, the tepor of property.Return liver and kidney channel.Function is enriched blood enriching yin, beneficial to spirit and marrow.For blood deficiency chlorosis, palpitation and severe palpitation, menstruation It is uncomfortable, metrostaxis, the deficiency of liver-yin and kidney-yin, soreness and weakness of waist and knees, osteopyrexia and fever, night sweat seminal emission, Heat Diabetes, dizziness, tinnitus, beard and hair morning In vain.First recorded in《Sheng Nong's herbal classic》, modern pharmacology research shows to mainly contain iridoid glycosides, flavonoids and polysaccharide Compound.With a variety of effects such as resisting cardiovascular disease, neuroprotection, anti-diabetic and complication, anti-osteoporosis.

Radix Angelicae Sinensis：This product is the dry root of umbelliferae angelica Angelica sinensis (Oliv.) Diels.Property is sweet, It is acrid in flavour and warm in nature.Return liver, the heart, the spleen channel.Function replenishing and activating blood, menstruction regulating and pain relieving relax bowel.For blood deficiency chlorosis, dizziness palpitaition, menstruation It is uncomfortable, closed dysmenorrhea, asthenia cold abdominalgia, arthralgia pain due to rheumatism, injury from falling down, ulcer sores, dry constipation of intestines.《Sheng Nong's herbal classic》、《Wu Pu Book on Chinese herbal medicine》、《Mingyi Bielu》Deng on the books.Modern pharmacology research shows to mainly contain Ligustilide and its derivative, tonka-bean Plain class, flavonoids and organic acid.There is anti thrombotic action, improves hemorheology, improve coronary circulation, is anti-inflammatory, hypoglycemic, mending Blood effect etc..

Herba Epimedii：This product is Berberidaceae plant Herba Epimedii Epimedium brevicornu Maxim., Epimedium sagittatum Epimedium sagittatum (Sieb.et Zucc.) Maxim., E. Pubescens Epimedium pubescens Or the dried leaf of korean epimedium herb Epimedium koreamum Nakai Maxim..Property acrid, sweet, warm.Return liver and kidney channel.Function Filling liver kidney, strengthening the bones and muscles, wind-damp dispelling.For decline of kidney-YANG, impotence and seminal emission, muscles and bones impotence, arthralgia pain due to rheumatism, numbness contracture.First recorded in 《Sheng Nong's herbal classic》, multi-section Chinese medicine ancient books and records are on the books.Modern pharmacology research shows to mainly contain flavone compound, wood Quality, alkaloid etc..With function of resisting myocardial ischemia, antihypertensive effect, function of resisting osteoporosis, anti-aging, yang invigorating functions etc..

Morinda officinalis：This product is the dry root of madder wort Morinda officinalis Morinda officinalis How.Property is sweet, pungent, Tepor.Return kidney, Liver Channel.Function kidney-replenishing, strengthening the bones and muscles, wind-damp dispelling.For impotence and seminal emission, uterus cold infertile, irregular menstruation, abdomen is cold less Bitterly, arthralgia pain due to rheumatism, muscles and bones impotence.First recorded in《Sheng Nong's herbal classic》、《Bencao jizhu》、《Tang materia medica》、《Compendium of Materia Medica》Deng Chinese medicine ancient books and records are on the books.Modern pharmacology research shows to mainly contain Anthraquinones, polysaccharide, iridoid glycosides compound. In addition to filling liver kidney, outside strengthening the bones and muscles, also antidepression, improve cellular immune function, prevention and treatment of coronary heart disease reduces the works such as cholesterol With.

Teasel root：This product is the dry root of Dipsacaceae plant teasel Dipsacus asper Wall.ex Henry.Property It is bitter, pungent, tepor.Return liver and kidney channel.Function filling liver kidney, strengthening the bones and muscles, continuous injured, only uterine bleeding.For kidney deficiency and liver, soreness and weakness of waist and knees, wind Arthritis with fixed pain caused by dampness pain, injury from falling down, injury of tendon and muscle fracture, uterine bleeding, threatened abortion.First recorded in《Sheng Nong's herbal classic》, modern pharmacology research shows mainly Contain triterpene saponin, iridoid glycosides, alkaloid compound.To promoting union to have certain effect, also it is improved Immunity, anti-aging, it is anti-inflammatory the effects that.

Semen Cuscutae：This product is convolvulaceous plant Cuscuta australis Cuscuta australis R.Br. or Semen Cuscutae The dry mature seed of Cuscuta chinensis Lam..Property is pungent, sweet, flat.Return liver, kidney, the spleen channel.Function tonifies the liver and kidney, Gu Final minification is urinated, tocolysis, improving eyesight, antidiarrheal；External application disappears wind nti-freckle.For kidney deficiency and liver, soreness and weakness of waist and knees, impotence and seminal emission, enuresis frequent micturition, kidney Empty threatened abortion, pulsation is uneasy, and blurred vision tinnitus, spleen kidney deficiency is rushed down；Externally treating leucoderma.First recorded in《Sheng Nong's herbal classic》.Modern pharmacology is ground Study carefully and show to mainly contain flavone compound, has the effects that establishing-Yang, adjusts endocrine, anti-aging.

Hairyvein agrimony：This product is the dry aerial parts of rosaceous plant hairyvein agrimony Agrimonia pilosa Ledeb..Property It is bitter, puckery, it puts down.Converge to heart and liver channels.Function astringing to arrest bleeding, preventing malaria, stop dysentery, removing toxic substances, qi-restoratives.For spitting blood, haematemesis, metrostaxis, malaria Disease, bloody flux, carbuncle sore tumefacting virus, under pruritus vulvae band, de-power internal lesion caused by overexertion.First recorded in《Figure is through book on Chinese herbal medicine》, modern pharmacology research shows mainly to contain Have triterpene saponin, flavonoids, phenols, tannin and glucoside compound, have antitumor, hypoglycemic, easing pain and diminishing inflammation, hemostasis, The effects that antimalarial, anti-arrhythmia.

Rhizoma Atractylodis Macrocephalae：This product is the dry rhizome of feverfew Rhizoma Atractylodis Macrocephalae Atractylodes macrocephala Koidz..Property Bitter, sweet, warm.Returns spleen, stomach.Function strengthening the spleen and replenishing qi, eliminate dampness and have diuretic effect, hidroschesis, tocolysis.For spleen eating less, abdominal distention diarrhea, phlegm and retained fluid Feel dizzy and heart palpitations, oedema, spontaneous perspiration, fetal irritability.First recorded in《Sheng Nong's herbal classic》.Modern pharmacology research shows to mainly contain volatile oil And polysaccharide, the effects that stomach, enhancing immune function, antitumor, anti-aging can be adjusted.

Fructus amomi：This product is zingiberaceous plant amomum viosum Amomum villosum Lour., green shell sand Amomum villosum Lour.var.xanthioides T.L.Wu et Senjen's or SEMEN AMOMI LONGILIGULA Amomum longiligulare T.L.Wu is dry Dry ripening fruits.Property is acrid in flavour and warm in nature.Spleen, stomach, kidney channel.Function dampness elimination appetizing, warming spleen and stopping diarrha, regulating the flow of vital energy antiabortive.For turbi damp obstructing in middle-JIAO, gastral cavity Ruffian is not hungry, deficiency-cold in spleen and stomach, vomiting and diarrhea, pernicious vomiting, fetal irritability.First recorded in《Bencao Jingshu》.Modern pharmacology research table It is bright, volatile oil, saponins and flavone compound are mainly contained, has definite medicine to digestive system, immune system and nervous system Reason activity, also has anti-inflammatory, cholagogic and analgesic activity.

It is an advantage of the invention that：Using Chinese herbal medicine as raw material, better efficacy, compatibility is reasonable, collaboration plays a role, effect is bright Really, safety is without any side effects.

