CN108452418A - A kind of tracheal catheter and its preparation method and application being loaded with antibacterial peptide MDC coatings - Google Patents
A kind of tracheal catheter and its preparation method and application being loaded with antibacterial peptide MDC coatings Download PDFInfo
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- CN108452418A CN108452418A CN201810054269.6A CN201810054269A CN108452418A CN 108452418 A CN108452418 A CN 108452418A CN 201810054269 A CN201810054269 A CN 201810054269A CN 108452418 A CN108452418 A CN 108452418A
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- antibacterial peptide
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M16/00—Devices for influencing the respiratory system of patients by gas treatment, e.g. mouth-to-mouth respiration; Tracheal tubes
- A61M16/04—Tracheal tubes
- A61M16/0402—Special features for tracheal tubes not otherwise provided for
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L29/00—Materials for catheters, medical tubing, cannulae, or endoscopes or for coating catheters
- A61L29/14—Materials characterised by their function or physical properties, e.g. lubricating compositions
- A61L29/16—Biologically active materials, e.g. therapeutic substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L29/00—Materials for catheters, medical tubing, cannulae, or endoscopes or for coating catheters
- A61L29/08—Materials for coatings
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L29/00—Materials for catheters, medical tubing, cannulae, or endoscopes or for coating catheters
- A61L29/08—Materials for coatings
- A61L29/085—Macromolecular materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M16/00—Devices for influencing the respiratory system of patients by gas treatment, e.g. mouth-to-mouth respiration; Tracheal tubes
- A61M16/04—Tracheal tubes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/252—Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
- A61L2300/606—Coatings
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M2205/00—General characteristics of the apparatus
- A61M2205/02—General characteristics of the apparatus characterised by a particular materials
- A61M2205/0205—Materials having antiseptic or antimicrobial properties, e.g. silver compounds, rubber with sterilising agent
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M2205/00—General characteristics of the apparatus
- A61M2205/02—General characteristics of the apparatus characterised by a particular materials
- A61M2205/0238—General characteristics of the apparatus characterised by a particular materials the material being a coating or protective layer
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M2207/00—Methods of manufacture, assembly or production
Abstract
The invention belongs to biomedical materials fields, and in particular to a kind of tracheal catheter and its preparation method and application being loaded with antibacterial peptide MDC coatings.The tracheal catheter is fixed and sustained-release matrix with chitosan gel using antibacterial peptide MDC as antiseptic, carries out antibacterial surface coating to tracheal catheter, the tracheal catheter for being loaded with antibacterial peptide MDC coatings is made.Antibacterial peptide MDC sequences such as SEQ ID NO：Shown in 1.The tracheal catheter provided by the invention for being loaded with antibacterial peptide MDC coatings has significant anti-microbial property, can effectively prevent bacteria planting and growth.Therefore, the tracheal catheter provided by the invention for being loaded with antibacterial peptide MDC coatings is applied in artificial trachea cannula and lung ventilator, the generation of trachea cannula Ventilator Associated Pneumonia will be reduced or delays, the body and mind pain for reducing medical economy burden, mitigating patient has bright prospects.
Description
Technical field
The invention belongs to biomedical materials fields, and in particular to a kind of tracheal catheter being loaded with antibacterial peptide MDC coatings and
Preparation method and application.
Background technology
Trachea cannula is the simple and direct effective method that unobstructed air flue is established in critical patients rescue, and trachea cannula is established
Artificial airway, become most important one " lifeline " with patient.Need progress trachea cannula machinery logical in clinic daily
The patient of gas is countless, and the implantation material infections relating in complication becomes clinician's urgent problem to be solved.Machinery is logical
Most common and most serious complication-Ventilator Associated Pneumonia during gas, incidence, case fatality rate are high, once occur again
Less than effective control, patient, which will have to face, pulls out tracheal catheter, again intubation or tracheotomy, considerably increases patient and controls
It treats risk and body and mind torments, medical economy burden will also be significantly greatly increased.The anti-infective therapy that takes at present, air flue humidification, effectively
There is no the generations for reducing Ventilator Associated Pneumonia well for the measures such as nursing.Studies have shown that breathing caused by trachea cannula
Road infect and the common pathogen of Ventilator Associated Pneumonia be escherichia coli, Acinetobacter bauamnnii, staphylococcus aureus,
Pseudomonas aeruginosa, Klebsiella Pneumoniae etc., and these bacteriums in intubation can in succession or overlapping occur, table
Reveal multidrug resistant even general drug resistance, especially bacterial biofilm once being formed, the barrier action due to biomembrane and biology
In film the features such as bacterium low metabolism, drug resistance can be improved hundreds and thousands of times so that curative effect causes anxiety, and patient is usually due to that can not have
Effect control respiratory tract infection and it is dead.
