CN108464415A - A kind of beverage containing fish scale collagen - Google Patents
A kind of beverage containing fish scale collagen Download PDFInfo
- Publication number
- CN108464415A CN108464415A CN201810173996.4A CN201810173996A CN108464415A CN 108464415 A CN108464415 A CN 108464415A CN 201810173996 A CN201810173996 A CN 201810173996A CN 108464415 A CN108464415 A CN 108464415A
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- CN
- China
- Prior art keywords
- fish scale
- collagen
- water
- beverage containing
- added
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
Abstract
It is composed of the following components the present invention relates to a kind of beverage containing fish scale collagen:Fish scale collagen 2 6%, white granulated sugar 2 4%, citric acid 1 3%, inspissated juice 10 20%, honey 2 4%, surplus are water.Contain fish scale collagen in beverage prepared by the present invention, the collagen of needed by human body can be supplemented in the case of moisturizing, collagen of the invention comes from fish scale, of low cost.
Description
Technical field
The present invention relates to the preparing technical field of biological collagen, more particularly to a kind of drink containing fish scale collagen
Material.
Background technology
Collagen is one of main component of human tissue structure, is the spirality fiber shape egg twisted by three peptide chains
White matter is a kind of glycoprotein, and molecule is containing sugar and a large amount of glycine, proline, hydroxyprolines etc., and there are all multicellular animals
A kind of most protein of interior and people's in-vivo content, accounts for about a quarter of total protein content, is present in several in human body
Institute in a organized way in, be a kind of extracellular protein, there is height tensile energy, to animal and human skin, blood vessel, bone,
Muscles and bones, the formation of cartilage are particularly significant, have height tensile energy, are the important substances of connective tissue, are to determine connective tissue
The principal element of toughness.The effect that Hydrolyzed Collagen is conducive to health is taken, embodiment is obtained on humans and animals body.
Fish scale is fish body and the extraneous tissue contacted, is the deformations of fish skin corium, usually account for fish body weight 1%~
5%, play a part of that fish body is protected to escape injury.Usual fish scale can be divided into three kinds:(1) the ichthyomorphic wooden placoid scale of the plate gill;(2) tiltedly
The hard shell that square, edge are connected;(3) most common corneoscute, in bony fish at most.Fish scale is mainly by protein, lecithin, hydroxyl
Base apatite and guanine composition, wherein organic matter account for 41% one 55%, calcium salt 38%~46%.Protein accounts for gross weight
70%, predominantly collagen and fish scale keratin.Fish scale collagen is mainly type i collagen.I-type collagen is three strands super
All rotation forms left hand helix structure to the left for helical structure, i.e. three polypeptide chains every, this three peptide chains are mutually tied with hydrogen bond again
Conjunction forms right-handed superhelix structure.This structure is highly stable, collagen general not soluble in water and neutral salt solution, diluted acid, diluted alkaline
And common organic solvents, it is dissolved in strong acid and highly basic.
Collagen can supplement nutrition needed for skin, so that Collage Activitv in skin is enhanced, have skin care, delay to decline
Always, beauty, wrinkle-chasing, hair care and other effects.Livestock and poultry source animal tissue is always that people obtain natural collagen protein and its collagen peptide
Main path.Fish body waste etc. can be used as raw material production collagen, not only improve the quality of collagen, but also expand
The source of collagen raw material, turns waste into wealth, and economizes on resources.
Invention content
For problems of the prior art, the object of the present invention is to provide a kind of preparation sides of fish scale collagen
Method.
In order to solve the above-mentioned technical problem, the present invention is achieved by the following scheme:
A kind of beverage containing fish scale collagen, it is composed of the following components:
Fish scale collagen 2-6%, white granulated sugar 2-4%, citric acid 1-3%, inspissated juice 10-20%, honey 2-4%,
Surplus is water.
A kind of beverage containing fish scale collagen, it is composed of the following components:
Fish scale collagen 4%, white granulated sugar 3%, citric acid 2%, inspissated juice 15%, honey 3%, surplus are water.
