CN108450648A - A kind of extracting method of grub biological alkali and its application - Google Patents

A kind of extracting method of grub biological alkali and its application Download PDF

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Publication number
CN108450648A
CN108450648A CN201810192372.7A CN201810192372A CN108450648A CN 108450648 A CN108450648 A CN 108450648A CN 201810192372 A CN201810192372 A CN 201810192372A CN 108450648 A CN108450648 A CN 108450648A
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grub
parts
biological alkali
powder
extracting method
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不公告发明人
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Guangzhou Poly Health Industry Research Institute Co Ltd
Foshan Harvest Chemical Technology Co Ltd
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Guangzhou Poly Health Industry Research Institute Co Ltd
Foshan Harvest Chemical Technology Co Ltd
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Publication of CN108450648A publication Critical patent/CN108450648A/en
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/50—Feeding-stuffs specially adapted for particular animals for rodents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56—Materials from animals other than mammals
    • A61K35/63—Arthropods
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/55—Liquid-liquid separation; Phase separation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

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  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
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  • Health & Medical Sciences (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
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  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Insects & Arthropods (AREA)
  • Birds (AREA)
  • Pharmacology & Pharmacy (AREA)
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  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
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Abstract

The invention discloses a kind of extraction process of grub biological alkali and its applications, further include a kind of feed.The extraction process of the grub biological alkali, mainly there is following steps：Clean dry crushes, and ultrasonic extraction is concentrated and extracted, and is finally separating purification.The present invention extracts utilization by relatively mild means to grub biological alkali, and by keeping alkaloid activity to greatest extent, preferable antibacterial and anti-inflammation functions can be played by so that it is added in feed.

