CN108440248A - The extracting method of resveratrol and its application in peanut sprout - Google Patents
The extracting method of resveratrol and its application in peanut sprout Download PDFInfo
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- CN108440248A CN108440248A CN201810190066.XA CN201810190066A CN108440248A CN 108440248 A CN108440248 A CN 108440248A CN 201810190066 A CN201810190066 A CN 201810190066A CN 108440248 A CN108440248 A CN 108440248A
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- resveratrol
- peanut sprout
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C37/00—Preparation of compounds having hydroxy or O-metal groups bound to a carbon atom of a six-membered aromatic ring
- C07C37/68—Purification; separation; Use of additives, e.g. for stabilisation
- C07C37/685—Processes comprising at least two steps in series
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C37/00—Preparation of compounds having hydroxy or O-metal groups bound to a carbon atom of a six-membered aromatic ring
- C07C37/68—Purification; separation; Use of additives, e.g. for stabilisation
- C07C37/70—Purification; separation; Use of additives, e.g. for stabilisation by physical treatment
- C07C37/72—Purification; separation; Use of additives, e.g. for stabilisation by physical treatment by liquid-liquid treatment
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- Medicines Containing Plant Substances (AREA)
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Abstract
The invention discloses a kind of extracting methods of resveratrol in peanut sprout and its application, extracting method to include the following steps：Peanut sprout is smashed, is placed in hot water and impregnates, obtains mixed liquor A；Lactic acid bacteria, xylitol fermentation are added into mixed liquor A, obtains mixed liquid B；Mixed liquid B uses microwave irradiation, extracts resveratrol, obtains the extracting solution for including resveratrol.The extracting method of resveratrol in the peanut sprout, it ferments with addition lactic acid bacteria, xylitol in the peanut sprout after hot-water soak, resveratrol is extracted, keep its mouthfeel and shelf-life performance more stable, the extraction yield higher of resveratrol, extraction time and cost are lower, while also avoiding chemical agent to the intervention of resveratrol influence.In addition, the mixed liquor after fermentation process is used microwave irradiation auxiliary extraction, the recovery rate and extraction quality of resveratrol are improved, avoids extract denaturation or thermal loss, while resveratrol being avoided to be destroyed, extraction rate reached to 90% or more.
Description
Technical field
The present invention relates to the extractive technique fields of resveratrol, particularly, are related to a kind of carrying for resveratrol in peanut sprout
Take method and its application.
Background technology
Peanut germination is when growing peanut sprout, and peanut sprout will produce resveratrol in order to avoid by outside stimulus.With peanut sprout
Growth, the content of resveratrol can also be significantly increased.Studies show that, the oxidize-resistant physiology active material of peanut-sprout is outstanding
The content of its resveratrol is up to 90 times of peanut, tens times or even hundred times more than red wine.
Resveratrol is a kind of plant that plant is mouldy or naturally secret goes out out of plant when being endangered by pest
Antitoxin has control platelet aggregation, reduces serum cholesterol, effectively prevent anti-oxidant and heart disease, prevent low fat albumen
Matter aoxidizes and normal cell becomes cancer cell, prevents the pathological effects such as malignant tumour hyperplasia, anti-inflammatory (Korea J Crop
Scl., 48 (6)：429~433,2003).According to the paper that Britain Nature is delivered, resveratrol can extend intake high heat
Mouse life (Nature, vol 44 (16), 2006).But the existing method that resveratrol is extracted from peanut sprout, all
Extract denaturation or thermal loss, resveratrol can be caused to be destroyed, reduce resveratrol in peanut sprout extract
Content and be unfavorable for using as food material.Therefore the resveratrol in extraction peanut sprout, by the resveratrol containing high concentration
Extract is used for the functional food material rich in resveratrol, is a problem to be solved.
Invention content
The present invention provides a kind of extracting method of resveratrol in peanut sprout and its application, with solve in the prior art from
Extraction resveratrol is easy to cause resveratrol in extract denaturation or thermal loss, extract and is subject to destroy in peanut sprout
The technical issues of.