Beneficial effects of the present invention：

Though traditional Chinese medicine is without " hindering again " this name of disease, according to its clinical manifestation, acute aplastic anemia should belong to " acute pulmonary tuberculosis ", " marrow It is withered ", the row of " fever ", chronic aplastic anemia can belong to Chinese medicine " consumptive disease ", " deficiency of blood ", " blood trouble " scope.Chinese medicine is to the morbidity machine that hinders again System understanding is with a long history, and huge inspiration is given in the treatment to later age doctor.《Ling Shu Miraculous Pivot or Divine Axis is determined gas》Medium cloud：" the de- person's color of blood is white, dies young So not damp, arteries and veins is hollow, this its time.”《It is discussed in Plain Questions abdomen》It explicitly points out：" four limbs are clear, dizzy, frequent hematuria and hemafecia ... Name of disease blood depletion, this obtain it is young when, profuse bleeding ".The cause of disease and symptom to anaemia have made objective and lively description.《It is interior Through》" then product is empty at damage for consumption of vital essence in the body, and product damage is at labor for record.”《Lei Zheng Zhi Cai》Cloud " it is all it is deficient arise from taste, labor illness is a lot of in kidney Through ", illustrate this deficiency of five viscera be since consumption of vital essence in the body causes, and it is related with spleen kidney.The deficiency of vital energy then takes the photograph blood inability, and blood overflows outside arteries and veins；It is cloudy Empty then can cause in abnormal heat raw, injury of blood collaterals by heat compels the absurd row of blood, therefore has the diseases such as bleeding, fever.End of the Ming Dynasty analogy is prosperous, and consumptive disease (is hindered) again Three big symptom of clinic：Anaemia, bleeding, infection, which connect, to be described.He thinks " the card of consumptive disease《Gold is deficient》Chat in blood-arthralgia it Under, it is seen that its essence and blood of Lao Zebilao.Then interior heat rises ying blood wound, and five hearts are often warm ..., and the soft foot of bone is sour, and battalion's row day is slow, Wei Hang Disease, ying blood are compeled to defend gas, cannot in keep and emerge in outer, or spit or be defeated in battle or go out the key of two the moon, bleeding is both more, intimately spouts Enter, force decoct, it is unrestrained stop without body, ying blood have it is vertical to the greatest extent, how not wait for extremely " Yu Shi this section discussions, not only deeper into understanding Consumptive disease blood-arthralgia essence, and hinder this at all from the interpretation of the cause, onset and process of an illness essence and blood that links closely, it is indicated that generate heat for it is interior it is hot caused by and ying blood institute The multiple location bleeding syndrome of wound, if curing not in time, prognosis is very poor, therefore treatment answers nourishing generate fluid, blood-nourishing to hold back the moon, and beneficial to spirit and marrow is Method.

Though the starting of traditional Chinese medical herbal treatment blood disease is later, the progress and rapid progress to attract people's attention, Chinese medicine have been achieved There is notable curative effect to chronic aplastic anemia, curative effect is lasting, no obvious adverse reaction, hence it is evident that the effect of being better than simple western medical treatment.

The present invention is giving full play to traditional traditional Chinese medicine resources advantage, using guidance of traditional Chinese medicine theory and clinical practice, For a variety of causes for causing alpastic anemia, with red ginseng, Radix Paeoniae Alba, Radix Astragali, prepared rehmannia root, Radix Angelicae Sinensis, Herba Epimedii, Morinda officinalis, Teasel root, Semen Cuscutae, hairyvein agrimony, rhizoma atractylodis macrocephalae, fructus amomi are bulk pharmaceutical chemicals, are processed into through modern crafts.Red ginseng, Radix Paeoniae Alba are monarch in side Medicine, red ginseng can supplementing qi and nourishing yin promote the production of body fluid, Radix Paeoniae Alba then can blood-nourishing hold back the moon, two medicines have the work(of nourishing generate fluid with using.Radix Astragali can QI invigorating support Blood, Radix Angelicae Sinensis, prepared rehmannia root, hairyvein agrimony can replenishing and activating blood, hemostasis, four medicines share the benefits of can enhancing nourishing qi and blood, are ministerial drug in side. Herba Epimedii, Morinda officinalis, Semen Cuscutae, teasel root, four medicines share the work(for having strong marrow green blood, strengthening yang and invigorating kidney, are also ministerial drug in side.It fries white Art can strengthening the spleen and replenishing qi, fructus amomi can dampness elimination promoting the circulation of qi, two medicines with can good efficacy to suffering from a deficiency of the kidney, enable transporting and transforming function of the spleen and stomach function normal operation, and Taste are the source of vital function day after tomorrow, therefore two medicine compatibility applications are conducive to the recovery of qi and blood, are adjutant altogether in side.In short, in side The effect of all medicines, compatibility is precise and appropriate, plays nourishing generate fluid altogether, strong marrow green blood, strengthening yang and invigorating kidney, good efficacy to suffering from a deficiency of the kidney, reaches treatment aregeneratory Property anaemia, myelodysplastic syndrome, thrombopenia and chemicotherapy cause the purpose of cytopenia, made using the modern times Agent technique is made under rational method of quality control and stringent management of product measure.It is its stable quality, convenient to take. This product is safe and effective, can take for a long time, is alpastic anemia, myelodysplastic syndrome, thrombopenia and puts Chemotherapy causes the ideal drug of cytopenia patient.

By retrieving Chinese patent and bibliographic data base, have no and the same or similar patent of prescription of the present invention and research text Offer, prescription design theory of the present invention and Thinking Creation, based on prescription of the present invention exploitation have treatment alpastic anemia, Myelodysplastic syndrome, thrombopenia and chemicotherapy cause the Chinese patent drug of cytopenia, the market demand to have a extensive future.

Invention formulation main technique research is as follows：

One, water decocts technical study

1.1 Orthogonal Experiment and Design：12 taste medicine of this product uses water boiling and extraction, this time test determine investigate following 3 because Element is shown in Table 1.

1 water of table decocts technological factor water-glass

The characteristics of according to uniform design, carries out orthogonal test arrangement, is shown in Table 2.

2 L of table₉(3⁴) orthogonal test table

1.2 test method

The 12 taste medicinal material such as Radix Paeoniae Alba, Radix Astragali, prepared rehmannia root is weighed in prescription ratio, amounts to 140g, totally 9 parts, random compile is 1- 9 tested numbers are tested by experiment serial number, and extracting solution filtration, filtrate is concentrated into 200ml.

1.3 paste-forming rates measure

Precision draws 1~No. 9 sample liquid 25ml, is placed in and has dried into the evaporating dish of constant weight, water bath method sets 80 DEG C of bakings It is dried in case to constant weight, calculates paste-forming rate, the results are shown in Table 3.

1.4 paeoniflorin contents measure

Chromatographic condition is filler with octadecylsilane chemically bonded silica with system suitability；With -0.1% phosphorus of acetonitrile Acid solution (15:85) it is mobile phase；Detection wavelength is 230nm.

The preparation of reference substance solution takes Paeoniflorin reference substance appropriate, accurately weighed, adds methanol that every 1ml is made molten containing 30 μ g Liquid to get.

It is prepared by test sample：It is appropriate that precision weighs 9 parts of samples, adds methanol 25ml, is ultrasonically treated 40 minutes, and filtration, filtrate is steamed Dry, residue adds water 20ml to make dissolving, with water saturated n-butanol shaking extraction 4 times, each 20ml, merges n-butanol liquid, water-bath It is evaporated, residue adds methanol to make dissolving in right amount, and is transferred in 10ml measuring bottles, adds methanol dilution to scale, shakes up, and filtration takes continuous Filtrate to get.

Assay method：It is accurate respectively to draw reference substance solution and each 10 μ l of test solution, liquid chromatograph is injected, is surveyed It is fixed to get.

3 orthogonal experiments of table

* 0.05 * * P ＜ 0.01 of P ＜

4 Paeoniflorin analysis of variance table F of table_0.05(2.2)=19 F_0.01(2.2)=99

1.5 interpretation of result

Radix Paeoniae Alba is monarch drug in a prescription in side, and main component is Paeoniflorin, it is thus determined that its paeoniflorin content is main evaluation index； Though paste-forming rate cannot reflect the quality of preparation process completely, its height still has certain meaning to producing greatly, thus by its As auxiliary evaluation index.