Currently, the antibacterial surface that sight is focused on trachea cannula conduit by domestic and foreign scholars is modified, do not change original
Conduit shape makes it obtain anti-microbial property, reduces bacterial growth, Ventilator Associated Pneumonia is prevented with this.Publication No. CN
A kind of Chinese patent application " medical endotracheal tube with safe and efficient anti-microbial property " of 103948973A is with ethyl orthosilicate
For silicon source, with gamma-methyl allyl acyloxypropyl trimethoxysilane, methyltriethoxysilane for organic phase precursor, with nothing
Machine nano silver is that through sol-gel method organosilicon Ag-carried antibacterial colloidal sol is made, then using trachea cannula conduit as base in antiseptic
Material carries out antibacterial surface coating, organosilicon Ag-carried antibacterial coating trachea cannula conduit is prepared, which not only has
Efficient antibiotic property, and coating silver ion is hardly precipitated, the advantages such as surface is smooth not to adhere to sputum, and side reaction is extremely low, symbol
Close the needs of clinical trachea cannula.The Chinese patent application of Publication No. CN 103933617A is " a kind of with anti-bacterial attachment
The preparation method of tracheal catheter " is changed by preparing the colloidal sol of photocatalysis antibacterial containing iodine, carrying out surface hydroxylation to PVC tracheal catheters
Property, on PVC tracheal catheters surface form photocatalysis antibacterial membrane modifying, the tracheal catheter that keeps surface modified has good
Anti-bacterial attachment performance and visible light-inducing sterilization ability, durable antibacterial effect, chemical stability it is high.
However, the preparation method that above-mentioned published tracheal catheter antibacterial surface is modified is more complicated, and it is all made of inorganic
Chemical classes antiseptic, there are some potential safety problemss, it is difficult to avoid the problem that residual toxicity.
The appearance of antibacterial peptide (Antimicrobial peptides, AMP) in recent years is tracheal catheter antibacterial surface coating
Research bring new thinking.Antibacterial peptide is a kind of small molecule for resisting extraneous pathogenic infection that Immune System generates
Polypeptide is widely present in insect, plant, animal and human body, and wherein insect antimicrobial peptide cecropin is that the mankind have found earliest
A kind of antibacterial peptide.Antibacterial peptide has a series of noticeable biological activities, including antibacterial, anti-inflammatory, antiviral, anti-parasitism
Worm inhibits tumour cell and immunoregulatory activity etc..Antibacterial peptide Musca domestica cecropin (MDC) are this seminars
The areas the ORF overall length of a kind of insect antimicrobial peptide cloned from common house-fly grub fat-body cDNA library, the gene is 192bp, can be compiled
The precursor protein of 63 amino acid of code, 1~23 amino acids are to guard the signal peptide of 4 peptides ending, and mature peptide contains 40
Amino acid, early-stage study find that antibacterial peptide MDC shows extremely strong in-vitro antibacterial to many reference cultures and clinical drug-resistant bacterial strain
Activity can destroy bacterial cell membrane or act on intracellular target site across cell membrane, and mechanism of action is unique, thin to normal human
Born of the same parents' toxicity, side effect is small.
Currently, not being used to prepare trachea cannula conduit about the antimicrobial component using antibacterial peptide MDC as biological coating
Relevant report.
Invention content
In order to solve the problems existing in the prior art, the purpose of the present invention is to provide a kind of antibacterial peptide MDC coatings of being loaded with
Tracheal catheter and preparation method thereof.
It is another object of the present invention to provide the tracheal catheters for being loaded with antibacterial peptide MDC coatings in artificial trachea
Application in intubation and lung ventilator.