The technical solution adopted by the present invention is as follows：
The extracting method of resveratrol, includes the following steps in a kind of peanut sprout：(1) peanut sprout is smashed, is placed in hot water
Middle immersion, obtains mixed liquor A；(2) lactic acid bacteria, xylitol fermentation are added in the mixed liquor A obtained to the step (1), obtains
Mixed liquid B；(3) mixed liquid B made from the step (2) uses microwave irradiation, extracts resveratrol, obtains including white black false hellebore
The extracting solution of alcohol.
Further, in the step (1), the temperature of the hot water is 80~95 DEG C, and soaking time is 10~15h.
Further, in the step (1), the weight of the hot water is 2~3 times of the peanut sprout weight.
Further, in the step (2), be added the lactic acid bacteria weight be the mixed liquor A weight 5%~
7%, the weight that the xylitol is added is the 5%~7% of the mixed liquor A weight.
Further, in the step (2), fermentation temperature is 30~45 DEG C, and fermentation time is 20~25h.
Further, in the step (3), the mixed liquid B is positioned in the micro-wave oven of 800~1000M Hz and is shone
It penetrates, irradiation time is 10~15s.
Further, the extracting method of resveratrol is further comprising the steps of in peanut sprout：The step (3) is obtained
Extracting solution carries out vacuum condensation concentration, you can obtains including the concentrate of resveratrol.
Further, the temperature when vacuum condensation concentration is 80~95 DEG C, obtained described after vacuum condensation concentration
The temperature of concentrate is controlled at 40 DEG C or less.
Further, the time of the vacuum condensation concentration is 10~15h.
According to another aspect of the present invention, the extracting method for additionally providing resveratrol in a kind of above-mentioned peanut sprout is extracted
The resveratrol arrived.
According to another aspect of the present invention, the extracting method for additionally providing resveratrol in a kind of above-mentioned peanut sprout is extracted
To application of the resveratrol in drug and/or food.
The invention has the advantages that：
The extracting method of resveratrol in peanut sprout provided by the invention, newborn with being added in the peanut sprout after hot-water soak
Sour bacterium, xylitol ferment, and resveratrol is extracted, and so that its mouthfeel and shelf-life performance is more stablized, resveratrol
Extraction yield higher, extraction time and cost are lower, while also avoiding chemical agent and influenced on the intervention of resveratrol.Make
It is the Best Technical Approach of most scientific pure natural acquisition resveratrol with above-mentioned fermentation method extraction resveratrol.In addition, will
Mixed liquor after fermentation process uses microwave irradiation auxiliary extraction, improves the recovery rate and extraction quality of resveratrol, avoids
Extract is denaturalized or thermal loss, while resveratrol being avoided to be destroyed, extraction rate reached to 90% or more.The present invention uses
Peanut sprout ferments after impregnating and the technology of microwave auxiliary extraction, can improve the extraction yield and stability of resveratrol.
Other than objects, features and advantages described above, the present invention also has other objects, features and advantages.
The present invention is further detailed explanation below.
Specific implementation mode
The embodiment of the present invention is described in detail below, but what the present invention can be defined by the claims and cover
Multitude of different ways is implemented.
The preferred embodiment of the present invention provides a kind of extracting method of resveratrol in peanut sprout, includes the following steps：
(1) peanut sprout is smashed, is placed in hot water and impregnates, obtain mixed liquor A；(2) breast is added in the mixed liquor A obtained to step (1)
Sour bacterium, xylitol fermentation, obtain mixed liquid B；(3) mixed liquid B made from step (2) uses microwave irradiation, extracts white black false hellebore
Alcohol obtains the extracting solution for including resveratrol.