1.6 intuitive analyses：

From paeoniflorin content intuitively analyze as can be seen that decoct number have larger impact to Paeoniflorin unit content, other Factor influence is smaller, and can be seen that each factor influences order from R values is A ＞ B ＞ C, optimised process A₂B₂C₃, i.e.,：It adds water to cook It is secondary, 1.5 hours every time, add 10 times of amounts of water.

From paste-forming rate intuitively analyze as can be seen that decoct number have larger impact to paste-forming rate, from R values can be seen that it is each because It is A ＞ B ＞ C, optimised process A that element, which influences order,₃B₃C₃, that is, add water to cook three times, 2 hours every time, add 10 times of amounts of water.

1.7 variance analysis：

Variance analysis is carried out by inspection target of Paeoniflorin unit content, can be seen that extraction time to Chinese herbaceous peony from upper table 4 Medicine glycosides unit content has highly significant influence, and extraction time, without conspicuousness influence, it was A ＞ B ＞ to influence order with water multiple is added C；It is consistent with intuitive analysis.

Comprehensive analysis, wherein extraction time (A) have highly significant influence, extraction time (B) He Jiashui to paeoniflorin content Multiple (C) does not make significant difference to paeoniflorin content；And paste-forming rate is not the bigger the better in actual production, in conjunction with industrial big production Actual conditions and production need, energy saving, shortening extraction time, while reducing impurity proposition amount, and 3 factors are determined as： It adds water to cook 2 times, 1.5 hours for the first time, adds 8 times of amount water；Second 1.0 hours, add 6 times of amount water.

Two, concentration technology is investigated

To prevent in this prescription active ingredient is heated for a long time to damage, is concentrated, had using triple effect pressure regulating equipment Concrete conditions in the establishment of a specific crime is：80 DEG C, vacuum degree is 0.08~0.1MPa, is concentrated into the clear cream that relative density is 1.20~1.25 (60 DEG C).

Three, the investigation of drying process

In order to effectively control the total amount of finished product, ensure between batches consistent, extracting solution, which is concentrated into relative density, is The clear cream of 1.20~1.25 (60 DEG C), 70 DEG C are dried under reduced pressure, and vacuum degree is 0.08~0.1MPa.

Four, it extracts, concentrate, drying process verification

According to determining extraction process route and concentration, drying parameter, three batches of lab scale verifications are carried out, in prescription ratio It feeds intake, dry cream amount is made, calculate dry cream rate, paeoniflorin content, the results are shown in Table 5.

5 three batches of extractions of table, concentration, drying process verification result

Test result shows that three batches of lab scales verification paste-forming rates and paeoniflorin content are more consistent, illustrate the extraction, concentration, Drying process is stablized feasible.

Five, Study on Forming

The selection of 5.1 supplementary product consumptions

In order to adapt to the medication demand of all kinds of patients, the quasi- exploitation of this product is granule, therefore auxiliary material selects dextrin and sucrose. The dosage of States Pharmacopoeia specifications granule auxiliary material is usually no more than 2 times of dry cream amount, and the proportioning of auxiliary material and dry extract is investigated in this time experiment. 3 parts of extract powder is taken, respectively according to extract powder and auxiliary material 1:1、1:1.5、1:Dextrin and cane sugar powder is added in 2 ratio, selects 80% Ethyl alcohol is wetting agent, is pelletized, dry, whole grain.Granularity is screened using standard screen, and tastes mouthfeel, the results are shown in Table 6,7.

6 diluent dosage of table

7 diluent dosage test result of table

As a result No. 1 particle, the summation that (10 mesh) and the particle that can sieve (80 mesh) by No. five cannot be sieved by No.1 are big In 15%, regulation of the Chinese Pharmacopoeia version four in 2015 to granule granularity is not met, the granularity of No. 2 and No. 3 particles then meets Regulation.

No. 2 and No. 3 particle 10g are taken respectively, it is molten by being checked under Chinese Pharmacopoeia four rules of preparations granule items of version in 2015 Change property method inspection, as a result No. 2 and No. 3 particle meltings it is good, particle all dissolves.

The result shows that when extract powder and auxiliary material are 1:1.5 and 1:When 2, granule obtained meets Chinese Pharmacopoeia 2015 Related every regulation under four rules of preparations granule items of version.In view of reducing supplementary product consumption as far as possible, therefore, by supplementary product consumption It is set to：Extract powder：Dextrin：Sucrose (1:0.5:1).

The selection of 5.2 concentration of wetting agent

It is wetting agent to select the ethyl alcohol of 70%, 80% and 90% concentration respectively, carries out experimental study.In pelletization, When with 70% ethyl alcohol softwood, stickiness is larger, and softwood is easy caking and blocks sieve, when with 90% ethyl alcohol softwood, obtained Grain fine powder is more, and ratio of briquetting is relatively low, and particle is loose, and when with 80% ethyl alcohol softwood, particle size distribution is more uniform, and hardness is suitable In, therefore, wetting agent is set to 80% ethyl alcohol.

5.3 mobility are investigated

Its angle of repose is measured using fixed funnel method to the particle made by above-mentioned condition.Take the surface plate of a diameter of 8cm The center of circle delimited in the back side, funnel be fixed on holder, the center of lower end face surface plate, high 30cm takes particle to make it from leakage It is flowed down naturally on bucket, until until tapered peripheral to surface plate edge, measures cone height H, calculate angle of repose a=ArctgH/R. It the results are shown in Table 8.

8 angle of repose measurement result of table

As a result the angle of repose of particle illustrates the good fluidity of particle at 30 ° or so.

Six, preparation process summarizes

In summary：Above 12 taste adds water to cook secondary, 1.5 hours first times, and 8 times of water is added to measure, second 1 hour, 6 times of amounts of water, collecting decoction are added to filter, filtrate measures the clear cream that relative density is 1.20-1.25 when being condensed into 60 DEG C, 70 DEG C subtract Pressure is dried, crushed into fine powder；Take extract powder, dextrin, cane sugar powder by 1:0.5:Particle is made with 80% ethyl alcohol in 1 ratio mixing, It is dry, whole grain to get.

Seven, the yield and primary quality measure of three batches of small trial products

According to ginseng Chinese herbaceous peony nourishing qi and blood particle process testing parameter, feeds intake in recipe quantity ratio and carried out the three batches of lab scale samples Production, as a result yield substantially constant, primary quality measure meet pertinent regulations under granule item, the results are shown in Table 9.

The yield and primary quality measure of 9 three batches of small trial products of table

Eight, the determination of packing specification

Packing specification：According to actual conditions, it is determined as per packed 10g.

Nine, the yield and primary quality measure of three batches of pilot products

The yield and primary quality measure of 10 3 batches of pilot products of table

Conclusion：Production technology of the present invention is scientific and reasonable, remains the proposition of the active ingredient of drug to greatest extent, ensures Drug quality is stablized.

The present invention joins the acute toxicity test of Chinese herbaceous peony nourishing qi and blood particle, pharmacodynamics test and is compared with the prior art experiment such as Under：

One, present invention ginseng Chinese herbaceous peony nourishing qi and blood particle acute toxicity test

1. test objective

Acute toxic reaction caused by the ginseng Chinese herbaceous peony nourishing qi and blood particle of intragastric administration on mice large dosage is observed, LD is measured₅₀Or most Big dosage.The toxic reaction being likely to occur when the possible target organ of analysis dealed with medicine went and people's overdose drug.

2. test material

2.1 experimental animal：Kun Ming mice, half male and half female, 18~22g of weight, animal quality quality certification number NO： 0014961, it is provided by The Fourth Military Medical University (being now renamed as air force surgeon university) Experimental Animal Center, animal productiong licensing： SCXK (army) 2012-0007.Rearing conditions：Shaanxi TCM Universities herbal pharmacology laboratory (State Administration of Traditional Chinese Medicine's traditional Chinese medicine Scientific research three-level laboratory), feed is small white mouse full nutrition feed.