To achieve the goals above, the present invention is achieved by the following technical programs：
Antibacterial peptide MDC provided by the invention, amino acid sequence such as SEQ ID NO:Shown in 1, specifically, the amino
Acid sequence is：GWLKKI GKKIE RVGQH TRDAT IQTIG VAQQA ANVAA TLKG.
Antibacterial peptide MDC provided by the invention is made using solid-state chemical reaction method method, specifically, is closed by Peptide synthesizer
At polypeptide crude product；Then solid-phase synthesis synthesis polypeptide is used；The polypeptide of synthesis is carried out using reversed-phase high performance liquid chromatography again pure
Change, and the polypeptide of synthesis is identified using electrospray mass spectrometry, to complete the preparation of polypeptide.
The present invention also provides a kind of preparation methods of the tracheal catheter being loaded with antibacterial peptide MDC coatings comprising with
Lower step：
(1) medical chitose is dissolved in water for injection, the coagulant liquid that mass-volume concentration is 3~5% is made, is then added
A certain amount of antibacterial peptide solution makes the final concentration of 0.2mg/mL of antibacterial peptide, mixed with 300 revs/min of speed stirring at 30 DEG C
Even, prepares coating film liquid, at room temperature, with supersonic oscillations 20 minutes, the bubble removed in coating film liquid was several to get antibacterial peptide
Tetrose composite membrane liquid；
(2) natural by antibacterial peptide chitosan composite membrane liquid dip-coating made from step (1) or curtain coating in tracheal catheter base material
Drying or 50 DEG C of solidifications are dry to get tracheal catheter.
It is effective as antibacterial using chitosan gel as fixed and sustained-release matrix, antibacterial peptide MDC in above-mentioned preparation method
Coating film liquid is made in ingredient, and the chitosan is the high-molecular compound chitin (chitin) purified by crab shell, through de-
A kind of N-acetyl group manufactured poly glucosamine after deep processing again is that a kind of having good biocompatibility, biodegradable
The medical high polymer polysaccharose substance of property and biological activity.Further medical chitose gel obtained it is nontoxic, it is nonirritant,
Apyrogenetity, without challeng, haemolysis and there is no thermal stability, the adverse reactions such as no mutagenesis, lethal, also slight suppression
Bacterium acts on, and fixes in the present invention as antibacterial peptide MDC and sustained-release matrix, makes its long-term antibacterial effect of performance.
The present invention chooses antibacterial peptide MDC as biological coating, so that the surface of trachea cannula conduit is obtained anti-microbial property, effectively
Prevent bacteria planting and growth, will reduce or delay the generation of trachea cannula Ventilator Associated Pneumonia, when elongate catheter indwelling
Between, strive for the quality time for patient's rescue, improves treatment success rate, the body and mind that can also reduce medical economy burden, mitigate patient
Pain.In addition, the tracheal catheter for being loaded with antibacterial peptide MDC coatings of R ＆ D design of the present invention, inanimate object adverse reaction meet clinic
The needs of trachea cannula, are with a wide range of applications.
Compared with prior art, the invention has the advantages that：
(1) there is the tracheal catheter provided by the invention for being loaded with antibacterial peptide MDC coatings significant antibiotic property, sterilizing rate to reach
98% or more, the antibacterial peptide MDC mechanism of action is unique, is not likely to produce drug resistance, breaks through traditional antibacterials and is given birth to bacterium
The limitation of object film effect provides the tracheal catheter with safe and efficient anti-microbial property for clinic.
(2) preparation process of the tracheal catheter provided by the invention for being loaded with antibacterial peptide MDC coatings is simple, safe, nothing
Adverse reaction has a extensive future.
Description of the drawings
Fig. 1 is the high-efficient liquid phase chromatogram of antibacterial peptide MDC.
Fig. 2 is the mass spectrogram of antibacterial peptide MDC.
Fig. 3 is scanning electron microscopic observation blank control tracheal catheter surface Acinetobacter bauamnnii biomembrane morphosis.
Fig. 4 is scanning electron microscopic observation chitosan gel controls group tracheal catheter surface Acinetobacter bauamnnii biomembrane form knot
Structure.
Fig. 5 is scanning electron microscopic observation antibacterial peptide MDC chitosan gel composite coating tracheal catheters surface Acinetobacter bauamnnii
Biomembrane morphosis.
