CN1084712A - Glossy ganoderma nurition health care liquid and preparation method thereof - Google Patents
Glossy ganoderma nurition health care liquid and preparation method thereof Download PDFInfo
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- CN1084712A CN1084712A CN92111030A CN92111030A CN1084712A CN 1084712 A CN1084712 A CN 1084712A CN 92111030 A CN92111030 A CN 92111030A CN 92111030 A CN92111030 A CN 92111030A CN 1084712 A CN1084712 A CN 1084712A
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Abstract
The invention belongs to the allotment of non-alcoholic beverage, it is the cultivation that comprises ganoderma lucidum mycelium, the production and processing of ganoderma lucidum mycelium, fructification extract, and the preparation of nourishing health care liquid, ganoderma lucidum mycelium are to gathering, the transformation of germanium, adopt submerged fermentation method production to be rich in the ganoderma lucidum mycelium of organic germanium, and mycelium and fructification are carried out that comparatively simple water is carried, alcohol extracting concentrates and processing such as allotment is handled.Need not add valuable medicinals such as genseng, the fleece-flower root, guarantee organic germanium in the oral liquid, polysaccharide and various amino acid whose content effectively, be a kind of comparatively desirable glossy ganoderma nurition health care liquid.
Description
The invention belongs to non-alcoholic beverage, is the cultivation that comprises ganoderma lucidum mycelium, the preparation method of the production and processing of ganoderma lucidum mycelium, fructification extract and the contents such as allotment of nourishing health care liquid.
At present, with the glossy ganoderma is raw material, the edible medicine or the health food that contain the glossy ganoderma effective ingredient, beverage has polytype, (magazine is brewageed by China as " glossy ganoderma health beverage " wherein to make the relevant report of oral liquid formulation, 86 years the 4th phases), " a kind of production technology of glossy ganoderma essence " (the 6th 15 phases of volume of Chinese invention patent communique), prepare these two kinds of selected raw materials of oral liquid and be the glossy ganoderma of artificial culture, active ingredient is wherein extracted from the fructification of glossy ganoderma mostly, adopt low temperature reflux water to extract and Different concentrations of alcohol reflux extraction process combined flow preparation lucidum concentrating liquid, and palpus and genseng, the allotment of valuable drugs such as the fleece-flower root.Because is the glossy ganoderma that primary raw material is cultivated with conventional method with cotton seed hull, wood chip, does not contain organic germanium in its fructification substantially, though contain organic germanium in the natural glossy ganoderma, because of its limited amount, can not become the raw material of industrialized mass production.Cause and comprise this two kinds of oral agents, with the artificial culture glossy ganoderma is the existing various medicines of raw material or health food, beverage, the difficulty that exists industrialized mass production is bigger, the content of organic germanium and other nutritional labelings is low in the finished product sticks with paste the embedding operation with palpus increase ring, to alleviate the deficiencies such as bitter taste that caused because of the bitter taste glycocide in the ganoderma lucidum fruitbody.
The object of the present invention is to provide a kind of glossy ganoderma with artificial culture is the glossy ganoderma nurition health care liquid of primary raw material, this health care liquid utilizes gathering, the transformation of ganoderma lucidum mycelium to germanium, take submerged fermentation method production to be rich in the ganoderma lucidum mycelium of organic germanium, and mycelium, fructification carried out that comparatively simple water is carried, alcohol extracting concentrates and processing and preparing such as allotment, to overcome the existing in prior technology deficiency.