The extracting method of resveratrol in above-mentioned peanut sprout, in the peanut sprout after hot-water soak be added lactic acid bacteria, wood
Sugar alcohol ferments, and resveratrol is extracted, and so that its mouthfeel and shelf-life performance is more stablized, the extraction of resveratrol
Rate higher, extraction time and cost are lower, while also avoiding chemical agent to the intervention of resveratrol influence.Using above-mentioned
Fermentation method extraction resveratrol is the Best Technical Approach of most scientific pure natural acquisition resveratrol.In addition, by fermentation
Mixed liquor after reason uses microwave irradiation auxiliary extraction, improves the recovery rate and extraction quality of resveratrol, avoids extract
Denaturation or thermal loss, while resveratrol being avoided to be destroyed, extraction rate reached to 90% or more.The present invention uses peanut sprout
The technology of fermentation and microwave auxiliary extraction after immersion, can improve the extraction yield and stability of resveratrol.
Peanut sprout used in said extracted method can be the peanut sprout product of Patent No. 201210455520.2,
With 15~20cm of length, leaf, sturdy delicate organic ecosystem peanut sprout are not opened.In the present embodiment, first go root clear peanut sprout
Wash clean, and smash and be squeezed into juice, it is subsequently placed in hot water and impregnates.
Preferably, in step (1), the temperature of hot water is 80~95 DEG C, and soaking time is 10~15h.Said extracted method
In, the 10~15h of peanut sprout smashed is impregnated using 80~95 DEG C, the ingredient in peanut sprout can be made to be effectively dissolved in hot water
In, condition is provided for the extraction of resveratrol, improves the recovery rate of resveratrol, while subsequent extracted object being avoided to be denaturalized.
Preferably, in step (1), the weight of hot water is 2~3 times of peanut sprout weight.In said extracted method, hot water with
The preferred weight ratio of peanut sprout makes the resveratrol in peanut sprout extract as much as possible, improves extraction efficiency.
Preferably, in step (2), the weight that lactic acid bacteria is added is the 5%~7% of mixed liquor A weight, and xylitol is added
Weight is the 5%~7% of mixed liquor A weight.In said extracted method, be added the lactic acid bacteria of preferred weight ratio and xylitol into
Row fermentation, can to the maximum extent extract resveratrol, recovery rate highest, extraction time is most short, and cost is minimum, together
When also act as adjust its mouthfeel effect.
Preferably, fermentation temperature is 30~45 DEG C in step (2), and fermentation time is 20~25h.Using above-mentioned preferred hair
Ensure that the subsequent extracted object of resveratrol will not be denaturalized while ferment temperature and time makes fermentation reaction fully carry out.
Preferably, in step (3), mixed liquid B is positioned in the micro-wave oven of 800~1000M Hz and is irradiated, irradiation time
For 10~15s.In said extracted method, mixed liquor is irradiated using the preferred frequency of micro-wave oven, promotes resveratrol recovery rate
Meanwhile avoid resveratrol in extract destroyed and extraction process in thermal loss.
Preferably, the extracting method of resveratrol is further comprising the steps of in peanut sprout：The extracting solution that step (3) is obtained
Carry out vacuum condensation concentration, you can obtain including the concentrate of resveratrol.When the white lamb's-quarters for needing high concentration in functional food
When reed alcohol, the resveratrol of required concentration can be obtained through the above steps.Above-mentioned vacuum condensation concentration step is being concentrated in vacuo
It is carried out in device, after resveratrol is concentrated in vacuo, cooling down operation is carried out directly in vacuum concentrator.Above-mentioned vacuum is cold
The method of solidifying concentration, improves thickening efficiency and cocnentration factor, concentrates cooling continuous operation, shortens the time of concentration, more often
The time of rule concentration can shorten 70% or more, while avoid destruction of the high temperature to ingredient in extract, and solvent steams
Hair amount maximum value can reach 90% or more.Said extracted method, reduces concentrated cost, substantially increases the dense of resveratrol
Contracting purity.
Preferably, temperature when vacuum condensation concentrates is 80~95 DEG C, the temperature of concentrate obtained after vacuum condensation concentration
Degree control is at 40 DEG C or less.Using temperature after above-mentioned preferred vacuum condensation thickening temperature and cooling, resveratrol can be made
Thickening efficiency reach maximum, and avoid the destruction of active ingredient in extract.