2.2 test medicine：Join Chinese herbaceous peony nourishing qi and blood granule medicament extract powder (per g extract powders crude drug containing 6.67g), by Shaanxi Hao Its Pharmacy stock Co., Ltd of army provides, lot number 20171001.Join Chinese herbaceous peony nourishing qi and blood particle by red ginseng, Radix Paeoniae Alba, Radix Astragali, radix rehmanniae preparata Huang, Radix Angelicae Sinensis, Herba Epimedii, Morinda officinalis, teasel root, Semen Cuscutae, hairyvein agrimony, rhizoma atractylodis macrocephalae, fructus amomi composition have nourishing generate fluid, strong marrow life The effect of blood, strengthening yang and invigorating kidney, good efficacy to suffering from a deficiency of the kidney.Subtract for treating alpastic anemia, myelodysplastic syndrome, blood platelet Few disease and chemicotherapy cause cytopenia.Card is shown in qi-blood deficiency, the insufficiency of the kidney yang.Usage and dosage：Boiling water is taken after mixing it with water, once a bag (10g is filled, and is equivalent to 25.2g crude drugs), 3 times a day, i.e., clinical daily people's dosage is that (weight of people is with 60kg by 1.26g crude drugs/kg Meter).When experiment, drug extract powder is made into the suspension (889%, i.e. maximum concentration) of every ml crude drugs containing 8.89g with pure water, It is shaken up when administration.

3. test method

(1) trial test：The Kunming mouse 5 of fasting 12h is taken, male and female are used, and the ginseng Chinese herbaceous peony QI invigorating that gavage gives 889% is supported Blood granule medicament extract powder suspension (maximum concentration) 40ml/kg, unit dosage form are 355.6g crude drugs/kg, are observed, as a result It is showed no acute toxic reaction and death, carries out maximum dosage-feeding measurement then.

(2) maximum dosage-feeding measures：Take the Kunming mouse 40 of fasting 12h, half male and half female, be randomly divided into control group and Join Chinese herbaceous peony nourishing qi and blood particle group (hereinafter referred to as administration group).Administration group mouse joined Chinese herbaceous peony nourishing qi and blood every 4 hours gavages 889% Grain drug extract powder suspension (maximum concentration) 40ml/kg, be administered altogether it is secondary, i.e., accumulated dose be 711.2 crude drugs/kg.Control group Give the pure water of equivalent capability.Free diet after administration, observe and record daily Central Nervous System of Mouse, breathing and angiocarpy, The indexs such as gastronintestinal system, urogenital system, skin and hair, eyes, weight, continuous 14 days.After observation expires, weigh in, Animal is put to death in dislocation, and substantially become celestial observation, and the important organs such as coring, liver, spleen, lung, kidney are fixed with 10% formalin, Conventional materials, dehydration, waxdip, embedding, slice, HE are dyed, and pathological examination is carried out under light microscopic.

4. observation index

In terms of nervous system：1. behavior and reaction include abnormal cry, agitation, uneasiness, irritability, hyperesthesia, less move, Drowsiness or stupor etc.；2. movement includes jerk, stiff, forced movement, relaxation, paralysis etc.；3. pupil and secretion include Pupil whether zooms in or out, and stream birth is shed tears.

Breathing and angiocarpy：Including being short of breath or the secretion of too slow, nose, pareordia heart rate speed etc. is touched.

Gastronintestinal system：Including abdomen flatulence or contraction, hard, wetter, shapeless or melena, the ashen of defecating are just etc..

Urogenital system：Including labia, mammary gland swelling, perineum dirt etc..

Skin and hair：Including color, integrality, whether it is congested, cyanosis is pale, dermexanthesis, fur are loose etc..

Eyes：Ptosis, is trembled at exophthalmos.

Weight：It is weighed before administration and after being administered 7,14 days

5. result

Every time after administration, mouse moves administration group less, restores normal after 1h.Stool is in aterrimus after most of mouse administrations, It is wetter soft, restore after 24 hours normal.In other observing times, administration group animal appearance sign no abnormality seen, activity freely, into Food, drinking-water, excrement are normal, and fur is bright and clean, and mouth, nose, eye are without secretion, pudendum cleaning.Administration group mouse weight and control group Comparing difference does not occur other acute toxic reaction symptoms and death without significant (being shown in Table 1) yet.Animal postmortem is put to death, Thoracic cavity, abdominal cavity no abnormality seen liquid, the main organs such as the heart, liver, spleen, lung, kidney have no color, paramophia, bleeding, oedema or its Its pathological change；Gastric mucosa is without bleeding or ulcer, and intestinal tube has no flatulence, compared with the control group no significant difference.

The variation of 1 acute toxicity test the weight of animals of table (N=20)

The organs and tissues such as the heart, liver, spleen, lung, kidney are won, 10% formalin is fixed, routine pathology film-making, is carried out under light microscopic Histopathological examination.The result shows that the visible heart wall of administration group mouse heart is clear in structure, myocardial ultramicrostructure is complete, has no It is abnormal.The visible lobuli hepatis structure of administration group mouse liver is complete, liver cell marshalling, and cholangiole and bile capillaries have no different Often；The visible spleen essence white pulp of administration group mouse spleen and red pulp institutional framework understand, have no lymphocyte paraplasm and reduction； The visible lung's steep that wall capillary of administration group mouse lung is normal, and most of chamber that steeps is full of pale red, and the profile of alveolar indistinctly may be used See, no abnormality seen；The visible cortex renis of administration group mouse kidney and medulla tissue structure understand that the nephron is evenly distributed, glomerulus Blood capillary tube bank, capsular epithelium cell no abnormality seen pathological change, renal interstitial is without blood vessel dilatation, inflammatory exudation, mucous membrane of renal pelvis Transitional epithelium, no squamous metaplasia, no inflammation cellular infiltration, no kidney envelope thicken, no abnormality seen；Administration group Mouse Stomach is visible Four layers of gastric mucosa, submucosa, muscle layer and outer membrane, structural integrity, no abnormality seen.Administration group mouse core, liver, spleen, lung, kidney, No significant difference compared with the control group of the organs and tissues such as stomach histopathological examination (see Figure of description 1-12).

6. conclusion

The maximum dosage-feeding that the present invention joins Chinese herbaceous peony nourishing qi and blood particle intragastric administration on mice is 711.2g crude drugs/kg, is equivalent to clinic 564.4 times (daily dosage is 1.26g crude drugs/kg, and the weight of people is in terms of 60kg) of people's dosage, gavage is to mouse at this dose Without acute toxic reaction.In view of ginseng Chinese herbaceous peony nourishing qi and blood particle prescription nontoxicity medicinal material, the incompatibility such as no eighteen incompatibilities, nineteen counteraction, Think in conjunction with this test result, ginseng Chinese herbaceous peony nourishing qi and blood particle will not cause acute toxic reaction in clinical usage and dosage.

Bibliography

1. state food pharmaceuticals administration general bureau《Drug single-dose toxicity research technological guidance's principle》.2014 year 5 The moon 13

2. Qi Chen edits《Herbal pharmacology research methodology》3rd edition, Beijing:People's Health Publisher, in December, 2011

Two, present invention ginseng Chinese herbaceous peony nourishing qi and blood particle pharmacodynamics test

(1) present invention ginseng Chinese herbaceous peony nourishing qi and blood particle pair⁶⁰The treatment of Co- γ joint cyclophosphamide induced aplastic anemias Effect

【Abstract】Purpose observation ginseng Chinese herbaceous peony nourishing qi and blood particle pair⁶⁰Co- γ joint cyclophosphamide causes BalB/C mouse regeneration barrier The therapeutic effect of impenetrability anaemia.Method takes male BalB/C mouse 72, takes 12 to be only used as normal group at random, remaining animal is used 3.0Gy ⁶⁰Co- gamma Rays 4 divide 51 seconds, 4d, 5d, 6d intraperitoneal injection of cyclophosphamide 40mg/kg after radiation, reproduction Aplastic anemia model.To be divided at mould mouse model group, ginseng Chinese herbaceous peony nourishing qi and blood particle it is small, in, large dosage of group (1.26g/kg, 7.56g/kg, 15.12g/kg) and Yixiesheng capsule group (0.625g contents/kg).Each group continuous gavage is administered one week, observation Animal general signs carry out power and exhaust experiment for 12 hours before last dose.1 hour after the last administration, each group eyeball of mouse took blood, into Row hematological examination；Blood film carries out reticulocyte count percentage；Slide method measures the clotting time；Animal is put to death, is won Femur prepares bone marrow suspension with marrow fliud flushing (3.8% sodium citrate: PBS=1: 9 prepare) flushing, and direct counting method measures bone Marrow karyocyte quantity.As a result ginseng Chinese herbaceous peony nourishing qi and blood particle large dosage group has different degrees of change to animal pattern general signs It is kind, it can extend that power exhausts distance and power exhausts the time (P ＜ 0.05 or P ＜ 0.01)；Large dosage group mouse WBC, PLT, granulophilocyte It is significantly increased compared with model group (P ＜ 0.01)；Join Chinese herbaceous peony nourishing qi and blood particle it is small, in, large dosage of group can be to some extent compared with model group Shorten the clotting time, increase bonemarrow nucleated cells number (P ＜ 0.05 or P ＜ 0.01).Conclusion ginseng Chinese herbaceous peony nourishing qi and blood particle 7.56, 15.12g crude drugs/kg pairs⁶⁰Co- γ joint cyclophosphamide causes BalB/C aplastic anemia in mice to have different degrees for the treatment of to make With, and the bigger effect of dosage is more apparent.