Description

A kind of extracting method of grub biological alkali and its application
Technical field
The present invention relates to a kind of extracting methods of alkaloid, and in particular to a method of extracting alkaloid from grub.
Background technology：
Grub also known as hen worm, grub, one's old mother worm, worm etc. when white, build is loose, common name scarab beetle or cockchafer after adult Son.
Alkaloid is the nitrogenous organic compound of one kind in nature, is mostly alkalinity, has significant bioactivity. In daily use, including in a large amount of fields such as medicine, biology, pesticide, cosmetics, alkaloid is closed as new type resource by emphasis Note.And alkaloid is extracted, research at present focuses mostly in plant extract, or is extracted from the toxin of marine organisms, right Lack people in the alkaloid in insect bodies to make inquiries.
Insect alkaloid has antibacterial, anti-inflammatory, antiviral, anti-parasitism and other effects, can be reduced by adjusting immunologic balance The influence of other foreign substances.And insect is also more convenient than plant and marine organisms in raising, extraction and application insect alkaloid has Inborn advantage.
But the extraction and application actually about grub biological alkali, there is no relevant report.
Invention content：
The method that the object of the present invention is to provide a kind of to extract alkaloid from grub, to solve the above technical problems. The present invention extracts utilization by relatively mild means to grub biological alkali, by keeping alkaloid activity to greatest extent, It can be made to be added in feed and play preferable antibacterial and anti-inflammation functions.
To achieve the above object, the present invention provides following scheme：
The present invention provides a kind of method for extracting alkaloid from grub, includes the following steps：
(1) by grub cleaning, drying, 40~60 mesh sieve is crushed, grub powder is obtained；
(2) the grub powder of step (1) is taken, 85~95% ethyl alcohol are added, then 30~40min of ultrasonic extraction, is filtered, filter Slag repeats extraction 2~3 times, merging filtrate；
(3) filtrate of step (2) is adjusted into pH to 10~12 with weak aqua ammonia, no alcohol is concentrated under reduced pressure at 50~70 DEG C Taste obtains medicinal extract；
(4) by appropriate water-dispersed at suspension, the ethyl acetate extraction that then 3~5 times of addition is measured of the medicinal extract of step (3) It takes 3~4 times, removes oil-soluble impurities, collect water layer；
(5) by 3~5 times of the water layer of step (4) the addition extracting n-butyl alcohol measured 3~5 times, n-butanol is recovered under reduced pressure, obtains Grub biological alkali；
Further, the mass volume ratio (g/mL) of grub powder and ethyl alcohol is 1 in the step (2)：(10~15).
Further, ultrasonic extraction conditions in the step (2)：Power is 100~200W, and temperature is 45~60 DEG C.
Further, the grub biological alkali can be applied in feed.
Further, the feed is imperial cat feed.
Further, the present invention also provides a kind of imperial cat feed, include the component of following parts by weight：Corn flour 15~25 Part, 1~3 part of grub biological alkali, 1~3 part of fig polysaccharide, 15~20 parts of dregs of beans, 5~15 parts of soybean peptide, carrot meal 5~15 Part, 3~8 parts of saussurea involucrata fruit powder, 0.1~1 part of multi-vitamins, 0.1~1 part of minerals, 1~3 part of honey.
Compared with prior art, the present invention has following advantageous effect：
(1) in extraction step of the invention, the condition used is more mild, so that the destruction that glycosidic bond is subject to is reduced, therefore energy Obtain the higher grub biological alkali of quality；
(2) in extraction process of the invention, using ethyl alcohol as solvent, the premise for destroying damage grub biological alkali is being reduced Under, grub biological alkali can be preferably dissolved, simultaneously because ethyl alcohol is easy to be recovered under reduced pressure, so that product is more preferably extracted and divides From；Meanwhile under the auxiliary of ultrasound, solvent molecule movement is accelerated, and preferably promotes the dissolving diffusion of grub biological alkali；
(3) by the way that grub biological alkali to be applied in imperial cat feed, the effect of feed antibacterial anti-inflammatory can be assigned.
Specific implementation mode
With reference to specific embodiment, the present invention is described in further detail.
Embodiment 1
A method of it extracting alkaloid from grub, includes the following steps：
(1) by grub cleaning, drying, 50 mesh sieve is crushed, grub powder is obtained；
(2) the grub powder of step (1) is taken, 90% ethyl alcohol, the mass volume ratio (g/mL) of grub powder and ethyl alcohol is added It is 1：12, then power be 150W, 53 DEG C under the conditions of ultrasonic extraction 35min, filtering, filter residue repeat extraction 2 times, merge filter Liquid；
(3) filtrate of step (2) is adjusted into pH to 11 with weak aqua ammonia, no alcohol taste is concentrated under reduced pressure at 60 DEG C, is soaked Cream；
(4) by appropriate water-dispersed at suspension, the ethyl acetate extraction 3 that then 4 times of addition is measured of the medicinal extract of step (3) It is secondary, oil-soluble impurities are removed, water layer is collected；
(5) by 4 times of the water layer of step (4) the addition extracting n-butyl alcohol measured 4 times, n-butanol is recovered under reduced pressure, obtains grub life Alkaloids.
Embodiment 2
A method of it extracting alkaloid from grub, includes the following steps：
(1) by grub cleaning, drying, 60 mesh sieve is crushed, grub powder is obtained；
(2) the grub powder of step (1) is taken, 85% ethyl alcohol, the mass volume ratio (g/mL) of grub powder and ethyl alcohol is added It is 1：10, then power be 100W, 45 DEG C under the conditions of ultrasonic extraction 40min, filtering, filter residue repeat extraction 3 times, merge filter Liquid；