On the basis of said program, the fish scale collagen is prepared by following methods:
Step 1, fish scale is put into sink, the warm water of 10 times of fish scale weight is added, 40 DEG C of water temperature is impregnated 1 hour so that
Fish scale rehydration;
Step 2, treated fish scale is added to the 0.5% salt acid soak 2 hours of 10 times of fish scale weight, 40 DEG C of temperature;
Step 3, the solution immersed with fish scale obtained by step 2 is neutralized to neutrality with 0.2% sodium hydroxide;
Step 4, the fish scale after step 3 is washed till neutrality with clear water;
Step 5, the desalted water of 10 times of fish scale weight is added in fish scale, heating is boiled 0.5 hour;
Step 6, it filters, removes fish scale, temperature is cooled to 45 DEG C;
Step 7, the protease of 0.1% mass is added in solution after the cooling period, keeps the temperature 5 hours;Protease is tryptose
The ratio of the composition of enzyme and papain, the two is 1:1.
Step 8, Hydrolyzed Collagen solution made from step 7 is filtered off into removing protein enzyme using diatomaceous earth filter;
Step 9, the Hydrolyzed Collagen solution that protease is removed in step 8 is further filtered using ultrafilter;Ultrafiltration
The ultrafiltration membrane restriction molecule amount of device is 5000 dalton
Step 10, the salt ion in filtrate that step 9 obtains is got rid of using ion exchange resin;
Step 11, it is dried using spray drying process after concentration, the collagen protein powder of finished product.
Advantageous effect:
Contain fish scale collagen in beverage prepared by the present invention, the collagen of needed by human body can be supplemented in the case of moisturizing
Albumen, collagen of the invention comes from fish scale, of low cost;The present invention uses fish scale for raw material, by decalcification, colloidal sol,
Be separated by solid-liquid separation filtering, hydrolysis, collagen is separated by filtration, high molecular weight protein is separated by filtration, desalination, dehydration, drying and other steps, system
Obtain collagen protein powder so that fish scale is turned waste into wealth, and realizes making full use of for resource.The present invention uses joint biological enzyme digestion method will
Macromolecular collagen protein is hydrolyzed into small molecular protein, and biological enzyme hydrolysis is efficient, and safe and nontoxic so that collagen obtained
Albumen can meet food security standard.
Specific implementation mode
Below in conjunction with preferred embodiment, to the specific implementation mode that provides according to the present invention, details are as follows:
Embodiment 1
A kind of preparation method of fish scale collagen, includes the following steps:
Step 1, fish scale is put into sink, the warm water of 10 times of fish scale weight is added, 40 DEG C of water temperature is impregnated 1 hour so that
Fish scale rehydration;
Step 2, treated fish scale is added to the 0.5% salt acid soak 2 hours of 10 times of fish scale weight, 40 DEG C of temperature;
Step 3, the solution immersed with fish scale obtained by step 2 is neutralized to neutrality with 0.2% sodium hydroxide;
Step 4, the fish scale after step 3 is washed till neutrality with clear water;
Step 5, the desalted water of 10 times of fish scale weight is added in fish scale, heating is boiled 0.5 hour;
Step 6, it filters, removes fish scale, temperature is cooled to 45 DEG C;
Step 7, the protease of 0.1% mass is added in solution after the cooling period, keeps the temperature 5 hours;The protease is pancreas
The ratio of the composition of protease and papain, the two is 1:1.
Step 8, Hydrolyzed Collagen solution made from step 7 is filtered off into removing protein enzyme using diatomaceous earth filter;
Step 9, the Hydrolyzed Collagen solution that protease is removed in step 8 is further filtered using ultrafilter;Ultrafiltration
The ultrafiltration membrane restriction molecule amount of device is 5000 dalton
Step 10, the salt ion in filtrate that step 9 obtains is got rid of using ion exchange resin;
Step 11, it is dried using spray drying process after concentration, the collagen protein powder of finished product.