1. test objective

It is main performance that alpastic anemia (aplastic anemia, AA referred to as hinder again), which is with whole blood trace elements, One group of syndrome, pathogenic factor is complicated, and radioactive ray are one of pathogenic factors.This experiment uses⁶⁰Co- γ joint cyclophosphamide causes Alpastic anemia BalB/C mouse models, by detecting peripheral white blood cells (white blood cell, WBC), red thin Hematological examinations, the nets such as born of the same parents (red blood cell, RBC), hemoglobin (HGB), blood platelet (Platelets, PLT) are knitted Red blood cell number, bonemarrow nucleated cells number, clotting time, general signs, power exhaust the indexs such as time and distance, and evaluation ginseng Chinese herbaceous peony QI invigorating is supported Blood particle and the relevant Pharmacodynamics of major function.

2. materials and methods

2.1 drug and reagent：Join Chinese herbaceous peony nourishing qi and blood particle (containing crude drug 2.52g per g particles), by its army's pharmacy stock of Shaanxi Hao The production of part Co., Ltd, lot number：20171001.Join Chinese herbaceous peony nourishing qi and blood particle by red ginseng, Radix Paeoniae Alba, Radix Astragali, prepared rehmannia root, Radix Angelicae Sinensis, excessive The sheep leaves of pulse plants, Morinda officinalis, teasel root, Semen Cuscutae, hairyvein agrimony, Rhizoma Atractylodis Macrocephalae (stir-fry), fructus amomi composition, there is nourishing generate fluid, blood-nourishing to hold back the moon, strengthening the essence is filled out Marrow and other effects, for dizzy weak, pale, palpitation, insomnia and dreamful sleep, bleeding etc. caused by aplastic amenia Disease；It is 1.26g crude drugs/kg through converting clinical people daily with dosage (weight of people is in terms of 60kg).(traditional Chinese medicines are accurate for Yixiesheng capsule Word Z19893056, donkey-hide gelatin, colla carapacis et plastri testudinis, deer horn glue, deer blood, ox marrow, dried human placenta, pilose antler, Poria cocos, Radix Astragali, Radix Paeoniae Alba, Radix Angelicae Sinensis, party Ginseng, prepared rehmannia root, Rhizoma Atractylodis Macrocephalae, prepared fleece flower root, jujube, stir-baked FRUCTUS CRATAEGI, coloured malt, Endothelium Corneum Gigeriae Galli (parched), rhizoma anemarrhenae, rheum officinale, peanut coat composition, have The effect of strengthening spleen, tonifying kidney, green blood strengthening the essence, it to be used for speen-kidney dificiency, the lusterless complexion caused by asthenia of essence and blood, dizziness shortness of breath is used for iron deficiency Property the diseases such as anaemia, chronic aplastic anemia, clinical people daily is 3g contents with dosage), health medicine is answered by Jilin three nine-day periods after the winter solstice gold Industry Co., Ltd produces, lot number 170806.It is limited to contain enlightening medicine by Jiangsu for syklofosfamid ampoule (0.2g/ bottles of specification, CTX) Company produces, lot number 17110425.

2.2 experimental animal：BalB/C mouse, male, 6~8 week old, 25~30g of weight, feed are sought for small white mouse full price Feed is supported, (is now renamed as by The Fourth Military Medical University：Air force surgeon university) Experimental Animal Center offer, animal quality quality certification number NO：0014962, production licence number：SCXK (army) 2012-0007.Rearing conditions：Shaanxi university of TCM herbal pharmacology is real Test room (State Administration of Traditional Chinese Medicine's traditional Chinese medicine scientific research three-level laboratory).

2.3 key instrument equipment：BC-5300Vet type fully automatic blood cytoanalyzes, Shenzhen step auspicious biologic medical electricity JIANMEI ELECTRONICS CO LTD produces；FT-200 type animal treadmills, Chengdu TME Technology Co., Ltd.'s production；E100 type inverted lights Microscope, Nikon Corporation production.

2.4 modeling^[1、2], grouping and administration：Male BalB/C mouse 72 are taken, take 12 to be only used as normal group at random, remaining Animal sets 3.0Gy⁶⁰Under Co- gamma-rays, 4 points are radiated 51 seconds with dosage rate 1.3531Gy/min, distance 70cm, the after radiation 4,5,6d distinguishes intraperitoneal injection of cyclophosphamide 40mg/kg, replicates⁶⁰Co- γ joint cyclophosphamide induced aplastic anemias BalB/ C mouse models (AA models).7d will at mould mouse by weight be randomly divided into model group, ginseng Chinese herbaceous peony nourishing qi and blood particle it is small, In, large dosage of group (1.26,7.56,15.12g crude drugs/kg, be equivalent to 1,6,12 times of people's dosage, it is hereinafter referred to as small, in, it is big Dosage group) and Yixiesheng capsule group (0.625g contents/kg are equivalent to 12 times of quantity, referred to as beneficial blood life group).Each group Daily gastric infusion is primary, continuous 7d.The physiological saline of normal group and the daily gavage isometric(al) of model group.

2.5 Testing index and method^[3~6]

2.5.1 general signs：It observes each group mouse cerebration, diet, fur, development, two just and death etc. is general Sign.Each group mouse is singly only placed on animal treadmill by last dose the previous day, and in the case where rotating speed is 35r/min, running is until power It exhausts, record power exhausts the time and power exhausts distance.

2.5.2 hematological examination and reticulocyte count：1 hour after the last administration, eyeball took blood, carried out hematology inspection It looks into.Simultaneously take blood 1 drop be placed in 2mL EP pipes, be added two drop brilliant cresyl blue dye liquors, shake up, stand 15min after, draw 10 μ l in On glass slide, push jack counts the percentage of granulophilocyte under microscope.

2.5.3 the clotting time：It takes blood to set on slide, the clotting time is measured with slide method.

2.5.4 bonemarrow nucleated cells number mesh：Animal is put to death after taking blood, detaches femur, exposure both ends marrow, with marrow fliud flushing (3.8% sodium citrate: PBS=1: 9) 1mL rinses ossis repeatedly, obtains bone marrow suspension.Take 1 droplet of bone marrow suspension in cytometer On number plate, the karyocyte number in each visual field is directly read under inverted microscope, calculates bonemarrow nucleated cells number.

2.6 data statistics：Data analysis is carried out with SPSS 24.0, data are usedIt indicates, is compared with model group Compared with 0.05 differences of P ＜ with statistical significance.

3. result

The influence of 3.1 pairs of general signs：Normal group mouse reaction is quick, activity flexibly, fur gloss, diet, development with And two just normal, no death.The activity of model group mouse, diet are reduced, and the state of mind is bad, and soft stools, fur is holded up, and disappears Thin, weight mitigates, and ear, lip, first portion are pale, is in anaemia looks, there is 4 death.Join the large, medium and small dosage of Chinese herbaceous peony nourishing qi and blood particle Each dead 3 of group, compared with model group, ginseng Chinese herbaceous peony nourishing qi and blood particle is big, middle dosage has general signs different degrees of improvement. Ginseng Chinese herbaceous peony nourishing qi and blood particle is big, middle dose group mouse power exhausts distance and power exhausts the time and significantly extends (P ＜ 0.05 compared with model group Or P ＜ 0.01, table 1).Prompt：The general signs that ginseng Chinese herbaceous peony nourishing qi and blood particle is big, middle dosage can improve AA mouse.