Specific implementation mode
The present invention is made with specific embodiment with reference to the accompanying drawings of the specification and further being elaborated, the embodiment
It is served only for explaining the present invention, be not intended to limit the scope of the present invention.Test method used in following embodiments is such as without spy
Different explanation, is conventional method；Used material, reagent etc., unless otherwise specified, for the reagent commercially obtained
And material.
1 solid-state chemical reaction method method synthetic antibacterial peptide MDC of embodiment
The amino acid sequence of antibacterial peptide MDC is：Ac-GWLKKI GKKIE RVGQH TRDAT IQTIG VAQQA ANVAA
TLKG-NH2, such as SEQ ID NO:Shown in 1, contain 40 amino acid, theoretical isoelectric point is 10.56, and theoretical molecular weight is
4299.04.The preparation of antibacterial peptide MDC carries out one by one from C-terminal to N-terminal, is completed by Peptide synthesizer, the specific steps are：
(1) Fmoc-X (X is first amino acid of antibacterial peptide MDC C-terminals) is linked into Wang resins first, then taken off
X-Wang resins are obtained after removing Fmoc groups；By Fmoc-Y-Trt-OH, (9- fluorenes methoxy carboxyl-trimethyl-Y, Y is antibacterial peptide again
Second amino acid of MDC C-terminals)；It is synthesized to N-terminal from C-terminal successively according to this program, until synthesis finishes, obtains sloughing Fmoc
The resin of the side chain protection of group；
(2) in peptide resin obtained above, cutting reagent is added, 20 DEG C are protected from light lower reaction 2h, filtering；Precipitation TFA
(trifluoroacetic acid) washs, and washing lotion is mixed with above-mentioned filtrate, and Rotary Evaporators concentration adds the precooling nothing of 10 times or so volumes
Water ether, -20 DEG C of precipitation 3h, is precipitated white powder object, centrifuges 10min with 2500g, collects precipitation, then washed with anhydrous ether
Precipitation, vacuum drying, obtains polypeptide, wherein cutting reagent by TFA, water and TIS (tri isopropyl chlorosilane) according to mass ratio 95:
2.5:2.5 mixing；
(3) 0.2mol/L sodium sulphate (phosphoric acid is adjusted to pH7.5) is used to carry out column equilibration 30min, with 90% aqueous acetonitrile
Liquid dissolves polypeptide, and filtering, C18 reverse phase normal pressure columns, using gradient elution, (eluant, eluent is methanol and aqueous sodium persulfate solution according to volume
Than being 30:70~70:30 mixing), flow velocity 1mL/min, detection wave is 220nm, collects main peak, freeze-drying；
(4) reverse phase C18 columns are recycled to be further purified, eluent A is 0.1%TFA/ aqueous solutions；Eluent B is 0.1%
TFA/ acetonitrile solutions, wash-out concentration is 25%B~40%B, elution time 12min, flow velocity 1mL/min, then is ibid collected
Main peak is lyophilized to get refined antibacterial peptide MDC；
(5) refined antibacterial peptide MDC is obtained by reversed-phase high performance liquid chromatography and electron spray mass spectrometry analysis, reverse phase by above-mentioned
High-efficient liquid phase chromatogram as shown in Figure 1, mass spectrogram as shown in Fig. 2, the results show that the purity of antibacterial peptide MDC be more than 95%, molecule
Amount is 4299.36, almost the same with theoretical molecular weight.
It is prepared by the tracheal catheter that embodiment 2 is loaded with antibacterial peptide MDC coatings
(1) medical chitose is dissolved in water for injection, the coagulant liquid that mass-volume concentration is 4% is made, is then added one
Quantitative antibacterial peptide solution is made the final concentration of 0.2mg/mL of antibacterial peptide, is stirred and evenly mixed with 300 revs/min of speed at 30 DEG C,
Prepares coating film liquid with supersonic oscillations 20 minutes, removes the bubble in coating film liquid to get antibacterial peptide chitin at room temperature
Sugared composite membrane liquid；
(2) by antibacterial peptide chitosan composite membrane liquid dip-coating made from step (1) or curtain coating in PVC tracheal catheter base materials,
Natural drying or 50 DEG C of solidifications are dried to get the tracheal catheter of antibacterial peptide MDC coatings is loaded with.