Preferably, the time of vacuum condensation concentration is 10~15h.The time of above-mentioned vacuum condensation concentration is compared to existing skill
The condensation time of 60h or more shortens many in art, and the time cost of extraction is greatly saved, improves the concentration of resveratrol
Efficiency.The pol of the concentrate comprising resveratrol is set to be 50~55brix after above-mentioned vacuum condensation concentration.
The preferred embodiment of the present invention additionally provide resveratrol in above-mentioned peanut sprout extracting method extract it is white
Veratryl alcohol.The resveratrol extracted using said extracted method is high-quality, not will produce the change of resveratrol active ingredient
Property or destruction, and have the faint scent of strong peanut fragrance and bud, uniform texture is sour-sweet moderate, delicate mouthfeel.After concentration
Resveratrol purity it is high, can be applied in the functional food material with high concentration resveratrol demand.
The preferred embodiment of the present invention additionally provide resveratrol in above-mentioned peanut sprout extracting method extract it is white
Application of the veratryl alcohol in drug and/or food.The resveratrol that said extracted method obtains can be applied to biological medicine neck
Domain, while food can also be applied to, have the characteristic of medicine-food two-purpose.Furthermore it is also possible to as food such as beverage, jelly
Assist added material.The resveratrol that said extracted method obtains appropriately is taken in daily life can improve human immunity
Function, effective prevention of cardiovascular disease, cancer, diabetes and Other diseases.
Embodiment
Chemical reagent is commercially available in following embodiment.
Embodiment 1
The extracting method of resveratrol, includes the following steps in the present embodiment peanut sprout：
(1) 15~20cm of length is chosen in the peanut sprout of Patent No. 201210455520.2, does not open leaf, is sturdy delicate
Organic ecosystem peanut sprout, go root to clean up above-mentioned peanut sprout, and smash and be squeezed into juice, be placed in 80 DEG C of hot water and soak
15h is steeped, mixed liquor A is obtained.Wherein, the weight of hot water is 3 times of peanut sprout weight.
(2) lactic acid bacteria, xylitol fermentation 25h are added in the mixed liquor A obtained to step (1), fermentation temperature maintains 38
DEG C, obtain mixed liquid B；Wherein the weight of lactic acid bacteria is the 5% of mixed liquor A weight, and the weight of xylitol is also mixed liquor A weight
The 5% of amount.
(3) mixed liquid B made from step (2), which is positioned in the micro-wave oven of 800M Hz, irradiates, irradiation time 10s, so
After extract resveratrol, obtain the extracting solution for including resveratrol.
(4) extracting solution that step (3) obtains is positioned in vacuum concentrator and carries out vacuum condensation concentration, vacuum condensation is dense
Temperature when contracting is 88 DEG C, after vacuum condensation concentration the temperature control of concentrate obtained at 40 DEG C, vacuum condensation concentration when
Between be 15h, you can obtain include the concentrate of resveratrol, wherein the pol of the concentrate of resveratrol is 55brix.
The recovery rate of the extracting method of resveratrol in the present embodiment peanut sprout, resveratrol is 98%.
Embodiment 2
The extracting method of resveratrol, includes the following steps in the present embodiment peanut sprout：
(1) 15~20cm of length is chosen in the peanut sprout of purchase, does not open leaf, sturdy delicate organic ecosystem peanut
Above-mentioned peanut sprout is gone root to clean up, and smashes and be squeezed into juice by bud, is placed in 80 DEG C of hot water and is impregnated 15h, obtains mixed liquor
A.Wherein, the weight of hot water is 3 times of peanut sprout weight.
(2) lactic acid bacteria, xylitol fermentation 25h are added in the mixed liquor A obtained to step (1), fermentation temperature maintains 38
DEG C, obtain mixed liquid B；Wherein the weight of lactic acid bacteria is the 5% of mixed liquor A weight, and the weight of xylitol is also mixed liquor A weight
The 5% of amount.