Table 1 exhausts distance to AA mouse power and power exhausts the influence of time

Note：Compared with model group, * P ＜ 0.05, * * P ＜ 0.01

3.2 right⁶⁰Co- gamma-rays and CTX cause AA mouse peripheral bloods as and granulophilocyte influence (table 2)

Table 2 to AA mouse peripheral bloods as and granulophilocyte influence

Note：Compared with model group, * P ＜ 0.05, * * P ＜ 0.01

The result shows that model group mouse peripheral blood WBC, RBC, HGB, PLT, reticulocyte counts more normally organize significant decrease (P ＜ 0.05 or P ＜ 0.01), shows some features hindered again, illustrates modeling success.Join Chinese herbaceous peony nourishing qi and blood particle large dosage Group mouse WBC, PLT, granulophilocyte significantly increase (P ＜ 0.01) compared with model group, prompt ginseng Chinese herbaceous peony nourishing qi and blood particle large dosage AA mouse can be improved.

The influence (table 2) of 3.3 pairs of AA BalB/C clotting time of mice and bone marrow nucleated cell

Influence of the table 2 to AA BalB/C clotting time of mice and bonemarrow nucleated cells number

Note：Compared with model group, * P ＜ 0.05, * * P ＜ 0.01

The result shows that more normal group of model group clotting time of mice is obviously prolonged (P ＜ 0.01), bonemarrow nucleated cells number is shown It writes and reduces (P ＜ 0.01), illustrate that model group mouse shows blood coagulation disorders, medulla hematopoietic system suppression, and hinder clinic again Feature is consistent, illustrates modeling success.Join Chinese herbaceous peony nourishing qi and blood particle it is small, in, large dosage of group (1.26,7.56,15.12g crude drugs/kg) It can shorten the clotting time to some extent compared with model group, increase bonemarrow nucleated cells number (P ＜ 0.05 or P ＜ 0.01).Prompt：Ginseng Chinese herbaceous peony nourishing qi and blood particle can improve the bleeding and bone marrow suppression for hindering that mouse is likely to occur again.

4. conclusion

Alpastic anemia be the marrow hemopoietic stem cells defect caused by many reasons, hematopoieticmicroenviron-ment damage and Immunologic mechanism changes, and leads to marrow hematopoiesis function failure, occurs with whole blood trace elements being the disease mainly showed, pathogenic factor Complexity, radioactive ray are one of pathogenic factors.This experiment uses⁶⁰Co- γ joint cyclophosphamide induced aplastic anemias BalB/C Mouse model, the model is consistent with some clinical manifestations hindered again, is easy, favorable reproducibility animal model.Experiment passes through inspection The indexs such as peripheral hemogram, reticulocyte counts, bonemarrow nucleated cells number, clotting time, general signs are surveyed, evaluation ginseng Chinese herbaceous peony QI invigorating is supported Blood particle and the relevant Pharmacodynamics of major function.The result shows that the present invention joins in Chinese herbaceous peony nourishing qi and blood particle, large dosage of group The These parameters that (7.56,15.12g crude drugs/kg) can improve AA model mices to some extent are abnormal, have certain treatment Effect.

(2) present invention ginseng Chinese herbaceous peony nourishing qi and blood particle causes the prevention effect that mouse bone marrow cells inhibit to 5 FU 5 fluorouracil

Abstract：Purpose observation ginseng Chinese herbaceous peony nourishing qi and blood particle causes the prevention effect that mouse bone marrow cells inhibit to 5 FU 5 fluorouracil.Side Method takes male mice in kunming, be randomly divided into normal group, model group, ginseng Chinese herbaceous peony nourishing qi and blood particle it is small, in, large dosage of group (1.26, 7.56,15.12g crude drugs/kg is equivalent to 1,6,12 times of people's dosage), Yixiesheng capsule group (0.625g contents/kg, phase When in 12.5 times of quantity), each group gastric infusion, once a day, successive administration 12d.Normal group and model group fill daily The isometric physiological saline of stomach.4d is administered in addition to normal group, 5 FU 5 fluorouracil 125mg/ is injected intraperitoneally in other each group mouse Kg, in administration 6d and 8d, intraperitoneal injection 5 FU 5 fluorouracil 62.5mg/kg is primary respectively again.1 hour after the last administration, into Row hematological examination, detection reticulocyte counts, clotting time and bonemarrow nucleated cells number etc..As a result join Chinese herbaceous peony nourishing qi and blood particle Large dosage group mouse WBC, PLT, granulophilocyte significantly increase (P ＜ 0.01) compared with model group, in ginseng Chinese herbaceous peony nourishing qi and blood particle, Large dosage of group can shorten the clotting time compared with model group, increase bonemarrow nucleated cells number (P ＜ 0.05 or P ＜ to some extent 0.01).Conclusion present invention ginseng Chinese herbaceous peony nourishing qi and blood particle 15.12g crude drugs/kg energy elevation model mouse WBC, PLT, net are knitted red thin Born of the same parents, bonemarrow nucleated cells number shorten the clotting time, cause chmice acute bone marrow suppression to have prevention effect 5 FU 5 fluorouracil.

1. test objective：Observation ginseng Chinese herbaceous peony nourishing qi and blood particle causes the prevention effect that mouse bone marrow cells inhibit to 5 FU 5 fluorouracil.

2. materials and methods

2.1 drugs and reagent：Injection 5 FU 5 fluorouracil (specification 250mg：10mL), limited by the general medicine company in the rising sun East Sea, Shanghai Company produces, lot number：FA170603；Remaining is the same as experiment one.

2.2 experimental animal：Kunming mouse, male, weight 25-30g, feed are small white mouse full nutrition feed, by The Fourth Military Medical University (is now renamed as：Air force surgeon university) Experimental Animal Center offer, animal quality quality certification number NO： 0014963, production licence number：SCXK (army) 2012-0007.Rearing conditions are the same as experiment one.

2.3 key instrument equipment：With experiment one.

2.4. method^[7~9]：Male mice in kunming is taken, normal group is randomly divided into, model group, joins Chinese herbaceous peony nourishing qi and blood particle It is small, in, large dosage of group (1.26,7.56,15.12g crude drugs/kg, be equivalent to 1,6,12 times of people's dosage), Yixiesheng capsule group (0.625g contents/kg are equivalent to 12.5 times of quantity), each group gastric infusion, once a day, successive administration 12d. The physiological saline of normal group and the daily gavage isometric(al) of model group.4d is administered in addition to normal group, other equal abdominal cavities of each group mouse 5 FU 5 fluorouracil 125mg/kg is injected, 5 FU 5 fluorouracil 62.5mg/kg mono- is injected intraperitoneally respectively again in administration 6d and 8d It is secondary.1 hour after the last administration, hematological examination, detection reticulocyte counts, clotting time are carried out by one method and steps of experiment And bonemarrow nucleated cells number.

2.5 data statistics：With experiment one.

3. result

3.1 pairs of 5 FU 5 fluorouracils cause bone marrow suppression mouse peripheral bloods as and reticulocyte counts influence (table 1)

Table 1 to 5 FU 5 fluorouracil cause bone marrow suppression mouse peripheral blood as and granulophilocyte percentage influence

Note：Compared with model group, * P ＜ 0.05, * * P ＜ 0.01

The result shows that model group mouse peripheral blood WBC, PLT, reticulocyte counts more normally organize significant decrease (P ＜ 0.01) feature for, showing Acute Meyloid inhibition illustrates modeling success.Ginseng Chinese herbaceous peony nourishing qi and blood particle large dosage group mouse WBC, PLT, granulophilocyte significantly increase (P ＜ 0.01) compared with model group, prompt ginseng Chinese herbaceous peony nourishing qi and blood particle large dosage that can improve mould The Acute Meyloid of type mouse inhibits.