Overall technical architecture of the present invention is:
Choose the solid medium and the germanic fluid nutrient medium of ganoderma strain capable and adaptation Ganoderma lucidum mycelium bulk-growth, be rich in the ganoderma lucidum mycelium of germanium by tank fermentation method production, after the processing processing, the material allotments such as stoste, honey, agar, Sodium Benzoate that with its stoste serve as main and ganoderma lucidum fruitbody are made, the former liquid hold-up that it is characterized in that fructification in this health care liquid is 8-15%, honey content is 8-15%, 2% agar content is 8-15%, Sodium Benzoate content is 0.17-0.2%, and surplus is the stoste of ganoderma lucidum mycelium;
The cultivation of ganoderma lucidum mycelium and the preparation method of extract thereof are as follows:
A, the breezing and producing method of ganoderma lucidum mycelium: at first the potato in order to 15%-25% cooks liquid in aseptic still, the carrot of 10%-20% cooks liquid, it is main preparation inclined-plane solid medium that sawdust cooks liquid, during inoculation, selected at least a ganoderma strain capable, inoculation condition is: room temperature, unglazed, using shaking table to cultivate then enlarges, after 3-5 days, the ganoderma lucidum mycelium that grows is sent into first class seed pot and fermentation tank at least successively, send fluid nutrient medium simultaneously to and feed aseptic compressed air, carry out the deep ventilation pure culture, time is 1-2 days in every grade of seeding tank, fermentation time is 3-5 days, fluid nutrient medium is mainly to contain corn flour, sucrose solution, and the germanium liquid of interpolation 40-100PPm;
The preparation of B, ganoderma lucidum mycelium extract: after fermentation is finished, it is stand-by to send into hold-up tank, during preparation after the plate filter press filtration, separation of mycelial and zymotic fluid, mycelium is carried out poach, filtration, and to being mixed and made into the Ganoderma lucidum mycelium extract with the zymotic fluid that has concentrated again after the filtrate concentration;
The preparation of ganoderma lucidum fruitbody extract:
A, infuse: after the fructification of getting glossy ganoderma is pulverized, under 25-30 ℃ of temperature with 80% alcohol immersion 2-4 days.
B, pure immersion liquid are extracted: gained filtrate behind the pure immersion liquid press filtration is carried out decompression distillation, is the alcohol extracting concentrate of ganoderma lucidum fruitbody behind the recovery ethanol;
C, poach extract: will filter the gained ganoderma lucidum fruitbody and immerse deionized water, boil after filtering in 20-30 minute the poach concentrate, poach extraction concentrate process is carried out once at least;
Mix above-mentioned two kinds of alcohol extracting concentrates and poach concentrate, poach 20-30 minute is the extract of ganoderma lucidum fruitbody again;
Extract to ganoderma lucidum mycelium, fructification concentrates, and makes its concentration be 8-15g/10
2Ml is as the preparation ganoderma lucidum mycelium stoste used of nutrient solution and the stoste of ganoderma lucidum fruitbody.
Its solid medium and liquid are cultivated base:
The solid medium additive wherein of inoculation usefulness also has glucose, potassium dihydrogen phosphate, magnesium sulfate, VB
1, agar, or select a kind of in conventional PDA solid medium, the comprehensive potato culture for use; Additive in the fluid nutrient medium also has ammonium sulfate, potassium dihydrogen phosphate, magnesium sulfate, calcium carbonate, VB
1, or select for use based on sesame meal, sucrose, and add the fluid nutrient medium of potassium dihydrogen phosphate, magnesium sulfate, ammonium sulfate, each material of calcium carbonate, and in the deep ventilation pure culture process two-stage seeding tank being set, its volume increases successively.
Selected ganoderma strain capable is:
Selected ganoderma strain capable is 1#:GANODERMA, LUCIDUM, 2#: " Taishan glossy ganoderma ", 3#: " Japanese precocious glossy ganoderma ", a kind of among the 4#:G.SINENSE.
The water of ganoderma lucidum fruitbody is carried method for concentration is:
It is twice that the poach of ganoderma lucidum fruitbody stoste extracts concentration process, being about to water cooking liquid gained ganoderma lucidum fruitbody clinker for the first time, to add the poach concentrate that deionized water carries out after poach filters again be secondary poach concentrate, will twice poach concentrate and the alcohol extracting concentrate carry out poach again after mixing and concentrate the extract of producing fructification.
Accompanying drawing of the present invention is the cultivation of ganoderma lucidum mycelium and preparation technology's flow chart of nourishing health care liquid.
In conjunction with the accompanying drawings embodiments of the invention are described further.
1, the breezing and producing method of ganoderma lucidum mycelium:
1. the apolegamy of bacterial strain, cultivation base: choose 1# or 4# bacterial strain.Solid medium is to cook liquid with 20% potato, and 15% carrot cooks liquid and sawdust, and to cook liquid be main a small amount of glucose, agar and micro-potassium dihydrogen phosphate, magnesium sulfate, the VB of adding
1Formulated, the relative proportioning of each component is 1: 0.6: 0.4: 0.04: 0.04: 0.006: 0.003: 0.001.The relative scale of fluid nutrient medium each component corn flour, sucrose, ammonium sulfate, potassium dihydrogen phosphate, magnesium sulfate, calcium carbonate is 1: 1: 0.13: 0.08: 0.04: 0.1, and add trace V B
1Germanium liquid with 45PPm.