(3) filtrate of step (2) is adjusted into pH to 12 with weak aqua ammonia, no alcohol taste is concentrated under reduced pressure at 50 DEG C, is soaked Cream；
(4) by appropriate water-dispersed at suspension, the ethyl acetate extraction 3 that then 3 times of addition is measured of the medicinal extract of step (3) It is secondary, oil-soluble impurities are removed, water layer is collected；
(5) by 5 times of the water layer of step (4) the addition extracting n-butyl alcohol measured 5 times, n-butanol is recovered under reduced pressure, obtains grub life Alkaloids.
Embodiment 3
A method of it extracting alkaloid from grub, includes the following steps：
(1) by grub cleaning, drying, 40 mesh sieve is crushed, grub powder is obtained；
(2) the grub powder of step (1) is taken, 95% ethyl alcohol, the mass volume ratio (g/mL) of grub powder and ethyl alcohol is added It is 1：15, then power be 200W, 60 DEG C under the conditions of ultrasonic extraction 30min, filtering, filter residue repeat extraction 2 times, merge filter Liquid；
(3) filtrate of step (2) is adjusted into pH to 10 with weak aqua ammonia, no alcohol taste is concentrated under reduced pressure at 70 DEG C, is soaked Cream；
(4) by appropriate water-dispersed at suspension, the ethyl acetate extraction 4 that then 5 times of addition is measured of the medicinal extract of step (3) It is secondary, oil-soluble impurities are removed, water layer is collected；
(5) by 3 times of the water layer of step (4) the addition extracting n-butyl alcohol measured 3 times, n-butanol is recovered under reduced pressure, obtains grub life Alkaloids.
Embodiment 4
A kind of dragon cat feed, includes the component of following parts by weight：25 parts of corn flour, 1 part of grub biological alkali, fig polysaccharide 3 parts, 20 parts of dregs of beans, 5 parts of soybean peptide, 5 parts of carrot meal, 3 parts of saussurea involucrata fruit powder, 1 part of multi-vitamins, 0.1 part of minerals, honey 1 Part.
Embodiment 5
A kind of dragon cat feed, includes the component of following parts by weight：15 parts of corn flour, 3 parts of grub biological alkali, fig polysaccharide 1 part, 15 parts of dregs of beans, 15 parts of soybean peptide, 15 parts of carrot meal, 8 parts of saussurea involucrata fruit powder, 0.1 part of multi-vitamins, 1 part of minerals, bee 3 parts of honey.
Comparative example 1
A method of it extracting alkaloid from grub, includes the following steps：
(1) by grub cleaning, drying, 50 mesh sieve is crushed, grub powder is obtained；
(2) the grub powder of step (1) is taken, 90% petroleum ether, the mass volume ratio (g/ of grub powder and petroleum ether is added ML it is) 1：12, then power be 150W, 53 DEG C under the conditions of ultrasonic extraction 35min, filtering, filter residue repeat extraction 2 times, merge Filtrate；
(3) filtrate of step (2) is adjusted into pH to 11 with weak aqua ammonia, no alcohol taste is concentrated under reduced pressure at 60 DEG C, is soaked Cream；
(4) by appropriate water-dispersed at suspension, the ethyl acetate extraction 3 that then 4 times of addition is measured of the medicinal extract of step (3) It is secondary, oil-soluble impurities are removed, water layer is collected；
(5) by 4 times of the water layer of step (4) the addition extracting n-butyl alcohol measured 4 times, n-butanol is recovered under reduced pressure, obtains grub life Alkaloids.
Comparative example 2
A method of it extracting alkaloid from grub, includes the following steps：
(1) by grub cleaning, drying, 50 mesh sieve is crushed, grub powder is obtained；
(2) the grub powder of step (1) is taken, 90% ethyl alcohol, the mass volume ratio (g/mL) of grub powder and ethyl alcohol is added It is 1：12, refluxing extraction, filtering, filter residue repetition extraction 2 times, merging filtrate；
(3) filtrate of step (2) is adjusted into pH to 11 with weak aqua ammonia, no alcohol taste is concentrated under reduced pressure at 60 DEG C, is soaked Cream；
(4) by appropriate water-dispersed at suspension, the ethyl acetate extraction 3 that then 4 times of addition is measured of the medicinal extract of step (3) It is secondary, oil-soluble impurities are removed, water layer is collected；
(5) by 4 times of the water layer of step (4) the addition extracting n-butyl alcohol measured 4 times, n-butanol is recovered under reduced pressure, obtains grub life Alkaloids.
Grub biological alkali carries taken amount is evaluated
Preferably to prove that it is excellent that a kind of grub biological alkali extracting method provided by the present invention has the effect of, choose real It applies example 1,2,3 and the extracting method of comparative example 1,2 extracts grub biological alkali respectively, following table institute is carried out to grub biological alkali carries taken amount State measurement.
Table 3：The performance test results of embodiment 1-3 and comparative example 1-2：
The result shows that：
From table 3 it is observed that the grub biological alkali carries extracted by the grub biological alkali extracting method of the present invention Taken amount is higher, and is better than comparative example.Using petroleum ether extraction comparative example 1 compared with Example 1, grub biological alkali carries taken amount It is significant lower, show, using ethyl alcohol as solvent, under the premise of reducing destruction damage grub biological alkali, can preferably dissolve grub Alkaloid, simultaneously because ethyl alcohol is easy to be recovered under reduced pressure, so that product is more preferably extracted and detaches；Ultrasonic extraction is not used Compared with Example 1, grub biological alkali carries taken amount is significant lower for comparative example 2；Show under the auxiliary of ultrasound, solvent molecule movement Accelerate, preferably promotes the dissolving diffusion of grub biological alkali, to obtain higher extracted amount.
The present invention is described by way of example above, but the present invention is not limited to above-mentioned specific embodiment, all to be based on Any changes or modifications that the present invention is done belong to the scope of protection of present invention.