3.2 pairs of 5 FU 5 fluorouracils cause bone marrow suppression mouse bone marrow cells number of nucleated cells and the influence in clotting time (table 2)

Table 2 causes bone marrow suppression mouse bone marrow cells number of nucleated cells and the influence in clotting time to 5 FU 5 fluorouracil

Note：The * P ＜ 0.05 compared with model group, * * P ＜ 0.01

The result shows that more normal group of model group clotting time of mice is obviously prolonged (P ＜ 0.01), bonemarrow nucleated cells number is shown It writes and reduces (P ＜ 0.01), illustrate that model group mouse may show blood coagulation disorders, medulla hematopoietic system suppression, with marrow The Clinical symptoms of inhibition is consistent, illustrates modeling success.Join in Chinese herbaceous peony nourishing qi and blood particle, large dosage of group (7.56,15.12g crude drugs/ Kg) clotting time can be shortened to some extent compared with model group, increase bonemarrow nucleated cells number (P ＜ 0.05 or P ＜ 0.01).It carries Show：Ginseng Chinese herbaceous peony nourishing qi and blood particle can improve the bleeding of Acute Meyloid mouse, myelosuppression.

4. conclusion

The present invention join Chinese herbaceous peony nourishing qi and blood particle large dosage can increase 5 FU 5 fluorouracil cause bone marrow suppression model mice WBC, PLT, granulophilocyte, bonemarrow nucleated cells number shorten the clotting time, cause chmice acute bone marrow suppression to have 5 FU 5 fluorouracil pre- Anti- effect.

Bibliography

[1] Qi Chen, Zhang Baili edit herbal medicine efficacies research methodology-alpastic anemia herbal medicine efficacy research method Beijing：People's Health Publisher, 2016,566-570.

[2] Sun Jiyuan, Wang Siwang, Xie Yanhua wait the animal model experiment of alpastic anemia to study [J] China real Test zoology magazine, 2000 (4)：210-212.

[3] Xu Yan, Cheng Guangyu, Chou Zhikai wait to join red particle of enriching blood poor to acetylphenylhydrazine/cyclophosphamide aplastic Influence [J] ginsengs research of blood animal pattern hematopoiesis function and Hemopoietic factor M μ lti-CSF, EPO contents, 2014,26 (4)： 16-19.

[4] Harbin experimental study [D] for slandering cottonrose hibiscus Wei Chinese medicine X prescriptions treatment aplastic anemia in mice is pedagogical big It learns, 2012.

[5] Zhang Fengfeng, Zhao Zongjiang, Zhang Xinxue wait kidney tonifying marrow facilitating green blood methods to cause Acute Meyloid to inhibit benzene and cyclophosphamide Rat marrow hematopoiesis and the influence of immune function [J] China traditional Chinese medicine magazine, 2014,29 (7)：2326-2330.

[6] Zhou Aixiang, Wang Tianen, Yang Jingmin wait experimental study [J] of Effect of Yishen Shengxue Tablet in Treating Aplastic Anemia Chinese combination of Chinese tradiational and Western medicine magazine, 1999 (3)：43-46.

[7] Cao Rui, Zhang Bo, Wang Guangjian wait mitomycins and 5 FU 5 fluorouracil to induce mouse platelets reduction, leucocyte Reduce the northwests foundation [J] the pharmaceutical journal of animal model, 2011,26 (06)：432-433.

[8] Wang Ronghua, Chen Xinyi, grace family wait the red green blood side of dragons to lead to rat model peripheral hemogram to 5 FU 5 fluorouracil And influence [J] the China traditional Chinese medicine magazine of bone marrow cell quantity, 2014,29 (06)：2023-2026.

[9] in great waves, Wang Lingjie, Liu Tianliang wait rightings Hematogenesis capsule to cause the protective effect that mouse bone marrow cells inhibit to chemotherapy The West China [J] pharmaceutical journal, 2017,32 (6)：575-578.

Three, present invention ginseng Chinese herbaceous peony nourishing qi and blood particle is compared with the prior art experiment

Experiment purpose：By to the present invention ginseng Chinese herbaceous peony nourishing qi and blood particle and prior art CN106214828A enrich blood it is foster Blood, the pharmacological experiment study for restoring marrow function, promoting the effects that blood platelet, by present invention ginseng Chinese herbaceous peony nourishing qi and blood particle and existing Technology CN106214828A is compared, and the power of its pharmacological action is observed.

Experiment drug：

1, a groups：For the prior art, according to Publication No. disclosed on December 14th, 2016 Chinese patent publication CN106214828A, embodiment 1 in the specific implementation mode of entitled " a kind of the Chinese medicine of passage, nourishing blood and preparation method thereof " Method is made.

2, present invention ginseng Chinese herbaceous peony nourishing qi and blood particle：It is made according to the prescription and preparation method of description of the invention embodiment 1.

Test method：The present invention joins Chinese herbaceous peony nourishing qi and blood particle group and a groups are small to caused by cyclophosphamide alpastic anemia The influence of mouse blood picture；

Influence to deficiency of blood mouse blood；Influence to caused by cyclophosphamide mouse bone marrow cells karyocyte reduction；To ring phosphorus The influence of amide induced mice decrease of platelet.

(1) to the influence of caused by cyclophosphamide Induced Aplastic Anemia Mice blood picture

Experiment material

1, animal：Kunming mouse, male and female have concurrently, 18~22g of weight.

2, drug：A groups and present invention ginseng Chinese herbaceous peony nourishing qi and blood particle three groups of large, medium and small doses；Drug is before experiment with steaming Distilled water configures, gastric infusion.

Experimental method

Kunming mouse 60, half male and half female, 18~22g of weight are randomly divided into 6 groups, every group 10.Control group and model The physiological saline of group gavage same volume；The large, medium and small dosage group difference gastric infusion 15.12 of present invention ginseng Chinese herbaceous peony nourishing qi and blood particle, 7.56,1.26g crude drugs/kg；A group gastric infusion 15.12g crude drugs/kg.Successive administration 10d, one time a day.In addition to control group, in Next day, only (50mg/kg) was subcutaneously injected with cyclophosphamide 0.2ml/, 1 time every other day, continuous 4 times.Mouse is put into cylinder simultaneously It is interior, with toluene 30mg/L Inhalations, one time a day × 2h, total 8d.In 10d wholes mouse blood red egg is surveyed from tail vein blood In vain, leucocyte, blood platelet.Experimental result：It is shown in Table 1

Influence of the table 1 to caused by cyclophosphamide Induced Aplastic Anemia Mice blood picture

The * * P ＜ 0.01 compared with model group；With a group ratio △ P ＜ 0.05.

The result shows that：The present invention joins Chinese herbaceous peony nourishing qi and blood particle group and blood hemoglobin, leucocyte, the blood of a group mouse are small Plate is increased significantly, and has pole significant difference (P ＜ 0.01) compared with model group；The present invention joins big dose of Chinese herbaceous peony nourishing qi and blood particle Amount group more significant difference (P ＜ 0.05) compared with a groups.As it can be seen that the present invention, which joins Chinese herbaceous peony nourishing qi and blood particle ratio a groups, reduces ring phosphorus Amide and toluene are strong to the toxicity of marrow, the effect of stimulation myeloid element.

(2) to the influence of deficiency of blood mouse blood

Experiment material

1, animal：Kunming mouse, half male and half female, 18~22g of weight.