(3) mixed liquid B made from step (2), which is positioned in the micro-wave oven of 800MHz, irradiates, irradiation time 10s, then
Resveratrol is extracted, the extracting solution for including resveratrol is obtained.
(4) extracting solution that step (3) obtains is positioned in vacuum concentrator and carries out vacuum condensation concentration, vacuum condensation is dense
Temperature when contracting is 88 DEG C, after vacuum condensation concentration the temperature control of concentrate obtained at 40 DEG C, vacuum condensation concentration when
Between be 15h, you can obtain include the concentrate of resveratrol, wherein the pol of the concentrate of resveratrol is 50brix.
The recovery rate of the extracting method of resveratrol in the present embodiment peanut sprout, resveratrol is 95%.
Embodiment 3
The extracting method of resveratrol, includes the following steps in the present embodiment peanut sprout：
(1) 15~20cm of length is chosen in the peanut sprout of Patent No. 201210455520.2, does not open leaf, is sturdy delicate
Organic ecosystem peanut sprout, go root to clean up above-mentioned peanut sprout, and smash and be squeezed into juice, be placed in 95 DEG C of hot water and soak
10h is steeped, mixed liquor A is obtained.Wherein, the weight of hot water is 2 times of peanut sprout weight.
(2) lactic acid bacteria, xylitol fermentation 20h are added in the mixed liquor A obtained to step (1), fermentation temperature maintains 30
DEG C, obtain mixed liquid B；Wherein the weight of lactic acid bacteria is the 7% of mixed liquor A weight, and the weight of xylitol is also mixed liquor A weight
The 7% of amount.
(3) mixed liquid B made from step (2), which is positioned in the micro-wave oven of 1000M Hz, irradiates, irradiation time 15s, so
After extract resveratrol, obtain the extracting solution for including resveratrol.
(4) extracting solution that step (3) obtains is positioned in vacuum concentrator and carries out vacuum condensation concentration, vacuum condensation is dense
Temperature when contracting is 95 DEG C, after vacuum condensation concentration the temperature control of concentrate obtained at 40 DEG C, vacuum condensation concentration when
Between be 10h, you can obtain include the concentrate of resveratrol, wherein the pol of the concentrate of resveratrol is 55brix.
The recovery rate of the extracting method of resveratrol in the present embodiment peanut sprout, resveratrol is 97%.
Embodiment 4
The extracting method of resveratrol, includes the following steps in the present embodiment peanut sprout：
(1) 15~20cm of length is chosen in the peanut sprout of Patent No. 201210455520.2, does not open leaf, is sturdy delicate
Organic ecosystem peanut sprout, go root to clean up above-mentioned peanut sprout, and smash and be squeezed into juice, be placed in 90 DEG C of hot water and soak
12h is steeped, mixed liquor A is obtained.Wherein, the weight of hot water is 2.5 times of peanut sprout weight.
(2) lactic acid bacteria, xylitol fermentation 20h are added in the mixed liquor A obtained to step (1), fermentation temperature maintains 45
DEG C, obtain mixed liquid B；Wherein the weight of lactic acid bacteria is the 6% of mixed liquor A weight, and the weight of xylitol is also mixed liquor A weight
The 6% of amount.
(3) mixed liquid B made from step (2), which is positioned in the micro-wave oven of 900M Hz, irradiates, irradiation time 12s, so
After extract resveratrol, obtain the extracting solution for including resveratrol.
(4) extracting solution that step (3) obtains is positioned in vacuum concentrator and carries out vacuum condensation concentration, vacuum condensation is dense
Temperature when contracting is 80 DEG C, after vacuum condensation concentration the temperature control of concentrate obtained at 40 DEG C, vacuum condensation concentration when
Between be 12h, you can obtain include the concentrate of resveratrol, wherein the pol of the concentrate of resveratrol is 55brix.