2. the breezing and producing method of ganoderma lucidum mycelium: be the pure cultivation of deep ventilation.Concrete technological process as shown in drawings, from " slant strains " to " storage tank " each manufacturing procedure before.Because of the glossy ganoderma bacterium does not produce spore, grow up by the mycelia division, so fermentation time is 4 days, inoculum concentration is 5-10%, and it is 29 ± 1 ℃ that inoculation and shaking table are cultivated temperature, adopts the two-stage seeding tank in addition in suitability for industrialized production, and the volume of each seeding tank is respectively 0.1m
3And 1m
3The time of storing in each seeding tank is 36 hours.
2, the extraction of mycelium nutritional labeling and active ingredient:
Behind the complete fermentation about 6 days, organic germanium content reaches as high as 32.5 μ g/g in the mycelium, and it comprises organic germanium sesquioxide, the organic germanium sesquisulfide, and uracil germanium sesquichloride, organic germanium acid anhydrides etc. are low molecule germanide.Also have the product that germanium ethide sesquichloride and germanium combines with amino acid, the chromium complex of germanium, the macromolecule germanides such as chelate of germanium.The extraction preparation method of its stoste is aforementioned.
3, the extraction of ganoderma lucidum fruitbody nutrition and active ingredient:
Get red sesame and purple sesame and pulverize the back and adopt alcohol extracting and water to put forward the method that combines to prepare ganoderma lucidum fruitbody stoste, concrete technological process as described above.
4, the allotment of glossy ganoderma nurition health care liquid:
Get the mycelium stoste 70% for preparing, add fructification stoste 10%, the agar 10% of honey 10%, 2%, Sodium Benzoate 0.2% are deployed into the muddy shape liquid of light brown, the little hardship of mouthfeel, and sweet thick, wherein soluble solid is 17%, and proportion is about 1.1, and pH value is about 5.
The substantive distinguishing features that the present invention has and obtained remarkable technological progress:
1, on the basis of choosing ganoderma strain capable and corresponding solid, fluid nutrient medium, its fast growth of ganoderma lucidum mycelium that utilizes deep liquid to cultivate, the mycelium quality is higher, no Only the nutrition in its extract and effective component content such as each seed amino acid are not less than ganoderma lucidum fruitbody, and can realize the industrialized mass production of ganoderma lucidum mycelium.
2, owing in the deep liquid incubation, for gathering, the conversion characteristic of ganoderma lucidum mycelium to germanium, add a certain amount of germanium in the liquid medium within, the content that makes organic germanium in the ganoderma lucidum mycelium is 4-6 times of ginseng, and its polyoses content is also higher. Therefore select artificial ganoderma that the present invention cultivates production must not add the valuable medicinals such as ginseng, the fleece-flower root and both guaranteed the organic germanium in the oral liquid, polysaccharide and various amino acid whose content for primary raw material prepares oral liquid, also simplify preparation section, reduced cost.
3, ganoderma lucidum mycelium, fructification are carried out the PROCESS FOR TREATMENT such as alcohol extracting, water extraction and concentration, preparation, advanced technology is reasonable, by analysis, chemical examination shows and can basically contained various nutritions, effective ingredient in mycelium, the fructification be extracted fully, particularly when allotment, add a certain amount of fructification stoste, not only removed the operation with cyclodextrin embedding glossy ganoderma bitter taste glycocide, and control and kept bitter taste as glossy ganoderma class oral liquid uniqueness.