Test example one, safety evaluatio
(1) cell toxicity test
According to cell toxicity test method as defined in GB/T16886.5-2003, to being loaded with the tracheae of antibacterial peptide MDC coatings
Catheter samples carry out cell toxicity test.The tracheae for being loaded with antibacterial peptide MDC coatings is prepared with 10% calf serum MEM culture mediums
Catheter samples leaching liquor culture NCTC colone929 cells (l cell), and with blank PVC trachea cannula conduits
Sample leaching liquor makees negative control, and 5g/L phenol solutions make positive control.
Test result：The cellular morphology of negative control is normal, and adherent growth is good, there is discrete particle in endochylema, acellular
Dissolving；The cell growth of positive control is bad, and 90% or more cell is rounded or cell dissolution；It is loaded with antibacterial peptide MDC coatings
Cellular morphology is normal in tracheal catheter sample leaching liquor, and adherent growth is good, there is discrete particle, acellular dissolving in endochylema.It carries
There are the tracheal catheter sample of antibacterial peptide MDC coatings and blank PVC trachea cannula conduit sample leaching liquors to be all chosen as nontoxic, judgement point
Grade is 0 grade (it is qualified that cell-cytotoxic reaction, which is 0 grade or 1 grade).48 hours monitoring cell OD570 of culture and opposite increment degree, carry
The tracheal catheter sample and blank PVC trachea cannula conduit samples leaching liquor for having antibacterial peptide MDC coatings all judge to be classified as 0 grade,
The tracheal catheter sample cell toxicity test result for being loaded with antibacterial peptide MDC coatings is qualification.
(2) acute systemic toxicity
According to GB/T16886.11-2011 standards, observation is loaded with the tracheal catheter sample of antibacterial peptide MDC coatings to mouse urgency
Property general toxic reaction.Blank PVC trachea cannula conduits sample and the tracheal catheter sample for being loaded with antibacterial peptide MDC coatings
0.9% sodium chloride injection leaching liquor is given in a single dose in a manner of tail vein injection, and accordingly to extract medium as blank control.
4,24,48 and 72h after injection observes mouse toxicity response situation.As a result, it has been found that：Blank control liquid group, blank PVC trachea cannulas
Catheter samples and the tracheal catheter sample leaching liquor group for being loaded with antibacterial peptide MDC coatings are showed no abnormal response.The result shows that：It is loaded with
The acute systemic toxicity result of the tracheal catheter sample leaching liquor of antibacterial peptide MDC coatings is to meet non-toxic requirement, and empty
The acute systemic toxicity indifference of white PVC trachea cannula conduit samples.
Test example two, antibiotic property evaluation
Using Acinetobacter bauamnnii as representative, simulation clinic ICU Ventilator Acquired Acinetobacter bauamnnii biomembranes infection is built
Vertical Acinetobacter bauamnnii biomembrane external model.Using blank tracheal catheter sample without any processing as blank control group, together
When set chitosan gel controls group, liquid flowing state in simulated respiration machine conduit, each group tracheal catheter is impregnated in 1.0 ×
105In Acinetobacter bauamnnii bacterium solution, culture for 24 hours, takes 10ul maceration extracts to be coated with inoculation on LB culture mediums, 35 DEG C are incubated overnight
Afterwards, clump count is counted, the relative growth situation of bacterium is observed, to show its anti-microbial property.
The result of contrast experiment confirms：Blank tracheal catheter sample bacterium normal growth, chitosan gel controls group tracheae
Catheter samples bacterial growth reduces unobvious, and sterilizing rate is 5% or so, and antibacterial peptide MDC chitosan gel composite coating tracheaes
Catheter samples bacterial growth, which significantly reduces, even to disappear, and sterilizing rate is up to 98% or more.