The recovery rate of the extracting method of resveratrol in the present embodiment peanut sprout, resveratrol is 97%.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, for the skill of this field
For art personnel, the invention may be variously modified and varied.All within the spirits and principles of the present invention, any made by
Modification, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (10)
1. the extracting method of resveratrol in a kind of peanut sprout, which is characterized in that include the following steps：
(1) peanut sprout is smashed, is placed in hot water and impregnates, obtain mixed liquor A；
(2) lactic acid bacteria, xylitol fermentation are added in the mixed liquor A obtained to the step (1), obtains mixed liquid B；
(3) mixed liquid B made from the step (2) uses microwave irradiation, extracts resveratrol, obtains carrying comprising resveratrol
Take liquid.
2. the extracting method of resveratrol in peanut sprout according to claim 1, which is characterized in that
In the step (1), the temperature of the hot water is 80~95 DEG C, and soaking time is 10~15h.
3. the extracting method of resveratrol in peanut sprout according to claim 1, which is characterized in that
In the step (1), the weight of the hot water is 2~3 times of the peanut sprout weight.
4. the extracting method of resveratrol in peanut sprout according to claim 1, which is characterized in that
In the step (2), the weight that the lactic acid bacteria is added is the 5%~7% of the mixed liquor A weight, and the wood is added
The weight of sugar alcohol is the 5%~7% of the mixed liquor A weight.
5. the extracting method of resveratrol in peanut sprout according to claim 1, which is characterized in that
In the step (2), fermentation temperature is 30~45 DEG C, and fermentation time is 20~25h.
6. the extracting method of resveratrol in peanut sprout according to claim 1, which is characterized in that
In the step (3), the mixed liquid B is positioned in the micro-wave oven of 800~1000M Hz and is irradiated, irradiation time 10
~15s.
7. the extracting method of resveratrol in peanut sprout according to any one of claim 1 to 6, which is characterized in that
It is further comprising the steps of：The extracting solution that the step (3) is obtained carries out vacuum condensation concentration, you can obtains including white lamb's-quarters
The concentrate of reed alcohol.
8. the extracting method of resveratrol in peanut sprout according to claim 7, which is characterized in that
Temperature when the vacuum condensation concentration is 80~95 DEG C, the temperature control of the concentrate obtained after vacuum condensation concentration
System is at 40 DEG C or less；And/or
The time of the vacuum condensation concentration is 10~15h.
9. the resveratrol that the extracting method of resveratrol is extracted in peanut sprout according to any one of claims 1 to 8.
10. the resveratrol that the extracting method of resveratrol is extracted in peanut sprout according to any one of claims 1 to 8
Application in drug and/or food.
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| CN104003849A (en) * | 2013-09-17 | 2014-08-27 | 黄桂淑 | Extraction method of resveratrol from peanut buds |
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2018
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| JP2009040695A (en) * | 2007-08-07 | 2009-02-26 | Mitsuyuki Nakajima | Method for extracting and producing useful component derived from plant using aqueous solution containing catechin and/or vitamin c as solvent, extract, and fermentation product thereof |
| CN101353675A (en) * | 2008-08-15 | 2009-01-28 | 山东省花生研究所 | Method for extracting resveratrol by fermenting peanut root using aspergillus niger |
| CN101597214A (en) * | 2009-07-10 | 2009-12-09 | 山东省花生研究所 | A kind of method that extracts trans-resveratrol in the peanut root |
| CN104003849A (en) * | 2013-09-17 | 2014-08-27 | 黄桂淑 | Extraction method of resveratrol from peanut buds |
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Effective date of registration: 20210303 Address after: 410000 No.7, Guanjia group, zengtan village, chuangwan Town, Liling City, Zhuzhou City, Hunan Province Applicant after: Hunan Zhengfa Biotechnology Co.,Ltd. Address before: 2101, building B, Shanghe International Commercial Plaza, no.358, Section 1, Wanjiali Middle Road, Yuhua District, Changsha City, Hunan Province, 410000 Applicant before: CHANGSHA ZHENGFA AGRICULTURAL DEVELOPMENT Co.,Ltd. |