Claims (4)
1, glossy ganoderma nurition health care liquid and preparation method thereof is solid medium and the germanic fluid nutrient medium of choosing ganoderma strain capable and adapting to the Ganoderma lucidum mycelium bulk-growth, be rich in the ganoderma lucidum mycelium of germanium by tank fermentation method production, after the processing processing, the material allotments such as stoste, honey, agar, Sodium Benzoate that with its stoste serve as main and ganoderma lucidum fruitbody are made, the former liquid hold-up that it is characterized in that fructification in this health care liquid is 8-15%, honey content is 8-15%, 2% agar content is 8-15%, Sodium Benzoate content is 0.17-0.2%, and surplus is the stoste of ganoderma lucidum mycelium;
The cultivation of ganoderma lucidum mycelium and the preparation method of extract thereof are as follows:
A, the breezing and producing method of ganoderma lucidum mycelium: at first the potato in order to 15%-25% cooks liquid in aseptic still, the carrot of 10%-20% cooks liquid, it is main preparation inclined-plane solid medium that sawdust cooks liquid, during inoculation, selected at least a ganoderma strain capable, inoculation condition is: room temperature, unglazed, using shaking table to cultivate then enlarges, after 3-5 days, the ganoderma lucidum mycelium that grows is sent into first class seed pot and fermentation tank at least successively, send fluid nutrient medium simultaneously to and feed aseptic compressed air, carry out the deep ventilation pure culture, time is 1-2 days in every grade of seeding tank, fermentation time is 3-5 days, fluid nutrient medium is mainly to contain corn flour, sucrose solution, and the germanium liquid of interpolation 40-100PPm;
The preparation of B, ganoderma lucidum mycelium extract: after fermentation is finished, it is stand-by to send into hold-up tank, during preparation after the plate filter press filtration, separation of mycelial and zymotic fluid, mycelium is carried out poach, filtration, and to being mixed and made into the Ganoderma lucidum mycelium extract with the zymotic fluid that has concentrated again after the filtrate concentration;
The preparation of ganoderma lucidum fruitbody extract:
A, infuse: after the fructification of getting glossy ganoderma is pulverized, under 25-30 ℃ of temperature with 80% alcohol immersion 2-4 days.
B, pure immersion liquid are extracted: gained filtrate behind the pure immersion liquid press filtration is carried out decompression distillation, is the alcohol extracting concentrate of ganoderma lucidum fruitbody behind the recovery ethanol;
C, poach extract: will filter the gained ganoderma lucidum fruitbody and immerse deionized water, boil after filtering in 20-30 minute the poach concentrate, poach extraction concentrate process is carried out once at least;
Mix above-mentioned two kinds of alcohol extracting concentrates and poach concentrate, poach 20-30 minute is the extract of ganoderma lucidum fruitbody again;
Extract to ganoderma lucidum mycelium, fructification concentrates, and makes its concentration be 8-158/10
2Ml is as the preparation ganoderma lucidum mycelium stoste used of nutrient solution and the stoste of ganoderma lucidum fruitbody.
2, health care liquid according to claim 1 and preparation method thereof is characterized in that the solid medium additive wherein of inoculating usefulness also has glucose, potassium dihydrogen phosphate, magnesium sulfate, VB
1Agar, or select a kind of in conventional PDA solid medium, the comprehensive potato culture for use; Additive in the fluid nutrient medium also has ammonium sulfate, potassium dihydrogen phosphate, magnesium sulfate, calcium carbonate, VB
1, or select for use based on sesame meal, sucrose, and add the fluid nutrient medium of potassium dihydrogen phosphate, magnesium sulfate, ammonium sulfate, each material of calcium carbonate, and in the deep ventilation pure culture process two-stage seeding tank being set, its volume increases successively.
3, health care liquid according to claim 1 and 2 and preparation method thereof is characterized in that selected ganoderma strain capable is 1#:GANODERMA, LUCIDUM, 2#: " Taishan glossy ganoderma ", 3#: " Japanese precocious glossy ganoderma ", a kind of among the 4#:G.SINENSE.