Claims (6)

1. a kind of extracting method of grub biological alkali, which is characterized in that include the following steps：
(1) by grub cleaning, drying, 40~60 mesh sieve is crushed, grub powder is obtained；
(2) the grub powder of step (1) is taken, 85~95% ethyl alcohol are added, then 30~40min of ultrasonic extraction, filtering, filter residue weight It extracts 2~3 times again, merging filtrate；
(3) filtrate of step (2) is adjusted into pH to 10~12 with weak aqua ammonia, no alcohol taste is concentrated under reduced pressure at 50~70 DEG C, is obtained To medicinal extract；
(4) by appropriate water-dispersed at suspension, the ethyl acetate extraction 3 that then 3~5 times of addition is measured of the medicinal extract of step (3) ~4 times, oil-soluble impurities are removed, collect water layer；
(5) by 3~5 times of the water layer of step (4) the addition extracting n-butyl alcohol measured 3~5 times, n-butanol is recovered under reduced pressure, obtains grub Alkaloid.
2. extracting method according to claim 1, which is characterized in that the matter of grub powder and ethyl alcohol in the step (2) It is 1 to measure volume ratio (g/mL)：(10~15).
3. extracting method according to claim 1, which is characterized in that ultrasonic extraction conditions in the step (2)：Power is 100~200W, temperature are 45~60 DEG C.
4. according to application of the grub biological alkali in feed made from any one of claims 1 to 3 extracting method.
5. application according to claim 4, which is characterized in that the feed is imperial cat feed.
6. a kind of dragon cat feed, which is characterized in that include the component of following parts by weight：15~25 parts of corn flour, grub biological alkali 1 ~3 parts, 1~3 part of fig polysaccharide, 15~20 parts of dregs of beans, 5~15 parts of soybean peptide, 5~15 parts of carrot meal, saussurea involucrata fruit powder 3~8 Part, 0.1~1 part of multi-vitamins, 0.1~1 part of minerals, 1~3 part of honey.
CN201810192372.7A 2018-03-08 2018-03-08 A kind of extracting method of grub biological alkali and its application Pending CN108450648A (en)

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Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101623329A (en) * 2008-07-07 2010-01-13 中国科学院成都生物研究所 Method for extracting paper mulberry alkaloid and application of paper mulberry alkaloid
KR101086669B1 (en) * 2010-09-15 2011-11-24 (주)선바이오텍 Method for preparing smilax china l and cosmetic compound for skin whitening compring extract from same
CN102579506A (en) * 2012-04-09 2012-07-18 中国人民解放军第四军医大学 Preparation method and application of grub total alkaloid
CN102617583A (en) * 2012-03-08 2012-08-01 西北农林科技大学 Novel method for quickly extracting and separating five types of alkaloids from macleaya cordata
CN103251790A (en) * 2013-05-06 2013-08-21 乌鲁木齐欧易生物医学科技有限公司 Method for extracting total alkaloids from fritillaria pallidiflora
CN104814253A (en) * 2015-05-19 2015-08-05 合肥市好旺养殖科技有限公司 Yellow meal worm chinchilla feed and preparation method thereof
CN105037153A (en) * 2015-07-23 2015-11-11 山东师范大学 Method for preparing DBP through waste liquid generated by antarctic krill alkaloid extraction
CN106674086A (en) * 2016-12-27 2017-05-17 西安交通大学 Piperidone alkaloid compounds as well as preparation method and application thereof

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101623329A (en) * 2008-07-07 2010-01-13 中国科学院成都生物研究所 Method for extracting paper mulberry alkaloid and application of paper mulberry alkaloid
KR101086669B1 (en) * 2010-09-15 2011-11-24 (주)선바이오텍 Method for preparing smilax china l and cosmetic compound for skin whitening compring extract from same
CN102617583A (en) * 2012-03-08 2012-08-01 西北农林科技大学 Novel method for quickly extracting and separating five types of alkaloids from macleaya cordata
CN102579506A (en) * 2012-04-09 2012-07-18 中国人民解放军第四军医大学 Preparation method and application of grub total alkaloid
CN103251790A (en) * 2013-05-06 2013-08-21 乌鲁木齐欧易生物医学科技有限公司 Method for extracting total alkaloids from fritillaria pallidiflora
CN104814253A (en) * 2015-05-19 2015-08-05 合肥市好旺养殖科技有限公司 Yellow meal worm chinchilla feed and preparation method thereof
CN105037153A (en) * 2015-07-23 2015-11-11 山东师范大学 Method for preparing DBP through waste liquid generated by antarctic krill alkaloid extraction
CN106674086A (en) * 2016-12-27 2017-05-17 西安交通大学 Piperidone alkaloid compounds as well as preparation method and application thereof

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