Embodiment 2
A kind of beverage containing fish scale collagen, it is composed of the following components:
Fish scale collagen 2%, white granulated sugar 2%, citric acid 1%, inspissated juice 10%, honey 2%, surplus are water.
Embodiment 3
A kind of beverage containing fish scale collagen, it is composed of the following components:
Fish scale collagen 4%, white granulated sugar 3%, citric acid 2%, inspissated juice 15%, honey 3%, surplus are water.
Embodiment 4
A kind of beverage containing fish scale collagen, it is composed of the following components:
Fish scale collagen 6%, white granulated sugar 4%, citric acid 3%, inspissated juice 20%, honey 4%, surplus are water.
The above described is only a preferred embodiment of the present invention, being not that the invention has other forms of limitations, appoint
What those skilled in the art changed or be modified as possibly also with the technology contents of the disclosure above equivalent variations etc.
Imitate embodiment.But it is every without departing from technical solution of the present invention content, according to the technical essence of the invention to above example institute
Any simple modification, equivalent variations and the remodeling made, still fall within the protection domain of technical solution of the present invention.
Claims (3)
1. a kind of beverage containing fish scale collagen, which is characterized in that composed of the following components:
Fish scale collagen 2-6%, white granulated sugar 2-4%, citric acid 1-3%, inspissated juice 10-20%, honey 2-4%, surplus
For water.
2. a kind of beverage containing fish scale collagen according to claim 1, which is characterized in that composed of the following components:
Fish scale collagen 4%, white granulated sugar 3%, citric acid 2%, inspissated juice 15%, honey 3%, surplus are water.
3. a kind of beverage containing fish scale collagen according to claim 1 or 2, which is characterized in that the scale collagen
Albumen is prepared by following methods:
Step 1, fish scale is put into sink, the warm water of 10 times of fish scale weight is added, 40 DEG C of water temperature is impregnated 1 hour so that fish scale
Rehydration;
Step 2, treated fish scale is added to the 0.5% salt acid soak 2 hours of 10 times of fish scale weight, 40 DEG C of temperature;
Step 3, the solution immersed with fish scale obtained by step 2 is neutralized to neutrality with 0.2% sodium hydroxide;
Step 4, the fish scale after step 3 is washed till neutrality with clear water;
Step 5, the desalted water of 10 times of fish scale weight is added in fish scale, heating is boiled 0.5 hour;
Step 6, it filters, removes fish scale, temperature is cooled to 45 DEG C;
Step 7, the protease of 0.1% mass is added in solution after the cooling period, keeps the temperature 5 hours;Protease be trypsase and
The ratio of the composition of papain, the two is 1:1.
Step 8, Hydrolyzed Collagen solution made from step 7 is filtered off into removing protein enzyme using diatomaceous earth filter;
Step 9, the Hydrolyzed Collagen solution that protease is removed in step 8 is further filtered using ultrafilter;Ultrafilter
Ultrafiltration membrane restriction molecule amount is 5000 dalton
Step 10, the salt ion in filtrate that step 9 obtains is got rid of using ion exchange resin;
Step 11, it is dried using spray drying process after concentration, the collagen protein powder of finished product.
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CN201810173996.4A CN108464415A (en) | 2018-03-02 | 2018-03-02 | A kind of beverage containing fish scale collagen |
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CN201810173996.4A CN108464415A (en) | 2018-03-02 | 2018-03-02 | A kind of beverage containing fish scale collagen |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN113057337A (en) * | 2021-04-25 | 2021-07-02 | 海南三元星生物科技股份有限公司 | Functional food rich in collagen polypeptide and preparation method thereof |
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2018
- 2018-03-02 CN CN201810173996.4A patent/CN108464415A/en active Pending
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN113057337A (en) * | 2021-04-25 | 2021-07-02 | 海南三元星生物科技股份有限公司 | Functional food rich in collagen polypeptide and preparation method thereof |
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