4, health care liquid according to claim 2 and preparation method thereof, it is characterized in that the poach extraction concentration process to ganoderma lucidum fruitbody stoste is twice, being about to water cooking liquid gained ganoderma lucidum fruitbody clinker for the first time, to add the poach concentrate that deionized water carries out after poach filters again be secondary poach concentrate, concentrate carrying out poach again after twice poach concentrate and the mixing of alcohol extracting concentrate, produce the extract of fructification.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN92111030A CN1084712A (en) | 1992-09-28 | 1992-09-28 | Glossy ganoderma nurition health care liquid and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN92111030A CN1084712A (en) | 1992-09-28 | 1992-09-28 | Glossy ganoderma nurition health care liquid and preparation method thereof |
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| CN1084712A true CN1084712A (en) | 1994-04-06 |
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| CN92111030A Pending CN1084712A (en) | 1992-09-28 | 1992-09-28 | Glossy ganoderma nurition health care liquid and preparation method thereof |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1060031C (en) * | 1994-05-28 | 2001-01-03 | 徐士杰 | Lucid ganoderma syrup and its preparation |
| CN1060632C (en) * | 1996-08-28 | 2001-01-17 | 海南保丽康生物科技食品有限责任公司 | Chinese glossy ganoderma health beverage |
| CN102578665A (en) * | 2012-02-23 | 2012-07-18 | 武斌 | Preparation method of Ganoderma beverage |
| CN102771776A (en) * | 2012-07-20 | 2012-11-14 | 黄晓青 | Ganoderma sinense health-care product containing traditional Chinese medicine extractives and preparation method of ganoderma sinense health-care product containing traditional Chinese medicine extractives |
| CN101558720B (en) * | 2009-04-29 | 2013-04-17 | 大连三禾生物有限公司 | Method for producing germanium-enriched glossy ganoderma mycelium |
| CN103525684A (en) * | 2013-10-28 | 2014-01-22 | 山西省农业科学院食用菌研究所 | Method for preparing ganoderma lucidum vinegar by utilization of ganoderma lucidum submerged fermentation liquid |
| CN104957514A (en) * | 2015-07-09 | 2015-10-07 | 淮阴师范学院 | Method for preparing lucid ganoderma rice meal by utilizing ganoderma lucidum mycelia to ferment rice |
| CN106072632A (en) * | 2016-06-15 | 2016-11-09 | 钱卫文 | A kind of Ganoderma extract and its preparation method and application |
| CN108157714A (en) * | 2017-12-26 | 2018-06-15 | 广西巴马中脉寿乡矿泉水有限公司 | A kind of ganoderma lucidum mineral water |
| CN109198602A (en) * | 2018-07-09 | 2019-01-15 | 开平健之源保健食品有限公司 | A kind of composite glossy ganoderma polyoses oral liquid and preparation method thereof |
| CN115644338A (en) * | 2022-11-03 | 2023-01-31 | 湖南迪为农业科技有限公司 | Lucid ganoderma health-preserving water beverage |
-
1992
- 1992-09-28 CN CN92111030A patent/CN1084712A/en active Pending
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1060031C (en) * | 1994-05-28 | 2001-01-03 | 徐士杰 | Lucid ganoderma syrup and its preparation |
| CN1060632C (en) * | 1996-08-28 | 2001-01-17 | 海南保丽康生物科技食品有限责任公司 | Chinese glossy ganoderma health beverage |
| CN101558720B (en) * | 2009-04-29 | 2013-04-17 | 大连三禾生物有限公司 | Method for producing germanium-enriched glossy ganoderma mycelium |
| CN102578665A (en) * | 2012-02-23 | 2012-07-18 | 武斌 | Preparation method of Ganoderma beverage |
| CN102771776A (en) * | 2012-07-20 | 2012-11-14 | 黄晓青 | Ganoderma sinense health-care product containing traditional Chinese medicine extractives and preparation method of ganoderma sinense health-care product containing traditional Chinese medicine extractives |
| CN103525684B (en) * | 2013-10-28 | 2015-08-19 | 山西省农业科学院食用菌研究所 | Deep glossy ganoderma fermenting liquid prepares the method for Ganoderma vinegar |
| CN103525684A (en) * | 2013-10-28 | 2014-01-22 | 山西省农业科学院食用菌研究所 | Method for preparing ganoderma lucidum vinegar by utilization of ganoderma lucidum submerged fermentation liquid |
| CN104957514A (en) * | 2015-07-09 | 2015-10-07 | 淮阴师范学院 | Method for preparing lucid ganoderma rice meal by utilizing ganoderma lucidum mycelia to ferment rice |
| CN106072632A (en) * | 2016-06-15 | 2016-11-09 | 钱卫文 | A kind of Ganoderma extract and its preparation method and application |
| CN106072632B (en) * | 2016-06-15 | 2019-12-27 | 钱卫文 | Ganoderma lucidum extract and preparation method and application thereof |
| CN108157714A (en) * | 2017-12-26 | 2018-06-15 | 广西巴马中脉寿乡矿泉水有限公司 | A kind of ganoderma lucidum mineral water |
| CN109198602A (en) * | 2018-07-09 | 2019-01-15 | 开平健之源保健食品有限公司 | A kind of composite glossy ganoderma polyoses oral liquid and preparation method thereof |
| CN109198602B (en) * | 2018-07-09 | 2022-04-05 | 开平健之源保健食品有限公司 | Compound ganoderma lucidum polysaccharide oral liquid and preparation method thereof |
| CN115644338A (en) * | 2022-11-03 | 2023-01-31 | 湖南迪为农业科技有限公司 | Lucid ganoderma health-preserving water beverage |
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