Experimental method

Kunming mouse 60, half male and half female, 18~22g of weight are randomly divided into 6 groups, every group 10.In addition to control group, Remaining 5 groups of mouse is distinguished into tail portion bloodletting 0.5m1, so that animal is lost blood, causes Lost blood anemia model, for 24 hours rear gastric infusion.It is right According to the physiological saline of group and model group gavage same volume；The present invention joins the large, medium and small dosage group of Chinese herbaceous peony nourishing qi and blood particle and distinguishes gavage Administration 15.12,7.56,1.26g crude drugs/kg；A group gastric infusion 15.12g crude drugs/kg.Successive administration 7d, one time a day, last After 2h is administered, tail portion takes the value of blood survey hemoglobin and red blood cell.Experimental result：It is shown in Table 2

Influence of the table 2 to deficiency of blood mouse blood

The result shows that：The present invention joins Chinese herbaceous peony nourishing qi and blood particle group and a groups are remarkably improved mouse hemoglobin and red blood cell Level, have pole significant difference (P ＜ 0.01) compared with model group；The present invention joins Chinese herbaceous peony nourishing qi and blood particle large dosage group and a Group compares more significant difference (P ＜ 0.05).As it can be seen that present invention ginseng Chinese herbaceous peony nourishing qi and blood particle group ratio a group blood tonification effects are strong.

(3) to the influence of caused by cyclophosphamide mouse bone marrow cells karyocyte reduction

Experiment material

1, animal：Kunming mouse, half male and half female, 18~22g of weight.

Experimental method

Kunming mouse 60, half male and half female, 18~22g of weight are randomly divided into 6 groups, every group 10.In addition to control group, Each group mouse subcutaneous injection cyclophosphamide 70mg/kg, totally 2 times.The physiological saline of control group and model group gavage same volume；This hair The bright large, medium and small dosage group of ginseng Chinese herbaceous peony nourishing qi and blood particle distinguishes gastric infusion 15.12,7.56,1.26g crude drugs/kg；A group gavages are given Medicine 15.12g crude drugs/kg.Successive administration 7d puts to death animal in administration 8d and side femur, meter marrow is taken to have core thin one time a day Born of the same parents' number.Experimental result：It is shown in Table 3

Influence of the table 3 to caused by cyclophosphamide mouse bone marrow cells karyocyte reduction

The * * P ＜ 0.01 compared with model；With a group ratio △ P ＜ 0.05.

The result shows that：The present invention joins Chinese herbaceous peony nourishing qi and blood particle group and a groups to caused by cyclophosphamide mouse bone marrow cells karyocyte Reduction obviously increase effect, have pole significant difference (P ＜ 0.01) compared with model group；The present invention joins Chinese herbaceous peony nourishing qi and blood particle The large dosage of group of more significant difference (P ＜ 0.05) compared with a groups.Promote as it can be seen that the present invention joins Chinese herbaceous peony nourishing qi and blood particle group ratio a groups The effect restored into impaired hemopoietic function of bone marrow is strong.

(4) to the influence of caused by cyclophosphamide mouse platelets reduction

Experiment material

1, animal：Kunming mouse, half male and half female, 18~22g of weight.

Experimental method

Kunming mouse 60, half male and half female, 18~22g of weight are randomly divided into 6 groups, every group 10.Control group and model The distilled water of group gavage same volume；The large, medium and small dosage group difference gastric infusion 15.12 of present invention ginseng Chinese herbaceous peony nourishing qi and blood particle, 7.56,1.26g crude drugs/kg；A group gastric infusion 15.12g crude drugs/kg.Successive administration 15d, one time a day, in addition to control group, 5 groups of mouse remaining in 8d, 10d and 12d intraperitoneal injection of cyclophosphamide 100g/kg, mouse platelets is caused to reduce, in the ends 15d After secondary administration 2h, eye socket blood sampling calculates platelet count.Experimental result：It is shown in Table 4

Influence of the table 4 to caused by cyclophosphamide mouse platelets reduction

The * * P ＜ 0.01 compared with model；With a group ratio △ P ＜ 0.05.

The result shows that：The present invention joins caused by Chinese herbaceous peony nourishing qi and blood particle group and a the groups substantially reduced injection cyclophosphamide of energy Decrease of platelet, have pole significant difference (P ＜ 0.01) compared with model group；The present invention joins Chinese herbaceous peony nourishing qi and blood particle large dosage Group more significant difference (P ＜ 0.05) compared with a groups.As it can be seen that present invention ginseng Chinese herbaceous peony nourishing qi and blood particle group ratio a groups raising blood is small The effect of plate is strong.

Experimental result：The present invention joins Chinese herbaceous peony nourishing qi and blood particle group and a groups are substantially reduced cyclophosphamide and toluene to marrow Toxicity increases mouse blood hemoglobin, leucocyte, blood platelet；It is remarkably improved the level of mouse hemoglobin and red blood cell； The reduction of caused by cyclophosphamide mouse bone marrow cells karyocyte is had significantly increasing action；Caused by cyclophosphamide mouse platelets are subtracted The effect of rare raising.

Conclusion：The present invention joins the blood-supplementing blood-nourishing of Chinese herbaceous peony nourishing qi and blood particle group ratio a groups, restores marrow function, promotes blood platelet Equal pharmacological actions are strong.Therefore, clinically present invention ginseng Chinese herbaceous peony nourishing qi and blood particle group ratio a groups treat alpastic anemia, marrow Hyperplasia exception syndrome, thrombopenia and chemicotherapy cause the effect of cytopenia strong.

Description of the drawings

The present invention joins No. 10 mouse main organs diseases of Chinese herbaceous peony No. 3 mouse of nourishing qi and blood particle acute toxicity test control group and administration group Neo-Confucianism checks that photo (HE is dyed, HE × 100) is shown in Fig. 1-Figure 12；

Fig. 1 is No. 3 mouse heart pathological examination photos of control group；

Fig. 2 is No. 10 mouse heart pathological examination photos of administration group；

Fig. 3 is No. 3 mouse liver pathological examination photos of control group；

Fig. 4 is No. 10 mouse liver pathological examination photos of administration group；

Fig. 5 is No. 3 mouse spleen pathological examination photos of control group；

Fig. 6 is No. 10 mouse spleen pathological examination photos of administration group；

Fig. 7 is No. 3 mouse lung pathological examination photos of control group；

Fig. 8 is No. 10 mouse lung pathological examination photos of administration group；

Fig. 9 is No. 3 mouse kidney pathological examination photos of control group；

Figure 10 is No. 10 mouse kidney pathological examination photos of administration group；

Figure 11 is No. 3 Mouse Stomach pathological examination photos of control group；

Figure 12 is No. 10 Mouse Stomach pathological examination photos of administration group.

The specific embodiment of the invention：

The present invention is further explained in the light of specific embodiments, but not as limitation of the present invention.

Embodiment 1：

The present invention is made of the bulk pharmaceutical chemicals of following weight proportion：

Preparation method is as follows：

Embodiment 2：

Preparation method is as follows：

Embodiment 3：

Preparation method is as follows：

Embodiment 4：

Preparation method is as follows：

Embodiment 5：

Preparation method is as follows：

Embodiment 6：

Preparation method is as follows：

Embodiment 7：

Preparation method is as follows：

Embodiment 8：

Preparation method is as follows：

Claims

1. a seed ginseng Chinese herbaceous peony nourishing qi and blood particle Chinese medicine composition, which is characterized in that it is by the bulk pharmaceutical chemicals system of following weight proportion At：

2. seed ginseng Chinese herbaceous peony nourishing qi and blood particle Chinese medicine composition according to claim 1, which is characterized in that it is by following The bulk pharmaceutical chemicals of preferred weight proportioning are made：

3. the preparation method of seed ginseng Chinese herbaceous peony nourishing qi and blood particle Chinese medicine composition according to claim 1 or 2, feature exist In including the following steps：Above 12 taste adds water to cook secondary, 1.5 hours first times, and 8 times of water is added to measure, second 1 hour, 6 times of amounts of water, collecting decoction are added to filter, filtrate measures the clear cream that relative density is 1.20-1.25 when being condensed into 60 DEG C, 70 DEG C subtract Pressure is dried, crushed into fine powder；Take extract powder, dextrin, cane sugar powder by 1:0.5:Particle is made with 80% ethyl alcohol in 1 ratio mixing, Dry, 1000g is made in whole grain, dispenses to get granule.

4. seed ginseng Chinese herbaceous peony nourishing qi and blood particle Chinese medicine composition according to claim 1 or 2, which is characterized in that main to use Cytopenia, card are caused in treatment alpastic anemia, myelodysplastic syndrome, thrombopenia and chemicotherapy See qi-blood deficiency, the insufficiency of the kidney yang.