Wherein, scanning electron microscopic observation tracheal catheter surface biological film morphosis is as shown in Figure 3-Figure 5, as a result shows：It is empty
The white visible a large amount of bacterial adhesions of tracheal catheter (Fig. 3) that compare are agglomerating, form big blocky biomembrane；Chitosan gel controls group tracheae
Conduit (Fig. 4) biofilm thickness and area are reduced；Antibacterial peptide MDC chitosan gel composite coating tracheal catheters (Fig. 5)
It is rarely seen to be dispersed in bacterium and bacterium cell deforms, is broken, outer membrane is detached from, content leaks；
In addition, agar plate colony counting method result is also shown：Antibacterial peptide MDC chitosan gel composite coating tracheal catheters
Surface viable count is substantially reduced compared with blank control group and chitosan gel controls group.
It the above is only the preferred embodiment of the present invention, it is noted that above-mentioned preferred embodiment is not construed as pair
The limitation of the present invention, protection scope of the present invention should be subject to claim limited range.For the art
For those of ordinary skill, without departing from the spirit and scope of the present invention, several improvements and modifications can also be made, these change
Protection scope of the present invention is also should be regarded as into retouching.
Sequence table
<110>Guangdong pharmaceutical university
<120>A kind of tracheal catheter and its preparation method and application being loaded with antibacterial peptide MDC coatings
<130> 1122
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 40
<212> PRT
<213>Artificial sequence (Artificial Sequence)
<400> 1
Gly Thr Leu Leu Leu Ile Gly Leu Leu Ile Gly Ala Val Gly Gly His
1 5 10 15
Thr Ala Ala Ala Thr Ile Gly Thr Ile Gly Val Ala Gly Gly Ala Ala
20 25 30
Ala Val Ala Ala Thr Leu Leu Gly
35 40
Claims (6)
1. a kind of tracheal catheter, which is characterized in that the tracheal catheter surface is loaded with antibacterial peptide MDC as biological coating.
2. tracheal catheter according to claim 1, which is characterized in that the amino acid sequence such as SEQ of the antibacterial peptide MDC
ID NO:Shown in 1.
3. the preparation method of tracheal catheter according to claim 1 or 2, which is characterized in that include the following steps：
(1) medical chitose is dissolved in water for injection, the coagulant liquid that mass-volume concentration is 3~5% is made, be then added certain
The antibacterial peptide solution of amount is made the final concentration of 0.2mg/mL of antibacterial peptide, is stirred and evenly mixed with 300 revs/min of speed at 30 DEG C, system
Standby coating film liquid with supersonic oscillations 20 minutes, removes the bubble in coating film liquid to get antibacterial peptide chitosan at room temperature
Composite membrane liquid；
(2) by antibacterial peptide chitosan composite membrane liquid dip-coating made from step (1) or curtain coating in tracheal catheter base material, natural drying
Or 50 DEG C of solidifications are dry to get tracheal catheter.
4. the preparation method of tracheal catheter according to claim 3, which is characterized in that the tracheal catheter base material is poly-
Vinyl chloride.
5. according to application of any tracheal catheters of claim 1-4 in artificial trachea cannula and lung ventilator.
6. a kind of antibacterial peptide MDC, amino acid sequence such as SEQ ID NO:Shown in 1.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810054269.6A CN108452418A (en) | 2018-01-19 | 2018-01-19 | A kind of tracheal catheter and its preparation method and application being loaded with antibacterial peptide MDC coatings |
| PCT/CN2018/083402 WO2019140796A1 (en) | 2018-01-19 | 2018-04-17 | Endotracheal tube loaded with antimicrobial peptide mdc coating and preparation method therefor and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810054269.6A CN108452418A (en) | 2018-01-19 | 2018-01-19 | A kind of tracheal catheter and its preparation method and application being loaded with antibacterial peptide MDC coatings |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108452418A true CN108452418A (en) | 2018-08-28 |
Family
ID=63221009
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810054269.6A Pending CN108452418A (en) | 2018-01-19 | 2018-01-19 | A kind of tracheal catheter and its preparation method and application being loaded with antibacterial peptide MDC coatings |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN108452418A (en) |
| WO (1) | WO2019140796A1 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111973805A (en) * | 2019-08-16 | 2020-11-24 | 苏州吉美瑞生医学科技有限公司 | Application of antibacterial peptide hCAP18/LL-37 in anti-infection bioengineering lung |
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| CN115666237A (en) * | 2020-03-27 | 2023-01-31 | 山尼饮料责任有限公司 | Antibacterial tubular catheter |
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| WO2019140796A1 (en) | 2019-07-25 |
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